AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09588

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09588.t1 MTR_5g015720 95.943 419 15 1 1 419 1 417 0.0 815
MS.gene09588.t1 MTR_1g056640 27.880 434 268 12 23 416 62 490 2.09e-38 145
MS.gene09588.t1 MTR_1g116870 60.215 93 35 2 325 417 270 360 5.55e-31 122
MS.gene09588.t1 MTR_3g082080 60.215 93 35 2 326 418 152 242 1.25e-30 118
MS.gene09588.t1 MTR_7g053500 24.711 433 261 11 24 416 51 458 2.04e-28 116
MS.gene09588.t1 MTR_8g012400 25.701 428 252 15 28 416 58 458 8.74e-28 115
MS.gene09588.t1 MTR_8g012400 26.980 404 229 15 52 416 2 378 1.32e-27 113
MS.gene09588.t1 MTR_5g085580 54.651 86 38 1 331 416 382 466 1.15e-26 111
MS.gene09588.t1 MTR_7g053500 25.432 405 237 11 52 416 2 381 3.09e-26 109
MS.gene09588.t1 MTR_5g097420 54.545 88 39 1 329 416 28 114 1.42e-24 98.2
MS.gene09588.t1 MTR_8g085390 52.326 86 39 2 331 416 30 113 1.60e-23 97.4
MS.gene09588.t1 MTR_7g111600 53.488 86 38 2 331 416 160 243 4.28e-23 98.2
MS.gene09588.t1 MTR_8g085720 49.425 87 43 1 330 416 363 448 7.86e-23 100
MS.gene09588.t1 MTR_7g111600 53.488 86 38 2 331 416 160 243 1.07e-22 97.8
MS.gene09588.t1 MTR_7g111600 37.766 188 92 6 248 416 62 243 1.53e-22 97.8
MS.gene09588.t1 MTR_4g133735 50.000 86 42 1 331 416 28 112 2.21e-22 92.0
MS.gene09588.t1 MTR_8g089400 51.163 86 41 1 331 416 37 121 6.37e-22 92.0
MS.gene09588.t1 MTR_3g048280 52.500 80 36 2 331 410 112 189 6.81e-22 93.6
MS.gene09588.t1 MTR_8g089400 51.163 86 41 1 331 416 57 141 1.95e-21 91.3
MS.gene09588.t1 MTR_5g091650 50.000 86 41 2 331 416 108 191 5.85e-21 90.5
MS.gene09588.t1 MTR_1g084820 60.494 81 27 4 331 410 420 496 5.96e-21 95.5
MS.gene09588.t1 MTR_1g084820 60.494 81 27 4 331 410 420 496 6.88e-21 95.5
MS.gene09588.t1 MTR_8g085400 50.000 88 39 3 324 411 26 108 1.14e-20 87.4
MS.gene09588.t1 MTR_5g091650 50.000 86 41 2 331 416 108 191 1.38e-20 91.3
MS.gene09588.t1 MTR_4g132280 47.727 88 45 1 329 416 380 466 2.21e-20 93.6
MS.gene09588.t1 MTR_4g133730 45.455 88 43 2 331 416 28 112 4.17e-20 85.9
MS.gene09588.t1 MTR_8g085440 46.591 88 45 2 329 416 30 115 5.52e-20 85.5
MS.gene09588.t1 MTR_4g079530 46.067 89 47 1 328 416 34 121 6.81e-19 82.8
MS.gene09588.t1 MTR_4g088155 23.474 426 269 13 28 416 52 457 7.10e-19 89.0
MS.gene09588.t1 MTR_3g083580 45.455 88 47 1 329 416 360 446 7.61e-19 89.0
MS.gene09588.t1 MTR_4g079530 46.067 89 47 1 328 416 34 121 1.58e-18 83.2
MS.gene09588.t1 MTR_2g049670 51.685 89 40 3 331 419 158 243 1.65e-18 86.3
MS.gene09588.t1 MTR_4g109490 49.462 93 40 4 326 416 19 106 4.45e-18 82.8
MS.gene09588.t1 MTR_8g102340 42.222 90 51 1 328 417 331 419 5.09e-18 86.3
MS.gene09588.t1 MTR_1g031900 44.944 89 48 1 328 416 358 445 8.52e-18 85.9
MS.gene09588.t1 MTR_4g069940 40.000 110 62 2 310 416 351 459 9.49e-18 85.5
MS.gene09588.t1 MTR_1g031900 45.455 88 47 1 329 416 359 445 1.21e-17 85.1
MS.gene09588.t1 MTR_6g088885 43.182 88 48 2 331 417 369 455 1.67e-17 84.7
MS.gene09588.t1 MTR_8g085420 45.783 83 43 2 329 411 30 110 1.92e-17 79.0
MS.gene09588.t1 MTR_4g083500 44.318 88 47 2 329 416 400 485 4.75e-17 83.6
MS.gene09588.t1 MTR_5g078200 42.045 88 50 1 329 416 325 411 1.85e-16 82.0
MS.gene09588.t1 MTR_8g088120 52.703 74 33 2 343 416 60 131 1.87e-16 78.6
MS.gene09588.t1 MTR_5g078200 42.045 88 50 1 329 416 358 444 2.17e-16 81.6
MS.gene09588.t1 MTR_5g044530 50.685 73 34 2 330 402 21 91 4.39e-16 78.2
