AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20563

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTCAGAGATTTCTCTTTC+TGG 0.113684 6.3:+4916944 MS.gene20563:CDS
CTGAATTCTGTCCTTATTCT+TGG 0.185266 6.3:-4918958 None:intergenic
TGCTATTTGTCGCTCTCTTC+AGG 0.192172 6.3:+4919175 MS.gene20563:CDS
GCTATATTTGTCCAAGAATA+AGG 0.275459 6.3:+4918947 MS.gene20563:CDS
ACAATGGCGGATTCTGGTTC+TGG 0.335048 6.3:+4916676 None:intergenic
GGATCTGAGATTTCACTTGC+AGG 0.367818 6.3:+4916793 MS.gene20563:CDS
AGGACAGAATTCAGTTAGAT+AGG 0.402272 6.3:+4918967 MS.gene20563:CDS
GCTATTTGTCGCTCTCTTCA+GGG 0.407278 6.3:+4919176 MS.gene20563:CDS
AGTTGGACTTTAAGAAGGCT+TGG 0.415881 6.3:+4919096 MS.gene20563:CDS
AGAAGTTGATGCGGCTAAGT+TGG 0.447673 6.3:+4919079 MS.gene20563:CDS
GATTTCATCATCGATGACTT+AGG 0.470876 6.3:+4916908 MS.gene20563:intron
TGGGACAAGGTGATGTGTGA+GGG 0.474973 6.3:+4919209 MS.gene20563:CDS
GTGTGAGGGTTGTCGCTCAT+TGG 0.476725 6.3:+4919223 MS.gene20563:CDS
GGCTAAGTTGGACTTTAAGA+AGG 0.492218 6.3:+4919091 MS.gene20563:CDS
CTTCACATACAAGCACTTGT+CGG 0.515538 6.3:-4919246 None:intergenic
CAAAATGGAAGTTCGCACTA+AGG 0.521021 6.3:+4918878 MS.gene20563:CDS
ATAATCTTTCGGCTGAGGAA+AGG 0.544375 6.3:+4919129 MS.gene20563:CDS
GCATCATCATCCGACTCACC+AGG 0.546275 6.3:-4919047 None:intergenic
AGTTCGCACTAAGGATAAGA+AGG 0.554947 6.3:+4918887 MS.gene20563:CDS
GTGGGACAAGGTGATGTGTG+AGG 0.556896 6.3:+4919208 MS.gene20563:CDS
CTTTCGGCTGAGGAAAGGAC+TGG 0.572748 6.3:+4919134 MS.gene20563:CDS
GGCTGAGGAAAGGACTGGAC+TGG 0.577675 6.3:+4919139 MS.gene20563:CDS
GTGCTTGTATGTGAAGATAG+TGG 0.618718 6.3:+4919253 MS.gene20563:CDS
CAGCTCAACAGAAAACATCA+AGG 0.627779 6.3:-4919276 None:intergenic
TGAGGAAAGGACTGGACTGG+TGG 0.649015 6.3:+4919142 MS.gene20563:CDS
TAAAGAGTCAGAAGTTGATG+CGG 0.687717 6.3:+4919070 MS.gene20563:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGATTGAATATTTTAAT+TGG + chr6.3:4917628-4917647 MS.gene20563:intron 10.0%
!!! TATTTTTTTTGTTTTTTGAA+GGG - chr6.3:4917834-4917853 None:intergenic 10.0%
!! AAAATTTACAAATCAGATTT+CGG - chr6.3:4918823-4918842 None:intergenic 15.0%
!! GTAAAATTTAAATTTCTTGA+TGG + chr6.3:4917470-4917489 MS.gene20563:intron 15.0%
