AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20440.t1 MTR_6g012170 96.250 320 12 0 1 320 1 320 0.0 586
MS.gene20440.t1 MTR_6g012160 92.177 294 23 0 1 294 1 294 0.0 518
MS.gene20440.t1 MTR_8g064610 67.834 314 100 1 7 320 72 384 7.94e-154 436
MS.gene20440.t1 MTR_7g034395 71.197 309 89 0 12 320 9 317 6.25e-145 411
MS.gene20440.t1 MTR_8g035630 46.302 311 161 5 12 320 5 311 1.25e-86 264
MS.gene20440.t1 MTR_8g035880 46.906 307 157 5 12 316 5 307 4.82e-86 262
MS.gene20440.t1 MTR_6g012140 85.106 141 21 0 179 319 3 143 1.40e-82 246
MS.gene20440.t1 MTR_1319s0010 71.028 107 31 0 73 179 3 109 2.33e-41 140
MS.gene20440.t1 MTR_4g036310 35.562 329 174 12 16 317 11 328 9.29e-37 134
MS.gene20440.t1 MTR_7g055743 34.132 334 169 12 13 317 57 368 9.23e-36 132
MS.gene20440.t1 MTR_4g032995 32.484 314 175 8 32 318 26 329 7.05e-35 129
MS.gene20440.t1 MTR_4g033205 32.484 314 175 8 32 318 26 329 7.19e-35 129
MS.gene20440.t1 MTR_6g012100 88.710 62 7 0 259 320 8 69 2.85e-33 117
MS.gene20440.t1 MTR_2g078520 30.435 345 193 11 9 316 24 358 2.93e-31 120
MS.gene20440.t1 MTR_4g036260 33.455 275 147 7 70 318 44 308 9.57e-30 115
MS.gene20440.t1 MTR_2g012420 30.368 326 179 13 24 316 13 323 4.55e-27 108
MS.gene20440.t1 MTR_4g050760 29.091 330 178 13 24 316 13 323 8.46e-27 107
MS.gene20440.t1 MTR_2g012380 29.091 330 178 13 24 316 13 323 8.46e-27 107
MS.gene20440.t1 MTR_2g012380 30.165 242 138 9 24 251 13 237 7.53e-23 96.3
MS.gene20440.t1 MTR_4g036170 30.000 290 161 8 54 317 30 303 1.61e-20 90.1
MS.gene20440.t1 MTR_4g086400 26.332 319 182 9 38 316 175 480 1.76e-19 89.0
MS.gene20440.t1 MTR_4g086400 26.332 319 182 9 38 316 315 620 2.06e-19 89.4
MS.gene20440.t1 MTR_4g086410 25.705 319 184 9 38 316 315 620 3.84e-19 88.6
MS.gene20440.t1 MTR_6g039950 57.576 66 28 0 6 71 14 79 1.70e-18 79.0
MS.gene20440.t1 MTR_4g050710 30.085 236 127 10 114 316 16 246 2.12e-15 75.1
MS.gene20440.t1 MTR_2g012420 30.085 236 127 10 114 316 22 252 2.29e-15 75.1
MS.gene20440.t1 MTR_3g093620 28.527 319 178 11 35 316 30 335 7.20e-14 71.6
MS.gene20440.t1 MTR_2g012420 30.208 192 98 8 157 316 4 191 1.96e-13 68.2
MS.gene20440.t1 MTR_8g095220 24.834 302 163 11 6 251 49 342 4.50e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20440.t1 AT1G23740 63.492 315 114 1 6 320 73 386 3.28e-141 404
MS.gene20440.t1 AT4G13010 31.928 332 184 10 16 318 11 329 1.48e-32 123
MS.gene20440.t1 AT3G15090 33.463 257 157 8 1 251 22 270 2.36e-30 118
MS.gene20440.t1 AT4G21580 28.614 339 193 12 12 317 2 324 2.17e-25 103
