Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09928.t1 | XP_003611234.1 | 94 | 116 | 7 | 0 | 1 | 116 | 1 | 116 | 6.70E-53 | 216.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09928.t1 | G7KG64 | 94.0 | 116 | 7 | 0 | 1 | 116 | 1 | 116 | 4.8e-53 | 216.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053074 | MS.gene09928 | 0.839045 | 2.00E-57 | -1.69E-46 |
| MS.gene059006 | MS.gene09928 | 0.814375 | 1.59E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09928.t1 | MTR_5g011750 | 93.966 | 116 | 7 | 0 | 1 | 116 | 1 | 116 | 1.15e-76 | 222 |
| MS.gene09928.t1 | MTR_4g102040 | 62.385 | 109 | 36 | 1 | 5 | 113 | 41 | 144 | 8.81e-40 | 130 |
| MS.gene09928.t1 | MTR_2g084805 | 53.125 | 96 | 45 | 0 | 20 | 115 | 51 | 146 | 9.00e-27 | 98.6 |
| MS.gene09928.t1 | MTR_4g055420 | 50.562 | 89 | 44 | 0 | 24 | 112 | 67 | 155 | 8.21e-26 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09928.t1 | AT2G27830 | 52.128 | 94 | 45 | 0 | 19 | 112 | 61 | 154 | 1.05e-30 | 108 |
Find 30 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTGATGACTTATGATA+AGG | 0.419305 | 5.1:-5151121 | MS.gene09928:CDS |
| AACGGAAATTTGTCAGTTTG+AGG | 0.425120 | 5.1:+5151276 | None:intergenic |
| TAGTGAGAAATTGAAGAACT+TGG | 0.435583 | 5.1:-5150753 | MS.gene09928:CDS |
| TAATTACTTTCAAGCGTGAA+TGG | 0.437696 | 5.1:+5151039 | None:intergenic |
| ATGAAATGGTGCAGGTTTGA+AGG | 0.447179 | 5.1:-5150777 | MS.gene09928:intron |
| GGATCGGTGTCCTTCAATAA+TGG | 0.452970 | 5.1:+5151087 | None:intergenic |
| AAGTGAAGTTGGTAGCGGTT+TGG | 0.461676 | 5.1:+5151066 | None:intergenic |
| AGTGAGAAATTGAAGAACTT+GGG | 0.475992 | 5.1:-5150752 | MS.gene09928:CDS |
| TGAATGGAGAGAAGTGAAGT+TGG | 0.482669 | 5.1:+5151055 | None:intergenic |
| TTGGAAGTTCAGATTGAAGT+AGG | 0.484151 | 5.1:+5150701 | None:intergenic |
| GAAGAGAAGGCCATTATTGA+AGG | 0.491115 | 5.1:-5151097 | MS.gene09928:CDS |
| AATTGAAGAACTTGGGGTCA+AGG | 0.499762 | 5.1:-5150745 | MS.gene09928:CDS |
| TGGTCTAAGAGGAGAGTCAT+TGG | 0.503838 | 5.1:+5151239 | None:intergenic |
| AGAAGTTTCGATGGTCTAAG+AGG | 0.521243 | 5.1:+5151228 | None:intergenic |
| GAGAGAAGTGAAGTTGGTAG+CGG | 0.523441 | 5.1:+5151061 | None:intergenic |
| TTTGTCTTTGGCTTCATGGG+TGG | 0.526303 | 5.1:-5151312 | None:intergenic |
| TCAAACTGACAAATTTCCGT+TGG | 0.528724 | 5.1:-5151274 | MS.gene09928:CDS |
| GTCAGTTTGAGGAGTAGAAA+CGG | 0.529246 | 5.1:+5151287 | None:intergenic |
| GTGTCCATCGAGAACACGTT+GGG | 0.537604 | 5.1:-5151198 | MS.gene09928:CDS |