MS.gene09588.t1 MTR_4g083070 47.945 73 36 2 330 402 20 90 2.59e-15 74.3
MS.gene09588.t1 MTR_3g065460 42.529 87 47 2 331 416 361 445 9.88e-15 76.3
MS.gene09588.t1 MTR_1g022160 44.186 86 46 2 331 416 391 474 1.97e-14 75.5
MS.gene09588.t1 MTR_3g116510 41.860 86 48 2 331 416 237 320 2.78e-14 73.9
MS.gene09588.t1 MTR_3g116510 41.860 86 48 2 331 416 371 454 3.16e-14 74.7
MS.gene09588.t1 MTR_1g022160 43.023 86 47 2 331 416 391 474 4.22e-14 74.3
MS.gene09588.t1 MTR_2g072500 39.080 87 50 1 328 414 5 88 9.51e-13 64.3
MS.gene09588.t1 MTR_1g022160 44.304 79 42 2 331 409 391 467 1.52e-12 69.3
MS.gene09588.t1 MTR_7g026340 35.000 100 63 2 316 415 350 447 3.78e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09588.t1 AT5G67460 42.227 431 177 17 6 419 5 380 1.07e-82 259
MS.gene09588.t1 AT5G67460 41.647 425 176 17 6 413 5 374 1.52e-77 245
MS.gene09588.t1 AT5G55180 29.108 426 250 14 23 416 51 456 1.27e-39 147
MS.gene09588.t1 AT5G55180 29.286 420 245 14 23 410 51 450 9.86e-38 142
MS.gene09588.t1 AT5G56590 26.794 418 272 12 22 417 49 454 5.84e-32 127
MS.gene09588.t1 AT1G79480 58.065 93 37 2 324 416 303 393 8.17e-31 122
MS.gene09588.t1 AT1G79480 58.065 93 37 2 324 416 304 394 8.38e-31 122
MS.gene09588.t1 AT2G05790 58.140 86 35 1 331 416 384 468 2.56e-27 113
MS.gene09588.t1 AT4G26830 58.140 86 35 1 331 416 371 455 2.34e-26 110
MS.gene09588.t1 AT4G26830 58.140 86 35 1 331 416 367 451 2.49e-26 110
MS.gene09588.t1 AT2G30933 57.143 91 37 2 326 416 77 165 3.91e-26 105
MS.gene09588.t1 AT2G30933 58.824 85 33 2 326 410 77 159 7.80e-25 100
MS.gene09588.t1 AT2G30933 58.824 85 33 2 326 410 77 159 7.80e-25 100
MS.gene09588.t1 AT5G35740 48.837 86 44 0 331 416 30 115 1.89e-24 97.8
MS.gene09588.t1 AT3G07320 25.175 429 262 16 24 416 50 455 9.38e-24 103
MS.gene09588.t1 AT1G66855 45.455 88 47 1 329 416 24 110 1.27e-22 92.4
MS.gene09588.t1 AT2G04910 47.674 86 44 1 331 416 15 99 6.13e-22 90.5
MS.gene09588.t1 AT4G05430 46.512 86 45 1 331 416 23 107 3.11e-21 89.7
MS.gene09588.t1 AT2G04910 48.750 80 40 1 331 410 15 93 3.15e-21 89.0
MS.gene09588.t1 AT1G11820 48.864 88 44 1 329 416 379 465 3.20e-21 96.3
MS.gene09588.t1 AT4G05430 46.512 86 45 1 331 416 48 132 3.73e-21 90.5
MS.gene09588.t1 AT1G29380 54.023 87 36 3 331 416 148 231 6.80e-21 92.0
MS.gene09588.t1 AT4G13600 49.425 87 42 2 330 416 60 144 7.09e-21 91.3
MS.gene09588.t1 AT1G29380 54.023 87 36 3 331 416 148 231 8.32e-21 92.8
MS.gene09588.t1 AT1G66870 43.182 88 49 1 329 416 24 110 9.23e-21 87.4
MS.gene09588.t1 AT1G66870 43.182 88 49 1 329 416 51 137 1.52e-20 87.8
MS.gene09588.t1 AT1G11820 50.000 84 41 1 329 412 379 461 2.07e-20 93.6
MS.gene09588.t1 AT1G18650 51.163 86 40 2 331 416 21 104 3.25e-20 88.2
MS.gene09588.t1 AT5G61130 48.837 86 42 2 331 416 21 104 3.38e-20 88.6
MS.gene09588.t1 AT4G05430 48.750 80 40 1 331 410 48 126 3.91e-20 86.3
MS.gene09588.t1 AT3G13560 46.667 90 47 1 330 419 361 449 3.97e-20 92.8
MS.gene09588.t1 AT3G13560 46.667 90 47 1 330 419 361 449 3.97e-20 92.8
MS.gene09588.t1 AT3G13560 46.667 90 47 1 330 419 361 449 3.97e-20 92.8
MS.gene09588.t1 AT3G13560 46.667 90 47 1 330 419 361 449 3.97e-20 92.8
MS.gene09588.t1 AT3G13560 46.667 90 47 1 330 419 361 449 3.97e-20 92.8
MS.gene09588.t1 AT1G18650 51.163 86 40 2 331 416 21 104 4.22e-20 86.3
MS.gene09588.t1 AT1G29380 55.556 81 32 3 331 410 148 225 7.57e-20 88.2
MS.gene09588.t1 AT1G29380 55.556 81 32 3 331 410 148 225 7.57e-20 88.2