!! TATATAACAGTTGTTAAAAA+AGG - chr6.3:4917373-4917392 None:intergenic 15.0%
!!! ACATGTGAATTTTTAATTTA+AGG + chr6.3:4918041-4918060 MS.gene20563:intron 15.0%
!!! GATTATAGATTAAAAGATTT+TGG + chr6.3:4918710-4918729 MS.gene20563:intron 15.0%
!!! GTATTTTTTTTGTTTTTTGA+AGG - chr6.3:4917835-4917854 None:intergenic 15.0%
!!! TTAGATGATAGATTAAATTT+TGG + chr6.3:4917325-4917344 MS.gene20563:intron 15.0%
!! AAAATACTTATGTATAGTCT+TGG + chr6.3:4918407-4918426 MS.gene20563:intron 20.0%
!! ATATAAAATGTACACTAAAG+TGG + chr6.3:4917883-4917902 MS.gene20563:intron 20.0%
!! ATATTAAGTTTCTGAACATT+AGG + chr6.3:4917216-4917235 MS.gene20563:intron 20.0%
!! TGTGTAGTATAATAATAAGA+GGG - chr6.3:4918221-4918240 None:intergenic 20.0%
!! TTAGCAAAACTATCTTTATT+CGG - chr6.3:4917180-4917199 None:intergenic 20.0%
!! TTCAAATGACAATTTCAATA+TGG + chr6.3:4917920-4917939 MS.gene20563:intron 20.0%
!! TTGTGTAGTATAATAATAAG+AGG - chr6.3:4918222-4918241 None:intergenic 20.0%
!!! GAAAAATAGCATTATTTTAG+AGG - chr6.3:4918183-4918202 None:intergenic 20.0%
!!! GAATGAGTTTTATAATCTTT+CGG + chr6.3:4919118-4919137 MS.gene20563:CDS 20.0%
!!! TACTTTTTATGCATATGTAT+GGG + chr6.3:4918104-4918123 MS.gene20563:intron 20.0%
!!! TATAATCTAAGATGTAAAGA+GGG - chr6.3:4918697-4918716 None:intergenic 20.0%
!!! TTATTTTTGATGTTGAATTG+CGG + chr6.3:4917268-4917287 MS.gene20563:intron 20.0%
!!! TTCTTTTTTTGAAGGATAAT+TGG + chr6.3:4917656-4917675 MS.gene20563:intron 20.0%
! AAATTATGCTTATTGTGGTA+AGG + chr6.3:4917778-4917797 MS.gene20563:intron 25.0%
! AACAGTTGTTAAAAAAGGTA+TGG - chr6.3:4917368-4917387 None:intergenic 25.0%
! AATACATGATCAATTAAGCT+AGG + chr6.3:4917245-4917264 MS.gene20563:intron 25.0%
! ATGACAATTTCAATATGGTT+AGG + chr6.3:4917925-4917944 MS.gene20563:intron 25.0%
! CAAGAAAATTATGCTTATTG+TGG + chr6.3:4917773-4917792 MS.gene20563:intron 25.0%
! CAATAAAAGTTCAGCAAAAT+TGG + chr6.3:4918430-4918449 MS.gene20563:intron 25.0%
! CAGGTATATCATCTATTATT+TGG - chr6.3:4917573-4917592 None:intergenic 25.0%
! TAGCAAAACTATCTTTATTC+GGG - chr6.3:4917179-4917198 None:intergenic 25.0%
! TCAAACTAAAATGCAATATC+AGG - chr6.3:4917592-4917611 None:intergenic 25.0%
!! ACTTTTTATGCATATGTATG+GGG + chr6.3:4918105-4918124 MS.gene20563:intron 25.0%
