Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20159.t1 | XP_030488294.1 | 84.2 | 120 | 19 | 0 | 2 | 121 | 3 | 122 | 8.20E-54 | 219.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20159.t1 | A0A1S2YE81 | 84.2 | 120 | 19 | 0 | 2 | 121 | 3 | 122 | 7.7e-54 | 219.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050320 | MS.gene20159 | 0.803527 | 3.34E-49 | -1.69E-46 |
| MS.gene051756 | MS.gene20159 | 0.837656 | 4.57E-57 | -1.69E-46 |
| MS.gene051903 | MS.gene20159 | 0.818646 | 1.76E-52 | -1.69E-46 |
| MS.gene057094 | MS.gene20159 | 0.804385 | 2.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20159.t1 | MTR_8g101880 | 98.347 | 121 | 2 | 0 | 1 | 121 | 1 | 121 | 2.34e-87 | 249 |
| MS.gene20159.t1 | MTR_8g101890 | 78.333 | 120 | 26 | 0 | 2 | 121 | 3 | 122 | 1.83e-70 | 207 |
| MS.gene20159.t1 | MTR_2g100380 | 61.667 | 120 | 46 | 0 | 2 | 121 | 98 | 217 | 8.90e-52 | 162 |
| MS.gene20159.t1 | MTR_5g062980 | 69.492 | 59 | 18 | 0 | 58 | 116 | 1 | 59 | 2.33e-26 | 95.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20159.t1 | AT4G26210 | 73.950 | 119 | 31 | 0 | 2 | 120 | 3 | 121 | 5.23e-66 | 196 |
| MS.gene20159.t1 | AT4G26210 | 73.950 | 119 | 31 | 0 | 2 | 120 | 3 | 121 | 5.23e-66 | 196 |
| MS.gene20159.t1 | AT4G29480 | 73.950 | 119 | 31 | 0 | 2 | 120 | 3 | 121 | 9.15e-66 | 195 |
| MS.gene20159.t1 | AT2G19680 | 73.950 | 119 | 31 | 0 | 2 | 120 | 3 | 121 | 2.46e-65 | 194 |
| MS.gene20159.t1 | AT2G19680 | 73.950 | 119 | 31 | 0 | 2 | 120 | 3 | 121 | 2.46e-65 | 194 |
Find 23 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCATGCTTAGTTATAAAT+TGG | 0.149886 | 4.4:+3825710 | None:intergenic |
| TTGATTATGTCAAGCAAATA+TGG | 0.268577 | 4.4:-3823971 | MS.gene20159:CDS |
| TGAAAGGACTCACAACGATT+TGG | 0.345795 | 4.4:+3824002 | None:intergenic |
| AAAACCATGCATAGCATTCC+AGG | 0.362552 | 4.4:+3823895 | None:intergenic |
| TCCATGCTTAGTTATAAATT+GGG | 0.411328 | 4.4:+3825711 | None:intergenic |
| GAAGAACAAGGAATACATTC+AGG | 0.438061 | 4.4:-3825662 | MS.gene20159:CDS |
| GGAAGGGGATTTACTTTCAC+AGG | 0.448226 | 4.4:-3823855 | MS.gene20159:CDS |
| TGGCATTTCTCTACTGTAGC+AGG | 0.457610 | 4.4:+3825637 | None:intergenic |
| TTCTTACCACAAGCAGCTGT+TGG | 0.459464 | 4.4:-3825686 | MS.gene20159:CDS |
| TCGTTGTGAGTCCTTTCATA+AGG | 0.471099 | 4.4:-3823997 | MS.gene20159:CDS |
| TGCTGGTCAGATTGTTGGAA+GGG | 0.499361 | 4.4:-3823871 | MS.gene20159:CDS |