MS.gene09588.t1 AT2G01630 45.455 88 47 1 329 416 358 444 1.30e-19 91.3
MS.gene09588.t1 AT1G09460 51.163 86 38 3 331 416 137 218 3.59e-19 88.2
MS.gene09588.t1 AT2G01630 45.783 83 44 1 329 411 358 439 5.66e-19 89.0
MS.gene09588.t1 AT5G08000 48.889 90 44 2 327 416 36 123 2.09e-18 84.0
MS.gene09588.t1 AT5G08000 50.000 86 41 2 331 416 21 104 4.16e-18 82.4
MS.gene09588.t1 AT5G63225 46.429 84 43 2 331 414 25 106 9.03e-18 79.0
MS.gene09588.t1 AT4G29360 34.646 127 82 1 293 419 353 478 9.22e-18 85.9
MS.gene09588.t1 AT4G29360 30.464 151 95 3 24 168 52 198 4.65e-11 65.1
MS.gene09588.t1 AT4G29360 34.646 127 82 1 293 419 363 488 9.34e-18 85.9
MS.gene09588.t1 AT4G29360 30.464 151 95 3 24 168 62 208 4.47e-11 65.1
MS.gene09588.t1 AT5G63230 42.045 88 49 2 329 416 24 109 1.76e-17 78.2
MS.gene09588.t1 AT1G66852 39.773 88 51 2 329 416 24 109 2.31e-17 77.8
MS.gene09588.t1 AT3G55430 39.535 86 50 2 331 416 366 449 4.49e-17 83.2
MS.gene09588.t1 AT2G43670 41.667 84 46 2 331 414 35 115 3.58e-16 75.1
MS.gene09588.t1 AT4G16165 35.227 88 56 1 329 416 24 110 5.39e-16 73.9
MS.gene09588.t1 AT5G53610 45.349 86 44 2 331 416 28 110 6.58e-16 73.9
MS.gene09588.t1 AT5G63250 40.541 111 56 4 309 417 27 129 6.62e-16 74.3
MS.gene09588.t1 AT4G09090 40.230 87 50 2 331 417 31 115 8.75e-16 73.6
MS.gene09588.t1 AT2G29735 41.111 90 49 3 329 416 29 116 1.05e-15 73.6
MS.gene09588.t1 AT4G09462 38.636 88 52 2 329 416 27 112 1.56e-15 72.8
MS.gene09588.t1 AT4G09465 38.636 88 52 2 329 416 29 114 1.64e-15 72.8
MS.gene09588.t1 AT4G09467 38.636 88 52 2 329 416 29 114 1.88e-15 72.8
MS.gene09588.t1 AT4G09464 38.636 88 52 2 329 416 29 114 1.88e-15 72.8
MS.gene09588.t1 AT4G34480 43.023 86 47 2 331 416 253 336 2.97e-15 77.4
MS.gene09588.t1 AT4G34480 43.023 86 47 2 331 416 364 447 3.12e-15 77.8
MS.gene09588.t1 AT4G09466 37.500 88 53 2 329 416 29 114 5.81e-15 71.2
MS.gene09588.t1 AT1G66250 39.326 89 53 1 328 416 365 452 1.05e-14 76.3
MS.gene09588.t1 AT2G16230 37.864 103 61 3 315 416 349 449 1.92e-14 75.5
MS.gene09588.t1 AT3G23770 39.080 87 52 1 330 416 386 471 2.42e-14 75.1
MS.gene09588.t1 AT5G53600 36.559 93 56 2 324 416 22 111 3.03e-14 69.3
MS.gene09588.t1 AT1G69295 48.611 72 34 3 331 402 21 89 4.08e-14 71.6
MS.gene09588.t1 AT3G58100 39.773 88 52 1 329 416 39 125 5.45e-14 70.5
MS.gene09588.t1 AT1G26450 53.226 62 27 2 343 404 32 91 5.64e-14 70.9
MS.gene09588.t1 AT1G69295 48.611 72 34 3 331 402 17 85 6.03e-14 71.2
MS.gene09588.t1 AT3G58100 39.773 88 52 1 329 416 115 201 1.10e-13 71.2
MS.gene09588.t1 AT5G63240 32.039 103 67 2 317 417 26 127 2.22e-13 67.4
MS.gene09588.t1 AT2G43660 36.957 92 57 1 325 416 28 118 2.30e-13 67.0
MS.gene09588.t1 AT2G43660 36.957 92 57 1 325 416 29 119 2.33e-13 67.0
MS.gene09588.t1 AT5G24318 37.736 106 59 4 315 416 214 316 5.61e-13 69.7
MS.gene09588.t1 AT5G24318 37.736 106 59 4 315 416 353 455 6.05e-13 70.9
MS.gene09588.t1 AT1G13830 42.105 76 42 2 327 402 31 104 1.22e-12 67.4
MS.gene09588.t1 AT1G13830 43.836 73 39 2 330 402 20 90 2.12e-12 66.2
MS.gene09588.t1 AT1G78520 32.000 100 65 1 317 416 18 114 4.24e-12 63.2
MS.gene09588.t1 AT5G24318 38.835 103 56 4 315 413 353 452 6.25e-12 67.4
MS.gene09588.t1 AT3G28250 40.000 80 45 2 331 410 3 79 9.51e-12 62.4
MS.gene09588.t1 AT2G03505 38.750 80 47 2 331 410 21 98 2.17e-11 62.8
MS.gene09588.t1 AT2G03505 38.750 80 47 2 331 410 21 98 2.17e-11 62.8
