AlfalfaGEDB | MS.gene056335

M. sativa cultivar XinJiangDaYe / MS.gene056335

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056335.t1	XP_004512510.2	100	136	0	0	1	136	104	239	5.80E-64	253.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056335.t1	P68429	100.0	136	0	0	1	136	1	136	1.4e-66	253.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056335.t1	A0A1S3TJX1	100.0	136	0	0	1	136	1	136	4.2e-64	253.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049622	MS.gene056335	0.825667	4.14E-54	-1.69E-46
MS.gene049989	MS.gene056335	0.824224	9.06E-54	-1.69E-46
MS.gene051263	MS.gene056335	0.819743	9.89E-53	-1.69E-46
MS.gene055957	MS.gene056335	0.878316	3.10E-69	-1.69E-46
MS.gene056042	MS.gene056335	0.819406	1.18E-52	-1.69E-46
MS.gene056335	MS.gene058197	0.800553	1.37E-48	-1.69E-46
MS.gene056335	MS.gene06057	0.809644	1.71E-50	-1.69E-46
MS.gene056335	MS.gene061805	0.808941	2.42E-50	-1.69E-46
MS.gene056335	MS.gene064080	0.801988	6.94E-49	-1.69E-46
MS.gene056335	MS.gene064081	0.80911	2.23E-50	-1.69E-46
MS.gene056335	MS.gene066535	0.811637	6.34E-51	-1.69E-46
MS.gene056335	MS.gene067143	0.817318	3.51E-52	-1.69E-46
MS.gene056335	MS.gene067144	0.812056	5.14E-51	-1.69E-46
MS.gene056335	MS.gene072702	0.819293	1.25E-52	-1.69E-46
MS.gene056335	MS.gene072956	0.810478	1.13E-50	-1.69E-46
MS.gene056335	MS.gene072963	0.806155	9.44E-50	-1.69E-46
MS.gene056335	MS.gene07616	0.826039	3.37E-54	-1.69E-46
MS.gene056335	MS.gene08836	0.824706	6.98E-54	-1.69E-46
MS.gene056335	MS.gene09014	0.804489	2.11E-49	-1.69E-46
MS.gene056335	MS.gene09234	0.801909	7.21E-49	-1.69E-46
MS.gene056335	MS.gene09258	0.822913	1.84E-53	-1.69E-46
MS.gene056335	MS.gene09540	0.802098	6.59E-49	-1.69E-46
MS.gene056335	MS.gene09543	0.800159	1.64E-48	-1.69E-46
MS.gene056335	MS.gene09588	0.803217	3.87E-49	-1.69E-46
MS.gene056335	MS.gene21029	0.813223	2.85E-51	-1.69E-46
MS.gene056335	MS.gene22500	0.802549	5.32E-49	-1.69E-46
MS.gene056335	MS.gene23323	0.820404	6.98E-53	-1.69E-46
MS.gene056335	MS.gene23980	0.806556	7.77E-50	-1.69E-46
MS.gene056335	MS.gene24543	0.800005	1.77E-48	-1.69E-46
MS.gene056335	MS.gene24735	0.803815	2.91E-49	-1.69E-46
MS.gene056335	MS.gene28050	0.807989	3.86E-50	-1.69E-46
MS.gene056335	MS.gene28438	0.818429	1.97E-52	-1.69E-46
MS.gene056335	MS.gene28807	0.800523	1.38E-48	-1.69E-46
MS.gene056335	MS.gene28867	0.811749	5.99E-51	-1.69E-46
MS.gene056335	MS.gene32703	0.809393	1.93E-50	-1.69E-46
MS.gene056335	MS.gene33688	0.834123	3.60E-56	-1.69E-46
MS.gene056335	MS.gene35715	0.800826	1.20E-48	-1.69E-46
MS.gene056335	MS.gene37573	0.829224	5.80E-55	-1.69E-46
MS.gene056335	MS.gene38688	0.804383	2.22E-49	-1.69E-46
MS.gene056335	MS.gene39058	0.816827	4.53E-52	-1.69E-46
MS.gene056335	MS.gene39538	0.840749	7.21E-58	-1.69E-46
MS.gene056335	MS.gene40782	0.801562	8.49E-49	-1.69E-46
MS.gene056335	MS.gene42286	0.836684	8.10E-57	-1.69E-46
