Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20013.t1 | XP_003591745.2 | 97.7 | 597 | 14 | 0 | 1 | 597 | 1 | 597 | 0.00E+00 | 1141.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20013.t1 | G7I338 | 97.7 | 597 | 14 | 0 | 1 | 597 | 1 | 597 | 0.0e+00 | 1141.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054892 | MS.gene20013 | 0.809748 | 1.62E-50 | -1.69E-46 |
| MS.gene06061 | MS.gene20013 | 0.812727 | 3.67E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20013.t1 | MTR_1g092540 | 97.655 | 597 | 14 | 0 | 1 | 597 | 1 | 597 | 0.0 | 1207 |
| MS.gene20013.t1 | MTR_3g091180 | 65.506 | 603 | 189 | 6 | 1 | 596 | 1 | 591 | 0.0 | 753 |
| MS.gene20013.t1 | MTR_1g061540 | 39.212 | 584 | 265 | 15 | 5 | 571 | 9 | 519 | 4.16e-107 | 332 |
| MS.gene20013.t1 | MTR_1g073890 | 35.602 | 573 | 302 | 12 | 16 | 577 | 36 | 552 | 2.05e-102 | 322 |
| MS.gene20013.t1 | MTR_7g103490 | 38.276 | 580 | 295 | 10 | 1 | 569 | 5 | 532 | 2.08e-102 | 321 |
| MS.gene20013.t1 | MTR_0318s0020 | 50.000 | 226 | 103 | 6 | 370 | 592 | 351 | 569 | 1.73e-61 | 214 |
| MS.gene20013.t1 | MTR_0318s0020 | 36.727 | 275 | 147 | 5 | 12 | 259 | 114 | 388 | 3.20e-46 | 172 |
| MS.gene20013.t1 | MTR_1g044135 | 60.256 | 156 | 61 | 1 | 423 | 578 | 429 | 583 | 3.82e-55 | 197 |
| MS.gene20013.t1 | MTR_1g044135 | 34.146 | 246 | 143 | 2 | 1 | 229 | 1 | 244 | 2.11e-41 | 159 |
| MS.gene20013.t1 | MTR_6g461910 | 58.642 | 162 | 64 | 3 | 422 | 581 | 342 | 502 | 7.10e-52 | 186 |
| MS.gene20013.t1 | MTR_6g461910 | 39.924 | 263 | 141 | 4 | 19 | 279 | 29 | 276 | 4.50e-49 | 179 |
| MS.gene20013.t1 | MTR_4g021260 | 31.390 | 223 | 145 | 1 | 15 | 229 | 8 | 230 | 2.11e-31 | 128 |
| MS.gene20013.t1 | MTR_4g021260 | 32.661 | 248 | 133 | 9 | 341 | 570 | 242 | 473 | 2.33e-30 | 125 |
| MS.gene20013.t1 | MTR_7g063040 | 29.665 | 209 | 142 | 1 | 26 | 229 | 25 | 233 | 5.42e-26 | 112 |
| MS.gene20013.t1 | MTR_7g063040 | 36.538 | 156 | 90 | 2 | 423 | 570 | 325 | 479 | 1.85e-23 | 104 |
| MS.gene20013.t1 | MTR_1g096970 | 31.707 | 164 | 98 | 2 | 427 | 580 | 263 | 422 | 4.10e-17 | 85.1 |
| MS.gene20013.t1 | MTR_1g096970 | 26.977 | 215 | 137 | 4 | 26 | 229 | 51 | 256 | 7.25e-16 | 81.3 |
| MS.gene20013.t1 | MTR_3g114010 | 32.237 | 152 | 89 | 3 | 433 | 574 | 341 | 488 | 6.00e-14 | 75.5 |
| MS.gene20013.t1 | MTR_3g114010 | 26.316 | 209 | 146 | 3 | 26 | 229 | 122 | 327 | 5.20e-13 | 72.4 |
| MS.gene20013.t1 | MTR_1g025340 | 31.373 | 153 | 90 | 3 | 433 | 574 | 346 | 494 | 1.18e-13 | 74.3 |
| MS.gene20013.t1 | MTR_1g025340 | 26.425 | 193 | 134 | 3 | 23 | 210 | 124 | 313 | 1.99e-13 | 73.6 |
| MS.gene20013.t1 | MTR_3g097240 | 23.529 | 204 | 153 | 2 | 26 | 229 | 141 | 341 | 2.17e-13 | 72.8 |
| MS.gene20013.t1 | MTR_3g097240 | 23.529 | 204 | 153 | 2 | 26 | 229 | 141 | 341 | 2.34e-13 | 72.8 |
| MS.gene20013.t1 | MTR_3g097240 | 23.529 | 204 | 153 | 2 | 26 | 229 | 141 | 341 | 2.99e-13 | 73.2 |
| MS.gene20013.t1 | MTR_3g097240 | 30.147 | 136 | 82 | 3 | 433 | 559 | 357 | 488 | 3.09e-11 | 66.6 |
| MS.gene20013.t1 | MTR_8g095080 | 31.618 | 136 | 80 | 4 | 433 | 559 | 371 | 502 | 7.74e-12 | 68.6 |
| MS.gene20013.t1 | MTR_8g095080 | 23.223 | 211 | 150 | 4 | 26 | 229 | 150 | 355 | 9.12e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20013.t1 | AT5G04550 | 53.177 | 598 | 249 | 11 | 1 | 582 | 1 | 583 | 0.0 | 558 |
| MS.gene20013.t1 | AT5G04550 | 53.177 | 598 | 249 | 11 | 1 | 582 | 1 | 583 | 0.0 | 558 |
