AlfalfaGEDB | MS.gene20440

M. sativa cultivar XinJiangDaYe / MS.gene20440

KEGG: K18980
GO: GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0008270 GO:0016491 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0055114
PFAM: PF08240.15 PF13602.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20440.t1	XP_003618487.1	96.2	320	12	0	1	320	1	320	4.80E-170	607.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20440.t1	Q84V25	72.7	319	87	0	2	320	4	322	3.4e-132	472.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20440.t1	G7KKH6	96.2	320	12	0	1	320	1	320	3.5e-170	607.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051279	MS.gene20440	0.801834	7.47E-49	-1.69E-46
MS.gene053144	MS.gene20440	0.800948	1.13E-48	-1.69E-46
MS.gene053536	MS.gene20440	0.810135	1.34E-50	-1.69E-46
MS.gene053538	MS.gene20440	0.808407	3.15E-50	-1.69E-46
MS.gene055537	MS.gene20440	0.81386	2.07E-51	-1.69E-46
MS.gene056169	MS.gene20440	0.805305	1.42E-49	-1.69E-46
MS.gene05894	MS.gene20440	0.816805	4.58E-52	-1.69E-46
MS.gene059424	MS.gene20440	0.804806	1.81E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20440.t1	MTR_6g012170	96.250	320	12	0	1	320	1	320	0.0	586
MS.gene20440.t1	MTR_6g012160	92.177	294	23	0	1	294	1	294	0.0	518
MS.gene20440.t1	MTR_8g064610	67.834	314	100	1	7	320	72	384	7.94e-154	436
MS.gene20440.t1	MTR_7g034395	71.197	309	89	0	12	320	9	317	6.25e-145	411
MS.gene20440.t1	MTR_8g035630	46.302	311	161	5	12	320	5	311	1.25e-86	264
MS.gene20440.t1	MTR_8g035880	46.906	307	157	5	12	316	5	307	4.82e-86	262
MS.gene20440.t1	MTR_6g012140	85.106	141	21	0	179	319	3	143	1.40e-82	246
MS.gene20440.t1	MTR_1319s0010	71.028	107	31	0	73	179	3	109	2.33e-41	140
MS.gene20440.t1	MTR_4g036310	35.562	329	174	12	16	317	11	328	9.29e-37	134
MS.gene20440.t1	MTR_7g055743	34.132	334	169	12	13	317	57	368	9.23e-36	132

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20440.t1	AT1G23740	63.492	315	114	1	6	320	73	386	3.28e-141	404
MS.gene20440.t1	AT4G13010	31.928	332	184	10	16	318	11	329	1.48e-32	123
MS.gene20440.t1	AT3G15090	33.463	257	157	8	1	251	22	270	2.36e-30	118
MS.gene20440.t1	AT4G21580	28.614	339	193	12	12	317	2	324	2.17e-25	103
MS.gene20440.t1	AT4G21580	28.107	338	190	12	12	317	2	318	1.94e-24	101
MS.gene20440.t1	AT3G56460	29.487	312	173	10	39	316	38	336	7.42e-21	92.0
MS.gene20440.t1	AT1G49670	26.625	323	182	11	35	320	316	620	5.16e-18	85.1
MS.gene20440.t1	AT1G49670	25.581	344	182	11	35	320	316	643	3.82e-17	82.4
MS.gene20440.t1	AT3G45770	27.551	294	179	10	11	284	45	324	2.12e-15	76.6
MS.gene20440.t1	AT5G61510	29.954	217	133	8	24	233	96	300	3.18e-12	67.4