| AGTAGAGAAATGCCAACTTC+TGG | 0.523939 | 4.4:-3825629 | MS.gene20159:CDS |
| ATAGTTGCTTGGCCAGAAGT+TGG | 0.524773 | 4.4:+3825617 | None:intergenic |
| TCCCAATTTATAACTAAGCA+TGG | 0.525968 | 4.4:-3825712 | MS.gene20159:CDS |
| ATAATCAAGTTCCTTATGAA+AGG | 0.546893 | 4.4:+3823986 | None:intergenic |
| GCTGGTCAGATTGTTGGAAG+GGG | 0.563270 | 4.4:-3823870 | MS.gene20159:CDS |
| GGTTAAAATAAAACCTAGCA+AGG | 0.573767 | 4.4:+3825584 | None:intergenic |
| GTGCTGGTCAGATTGTTGGA+AGG | 0.577540 | 4.4:-3823872 | MS.gene20159:CDS |
| GCGAGTGTAAAATAGTTGCT+TGG | 0.582756 | 4.4:+3825606 | None:intergenic |
| TCTTCTCCAACAGCTGCTTG+TGG | 0.584533 | 4.4:+3825680 | None:intergenic |
| TTGGCCTGGAATGCTATGCA+TGG | 0.593962 | 4.4:-3823899 | MS.gene20159:CDS |
| GATTTGAAGATTGAAGATGC+AGG | 0.608817 | 4.4:-3823936 | MS.gene20159:CDS |
| GCAGCTGTTGGAGAAGAACA+AGG | 0.648148 | 4.4:-3825674 | MS.gene20159:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTTAAAAAAAAAAAATTG+AGG | + | chr4.4:3824553-3824572 | None:intergenic | 10.0% |
| !! | TCTTTAATTATTAAATATGA+TGG | - | chr4.4:3824723-3824742 | MS.gene20159:intron | 10.0% |
| !! | TAGATTTATTAATAAGGAAA+GGG | + | chr4.4:3824036-3824055 | None:intergenic | 15.0% |
| !!! | GTTTTATTAAAGAGTTAAAA+GGG | - | chr4.4:3824573-3824592 | MS.gene20159:intron | 15.0% |
| !!! | TGTTTTATTAAAGAGTTAAA+AGG | - | chr4.4:3824572-3824591 | MS.gene20159:intron | 15.0% |
| !! | AAAAATCACTTACAATCAAT+AGG | + | chr4.4:3824103-3824122 | None:intergenic | 20.0% |
| !! | AATAGAAACAAACTAAAAGT+AGG | + | chr4.4:3824658-3824677 | None:intergenic | 20.0% |
| !! | CTAGATTTATTAATAAGGAA+AGG | + | chr4.4:3824037-3824056 | None:intergenic | 20.0% |
| !! | TAGAAAAATACACGAAATTA+TGG | + | chr4.4:3824752-3824771 | None:intergenic | 20.0% |
| !! | TATACAGTATAAAAGATGAT+CGG | - | chr4.4:3824910-3824929 | MS.gene20159:intron | 20.0% |
| !!! | TTTTTTTTGCATTTTGATGT+CGG | - | chr4.4:3824129-3824148 | MS.gene20159:intron | 20.0% |
| ! | ATAATCAAGTTCCTTATGAA+AGG | + | chr4.4:3825613-3825632 | None:intergenic | 25.0% |
| ! | ATACAGTATAAAAGATGATC+GGG | - | chr4.4:3824911-3824930 | MS.gene20159:intron | 25.0% |
| ! | ATAGCTAAAAGAAGAAACTA+CGG | + | chr4.4:3825489-3825508 | None:intergenic | 25.0% |
| ! | GATTGAACAATCAACATATT+AGG | + | chr4.4:3824234-3824253 | None:intergenic | 25.0% |
| ! | TCCAGCTAGATTTATTAATA+AGG | + | chr4.4:3824042-3824061 | None:intergenic | 25.0% |