MS.gene20440.t1 AT4G21580 28.107 338 190 12 12 317 2 318 1.94e-24 101
MS.gene20440.t1 AT3G56460 29.487 312 173 10 39 316 38 336 7.42e-21 92.0
MS.gene20440.t1 AT1G49670 26.625 323 182 11 35 320 316 620 5.16e-18 85.1
MS.gene20440.t1 AT1G49670 25.581 344 182 11 35 320 316 643 3.82e-17 82.4
MS.gene20440.t1 AT3G45770 27.551 294 179 10 11 284 45 324 2.12e-15 76.6
MS.gene20440.t1 AT5G61510 29.954 217 133 8 24 233 96 300 3.18e-12 67.4

Find 66 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTGGATCTCTTGTTATTC+AGG 0.225112 6.3:-6582706 MS.gene20440:intron
TTGAAACCTTACTTGGAAAA+TGG 0.226818 6.3:-6581830 MS.gene20440:CDS
GAAAATGGAAAGGGACTCTT+AGG 0.231928 6.3:+6581785 None:intergenic
AGACCAAGTGAAGAAATTTA+AGG 0.233255 6.3:-6582964 MS.gene20440:CDS
TCTTAATATCAACAGGATTA+AGG 0.239762 6.3:+6583476 None:intergenic
GCTTCCACAGCCTGAGAAAA+TGG 0.285159 6.3:+6581770 None:intergenic
CTTAATACTAATAGAGCTAT+TGG 0.294082 6.3:-6581737 MS.gene20440:CDS
GCTTGGGTTTACTCTCAATA+TGG 0.324385 6.3:-6583589 MS.gene20440:CDS
TCAACAAAACTCAAATTTGA+TGG 0.328083 6.3:+6582831 None:intergenic
GGGATAGCTGGTGGAGTTCC+AGG 0.335884 6.3:+6581890 None:intergenic
AAATCTCTACTTGTTCTTGC+AGG 0.339592 6.3:-6582738 MS.gene20440:CDS
CTACTTGTTCTTGCAGGTGC+TGG 0.342796 6.3:-6582732 MS.gene20440:CDS
AAGGTTGGAGATGAAGTTTA+TGG 0.348171 6.3:-6582945 MS.gene20440:CDS
TGGCAAAGTGAAACCAATAT+TGG 0.356227 6.3:-6581810 MS.gene20440:CDS
CTTAATATCAACAGGATTAA+GGG 0.365726 6.3:+6583477 None:intergenic
TTAATCCTGTTGATATTAAG+AGG 0.366846 6.3:-6583474 MS.gene20440:CDS
GGACTCTTAGGGTCCAATAT+TGG 0.387268 6.3:+6581797 None:intergenic
GGGTGTGGTGTACGGACATT+AGG 0.398446 6.3:+6583538 None:intergenic
AGTATTCATGCTCATGGAAT+GGG 0.401418 6.3:+6581698 None:intergenic
ATGAGCACTTGATCTTCTCT+AGG 0.411783 6.3:+6583517 None:intergenic
CAACCTTAAATTTCTTCACT+TGG 0.416756 6.3:+6582961 None:intergenic
TCCATCTCATACAAAAGCTT+GGG 0.417415 6.3:-6583605 MS.gene20440:CDS
GAGGGCTCTTGGTCATTTCA+AGG 0.427218 6.3:-6583455 MS.gene20440:CDS
CTTGCAGGTGCTGGAGGAGT+TGG 0.436742 6.3:-6582723 MS.gene20440:CDS
TTAATACTAATAGAGCTATT+GGG 0.439543 6.3:-6581736 MS.gene20440:CDS
GAGGAAATTGGGAGCAGACT+TGG 0.439997 6.3:-6582161 MS.gene20440:CDS
TTCCATCTCATACAAAAGCT+TGG 0.442056 6.3:-6583606 MS.gene20440:CDS
AGAGAAATTGAAACCTTACT+TGG 0.445997 6.3:-6581837 MS.gene20440:CDS
TAAGCAGTAATTATTGCTAA+AGG 0.453400 6.3:+6582795 None:intergenic
GCAATAATTACTGCTTATCA+AGG 0.453809 6.3:-6582789 MS.gene20440:CDS
AAGCAGTAATTATTGCTAAA+GGG 0.461547 6.3:+6582796 None:intergenic