MS.gene056335	MS.gene42665	0.802875	4.56E-49	-1.69E-46
MS.gene056335	MS.gene44103	0.815656	8.27E-52	-1.69E-46
MS.gene056335	MS.gene47366	0.820653	6.12E-53	-1.69E-46
MS.gene056335	MS.gene47439	0.802668	5.03E-49	-1.69E-46
MS.gene056335	MS.gene47573	0.817868	2.64E-52	-1.69E-46
MS.gene056335	MS.gene50479	0.801805	7.57E-49	-1.69E-46
MS.gene056335	MS.gene50736	0.820792	5.69E-53	-1.69E-46
MS.gene056335	MS.gene51091	0.807384	5.19E-50	-1.69E-46
MS.gene056335	MS.gene52607	0.831504	1.61E-55	-1.69E-46
MS.gene056335	MS.gene52703	0.874392	7.00E-68	-1.69E-46
MS.gene056335	MS.gene55782	0.80803	3.78E-50	-1.69E-46
MS.gene056335	MS.gene57320	0.806997	6.27E-50	-1.69E-46
MS.gene056335	MS.gene57751	0.81167	6.23E-51	-1.69E-46
MS.gene056335	MS.gene58234	0.813522	2.45E-51	-1.69E-46
MS.gene056335	MS.gene58754	0.800727	1.26E-48	-1.69E-46
MS.gene056335	MS.gene58835	0.807283	5.45E-50	-1.69E-46
MS.gene056335	MS.gene61112	0.840689	7.48E-58	-1.69E-46
MS.gene056335	MS.gene63853	0.819135	1.36E-52	-1.69E-46
MS.gene056335	MS.gene64852	0.801149	1.03E-48	-1.69E-46
MS.gene056335	MS.gene64883	0.815201	1.04E-51	-1.69E-46
MS.gene056335	MS.gene65327	0.817325	3.50E-52	-1.69E-46
MS.gene056335	MS.gene66288	0.803018	4.26E-49	-1.69E-46
MS.gene056335	MS.gene66429	0.80539	1.37E-49	-1.69E-46
MS.gene056335	MS.gene66433	0.816507	5.34E-52	-1.69E-46
MS.gene056335	MS.gene67548	0.800636	1.31E-48	-1.69E-46
MS.gene056335	MS.gene70227	0.80419	2.43E-49	-1.69E-46
MS.gene056335	MS.gene70838	0.845125	4.93E-59	-1.69E-46
MS.gene056335	MS.gene71431	0.802634	5.11E-49	-1.69E-46
MS.gene056335	MS.gene72127	0.838195	3.32E-57	-1.69E-46
MS.gene056335	MS.gene72129	0.830716	2.51E-55	-1.69E-46
MS.gene056335	MS.gene72653	0.807534	4.82E-50	-1.69E-46
MS.gene056335	MS.gene73305	0.804746	1.86E-49	-1.69E-46
MS.gene056335	MS.gene80183	0.809286	2.04E-50	-1.69E-46
MS.gene056335	MS.gene80276	0.807458	5.01E-50	-1.69E-46
MS.gene056335	MS.gene81491	0.802421	5.66E-49	-1.69E-46
MS.gene056335	MS.gene83963	0.802019	6.84E-49	-1.69E-46
MS.gene056335	MS.gene84014	0.830128	3.50E-55	-1.69E-46
MS.gene056335	MS.gene86550	0.825103	5.63E-54	-1.69E-46
MS.gene056335	MS.gene89565	0.818064	2.38E-52	-1.69E-46
MS.gene056335	MS.gene90876	0.849962	2.31E-60	-1.69E-46
MS.gene056335	MS.gene91631	0.850571	1.56E-60	-1.69E-46
MS.gene056335	MS.gene93102	0.861257	1.16E-63	-1.69E-46
MS.gene056335	MS.gene93201	0.82839	9.23E-55	-1.69E-46
MS.gene056335	MS.gene95826	0.803875	2.83E-49	-1.69E-46
MS.gene056335	MS.gene99093	0.824562	7.54E-54	-1.69E-46
MS.gene056335	MS.gene99432	0.821375	4.18E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene055512	MS.gene056335	PPI
MS.gene41679	MS.gene056335	PPI
MS.gene66752	MS.gene056335	PPI
MS.gene58114	MS.gene056335	PPI
MS.gene94307	MS.gene056335	PPI
MS.gene024064	MS.gene056335	PPI
MS.gene78924	MS.gene056335	PPI
MS.gene25357	MS.gene056335	PPI
