AlfalfaGEDB | MS.gene20747

M. sativa cultivar XinJiangDaYe / MS.gene20747

KEGG: -
GO: -
PFAM: PF04398.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20747.t1	XP_003591500.1	93.8	162	8	1	1	160	1	162	1.10E-77	299.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20747.t1	Q9M015	30.5	95	65	1	26	120	56	149	7.7e-08	58.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20747.t1	G7IEM0	93.8	162	8	1	1	160	1	162	7.8e-78	299.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052224	MS.gene20747	0.810322	1.22E-50	-1.69E-46
MS.gene053401	MS.gene20747	-0.819764	9.78E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20747.t1	MTR_1g088250	93.827	162	8	1	1	160	1	162	2.27e-98	280
MS.gene20747.t1	MTR_1g088260	48.819	127	64	1	31	157	3	128	2.19e-44	142
MS.gene20747.t1	MTR_7g108775	47.222	144	73	2	6	147	11	153	1.69e-43	141
MS.gene20747.t1	MTR_7g108775	47.222	144	73	2	6	147	11	153	2.89e-43	142
MS.gene20747.t1	MTR_6g086680	36.364	143	80	4	5	136	7	149	8.00e-23	89.0
MS.gene20747.t1	MTR_8g085580	32.520	123	77	3	17	136	23	142	2.59e-15	69.7
MS.gene20747.t1	MTR_7g080280	28.226	124	84	2	23	143	40	161	5.05e-14	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20747.t1	AT1G02816	61.151	139	52	1	22	160	30	166	2.76e-60	184
MS.gene20747.t1	AT4G02370	53.503	157	68	2	8	160	12	167	9.12e-56	173
MS.gene20747.t1	AT4G02360	54.545	143	64	1	2	144	7	148	5.18e-54	168
MS.gene20747.t1	AT1G02813	45.139	144	76	2	3	144	2	144	3.72e-41	135
MS.gene20747.t1	AT5G19590	33.871	124	75	2	24	146	31	148	2.36e-17	74.7
MS.gene20747.t1	AT5G19590	33.871	124	75	2	24	146	31	148	2.36e-17	74.7
MS.gene20747.t1	AT5G16380	28.221	163	98	6	5	154	12	168	2.50e-14	67.8
MS.gene20747.t1	AT5G19860	34.234	111	67	3	23	130	35	142	1.46e-13	65.5
MS.gene20747.t1	AT3G07470	29.630	135	84	4	18	149	25	151	1.61e-13	64.7
MS.gene20747.t1	AT3G07470	28.571	147	92	5	8	149	13	151	1.64e-13	64.7
MS.gene20747.t1	AT3G07470	28.571	147	92	5	8	149	13	151	2.35e-13	64.3
MS.gene20747.t1	AT1G55265	34.711	121	71	4	25	141	55	171	1.57e-11	59.7
MS.gene20747.t1	AT5G01610	29.592	98	68	1	26	123	56	152	8.51e-11	57.8
MS.gene20747.t1	AT5G01610	29.592	98	68	1	26	123	56	152	8.51e-11	57.8

