AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20158

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20158.t1 MTR_8g101890 97.541 122 3 0 1 122 1 122 2.03e-87 249
MS.gene20158.t1 MTR_8g101880 79.167 120 25 0 3 122 2 121 2.68e-71 209
MS.gene20158.t1 MTR_2g100380 63.115 122 45 0 1 122 96 217 2.15e-54 169
MS.gene20158.t1 MTR_5g062980 80.882 68 9 1 59 122 1 68 1.15e-33 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20158.t1 AT4G26210 82.500 120 21 0 1 120 1 120 1.18e-71 210
MS.gene20158.t1 AT4G26210 82.500 120 21 0 1 120 1 120 1.18e-71 210
MS.gene20158.t1 AT4G29480 80.000 120 24 0 1 120 1 120 5.66e-70 206
MS.gene20158.t1 AT2G19680 81.667 120 22 0 1 120 1 120 2.78e-69 204
MS.gene20158.t1 AT2G19680 81.667 120 22 0 1 120 1 120 2.78e-69 204

Find 28 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATGTTTCGACACAAAT+TGG 0.188882 4.4:+3821043 None:intergenic
CGGTTCGTTGTGAGGCCTTC+TGG 0.193200 4.4:-3819190 MS.gene20158:CDS
TTGATTATGTCAAGCATTTA+TGG 0.216096 4.4:-3819160 MS.gene20158:CDS
GAGGAACAGGCAAGACTTAA+AGG 0.283541 4.4:-3819138 MS.gene20158:CDS
GCAGGCATTGCTGCTTTGTT+TGG 0.306097 4.4:-3819107 MS.gene20158:CDS
CATTGCTGCTTTGTTTGGCT+TGG 0.320848 4.4:-3819102 MS.gene20158:CDS
TCAAGCATTTATGGAGGAAC+AGG 0.424738 4.4:-3819151 MS.gene20158:CDS
TCCATGTTTCGACACAAATT+GGG 0.433923 4.4:+3821044 None:intergenic
TGGTTACTACAAGCAATTGT+TGG 0.474911 4.4:-3821019 MS.gene20158:CDS
GGAAGGGGATTTACTTTCAC+CGG 0.482572 4.4:-3819044 MS.gene20158:CDS
TATTGTGCTGGTGAGATTGT+TGG 0.487898 4.4:-3819065 MS.gene20158:CDS
GACTTAAAGGTTGAAGATGC+AGG 0.493818 4.4:-3819125 MS.gene20158:CDS
CAGCATTCCGGTTCGTTGTG+AGG 0.494395 4.4:-3819198 MS.gene20158:CDS
TTTGTGTCGAAACATGGAAC+TGG 0.497463 4.4:-3821039 MS.gene20158:CDS
CAGAAGGCCTCACAACGAAC+CGG 0.500486 4.4:+3819191 None:intergenic
TTGCATTTCTCAATAGTAGC+AGG 0.501942 4.4:+3820970 None:intergenic
TTCAATGGCGTCGAAGTTGA+AGG 0.508306 4.4:-3821094 None:intergenic
TACTTTACACACGCCTTGCT+AGG 0.522831 4.4:-3820930 MS.gene20158:intron
GCTGGTGAGATTGTTGGAAG+GGG 0.524753 4.4:-3819059 MS.gene20158:CDS
TGCTATGCATGGTATTGTGC+TGG 0.532565 4.4:-3819077 MS.gene20158:CDS
TGCTGGTGAGATTGTTGGAA+GGG 0.539629 4.4:-3819060 MS.gene20158:CDS
ATAAATTATATAACCTAGCA+AGG 0.541358 4.4:+3820917 None:intergenic
TCCCAATTTGTGTCGAAACA+TGG 0.545137 4.4:-3821045 MS.gene20158:CDS
TCGTTGTGAGGCCTTCTGGA+AGG 0.586771 4.4:-3819186 MS.gene20158:CDS