MS.gene07616	MS.gene056335	PPI
MS.gene056335	MS.gene63118	PPI
MS.gene98552	MS.gene056335	PPI
MS.gene066535	MS.gene056335	PPI
MS.gene038612	MS.gene056335	PPI
MS.gene027907	MS.gene056335	PPI
MS.gene64164	MS.gene056335	PPI
MS.gene28434	MS.gene056335	PPI
MS.gene056335	MS.gene60196	PPI
MS.gene056335	MS.gene29298	PPI
MS.gene014301	MS.gene056335	PPI
MS.gene056335	MS.gene34797	PPI
MS.gene000604	MS.gene056335	PPI
MS.gene24196	MS.gene056335	PPI
MS.gene58116	MS.gene056335	PPI
MS.gene056335	MS.gene46303	PPI
MS.gene030661	MS.gene056335	PPI
MS.gene056335	MS.gene64996	PPI
MS.gene056335	MS.gene26084	PPI
MS.gene056335	MS.gene066960	PPI
MS.gene056335	MS.gene78227	PPI
MS.gene28432	MS.gene056335	PPI
MS.gene79799	MS.gene056335	PPI
MS.gene056335	MS.gene066536	PPI
MS.gene97703	MS.gene056335	PPI
MS.gene059054	MS.gene056335	PPI
MS.gene91631	MS.gene056335	PPI
MS.gene056335	MS.gene03849	PPI
MS.gene60199	MS.gene056335	PPI
MS.gene021887	MS.gene056335	PPI
MS.gene009155	MS.gene056335	PPI
MS.gene52605	MS.gene056335	PPI
MS.gene020313	MS.gene056335	PPI
MS.gene056335	MS.gene35163	PPI
MS.gene070538	MS.gene056335	PPI
MS.gene78323	MS.gene056335	PPI
MS.gene44458	MS.gene056335	PPI
MS.gene056335	MS.gene78224	PPI
MS.gene39538	MS.gene056335	PPI
MS.gene29299	MS.gene056335	PPI
MS.gene042871	MS.gene056335	PPI
MS.gene78922	MS.gene056335	PPI
MS.gene66836	MS.gene056335	PPI
MS.gene033505	MS.gene056335	PPI
MS.gene071851	MS.gene056335	PPI
MS.gene47573	MS.gene056335	PPI
MS.gene50129	MS.gene056335	PPI
MS.gene32513	MS.gene056335	PPI
MS.gene016712	MS.gene056335	PPI
MS.gene056335	MS.gene81646	PPI
MS.gene68716	MS.gene056335	PPI
MS.gene030666	MS.gene056335	PPI
MS.gene056335	MS.gene70990	PPI
MS.gene056335	MS.gene28438	PPI
MS.gene066537	MS.gene056335	PPI
MS.gene97701	MS.gene056335	PPI
MS.gene057458	MS.gene056335	PPI
MS.gene94315	MS.gene056335	PPI
MS.gene056335	MS.gene70261	PPI
MS.gene36496	MS.gene056335	PPI
MS.gene027904	MS.gene056335	PPI
MS.gene29521	MS.gene056335	PPI
MS.gene056335	MS.gene072431	PPI
MS.gene014181	MS.gene056335	PPI
MS.gene018762	MS.gene056335	PPI
MS.gene068178	MS.gene056335	PPI
MS.gene44457	MS.gene056335	PPI
MS.gene056335	MS.gene04748	PPI
MS.gene013163	MS.gene056335	PPI
MS.gene49838	MS.gene056335	PPI
MS.gene32514	MS.gene056335	PPI
MS.gene053230	MS.gene056335	PPI
MS.gene064876	MS.gene056335	PPI
MS.gene66051	MS.gene056335	PPI
MS.gene028296	MS.gene056335	PPI
MS.gene056335	MS.gene05905	PPI
MS.gene04820	MS.gene056335	PPI
MS.gene056335	MS.gene64164	PPI
MS.gene072433	MS.gene056335	PPI
MS.gene65125	MS.gene056335	PPI
MS.gene23886	MS.gene056335	PPI
MS.gene046235	MS.gene056335	PPI
MS.gene056335	MS.gene027909	PPI
MS.gene056335	MS.gene072432	PPI
MS.gene60200	MS.gene056335	PPI
MS.gene015760	MS.gene056335	PPI
MS.gene049111	MS.gene056335	PPI
MS.gene00233	MS.gene056335	PPI
MS.gene70263	MS.gene056335	PPI
MS.gene58285	MS.gene056335	PPI
