AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20141

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20141.t1 MTR_3g084630 95.357 280 13 0 4 283 3 282 0.0 540
MS.gene20141.t1 MTR_3g084620 95.324 278 13 0 6 283 5 282 0.0 536
MS.gene20141.t1 MTR_8g102220 89.716 282 17 2 1 282 1 270 0.0 513
MS.gene20141.t1 MTR_8g081040 86.989 269 33 2 11 278 14 281 4.97e-179 494
MS.gene20141.t1 MTR_4g128410 71.898 274 69 2 15 281 11 283 4.46e-144 406
MS.gene20141.t1 MTR_7g093530 72.222 270 69 1 15 278 10 279 1.21e-141 400
MS.gene20141.t1 MTR_4g128570 68.364 275 78 2 8 281 9 275 1.31e-138 392
MS.gene20141.t1 MTR_4g128580 71.111 270 68 1 22 281 19 288 2.27e-138 392
MS.gene20141.t1 MTR_4g128400 65.568 273 88 1 12 278 11 283 2.65e-133 379
MS.gene20141.t1 MTR_2g038860 65.468 278 85 1 16 282 13 290 2.29e-132 377
MS.gene20141.t1 MTR_2g038705 65.468 278 85 1 16 282 13 290 2.29e-132 377
MS.gene20141.t1 MTR_4g126920 67.300 263 79 1 23 278 19 281 5.16e-130 370
MS.gene20141.t1 MTR_4g128590 65.157 287 70 4 9 281 8 278 8.66e-129 367
MS.gene20141.t1 MTR_7g084770 53.357 283 120 3 11 282 10 291 1.13e-105 309
MS.gene20141.t1 MTR_5g091730 52.963 270 126 1 12 280 11 280 1.26e-104 306
MS.gene20141.t1 MTR_7g084760 52.297 283 123 3 11 282 10 291 1.10e-103 304
MS.gene20141.t1 MTR_6g033055 52.055 292 122 4 8 282 1 291 5.96e-103 302
MS.gene20141.t1 MTR_7g084750 51.399 286 127 3 8 282 7 291 9.13e-103 301
MS.gene20141.t1 MTR_6g033085 51.736 288 127 3 6 282 5 291 1.74e-102 301
MS.gene20141.t1 MTR_8g102910 52.128 282 122 5 12 283 6 284 7.62e-102 299
MS.gene20141.t1 MTR_6g088320 50.368 272 123 2 22 283 19 288 1.51e-96 286
MS.gene20141.t1 MTR_3g089540 50.575 261 122 2 26 281 27 285 3.54e-95 282
MS.gene20141.t1 MTR_2g089140 48.966 290 128 6 7 280 3 288 4.63e-94 280
MS.gene20141.t1 MTR_4g045853 47.959 294 131 6 1 278 1 288 7.46e-93 276
MS.gene20141.t1 MTR_4g097640 48.727 275 132 4 11 278 15 287 1.76e-92 275
MS.gene20141.t1 MTR_4g097670 48.727 275 132 4 11 278 15 287 1.76e-92 275
MS.gene20141.t1 MTR_4g097700 48.689 267 129 3 19 278 59 324 4.02e-91 273
MS.gene20141.t1 MTR_1g105900 46.350 274 137 2 18 283 25 296 2.37e-90 270
MS.gene20141.t1 MTR_2g089140 52.655 226 91 4 70 280 1 225 4.17e-83 249
MS.gene20141.t1 MTR_1g105495 44.599 287 142 4 11 281 12 297 1.91e-82 250