!! ATTCATTATTGCTGAGTTTT+TGG + chr6.3:4918567-4918586 MS.gene20563:intron 25.0%
!! CTATAATCTAAGATGTAAAG+AGG - chr6.3:4918698-4918717 None:intergenic 25.0%
!! TTCATTATTGCTGAGTTTTT+GGG + chr6.3:4918568-4918587 MS.gene20563:intron 25.0%
!!! AAGATAGTTTTGCTAACATT+AGG + chr6.3:4917184-4917203 MS.gene20563:intron 25.0%
!!! CTACTTTTTATGCATATGTA+TGG + chr6.3:4918103-4918122 MS.gene20563:intron 25.0%
!!! TGGATTACTTCTTTTTTTGA+AGG + chr6.3:4917648-4917667 MS.gene20563:intron 25.0%
AATTTCAATATGGTTAGGTG+TGG + chr6.3:4917930-4917949 MS.gene20563:intron 30.0%
ACAACAGTTATTGAAAGGTA+TGG - chr6.3:4918753-4918772 None:intergenic 30.0%
ACACAGTATCTTATAGTTGT+AGG + chr6.3:4918295-4918314 MS.gene20563:intron 30.0%
ATTCAAAGTGTATCAGAAAG+CGG - chr6.3:4917048-4917067 None:intergenic 30.0%
GCTATATTTGTCCAAGAATA+AGG + chr6.3:4918947-4918966 MS.gene20563:CDS 30.0%
GGATTCTCAAAAAACTTATC+TGG - chr6.3:4919029-4919048 None:intergenic 30.0%
GTAGTATAATAATAAGAGGG+TGG - chr6.3:4918218-4918237 None:intergenic 30.0%
! CATTTTGAATGAGCTCAAAA+TGG + chr6.3:4918863-4918882 MS.gene20563:CDS 30.0%
! CATTTTGAGCTCATTCAAAA+TGG - chr6.3:4918865-4918884 None:intergenic 30.0%
! GATTTTAGACAAGCTTTACA+GGG + chr6.3:4918651-4918670 MS.gene20563:intron 30.0%
! TGATTTTAGACAAGCTTTAC+AGG + chr6.3:4918650-4918669 MS.gene20563:intron 30.0%
!!! ATGCTATTTTTCCAAATGAG+TGG + chr6.3:4918191-4918210 MS.gene20563:intron 30.0%
!!! GATAAGTTTTTTGAGAATCC+TGG + chr6.3:4919029-4919048 MS.gene20563:CDS 30.0%
AGGACAGAATTCAGTTAGAT+AGG + chr6.3:4918967-4918986 MS.gene20563:CDS 35.0%
ATCAAGTTCAAATAAGCACC+AGG + chr6.3:4918504-4918523 MS.gene20563:intron 35.0%
CGTGTACAACAGTTATTGAA+AGG - chr6.3:4918758-4918777 None:intergenic 35.0%
CTCAAATTATGTGGTGTAAC+TGG + chr6.3:4917981-4918000 MS.gene20563:intron 35.0%
CTGAATTCTGTCCTTATTCT+TGG - chr6.3:4918961-4918980 None:intergenic 35.0%
GATTTCATCATCGATGACTT+AGG + chr6.3:4916908-4916927 MS.gene20563:intron 35.0%
TAGTCAATAAGCCCTGATAT+AGG + chr6.3:4918534-4918553 MS.gene20563:intron 35.0%
! CATTTTGTATGAGCTCAAGA+AGG + chr6.3:4917499-4917518 MS.gene20563:intron 35.0%
! GTTTTATAATCTTTCGGCTG+AGG + chr6.3:4919124-4919143 MS.gene20563:CDS 35.0%