MS.gene056335	MS.gene030664	PPI
MS.gene68113	MS.gene056335	PPI
MS.gene067742	MS.gene056335	PPI
MS.gene78322	MS.gene056335	PPI
MS.gene056335	MS.gene47284	PPI
MS.gene034255	MS.gene056335	PPI
MS.gene011711	MS.gene056335	PPI
MS.gene056335	MS.gene61193	PPI
MS.gene87898	MS.gene056335	PPI
MS.gene056335	MS.gene069975	PPI
MS.gene056335	MS.gene78324	PPI
MS.gene64998	MS.gene056335	PPI
MS.gene61192	MS.gene056335	PPI
MS.gene056335	MS.gene017431	PPI
MS.gene056335	MS.gene70258	PPI
MS.gene29445	MS.gene056335	PPI
MS.gene26406	MS.gene056335	PPI
MS.gene88233	MS.gene056335	PPI
MS.gene55626	MS.gene056335	PPI
MS.gene89203	MS.gene056335	PPI
MS.gene056335	MS.gene58112	PPI
MS.gene056335	MS.gene011710	PPI
MS.gene056335	MS.gene29300	PPI
MS.gene068179	MS.gene056335	PPI
MS.gene82109	MS.gene056335	PPI
MS.gene063067	MS.gene056335	PPI
MS.gene056335	MS.gene23886	PPI
MS.gene06236	MS.gene056335	PPI
MS.gene95244	MS.gene056335	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056335.t1	MTR_7g059070	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene056335.t1	MTR_5g029820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene056335.t1	MTR_5g029770	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene056335.t1	MTR_4g065990	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene056335.t1	MTR_4g088150	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene056335.t1	MTR_2g035230	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene056335.t1	MTR_8g092720	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene056335.t1	MTR_8g063500	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene056335.t1	MTR_8g103245	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene056335.t1	MTR_8g092820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene056335.t1	MTR_7g013610	100.000	136	0	0	1	136	100	235	1.10e-95	275
MS.gene056335.t1	MTR_4g097175	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene056335.t1	MTR_1g023630	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene056335.t1	MTR_8g061940	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene056335.t1	MTR_8g099430	96.324	136	5	0	1	136	1	136	1.38e-92	264
MS.gene056335.t1	MTR_4g097170	96.324	136	5	0	1	136	42	177	2.29e-91	262
MS.gene056335.t1	MTR_2g082310	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene056335.t1	MTR_2g082340	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene056335.t1	MTR_2g082370	85.616	146	11	1	1	136	1	146	1.74e-82	238
MS.gene056335.t1	MTR_2g082400	84.559	136	19	2	1	135	1	135	6.38e-75	219
MS.gene056335.t1	MTR_7g013600	86.607	112	15	0	25	136	2	113	1.01e-65	195
MS.gene056335.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	6.48e-56	171
MS.gene056335.t1	MTR_1g023630	73.504	117	15	3	1	111	1	107	3.81e-52	160
MS.gene056335.t1	MTR_5g024630	67.241	116	35	2	1	116	1	113	1.63e-45	144