MS.gene09588.t1 AT2G03505 38.750 80 47 2 331 410 21 98 2.17e-11 62.8
MS.gene09588.t1 AT2G03505 38.750 80 47 2 331 410 21 98 2.43e-11 61.2
MS.gene09588.t1 AT2G42930 37.113 97 58 3 314 410 23 116 4.96e-11 60.8

Find 78 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGTTTGCATTGCAGTTTC+TGG 0.188078 5.2:+7482012 MS.gene09588:intron
TTGTTCTTGAGGGGCAAAAT+AGG 0.257528 5.2:-7482802 None:intergenic
CTTATGCATTCAATAGTTAC+TGG 0.260747 5.2:+7483108 MS.gene09588:CDS
GTTGAGCAATTTCTGGAATT+GGG 0.288063 5.2:-7482665 None:intergenic
GGTTGAGCAATTTCTGGAAT+TGG 0.309049 5.2:-7482666 None:intergenic
GCAAAATAGGGAGAAGAAAT+TGG 0.319946 5.2:-7482789 None:intergenic
GAACAACATTAAGGTTGTTT+TGG 0.320840 5.2:-7482266 None:intergenic
CCTTGCAATGCAGTAGAAAA+TGG 0.325726 5.2:+7482891 MS.gene09588:CDS
CATCTTCTCTTTCTTTGCTC+TGG 0.345679 5.2:+7481825 MS.gene09588:CDS
TTTGAATTTGGTACATTGTA+AGG 0.345996 5.2:-7482726 None:intergenic
CCTGCTAACTCTCCTCAAAA+TGG 0.348344 5.2:+7482855 MS.gene09588:CDS
CACTTGTTTCACATAGGTTT+AGG 0.352249 5.2:-7482053 None:intergenic
ATGGTGATGGTTGTTCTTGA+GGG 0.357121 5.2:-7482812 None:intergenic
TGAAGGTGGTGCTGATTGTT+TGG 0.361551 5.2:+7483028 MS.gene09588:CDS
GCATGAGCAACCAATGTATC+AGG 0.366680 5.2:-7483083 None:intergenic
ACAAGAAAAGAAAATGAAAA+TGG 0.371125 5.2:+7481791 None:intergenic
GGTTTGAAGTAGCTGAGTTA+AGG 0.381419 5.2:-7482389 None:intergenic
TGAAACGTTGCAAGAAGCTT+TGG 0.385008 5.2:+7482992 MS.gene09588:CDS
GCTTGAAGAAACTCAATCAT+AGG 0.387058 5.2:-7482432 None:intergenic
TGTTCTTGAGGGGCAAAATA+GGG 0.397503 5.2:-7482801 None:intergenic
ACAGAAGTAGAACAACATTA+AGG 0.399975 5.2:-7482275 None:intergenic
TTTCTCTCATACCAAGTTCA+AGG 0.409782 5.2:+7482469 MS.gene09588:CDS
CAATGTTTATGATATTGTTA+TGG 0.410294 5.2:-7482569 None:intergenic
GTTTGAAGTAGCTGAGTTAA+GGG 0.410988 5.2:-7482388 None:intergenic
TGGCAAAAGCATAAGAGAAT+TGG 0.415770 5.2:+7483128 MS.gene09588:CDS
GCTTCTTGCAACGTTTCATC+AGG 0.416313 5.2:-7482987 None:intergenic
TTCTGAACAGGTGCAATTTG+TGG 0.425677 5.2:-7482936 None:intergenic
TTGCACCTGTTCAGAAACTT+TGG 0.430193 5.2:+7482943 MS.gene09588:CDS
TCTATCATTCCCTCAAAAGA+TGG 0.439369 5.2:+7482313 MS.gene09588:CDS
GATGAAACTATTTGAGCTAA+AGG 0.452012 5.2:-7482759 None:intergenic
TATCAGCTGAAACTTGGCTT+AGG 0.457511 5.2:+7482166 MS.gene09588:CDS
TTGCAGGTTTCCTTCACTGT+AGG 0.460667 5.2:+7483507 MS.gene09588:intron
GCAATTTCTGGAATTGGGGT+TGG 0.460853 5.2:-7482660 None:intergenic
TTTGGTACATTGTAAGGAGC+TGG 0.460933 5.2:-7482720 None:intergenic
ATTTGAGCTAAAGGTGGTAA+AGG 0.464246 5.2:-7482750 None:intergenic
TTGGATTATGCTTGTGGTGA+AGG 0.471347 5.2:+7483011 MS.gene09588:CDS
AATGGTGATGGTTGTTCTTG+AGG 0.476899 5.2:-7482813 None:intergenic
ATTGGTGGAACTTGTGACTT+TGG 0.478733 5.2:+7483146 MS.gene09588:CDS
ACACACCAAAGTTTCTGAAC+AGG 0.478993 5.2:-7482948 None:intergenic
TTGTTACAATCCTGATACAT+TGG 0.480675 5.2:+7483073 MS.gene09588:CDS
CTATCATTCCCTCAAAAGAT+GGG 0.481726 5.2:+7482314 MS.gene09588:CDS
GTGGAACTTGTGACTTTGGT+GGG 0.482171 5.2:+7483150 MS.gene09588:CDS
TGTTGCTAGAGACCTTGTTA+AGG 0.501164 5.2:-7482140 None:intergenic
ACATTGTAAGGAGCTGGATA+AGG 0.502108 5.2:-7482714 None:intergenic
CAAAAGCATAAGAGAATTGG+TGG 0.510123 5.2:+7483131 MS.gene09588:CDS
AAATGTGAAAACCTTGAACT+TGG 0.511512 5.2:-7482480 None:intergenic
TTGAGCAATTTCTGGAATTG+GGG 0.520319 5.2:-7482664 None:intergenic
TTGTTCAAAGAAACAGCTAA+AGG 0.528055 5.2:-7482111 None:intergenic