! TGATTTTCAGATTCTGTTGC+AGG + chr6.3:4916772-4916791 MS.gene20563:intron 35.0%
! TTGATTATTCCCGATTTTGC+TGG + chr6.3:4917071-4917090 MS.gene20563:intron 35.0%
!! TAAAGAGTCAGAAGTTGATG+CGG + chr6.3:4919070-4919089 MS.gene20563:CDS 35.0%
ACACATCAATCGAGAGCTTA+AGG - chr6.3:4917549-4917568 None:intergenic 40.0%
ACCTGGGATCTCAAATTATG+TGG + chr6.3:4917972-4917991 MS.gene20563:intron 40.0%
AGTTCGCACTAAGGATAAGA+AGG + chr6.3:4918887-4918906 MS.gene20563:CDS 40.0%
ATAATCTTTCGGCTGAGGAA+AGG + chr6.3:4919129-4919148 MS.gene20563:CDS 40.0%
ATCCAACATGAGCCTATATC+AGG - chr6.3:4918549-4918568 None:intergenic 40.0%
ATCTAAGTAAACTCCTCCCT+CGG - chr6.3:4917311-4917330 None:intergenic 40.0%
ATGTTGAATTGCGGAGTTCT+TGG + chr6.3:4917277-4917296 MS.gene20563:intron 40.0%
CAAAATGGAAGTTCGCACTA+AGG + chr6.3:4918878-4918897 MS.gene20563:CDS 40.0%
CACTACACACATAACTCTCT+AGG + chr6.3:4918370-4918389 MS.gene20563:intron 40.0%
CAGCTCAACAGAAAACATCA+AGG - chr6.3:4919279-4919298 None:intergenic 40.0%
CGTTCAGAGATTTCTCTTTC+TGG + chr6.3:4916944-4916963 MS.gene20563:CDS 40.0%
CTTCACATACAAGCACTTGT+CGG - chr6.3:4919249-4919268 None:intergenic 40.0%
GTACACTAAAGTGGTGTGTA+AGG + chr6.3:4917892-4917911 MS.gene20563:intron 40.0%
GTGCTTGTATGTGAAGATAG+TGG + chr6.3:4919253-4919272 MS.gene20563:CDS 40.0%
TATTCCACCACCAGCAAAAT+CGG - chr6.3:4917084-4917103 None:intergenic 40.0%
TCCAACATGAGCCTATATCA+GGG - chr6.3:4918548-4918567 None:intergenic 40.0%
TGTTGAATTGCGGAGTTCTT+GGG + chr6.3:4917278-4917297 MS.gene20563:intron 40.0%
TTAGACAAGCTTTACAGGGT+TGG + chr6.3:4918655-4918674 MS.gene20563:intron 40.0%
! ACCACATAATTTGAGATCCC+AGG - chr6.3:4917976-4917995 None:intergenic 40.0%
! AGTTGGACTTTAAGAAGGCT+TGG + chr6.3:4919096-4919115 MS.gene20563:CDS 40.0%
! ATTATTCCCGATTTTGCTGG+TGG + chr6.3:4917074-4917093 MS.gene20563:intron 40.0%
! CTTATTGACTAAGTGAAGCC+TGG - chr6.3:4918525-4918544 None:intergenic 40.0%
! CTTGATGTTTTCTGTTGAGC+TGG + chr6.3:4919277-4919296 MS.gene20563:CDS 40.0%
! GGCTAAGTTGGACTTTAAGA+AGG + chr6.3:4919091-4919110 MS.gene20563:CDS 40.0%
!! TGCAGGTTAGTCTTAACTCT+AGG + chr6.3:4916810-4916829 MS.gene20563:intron 40.0%
!! TTTTGAGAATCCTGGTGAGT+CGG + chr6.3:4919037-4919056 MS.gene20563:CDS 40.0%