AGAGCCCTCTTAATATCAAC+AGG 0.464124 6.3:+6583469 None:intergenic
CTTCTCTAGGGTGTGGTGTA+CGG 0.470092 6.3:+6583530 None:intergenic
CCATCTGAACTGAGTAAGAA+AGG 0.472947 6.3:+6581869 None:intergenic
CCTTTCTTACTCAGTTCAGA+TGG 0.478608 6.3:-6581869 MS.gene20440:CDS
CTTGTTCTTGCAGGTGCTGG+AGG 0.496360 6.3:-6582729 MS.gene20440:CDS
GTTGATATTAAGAGGGCTCT+TGG 0.500705 6.3:-6583466 MS.gene20440:CDS
GCTGGTGTGGTGGTTAGTGT+TGG 0.504039 6.3:-6582987 MS.gene20440:CDS
CTGAGTAAGAAAGGGATAGC+TGG 0.505609 6.3:+6581878 None:intergenic
GAGTATTCATGCTCATGGAA+TGG 0.506512 6.3:+6581697 None:intergenic
AGCTCTATTAGTATTAAGAT+AGG 0.511118 6.3:+6581742 None:intergenic
GCAGTGAAGGCTGCCAATGA+AGG 0.527533 6.3:-6581938 MS.gene20440:CDS
TGAGCACTTGATCTTCTCTA+GGG 0.532012 6.3:+6583518 None:intergenic
CATCTGAACTGAGTAAGAAA+GGG 0.536158 6.3:+6581870 None:intergenic
TGATAGTGAGAGGGCAGTGA+AGG 0.544316 6.3:-6581951 MS.gene20440:CDS
ACTTGATCTTCTCTAGGGTG+TGG 0.546580 6.3:+6583523 None:intergenic
CAAGTGAAGAAATTTAAGGT+TGG 0.546713 6.3:-6582960 MS.gene20440:CDS
ACACCAGCTACATCATATCC+TGG 0.567906 6.3:+6583002 None:intergenic
GTGCCAGGATATGATGTAGC+TGG 0.572325 6.3:-6583005 MS.gene20440:CDS
GGTGGTGTGAAGACTGTGCC+AGG 0.575981 6.3:-6583020 MS.gene20440:intron
ACAGCCTGAGAAAATGGAAA+GGG 0.579911 6.3:+6581776 None:intergenic
TAATCCTGTTGATATTAAGA+GGG 0.592527 6.3:-6583473 MS.gene20440:CDS
GATTGCAATCTTACTGGCAA+AGG 0.597841 6.3:+6583421 None:intergenic
CTTGGCAATTGATTATACAA+AGG 0.607180 6.3:-6582143 MS.gene20440:CDS
CACAGCCTGAGAAAATGGAA+AGG 0.610247 6.3:+6581775 None:intergenic
AGAAGATCAAGTGCTCATCA+AGG 0.612803 6.3:-6583512 MS.gene20440:CDS
AGGATATGATGTAGCTGGTG+TGG 0.622214 6.3:-6583000 MS.gene20440:CDS
GTGAAGGCTGCCAATGAAGG+AGG 0.627985 6.3:-6581935 MS.gene20440:CDS
AGTAAGAAAGGGATAGCTGG+TGG 0.634262 6.3:+6581881 None:intergenic
AAAATGGAAAGGGACTCTTA+GGG 0.647115 6.3:+6581786 None:intergenic
GTATTCATGCTCATGGAATG+GGG 0.667275 6.3:+6581699 None:intergenic
GTTGTGACAATATTACCACC+TGG 0.669517 6.3:-6581908 MS.gene20440:CDS
ATATGATGTAGCTGGTGTGG+TGG 0.678454 6.3:-6582997 MS.gene20440:CDS
ACTAACTGAAAAGTTTGATG+TGG 0.682063 6.3:-6582107 MS.gene20440:CDS
GATGTGGTGTATGATGCAGT+AGG 0.683614 6.3:-6582091 MS.gene20440:intron
ATAGCTGGTGGAGTTCCAGG+TGG 0.759816 6.3:+6581893 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATTTAATTACACTAA+CGG + chr6.3:6582109-6582128 None:intergenic 10.0%
!!! TAATTAAATAATTTTTACAC+CGG - chr6.3:6582115-6582134 MS.gene20440:CDS 10.0%