GATACAAACTGTGGTGGTAA+TGG 0.537334 5.2:-7482831 None:intergenic
AACCAGCAGGGAAATCCAAA+GGG 0.537740 5.2:-7482689 None:intergenic
TAGGGAGAAGAAATTGGTGG+TGG 0.538044 5.2:-7482783 None:intergenic
GAACCAGCAGGGAAATCCAA+AGG 0.539386 5.2:-7482690 None:intergenic
GCTGGATAAGGAGAACCAGC+AGG 0.554077 5.2:-7482702 None:intergenic
TGTCTTCACTTGTTTCACAT+AGG 0.568580 5.2:-7482059 None:intergenic
ACAGTCCAAATCAAAAGCAA+TGG 0.570988 5.2:-7482361 None:intergenic
GTCTTGTTGGCAGAAAGTTG+AGG 0.577252 5.2:-7482244 None:intergenic
GATTATGCTTGTGGTGAAGG+TGG 0.582581 5.2:+7483014 MS.gene09588:CDS
GGTGGAACTTGTGACTTTGG+TGG 0.584114 5.2:+7483149 MS.gene09588:CDS
AACGTTTCATCAGGAACACT+AGG 0.602336 5.2:-7482978 None:intergenic
GAAGCTTTGGATTATGCTTG+TGG 0.604890 5.2:+7483005 MS.gene09588:CDS
GGATTGTAACAATTCCCTTG+TGG 0.608596 5.2:-7483062 None:intergenic
TTGGAGATTACTACTCCACA+AGG 0.615549 5.2:+7483047 MS.gene09588:CDS
TGAGAATAAACCTACAGTGA+AGG 0.617438 5.2:-7483517 None:intergenic
AAAAGAAAATGAAAATGGTG+TGG 0.631353 5.2:+7481796 None:intergenic
TATCATTCCCTCAAAAGATG+GGG 0.632855 5.2:+7482315 MS.gene09588:CDS
AACTGTGGTGGTAATGGTGA+TGG 0.632946 5.2:-7482825 None:intergenic
GAAACTATTTGAGCTAAAGG+TGG 0.639226 5.2:-7482756 None:intergenic
TGGTGATGGTTGTTCTTGAG+GGG 0.643729 5.2:-7482811 None:intergenic
AAATAGGGAGAAGAAATTGG+TGG 0.650191 5.2:-7482786 None:intergenic
GCAATTTGTGGATAATGTGA+AGG 0.661579 5.2:-7482924 None:intergenic
TGTGGATAATGTGAAGGTGA+AGG 0.662098 5.2:-7482918 None:intergenic
GAACAATGAAGACCTTAACA+AGG 0.665682 5.2:+7482128 MS.gene09588:CDS
CTGGATAAGGAGAACCAGCA+GGG 0.668605 5.2:-7482701 None:intergenic
GCAGGAGATACAAACTGTGG+TGG 0.675527 5.2:-7482837 None:intergenic
ACCAGCAGGGAAATCCAAAG+GGG 0.725497 5.2:-7482688 None:intergenic
TGGAGATTACTACTCCACAA+GGG 0.734669 5.2:+7483048 MS.gene09588:CDS
CCAGCAGGGAAATCCAAAGG+GGG 0.739134 5.2:-7482687 None:intergenic
TTAGCAGGAGATACAAACTG+TGG 0.759496 5.2:-7482840 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAAGTGCTTATTAATTT+AGG + chr5.2:7483199-7483218 MS.gene09588:intron 20.0%
!! CAATGTTTATGATATTGTTA+TGG - chr5.2:7482572-7482591 None:intergenic 20.0%
!! TAAATTAATAAGCACTTACT+TGG - chr5.2:7483200-7483219 None:intergenic 20.0%
!! TGTTAATTAATAATGAGTGA+TGG + chr5.2:7483250-7483269 MS.gene09588:intron 20.0%
!! TTACATTAATAATGGTGAAT+TGG + chr5.2:7483473-7483492 MS.gene09588:intron 20.0%
!!! AATTAATTAGCTGTTTTAGA+TGG - chr5.2:7483422-7483441 None:intergenic 20.0%
!!! GTTTTAACATTTTCAAATCA+TGG - chr5.2:7482413-7482432 None:intergenic 20.0%
! ATAAAGTATGTAACTACCAA+AGG + chr5.2:7483381-7483400 MS.gene09588:intron 25.0%
! TATAGTTATTGTACATAGCA+GGG - chr5.2:7483364-7483383 None:intergenic 25.0%
! TTAGAATCCATGAAAAAACT+TGG + chr5.2:7482537-7482556 MS.gene09588:CDS 25.0%
! TTATAGTTATTGTACATAGC+AGG - chr5.2:7483365-7483384 None:intergenic 25.0%
! TTTGAATTTGGTACATTGTA+AGG - chr5.2:7482729-7482748 None:intergenic 25.0%
!! ATTTTTGATGAAGGGTAATA+TGG - chr5.2:7482204-7482223 None:intergenic 25.0%
!! TAGTGATTTTTCTCTCTTTT+GGG - chr5.2:7482605-7482624 None:intergenic 25.0%
!! TTTTTGATGAAGGGTAATAT+GGG - chr5.2:7482203-7482222 None:intergenic 25.0%
!!! GAAAAATCCAAGTTTTTTCA+TGG - chr5.2:7482547-7482566 None:intergenic 25.0%
AAATGTGAAAACCTTGAACT+TGG - chr5.2:7482483-7482502 None:intergenic 30.0%
ACAGAAGTAGAACAACATTA+AGG - chr5.2:7482278-7482297 None:intergenic 30.0%
CTTATGCATTCAATAGTTAC+TGG + chr5.2:7483108-7483127 MS.gene09588:CDS 30.0%