TTGGCTTGGAATGCTATGCA+TGG 0.606806 4.4:-3819088 MS.gene20158:CDS
ATAATCAAGTTCCTTCCAGA+AGG 0.620068 4.4:+3819175 None:intergenic
ATTATGTCAAGCATTTATGG+AGG 0.623689 4.4:-3819157 MS.gene20158:CDS
GTGCTGGTGAGATTGTTGGA+AGG 0.633241 4.4:-3819061 MS.gene20158:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAATATATTATGTC+AGG + chr4.4:3820171-3820190 None:intergenic 10.0%
!! GAAAAAAAAAAGGATAAAAT+AGG - chr4.4:3819407-3819426 MS.gene20158:intron 15.0%
!! AAAAAAAAGGATAAAATAGG+TGG - chr4.4:3819410-3819429 MS.gene20158:intron 20.0%
!! AGTGTAAATAAAATAAAGGA+AGG - chr4.4:3819725-3819744 MS.gene20158:intron 20.0%
!! ATAAATTATATAACCTAGCA+AGG + chr4.4:3819207-3819226 None:intergenic 20.0%
!! CAATATATTAAGAAGAAAGT+TGG + chr4.4:3819233-3819252 None:intergenic 20.0%
!! TAATCATTAGAAAGAAAGTT+GGG - chr4.4:3819616-3819635 MS.gene20158:intron 20.0%
!! TATAAACAATCTATGGATAT+CGG + chr4.4:3820593-3820612 None:intergenic 20.0%
!! TGTTTAATGCGAAAAAAAAA+AGG - chr4.4:3819397-3819416 MS.gene20158:intron 20.0%
!! TTAATCATTAGAAAGAAAGT+TGG - chr4.4:3819615-3819634 MS.gene20158:intron 20.0%
!! TTATGAGTATAAACAATCTA+TGG + chr4.4:3820600-3820619 None:intergenic 20.0%
!!! AACTAGTTTTAAAATGATGT+TGG + chr4.4:3820716-3820735 None:intergenic 20.0%
!!! AAGGAGTGTAAATAAAATAA+AGG - chr4.4:3819721-3819740 MS.gene20158:intron 20.0%
!!! ACATTTTTTATCTTGACATA+TGG - chr4.4:3820426-3820445 MS.gene20158:intron 20.0%
!!! ATTTTAAAGAAAATGAATGG+TGG - chr4.4:3820390-3820409 MS.gene20158:intron 20.0%
!!! CCAATTTTAAAGAAAATGAA+TGG - chr4.4:3820387-3820406 MS.gene20158:intron 20.0%
!!! CCATTCATTTTCTTTAAAAT+TGG + chr4.4:3820390-3820409 None:intergenic 20.0%
! AAAATTGTCTTACTCTTCAT+TGG + chr4.4:3819480-3819499 None:intergenic 25.0%
! AAGGATAATTCTATAGTCAA+AGG - chr4.4:3819744-3819763 MS.gene20158:intron 25.0%
! AATCAACATATTATAGACCA+AGG + chr4.4:3819284-3819303 None:intergenic 25.0%
! ATCAAACTTTATGAAAGACT+TGG - chr4.4:3820057-3820076 MS.gene20158:intron 25.0%
! ATCAACATATTATAGACCAA+GGG + chr4.4:3819283-3819302 None:intergenic 25.0%
! CAAACATGAAAAAACTAAAG+TGG + chr4.4:3820455-3820474 None:intergenic 25.0%
! CTTCATAATCTATCATTGTA+TGG - chr4.4:3820485-3820504 MS.gene20158:intron 25.0%
! TAACTCTAAATCTCTCATAA+TGG + chr4.4:3819802-3819821 None:intergenic 25.0%
! TATATTATGTCAGGTTACTT+TGG + chr4.4:3820162-3820181 None:intergenic 25.0%
! TGAATTAGCTAAACAATCAT+GGG - chr4.4:3820779-3820798 MS.gene20158:intron 25.0%