| ! | TCCATGCTTAGTTATAAATT+GGG | + | chr4.4:3823888-3823907 | None:intergenic | 25.0% |
| ! | TCCTTATTAATAAATCTAGC+TGG | - | chr4.4:3824038-3824057 | MS.gene20159:intron | 25.0% |
| ! | TTCCATGCTTAGTTATAAAT+TGG | + | chr4.4:3823889-3823908 | None:intergenic | 25.0% |
| ! | TTGATTATGTCAAGCAAATA+TGG | - | chr4.4:3825625-3825644 | MS.gene20159:CDS | 25.0% |
| ! | TTGTACTAAATAGCTTATTG+TGG | + | chr4.4:3825068-3825087 | None:intergenic | 25.0% |
| !! | ATATACCACTATAGCATTTT+AGG | - | chr4.4:3824837-3824856 | MS.gene20159:intron | 25.0% |
| !! | TATACCACTATAGCATTTTA+GGG | - | chr4.4:3824838-3824857 | MS.gene20159:intron | 25.0% |
| !!! | GTTTTGTTGTTTTGTACTTT+AGG | - | chr4.4:3824699-3824718 | MS.gene20159:intron | 25.0% |
| ACCATTAACTTTGTAGTTCA+GGG | + | chr4.4:3824275-3824294 | None:intergenic | 30.0% | |
| GGTTAAAATAAAACCTAGCA+AGG | + | chr4.4:3824015-3824034 | None:intergenic | 30.0% | |
| GTGAACTCTAAACTAACTAA+GGG | + | chr4.4:3825001-3825020 | None:intergenic | 30.0% | |
| TAAGACTTCATTGCTAAGAA+AGG | + | chr4.4:3824959-3824978 | None:intergenic | 30.0% | |
| TACAAAATACTGTGCAATAG+TGG | - | chr4.4:3825199-3825218 | MS.gene20159:intron | 30.0% | |
| TATAGTGGTATATCGAATAG+CGG | + | chr4.4:3824830-3824849 | None:intergenic | 30.0% | |
| TCCCAATTTATAACTAAGCA+TGG | - | chr4.4:3823884-3823903 | MS.gene20159:CDS | 30.0% | |
| TCGCTAATATATGTACTTGT+TGG | - | chr4.4:3824077-3824096 | MS.gene20159:intron | 30.0% | |
| TGTGAACTCTAAACTAACTA+AGG | + | chr4.4:3825002-3825021 | None:intergenic | 30.0% | |
| TGTTGAATTGAAACATCAGA+AGG | - | chr4.4:3825384-3825403 | MS.gene20159:intron | 30.0% | |
| TGTTGAATTGAAACATCAGA+TGG | - | chr4.4:3825384-3825403 | MS.gene20159:intron | 30.0% | |
| ! | ATACCACTATAGCATTTTAG+GGG | - | chr4.4:3824839-3824858 | MS.gene20159:intron | 30.0% |
| ! | ATTTTCTCCCTTGTCAATAA+AGG | + | chr4.4:3824183-3824202 | None:intergenic | 30.0% |
| ! | TTTTCTCCCTTGTCAATAAA+GGG | + | chr4.4:3824182-3824201 | None:intergenic | 30.0% |
| AATATGACACTGTTGCATAG+TGG | - | chr4.4:3825232-3825251 | MS.gene20159:intron | 35.0% | |
| AATCCCCTAAAATGCTATAG+TGG | + | chr4.4:3824845-3824864 | None:intergenic | 35.0% | |
| ACACTGTGAACTCTAAACTA+AGG | + | chr4.4:3825006-3825025 | None:intergenic | 35.0% | |
| AGCCAATTACAGTGAATTTG+CGG | - | chr4.4:3824481-3824500 | MS.gene20159:intron | 35.0% | |