MS.gene20141.t1 MTR_8g013055 45.000 260 128 4 29 278 31 285 7.42e-78 238
MS.gene20141.t1 MTR_7g102260 41.288 264 138 6 26 278 34 291 4.48e-63 200
MS.gene20141.t1 MTR_7g056090 40.602 266 143 6 12 266 12 273 1.94e-62 198
MS.gene20141.t1 MTR_1g063020 39.773 264 142 6 26 278 37 294 4.07e-59 190
MS.gene20141.t1 MTR_4g057450 41.603 262 136 6 28 278 49 304 6.97e-59 190
MS.gene20141.t1 MTR_2g095800 39.924 263 139 6 19 269 46 301 5.76e-58 188
MS.gene20141.t1 MTR_2g049130 39.344 244 136 5 29 264 29 268 7.76e-56 181
MS.gene20141.t1 MTR_1g033420 37.548 261 150 6 29 278 33 291 1.07e-50 169
MS.gene20141.t1 MTR_2g049030 35.317 252 149 6 29 272 30 275 3.25e-50 167
MS.gene20141.t1 MTR_8g064180 34.962 266 150 8 33 279 50 311 4.54e-50 167
MS.gene20141.t1 MTR_5g029100 37.402 254 138 8 41 278 57 305 5.85e-49 166
MS.gene20141.t1 MTR_5g029100 34.749 259 145 9 42 278 18 274 1.71e-45 156
MS.gene20141.t1 MTR_8g090100 34.601 263 157 5 29 278 21 281 6.67e-44 152
MS.gene20141.t1 MTR_8g479340 30.808 198 56 8 98 281 8 138 2.60e-16 74.3
MS.gene20141.t1 MTR_8g032640 32.558 172 108 5 51 220 33 198 3.33e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20141.t1 AT3G23730 73.077 286 66 2 8 282 5 290 8.38e-165 459
MS.gene20141.t1 AT4G14130 74.113 282 66 2 8 282 7 288 3.31e-163 454
MS.gene20141.t1 AT4G25810 65.818 275 86 1 12 278 9 283 4.39e-134 381
MS.gene20141.t1 AT4G30270 64.576 271 82 2 11 281 12 268 5.56e-129 367
MS.gene20141.t1 AT5G57550 65.759 257 85 1 26 279 27 283 1.00e-128 367
MS.gene20141.t1 AT5G57560 63.670 267 88 1 21 278 15 281 2.18e-128 366
MS.gene20141.t1 AT5G57530 63.396 265 81 2 26 279 24 283 1.88e-125 359
MS.gene20141.t1 AT5G57540 63.019 265 82 3 26 279 23 282 5.49e-125 358
MS.gene20141.t1 AT4G25820 63.534 266 89 2 21 279 22 286 7.66e-123 352
MS.gene20141.t1 AT5G48070 57.295 281 117 2 1 278 1 281 5.55e-120 345
MS.gene20141.t1 AT2G18800 57.857 280 99 2 18 278 17 296 2.71e-119 344
MS.gene20141.t1 AT4G30280 56.228 281 120 2 1 278 1 281 1.39e-109 318
MS.gene20141.t1 AT4G30290 58.889 270 108 2 12 278 7 276 1.68e-109 318
MS.gene20141.t1 AT1G65310 60.156 256 99 2 26 278 48 303 6.93e-108 315
MS.gene20141.t1 AT1G65310 60.156 256 99 2 26 278 26 281 8.36e-108 314
MS.gene20141.t1 AT5G13870 52.669 281 123 3 11 282 12 291 4.45e-102 300