MS.gene056335.t1	MTR_7g023880	58.647	133	41	3	3	134	18	137	5.46e-43	139
MS.gene056335.t1	MTR_5g024530	68.182	110	12	2	19	128	68	154	4.56e-42	137
MS.gene056335.t1	MTR_0197s0100	100.000	66	0	0	1	66	1	66	1.45e-40	132
MS.gene056335.t1	MTR_8g027840	55.102	98	43	1	37	133	28	125	4.83e-30	105
MS.gene056335.t1	MTR_8g105150	53.846	91	10	1	37	127	11	69	1.43e-25	92.4
MS.gene056335.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	4.60e-20	80.9
MS.gene056335.t1	MTR_5g089120	76.190	42	10	0	82	123	6	47	6.07e-18	73.6
MS.gene056335.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.76e-15	66.2
MS.gene056335.t1	MTR_4g128040	75.676	37	9	0	79	115	20	56	3.38e-15	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056335.t1	AT5G65360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene056335.t1	AT5G10390	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene056335.t1	AT5G10400	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene056335.t1	AT3G27360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene056335.t1	AT1G09200	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene056335.t1	AT5G10980	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene056335.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene056335.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene056335.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene056335.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene056335.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene056335.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene056335.t1	AT4G40030	97.059	136	4	0	1	136	29	164	6.76e-93	266
MS.gene056335.t1	AT5G65350	94.853	136	7	0	1	136	1	136	5.09e-91	260
MS.gene056335.t1	AT1G75600	93.382	136	9	0	1	136	1	136	1.20e-89	256
MS.gene056335.t1	AT1G13370	93.382	136	9	0	1	136	1	136	5.32e-89	254
MS.gene056335.t1	AT1G19890	89.781	137	13	1	1	136	1	137	1.03e-83	241
MS.gene056335.t1	AT5G12910	71.111	135	34	2	1	135	1	130	9.51e-67	198
MS.gene056335.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene056335.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene056335.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene056335.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110

Find 34 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCACGAAGTGCGACAGTTC+CGG	0.343802	4.1:+34865342	None:intergenic
TTCTTCACTCCGCCGGTGGC+TGG	0.345560	4.1:+34865382	None:intergenic
TCACGAAGTGCGACAGTTCC+GGG	0.354535	4.1:+34865343	None:intergenic
GGTGGCTGGAGCGGATTTGC+GGG	0.370843	4.1:+34865396	None:intergenic
TTGAATATCCTTGGGCATAA+TGG	0.390549	4.1:+34865117	None:intergenic
CACGAACGAGACGTTGGAAT+GGG	0.414135	4.1:+34865275	None:intergenic
TTATCTCGTTGGACTCTTTG+AGG	0.419676	4.1:-34865176	MS.gene056335:CDS
GAGCAGAAACAGCGCTACTC+TGG	0.427137	4.1:+34865218	None:intergenic
CGCAAATCCGCTCCAGCCAC+CGG	0.436359	4.1:-34865394	MS.gene056335:CDS
