Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057130.t1 | XP_013461435.1 | 98.3 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 5.90E-27 | 129.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057130.t1 | A0A072VBC0 | 98.3 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 4.3e-27 | 129.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05029 | MS.gene057130 | 0.8418 | 3.82E-58 | -1.69E-46 |
| MS.gene051033 | MS.gene057130 | -0.802576 | 5.25E-49 | -1.69E-46 |
| MS.gene057130 | MS.gene06118 | 0.840869 | 6.71E-58 | -1.69E-46 |
| MS.gene057130 | MS.gene061580 | 0.807486 | 4.94E-50 | -1.69E-46 |
| MS.gene057130 | MS.gene067571 | 0.82859 | 8.26E-55 | -1.69E-46 |
| MS.gene057130 | MS.gene06892 | 0.982075 | 3.61E-154 | -1.69E-46 |
| MS.gene057130 | MS.gene069962 | 0.829109 | 6.19E-55 | -1.69E-46 |
| MS.gene057130 | MS.gene071246 | -0.805435 | 1.34E-49 | -1.69E-46 |
| MS.gene057130 | MS.gene073207 | 0.801599 | 8.34E-49 | -1.69E-46 |
| MS.gene057130 | MS.gene07417 | 0.800492 | 1.41E-48 | -1.69E-46 |
| MS.gene057130 | MS.gene09056 | 0.804083 | 2.56E-49 | -1.69E-46 |
| MS.gene057130 | MS.gene20201 | 0.834594 | 2.74E-56 | -1.69E-46 |
| MS.gene057130 | MS.gene31405 | 0.81501 | 1.15E-51 | -1.69E-46 |
| MS.gene057130 | MS.gene32708 | 0.835543 | 1.58E-56 | -1.69E-46 |
| MS.gene057130 | MS.gene33829 | 0.80768 | 4.49E-50 | -1.69E-46 |
| MS.gene057130 | MS.gene34507 | -0.812716 | 3.69E-51 | -1.69E-46 |
| MS.gene057130 | MS.gene36490 | 0.816051 | 6.75E-52 | -1.69E-46 |
| MS.gene057130 | MS.gene42671 | 0.847931 | 8.46E-60 | -1.69E-46 |
| MS.gene057130 | MS.gene55837 | 0.832462 | 9.32E-56 | -1.69E-46 |
| MS.gene057130 | MS.gene66461 | 0.831386 | 1.72E-55 | -1.69E-46 |
| MS.gene057130 | MS.gene74608 | 0.831022 | 2.11E-55 | -1.69E-46 |
| MS.gene057130 | MS.gene80177 | 0.815871 | 7.40E-52 | -1.69E-46 |
| MS.gene057130 | MS.gene83528 | -0.826153 | 3.17E-54 | -1.69E-46 |
| MS.gene057130 | MS.gene86991 | 0.829815 | 4.17E-55 | -1.69E-46 |
| MS.gene057130 | MS.gene90372 | -0.826663 | 2.40E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057130.t1 | MTR_3g491900 | 96.429 | 84 | 3 | 0 | 1 | 84 | 1 | 84 | 8.08e-56 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057130.t1 | AT4G10810 | 68.750 | 32 | 10 | 0 | 17 | 48 | 29 | 60 | 7.48e-11 | 53.1 |
Find 31 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGGCTTTCTTTGCATCTT+TGG | 0.266381 | 3.1:+73754306 | MS.gene057130:CDS |
| GGAGGAGGAGGCTGTGGTTG+AGG | 0.362552 | 3.1:-73754430 | None:intergenic |
| TACCCTTCACCCTCTGGTCC+TGG | 0.409818 | 3.1:-73754241 | None:intergenic |
| CTCTGGTCCTGGTCTCTCCA+TGG | 0.416814 | 3.1:-73754230 | None:intergenic |