! TTGATTATGTCAAGCATTTA+TGG - chr4.4:3820961-3820980 MS.gene20158:CDS 25.0%
! TTTAGCTAATTCACTAAAGA+AGG + chr4.4:3820772-3820791 None:intergenic 25.0%
!! AATTAAATTTTGCAGCATTC+CGG - chr4.4:3820911-3820930 MS.gene20158:intron 25.0%
!! TTTAAAATGATGTTGGAATG+TGG + chr4.4:3820709-3820728 None:intergenic 25.0%
!! TTTACCAAAGTCACATTTTT+AGG - chr4.4:3820189-3820208 MS.gene20158:intron 25.0%
!!! CAAGTTGACTATTTATTTTG+TGG + chr4.4:3820362-3820381 None:intergenic 25.0%
!!! TCACATTTTTAGGAGTAAAT+GGG - chr4.4:3820199-3820218 MS.gene20158:intron 25.0%
AAAAGGACTTTAAGTGGTAT+GGG - chr4.4:3820306-3820325 MS.gene20158:intron 30.0%
ACCACAGAAAACAGAAAAAA+TGG + chr4.4:3820275-3820294 None:intergenic 30.0%
ATTATGTCAAGCATTTATGG+AGG - chr4.4:3820964-3820983 MS.gene20158:CDS 30.0%
CATCACTTATAGTTAAACCA+AGG - chr4.4:3820115-3820134 MS.gene20158:intron 30.0%
CTTATAGTTAAACCAAGGTT+TGG - chr4.4:3820120-3820139 MS.gene20158:intron 30.0%
GAATTAGCTAAACAATCATG+GGG - chr4.4:3820780-3820799 MS.gene20158:intron 30.0%
GGAATTAATCAAGACAAGAT+AGG - chr4.4:3819585-3819604 MS.gene20158:intron 30.0%
GGTAAAACAAAAAATGCCTT+GGG - chr4.4:3820244-3820263 MS.gene20158:intron 30.0%
GGTTGGATTTAATTCATACA+GGG - chr4.4:3819831-3819850 MS.gene20158:intron 30.0%
GTGAATTAGCTAAACAATCA+TGG - chr4.4:3820778-3820797 MS.gene20158:intron 30.0%
TAAAGCAAAAGGACTTTAAG+TGG - chr4.4:3820300-3820319 MS.gene20158:intron 30.0%
TAATCAAGACAAGATAGGAT+AGG - chr4.4:3819590-3819609 MS.gene20158:intron 30.0%
TACTCCTAAAAATGTGACTT+TGG + chr4.4:3820196-3820215 None:intergenic 30.0%
TCTATAGTCAAAGGTAGAAT+GGG - chr4.4:3819753-3819772 MS.gene20158:intron 30.0%
TGGTTGGATTTAATTCATAC+AGG - chr4.4:3819830-3819849 MS.gene20158:intron 30.0%
TTATGAGCTGATCATATTCA+AGG + chr4.4:3820523-3820542 None:intergenic 30.0%
TTCAAACATCAAACAGAACT+GGG + chr4.4:3820667-3820686 None:intergenic 30.0%
TTCTATAGTCAAAGGTAGAA+TGG - chr4.4:3819752-3819771 MS.gene20158:intron 30.0%
! TATGAGAGATTTAGAGTTAC+TGG - chr4.4:3819803-3819822 MS.gene20158:intron 30.0%
!! AAATGAATGGTGGTTGTTTT+TGG - chr4.4:3820400-3820419 MS.gene20158:intron 30.0%
!! AGAAGCACTGAAAACTAAAT+TGG + chr4.4:3820867-3820886 None:intergenic 30.0%
!! GTCACATTTTTAGGAGTAAA+TGG - chr4.4:3820198-3820217 MS.gene20158:intron 30.0%
AAAGGACTTTAAGTGGTATG+GGG - chr4.4:3820307-3820326 MS.gene20158:intron 35.0%
AGTGTTGCAACAATGAGAAT+AGG - chr4.4:3819558-3819577 MS.gene20158:intron 35.0%