| MS.gene20013.t1 | AT5G51670 | 58.861 | 158 | 59 | 2 | 428 | 581 | 308 | 463 | 5.88e-54 | 191 |
| MS.gene20013.t1 | AT5G51670 | 37.209 | 215 | 123 | 1 | 26 | 228 | 37 | 251 | 3.96e-42 | 158 |
| MS.gene20013.t1 | AT5G51670 | 58.861 | 158 | 59 | 2 | 428 | 581 | 308 | 463 | 5.88e-54 | 191 |
| MS.gene20013.t1 | AT5G51670 | 37.209 | 215 | 123 | 1 | 26 | 228 | 37 | 251 | 3.96e-42 | 158 |
| MS.gene20013.t1 | AT3G23160 | 51.479 | 169 | 80 | 2 | 419 | 586 | 345 | 512 | 5.18e-50 | 181 |
| MS.gene20013.t1 | AT3G23160 | 39.614 | 207 | 124 | 1 | 23 | 229 | 40 | 245 | 2.69e-43 | 163 |
| MS.gene20013.t1 | AT1G34320 | 25.472 | 212 | 146 | 3 | 26 | 229 | 155 | 362 | 7.79e-16 | 81.6 |
| MS.gene20013.t1 | AT1G34320 | 32.484 | 157 | 89 | 4 | 430 | 574 | 373 | 524 | 2.62e-15 | 79.7 |
| MS.gene20013.t1 | AT1G34320 | 25.472 | 212 | 146 | 3 | 26 | 229 | 155 | 362 | 7.79e-16 | 81.6 |
| MS.gene20013.t1 | AT1G34320 | 32.484 | 157 | 89 | 4 | 430 | 574 | 373 | 524 | 2.62e-15 | 79.7 |
| MS.gene20013.t1 | AT5G08660 | 34.028 | 144 | 82 | 3 | 435 | 569 | 372 | 511 | 1.03e-15 | 81.3 |
| MS.gene20013.t1 | AT5G08660 | 34.028 | 144 | 82 | 3 | 435 | 569 | 372 | 511 | 1.03e-15 | 81.3 |
| MS.gene20013.t1 | AT5G08660 | 34.028 | 144 | 82 | 3 | 435 | 569 | 372 | 511 | 1.03e-15 | 81.3 |
| MS.gene20013.t1 | AT1G30755 | 29.814 | 161 | 96 | 3 | 433 | 580 | 353 | 509 | 1.62e-12 | 70.9 |
| MS.gene20013.t1 | AT1G30755 | 26.484 | 219 | 147 | 4 | 19 | 229 | 127 | 339 | 2.19e-11 | 67.4 |
| MS.gene20013.t1 | AT1G30755 | 29.814 | 161 | 96 | 3 | 433 | 580 | 353 | 509 | 1.62e-12 | 70.9 |
| MS.gene20013.t1 | AT1G30755 | 26.484 | 219 | 147 | 4 | 19 | 229 | 127 | 339 | 2.19e-11 | 67.4 |
| MS.gene20013.t1 | AT1G30755 | 29.814 | 161 | 96 | 3 | 433 | 580 | 353 | 509 | 1.62e-12 | 70.9 |
| MS.gene20013.t1 | AT1G30755 | 26.484 | 219 | 147 | 4 | 19 | 229 | 127 | 339 | 2.19e-11 | 67.4 |
| MS.gene20013.t1 | AT1G30755 | 26.484 | 219 | 147 | 4 | 19 | 229 | 127 | 339 | 2.62e-11 | 66.6 |
| MS.gene20013.t1 | AT1G30755 | 26.484 | 219 | 147 | 4 | 19 | 229 | 127 | 339 | 2.88e-11 | 66.2 |
| MS.gene20013.t1 | AT1G30755 | 26.484 | 219 | 147 | 4 | 19 | 229 | 127 | 339 | 2.88e-11 | 66.2 |
| MS.gene20013.t1 | AT5G08660 | 39.535 | 86 | 48 | 1 | 435 | 520 | 372 | 453 | 8.06e-11 | 65.1 |
Find 115 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATCTGGTAACATTATTT+TGG | 0.078697 | 1.4:-72596120 | None:intergenic |
| ACGAAGTCAGGCCCGATTTC+GGG | 0.240713 | 1.4:+72596235 | MS.gene20013:CDS |
| CCGCAAATGTGATCAGAATT+TGG | 0.279843 | 1.4:-72596052 | None:intergenic |
| TTCTCAAAATTCTTCAGTCT+TGG | 0.290054 | 1.4:-72595632 | None:intergenic |
| TCAAGAGATGCTTGAGAGTA+TGG | 0.298295 | 1.4:+72595562 | MS.gene20013:CDS |
| GTTTCCAAGGGAATATCATT+AGG | 0.322766 | 1.4:-72596490 | None:intergenic |
| CCTCTATACTTTCCCGAAAT+CGG | 0.325444 | 1.4:-72596247 | None:intergenic |
| AATCATGGTTTCGAAATCTA+TGG | 0.347401 | 1.4:+72595330 | MS.gene20013:CDS |
| GACGAAGTCAGGCCCGATTT+CGG | 0.358468 | 1.4:+72596234 | MS.gene20013:CDS |
| GTACAATGAGTTACATTTCC+AGG | 0.358503 | 1.4:-72596550 | None:intergenic |
| ACAGCCGCAGCCATGGAATA+TGG | 0.370130 | 1.4:-72596778 | None:intergenic |
| AGTCTAATTCTTTGTGGAAC+AGG | 0.376065 | 1.4:+72595909 | MS.gene20013:CDS |
| TCTCAAAATTCTTCAGTCTT+GGG | 0.381346 | 1.4:-72595631 | None:intergenic |
| GGCAATAGTGTAGTAGATAA+TGG | 0.382722 | 1.4:+72597069 | MS.gene20013:CDS |
| GCGAGACGAGATTTCAAATT+CGG | 0.388087 | 1.4:+72595484 | MS.gene20013:CDS |