| CAGAGGGTGAAGGGTATTGC+TGG | 0.426530 | 3.1:+73754248 | MS.gene057130:CDS |
| CAGCAATACCCTTCACCCTC+TGG | 0.445917 | 3.1:-73754247 | None:intergenic |
| AGGATGAGAAGAGGATCGTC+AGG | 0.481227 | 3.1:-73754367 | None:intergenic |
| TCGGAAGGAGGAGGAGGCTG+TGG | 0.506854 | 3.1:-73754436 | None:intergenic |
| CTTTCTTTGCATCTTTGGAA+CGG | 0.508553 | 3.1:+73754311 | MS.gene057130:CDS |
| ACGGAATGGCGAGCTGGATA+GGG | 0.508733 | 3.1:+73754272 | MS.gene057130:CDS |
| AACGGAATGGCGAGCTGGAT+AGG | 0.517911 | 3.1:+73754271 | MS.gene057130:CDS |
| AAGAGGATCGTCAGGATCAT+CGG | 0.521870 | 3.1:-73754359 | None:intergenic |
| TTGTTGTCGGAAGGAGGAGG+AGG | 0.522839 | 3.1:-73754442 | None:intergenic |
| ATCGTCAGGATCATCGGAGG+TGG | 0.525533 | 3.1:-73754353 | None:intergenic |
| GACCAGGACCAGAGGGTGAA+GGG | 0.537012 | 3.1:+73754239 | MS.gene057130:CDS |
| AGATTCATCGGAGAATCTGT+TGG | 0.545815 | 3.1:-73754401 | None:intergenic |
| AGACCAGGACCAGAGGGTGA+AGG | 0.547532 | 3.1:+73754238 | MS.gene057130:CDS |
| GGAGAATCTGTTGGAATCAG+AGG | 0.559994 | 3.1:-73754392 | None:intergenic |
| GCTGGAACGGAATGGCGAGC+TGG | 0.561719 | 3.1:+73754266 | MS.gene057130:CDS |
| GGTGAAGGGTATTGCTGGAA+CGG | 0.573724 | 3.1:+73754253 | MS.gene057130:CDS |
| GAATCATCCATGGAGAGACC+AGG | 0.575340 | 3.1:+73754223 | None:intergenic |
| ATCTGTTGGAATCAGAGGGA+AGG | 0.586267 | 3.1:-73754387 | None:intergenic |
| TTGAGGATGAGAAGATTCAT+CGG | 0.597564 | 3.1:-73754413 | None:intergenic |
| ATGGAGAGACCAGGACCAGA+GGG | 0.603001 | 3.1:+73754232 | MS.gene057130:CDS |
| TCAGAGGGAAGGATGAGAAG+AGG | 0.603416 | 3.1:-73754376 | None:intergenic |
| AGGGTATTGCTGGAACGGAA+TGG | 0.614647 | 3.1:+73754258 | MS.gene057130:CDS |
| GAGAATCTGTTGGAATCAGA+GGG | 0.616835 | 3.1:-73754391 | None:intergenic |
| GATAGGGATGAATCTTGCAA+CGG | 0.636350 | 3.1:+73754288 | MS.gene057130:CDS |
| CTACCCTAAAACTCAAACAG+CGG | 0.639714 | 3.1:-73754476 | None:intergenic |
| CATGGAGAGACCAGGACCAG+AGG | 0.641019 | 3.1:+73754231 | None:intergenic |
| AGGATCGTCAGGATCATCGG+AGG | 0.716513 | 3.1:-73754356 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGAGGATGAGAAGATTCAT+CGG | - | chr3.1:73754416-73754435 | None:intergenic | 35.0% | |
| ! | CTTTCTTTGCATCTTTGGAA+CGG | + | chr3.1:73754311-73754330 | MS.gene057130:CDS | 35.0% |
| !!! | TTGTTGTTTTTGTTGTCGGA+AGG | - | chr3.1:73754454-73754473 | None:intergenic | 35.0% |
| AACGGCTTTCTTTGCATCTT+TGG | + | chr3.1:73754306-73754325 | MS.gene057130:CDS | 40.0% | |
| AGATTCATCGGAGAATCTGT+TGG | - | chr3.1:73754404-73754423 | None:intergenic | 40.0% | |
| GAGAATCTGTTGGAATCAGA+GGG | - | chr3.1:73754394-73754413 | None:intergenic | 40.0% | |