MS.gene20141.t1 AT5G13870 52.669 281 123 3 11 282 12 291 4.45e-102 300
MS.gene20141.t1 AT5G13870 52.669 281 123 3 11 282 12 291 4.45e-102 300
MS.gene20141.t1 AT2G06850 51.579 285 124 3 11 282 11 294 1.67e-99 293
MS.gene20141.t1 AT4G03210 48.936 282 130 5 11 282 11 288 3.87e-95 282
MS.gene20141.t1 AT4G28850 48.601 286 131 5 11 283 10 292 2.11e-92 275
MS.gene20141.t1 AT2G06850 56.710 231 92 2 59 282 5 234 2.74e-92 273
MS.gene20141.t1 AT5G65730 49.627 268 121 5 22 279 28 291 3.24e-91 272
MS.gene20141.t1 AT2G14620 44.792 288 144 2 11 283 12 299 6.47e-90 269
MS.gene20141.t1 AT4G37800 48.659 261 122 4 27 278 32 289 8.61e-88 263
MS.gene20141.t1 AT4G03210 52.381 231 100 3 61 282 31 260 9.32e-87 260
MS.gene20141.t1 AT1G11545 44.108 297 149 5 1 281 7 302 8.52e-86 259
MS.gene20141.t1 AT2G14620 47.436 234 115 1 58 283 16 249 1.64e-80 243
MS.gene20141.t1 AT2G14620 48.430 223 107 1 69 283 16 238 2.80e-78 238
MS.gene20141.t1 AT4G13090 42.268 291 147 5 8 279 1 289 4.68e-75 231
MS.gene20141.t1 AT3G25050 39.362 282 153 4 6 278 17 289 2.10e-74 229
MS.gene20141.t1 AT4G13080 44.238 269 131 7 22 279 32 292 5.43e-72 223
MS.gene20141.t1 AT2G36870 42.481 266 136 6 26 280 40 299 3.83e-63 201
MS.gene20141.t1 AT3G44990 40.152 264 141 7 26 278 36 293 1.86e-56 183
MS.gene20141.t1 AT1G10550 35.662 272 140 8 33 278 47 309 7.17e-52 172
MS.gene20141.t1 AT1G32170 38.272 243 132 6 51 278 54 293 9.52e-52 173
MS.gene20141.t1 AT1G14720 36.397 272 154 6 29 282 31 301 1.15e-51 172
MS.gene20141.t1 AT2G01850 31.438 299 180 6 8 282 4 301 9.12e-49 165
MS.gene20141.t1 AT4G18990 34.601 263 140 7 51 283 62 322 3.26e-44 154
MS.gene20141.t1 AT3G48580 33.462 260 151 8 23 278 32 273 3.73e-43 149
MS.gene20141.t1 AT4G18990 33.755 237 121 8 78 283 17 248 1.03e-36 132
MS.gene20141.t1 AT3G48580 31.556 225 137 6 58 278 3 214 3.65e-36 129
MS.gene20141.t1 AT2G36870 43.262 141 70 3 26 158 40 178 6.97e-31 114
MS.gene20141.t1 AT3G48580 30.566 265 132 11 23 278 32 253 1.14e-27 107

Find 62 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTCATTGGTTGGTTCTT+TGG 0.181799 4.4:+3638102 None:intergenic
ACCGAATAAGTACTCTTTCT+TGG 0.193166 4.4:+3638693 None:intergenic
GCATTCTTGAAGATCCTTAT+TGG 0.229939 4.4:+3638147 None:intergenic
TTCTCATTGGTTGGTTCTTT+GGG 0.250243 4.4:+3638103 None:intergenic
GATATATAAATACAGATTCT+TGG 0.289430 4.4:-3638181 MS.gene20141:intron