| AATGTTACCAGATTTGCTTC+AGG | 0.389947 | 1.4:+72596127 | MS.gene20013:CDS |
| CTCAGAGTTATTGCAATCTT+CGG | 0.395849 | 1.4:+72596087 | MS.gene20013:CDS |
| ACTTACAGTCTAATTCTTTG+TGG | 0.399040 | 1.4:+72595903 | MS.gene20013:CDS |
| TTCTGATACTCGATCAAATT+CGG | 0.401763 | 1.4:-72595848 | None:intergenic |
| AAGGGGTCCTGAAGCAAATC+TGG | 0.403486 | 1.4:-72596134 | None:intergenic |
| TTCTTGATTCGCAAAGTAAA+GGG | 0.409376 | 1.4:-72596956 | None:intergenic |
| CCTGTCCCGGATAATGTAAC+AGG | 0.409704 | 1.4:+72596313 | MS.gene20013:CDS |
| TTTCCAAGGGAATATCATTA+GGG | 0.411335 | 1.4:-72596489 | None:intergenic |
| TTCGCGGACAAACGTGTTGT+TGG | 0.427978 | 1.4:+72596927 | MS.gene20013:CDS |
| CTCTATACTTTCCCGAAATC+GGG | 0.428900 | 1.4:-72596246 | None:intergenic |
| AATTCTGAGAAAGCAGTGAT+TGG | 0.430163 | 1.4:+72595377 | MS.gene20013:CDS |
| CCAAATTCTGATCACATTTG+CGG | 0.430479 | 1.4:+72596052 | MS.gene20013:CDS |
| CGGCGAGCATTTCCATCTCT+TGG | 0.439910 | 1.4:-72595775 | None:intergenic |
| GGCGACTCTTCGACGAAGTC+AGG | 0.440304 | 1.4:+72596223 | MS.gene20013:CDS |
| TTTCTTGATTCGCAAAGTAA+AGG | 0.440538 | 1.4:-72596957 | None:intergenic |
| CAATAACATTTGCGTAGTGC+AGG | 0.451579 | 1.4:-72596642 | None:intergenic |
| TGATCTGATTTAGCAGAAGA+AGG | 0.453628 | 1.4:-72596160 | None:intergenic |
| GCAGTCTTTGAGTGATAAAC+AGG | 0.455192 | 1.4:+72595451 | MS.gene20013:CDS |
| CGCAAGCCTAGCCACAGATT+CGG | 0.460018 | 1.4:-72595594 | None:intergenic |
| CTTCAGAGTTTGCTCAAGAT+CGG | 0.460837 | 1.4:-72595795 | None:intergenic |
| AATACAGGTATCTTAGAGAT+TGG | 0.462810 | 1.4:+72597048 | MS.gene20013:CDS |
| TCAGCCTCTCCACACTTGAT+TGG | 0.470012 | 1.4:+72596682 | MS.gene20013:CDS |
| TGATCACATTTGCGGCAGTC+AGG | 0.471870 | 1.4:+72596060 | MS.gene20013:CDS |
| GCAGGTTGCGTGGAAGAAGC+TGG | 0.472548 | 1.4:+72595871 | MS.gene20013:CDS |
| CCTGTTACATTATCCGGGAC+AGG | 0.480680 | 1.4:-72596313 | None:intergenic |
| AGATTTGCTTCAGGACCCCT+TGG | 0.481762 | 1.4:+72596136 | MS.gene20013:CDS |
| TGAAATCTCGTCTCGCAACT+TGG | 0.482654 | 1.4:-72595477 | None:intergenic |
| AGCAGCTGAGCTTTGTTTCG+CGG | 0.483326 | 1.4:+72596911 | MS.gene20013:CDS |
| GATTTAGACCTTCATACTCC+TGG | 0.485555 | 1.4:+72596532 | MS.gene20013:CDS |
| TATGTGAAGGAGCTCAATAC+AGG | 0.487172 | 1.4:+72597033 | MS.gene20013:CDS |
| ATTGAAGAGGTAATAAATGT+TGG | 0.500472 | 1.4:+72596007 | MS.gene20013:CDS |
| GGCTAAAGAAGCGGCACCGA+GGG | 0.505212 | 1.4:-72596620 | None:intergenic |
| CTGCCACACATTGACAAGCT+TGG | 0.505389 | 1.4:-72595432 | None:intergenic |
| GGCGTGTAAGAGCTTCTCTC+AGG | 0.517048 | 1.4:+72596743 | MS.gene20013:CDS |
| CCGATTTCGGGAAAGTATAG+AGG | 0.522748 | 1.4:+72596247 | MS.gene20013:CDS |
| ACTAGCATTCTTCACTCGGT+TGG | 0.524138 | 1.4:+72596202 | MS.gene20013:CDS |
| ATAATCACCGAGCTTCTTGT+CGG | 0.528578 | 1.4:+72596991 | MS.gene20013:CDS |
| ACTTGATTGGTCTCGATGCA+AGG | 0.529764 | 1.4:+72596695 | MS.gene20013:CDS |
| ACGCAAGTCTAGCAGAAGAA+TGG | 0.530524 | 1.4:+72596806 | MS.gene20013:CDS |
| TTCGATATGTTTATTACCCG+TGG | 0.530555 | 1.4:+72595659 | MS.gene20013:CDS |
| CTTAACATGTTGTGAGCAAG+TGG | 0.530855 | 1.4:-72596865 | None:intergenic |
| TGCAAGCTTGTACCAAGAGA+TGG | 0.531229 | 1.4:+72595763 | MS.gene20013:CDS |
| GCAAACTCTGAAGAGAATGA+AGG | 0.531499 | 1.4:+72595805 | MS.gene20013:CDS |
| TTTGATCGAGTATCAGAAGC+AGG | 0.532025 | 1.4:+72595853 | MS.gene20013:CDS |
| TTACATTTCCAGGAGTATGA+AGG | 0.535650 | 1.4:-72596540 | None:intergenic |