!! AATATAAATGAAAACTTCTA+CGG + chr6.3:6581993-6582012 None:intergenic 15.0%
!! CATAATACAACATATTAATA+AGG + chr6.3:6583294-6583313 None:intergenic 15.0%
!!! AAACAAAGAATTTTTTTTAC+AGG + chr6.3:6582022-6582041 None:intergenic 15.0%
!!! AATGTTCATCTTATTTATTA+CGG + chr6.3:6582850-6582869 None:intergenic 15.0%
!! AAAACATTATATGTTTGCAA+CGG + chr6.3:6582049-6582068 None:intergenic 20.0%
!! TTAATACTAATAGAGCTATT+GGG - chr6.3:6583598-6583617 MS.gene20440:CDS 20.0%
! AAGCAGTAATTATTGCTAAA+GGG + chr6.3:6582541-6582560 None:intergenic 25.0%
! AGCTCTATTAGTATTAAGAT+AGG + chr6.3:6583595-6583614 None:intergenic 25.0%
! ATTCATTGCTCAATATAAGT+TGG - chr6.3:6583007-6583026 MS.gene20440:CDS 25.0%
! ATTGAGCAATGAATAAGTTA+AGG + chr6.3:6583001-6583020 None:intergenic 25.0%
! CTTAATACTAATAGAGCTAT+TGG - chr6.3:6583597-6583616 MS.gene20440:CDS 25.0%
! CTTAATATCAACAGGATTAA+GGG + chr6.3:6581860-6581879 None:intergenic 25.0%
! CTTGAAATTTAAGTCATAGA+TGG + chr6.3:6581940-6581959 None:intergenic 25.0%
! GTTCTAATCAGAAATATAAC+AGG - chr6.3:6582769-6582788 MS.gene20440:CDS 25.0%
! TAAGCAGTAATTATTGCTAA+AGG + chr6.3:6582542-6582561 None:intergenic 25.0%
! TAATCCTGTTGATATTAAGA+GGG - chr6.3:6581861-6581880 MS.gene20440:CDS 25.0%
! TCAACAAAACTCAAATTTGA+TGG + chr6.3:6582506-6582525 None:intergenic 25.0%
! TCTTAATATCAACAGGATTA+AGG + chr6.3:6581861-6581880 None:intergenic 25.0%
! TTAATCCTGTTGATATTAAG+AGG - chr6.3:6581860-6581879 MS.gene20440:CDS 25.0%
! TTACACAATCCAAAAACTAT+TGG - chr6.3:6582431-6582450 MS.gene20440:intron 25.0%
!! TATGTTTGTGAGTTTCATTT+TGG + chr6.3:6582256-6582275 None:intergenic 25.0%
!! TCTAAAATCACACTTATGAT+TGG - chr6.3:6582193-6582212 MS.gene20440:CDS 25.0%
!! TGGAATGCAATTATTTGTTT+TGG - chr6.3:6583075-6583094 MS.gene20440:intron 25.0%
!! TGTAGTATTTTGTCATCTAA+TGG - chr6.3:6583055-6583074 MS.gene20440:intron 25.0%
!! TTCTAAAGATACTCTATACT+AGG - chr6.3:6582664-6582683 MS.gene20440:intron 25.0%
!!! ACTTGATTTTTTGAGGAAAT+TGG - chr6.3:6583161-6583180 MS.gene20440:intron 25.0%
!!! CAACAAAACTTGATTTTTTG+AGG - chr6.3:6583154-6583173 MS.gene20440:intron 25.0%
!!! CTTGATTTTTTGAGGAAATT+GGG - chr6.3:6583162-6583181 MS.gene20440:intron 25.0%
AATTGTTTGGAGAATCTGTT+TGG + chr6.3:6582975-6582994 None:intergenic 30.0%
ACTAACTGAAAAGTTTGATG+TGG - chr6.3:6583227-6583246 MS.gene20440:intron 30.0%
AGACCAAGTGAAGAAATTTA+AGG - chr6.3:6582370-6582389 MS.gene20440:intron 30.0%
AGAGAAATTGAAACCTTACT+TGG - chr6.3:6583497-6583516 MS.gene20440:CDS 30.0%
CAACCTTAAATTTCTTCACT+TGG + chr6.3:6582376-6582395 None:intergenic 30.0%