AATTGATATGCAACTCAAAT+TGG 0.296036 4.4:-3638668 MS.gene20141:CDS
CATTCTTGAAGATCCTTATT+GGG 0.315649 4.4:+3638148 None:intergenic
GTCTCTGGAACGCCGATGAT+TGG 0.334816 4.4:-3638071 MS.gene20141:CDS
TCCACACTTACTCTATCATT+TGG 0.354596 4.4:-3638317 MS.gene20141:CDS
GATTACTTACATAATATGTT+GGG 0.356492 4.4:+3638290 None:intergenic
CTGCTGAGTGTAGGCGTTCA+AGG 0.362843 4.4:-3637816 MS.gene20141:CDS
TGGTTGACAACATCCCAATA+AGG 0.367483 4.4:-3638161 MS.gene20141:CDS
TTGCGGTAGTAAGCTCTAAA+TGG 0.387620 4.4:+3638006 None:intergenic
AGACTTGAATATATTCTCAT+TGG 0.390012 4.4:+3638090 None:intergenic
TTCAAGAATGCTGAATCTAT+TGG 0.397240 4.4:-3638135 MS.gene20141:CDS
TGAGAATATATTCAAGTCTC+TGG 0.397799 4.4:-3638086 MS.gene20141:CDS
TGATTACTTACATAATATGT+TGG 0.399429 4.4:+3638289 None:intergenic
AGCAACGAGCTTGATGCTTA+TGG 0.410097 4.4:-3637922 MS.gene20141:CDS
CGCATGAACAATATGCTGAT+TGG 0.411159 4.4:-3638868 MS.gene20141:CDS
CTCGACAAAGTCTCCGGCTC+TGG 0.411568 4.4:-3638724 MS.gene20141:CDS
TCCAAGAAAGAGTACTTATT+CGG 0.435562 4.4:-3638694 MS.gene20141:CDS
ATGCAACTCAAATTGGTTGC+TGG 0.439162 4.4:-3638661 MS.gene20141:CDS
TTGGTTGCTGGAAACTCTGC+TGG 0.443966 4.4:-3638649 MS.gene20141:CDS
TCTCTGGAACGCCGATGATT+GGG 0.447932 4.4:-3638070 MS.gene20141:CDS
AGAGTAAGTGTGGAAGTTCT+TGG 0.454863 4.4:+3638326 None:intergenic
GTAAGCTCTAAATGGTGCTT+TGG 0.462686 4.4:+3638014 None:intergenic
CCCATGTTAGATCAAAATCT+TGG 0.466463 4.4:+3638797 None:intergenic
TGCAGAGCATGTAGCTATCA+AGG 0.475147 4.4:+3638828 None:intergenic
TTGGGCTACCAGAGGTGGTT+TGG 0.478609 4.4:-3638052 MS.gene20141:CDS
TTGAATATATTCTCATTGGT+TGG 0.480043 4.4:+3638094 None:intergenic
ATAAGTACTCTTTCTTGGAC+TGG 0.482666 4.4:+3638698 None:intergenic
CTTTCTCTCGACAAAGTCTC+CGG 0.484184 4.4:-3638730 MS.gene20141:CDS
ACGTTTGTGTGGAGAATGTA+AGG 0.490530 4.4:+3638405 None:intergenic
ATTGGTTGGTTCTTTGGGAA+AGG 0.493174 4.4:+3638108 None:intergenic
CTTGGACTGGAAGCCAGAGC+CGG 0.496315 4.4:+3638711 None:intergenic
GAGAACAACAATTCTACCTC+TGG 0.497697 4.4:-3638359 MS.gene20141:CDS
AAATCAATTTCATCGTGAGT+TGG 0.537756 4.4:+3638456 None:intergenic
ATGGTAGAAGAAGATTGAGA+TGG 0.542822 4.4:-3637903 MS.gene20141:CDS
GTGGTTTGGTGAAAACTGAT+TGG 0.548484 4.4:-3638038 MS.gene20141:CDS
GAGTTCTTGGGAAACGTTAG+CGG 0.548565 4.4:-3638432 MS.gene20141:CDS
GATGATTGGGCTACCAGAGG+TGG 0.556788 4.4:-3638057 MS.gene20141:CDS
CTTGAACGCCTACACTCAGC+AGG 0.559110 4.4:+3637817 None:intergenic
GTTCAGTAGCTTTGAAATTG+CGG 0.561695 4.4:+3637989 None:intergenic
ATTACTTACATAATATGTTG+GGG 0.569004 4.4:+3638291 None:intergenic
ACCTCTGGTAGCCCAATCAT+CGG 0.571587 4.4:+3638059 None:intergenic
AAGGTATTCCTGCTGAGTGT+AGG 0.574136 4.4:-3637825 MS.gene20141:CDS
TCCAAATGATAGAGTAAGTG+TGG 0.575302 4.4:+3638316 None:intergenic
TGGTAGAAGAAGATTGAGAT+GGG 0.586469 4.4:-3637902 MS.gene20141:CDS
CACCGTGCTAAGATATTCAA+CGG 0.594582 4.4:-3638769 MS.gene20141:CDS
CACTCAGCAGGAATACCTTG+TGG 0.608976 4.4:+3637829 None:intergenic
TGCAGAATCAGAGAATGAAG+TGG 0.609785 4.4:+3637951 None:intergenic
ATGGTGCAGTTGTCATCACA+AGG 0.628374 4.4:-3638480 MS.gene20141:intron
GTAAGTGTGGAAGTTCTTGG+TGG 0.628497 4.4:+3638329 None:intergenic
CTTGAGAAAACACGTTTGTG+TGG 0.633605 4.4:+3638394 None:intergenic
CACCGTTGAATATCTTAGCA+CGG 0.635606 4.4:+3638767 None:intergenic
TAAGTGTGGAAGTTCTTGGT+GGG 0.638179 4.4:+3638330 None:intergenic
GCCGATGATTGGGCTACCAG+AGG 0.643017 4.4:-3638060 MS.gene20141:CDS
GATCTTAAGCGATTTCCACA+AGG 0.665367 4.4:-3637844 MS.gene20141:CDS
ACGTACATAGTAAGCAGTGA+CGG 0.682695 4.4:+3638624 None:intergenic
TCTTGGTGGGATCAAACCAG+AGG 0.721184 4.4:+3638343 None:intergenic
TGGTGCAGTTGTCATCACAA+GGG 0.724915 4.4:-3638479 MS.gene20141:intron
CGTGCTAAGATATTCAACGG+TGG 0.766280 4.4:-3638766 MS.gene20141:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTACTTACATAATATGTTG+GGG + chr4.4:3638408-3638427 None:intergenic 20.0%
!! GATATATAAATACAGATTCT+TGG - chr4.4:3638515-3638534 MS.gene20141:intron 20.0%
!! GATTACTTACATAATATGTT+GGG + chr4.4:3638409-3638428 None:intergenic 20.0%
!! TGATTACTTACATAATATGT+TGG + chr4.4:3638410-3638429 None:intergenic 20.0%
! AATTGATATGCAACTCAAAT+TGG - chr4.4:3638028-3638047 MS.gene20141:CDS 25.0%
! AGACTTGAATATATTCTCAT+TGG + chr4.4:3638609-3638628 None:intergenic 25.0%
! TTGAATATATTCTCATTGGT+TGG + chr4.4:3638605-3638624 None:intergenic 25.0%
!!! GAAATTGATTTTGAGTTCTT+GGG - chr4.4:3638252-3638271 MS.gene20141:intron 25.0%
!!! TGAAATTGATTTTGAGTTCT+TGG - chr4.4:3638251-3638270 MS.gene20141:intron 25.0%
AAATCAATTTCATCGTGAGT+TGG + chr4.4:3638243-3638262 None:intergenic 30.0%
ATGAGTTTGAATGAATGAGA+TGG + chr4.4:3638107-3638126 None:intergenic 30.0%
CATTCTTGAAGATCCTTATT+GGG + chr4.4:3638551-3638570 None:intergenic 30.0%
GAATGAATGAGATGGAAAAA+AGG + chr4.4:3638099-3638118 None:intergenic 30.0%
TGAGAATATATTCAAGTCTC+TGG - chr4.4:3638610-3638629 MS.gene20141:intron 30.0%
TTCAAGAATGCTGAATCTAT+TGG - chr4.4:3638561-3638580 MS.gene20141:intron 30.0%
! TTTTACGAGCATTTTCCTTA+TGG - chr4.4:3638197-3638216 MS.gene20141:intron 30.0%
!! ACCAAGATTTTGATCTAACA+TGG - chr4.4:3637898-3637917 MS.gene20141:CDS 30.0%
!! CAAGATTTTGATCTAACATG+GGG - chr4.4:3637900-3637919 MS.gene20141:CDS 30.0%
!! CCAAGATTTTGATCTAACAT+GGG - chr4.4:3637899-3637918 MS.gene20141:CDS 30.0%
!! TCCAAGAAAGAGTACTTATT+CGG - chr4.4:3638002-3638021 MS.gene20141:CDS 30.0%
!!! AGCTTTTTCATGACATCAAA+AGG - chr4.4:3638174-3638193 MS.gene20141:intron 30.0%
ACCGAATAAGTACTCTTTCT+TGG + chr4.4:3638006-3638025 None:intergenic 35.0%
ATAAGTACTCTTTCTTGGAC+TGG + chr4.4:3638001-3638020 None:intergenic 35.0%
ATGGTAGAAGAAGATTGAGA+TGG - chr4.4:3638793-3638812 MS.gene20141:CDS 35.0%
CCCATGTTAGATCAAAATCT+TGG + chr4.4:3637902-3637921 None:intergenic 35.0%
GCATTCTTGAAGATCCTTAT+TGG + chr4.4:3638552-3638571 None:intergenic 35.0%
TCCAAATGATAGAGTAAGTG+TGG + chr4.4:3638383-3638402 None:intergenic 35.0%
TCCACACTTACTCTATCATT+TGG - chr4.4:3638379-3638398 MS.gene20141:CDS 35.0%
TGGTAGAAGAAGATTGAGAT+GGG - chr4.4:3638794-3638813 MS.gene20141:CDS 35.0%
! ATTCTCATTGGTTGGTTCTT+TGG + chr4.4:3638597-3638616 None:intergenic 35.0%
! TTCTCATTGGTTGGTTCTTT+GGG + chr4.4:3638596-3638615 None:intergenic 35.0%
!! GTTCAGTAGCTTTGAAATTG+CGG + chr4.4:3638710-3638729 None:intergenic 35.0%
ACGTACATAGTAAGCAGTGA+CGG + chr4.4:3638075-3638094 None:intergenic 40.0%
ACGTTTGTGTGGAGAATGTA+AGG + chr4.4:3638294-3638313 None:intergenic 40.0%
AGAGTAAGTGTGGAAGTTCT+TGG + chr4.4:3638373-3638392 None:intergenic 40.0%
ATGCAACTCAAATTGGTTGC+TGG - chr4.4:3638035-3638054 MS.gene20141:CDS 40.0%
CACCGTGCTAAGATATTCAA+CGG - chr4.4:3637927-3637946 MS.gene20141:CDS 40.0%
CACCGTTGAATATCTTAGCA+CGG + chr4.4:3637932-3637951 None:intergenic 40.0%
CGCATGAACAATATGCTGAT+TGG - chr4.4:3637828-3637847 MS.gene20141:CDS 40.0%
CTTGAGAAAACACGTTTGTG+TGG + chr4.4:3638305-3638324 None:intergenic 40.0%
GAGAACAACAATTCTACCTC+TGG - chr4.4:3638337-3638356 MS.gene20141:CDS 40.0%
GATCTTAAGCGATTTCCACA+AGG - chr4.4:3638852-3638871 MS.gene20141:CDS 40.0%