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Find 66 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGAAAGGGACTCTTA+GGG	0.647115	6.3:+6581786	None:intergenic
AAATCTCTACTTGTTCTTGC+AGG	0.339592	6.3:-6582738	MS.gene20440:CDS
AAGCAGTAATTATTGCTAAA+GGG	0.461547	6.3:+6582796	None:intergenic
AAGGTTGGAGATGAAGTTTA+TGG	0.348171	6.3:-6582945	MS.gene20440:CDS
ACACCAGCTACATCATATCC+TGG	0.567906	6.3:+6583002	None:intergenic
ACAGCCTGAGAAAATGGAAA+GGG	0.579911	6.3:+6581776	None:intergenic
ACTAACTGAAAAGTTTGATG+TGG	0.682063	6.3:-6582107	MS.gene20440:CDS
ACTTGATCTTCTCTAGGGTG+TGG	0.546580	6.3:+6583523	None:intergenic
AGAAGATCAAGTGCTCATCA+AGG	0.612803	6.3:-6583512	MS.gene20440:CDS
AGACCAAGTGAAGAAATTTA+AGG	0.233255	6.3:-6582964	MS.gene20440:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTGTTTGGAGAATCTGTT+TGG	+	chr6.3:6582975-6582994	None:intergenic	30.0%
ACTAACTGAAAAGTTTGATG+TGG	-	chr6.3:6583227-6583246	MS.gene20440:intron	30.0%
AGACCAAGTGAAGAAATTTA+AGG	-	chr6.3:6582370-6582389	MS.gene20440:intron	30.0%
AGAGAAATTGAAACCTTACT+TGG	-	chr6.3:6583497-6583516	MS.gene20440:CDS	30.0%
CAACCTTAAATTTCTTCACT+TGG	+	chr6.3:6582376-6582395	None:intergenic	30.0%
CTTGGCAATTGATTATACAA+AGG	-	chr6.3:6583191-6583210	MS.gene20440:intron	30.0%
GCAATAATTACTGCTTATCA+AGG	-	chr6.3:6582545-6582564	MS.gene20440:intron	30.0%
TAAGTTAAGGACGAATTGTT+TGG	+	chr6.3:6582988-6583007	None:intergenic	30.0%
TTCTGGTCAATCTTTAGTTA+AGG	+	chr6.3:6582813-6582832	None:intergenic	30.0%
TTGAAACCTTACTTGGAAAA+TGG	-	chr6.3:6583504-6583523	MS.gene20440:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	6581709	6583647	6581709	ID=MS.gene20440
chr6.3	mRNA	6581709	6583647	6581709	ID=MS.gene20440.t1;Parent=MS.gene20440
chr6.3	exon	6583435	6583647	6583435	ID=MS.gene20440.t1.exon1;Parent=MS.gene20440.t1
chr6.3	CDS	6583435	6583647	6583435	ID=cds.MS.gene20440.t1;Parent=MS.gene20440.t1
chr6.3	exon	6582707	6583030	6582707	ID=MS.gene20440.t1.exon2;Parent=MS.gene20440.t1
chr6.3	CDS	6582707	6583030	6582707	ID=cds.MS.gene20440.t1;Parent=MS.gene20440.t1
chr6.3	exon	6582092	6582251	6582092	ID=MS.gene20440.t1.exon3;Parent=MS.gene20440.t1
chr6.3	CDS	6582092	6582251	6582092	ID=cds.MS.gene20440.t1;Parent=MS.gene20440.t1
chr6.3	exon	6581709	6581974	6581709	ID=MS.gene20440.t1.exon4;Parent=MS.gene20440.t1
chr6.3	CDS	6581709	6581974	6581709	ID=cds.MS.gene20440.t1;Parent=MS.gene20440.t1