| CAAATTTCCGTTGGATACAA+CGG | 0.549881 | 5.1:-5151265 | MS.gene09928:CDS |
| TTGGTTTCCGTTGTATCCAA+CGG | 0.551596 | 5.1:+5151258 | None:intergenic |
| TACCGTTGGCGAATTAGTGA+AGG | 0.567706 | 5.1:-5151145 | MS.gene09928:CDS |
| GTGAGAAATTGAAGAACTTG+GGG | 0.585031 | 5.1:-5150751 | MS.gene09928:CDS |
| CTTATGATAAGGAGAAGAGA+AGG | 0.588206 | 5.1:-5151110 | MS.gene09928:CDS |
| AAGTTGGTAGCGGTTTGGAT+CGG | 0.590772 | 5.1:+5151071 | None:intergenic |
| ATGTCGCCTGAAAATACCGT+TGG | 0.599343 | 5.1:-5151159 | MS.gene09928:CDS |
| TGCACCCAACGTGTTCTCGA+TGG | 0.600223 | 5.1:+5151194 | None:intergenic |
| ACGTTGAGTAGAAGTTTCGA+TGG | 0.635634 | 5.1:+5151219 | None:intergenic |
| AACCTTCACTAATTCGCCAA+CGG | 0.655659 | 5.1:+5151143 | None:intergenic |
| CGTGTCCATCGAGAACACGT+TGG | 0.692755 | 5.1:-5151199 | MS.gene09928:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATGATATGAAAAAACA+AGG | + | chr5.1:5151038-5151057 | None:intergenic | 15.0% |
| !! | AGGATATAAATTATGAAAAA+AGG | + | chr5.1:5151018-5151037 | None:intergenic | 15.0% |
| !! | GGATATAAATTATGAAAAAA+GGG | + | chr5.1:5151017-5151036 | None:intergenic | 15.0% |
| AGTGAGAAATTGAAGAACTT+GGG | - | chr5.1:5151254-5151273 | MS.gene09928:CDS | 30.0% | |
| ATGAAAAAAGGGAACATTGA+AGG | + | chr5.1:5151006-5151025 | None:intergenic | 30.0% | |
| TAATCAACCACCAAATGAAA+TGG | - | chr5.1:5151215-5151234 | MS.gene09928:CDS | 30.0% | |
| TAATTACTTTCAAGCGTGAA+TGG | + | chr5.1:5150970-5150989 | None:intergenic | 30.0% | |
| TAGTGAGAAATTGAAGAACT+TGG | - | chr5.1:5151253-5151272 | MS.gene09928:CDS | 30.0% | |
| TATACACGATGCATATGATA+AGG | + | chr5.1:5151170-5151189 | None:intergenic | 30.0% | |
| ! | TGCATTGATGACTTATGATA+AGG | - | chr5.1:5150885-5150904 | MS.gene09928:intron | 30.0% |
| AACGGAAATTTGTCAGTTTG+AGG | + | chr5.1:5150733-5150752 | None:intergenic | 35.0% | |
| AAGGGAACATTGAAGGATTA+AGG | + | chr5.1:5150999-5151018 | None:intergenic | 35.0% | |
| CAAATTTCCGTTGGATACAA+CGG | - | chr5.1:5150741-5150760 | MS.gene09928:CDS | 35.0% | |
| CTTATGATAAGGAGAAGAGA+AGG | - | chr5.1:5150896-5150915 | MS.gene09928:intron | 35.0% | |
| GTGAGAAATTGAAGAACTTG+GGG | - | chr5.1:5151255-5151274 | MS.gene09928:CDS | 35.0% | |
| TCAAACTGACAAATTTCCGT+TGG | - | chr5.1:5150732-5150751 | MS.gene09928:CDS | 35.0% | |
| ! | CATTTGGTGGTTGATTAACA+TGG | + | chr5.1:5151212-5151231 | None:intergenic | 35.0% |
| AACCTTCACTAATTCGCCAA+CGG | + | chr5.1:5150866-5150885 | None:intergenic | 40.0% | |
| AATTGAAGAACTTGGGGTCA+AGG | - | chr5.1:5151261-5151280 | MS.gene09928:CDS | 40.0% | |