GATGAAACTATTTGAGCTAA+AGG - chr5.2:7482762-7482781 None:intergenic 30.0%
TCAAAAATCACCAACATTGT+TGG + chr5.2:7482216-7482235 MS.gene09588:CDS 30.0%
TTGTTACAATCCTGATACAT+TGG + chr5.2:7483073-7483092 MS.gene09588:CDS 30.0%
TTGTTCAAAGAAACAGCTAA+AGG - chr5.2:7482114-7482133 None:intergenic 30.0%
! ACATTTTATCAGCTGAAACT+TGG + chr5.2:7482160-7482179 MS.gene09588:CDS 30.0%
! GAACAACATTAAGGTTGTTT+TGG - chr5.2:7482269-7482288 None:intergenic 30.0%
! GTAGTGATTTTTCTCTCTTT+TGG - chr5.2:7482606-7482625 None:intergenic 30.0%
! TTAGATGGTTACAGTTTGTT+AGG - chr5.2:7483407-7483426 None:intergenic 30.0%
!! AGTTTCCATTGCTTTTGATT+TGG + chr5.2:7482356-7482375 MS.gene09588:CDS 30.0%
!! ATTGGTTATTGTTTTGTTGC+AGG + chr5.2:7483491-7483510 MS.gene09588:intron 30.0%
!!! ATGTTGGTGATTTTTGATGA+AGG - chr5.2:7482213-7482232 None:intergenic 30.0%
!!! TGTTGGTGATTTTTGATGAA+GGG - chr5.2:7482212-7482231 None:intergenic 30.0%
!!! TTTTCTCTCTTTTGGGATAA+TGG - chr5.2:7482598-7482617 None:intergenic 30.0%
AAATAGGGAGAAGAAATTGG+TGG - chr5.2:7482789-7482808 None:intergenic 35.0%
ACAGTCCAAATCAAAAGCAA+TGG - chr5.2:7482364-7482383 None:intergenic 35.0%
ATTTGAGCTAAAGGTGGTAA+AGG - chr5.2:7482753-7482772 None:intergenic 35.0%
CACTTGTTTCACATAGGTTT+AGG - chr5.2:7482056-7482075 None:intergenic 35.0%
CTATCATTCCCTCAAAAGAT+GGG + chr5.2:7482314-7482333 MS.gene09588:CDS 35.0%
GAAACTATTTGAGCTAAAGG+TGG - chr5.2:7482759-7482778 None:intergenic 35.0%
GAACAATGAAGACCTTAACA+AGG + chr5.2:7482128-7482147 MS.gene09588:CDS 35.0%
GCAAAATAGGGAGAAGAAAT+TGG - chr5.2:7482792-7482811 None:intergenic 35.0%
GCAATTTGTGGATAATGTGA+AGG - chr5.2:7482927-7482946 None:intergenic 35.0%
GCTTGAAGAAACTCAATCAT+AGG - chr5.2:7482435-7482454 None:intergenic 35.0%
TATCATTCCCTCAAAAGATG+GGG + chr5.2:7482315-7482334 MS.gene09588:CDS 35.0%
TCTATCATTCCCTCAAAAGA+TGG + chr5.2:7482313-7482332 MS.gene09588:CDS 35.0%
TGAGAATAAACCTACAGTGA+AGG - chr5.2:7483520-7483539 None:intergenic 35.0%
TGTCTTCACTTGTTTCACAT+AGG - chr5.2:7482062-7482081 None:intergenic 35.0%
TTTCTCTCATACCAAGTTCA+AGG + chr5.2:7482469-7482488 MS.gene09588:CDS 35.0%
! CAAAAGCATAAGAGAATTGG+TGG + chr5.2:7483131-7483150 MS.gene09588:CDS 35.0%
! GTTTGAAGTAGCTGAGTTAA+GGG - chr5.2:7482391-7482410 None:intergenic 35.0%
! TGAGGTTTTTCCAACAATGT+TGG - chr5.2:7482229-7482248 None:intergenic 35.0%
! TGGCAAAAGCATAAGAGAAT+TGG + chr5.2:7483128-7483147 MS.gene09588:CDS 35.0%
! TTCTAAACCCCATCTTTTGA+GGG - chr5.2:7482325-7482344 None:intergenic 35.0%
!! GTTGAGCAATTTCTGGAATT+GGG - chr5.2:7482668-7482687 None:intergenic 35.0%
!! TTGAGCAATTTCTGGAATTG+GGG - chr5.2:7482667-7482686 None:intergenic 35.0%
!!! GGTGGTAAAGGTTTTGAATT+TGG - chr5.2:7482741-7482760 None:intergenic 35.0%
!!! GGTTGGTTTTATAGGAATTG+TGG - chr5.2:7482646-7482665 None:intergenic 35.0%
!!! TAAGGTTGTTTTGGTCTTGT+TGG - chr5.2:7482260-7482279 None:intergenic 35.0%
AACGTTTCATCAGGAACACT+AGG - chr5.2:7482981-7483000 None:intergenic 40.0%
ACACACCAAAGTTTCTGAAC+AGG - chr5.2:7482951-7482970 None:intergenic 40.0%
ACATTGTAAGGAGCTGGATA+AGG - chr5.2:7482717-7482736 None:intergenic 40.0%
AGTGATGGTTGTGTTGTGAA+TGG + chr5.2:7483265-7483284 MS.gene09588:intron 40.0%
ATTGGTGGAACTTGTGACTT+TGG + chr5.2:7483146-7483165 MS.gene09588:CDS 40.0%
CATCTTCTCTTTCTTTGCTC+TGG + chr5.2:7481825-7481844 MS.gene09588:CDS 40.0%
CCTTGCAATGCAGTAGAAAA+TGG + chr5.2:7482891-7482910 MS.gene09588:CDS 40.0%