CTTGGCAATTGATTATACAA+AGG - chr6.3:6583191-6583210 MS.gene20440:intron 30.0%
GCAATAATTACTGCTTATCA+AGG - chr6.3:6582545-6582564 MS.gene20440:intron 30.0%
TAAGTTAAGGACGAATTGTT+TGG + chr6.3:6582988-6583007 None:intergenic 30.0%
TTCTGGTCAATCTTTAGTTA+AGG + chr6.3:6582813-6582832 None:intergenic 30.0%
TTGAAACCTTACTTGGAAAA+TGG - chr6.3:6583504-6583523 MS.gene20440:CDS 30.0%
! AAAAATAGGTGATAGTGAGA+GGG - chr6.3:6583374-6583393 MS.gene20440:intron 30.0%
! CAAGTGAAGAAATTTAAGGT+TGG - chr6.3:6582374-6582393 MS.gene20440:intron 30.0%
! GAAAGAGTTGAATTTTCTTC+TGG - chr6.3:6582572-6582591 MS.gene20440:intron 30.0%
!! GACAAAGTACCAATAGTTTT+TGG + chr6.3:6582443-6582462 None:intergenic 30.0%
AAAATGGAAAGGGACTCTTA+GGG + chr6.3:6583551-6583570 None:intergenic 35.0%
AAAGAGAACCTGGTTAAAGT+CGG - chr6.3:6582736-6582755 MS.gene20440:CDS 35.0%
AAATCTCTACTTGTTCTTGC+AGG - chr6.3:6582596-6582615 MS.gene20440:intron 35.0%
AAGGTTGGAGATGAAGTTTA+TGG - chr6.3:6582389-6582408 MS.gene20440:intron 35.0%
CATCTGAACTGAGTAAGAAA+GGG + chr6.3:6583467-6583486 None:intergenic 35.0%
CGGATTTACTACTTATCTTC+TGG + chr6.3:6582830-6582849 None:intergenic 35.0%
TCCATCTCATACAAAAGCTT+GGG - chr6.3:6581729-6581748 MS.gene20440:CDS 35.0%
TGGCAAAGTGAAACCAATAT+TGG - chr6.3:6583524-6583543 MS.gene20440:CDS 35.0%
TGGTTGGAACAATCTTTAGT+CGG + chr6.3:6582948-6582967 None:intergenic 35.0%
TTCCATCTCATACAAAAGCT+TGG - chr6.3:6581728-6581747 MS.gene20440:CDS 35.0%
! CAAAAATAGGTGATAGTGAG+AGG - chr6.3:6583373-6583392 MS.gene20440:intron 35.0%
! TAACCAGGTTCTCTTTTCTT+GGG + chr6.3:6582732-6582751 None:intergenic 35.0%
! TTAACCAGGTTCTCTTTTCT+TGG + chr6.3:6582733-6582752 None:intergenic 35.0%
! TTCTCTTTTCTTGGGAACAA+GGG + chr6.3:6582724-6582743 None:intergenic 35.0%
!! AGTTGGATCTCTTGTTATTC+AGG - chr6.3:6582628-6582647 MS.gene20440:intron 35.0%
!! GTTTCATTTTGGTACCTTGT+CGG + chr6.3:6582245-6582264 None:intergenic 35.0%
!! TTTTTAGTCCGACTTTAACC+AGG + chr6.3:6582747-6582766 None:intergenic 35.0%
!!! ACTTTGCCATTTTCCAAGTA+AGG + chr6.3:6583513-6583532 None:intergenic 35.0%
!!! ATTACCAAGGTCACTTTTCT+CGG + chr6.3:6582895-6582914 None:intergenic 35.0%
!!! TTTTTTCTTCTTCGTTTGCG+TGG - chr6.3:6582293-6582312 MS.gene20440:intron 35.0%
ACAGCCTGAGAAAATGGAAA+GGG + chr6.3:6583561-6583580 None:intergenic 40.0%
AGAAGATCAAGTGCTCATCA+AGG - chr6.3:6581822-6581841 MS.gene20440:CDS 40.0%
AGAGCCCTCTTAATATCAAC+AGG + chr6.3:6581868-6581887 None:intergenic 40.0%
AGATGCCTCAAAAACATGCT+TGG + chr6.3:6583112-6583131 None:intergenic 40.0%