ATAATCAAGTTCCTTCCAGA+AGG + chr4.4:3820949-3820968 None:intergenic 35.0%
CAAAAGGACTTTAAGTGGTA+TGG - chr4.4:3820305-3820324 MS.gene20158:intron 35.0%
GAACTACAGTAGCATTACTA+AGG - chr4.4:3819702-3819721 MS.gene20158:intron 35.0%
GCAACAATGAGAATAGGAAT+AGG - chr4.4:3819564-3819583 MS.gene20158:intron 35.0%
GGGTAAAACAAAAAATGCCT+TGG - chr4.4:3820243-3820262 MS.gene20158:intron 35.0%
GTTCAAACATCAAACAGAAC+TGG + chr4.4:3820668-3820687 None:intergenic 35.0%
TAAACAATCATGGGGAAATG+TGG - chr4.4:3820788-3820807 MS.gene20158:intron 35.0%
TAGTTGTCAGGGAAAAACTT+TGG - chr4.4:3819892-3819911 MS.gene20158:intron 35.0%
TATCACTCCATCATTGTATC+TGG + chr4.4:3819440-3819459 None:intergenic 35.0%
TCATTAGAAAGAAAGTTGGG+TGG - chr4.4:3819619-3819638 MS.gene20158:intron 35.0%
TCCATGTTTCGACACAAATT+GGG + chr4.4:3819080-3819099 None:intergenic 35.0%
TGGTTACTACAAGCAATTGT+TGG - chr4.4:3819102-3819121 MS.gene20158:CDS 35.0%
TGTGGTTAGTGTAAAGCAAA+AGG - chr4.4:3820289-3820308 MS.gene20158:intron 35.0%
TTCCATGTTTCGACACAAAT+TGG + chr4.4:3819081-3819100 None:intergenic 35.0%
TTGCATTTCTCAATAGTAGC+AGG + chr4.4:3819154-3819173 None:intergenic 35.0%
! ATACAATGATGGAGTGATAG+AGG - chr4.4:3819441-3819460 MS.gene20158:intron 35.0%
! ATGGGATCAAGGAAAAGTAT+AGG - chr4.4:3819771-3819790 MS.gene20158:intron 35.0%
! ATTTTACCTCCTTCAAATGG+GGG - chr4.4:3820222-3820241 MS.gene20158:intron 35.0%
! GATTTTACCTCCTTCAAATG+GGG - chr4.4:3820221-3820240 MS.gene20158:intron 35.0%
! GGATTTTACCTCCTTCAAAT+GGG - chr4.4:3820220-3820239 MS.gene20158:intron 35.0%
! TGATTGGTAATACGATCAGT+CGG - chr4.4:3819333-3819352 MS.gene20158:intron 35.0%
! TTGATTTTGCTTCCAAACCT+TGG + chr4.4:3820135-3820154 None:intergenic 35.0%
! TTTTTCCCTGACAACTAGAT+GGG + chr4.4:3819889-3819908 None:intergenic 35.0%
!!! GCCATTTTTTCTGTTTTCTG+TGG - chr4.4:3820271-3820290 MS.gene20158:intron 35.0%
ACATCTGTCAAGTCTGTTCT+AGG - chr4.4:3820841-3820860 MS.gene20158:intron 40.0%
ACTAGATGGGGTAAACAACA+TGG + chr4.4:3819876-3819895 None:intergenic 40.0%
AGAAAAAATGGCACTTCCCA+AGG + chr4.4:3820263-3820282 None:intergenic 40.0%
AGCTACGTCTTAGGTGATTT+GGG - chr4.4:3819666-3819685 MS.gene20158:intron 40.0%
GACTTAAAGGTTGAAGATGC+AGG - chr4.4:3820996-3821015 MS.gene20158:CDS 40.0%
GATGTGTGTGACATTTGATC+AGG + chr4.4:3820826-3820845 None:intergenic 40.0%
GGCAGTTTGGCAGTTTAATT+CGG - chr4.4:3819354-3819373 MS.gene20158:intron 40.0%