| AAACCCTAATGATATTCCCT+TGG | 0.537691 | 1.4:+72596486 | MS.gene20013:CDS |
| CTTTATTTACAATACTCAGT+AGG | 0.546289 | 1.4:+72595966 | MS.gene20013:CDS |
| TAAAACTAGCATTCTTCACT+CGG | 0.549237 | 1.4:+72596198 | MS.gene20013:CDS |
| TTGCTCACAACATGTTAAGA+TGG | 0.550366 | 1.4:+72596869 | MS.gene20013:CDS |
| TTTGTGATTCTGAGTTTCCA+AGG | 0.559767 | 1.4:-72596503 | None:intergenic |
| AGGTTTACAGTGAGTCGGAC+GGG | 0.562547 | 1.4:+72595825 | MS.gene20013:CDS |
| AAGCGATGGTAGCTGAATCA+TGG | 0.567030 | 1.4:+72595315 | None:intergenic |
| CTTGATTGGTCTCGATGCAA+GGG | 0.571162 | 1.4:+72596696 | MS.gene20013:CDS |
| TCCCACTTGTGGTTGTTGAG+TGG | 0.572666 | 1.4:-72596369 | None:intergenic |
| TTGTGATTCTGAGTTTCCAA+GGG | 0.576032 | 1.4:-72596502 | None:intergenic |
| TAAGAGCTTCTCTCAGGACC+AGG | 0.577584 | 1.4:+72596749 | MS.gene20013:CDS |
| GGCCACTCAACAACCACAAG+TGG | 0.577609 | 1.4:+72596367 | MS.gene20013:CDS |
| CCCATACGAATCAAAACCAC+GGG | 0.579429 | 1.4:-72595675 | None:intergenic |
| ATGTGGCCGAATCTGTGGCT+AGG | 0.583300 | 1.4:+72595588 | MS.gene20013:CDS |
| GTCGAAAACAGCCGCAGCCA+TGG | 0.583859 | 1.4:-72596785 | None:intergenic |
| GCACCGAGGGATTCAGATGG+AGG | 0.584223 | 1.4:-72596607 | None:intergenic |
| CAGGCTTAAGCCATATTCCA+TGG | 0.584717 | 1.4:+72596768 | MS.gene20013:CDS |
| CTCAACAACCACAAGTGGGA+AGG | 0.588238 | 1.4:+72596372 | MS.gene20013:CDS |
| CATAGTCGAGCAACTCAAGT+AGG | 0.589346 | 1.4:+72596409 | MS.gene20013:CDS |
| AAGCCTCCATCTGAATCCCT+CGG | 0.594289 | 1.4:+72596604 | MS.gene20013:CDS |
| TTGTCGGTCTGAATTATGTG+TGG | 0.595673 | 1.4:+72597007 | MS.gene20013:CDS |
| ATCTATACAACATGCTGCCG+AGG | 0.599453 | 1.4:+72596722 | MS.gene20013:CDS |
| CATGGAATATGGCTTAAGCC+TGG | 0.599747 | 1.4:-72596767 | None:intergenic |
| GACGAGATTTCAAATTCGGT+CGG | 0.601247 | 1.4:+72595488 | MS.gene20013:CDS |
| GGCACATGTGGCCGAATCTG+TGG | 0.603173 | 1.4:+72595583 | MS.gene20013:CDS |
| TCTATGGAAGACTCAACGTA+AGG | 0.603403 | 1.4:+72595346 | MS.gene20013:CDS |
| TCACATTTGCGGCAGTCAGG+CGG | 0.604695 | 1.4:+72596063 | MS.gene20013:CDS |
| TTGCAAAGAGAACTGAATGT+CGG | 0.605976 | 1.4:-72596577 | None:intergenic |
| AAATTTCTTTATGCATTCAG+CGG | 0.609727 | 1.4:-72597099 | None:intergenic |
| GCGGCAATACAGCCTTTGAA+AGG | 0.610443 | 1.4:-72596433 | None:intergenic |
| ATTCAGACCGACAAGAAGCT+CGG | 0.611368 | 1.4:-72596998 | None:intergenic |
| AATAACATTTGCGTAGTGCA+GGG | 0.614388 | 1.4:-72596641 | None:intergenic |
| TAAGCCATATTCCATGGCTG+CGG | 0.620281 | 1.4:+72596774 | MS.gene20013:CDS |
| AGTATCAGAAGCAGGTTGCG+TGG | 0.624252 | 1.4:+72595861 | MS.gene20013:CDS |
| AAGGTTTACAGTGAGTCGGA+CGG | 0.625325 | 1.4:+72595824 | MS.gene20013:CDS |
| ACCCATACGAATCAAAACCA+CGG | 0.629560 | 1.4:-72595676 | None:intergenic |
| AATGAAGGTTTACAGTGAGT+CGG | 0.629961 | 1.4:+72595820 | MS.gene20013:CDS |
| AGAGAAGCTCTTACACGCCT+CGG | 0.633061 | 1.4:-72596739 | None:intergenic |
| GCGGCACCGAGGGATTCAGA+TGG | 0.634579 | 1.4:-72596610 | None:intergenic |
| ACGGGTAATAAACATATCGA+AGG | 0.648664 | 1.4:-72595657 | None:intergenic |
| TTTAGCAGAAGAAGGACCAA+GGG | 0.649099 | 1.4:-72596152 | None:intergenic |
| GTAGTGCAGGGCTAAAGAAG+CGG | 0.653446 | 1.4:-72596629 | None:intergenic |
| GCTTGAGAGTATGGCACATG+TGG | 0.659391 | 1.4:+72595571 | MS.gene20013:CDS |
| ACTCAAGTAGGACCTTTCAA+AGG | 0.660547 | 1.4:+72596421 | MS.gene20013:CDS |
| GATGACTGAGGAACTATCTG+CGG | 0.666924 | 1.4:-72596452 | None:intergenic |
| ATTTAGCAGAAGAAGGACCA+AGG | 0.666925 | 1.4:-72596153 | None:intergenic |
| GCATCGAGACCAATCAAGTG+TGG | 0.668078 | 1.4:-72596691 | None:intergenic |
| GCCACTCAACAACCACAAGT+GGG | 0.668621 | 1.4:+72596368 | MS.gene20013:CDS |
| GAGACCAATCAAGTGTGGAG+AGG | 0.678449 | 1.4:-72596686 | None:intergenic |
| GGGCTAAAGAAGCGGCACCG+AGG | 0.679045 | 1.4:-72596621 | None:intergenic |
| TGTCCAAGCTTGTCAATGTG+TGG | 0.689258 | 1.4:+72595429 | MS.gene20013:CDS |
| TTATGTGTGGAGATATGTGA+AGG | 0.710243 | 1.4:+72597020 | MS.gene20013:CDS |
| TTAGCAGAAGAAGGACCAAG+GGG | 0.723066 | 1.4:-72596151 | None:intergenic |
| TGAGTGACAGTCGATGACTG+AGG | 0.725297 | 1.4:-72596464 | None:intergenic |
| GGTTGCGTGGAAGAAGCTGG+AGG | 0.742260 | 1.4:+72595874 | MS.gene20013:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAAGAAAATGGATAAAA+AGG | + | chr1.4:72595709-72595728 | MS.gene20013:CDS | 15.0% |
| !! | CAATGACAAAAATATTAGAA+TGG | + | chr1.4:72596836-72596855 | MS.gene20013:CDS | 20.0% |
| !!! | AAATCTGGTAACATTATTTT+GGG | - | chr1.4:72596122-72596141 | None:intergenic | 20.0% |
| !!! | AAGTTTTGGAAGTTTAATTA+TGG | + | chr1.4:72596346-72596365 | MS.gene20013:CDS | 20.0% |
| ! | AAATGGATAAAAAGGTCAAA+AGG | + | chr1.4:72595717-72595736 | MS.gene20013:CDS | 25.0% |
| ! | ATTGAAGAGGTAATAAATGT+TGG | + | chr1.4:72596007-72596026 | MS.gene20013:CDS | 25.0% |
| !! | CAAATCTGGTAACATTATTT+TGG | - | chr1.4:72596123-72596142 | None:intergenic | 25.0% |
| !! | CTTTATTTACAATACTCAGT+AGG | + | chr1.4:72595966-72595985 | MS.gene20013:CDS | 25.0% |
| !! | TTTTTACCTGTTACATTATC+CGG | - | chr1.4:72596322-72596341 | None:intergenic | 25.0% |
| !!! | GGTAATGTCTTTTAAGAAAA+TGG | + | chr1.4:72595700-72595719 | MS.gene20013:CDS | 25.0% |
| AATACAGGTATCTTAGAGAT+TGG | + | chr1.4:72597048-72597067 | MS.gene20013:CDS | 30.0% | |
| AATCATGGTTTCGAAATCTA+TGG | + | chr1.4:72595330-72595349 | MS.gene20013:CDS | 30.0% | |
| ACTTACAGTCTAATTCTTTG+TGG | + | chr1.4:72595903-72595922 | MS.gene20013:CDS | 30.0% | |
| TAAAACTAGCATTCTTCACT+CGG | + | chr1.4:72596198-72596217 | MS.gene20013:CDS | 30.0% | |
| TCTCAAAATTCTTCAGTCTT+GGG | - | chr1.4:72595634-72595653 | None:intergenic | 30.0% | |
| TTCTCAAAATTCTTCAGTCT+TGG | - | chr1.4:72595635-72595654 | None:intergenic | 30.0% | |
| TTCTGATACTCGATCAAATT+CGG | - | chr1.4:72595851-72595870 | None:intergenic | 30.0% | |
| TTTCCAAGGGAATATCATTA+GGG | - | chr1.4:72596492-72596511 | None:intergenic | 30.0% | |
| ! | AGTAGGATCAACAAAGTTTT+TGG | + | chr1.4:72595983-72596002 | MS.gene20013:CDS | 30.0% |
| ! | TTCTTGATTCGCAAAGTAAA+GGG | - | chr1.4:72596959-72596978 | None:intergenic | 30.0% |
| ! | TTTCTTGATTCGCAAAGTAA+AGG | - | chr1.4:72596960-72596979 | None:intergenic | 30.0% |
| ! | TTTTACCTGTTACATTATCC+GGG | - | chr1.4:72596321-72596340 | None:intergenic | 30.0% |
| !! | AAGACTGAAGAATTTTGAGA+AGG | + | chr1.4:72595634-72595653 | MS.gene20013:CDS | 30.0% |
| !!! | CAAAGTTTTTGGAATTGAAG+AGG | + | chr1.4:72595994-72596013 | MS.gene20013:CDS | 30.0% |
| !!! | TAACATTATTTTGGGATGGA+TGG | - | chr1.4:72596114-72596133 | None:intergenic | 30.0% |
| !!! | TAGAGGCATCAATTTTTTCT+CGG | + | chr1.4:72596264-72596283 | MS.gene20013:CDS | 30.0% |
| AAACCCTAATGATATTCCCT+TGG | + | chr1.4:72596486-72596505 | MS.gene20013:CDS | 35.0% | |
| AAGTTCAAAAAAACCTGTCC+CGG | + | chr1.4:72596300-72596319 | MS.gene20013:CDS | 35.0% | |
| AATAACATTTGCGTAGTGCA+GGG | - | chr1.4:72596644-72596663 | None:intergenic | 35.0% | |
| AATGAAGGTTTACAGTGAGT+CGG | + | chr1.4:72595820-72595839 | MS.gene20013:CDS | 35.0% | |
| ACGGGTAATAAACATATCGA+AGG | - | chr1.4:72595660-72595679 | None:intergenic | 35.0% | |
| AGTCTAATTCTTTGTGGAAC+AGG | + | chr1.4:72595909-72595928 | MS.gene20013:CDS | 35.0% | |
| CCAAATTCTGATCACATTTG+CGG | + | chr1.4:72596052-72596071 | MS.gene20013:CDS | 35.0% | |
| CTCAGAGTTATTGCAATCTT+CGG | + | chr1.4:72596087-72596106 | MS.gene20013:CDS | 35.0% | |
| GGATAAAAAGGTCAAAAGGA+TGG | + | chr1.4:72595721-72595740 | MS.gene20013:CDS | 35.0% | |
| GGCAATAGTGTAGTAGATAA+TGG | + | chr1.4:72597069-72597088 | MS.gene20013:CDS | 35.0% | |
| GTACAATGAGTTACATTTCC+AGG | - | chr1.4:72596553-72596572 | None:intergenic | 35.0% | |
| GTTTCCAAGGGAATATCATT+AGG | - | chr1.4:72596493-72596512 | None:intergenic | 35.0% | |
| TGATCTGATTTAGCAGAAGA+AGG | - | chr1.4:72596163-72596182 | None:intergenic | 35.0% | |
| TTATGTGTGGAGATATGTGA+AGG | + | chr1.4:72597020-72597039 | MS.gene20013:CDS | 35.0% | |
| TTCGATATGTTTATTACCCG+TGG | + | chr1.4:72595659-72595678 | MS.gene20013:CDS | 35.0% | |
| TTGCAAAGAGAACTGAATGT+CGG | - | chr1.4:72596580-72596599 | None:intergenic | 35.0% | |
| TTGCTCACAACATGTTAAGA+TGG | + | chr1.4:72596869-72596888 | MS.gene20013:CDS | 35.0% | |
| TTGTGATTCTGAGTTTCCAA+GGG | - | chr1.4:72596505-72596524 | None:intergenic | 35.0% | |
| TTTGTGATTCTGAGTTTCCA+AGG | - | chr1.4:72596506-72596525 | None:intergenic | 35.0% | |
| ! | AATGTTACCAGATTTGCTTC+AGG | + | chr1.4:72596127-72596146 | MS.gene20013:CDS | 35.0% |
| ! | AATTCTGAGAAAGCAGTGAT+TGG | + | chr1.4:72595377-72595396 | MS.gene20013:CDS | 35.0% |
| ! | CAGGTAAAAACAGCAAGTTT+TGG | + | chr1.4:72596332-72596351 | MS.gene20013:CDS | 35.0% |
| ! | TTACATTTCCAGGAGTATGA+AGG | - | chr1.4:72596543-72596562 | None:intergenic | 35.0% |
| !!! | AGAGGCATCAATTTTTTCTC+GGG | + | chr1.4:72596265-72596284 | MS.gene20013:CDS | 35.0% |
| !!! | CTGGTAACATTATTTTGGGA+TGG | - | chr1.4:72596118-72596137 | None:intergenic | 35.0% |
| !!! | TTTTTTGAACTTCTTCCGAG+AGG | - | chr1.4:72596292-72596311 | None:intergenic | 35.0% |
| ACCCATACGAATCAAAACCA+CGG | - | chr1.4:72595679-72595698 | None:intergenic | 40.0% | |
| ACTCAAGTAGGACCTTTCAA+AGG | + | chr1.4:72596421-72596440 | MS.gene20013:CDS | 40.0% | |
| ATAATCACCGAGCTTCTTGT+CGG | + | chr1.4:72596991-72597010 | MS.gene20013:CDS | 40.0% | |
| ATTTAGCAGAAGAAGGACCA+AGG | - | chr1.4:72596156-72596175 | None:intergenic | 40.0% | |
| CAATAACATTTGCGTAGTGC+AGG | - | chr1.4:72596645-72596664 | None:intergenic | 40.0% | |
| CCGCAAATGTGATCAGAATT+TGG | - | chr1.4:72596055-72596074 | None:intergenic | 40.0% | |
| CCTCTATACTTTCCCGAAAT+CGG | - | chr1.4:72596250-72596269 | None:intergenic | 40.0% | |
| CTCTATACTTTCCCGAAATC+GGG | - | chr1.4:72596249-72596268 | None:intergenic | 40.0% | |
| CTTAACATGTTGTGAGCAAG+TGG | - | chr1.4:72596868-72596887 | None:intergenic | 40.0% | |
| GACGAGATTTCAAATTCGGT+CGG | + | chr1.4:72595488-72595507 | MS.gene20013:CDS | 40.0% | |
| GATTTAGACCTTCATACTCC+TGG | + | chr1.4:72596532-72596551 | MS.gene20013:CDS | 40.0% | |
| GCAGTCTTTGAGTGATAAAC+AGG | + | chr1.4:72595451-72595470 | MS.gene20013:CDS | 40.0% | |
| GCGAGACGAGATTTCAAATT+CGG | + | chr1.4:72595484-72595503 | MS.gene20013:CDS | 40.0% | |
| TATGTGAAGGAGCTCAATAC+AGG | + | chr1.4:72597033-72597052 | MS.gene20013:CDS | 40.0% | |
| TCAAGAGATGCTTGAGAGTA+TGG | + | chr1.4:72595562-72595581 | MS.gene20013:CDS | 40.0% | |
| TCTATGGAAGACTCAACGTA+AGG | + | chr1.4:72595346-72595365 | MS.gene20013:CDS | 40.0% | |
| TTGTCGGTCTGAATTATGTG+TGG | + | chr1.4:72597007-72597026 | MS.gene20013:CDS | 40.0% | |
| TTTAGCAGAAGAAGGACCAA+GGG | - | chr1.4:72596155-72596174 | None:intergenic | 40.0% | |
| TTTGATCGAGTATCAGAAGC+AGG | + | chr1.4:72595853-72595872 | MS.gene20013:CDS | 40.0% | |
| ! | CTTCAGAGTTTGCTCAAGAT+CGG | - | chr1.4:72595798-72595817 | None:intergenic | 40.0% |
| ! | GCAAACTCTGAAGAGAATGA+AGG | + | chr1.4:72595805-72595824 | MS.gene20013:CDS | 40.0% |
| !! | GTTATTTTCCTTCCCACTTG+TGG | - | chr1.4:72596383-72596402 | None:intergenic | 40.0% |
| !!! | AATTTTTTCTCGGGACCTCT+CGG | + | chr1.4:72596274-72596293 | MS.gene20013:CDS | 40.0% |
| !!! | TGGTTTTGATTCGTATGGGT+GGG | + | chr1.4:72595679-72595698 | MS.gene20013:CDS | 40.0% |
| AAGGTTTACAGTGAGTCGGA+CGG | + | chr1.4:72595824-72595843 | MS.gene20013:CDS | 45.0% | |
| ACGCAAGTCTAGCAGAAGAA+TGG | + | chr1.4:72596806-72596825 | MS.gene20013:CDS | 45.0% | |
| ACTAGCATTCTTCACTCGGT+TGG | + | chr1.4:72596202-72596221 | MS.gene20013:CDS | 45.0% | |
| ATCTATACAACATGCTGCCG+AGG | + | chr1.4:72596722-72596741 | MS.gene20013:CDS | 45.0% | |
| ATTCAGACCGACAAGAAGCT+CGG | - | chr1.4:72597001-72597020 | None:intergenic | 45.0% | |
| CAGGCTTAAGCCATATTCCA+TGG | + | chr1.4:72596768-72596787 | MS.gene20013:CDS | 45.0% | |
| CATAGTCGAGCAACTCAAGT+AGG | + | chr1.4:72596409-72596428 | MS.gene20013:CDS | 45.0% | |
| CATGGAATATGGCTTAAGCC+TGG | - | chr1.4:72596770-72596789 | None:intergenic | 45.0% | |
| CCCATACGAATCAAAACCAC+GGG | - | chr1.4:72595678-72595697 | None:intergenic | 45.0% | |
| GATGACTGAGGAACTATCTG+CGG | - | chr1.4:72596455-72596474 | None:intergenic | 45.0% | |
| TAAGCCATATTCCATGGCTG+CGG | + | chr1.4:72596774-72596793 | MS.gene20013:CDS | 45.0% | |
| TGAAATCTCGTCTCGCAACT+TGG | - | chr1.4:72595480-72595499 | None:intergenic | 45.0% | |
| TGCAAGCTTGTACCAAGAGA+TGG | + | chr1.4:72595763-72595782 | MS.gene20013:CDS | 45.0% | |
| TGTCCAAGCTTGTCAATGTG+TGG | + | chr1.4:72595429-72595448 | MS.gene20013:CDS | 45.0% | |
| TTAGCAGAAGAAGGACCAAG+GGG | - | chr1.4:72596154-72596173 | None:intergenic | 45.0% | |
| ! | CCGATTTCGGGAAAGTATAG+AGG | + | chr1.4:72596247-72596266 | MS.gene20013:CDS | 45.0% |
| !! | ACTTGATTGGTCTCGATGCA+AGG | + | chr1.4:72596695-72596714 | MS.gene20013:CDS | 45.0% |
| !! | CTTGATTGGTCTCGATGCAA+GGG | + | chr1.4:72596696-72596715 | MS.gene20013:CDS | 45.0% |
| !!! | ACCCGTGGTTTTGATTCGTA+TGG | + | chr1.4:72595674-72595693 | MS.gene20013:CDS | 45.0% |
| !!! | CCCGTGGTTTTGATTCGTAT+GGG | + | chr1.4:72595675-72595694 | MS.gene20013:CDS | 45.0% |
| !!! | GTGGTTTTGATTCGTATGGG+TGG | + | chr1.4:72595678-72595697 | MS.gene20013:CDS | 45.0% |
| AAGCCTCCATCTGAATCCCT+CGG | + | chr1.4:72596604-72596623 | MS.gene20013:CDS | 50.0% | |
| AAGGGGTCCTGAAGCAAATC+TGG | - | chr1.4:72596137-72596156 | None:intergenic | 50.0% | |
| AGAGAAGCTCTTACACGCCT+CGG | - | chr1.4:72596742-72596761 | None:intergenic | 50.0% | |
| AGGTTTACAGTGAGTCGGAC+GGG | + | chr1.4:72595825-72595844 | MS.gene20013:CDS | 50.0% | |
| AGTATCAGAAGCAGGTTGCG+TGG | + | chr1.4:72595861-72595880 | MS.gene20013:CDS | 50.0% | |
| CCTGTCCCGGATAATGTAAC+AGG | + | chr1.4:72596313-72596332 | MS.gene20013:CDS | 50.0% | |
| CCTGTTACATTATCCGGGAC+AGG | - | chr1.4:72596316-72596335 | None:intergenic | 50.0% | |
| CTCAACAACCACAAGTGGGA+AGG | + | chr1.4:72596372-72596391 | MS.gene20013:CDS | 50.0% | |
| CTGCCACACATTGACAAGCT+TGG | - | chr1.4:72595435-72595454 | None:intergenic | 50.0% | |
| GAGACCAATCAAGTGTGGAG+AGG | - | chr1.4:72596689-72596708 | None:intergenic | 50.0% | |
| GCATCGAGACCAATCAAGTG+TGG | - | chr1.4:72596694-72596713 | None:intergenic | 50.0% | |
| GCCACTCAACAACCACAAGT+GGG | + | chr1.4:72596368-72596387 | MS.gene20013:CDS | 50.0% | |
| GCGGCAATACAGCCTTTGAA+AGG | - | chr1.4:72596436-72596455 | None:intergenic | 50.0% | |
| GTAGTGCAGGGCTAAAGAAG+CGG | - | chr1.4:72596632-72596651 | None:intergenic | 50.0% | |
| TAAGAGCTTCTCTCAGGACC+AGG | + | chr1.4:72596749-72596768 | MS.gene20013:CDS | 50.0% | |
| TCAGCCTCTCCACACTTGAT+TGG | + | chr1.4:72596682-72596701 | MS.gene20013:CDS | 50.0% | |
| TCCCACTTGTGGTTGTTGAG+TGG | - | chr1.4:72596372-72596391 | None:intergenic | 50.0% | |
| TGAGTGACAGTCGATGACTG+AGG | - | chr1.4:72596467-72596486 | None:intergenic | 50.0% | |
| TGATCACATTTGCGGCAGTC+AGG | + | chr1.4:72596060-72596079 | MS.gene20013:CDS | 50.0% | |
| TTCGCGGACAAACGTGTTGT+TGG | + | chr1.4:72596927-72596946 | MS.gene20013:CDS | 50.0% | |
| ! | AGATTTGCTTCAGGACCCCT+TGG | + | chr1.4:72596136-72596155 | MS.gene20013:CDS | 50.0% |
| ! | GCTTGAGAGTATGGCACATG+TGG | + | chr1.4:72595571-72595590 | MS.gene20013:CDS | 50.0% |
| !! | AGCAGCTGAGCTTTGTTTCG+CGG | + | chr1.4:72596911-72596930 | MS.gene20013:CDS | 50.0% |
| ACAGCCGCAGCCATGGAATA+TGG | - | chr1.4:72596781-72596800 | None:intergenic | 55.0% | |
| ACGAAGTCAGGCCCGATTTC+GGG | + | chr1.4:72596235-72596254 | MS.gene20013:CDS | 55.0% | |
| ATGTGGCCGAATCTGTGGCT+AGG | + | chr1.4:72595588-72595607 | MS.gene20013:CDS | 55.0% | |
| CGCAAGCCTAGCCACAGATT+CGG | - | chr1.4:72595597-72595616 | None:intergenic | 55.0% | |
| CGGCGAGCATTTCCATCTCT+TGG | - | chr1.4:72595778-72595797 | None:intergenic | 55.0% | |
| GACGAAGTCAGGCCCGATTT+CGG | + | chr1.4:72596234-72596253 | MS.gene20013:CDS | 55.0% | |
| GGCCACTCAACAACCACAAG+TGG | + | chr1.4:72596367-72596386 | MS.gene20013:CDS | 55.0% | |
| GGCGTGTAAGAGCTTCTCTC+AGG | + | chr1.4:72596743-72596762 | MS.gene20013:CDS | 55.0% | |
| TCACATTTGCGGCAGTCAGG+CGG | + | chr1.4:72596063-72596082 | MS.gene20013:CDS | 55.0% | |
| GCACCGAGGGATTCAGATGG+AGG | - | chr1.4:72596610-72596629 | None:intergenic | 60.0% | |
| GCAGGTTGCGTGGAAGAAGC+TGG | + | chr1.4:72595871-72595890 | MS.gene20013:CDS | 60.0% | |
| GGCACATGTGGCCGAATCTG+TGG | + | chr1.4:72595583-72595602 | MS.gene20013:CDS | 60.0% | |
| GGCGACTCTTCGACGAAGTC+AGG | + | chr1.4:72596223-72596242 | MS.gene20013:CDS | 60.0% | |
| GGTTGCGTGGAAGAAGCTGG+AGG | + | chr1.4:72595874-72595893 | MS.gene20013:CDS | 60.0% | |
| GTCGAAAACAGCCGCAGCCA+TGG | - | chr1.4:72596788-72596807 | None:intergenic | 60.0% | |
| ! | GGCTAAAGAAGCGGCACCGA+GGG | - | chr1.4:72596623-72596642 | None:intergenic | 60.0% |
| GGGCTAAAGAAGCGGCACCG+AGG | - | chr1.4:72596624-72596643 | None:intergenic | 65.0% | |
| ! | GCGGCACCGAGGGATTCAGA+TGG | - | chr1.4:72596613-72596632 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 72595320 | 72597113 | 72595320 | ID=MS.gene20013 |
| chr1.4 | mRNA | 72595320 | 72597113 | 72595320 | ID=MS.gene20013.t1;Parent=MS.gene20013 |
| chr1.4 | exon | 72595320 | 72597113 | 72595320 | ID=MS.gene20013.t1.exon1;Parent=MS.gene20013.t1 |
| chr1.4 | CDS | 72595320 | 72597113 | 72595320 | ID=cds.MS.gene20013.t1;Parent=MS.gene20013.t1 |
| Gene Sequence |
| Protein sequence |