ATGAGCACTTGATCTTCTCT+AGG + chr6.3:6581820-6581839 None:intergenic 40.0%
CCATCTGAACTGAGTAAGAA+AGG + chr6.3:6583468-6583487 None:intergenic 40.0%
CCTTTCTTACTCAGTTCAGA+TGG - chr6.3:6583465-6583484 MS.gene20440:CDS 40.0%
GAAAATGGAAAGGGACTCTT+AGG + chr6.3:6583552-6583571 None:intergenic 40.0%
GATGGTGATTGCAATCTTAC+TGG + chr6.3:6581922-6581941 None:intergenic 40.0%
GATTGCAATCTTACTGGCAA+AGG + chr6.3:6581916-6581935 None:intergenic 40.0%
GCTTGGGTTTACTCTCAATA+TGG - chr6.3:6581745-6581764 MS.gene20440:CDS 40.0%
GTCAAACCTACAGATTACCA+AGG + chr6.3:6582908-6582927 None:intergenic 40.0%
GTCGGACTTTACTTATCTTC+TGG + chr6.3:6582930-6582949 None:intergenic 40.0%
GTTGATATTAAGAGGGCTCT+TGG - chr6.3:6581868-6581887 MS.gene20440:CDS 40.0%
GTTGTGACAATATTACCACC+TGG - chr6.3:6583426-6583445 MS.gene20440:intron 40.0%
TGAGCACTTGATCTTCTCTA+GGG + chr6.3:6581819-6581838 None:intergenic 40.0%
! AAGTGACCTTGGTAATCTGT+AGG - chr6.3:6582899-6582918 MS.gene20440:CDS 40.0%
! ACCCAAGCTTTTGTATGAGA+TGG + chr6.3:6581733-6581752 None:intergenic 40.0%
! CACAACTTTTCCTCCTTCAT+TGG + chr6.3:6583412-6583431 None:intergenic 40.0%
! GATTGGTCGTAGTTTCTTGA+CGG - chr6.3:6582210-6582229 MS.gene20440:CDS 40.0%
! GTTCTCTTTTCTTGGGAACA+AGG + chr6.3:6582725-6582744 None:intergenic 40.0%
! TGGAGAATCTGTTTGGTTTC+TGG + chr6.3:6582968-6582987 None:intergenic 40.0%
!! GAATCTGTTTGGTTTCTGGT+TGG + chr6.3:6582964-6582983 None:intergenic 40.0%
!!! TTACCAAGGTCACTTTTCTC+GGG + chr6.3:6582894-6582913 None:intergenic 40.0%
ACACCAGCTACATCATATCC+TGG + chr6.3:6582335-6582354 None:intergenic 45.0%
ACCGGCAATCTAATGCAATC+AGG - chr6.3:6582133-6582152 MS.gene20440:CDS 45.0%
AGGATATGATGTAGCTGGTG+TGG - chr6.3:6582334-6582353 MS.gene20440:intron 45.0%
AGTAAGAAAGGGATAGCTGG+TGG + chr6.3:6583456-6583475 None:intergenic 45.0%
ATATGATGTAGCTGGTGTGG+TGG - chr6.3:6582337-6582356 MS.gene20440:intron 45.0%
CACAGCCTGAGAAAATGGAA+AGG + chr6.3:6583562-6583581 None:intergenic 45.0%
CTGAGTAAGAAAGGGATAGC+TGG + chr6.3:6583459-6583478 None:intergenic 45.0%
GATGTGGTGTATGATGCAGT+AGG - chr6.3:6583243-6583262 MS.gene20440:intron 45.0%
GCCTGATTGCATTAGATTGC+CGG + chr6.3:6582137-6582156 None:intergenic 45.0%
GGACTCTTAGGGTCCAATAT+TGG + chr6.3:6583540-6583559 None:intergenic 45.0%
GTTCCCAAGAAAAGAGAACC+TGG - chr6.3:6582726-6582745 MS.gene20440:CDS 45.0%
TACGGTACACTCGCAAATTG+AGG + chr6.3:6581975-6581994 None:intergenic 45.0%
TTTCCCGAGAAAAGTGACCT+TGG - chr6.3:6582888-6582907 MS.gene20440:CDS 45.0%
TTTCTTCTTCGTTTGCGTGG+TGG - chr6.3:6582296-6582315 MS.gene20440:intron 45.0%