!!! AGTTTTTGGGATTTGAACCG+AGG + chr6.3:4918581-4918600 MS.gene20563:intron 40.0%
!!! GTTTTTGGGATTTGAACCGA+GGG + chr6.3:4918582-4918601 MS.gene20563:intron 40.0%
AGTTCTTGGGATTTGAACCG+AGG + chr6.3:4917291-4917310 MS.gene20563:intron 45.0%
ATTCCACCACCAGCAAAATC+GGG - chr6.3:4917083-4917102 None:intergenic 45.0%
GCTATTTGTCGCTCTCTTCA+GGG + chr6.3:4919176-4919195 MS.gene20563:CDS 45.0%
GTTCTTGGGATTTGAACCGA+GGG + chr6.3:4917292-4917311 MS.gene20563:intron 45.0%
TAATCTCTACTCTCCTCCCT+CGG - chr6.3:4918601-4918620 None:intergenic 45.0%
TTGAGATCCCAGGTTTGATC+CGG - chr6.3:4917966-4917985 None:intergenic 45.0%
! GATGTTTTCTGTTGAGCTGG+AGG + chr6.3:4919280-4919299 MS.gene20563:CDS 45.0%
! GGATCTGAGATTTCACTTGC+AGG + chr6.3:4916793-4916812 MS.gene20563:CDS 45.0%
! TCGCTCTCTTCAGGGTTTTA+AGG + chr6.3:4919184-4919203 MS.gene20563:CDS 45.0%
! TGCTATTTGTCGCTCTCTTC+AGG + chr6.3:4919175-4919194 MS.gene20563:CDS 45.0%
! TTTTCACCCGGATCAAACCT+GGG + chr6.3:4917956-4917975 MS.gene20563:intron 45.0%
!! AGAAGTTGATGCGGCTAAGT+TGG + chr6.3:4919079-4919098 MS.gene20563:CDS 45.0%
!! CGCTCTCTTCAGGGTTTTAA+GGG + chr6.3:4919185-4919204 MS.gene20563:CDS 45.0%
!!! GCTCTCTTCAGGGTTTTAAG+GGG + chr6.3:4919186-4919205 MS.gene20563:CDS 45.0%
!!! TCTTCAGGGTTTTAAGGGGT+GGG + chr6.3:4919190-4919209 MS.gene20563:CDS 45.0%
AAGAGGGTGGTCCACTCATT+TGG - chr6.3:4918205-4918224 None:intergenic 50.0%
CAGGTTTGATCCGGGTGAAA+AGG - chr6.3:4917957-4917976 None:intergenic 50.0%
GCCCTGATATAGGCTCATGT+TGG + chr6.3:4918544-4918563 MS.gene20563:intron 50.0%
TGAGATCCCAGGTTTGATCC+GGG - chr6.3:4917965-4917984 None:intergenic 50.0%
TGGGACAAGGTGATGTGTGA+GGG + chr6.3:4919209-4919228 MS.gene20563:CDS 50.0%
! ATTCCCGATTTTGCTGGTGG+TGG + chr6.3:4917077-4917096 MS.gene20563:intron 50.0%
! CTTTTCACCCGGATCAAACC+TGG + chr6.3:4917955-4917974 MS.gene20563:intron 50.0%
! TAGGTGTGGACCTTTTCACC+CGG + chr6.3:4917944-4917963 MS.gene20563:intron 50.0%
! TTTGGGATTTGAACCGAGGG+AGG + chr6.3:4918585-4918604 MS.gene20563:intron 50.0%
!!! CTCTTCAGGGTTTTAAGGGG+TGG + chr6.3:4919189-4919208 MS.gene20563:CDS 50.0%
CTTGGGATTTGAACCGAGGG+AGG + chr6.3:4917295-4917314 MS.gene20563:intron 55.0%