GTGATGTTAGACAACCAGAT+CGG - chr4.4:3819308-3819327 MS.gene20158:intron 40.0%
TATTACCAATCAGTCCGATC+TGG + chr4.4:3819325-3819344 None:intergenic 40.0%
TCAAAGGTAGAATGGGATCA+AGG - chr4.4:3819760-3819779 MS.gene20158:intron 40.0%
TCAAGCATTTATGGAGGAAC+AGG - chr4.4:3820970-3820989 MS.gene20158:CDS 40.0%
TCCCAATTTGTGTCGAAACA+TGG - chr4.4:3819076-3819095 MS.gene20158:CDS 40.0%
TCTATGTTCATGTATGCCCT+TGG - chr4.4:3819264-3819283 MS.gene20158:intron 40.0%
TTCTCAACCTGTGCATGTTT+GGG - chr4.4:3819959-3819978 MS.gene20158:intron 40.0%
TTTAATTCATACAGGGCACG+AGG - chr4.4:3819838-3819857 MS.gene20158:intron 40.0%
TTTACCCCATCTAGTTGTCA+GGG - chr4.4:3819881-3819900 MS.gene20158:intron 40.0%
TTTCTCAACCTGTGCATGTT+TGG - chr4.4:3819958-3819977 MS.gene20158:intron 40.0%
TTTGTGTCGAAACATGGAAC+TGG - chr4.4:3819082-3819101 MS.gene20158:CDS 40.0%
! AGACAATTTTACCGTGCATC+TGG - chr4.4:3819490-3819509 MS.gene20158:intron 40.0%
! GACAATTTTACCGTGCATCT+GGG - chr4.4:3819491-3819510 MS.gene20158:intron 40.0%
! GATTTAGAGTTACTGGTCTG+TGG - chr4.4:3819810-3819829 MS.gene20158:intron 40.0%
! GGGATTTTACCTCCTTCAAA+TGG - chr4.4:3820219-3820238 MS.gene20158:intron 40.0%
! GTTTTTCCCTGACAACTAGA+TGG + chr4.4:3819890-3819909 None:intergenic 40.0%
! TATTGTGCTGGTGAGATTGT+TGG - chr4.4:3821056-3821075 MS.gene20158:CDS 40.0%
! TTAGGTGATTTGGGCATGTA+TGG - chr4.4:3819675-3819694 MS.gene20158:intron 40.0%
! TTTTACCTCCTTCAAATGGG+GGG - chr4.4:3820223-3820242 MS.gene20158:intron 40.0%
! TTTTCCCTGACAACTAGATG+GGG + chr4.4:3819888-3819907 None:intergenic 40.0%
!! TTGTTTTACCCCCCATTTGA+AGG + chr4.4:3820234-3820253 None:intergenic 40.0%
AATACGATCAGTCGGCAGTT+TGG - chr4.4:3819341-3819360 MS.gene20158:intron 45.0%
CACTCCATCATTGTATCTGG+TGG + chr4.4:3819437-3819456 None:intergenic 45.0%
CTCTGCCATCCTTTCTATGA+GGG + chr4.4:3819648-3819667 None:intergenic 45.0%
GAGCTACGTCTTAGGTGATT+TGG - chr4.4:3819665-3819684 MS.gene20158:intron 45.0%
GAGGAACAGGCAAGACTTAA+AGG - chr4.4:3820983-3821002 MS.gene20158:CDS 45.0%
GGAAGGGGATTTACTTTCAC+CGG - chr4.4:3821077-3821096 MS.gene20158:CDS 45.0%
GGTTTCCCTCATAGAAAGGA+TGG - chr4.4:3819640-3819659 MS.gene20158:intron 45.0%
GTTTACCCCATCTAGTTGTC+AGG - chr4.4:3819880-3819899 MS.gene20158:intron 45.0%
TACTTTACACACGCCTTGCT+AGG - chr4.4:3819191-3819210 MS.gene20158:CDS 45.0%
TCATTCACCCAAACATGCAC+AGG + chr4.4:3819969-3819988 None:intergenic 45.0%
TCTTCATTGGATCCTACGCT+AGG + chr4.4:3819467-3819486 None:intergenic 45.0%