GATACAAACTGTGGTGGTAA+TGG - chr5.2:7482834-7482853 None:intergenic 40.0%
GGATTGTAACAATTCCCTTG+TGG - chr5.2:7483065-7483084 None:intergenic 40.0%
TATCAGCTGAAACTTGGCTT+AGG + chr5.2:7482166-7482185 MS.gene09588:CDS 40.0%
TGAAACGTTGCAAGAAGCTT+TGG + chr5.2:7482992-7483011 MS.gene09588:CDS 40.0%
TGGAGATTACTACTCCACAA+GGG + chr5.2:7483048-7483067 MS.gene09588:CDS 40.0%
TGTGGATAATGTGAAGGTGA+AGG - chr5.2:7482921-7482940 None:intergenic 40.0%
TGTTCTTGAGGGGCAAAATA+GGG - chr5.2:7482804-7482823 None:intergenic 40.0%
TGTTGCTAGAGACCTTGTTA+AGG - chr5.2:7482143-7482162 None:intergenic 40.0%
TTAGCAGGAGATACAAACTG+TGG - chr5.2:7482843-7482862 None:intergenic 40.0%
TTCTGAACAGGTGCAATTTG+TGG - chr5.2:7482939-7482958 None:intergenic 40.0%
TTGCACCTGTTCAGAAACTT+TGG + chr5.2:7482943-7482962 MS.gene09588:CDS 40.0%
TTGGAGATTACTACTCCACA+AGG + chr5.2:7483047-7483066 MS.gene09588:CDS 40.0%
TTGGATTATGCTTGTGGTGA+AGG + chr5.2:7483011-7483030 MS.gene09588:CDS 40.0%
TTGTTCTTGAGGGGCAAAAT+AGG - chr5.2:7482805-7482824 None:intergenic 40.0%
TTTGGTACATTGTAAGGAGC+TGG - chr5.2:7482723-7482742 None:intergenic 40.0%
! CCATTTTCTACTGCATTGCA+AGG - chr5.2:7482894-7482913 None:intergenic 40.0%
! GGACAAGTGAAACCATTTTG+AGG - chr5.2:7482870-7482889 None:intergenic 40.0%
! GGTTTGAAGTAGCTGAGTTA+AGG - chr5.2:7482392-7482411 None:intergenic 40.0%
! GTAGTTTGCATTGCAGTTTC+TGG + chr5.2:7482012-7482031 MS.gene09588:intron 40.0%
! TTTTCTACTGCATTGCAAGG+TGG - chr5.2:7482891-7482910 None:intergenic 40.0%
!! AATGGTGATGGTTGTTCTTG+AGG - chr5.2:7482816-7482835 None:intergenic 40.0%
!! ATGGTGATGGTTGTTCTTGA+GGG - chr5.2:7482815-7482834 None:intergenic 40.0%
!! GAAGCTTTGGATTATGCTTG+TGG + chr5.2:7483005-7483024 MS.gene09588:CDS 40.0%
!! GGTTGAGCAATTTCTGGAAT+TGG - chr5.2:7482669-7482688 None:intergenic 40.0%
!! GTTACAGTTTGTTAGGCCTT+TGG - chr5.2:7483400-7483419 None:intergenic 40.0%
!! GTTCTAAACCCCATCTTTTG+AGG - chr5.2:7482326-7482345 None:intergenic 40.0%
!!! GGAATTGGGGTTGGTTTTAT+AGG - chr5.2:7482654-7482673 None:intergenic 40.0%
AAAGGGGGTTGAGCAATTTC+TGG - chr5.2:7482675-7482694 None:intergenic 45.0%
AACCAGCAGGGAAATCCAAA+GGG - chr5.2:7482692-7482711 None:intergenic 45.0%
AGAAATTGCTCAACCCCCTT+TGG + chr5.2:7482674-7482693 MS.gene09588:CDS 45.0%
CCTGCTAACTCTCCTCAAAA+TGG + chr5.2:7482855-7482874 MS.gene09588:CDS 45.0%
GATTATGCTTGTGGTGAAGG+TGG + chr5.2:7483014-7483033 MS.gene09588:CDS 45.0%
GCATGAGCAACCAATGTATC+AGG - chr5.2:7483086-7483105 None:intergenic 45.0%
GCTTCTTGCAACGTTTCATC+AGG - chr5.2:7482990-7483009 None:intergenic 45.0%
GTGGAACTTGTGACTTTGGT+GGG + chr5.2:7483150-7483169 MS.gene09588:CDS 45.0%
TAGGGAGAAGAAATTGGTGG+TGG - chr5.2:7482786-7482805 None:intergenic 45.0%
TTGCAGGTTTCCTTCACTGT+AGG + chr5.2:7483507-7483526 MS.gene09588:intron 45.0%
! GTCTTGTTGGCAGAAAGTTG+AGG - chr5.2:7482247-7482266 None:intergenic 45.0%
!! AACTGTGGTGGTAATGGTGA+TGG - chr5.2:7482828-7482847 None:intergenic 45.0%
!! CCATTTTGAGGAGAGTTAGC+AGG - chr5.2:7482858-7482877 None:intergenic 45.0%
!! GCAATTTCTGGAATTGGGGT+TGG - chr5.2:7482663-7482682 None:intergenic 45.0%
!! TGAAGGTGGTGCTGATTGTT+TGG + chr5.2:7483028-7483047 MS.gene09588:CDS 45.0%
!! TGGTGATGGTTGTTCTTGAG+GGG - chr5.2:7482814-7482833 None:intergenic 45.0%
!! TTATTTATTTACATTAATAA+TGG + chr5.2:7483465-7483484 MS.gene09588:intron 5.0%
ACCAGCAGGGAAATCCAAAG+GGG - chr5.2:7482691-7482710 None:intergenic 50.0%
CTGGATAAGGAGAACCAGCA+GGG - chr5.2:7482704-7482723 None:intergenic 50.0%