| ACGTTGAGTAGAAGTTTCGA+TGG | + | chr5.1:5150790-5150809 | None:intergenic | 40.0% | |
| AGAAGTTTCGATGGTCTAAG+AGG | + | chr5.1:5150781-5150800 | None:intergenic | 40.0% | |
| ATGAAATGGTGCAGGTTTGA+AGG | - | chr5.1:5151229-5151248 | MS.gene09928:CDS | 40.0% | |
| CAAACCTGCACCATTTCATT+TGG | + | chr5.1:5151228-5151247 | None:intergenic | 40.0% | |
| GAAGAGAAGGCCATTATTGA+AGG | - | chr5.1:5150909-5150928 | MS.gene09928:intron | 40.0% | |
| GTCAGTTTGAGGAGTAGAAA+CGG | + | chr5.1:5150722-5150741 | None:intergenic | 40.0% | |
| TGAATGGAGAGAAGTGAAGT+TGG | + | chr5.1:5150954-5150973 | None:intergenic | 40.0% | |
| TTGGTTTCCGTTGTATCCAA+CGG | + | chr5.1:5150751-5150770 | None:intergenic | 40.0% | |
| ! | AATTCGCCAACGGTATTTTC+AGG | + | chr5.1:5150856-5150875 | None:intergenic | 40.0% |
| ACCACCAAATGAAATGGTGC+AGG | - | chr5.1:5151221-5151240 | MS.gene09928:CDS | 45.0% | |
| ACCTGCACCATTTCATTTGG+TGG | + | chr5.1:5151225-5151244 | None:intergenic | 45.0% | |
| ATGTCGCCTGAAAATACCGT+TGG | - | chr5.1:5150847-5150866 | MS.gene09928:intron | 45.0% | |
| TACCGTTGGCGAATTAGTGA+AGG | - | chr5.1:5150861-5150880 | MS.gene09928:intron | 45.0% | |
| TGGTCTAAGAGGAGAGTCAT+TGG | + | chr5.1:5150770-5150789 | None:intergenic | 45.0% | |
| ! | GGATCGGTGTCCTTCAATAA+TGG | + | chr5.1:5150922-5150941 | None:intergenic | 45.0% |
| !! | AAGTGAAGTTGGTAGCGGTT+TGG | + | chr5.1:5150943-5150962 | None:intergenic | 45.0% |
| !! | AAGTTGGTAGCGGTTTGGAT+CGG | + | chr5.1:5150938-5150957 | None:intergenic | 45.0% |
| !! | GAGAGAAGTGAAGTTGGTAG+CGG | + | chr5.1:5150948-5150967 | None:intergenic | 45.0% |
| GTGTCCATCGAGAACACGTT+GGG | - | chr5.1:5150808-5150827 | MS.gene09928:intron | 50.0% | |
| CGTGTCCATCGAGAACACGT+TGG | - | chr5.1:5150807-5150826 | MS.gene09928:intron | 55.0% | |
| TGCACCCAACGTGTTCTCGA+TGG | + | chr5.1:5150815-5150834 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 5150709 | 5151319 | 5150709 | ID=MS.gene09928 |
| chr5.1 | mRNA | 5150709 | 5151319 | 5150709 | ID=MS.gene09928.t1;Parent=MS.gene09928 |
| chr5.1 | exon | 5151046 | 5151319 | 5151046 | ID=MS.gene09928.t1.exon1;Parent=MS.gene09928.t1 |
| chr5.1 | CDS | 5151046 | 5151319 | 5151046 | ID=cds.MS.gene09928.t1;Parent=MS.gene09928.t1 |
| chr5.1 | exon | 5150709 | 5150785 | 5150709 | ID=MS.gene09928.t1.exon2;Parent=MS.gene09928.t1 |
| chr5.1 | CDS | 5150709 | 5150785 | 5150709 | ID=cds.MS.gene09928.t1;Parent=MS.gene09928.t1 |
| Gene Sequence |
| Protein sequence |