| ATAGTGGTATATCGAATAGC+GGG | + | chr4.4:3824829-3824848 | None:intergenic | 35.0% | |
| ATCACATGAACCCTAATTCA+TGG | + | chr4.4:3825336-3825355 | None:intergenic | 35.0% | |
| CACTGTGAACTCTAAACTAA+GGG | + | chr4.4:3825005-3825024 | None:intergenic | 35.0% | |
| CCATTAACTTTGTAGTTCAG+GGG | + | chr4.4:3824274-3824293 | None:intergenic | 35.0% | |
| GAAGAACAAGGAATACATTC+AGG | - | chr4.4:3823934-3823953 | MS.gene20159:CDS | 35.0% | |
| GACCATTAACTTTGTAGTTC+AGG | + | chr4.4:3824276-3824295 | None:intergenic | 35.0% | |
| GATTTGAAGATTGAAGATGC+AGG | - | chr4.4:3825660-3825679 | MS.gene20159:CDS | 35.0% | |
| TATTGATCAGAATTCCCATG+TGG | - | chr4.4:3825445-3825464 | MS.gene20159:intron | 35.0% | |
| TGTTGGATATCATAGAGTTC+AGG | - | chr4.4:3825310-3825329 | MS.gene20159:intron | 35.0% | |
| !! | ACTATAGCATTTTAGGGGAT+TGG | - | chr4.4:3824844-3824863 | MS.gene20159:intron | 35.0% |
| AAAACCATGCATAGCATTCC+AGG | + | chr4.4:3825704-3825723 | None:intergenic | 40.0% | |
| AGAGTTCAGGCCATGAATTA+GGG | - | chr4.4:3825323-3825342 | MS.gene20159:intron | 40.0% | |
| AGTAGAGAAATGCCAACTTC+TGG | - | chr4.4:3823967-3823986 | MS.gene20159:CDS | 40.0% | |
| ATTGACAAGGGAGAAAATCC+TGG | - | chr4.4:3824185-3824204 | MS.gene20159:intron | 40.0% | |
| CCCCTGAACTACAAAGTTAA+TGG | - | chr4.4:3824271-3824290 | MS.gene20159:intron | 40.0% | |
| GCGAGTGTAAAATAGTTGCT+TGG | + | chr4.4:3823993-3824012 | None:intergenic | 40.0% | |
| TAGAGTTCAGGCCATGAATT+AGG | - | chr4.4:3825322-3825341 | MS.gene20159:intron | 40.0% | |
| TCGTTGTGAGTCCTTTCATA+AGG | - | chr4.4:3825599-3825618 | MS.gene20159:CDS | 40.0% | |
| TGAAAGGACTCACAACGATT+TGG | + | chr4.4:3825597-3825616 | None:intergenic | 40.0% | |
| TGATATCCAACACTGAACAC+AGG | + | chr4.4:3825302-3825321 | None:intergenic | 40.0% | |
| TTCCCTCCCTTTATTGACAA+GGG | - | chr4.4:3824173-3824192 | MS.gene20159:intron | 40.0% | |
| TTGTCAATAAAGGGAGGGAA+GGG | + | chr4.4:3824173-3824192 | None:intergenic | 40.0% | |
| TTGTCAATAGCGTGCTATAG+CGG | - | chr4.4:3824372-3824391 | MS.gene20159:intron | 40.0% | |
| ! | TATTGCTGACTGTGGTTTGT+GGG | + | chr4.4:3824447-3824466 | None:intergenic | 40.0% |
| ! | TATTTTACACTCGCCTTGCT+AGG | - | chr4.4:3823999-3824018 | MS.gene20159:CDS | 40.0% |
| !! | TTTTGTGCTGGTCAGATTGT+TGG | - | chr4.4:3825720-3825739 | MS.gene20159:CDS | 40.0% |
| !!! | GCAGGTATTGCTGTTTTGTT+TGG | - | chr4.4:3825678-3825697 | MS.gene20159:CDS | 40.0% |