TAAGTGTGGAAGTTCTTGGT+GGG + chr4.4:3638369-3638388 None:intergenic 40.0%
TGCAGAATCAGAGAATGAAG+TGG + chr4.4:3638748-3638767 None:intergenic 40.0%
TGGTTGACAACATCCCAATA+AGG - chr4.4:3638535-3638554 MS.gene20141:intron 40.0%
! AACGTGTTTTCTCAAGGCAA+AGG - chr4.4:3638309-3638328 MS.gene20141:CDS 40.0%
! ATTGGTTGGTTCTTTGGGAA+AGG + chr4.4:3638591-3638610 None:intergenic 40.0%
! CACACAAACGTGTTTTCTCA+AGG - chr4.4:3638303-3638322 MS.gene20141:CDS 40.0%
!! GTAAGCTCTAAATGGTGCTT+TGG + chr4.4:3638685-3638704 None:intergenic 40.0%
!! GTGGTTTGGTGAAAACTGAT+TGG - chr4.4:3638658-3638677 MS.gene20141:CDS 40.0%
!! TTGCGGTAGTAAGCTCTAAA+TGG + chr4.4:3638693-3638712 None:intergenic 40.0%
AGCAACGAGCTTGATGCTTA+TGG - chr4.4:3638774-3638793 MS.gene20141:CDS 45.0%
ATGGTGCAGTTGTCATCACA+AGG - chr4.4:3638216-3638235 MS.gene20141:intron 45.0%
CGTGCTAAGATATTCAACGG+TGG - chr4.4:3637930-3637949 MS.gene20141:CDS 45.0%
CTTTCTCTCGACAAAGTCTC+CGG - chr4.4:3637966-3637985 MS.gene20141:CDS 45.0%
GAGTTCTTGGGAAACGTTAG+CGG - chr4.4:3638264-3638283 MS.gene20141:intron 45.0%
GTAAGTGTGGAAGTTCTTGG+TGG + chr4.4:3638370-3638389 None:intergenic 45.0%
GTGATGACAACTGCACCATA+AGG + chr4.4:3638215-3638234 None:intergenic 45.0%
TGCAGAGCATGTAGCTATCA+AGG + chr4.4:3637871-3637890 None:intergenic 45.0%
TGGTGCAGTTGTCATCACAA+GGG - chr4.4:3638217-3638236 MS.gene20141:intron 45.0%
! AAGGTATTCCTGCTGAGTGT+AGG - chr4.4:3638871-3638890 MS.gene20141:CDS 45.0%
! AGTTTTCACCAAACCACCTC+TGG + chr4.4:3638655-3638674 None:intergenic 45.0%
ACCTCTGGTAGCCCAATCAT+CGG + chr4.4:3638640-3638659 None:intergenic 50.0%
CACTCAGCAGGAATACCTTG+TGG + chr4.4:3638870-3638889 None:intergenic 50.0%
TCTTGGTGGGATCAAACCAG+AGG + chr4.4:3638356-3638375 None:intergenic 50.0%
TTGGTTGCTGGAAACTCTGC+TGG - chr4.4:3638047-3638066 MS.gene20141:CDS 50.0%
!! TCTCTGGAACGCCGATGATT+GGG - chr4.4:3638626-3638645 MS.gene20141:intron 50.0%
CTGCTGAGTGTAGGCGTTCA+AGG - chr4.4:3638880-3638899 MS.gene20141:CDS 55.0%
CTTGAACGCCTACACTCAGC+AGG + chr4.4:3638882-3638901 None:intergenic 55.0%
GATGATTGGGCTACCAGAGG+TGG - chr4.4:3638639-3638658 MS.gene20141:CDS 55.0%
TTGGGCTACCAGAGGTGGTT+TGG - chr4.4:3638644-3638663 MS.gene20141:CDS 55.0%
!! GTCTCTGGAACGCCGATGAT+TGG - chr4.4:3638625-3638644 MS.gene20141:intron 55.0%
CTCGACAAAGTCTCCGGCTC+TGG - chr4.4:3637972-3637991 MS.gene20141:CDS 60.0%
GCCGATGATTGGGCTACCAG+AGG - chr4.4:3638636-3638655 MS.gene20141:CDS 60.0%
! CTTGGACTGGAAGCCAGAGC+CGG + chr4.4:3637988-3638007 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 3637810 3638908 3637810 ID=MS.gene20141
chr4.4 mRNA 3637810 3638908 3637810 ID=MS.gene20141.t1;Parent=MS.gene20141
chr4.4 exon 3638630 3638908 3638630 ID=MS.gene20141.t1.exon1;Parent=MS.gene20141.t1
chr4.4 CDS 3638630 3638908 3638630 ID=cds.MS.gene20141.t1;Parent=MS.gene20141.t1
chr4.4 exon 3638300 3638493 3638300 ID=MS.gene20141.t1.exon2;Parent=MS.gene20141.t1
chr4.4 CDS 3638300 3638493 3638300 ID=cds.MS.gene20141.t1;Parent=MS.gene20141.t1
chr4.4 exon 3637810 3638188 3637810 ID=MS.gene20141.t1.exon3;Parent=MS.gene20141.t1
chr4.4 CDS 3637810 3638188 3637810 ID=cds.MS.gene20141.t1;Parent=MS.gene20141.t1
Gene Sequence

>MS.gene20141

ATGGCGTCTTCTTGTATTCGCATGAACAATATGCTGATTGGAGCTATGATAGTTAGTTCCTTGATAGCTACATGCTCTGCAAATTTCTACCAAGATTTTGATCTAACATGGGGTGATCACCGTGCTAAGATATTCAACGGTGGTCAGCTTCTTTCACTTTCTCTCGACAAAGTCTCCGGCTCTGGCTTCCAGTCCAAGAAAGAGTACTTATTCGGTAGAATTGATATGCAACTCAAATTGGTTGCTGGAAACTCTGCTGGAACCGTCACTGCTTACTATGTACGTACCTTTTTTCCATCTCATTCATTCAAACTCATACACATTGATTCTTTCTTTTTCAAAAGCAACTTTAATCAACATACATAGCTTTTTCATGACATCAAAAGGTTTTACGAGCATTTTCCTTATGGTGCAGTTGTCATCACAAGGGCCAACTCACGATGAAATTGATTTTGAGTTCTTGGGAAACGTTAGCGGTGATCCTTACATTCTCCACACAAACGTGTTTTCTCAAGGCAAAGGAAACAGAGAACAACAATTCTACCTCTGGTTTGATCCCACCAAGAACTTCCACACTTACTCTATCATTTGGAAGCCCCAACATATTATGTAAGTAATCAAATTAAAACTAATATATTCATGTTCGAATAAACAACTTAGTTAAGTGCTTATAACACAAAACACTTCTAACTGAATTAACATGTTGATATATAAATACAGATTCTTGGTTGACAACATCCCAATAAGGATCTTCAAGAATGCTGAATCTATTGGTATTCCTTTCCCAAAGAACCAACCAATGAGAATATATTCAAGTCTCTGGAACGCCGATGATTGGGCTACCAGAGGTGGTTTGGTGAAAACTGATTGGTCCAAAGCACCATTTAGAGCTTACTACCGCAATTTCAAAGCTACTGAACTCTCATCTGTCTCTTCCACTTCATTCTCTGATTCTGCATTGCAGAGCAACGAGCTTGATGCTTATGGTAGAAGAAGATTGAGATGGGTTCAGAAGTATTTCATGATATATAATTACTGCAATGATCTTAAGCGATTTCCACAAGGTATTCCTGCTGAGTGTAGGCGTTCAAGGTTCTGA
Protein sequence

>MS.gene20141.t1

MASSCIRMNNMLIGAMIVSSLIATCSANFYQDFDLTWGDHRAKIFNGGQLLSLSLDKVSGSGFQSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGPTHDEIDFEFLGNVSGDPYILHTNVFSQGKGNREQQFYLWFDPTKNFHTYSIIWKPQHIIFLVDNIPIRIFKNAESIGIPFPKNQPMRIYSSLWNADDWATRGGLVKTDWSKAPFRAYYRNFKATELSSVSSTSFSDSALQSNELDAYGRRRLRWVQKYFMIYNYCNDLKRFPQGIPAECRRSRF