TGCTATGCATGGTATTGTGC+TGG - chr4.4:3821044-3821063 MS.gene20158:CDS 45.0%
TGCTGGTGAGATTGTTGGAA+GGG - chr4.4:3821061-3821080 MS.gene20158:CDS 45.0%
TGGTCCACCAGATACAATGA+TGG - chr4.4:3819430-3819449 MS.gene20158:intron 45.0%
! TAGAGTTACTGGTCTGTGGT+TGG - chr4.4:3819814-3819833 MS.gene20158:intron 45.0%
! TGCACAGGTTGAGAAAGCAA+AGG + chr4.4:3819954-3819973 None:intergenic 45.0%
! TTTTACCCCCCATTTGAAGG+AGG + chr4.4:3820231-3820250 None:intergenic 45.0%
!! CATTGCTGCTTTGTTTGGCT+TGG - chr4.4:3821019-3821038 MS.gene20158:CDS 45.0%
!! TTGGCTTGGAATGCTATGCA+TGG - chr4.4:3821033-3821052 MS.gene20158:CDS 45.0%
GACAACCAGATCGGACTGAT+TGG - chr4.4:3819317-3819336 MS.gene20158:intron 50.0%
GCTCTGCCATCCTTTCTATG+AGG + chr4.4:3819649-3819668 None:intergenic 50.0%
GCTGGTGAGATTGTTGGAAG+GGG - chr4.4:3821062-3821081 MS.gene20158:CDS 50.0%
GGAGTCCTGCAATGTTGTGT+GGG - chr4.4:3819512-3819531 MS.gene20158:intron 50.0%
GGGTGGTTTCCCTCATAGAA+AGG - chr4.4:3819636-3819655 MS.gene20158:intron 50.0%
GTTGTGTGGGACGAAATGCT+GGG - chr4.4:3819525-3819544 MS.gene20158:intron 50.0%
TGTTGTGTGGGACGAAATGC+TGG - chr4.4:3819524-3819543 MS.gene20158:intron 50.0%
TTCGTCCCACACAACATTGC+AGG + chr4.4:3819520-3819539 None:intergenic 50.0%
! GTGCTGGTGAGATTGTTGGA+AGG - chr4.4:3821060-3821079 MS.gene20158:CDS 50.0%
!! GCAGGCATTGCTGCTTTGTT+TGG - chr4.4:3821014-3821033 MS.gene20158:CDS 50.0%
CAGAAGGCCTCACAACGAAC+CGG + chr4.4:3820933-3820952 None:intergenic 55.0%
GAGTGATAGAGGCCTAGCGT+AGG - chr4.4:3819452-3819471 MS.gene20158:intron 55.0%
GGATGGCAGAGCTACGTCTT+AGG - chr4.4:3819657-3819676 MS.gene20158:intron 55.0%
GGGAGTCCTGCAATGTTGTG+TGG - chr4.4:3819511-3819530 MS.gene20158:intron 55.0%
TCGTTGTGAGGCCTTCTGGA+AGG - chr4.4:3820935-3820954 MS.gene20158:CDS 55.0%
TTGCAGGACTCCCAGATGCA+CGG + chr4.4:3819504-3819523 None:intergenic 55.0%
! CAGCATTCCGGTTCGTTGTG+AGG - chr4.4:3820923-3820942 MS.gene20158:intron 55.0%
CGGTTCGTTGTGAGGCCTTC+TGG - chr4.4:3820931-3820950 MS.gene20158:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 3819031 3821112 3819031 ID=MS.gene20158
chr4.4 mRNA 3819031 3821112 3819031 ID=MS.gene20158.t1;Parent=MS.gene20158
chr4.4 exon 3820931 3821112 3820931 ID=MS.gene20158.t1.exon1;Parent=MS.gene20158.t1
chr4.4 CDS 3820931 3821112 3820931 ID=cds.MS.gene20158.t1;Parent=MS.gene20158.t1
chr4.4 exon 3819031 3819217 3819031 ID=MS.gene20158.t1.exon2;Parent=MS.gene20158.t1