CACTCCGCCGGTGGCTGGAG+CGG	0.457833	4.1:+34865387	None:intergenic
CGGTGGCTGGAGCGGATTTG+CGG	0.465165	4.1:+34865395	None:intergenic
GCGAGCGAGTTGAATATCCT+TGG	0.467952	4.1:+34865108	None:intergenic
TCACGAACGAGACGTTGGAA+TGG	0.487442	4.1:+34865274	None:intergenic
CGAGCGAGTTGAATATCCTT+GGG	0.506955	4.1:+34865109	None:intergenic
CGACAGTTCCGGGACGGAAA+CGG	0.513929	4.1:+34865353	None:intergenic
AAGCCTCACCGTTTCCGTCC+CGG	0.527320	4.1:-34865361	MS.gene056335:CDS
AAACAGCGCTACTCTGGAAA+CGG	0.532210	4.1:+34865224	None:intergenic
GCTGCTGAAGCTTATCTCGT+TGG	0.533530	4.1:-34865187	MS.gene056335:CDS
GCTACTCTGGAAACGGAGAT+CGG	0.538527	4.1:+34865231	None:intergenic
CGATTTCACGAACGAGACGT+TGG	0.545134	4.1:+34865269	None:intergenic
CAACTCGCTCGCAGAATCAG+AGG	0.556058	4.1:-34865097	MS.gene056335:CDS
GTGAGGCTTCTTCACTCCGC+CGG	0.560763	4.1:+34865375	None:intergenic
GGCTGGAGCGGATTTGCGGG+CGG	0.566126	4.1:+34865399	None:intergenic
TCTCGTTCGTGAAATCGCTC+AGG	0.574609	4.1:-34865263	MS.gene056335:CDS
AGGCTTCTTCACTCCGCCGG+TGG	0.575034	4.1:+34865378	None:intergenic
TCGCAGAATCAGAGGCGAGA+GGG	0.584947	4.1:-34865089	MS.gene056335:CDS
CTCGCAGAATCAGAGGCGAG+AGG	0.599896	4.1:-34865090	MS.gene056335:CDS
GAGAGTTACCATTATGCCCA+AGG	0.602637	4.1:-34865125	MS.gene056335:CDS
CAAACCGCTCGCAAATCCAC+CGG	0.612149	4.1:-34865457	MS.gene056335:CDS
TCCTCCGGTGGATTTGCGAG+CGG	0.642208	4.1:+34865453	None:intergenic
AAATCCGCTCCAGCCACCGG+CGG	0.651593	4.1:-34865391	MS.gene056335:CDS
ACCGCTCGCAAATCCACCGG+AGG	0.664410	4.1:-34865454	MS.gene056335:CDS
GAAGTGCGACAGTTCCGGGA+CGG	0.679655	4.1:+34865347	None:intergenic
GTTCCGGGACGGAAACGGTG+AGG	0.693353	4.1:+34865358	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGAATATCCTTGGGCATAA+TGG	+	chr4.1:34865442-34865461	None:intergenic	35.0%
!!	TTTGTGGCAAGTTGTTTTCT+TGG	+	chr4.1:34865135-34865154	None:intergenic	35.0%
!!!	CTGTGCTTTTTTGGTATTTG+CGG	+	chr4.1:34865242-34865261	None:intergenic	35.0%
	TTATCTCGTTGGACTCTTTG+AGG	-	chr4.1:34865380-34865399	MS.gene056335:CDS	40.0%
!!!	TGAGAAGCTCTGTGCTTTTT+TGG	+	chr4.1:34865251-34865270	None:intergenic	40.0%
!!!	TTTTCTTGGTGCTTTTCCTC+CGG	+	chr4.1:34865121-34865140	None:intergenic	40.0%
	GAGAGTTACCATTATGCCCA+AGG	-	chr4.1:34865431-34865450	MS.gene056335:CDS	45.0%
!	AAACAGCGCTACTCTGGAAA+CGG	+	chr4.1:34865335-34865354	None:intergenic	45.0%
!	CGAGCGAGTTGAATATCCTT+GGG	+	chr4.1:34865450-34865469	None:intergenic	45.0%
!	GACGTTGGAATGGGAGTTTT+CGG	+	chr4.1:34865275-34865294	None:intergenic	45.0%
	CACGAACGAGACGTTGGAAT+GGG	+	chr4.1:34865284-34865303	None:intergenic	50.0%
	CGATTTCACGAACGAGACGT+TGG	+	chr4.1:34865290-34865309	None:intergenic	50.0%
	GCTGCTGAAGCTTATCTCGT+TGG	-	chr4.1:34865369-34865388	MS.gene056335:CDS	50.0%
	TCACGAACGAGACGTTGGAA+TGG	+	chr4.1:34865285-34865304	None:intergenic	50.0%
	TCTCGTTCGTGAAATCGCTC+AGG	-	chr4.1:34865293-34865312	MS.gene056335:CDS	50.0%