! CTTTCCATTTTCTCAGGCTG+TGG - chr6.3:6583560-6583579 MS.gene20440:CDS 45.0%
! GAGTCCCTTTCCATTTTCTC+AGG - chr6.3:6583554-6583573 MS.gene20440:CDS 45.0%
!! ACTTGATCTTCTCTAGGGTG+TGG + chr6.3:6581814-6581833 None:intergenic 45.0%
!! GCTAGCCAAGCATGTTTTTG+AGG - chr6.3:6583104-6583123 MS.gene20440:intron 45.0%
!! TGAAATAAAAAATATTAAAT+AGG + chr6.3:6583355-6583374 None:intergenic 5.0%
!!! ATATTTTTTATTTCAAAAAT+AGG - chr6.3:6583360-6583379 MS.gene20440:intron 5.0%
CTACTTGTTCTTGCAGGTGC+TGG - chr6.3:6582602-6582621 MS.gene20440:intron 50.0%
GAGGAAATTGGGAGCAGACT+TGG - chr6.3:6583173-6583192 MS.gene20440:intron 50.0%
GAGGGCTCTTGGTCATTTCA+AGG - chr6.3:6581879-6581898 MS.gene20440:CDS 50.0%
GCTTCCACAGCCTGAGAAAA+TGG + chr6.3:6583567-6583586 None:intergenic 50.0%
GTGCCAGGATATGATGTAGC+TGG - chr6.3:6582329-6582348 MS.gene20440:intron 50.0%
! TGATAGTGAGAGGGCAGTGA+AGG - chr6.3:6583383-6583402 MS.gene20440:intron 50.0%
!! CTTCTCTAGGGTGTGGTGTA+CGG + chr6.3:6581807-6581826 None:intergenic 50.0%
ATAGCTGGTGGAGTTCCAGG+TGG + chr6.3:6583444-6583463 None:intergenic 55.0%
GACGGCGTATAACACCGACA+AGG - chr6.3:6582228-6582247 MS.gene20440:CDS 55.0%
GCAGTGAAGGCTGCCAATGA+AGG - chr6.3:6583396-6583415 MS.gene20440:intron 55.0%
GCTGGTGTGGTGGTTAGTGT+TGG - chr6.3:6582347-6582366 MS.gene20440:intron 55.0%
GGGTGTGGTGTACGGACATT+AGG + chr6.3:6581799-6581818 None:intergenic 55.0%
GTGAAGGCTGCCAATGAAGG+AGG - chr6.3:6583399-6583418 MS.gene20440:intron 55.0%
!! CTTGTTCTTGCAGGTGCTGG+AGG - chr6.3:6582605-6582624 MS.gene20440:intron 55.0%
GGGATAGCTGGTGGAGTTCC+AGG + chr6.3:6583447-6583466 None:intergenic 60.0%
GGTGGTGTGAAGACTGTGCC+AGG - chr6.3:6582314-6582333 MS.gene20440:intron 60.0%
!! CTTGCAGGTGCTGGAGGAGT+TGG - chr6.3:6582611-6582630 MS.gene20440:intron 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 6581709 6583647 6581709 ID=MS.gene20440
chr6.3 mRNA 6581709 6583647 6581709 ID=MS.gene20440.t1;Parent=MS.gene20440
chr6.3 exon 6583435 6583647 6583435 ID=MS.gene20440.t1.exon1;Parent=MS.gene20440.t1
chr6.3 CDS 6583435 6583647 6583435 ID=cds.MS.gene20440.t1;Parent=MS.gene20440.t1
chr6.3 exon 6582707 6583030 6582707 ID=MS.gene20440.t1.exon2;Parent=MS.gene20440.t1
chr6.3 CDS 6582707 6583030 6582707 ID=cds.MS.gene20440.t1;Parent=MS.gene20440.t1
chr6.3 exon 6582092 6582251 6582092 ID=MS.gene20440.t1.exon3;Parent=MS.gene20440.t1
chr6.3 CDS 6582092 6582251 6582092 ID=cds.MS.gene20440.t1;Parent=MS.gene20440.t1