chr4.4 CDS 3819031 3819217 3819031 ID=cds.MS.gene20158.t1;Parent=MS.gene20158.t1
Gene Sequence

>MS.gene20158

ATGGCGTCGAAGTTGAAGGCATTGCAGTCAAAAGCATGTCAAGCATCCCAATTTGTGTCGAAACATGGAACTGGTTACTACAAGCAATTGTTGGAGCAGAACAAGCAATACATTCAAGAGCCTGCTACTATTGAGAAATGCAATCTTCTCGCTAAGCAATTACTTTACACACGCCTTGCTAGGTTATATAATTTATTCTCCAACTTTCTTCTTAATATATTGTGTGCTAAAATTCTATGTTCATGTATGCCCTTGGTCTATAATATGTTGATTTCTAGTGATGTTAGACAACCAGATCGGACTGATTGGTAATACGATCAGTCGGCAGTTTGGCAGTTTAATTCGGAACTGATAAGAAGTACAATATGTTTAATGCGAAAAAAAAAAGGATAAAATAGGTGGTCCACCAGATACAATGATGGAGTGATAGAGGCCTAGCGTAGGATCCAATGAAGAGTAAGACAATTTTACCGTGCATCTGGGAGTCCTGCAATGTTGTGTGGGACGAAATGCTGGGCAGTAAGTTCAGTGTTGCAACAATGAGAATAGGAATAGGAATTAATCAAGACAAGATAGGATAGGAATTAATCATTAGAAAGAAAGTTGGGTGGTTTCCCTCATAGAAAGGATGGCAGAGCTACGTCTTAGGTGATTTGGGCATGTATGGTGAAGAACTACAGTAGCATTACTAAGGAGTGTAAATAAAATAAAGGAAGGATAATTCTATAGTCAAAGGTAGAATGGGATCAAGGAAAAGTATAGGTAAGACCATTATGAGAGATTTAGAGTTACTGGTCTGTGGTTGGATTTAATTCATACAGGGCACGAGGACATTGTTTGAGCCATGTTGTTTACCCCATCTAGTTGTCAGGGAAAAACTTTGGTTGCTGTATTGTTAACTTCTAATATAAGTCTAGCATCCTTTGCTTTCTCAACCTGTGCATGTTTGGGTGAATGAATTTGAAAGAAACTGATTATGTTAAACTGAGCTTGAATTGAAGTGATTTATGTTTGATTGTTTTCTAAATCAAACTTTATGAAAGACTTGGTGTAAATTTTTCAACACAATGCAAAAGCTACTTTTCATCACTTATAGTTAAACCAAGGTTTGGAAGCAAAATCAATGATCCAAAGTAACCTGACATAATATATTTTTTTTTTACCAAAGTCACATTTTTAGGAGTAAATGGGATTTTACCTCCTTCAAATGGGGGGTAAAACAAAAAATGCCTTGGGAAGTGCCATTTTTTCTGTTTTCTGTGGTTAGTGTAAAGCAAAAGGACTTTAAGTGGTATGGGGTGTAGAAATGCATGTGATTTTCTTGATCACCACAAAATAAATAGTCAACTTGACTTGCCAATTTTAAAGAAAATGAATGGTGGTTGTTTTTGGAAAACATTTTTTATCTTGACATATGGCAACCACTTTAGTTTTTTCATGTTTGAAGTTTTTTTCTTCATAATCTATCATTGTATGGTTTGATTATATTCCTTGAATATGATCAGCTCATAATGTGCTTGTGAAGATTTACAATAATGTGAATAATCATGACAATAATGCCGATATCCATAGATTGTTTATACTCATAATATTGTTGTCAAACAACTTGCTGCTTGTAGTTCTTGTGATGAAGCCCAGTTCTGTTTGATGTTTGAACTTGAATTTTGCTTATGCGCCACATTCCAACATCATTTTAAAACTAGTTATGCGTAGCAAATTGTGCAACATATCTTCACAACCTTCTTTAGTGAATTAGCTAAACAATCATGGGGAAATGTGGAAACTTATTCTGCCTGATCAAATGTCACACACATCTGTCAAGTCTGTTCTAGGCCAATTTAGTTTTCAGTGCTTCTGCTTCTCTATATATAACTCACTCTAATTAAATTTTGCAGCATTCCGGTTCGTTGTGAGGCCTTCTGGAAGGAACTTGATTATGTCAAGCATTTATGGAGGAACAGGCAAGACTTAAAGGTTGAAGATGCAGGCATTGCTGCTTTGTTTGGCTTGGAATGCTATGCATGGTATTGTGCTGGTGAGATTGTTGGAAGGGGATTTACTTTCACCGGTTACTCTGTCTGA
Protein sequence

>MS.gene20158.t1

MASKLKALQSKACQASQFVSKHGTGYYKQLLEQNKQYIQEPATIEKCNLLAKQLLYTRLASIPVRCEAFWKELDYVKHLWRNRQDLKVEDAGIAALFGLECYAWYCAGEIVGRGFTFTGYSV