CTTTCGGCTGAGGAAAGGAC+TGG + chr6.3:4919134-4919153 MS.gene20563:CDS 55.0%
GCATCATCATCCGACTCACC+AGG - chr6.3:4919050-4919069 None:intergenic 55.0%
GTGGGACAAGGTGATGTGTG+AGG + chr6.3:4919208-4919227 MS.gene20563:CDS 55.0%
GTGTGAGGGTTGTCGCTCAT+TGG + chr6.3:4919223-4919242 MS.gene20563:CDS 55.0%
! TGAGGAAAGGACTGGACTGG+TGG + chr6.3:4919142-4919161 MS.gene20563:CDS 55.0%
!! TGGCGGATTCTGGTTCTGGT+CGG + chr6.3:4916680-4916699 MS.gene20563:intron 55.0%
!!! GGGTTTTAAGGGGTGGGACA+AGG + chr6.3:4919196-4919215 MS.gene20563:CDS 55.0%
GGCTGAGGAAAGGACTGGAC+TGG + chr6.3:4919139-4919158 MS.gene20563:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 4916679 4919307 4916679 ID=MS.gene20563
chr6.3 mRNA 4916679 4919307 4916679 ID=MS.gene20563.t1;Parent=MS.gene20563
chr6.3 exon 4916679 4916697 4916679 ID=MS.gene20563.t1.exon1;Parent=MS.gene20563.t1
chr6.3 CDS 4916679 4916697 4916679 ID=cds.MS.gene20563.t1;Parent=MS.gene20563.t1
chr6.3 exon 4916782 4916814 4916782 ID=MS.gene20563.t1.exon2;Parent=MS.gene20563.t1
chr6.3 CDS 4916782 4916814 4916782 ID=cds.MS.gene20563.t1;Parent=MS.gene20563.t1
chr6.3 exon 4916909 4916965 4916909 ID=MS.gene20563.t1.exon3;Parent=MS.gene20563.t1
chr6.3 CDS 4916909 4916965 4916909 ID=cds.MS.gene20563.t1;Parent=MS.gene20563.t1
chr6.3 exon 4918862 4919307 4918862 ID=MS.gene20563.t1.exon4;Parent=MS.gene20563.t1
chr6.3 CDS 4918862 4919307 4918862 ID=cds.MS.gene20563.t1;Parent=MS.gene20563.t1
Gene Sequence

>MS.gene20563

ATGGCGGATTCTGGTTCTGGTCGGTTAATTTTGATCTATAATTAATCTTCGATTTCAATTTCGATTTTAATTATTAATTTTTTCGTTTTTGCATGATTTTCAGATTCTGTTGCAGGATCTGAGATTTCACTTGCAGGTTAGTCTTAACTCTAGGTTTCAATTTTATTACGAATTTTGTATTGTTCTTTGATCGCTCAATAATAATGTTCTCTTTTCATGATTTTGTTCAGATTTCATCATCGATGACTTAGGTGATCTCACTCTTCGTTCAGAGATTTCTCTTTCTGGTACGTTTTGAAATCTTTAACGCTCTTGCATTTCTTCAACAATTGAGCTACTTAATGCTAAAAAATGCATGCATAATTTCCGCTTTCTGATACACTTTGAATTTCTTGATTATTCCCGATTTTGCTGGTGGTGGAATAAATCTTGCATGATTTTGTGTTTCTTAGACAATTGAGCTATTAAATGATAAAAAATGCATGCATAATTGTGTTCCCGAATAAAGATAGTTTTGCTAACATTAGGAACTTGAGAATATTAAGTTTCTGAACATTAGGATAAATAATACATGATCAATTAAGCTAGGTTATTTTTGATGTTGAATTGCGGAGTTCTTGGGATTTGAACCGAGGGAGGAGTTTACTTAGATGATAGATTAAATTTTGGTGAATTTGCATAAAGTTCCATACCTTTTTTAACAACTGTTATATATACACGTTTTTTTTAATTTATTATTTAGTTTATTTTTTTGATGTTAAATCTGTTTTGTAATATCTAATATCTGATTTGTAAAATTTAAATTTCTTGATGGTTAAGTCATTTTGTATGAGCTCAAGAAGGAAAAAAGCTTTTCATGCTTAAATTCCTTAAGCTCTCGATTGATGTGTACCAAATAATAGATGATATACCTGATATTGCATTTTAGTTTGATTTTTATTTCAATTATAATTGATTGAATATTTTAATTGGATTACTTCTTTTTTTGAAGGATAATTGGATATTTTTCTATACTTCTATAAATAGAAATTATATTGTTGCATGTATTATTGTTAACATAGTATATCACATTAGTTATAACTTTTTGTTGATGACAAGAAAATTATGCTTATTGTGGTAAGGTAATGTTTTCTTCTTATTACATCAAAATTTCCCTTCAAAAAACAAAAAAAATACTGTCAAAATTTAGATGATCTTTTATCAAATATAAAATGTACACTAAAGTGGTGTGTAAGGTCTAGTTCAAATGACAATTTCAATATGGTTAGGTGTGGACCTTTTCACCCGGATCAAACCTGGGATCTCAAATTATGTGGTGTAACTGGTGTTTTAAATTTATTTGCTTTTAAAAAATTTAGTTATACATGTGAATTTTTAATTTAAGGTTAGTTTCAGTATTTTGACTTAATATGTGAATTTTCTTACTACTTTTTATGCATATGTATGGGGTATAGATAAATTATATAATGTAAAGATTGAAACTTGAAGAAATGTTATTCTGCCTCTAAAATAATGCTATTTTTCCAAATGAGTGGACCACCCTCTTATTATTATACTACACAATAGCATATAGTTGTAAGCACAAACATACTTACTCTGTTTTTTATATGCACAATACACAGTATCTTATAGTTGTAGGCACAAACATAGATACTCTGTTTTAGTATTTATACACTACATTGTTGTACGTACACTACACACATAACTCTCTAGGTAATTTACACTTCTAAAATACTTATGTATAGTCTTGGCAATAAAAGTTCAGCAAAATTGGTCTTCTCTGCAGTCTATTAACACTATTTCAATTCATTTTCTTTGCTAGTTTATCAAGTTCAAATAAGCACCAGGCTTCACTTAGTCAATAAGCCCTGATATAGGCTCATGTTGGATTCATTATTGCTGAGTTTTTGGGATTTGAACCGAGGGAGGAGAGTAGAGATTATTGTTAATGTTTGTTGAGTTTTTTAGATTTGATTTTAGACAAGCTTTACAGGGTTGGTGTAAGCTCTACACTTCCCTCTTTACATCTTAGATTATAGATTAAAAGATTTTGGTGAACTTGCATTAAGTTCCATACCTTTCAATAACTGTTGTACACGTTTTTAGTTTATTTTTTGTATGTTAAATCTGTTTTATAATATCCGAAATCTGATTTGTAAATTTTAAATTTCTTGATGTTTAAGCCATTTTGAATGAGCTCAAAATGGAAGTTCGCACTAAGGATAAGAAGGTTCTGTTTGATGAACTTCTTAATGAAGCTGCTGTCAAGCTATATTTGTCCAAGAATAAGGACAGAATTCAGTTAGATAGGAAAAATGAGTACTATGTCAAAGTGTTAGAGCAGAATCCAGATAAGTTTTTTGAGAATCCTGGTGAGTCGGATGATGATGCTAAAGAGTCAGAAGTTGATGCGGCTAAGTTGGACTTTAAGAAGGCTTGGAATGAGTTTTATAATCTTTCGGCTGAGGAAAGGACTGGACTGGTGGAGCAAGTTCGTGCTATTTGTCGCTCTCTTCAGGGTTTTAAGGGGTGGGACAAGGTGATGTGTGAGGGTTGTCGCTCATTGGCCGACAAGTGCTTGTATGTGAAGATAGTGGCCTTGATGTTTTCTGTTGAGCTGGAGGAGTAG
Protein sequence

>MS.gene20563.t1

MADSGSDSVAGSEISLADFIIDDLGDLTLRSEISLSAILNELKMEVRTKDKKVLFDELLNEAAVKLYLSKNKDRIQLDRKNEYYVKVLEQNPDKFFENPGESDDDAKESEVDAAKLDFKKAWNEFYNLSAEERTGLVEQVRAICRSLQGFKGWDKVMCEGCRSLADKCLYVKIVALMFSVELEE