	TTCACGAAGTGCGACAGTTC+CGG	+	chr4.1:34865217-34865236	None:intergenic	50.0%
!	GCGAGCGAGTTGAATATCCT+TGG	+	chr4.1:34865451-34865470	None:intergenic	50.0%
!	GCTACTCTGGAAACGGAGAT+CGG	+	chr4.1:34865328-34865347	None:intergenic	50.0%
	CAAACCGCTCGCAAATCCAC+CGG	-	chr4.1:34865099-34865118	MS.gene056335:CDS	55.0%
	CAACTCGCTCGCAGAATCAG+AGG	-	chr4.1:34865459-34865478	MS.gene056335:CDS	55.0%
	GAGCAGAAACAGCGCTACTC+TGG	+	chr4.1:34865341-34865360	None:intergenic	55.0%
	TCACGAAGTGCGACAGTTCC+GGG	+	chr4.1:34865216-34865235	None:intergenic	55.0%
	TCGCAGAATCAGAGGCGAGA+GGG	-	chr4.1:34865467-34865486	MS.gene056335:CDS	55.0%
!!!	TCTTGGTGCTTTTCCTCCGG+TGG	+	chr4.1:34865118-34865137	None:intergenic	55.0%
	AAGCCTCACCGTTTCCGTCC+CGG	-	chr4.1:34865195-34865214	MS.gene056335:CDS	60.0%
	CGACAGTTCCGGGACGGAAA+CGG	+	chr4.1:34865206-34865225	None:intergenic	60.0%
	CTCGCAGAATCAGAGGCGAG+AGG	-	chr4.1:34865466-34865485	MS.gene056335:CDS	60.0%
	GAAGTGCGACAGTTCCGGGA+CGG	+	chr4.1:34865212-34865231	None:intergenic	60.0%
	GTGAGGCTTCTTCACTCCGC+CGG	+	chr4.1:34865184-34865203	None:intergenic	60.0%
!	GGATTTGCGGGCGGCTTTTG+TGG	+	chr4.1:34865151-34865170	None:intergenic	60.0%
!	TCCTCCGGTGGATTTGCGAG+CGG	+	chr4.1:34865106-34865125	None:intergenic	60.0%
	AAATCCGCTCCAGCCACCGG+CGG	-	chr4.1:34865165-34865184	MS.gene056335:CDS	65.0%
	ACCGCTCGCAAATCCACCGG+AGG	-	chr4.1:34865102-34865121	MS.gene056335:CDS	65.0%
	AGGCTTCTTCACTCCGCCGG+TGG	+	chr4.1:34865181-34865200	None:intergenic	65.0%
	CGCAAATCCGCTCCAGCCAC+CGG	-	chr4.1:34865162-34865181	MS.gene056335:CDS	65.0%
	GTTCCGGGACGGAAACGGTG+AGG	+	chr4.1:34865201-34865220	None:intergenic	65.0%
	TTCTTCACTCCGCCGGTGGC+TGG	+	chr4.1:34865177-34865196	None:intergenic	65.0%
!	CGGTGGCTGGAGCGGATTTG+CGG	+	chr4.1:34865164-34865183	None:intergenic	65.0%
!	GGTGGCTGGAGCGGATTTGC+GGG	+	chr4.1:34865163-34865182	None:intergenic	65.0%
!	GGCTGGAGCGGATTTGCGGG+CGG	+	chr4.1:34865160-34865179	None:intergenic	70.0%
	CACTCCGCCGGTGGCTGGAG+CGG	+	chr4.1:34865172-34865191	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	34865084	34865494	34865084	ID=MS.gene056335
chr4.1	mRNA	34865084	34865494	34865084	ID=MS.gene056335.t1;Parent=MS.gene056335
chr4.1	exon	34865084	34865494	34865084	ID=MS.gene056335.t1.exon1;Parent=MS.gene056335.t1
chr4.1	CDS	34865084	34865494	34865084	ID=cds.MS.gene056335.t1;Parent=MS.gene056335.t1

Gene Sequence

>MS.gene056335

ATGGCGCGTACAAAGCAAACCGCTCGCAAATCCACCGGAGGAAAAGCACCAAGAAAACAACTTGCCACAAAAGCCGCCCGCAAATCCGCTCCAGCCACCGGCGGAGTGAAGAAGCCTCACCGTTTCCGTCCCGGAACTGTCGCACTTCGTGAAATCCGCAAATACCAAAAAAGCACAGAGCTTCTCATCCGAAAACTCCCATTCCAACGTCTCGTTCGTGAAATCGCTCAGGATTTCAAAACCGATCTCCGTTTCCAGAGTAGCGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTCGTTGGACTCTTTGAGGATACGAATCTTTGTGCTATTCATGCTAAGAGAGTTACCATTATGCCCAAGGATATTCAACTCGCTCGCAGAATCAGAGGCGAGAGGGCTTAA

Protein sequence

>MS.gene056335.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA