Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20201.t1 | KEH21283.1 | 94.2 | 86 | 2 | 1 | 1 | 83 | 1 | 86 | 3.20E-17 | 97.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20201.t1 | A0A072TWM2 | 94.2 | 86 | 2 | 1 | 1 | 83 | 1 | 86 | 2.3e-17 | 97.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051033 | MS.gene20201 | -0.813127 | 3.00E-51 | -1.69E-46 |
| MS.gene057130 | MS.gene20201 | 0.834594 | 2.74E-56 | -1.69E-46 |
| MS.gene06118 | MS.gene20201 | 0.92124 | 4.61E-88 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20201.t1 | MTR_8g099965 | 94.186 | 86 | 2 | 1 | 1 | 83 | 1 | 86 | 4.33e-51 | 155 |
| MS.gene20201.t1 | MTR_8g027380 | 61.905 | 42 | 16 | 0 | 3 | 44 | 8 | 49 | 5.93e-13 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20201.t1 | AT4G10810 | 62.500 | 48 | 18 | 0 | 4 | 51 | 16 | 63 | 2.95e-13 | 59.3 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACTTCTCTTTAGATATC+AGG | 0.264418 | 4.4:+4408690 | None:intergenic |
| TTCCGCTTTCTTCTCTTCCT+TGG | 0.296008 | 4.4:-4408633 | MS.gene20201:CDS |
| TCATCGTCTTCGTCGTTATC+AGG | 0.344564 | 4.4:+4408572 | None:intergenic |
| GGAGGTTGGTGATATCGTTT+GGG | 0.357316 | 4.4:+4408486 | None:intergenic |
| GGGAGGTTGGTGATATCGTT+TGG | 0.364836 | 4.4:+4408485 | None:intergenic |
| GATCAGATGGCAACCTGGGT+GGG | 0.426814 | 4.4:-4408668 | MS.gene20201:CDS |
| GGGGTCTTAACGGAGGGATC+AGG | 0.428723 | 4.4:+4408506 | None:intergenic |
| GGTGGTGGTGGTGACGGAGT+CGG | 0.448510 | 4.4:+4408544 | None:intergenic |
| ATCAGATGGCAACCTGGGTG+GGG | 0.454986 | 4.4:-4408667 | MS.gene20201:CDS |
| CCTGATATCTAAAGAGAAGT+TGG | 0.455522 | 4.4:-4408690 | MS.gene20201:CDS |
| GTTGACGGTGGTGGTGGTGG+TGG | 0.455838 | 4.4:+4408532 | None:intergenic |
| ATTGTTGACGGTGGTGGTGG+TGG | 0.457641 | 4.4:+4408529 | None:intergenic |
| ATCAGGATTGTTGACGGTGG+TGG | 0.467123 | 4.4:+4408523 | None:intergenic |
| GATATCGTTTGGGGTCTTAA+CGG | 0.478557 | 4.4:+4408496 | None:intergenic |
| AGGATTGTTGACGGTGGTGG+TGG | 0.495328 | 4.4:+4408526 | None:intergenic |
| TAAAGAGAAGTTGGATCAGA+TGG | 0.515604 | 4.4:-4408681 | MS.gene20201:CDS |
| AGAGAAGAAAGCGGAAGAGA+CGG | 0.533991 | 4.4:+4408640 | None:intergenic |
| AGTTGGATCAGATGGCAACC+TGG | 0.543370 | 4.4:-4408673 | MS.gene20201:CDS |
| GACGCAAGAAAAGCGTTCCA+AGG | 0.548791 | 4.4:+4408616 | None:intergenic |
| GGATCAGATGGCAACCTGGG+TGG | 0.570514 | 4.4:-4408669 | MS.gene20201:CDS |
| GAAGAAAGCGGAAGAGACGG+TGG | 0.578991 | 4.4:+4408643 | None:intergenic |
| GTTGGATCAGATGGCAACCT+GGG | 0.581479 | 4.4:-4408672 | MS.gene20201:CDS |
| GGAGGGATCAGGATTGTTGA+CGG | 0.583608 | 4.4:+4408517 | None:intergenic |
| TCGTTTGGGGTCTTAACGGA+GGG | 0.589276 | 4.4:+4408500 | None:intergenic |
| ATCGTTTGGGGTCTTAACGG+AGG | 0.613773 | 4.4:+4408499 | None:intergenic |
| AGAGACGGTGGACCCCACCC+AGG | 0.649497 | 4.4:+4408655 | None:intergenic |
| TCGTCTTCGTCGTTATCAGG+AGG | 0.685685 | 4.4:+4408575 | None:intergenic |
| TTCCAAGGAAGAGAAGAAAG+CGG | 0.690072 | 4.4:+4408631 | None:intergenic |
| GTTATCAGGAGGATCAGAAG+TGG | 0.695959 | 4.4:+4408586 | None:intergenic |
| GAGGTTGGTGATATCGTTTG+GGG | 0.715129 | 4.4:+4408487 | None:intergenic |
| GGGATCAGGATTGTTGACGG+TGG | 0.721497 | 4.4:+4408520 | None:intergenic |