Gene Sequence

>MS.gene20440

ATGGCAAGCACACATAGTATTCCATCTCATACAAAAGCTTGGGTTTACTCTCAATATGGTAACATAGAAGAAATTCTAAAGTTTGATCCTAATGTCCGTACACCACACCCTAGAGAAGATCAAGTGCTCATCAAGGTTGTAGCTGCTGCCCTTAATCCTGTTGATATTAAGAGGGCTCTTGGTCATTTCAAGGACATTGACTCTCCTTTGCCAGTAAGATTGCAATCACCATCTATGACTTAAATTTCAAGAGTATCAGTACACCTCAATTTGCGAGTGTACCGTAGAAGTTTTCATTTATATTTTTAGTCCTGTAAAAAAAATTCTTTGTTTATAGCCGTTGCAAACATATAATGTTTTTGCTTTTGATTCTATTTGTTATCTTAATTCATATGCACCGTTAGTGTAATTAAATAATTTTTACACCGGCAATCTAATGCAATCAGGCAGATCATTAAAAGCGTCTACTTAGATCTTAATTATTTCTAAAATCACACTTATGATTGGTCGTAGTTTCTTGACGGCGTATAACACCGACAAGGTACCAAAATGAAACTCACAAACATAATGCTAAATAACATGTTTTTTTTCTTCTTCGTTTGCGTGGTGGTGTGAAGACTGTGCCAGGATATGATGTAGCTGGTGTGGTGGTTAGTGTTGGAGACCAAGTGAAGAAATTTAAGGTTGGAGATGAAGTTTATGGTGATATCAATGAGATTACGTTACACAATCCAAAAACTATTGGTACTTTGTCAGAGTATACTGTTGCTGAAGAAAAAGTACTTGCTCACAAACCATCAAATTTGAGTTTTGTTGAAGCTGCTAGTCTCCCTTTAGCAATAATTACTGCTTATCAAGGACTTGAAAGAGTTGAATTTTCTTCTGGTAAATCTCTACTTGTTCTTGCAGGTGCTGGAGGAGTTGGATCTCTTGTTATTCAGGTTTGAATTCTTCTTTCTAAAGATACTCTATACTAGGAATAAATATGATTTTTTTCTTTTTTTGTCTTAATCCCTTGTTCCCAAGAAAAGAGAACCTGGTTAAAGTCGGACTAAAAATTGTTCTAATCAGAAATATAACAGGTTTTCACAAACAATTTGTCCTTAACTAAAGATTGACCAGAAGATAAGTAGTAAATCCGTAATAAATAAGATGAACATTTTTTGTTTTAATTCTGTGTTTCCCGAGAAAAGTGACCTTGGTAATCTGTAGGTTTGACCAGAAGATAAGTAAAGTCCGACTAAAGATTGTTCCAACCAGAAACCAAACAGATTCTCCAAACAATTCGTCCTTAACTTATTCATTGCTCAATATAAGTTGGCTTTTGTAATACATAAAGTATAGAATGTAGTATTTTGTCATCTAATGGAATGCAATTATTTGTTTTGGACTATAGCTAGCCAAGCATGTTTTTGAGGCATCTAGAATTGCAGCTACTGCTAGCACAACAAAACTTGATTTTTTGAGGAAATTGGGAGCAGACTTGGCAATTGATTATACAAAGGAGAATTATGAAGAACTAACTGAAAAGTTTGATGTGGTGTATGATGCAGTAGGTAAGATATTTCTTCTAAATTTAAATCCTTATTAATATGTTGTATTATGTGTAAATTTTCATTGCAAGAAAACAATATAGTTAAAAACCTATTTAATATTTTTTATTTCAAAAATAGGTGATAGTGAGAGGGCAGTGAAGGCTGCCAATGAAGGAGGAAAAGTTGTGACAATATTACCACCTGGAACTCCACCAGCTATCCCTTTCTTACTCAGTTCAGATGGAGCTGTGTTAGAGAAATTGAAACCTTACTTGGAAAATGGCAAAGTGAAACCAATATTGGACCCTAAGAGTCCCTTTCCATTTTCTCAGGCTGTGGAAGCATTTACCTATCTTAATACTAATAGAGCTATTGGGAAAATAGTTATTCACCCCATTCCATGA

Protein sequence

>MS.gene20440.t1

MASTHSIPSHTKAWVYSQYGNIEEILKFDPNVRTPHPREDQVLIKVVAAALNPVDIKRALGHFKDIDSPLPTVPGYDVAGVVVSVGDQVKKFKVGDEVYGDINEITLHNPKTIGTLSEYTVAEEKVLAHKPSNLSFVEAASLPLAIITAYQGLERVEFSSGKSLLVLAGAGGVGSLVIQLAKHVFEASRIAATASTTKLDFLRKLGADLAIDYTKENYEELTEKFDVVYDAVGDSERAVKAANEGGKVVTILPPGTPPAIPFLLSSDGAVLEKLKPYLENGKVKPILDPKSPFPFSQAVEAFTYLNTNRAIGKIVIHPIP