chr6.3 exon 6581709 6581974 6581709 ID=MS.gene20440.t1.exon4;Parent=MS.gene20440.t1
chr6.3 CDS 6581709 6581974 6581709 ID=cds.MS.gene20440.t1;Parent=MS.gene20440.t1
Gene Sequence

>MS.gene20440

ATGGCAAGCACACATAGTATTCCATCTCATACAAAAGCTTGGGTTTACTCTCAATATGGTAACATAGAAGAAATTCTAAAGTTTGATCCTAATGTCCGTACACCACACCCTAGAGAAGATCAAGTGCTCATCAAGGTTGTAGCTGCTGCCCTTAATCCTGTTGATATTAAGAGGGCTCTTGGTCATTTCAAGGACATTGACTCTCCTTTGCCAGTAAGATTGCAATCACCATCTATGACTTAAATTTCAAGAGTATCAGTACACCTCAATTTGCGAGTGTACCGTAGAAGTTTTCATTTATATTTTTAGTCCTGTAAAAAAAATTCTTTGTTTATAGCCGTTGCAAACATATAATGTTTTTGCTTTTGATTCTATTTGTTATCTTAATTCATATGCACCGTTAGTGTAATTAAATAATTTTTACACCGGCAATCTAATGCAATCAGGCAGATCATTAAAAGCGTCTACTTAGATCTTAATTATTTCTAAAATCACACTTATGATTGGTCGTAGTTTCTTGACGGCGTATAACACCGACAAGGTACCAAAATGAAACTCACAAACATAATGCTAAATAACATGTTTTTTTTCTTCTTCGTTTGCGTGGTGGTGTGAAGACTGTGCCAGGATATGATGTAGCTGGTGTGGTGGTTAGTGTTGGAGACCAAGTGAAGAAATTTAAGGTTGGAGATGAAGTTTATGGTGATATCAATGAGATTACGTTACACAATCCAAAAACTATTGGTACTTTGTCAGAGTATACTGTTGCTGAAGAAAAAGTACTTGCTCACAAACCATCAAATTTGAGTTTTGTTGAAGCTGCTAGTCTCCCTTTAGCAATAATTACTGCTTATCAAGGACTTGAAAGAGTTGAATTTTCTTCTGGTAAATCTCTACTTGTTCTTGCAGGTGCTGGAGGAGTTGGATCTCTTGTTATTCAGGTTTGAATTCTTCTTTCTAAAGATACTCTATACTAGGAATAAATATGATTTTTTTCTTTTTTTGTCTTAATCCCTTGTTCCCAAGAAAAGAGAACCTGGTTAAAGTCGGACTAAAAATTGTTCTAATCAGAAATATAACAGGTTTTCACAAACAATTTGTCCTTAACTAAAGATTGACCAGAAGATAAGTAGTAAATCCGTAATAAATAAGATGAACATTTTTTGTTTTAATTCTGTGTTTCCCGAGAAAAGTGACCTTGGTAATCTGTAGGTTTGACCAGAAGATAAGTAAAGTCCGACTAAAGATTGTTCCAACCAGAAACCAAACAGATTCTCCAAACAATTCGTCCTTAACTTATTCATTGCTCAATATAAGTTGGCTTTTGTAATACATAAAGTATAGAATGTAGTATTTTGTCATCTAATGGAATGCAATTATTTGTTTTGGACTATAGCTAGCCAAGCATGTTTTTGAGGCATCTAGAATTGCAGCTACTGCTAGCACAACAAAACTTGATTTTTTGAGGAAATTGGGAGCAGACTTGGCAATTGATTATACAAAGGAGAATTATGAAGAACTAACTGAAAAGTTTGATGTGGTGTATGATGCAGTAGGTAAGATATTTCTTCTAAATTTAAATCCTTATTAATATGTTGTATTATGTGTAAATTTTCATTGCAAGAAAACAATATAGTTAAAAACCTATTTAATATTTTTTATTTCAAAAATAGGTGATAGTGAGAGGGCAGTGAAGGCTGCCAATGAAGGAGGAAAAGTTGTGACAATATTACCACCTGGAACTCCACCAGCTATCCCTTTCTTACTCAGTTCAGATGGAGCTGTGTTAGAGAAATTGAAACCTTACTTGGAAAATGGCAAAGTGAAACCAATATTGGACCCTAAGAGTCCCTTTCCATTTTCTCAGGCTGTGGAAGCATTTACCTATCTTAATACTAATAGAGCTATTGGGAAAATAGTTATTCACCCCATTCCATGA
Protein sequence

>MS.gene20440.t1

MASTHSIPSHTKAWVYSQYGNIEEILKFDPNVRTPHPREDQVLIKVVAAALNPVDIKRALGHFKDIDSPLPTVPGYDVAGVVVSVGDQVKKFKVGDEVYGDINEITLHNPKTIGTLSEYTVAEEKVLAHKPSNLSFVEAASLPLAIITAYQGLERVEFSSGKSLLVLAGAGGVGSLVIQLAKHVFEASRIAATASTTKLDFLRKLGADLAIDYTKENYEELTEKFDVVYDAVGDSERAVKAANEGGKVVTILPPGTPPAIPFLLSSDGAVLEKLKPYLENGKVKPILDPKSPFPFSQAVEAFTYLNTNRAIGKIVIHPIP