| !!! | TATTGCTGTTTTGTTTGGCC+TGG | - | chr4.4:3825683-3825702 | MS.gene20159:CDS | 40.0% |
| AACACAGGTGTAGTCAACTG+TGG | + | chr4.4:3825287-3825306 | None:intergenic | 45.0% | |
| AACGTATCCGTGGTAGGATT+TGG | - | chr4.4:3824398-3824417 | MS.gene20159:intron | 45.0% | |
| ACAAGGGAGAAAATCCTGGA+GGG | - | chr4.4:3824189-3824208 | MS.gene20159:intron | 45.0% | |
| ACTACACCTGTGTTCAGTGT+TGG | - | chr4.4:3825293-3825312 | MS.gene20159:intron | 45.0% | |
| AGCAGACACGATAGCAGTTT+GGG | + | chr4.4:3824508-3824527 | None:intergenic | 45.0% | |
| ATAGTTGCTTGGCCAGAAGT+TGG | + | chr4.4:3823982-3824001 | None:intergenic | 45.0% | |
| ATCCGTGGTAGGATTTGGAA+CGG | - | chr4.4:3824403-3824422 | MS.gene20159:intron | 45.0% | |
| CAAACCACAGTCAGCAATAG+TGG | - | chr4.4:3824448-3824467 | MS.gene20159:intron | 45.0% | |
| CTCCCTTGTCAATAAAGGGA+GGG | + | chr4.4:3824178-3824197 | None:intergenic | 45.0% | |
| CTGTGCAATAGTGGCTATAG+CGG | - | chr4.4:3825208-3825227 | MS.gene20159:intron | 45.0% | |
| CTTCCCTCCCTTTATTGACA+AGG | - | chr4.4:3824172-3824191 | MS.gene20159:intron | 45.0% | |
| CTTGTCAATAAAGGGAGGGA+AGG | + | chr4.4:3824174-3824193 | None:intergenic | 45.0% | |
| GGAAGGGGATTTACTTTCAC+AGG | - | chr4.4:3825741-3825760 | MS.gene20159:CDS | 45.0% | |
| GGCCGCAAATTCACTGTAAT+TGG | + | chr4.4:3824486-3824505 | None:intergenic | 45.0% | |
| TAGCAGACACGATAGCAGTT+TGG | + | chr4.4:3824509-3824528 | None:intergenic | 45.0% | |
| TCTCCCTTGTCAATAAAGGG+AGG | + | chr4.4:3824179-3824198 | None:intergenic | 45.0% | |
| TGCTGGTCAGATTGTTGGAA+GGG | - | chr4.4:3825725-3825744 | MS.gene20159:CDS | 45.0% | |
| TGGCATTTCTCTACTGTAGC+AGG | + | chr4.4:3823962-3823981 | None:intergenic | 45.0% | |
| TTCTTACCACAAGCAGCTGT+TGG | - | chr4.4:3823910-3823929 | MS.gene20159:CDS | 45.0% | |
| ! | ATAGCGGAATAACGTATCCG+TGG | - | chr4.4:3824388-3824407 | MS.gene20159:intron | 45.0% |
| ! | CTATTGCTGACTGTGGTTTG+TGG | + | chr4.4:3824448-3824467 | None:intergenic | 45.0% |
| !! | GCCTCTAAAAAGCACCACAT+GGG | + | chr4.4:3825462-3825481 | None:intergenic | 45.0% |
| !! | TCCCATGTGGTGCTTTTTAG+AGG | - | chr4.4:3825458-3825477 | MS.gene20159:intron | 45.0% |
| !! | TGCCTCTAAAAAGCACCACA+TGG | + | chr4.4:3825463-3825482 | None:intergenic | 45.0% |
| !!! | TGCTATGCATGGTTTTGTGC+TGG | - | chr4.4:3825708-3825727 | MS.gene20159:CDS | 45.0% |