| GATAGGGATGAATCTTGCAA+CGG | + | chr3.1:73754288-73754307 | MS.gene057130:CDS | 40.0% | |
| !!! | GGCGTTGTTGTTTTTGTTGT+CGG | - | chr3.1:73754458-73754477 | None:intergenic | 40.0% |
| !!! | TTGTTTTTGTTGTCGGAAGG+AGG | - | chr3.1:73754451-73754470 | None:intergenic | 40.0% |
| AAGAGGATCGTCAGGATCAT+CGG | - | chr3.1:73754362-73754381 | None:intergenic | 45.0% | |
| ATCTGTTGGAATCAGAGGGA+AGG | - | chr3.1:73754390-73754409 | None:intergenic | 45.0% | |
| GGAGAATCTGTTGGAATCAG+AGG | - | chr3.1:73754395-73754414 | None:intergenic | 45.0% | |
| !! | TTTTTGTTGTCGGAAGGAGG+AGG | - | chr3.1:73754448-73754467 | None:intergenic | 45.0% |
| AGGATGAGAAGAGGATCGTC+AGG | - | chr3.1:73754370-73754389 | None:intergenic | 50.0% | |
| TCAGAGGGAAGGATGAGAAG+AGG | - | chr3.1:73754379-73754398 | None:intergenic | 50.0% | |
| !! | AGGGTATTGCTGGAACGGAA+TGG | + | chr3.1:73754258-73754277 | MS.gene057130:CDS | 50.0% |
| !! | GGTGAAGGGTATTGCTGGAA+CGG | + | chr3.1:73754253-73754272 | MS.gene057130:CDS | 50.0% |
| AACGGAATGGCGAGCTGGAT+AGG | + | chr3.1:73754271-73754290 | MS.gene057130:CDS | 55.0% | |
| ACGGAATGGCGAGCTGGATA+GGG | + | chr3.1:73754272-73754291 | MS.gene057130:CDS | 55.0% | |
| AGGATCGTCAGGATCATCGG+AGG | - | chr3.1:73754359-73754378 | None:intergenic | 55.0% | |
| ATCGTCAGGATCATCGGAGG+TGG | - | chr3.1:73754356-73754375 | None:intergenic | 55.0% | |
| ATGGAGAGACCAGGACCAGA+GGG | + | chr3.1:73754232-73754251 | MS.gene057130:CDS | 55.0% | |
| CAGCAATACCCTTCACCCTC+TGG | - | chr3.1:73754250-73754269 | None:intergenic | 55.0% | |
| TTGTTGTCGGAAGGAGGAGG+AGG | - | chr3.1:73754445-73754464 | None:intergenic | 55.0% | |
| !! | CAGAGGGTGAAGGGTATTGC+TGG | + | chr3.1:73754248-73754267 | MS.gene057130:CDS | 55.0% |
| AGACCAGGACCAGAGGGTGA+AGG | + | chr3.1:73754238-73754257 | MS.gene057130:CDS | 60.0% | |
| GACCAGGACCAGAGGGTGAA+GGG | + | chr3.1:73754239-73754258 | MS.gene057130:CDS | 60.0% | |
| TACCCTTCACCCTCTGGTCC+TGG | - | chr3.1:73754244-73754263 | None:intergenic | 60.0% | |
| GCTGGAACGGAATGGCGAGC+TGG | + | chr3.1:73754266-73754285 | MS.gene057130:CDS | 65.0% | |
| GGAGGAGGAGGCTGTGGTTG+AGG | - | chr3.1:73754433-73754452 | None:intergenic | 65.0% | |
| TCGGAAGGAGGAGGAGGCTG+TGG | - | chr3.1:73754439-73754458 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 73754232 | 73754486 | 73754232 | ID=MS.gene057130 |
| chr3.1 | mRNA | 73754232 | 73754486 | 73754232 | ID=MS.gene057130.t1;Parent=MS.gene057130 |
| chr3.1 | exon | 73754232 | 73754486 | 73754232 | ID=MS.gene057130.t1.exon1;Parent=MS.gene057130.t1 |
| chr3.1 | CDS | 73754232 | 73754486 | 73754232 | ID=cds.MS.gene057130.t1;Parent=MS.gene057130.t1 |
| Gene Sequence |
| Protein sequence |