| TCGACCACGATGGAGAACAA+CGG | 0.739992 | 4.4:-4408716 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CCAACTTCTCTTTAGATATC+AGG | + | chr4.4:4408498-4408517 | None:intergenic | 35.0% | |
| CCTGATATCTAAAGAGAAGT+TGG | - | chr4.4:4408495-4408514 | MS.gene20201:CDS | 35.0% | |
| TAAAGAGAAGTTGGATCAGA+TGG | - | chr4.4:4408504-4408523 | MS.gene20201:CDS | 35.0% | |
| TTCCAAGGAAGAGAAGAAAG+CGG | + | chr4.4:4408557-4408576 | None:intergenic | 40.0% | |
| ! | GATATCGTTTGGGGTCTTAA+CGG | + | chr4.4:4408692-4408711 | None:intergenic | 40.0% |
| AGAGAAGAAAGCGGAAGAGA+CGG | + | chr4.4:4408548-4408567 | None:intergenic | 45.0% | |
| GTTATCAGGAGGATCAGAAG+TGG | + | chr4.4:4408602-4408621 | None:intergenic | 45.0% | |
| TCATCGTCTTCGTCGTTATC+AGG | + | chr4.4:4408616-4408635 | None:intergenic | 45.0% | |
| TTCCGCTTTCTTCTCTTCCT+TGG | - | chr4.4:4408552-4408571 | MS.gene20201:CDS | 45.0% | |
| !! | GAGGTTGGTGATATCGTTTG+GGG | + | chr4.4:4408701-4408720 | None:intergenic | 45.0% |
| !! | GGAGGTTGGTGATATCGTTT+GGG | + | chr4.4:4408702-4408721 | None:intergenic | 45.0% |
| AGTTGGATCAGATGGCAACC+TGG | - | chr4.4:4408512-4408531 | MS.gene20201:CDS | 50.0% | |
| ATCAGGATTGTTGACGGTGG+TGG | + | chr4.4:4408665-4408684 | None:intergenic | 50.0% | |
| GGAGGGATCAGGATTGTTGA+CGG | + | chr4.4:4408671-4408690 | None:intergenic | 50.0% | |
| GTTGGATCAGATGGCAACCT+GGG | - | chr4.4:4408513-4408532 | MS.gene20201:CDS | 50.0% | |
| TCGTCTTCGTCGTTATCAGG+AGG | + | chr4.4:4408613-4408632 | None:intergenic | 50.0% | |
| ! | ATCGTTTGGGGTCTTAACGG+AGG | + | chr4.4:4408689-4408708 | None:intergenic | 50.0% |
| ! | TCGTTTGGGGTCTTAACGGA+GGG | + | chr4.4:4408688-4408707 | None:intergenic | 50.0% |
| !! | GACGCAAGAAAAGCGTTCCA+AGG | + | chr4.4:4408572-4408591 | None:intergenic | 50.0% |
| !! | GGGAGGTTGGTGATATCGTT+TGG | + | chr4.4:4408703-4408722 | None:intergenic | 50.0% |
| ATCAGATGGCAACCTGGGTG+GGG | - | chr4.4:4408518-4408537 | MS.gene20201:CDS | 55.0% | |
| GAAGAAAGCGGAAGAGACGG+TGG | + | chr4.4:4408545-4408564 | None:intergenic | 55.0% | |
| GATCAGATGGCAACCTGGGT+GGG | - | chr4.4:4408517-4408536 | MS.gene20201:CDS | 55.0% | |
| GGGATCAGGATTGTTGACGG+TGG | + | chr4.4:4408668-4408687 | None:intergenic | 55.0% | |
| ! | AGGATTGTTGACGGTGGTGG+TGG | + | chr4.4:4408662-4408681 | None:intergenic | 55.0% |
| !! | ATTGTTGACGGTGGTGGTGG+TGG | + | chr4.4:4408659-4408678 | None:intergenic | 55.0% |
| GGATCAGATGGCAACCTGGG+TGG | - | chr4.4:4408516-4408535 | MS.gene20201:CDS | 60.0% | |
| GGGGTCTTAACGGAGGGATC+AGG | + | chr4.4:4408682-4408701 | None:intergenic | 60.0% | |
| !! | GGTGGTGGTGGTGACGGAGT+CGG | + | chr4.4:4408644-4408663 | None:intergenic | 65.0% |
| !! | GGTGGTGGTGGTGGTGGTGA+CGG | + | chr4.4:4408650-4408669 | None:intergenic | 65.0% |
| !! | GTTGACGGTGGTGGTGGTGG+TGG | + | chr4.4:4408656-4408675 | None:intergenic | 65.0% |
| AGAGACGGTGGACCCCACCC+AGG | + | chr4.4:4408533-4408552 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 4408478 | 4408729 | 4408478 | ID=MS.gene20201 |
| chr4.4 | mRNA | 4408478 | 4408729 | 4408478 | ID=MS.gene20201.t1;Parent=MS.gene20201 |
| chr4.4 | exon | 4408478 | 4408729 | 4408478 | ID=MS.gene20201.t1.exon1;Parent=MS.gene20201.t1 |
| chr4.4 | CDS | 4408478 | 4408729 | 4408478 | ID=cds.MS.gene20201.t1;Parent=MS.gene20201.t1 |
| Gene Sequence |
| Protein sequence |