| AACTTGAAACCTCACCCTCC+AGG | + | chr4.4:3824206-3824225 | None:intergenic | 50.0% | |
| AAGATGATCGGGTGATCGTG+TGG | - | chr4.4:3824922-3824941 | MS.gene20159:intron | 50.0% | |
| ACCACAGTCAGCAATAGTGG+TGG | - | chr4.4:3824451-3824470 | MS.gene20159:intron | 50.0% | |
| CGGAATAACGTATCCGTGGT+AGG | - | chr4.4:3824392-3824411 | MS.gene20159:intron | 50.0% | |
| CTGCTATCGTGTCTGCTAGT+AGG | - | chr4.4:3824511-3824530 | MS.gene20159:intron | 50.0% | |
| GACAAGGGAGAAAATCCTGG+AGG | - | chr4.4:3824188-3824207 | MS.gene20159:intron | 50.0% | |
| GCAGACACGATAGCAGTTTG+GGG | + | chr4.4:3824507-3824526 | None:intergenic | 50.0% | |
| GCAGCTGTTGGAGAAGAACA+AGG | - | chr4.4:3823922-3823941 | MS.gene20159:CDS | 50.0% | |
| GCTGGTCAGATTGTTGGAAG+GGG | - | chr4.4:3825726-3825745 | MS.gene20159:CDS | 50.0% | |
| GTCCGTTCCAAATCCTACCA+CGG | + | chr4.4:3824408-3824427 | None:intergenic | 50.0% | |
| TCTTCTCCAACAGCTGCTTG+TGG | + | chr4.4:3823919-3823938 | None:intergenic | 50.0% | |
| TTGGCCTGGAATGCTATGCA+TGG | - | chr4.4:3825697-3825716 | MS.gene20159:CDS | 50.0% | |
| ! | ACCACCACTATTGCTGACTG+TGG | + | chr4.4:3824455-3824474 | None:intergenic | 50.0% |
| ! | GGGATTGGTGCATGCTATCT+GGG | - | chr4.4:3824859-3824878 | MS.gene20159:intron | 50.0% |
| ! | GTGCTGGTCAGATTGTTGGA+AGG | - | chr4.4:3825724-3825743 | MS.gene20159:CDS | 50.0% |
| GGAGAAAATCCTGGAGGGTG+AGG | - | chr4.4:3824194-3824213 | MS.gene20159:intron | 55.0% | |
| ! | AGGATTTGGAACGGACCGCA+CGG | - | chr4.4:3824412-3824431 | MS.gene20159:intron | 55.0% |
| ! | GGGGATTGGTGCATGCTATC+TGG | - | chr4.4:3824858-3824877 | MS.gene20159:intron | 55.0% |
| ! | AACGGACCGCACGGTGCTTT+TGG | - | chr4.4:3824421-3824440 | MS.gene20159:intron | 60.0% |
| !! | TGTGGGCCAAAAGCACCGTG+CGG | + | chr4.4:3824430-3824449 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 3823842 | 3825776 | 3823842 | ID=MS.gene20159 |
| chr4.4 | mRNA | 3823842 | 3825776 | 3823842 | ID=MS.gene20159.t1;Parent=MS.gene20159 |
| chr4.4 | exon | 3825598 | 3825776 | 3825598 | ID=MS.gene20159.t1.exon1;Parent=MS.gene20159.t1 |
| chr4.4 | CDS | 3825598 | 3825776 | 3825598 | ID=cds.MS.gene20159.t1;Parent=MS.gene20159.t1 |
| chr4.4 | exon | 3823842 | 3824028 | 3823842 | ID=MS.gene20159.t1.exon2;Parent=MS.gene20159.t1 |
| chr4.4 | CDS | 3823842 | 3824028 | 3823842 | ID=cds.MS.gene20159.t1;Parent=MS.gene20159.t1 |
| Gene Sequence |
| Protein sequence |