GAACCAGCAGGGAAATCCAA+AGG - chr5.2:7482693-7482712 None:intergenic 50.0%
GCAGGAGATACAAACTGTGG+TGG - chr5.2:7482840-7482859 None:intergenic 50.0%
GGTGGAACTTGTGACTTTGG+TGG + chr5.2:7483149-7483168 MS.gene09588:CDS 50.0%
CCAGCAGGGAAATCCAAAGG+GGG - chr5.2:7482690-7482709 None:intergenic 55.0%
GCTGGATAAGGAGAACCAGC+AGG - chr5.2:7482705-7482724 None:intergenic 55.0%
CCCCCTTTGGATTTCCCTGC+TGG + chr5.2:7482687-7482706 MS.gene09588:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 7481804 7483544 7481804 ID=MS.gene09588
chr5.2 mRNA 7481804 7483544 7481804 ID=MS.gene09588.t1;Parent=MS.gene09588
chr5.2 exon 7481804 7481846 7481804 ID=MS.gene09588.t1.exon1;Parent=MS.gene09588.t1
chr5.2 CDS 7481804 7481846 7481804 ID=cds.MS.gene09588.t1;Parent=MS.gene09588.t1
chr5.2 exon 7482016 7483200 7482016 ID=MS.gene09588.t1.exon2;Parent=MS.gene09588.t1
chr5.2 CDS 7482016 7483200 7482016 ID=cds.MS.gene09588.t1;Parent=MS.gene09588.t1
chr5.2 exon 7483513 7483544 7483513 ID=MS.gene09588.t1.exon3;Parent=MS.gene09588.t1
chr5.2 CDS 7483513 7483544 7483513 ID=cds.MS.gene09588.t1;Parent=MS.gene09588.t1
Gene Sequence

>MS.gene09588

ATGAAAATGGTGTGGCTATTTCATCTTCTCTTTCTTTGCTCTGGTAAGAAAAACAACTAAAATCTCAGTTTTGTAATTTCTCTTAAACTAAAATGTCTCAAAATATTCATCATCAATGTATCAATGTTTTAGTTTATTATTCTTCTTTTAAGTATTTTTTAACATTTTATTATTATTATTATCTAATGAAAGTTTTTGTTTTTTTGTTGTAGTTTGCATTGCAGTTTCTGGTCAACAAAACATTGAGTTCCTAAACCTATGTGAAACAAGTGAAGACATTGCAACATCTTCATCTCACAGTGAGTTTCCTTTAGCTGTTTCTTTGAACAATGAAGACCTTAACAAGGTCTCTAGCAACATTTTATCAGCTGAAACTTGGCTTAGGTTAAACATTTTCCCATATTACCCTTCATCAAAAATCACCAACATTGTTGGAAAAACCTCAACTTTCTGCCAACAAGACCAAAACAACCTTAATGTTGTTCTACTTCTGTCTTCTTTGAAAAATCTCTATCATTCCCTCAAAAGATGGGGTTTAGAACAAGACATCAAAGTTTCCATTGCTTTTGATTTGGACTGTTTTACCCTTAACTCAGCTACTTCAAACCATGATTTGAAAATGTTAAAACCTATGATTGAGTTTCTTCAAGCTGTTAACTCAACATTTTCTCTCATACCAAGTTCAAGGTTTTCACATTTTTCTGATAAAAGTTTAAACTTTGTGTCTTCTCAATTAGAATCCATGAAAAAACTTGGATTTTTCAACCATAACAATATCATAAACATTGCTACCATTATCCCAAAAGAGAGAAAAATCACTACTAGAAAACTTTCAGTTACCACAATTCCTATAAAACCAACCCCAATTCCAGAAATTGCTCAACCCCCTTTGGATTTCCCTGCTGGTTCTCCTTATCCAGCTCCTTACAATGTACCAAATTCAAAACCTTTACCACCTTTAGCTCAAATAGTTTCATCTCCACCACCAATTTCTTCTCCCTATTTTGCCCCTCAAGAACAACCATCACCATTACCACCACAGTTTGTATCTCCTGCTAACTCTCCTCAAAATGGTTTCACTTGTCCACCTTGCAATGCAGTAGAAAATGGTTCACCTTCACCTTCACATTATCCACAAATTGCACCTGTTCAGAAACTTTGGTGTGTTGCAAAGCCTAGTGTTCCTGATGAAACGTTGCAAGAAGCTTTGGATTATGCTTGTGGTGAAGGTGGTGCTGATTGTTTGGAGATTACTACTCCACAAGGGAATTGTTACAATCCTGATACATTGGTTGCTCATGCTTCTTATGCATTCAATAGTTACTGGCAAAAGCATAAGAGAATTGGTGGAACTTGTGACTTTGGTGGGACTGCTATGTTGATTCATTCTGATCCAAGTAAGTGCTTATTAATTTAGGATTAATTACAATTTTTTTACTTATACTTTGTTAATTAATAATGAGTGATGGTTGTGTTGTGAATGGTATGAATTTGTGATAGAGAAAAAAGAACTTTTCATTGTTACTGAGAATGAAAAATATTGATACATATACTATACCCTGCTATGTACAATAACTATAAAGTATGTAACTACCAAAGGCCTAACAAACTGTAACCATCTAAAACAGCTAATTAATTAACAAAATGTTAATCTAATCTATTTATTTATTTACATTAATAATGGTGAATTGGTTATTGTTTTGTTGCAGGTTTCCTTCACTGTAGGTTTATTCTCAGCTAG
Protein sequence

>MS.gene09588.t1

MKMVWLFHLLFLCSVCIAVSGQQNIEFLNLCETSEDIATSSSHSEFPLAVSLNNEDLNKVSSNILSAETWLRLNIFPYYPSSKITNIVGKTSTFCQQDQNNLNVVLLLSSLKNLYHSLKRWGLEQDIKVSIAFDLDCFTLNSATSNHDLKMLKPMIEFLQAVNSTFSLIPSSRFSHFSDKSLNFVSSQLESMKKLGFFNHNNIINIATIIPKERKITTRKLSVTTIPIKPTPIPEIAQPPLDFPAGSPYPAPYNVPNSKPLPPLAQIVSSPPPISSPYFAPQEQPSPLPPQFVSPANSPQNGFTCPPCNAVENGSPSPSHYPQIAPVQKLWCVAKPSVPDETLQEALDYACGEGGADCLEITTPQGNCYNPDTLVAHASYAFNSYWQKHKRIGGTCDFGGTAMLIHSDPSFLHCRFILS