Find 50 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGTAGGCAGTTGGGTTTC+CGG	0.215272	1.2:-66097727	None:intergenic
GCGTCAAAGTGATGTTCTTC+TGG	0.264271	1.2:+66097952	MS.gene20747:CDS
AAAATAGACTTACCGATCTA+AGG	0.267447	1.2:+66097922	MS.gene20747:CDS
TGATTTGTCTAATTCGTAAC+TGG	0.313925	1.2:-66097799	None:intergenic
TGAATATTCTTGAAGTTGTT+AGG	0.334073	1.2:+66097976	MS.gene20747:CDS
TCCTCAGTGTGGTTGTGGTT+TGG	0.365789	1.2:+66098070	MS.gene20747:CDS
ACTCCTATCGGGAGAATTCC+CGG	0.413850	1.2:-66097774	None:intergenic
CGGTTAGCGCCGTCGCCGTC+AGG	0.414867	1.2:-66097701	None:intergenic
GAATCTCCTCAGTGTGGTTG+TGG	0.417985	1.2:+66098065	MS.gene20747:CDS
TCGTGTAGTTTCTCGTTACA+AGG	0.427526	1.2:+66097852	MS.gene20747:CDS
CGCAAGGCCTCGTAGGCAGT+TGG	0.436838	1.2:-66097735	None:intergenic
AGGAGGAGGAGGAGAACGAC+GGG	0.447449	1.2:-66097681	None:intergenic
GGCAGTTGGGTTTCCGGTGA+CGG	0.448621	1.2:-66097721	None:intergenic
CGTCAAAGTGATGTTCTTCT+GGG	0.456544	1.2:+66097953	MS.gene20747:CDS
CTCTGTTGGTGTTGCTTCTG+CGG	0.459033	1.2:+66098019	MS.gene20747:CDS
TTTAAGCAGAAGAAAGAGAA+GGG	0.461680	1.2:-66098133	None:intergenic
TCGTAACTGGTTACTCCTAT+CGG	0.463432	1.2:-66097786	None:intergenic
GCGCCGTCGCCGTCAGGAGG+AGG	0.477696	1.2:-66097695	None:intergenic
AAATAGACTTACCGATCTAA+GGG	0.481492	1.2:+66097923	MS.gene20747:CDS
GCAAGGCCTCGTAGGCAGTT+GGG	0.489260	1.2:-66097734	None:intergenic
CGTAACTGGTTACTCCTATC+GGG	0.502559	1.2:-66097785	None:intergenic
AACGATCACCGGTAAAATAA+CGG	0.509312	1.2:+66097899	MS.gene20747:CDS
CCGTCGCCGTCAGGAGGAGG+AGG	0.512636	1.2:-66097692	None:intergenic
GATGACCTTGATTTCTCTGT+TGG	0.513791	1.2:+66098005	MS.gene20747:CDS
AATTCCCGGTGGGAAATTGT+AGG	0.515251	1.2:-66097760	None:intergenic
CAACACCAACAGAGAAATCA+AGG	0.526235	1.2:-66098010	None:intergenic
GCGACGGCGCTAACCGTCAC+CGG	0.534133	1.2:+66097708	MS.gene20747:CDS
GACGCTTATACCCCTTAGAT+CGG	0.549482	1.2:-66097934	None:intergenic
CGTACCTACAATTTCCCACC+GGG	0.555651	1.2:+66097756	MS.gene20747:CDS
CCTCCTCCTCCTGACGGCGA+CGG	0.559285	1.2:+66097692	MS.gene20747:CDS
AGAACGACGGGGCTAGTCAT+CGG	0.573947	1.2:-66097669	None:intergenic
TTCTTTGAATCTCCTCAGTG+TGG	0.575291	1.2:+66098059	MS.gene20747:CDS
TTAGCGCCGTCGCCGTCAGG+AGG	0.577589	1.2:-66097698	None:intergenic
GCGTACCTACAATTTCCCAC+CGG	0.578143	1.2:+66097755	MS.gene20747:CDS
CAGGAGGAGGAGGAGAACGA+CGG	0.578931	1.2:-66097682	None:intergenic
CCACCGGGAATTCTCCCGAT+AGG	0.583587	1.2:+66097771	MS.gene20747:CDS
AGTTACAAATCAACGATCAC+CGG	0.584572	1.2:+66097888	MS.gene20747:CDS
TGGGAAATTGTAGGTACGCA+AGG	0.589209	1.2:-66097751	None:intergenic
CGTTCTCCTCCTCCTCCTGA+CGG	0.595497	1.2:+66097686	MS.gene20747:CDS
TACGAATTAGACAAATCAAG+TGG	0.598160	1.2:+66097804	MS.gene20747:CDS
CGGAAACCCAACTGCCTACG+AGG	0.623801	1.2:+66097728	MS.gene20747:CDS
GTAACGAGAAACTACACGAA+CGG	0.631098	1.2:-66097848	None:intergenic
GTAGGTACGCAAGGCCTCGT+AGG	0.643114	1.2:-66097742	None:intergenic
GATTCAAAGAAATTATCCAA+AGG	0.666602	1.2:-66098047	None:intergenic
CTATCGGGAGAATTCCCGGT+GGG	0.669781	1.2:-66097770	None:intergenic
AATAGACTTACCGATCTAAG+GGG	0.686558	1.2:+66097924	MS.gene20747:CDS
GGAGGAGGAGGAGAACGACG+GGG	0.724686	1.2:-66097680	None:intergenic
TCCAAACCACAACCACACTG+AGG	0.753988	1.2:-66098071	None:intergenic
CCTATCGGGAGAATTCCCGG+TGG	0.777816	1.2:-66097771	None:intergenic
ACGAACGGTTTAGATCAGCG+CGG	0.852399	1.2:-66097833	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GATTCAAAGAAATTATCCAA+AGG	-	chr1.2:66098050-66098069	None:intergenic	25.0%
!	TGAATATTCTTGAAGTTGTT+AGG	+	chr1.2:66097976-66097995	MS.gene20747:CDS	25.0%
!!!	TCTATTTTCCGTTATTTTAC+CGG	-	chr1.2:66097910-66097929	None:intergenic	25.0%
	AAAATAGACTTACCGATCTA+AGG	+	chr1.2:66097922-66097941	MS.gene20747:CDS	30.0%
	AAATAGACTTACCGATCTAA+GGG	+	chr1.2:66097923-66097942	MS.gene20747:CDS	30.0%
	TACGAATTAGACAAATCAAG+TGG	+	chr1.2:66097804-66097823	MS.gene20747:CDS	30.0%
!	TGATTTGTCTAATTCGTAAC+TGG	-	chr1.2:66097802-66097821	None:intergenic	30.0%
	AACGATCACCGGTAAAATAA+CGG	+	chr1.2:66097899-66097918	MS.gene20747:CDS	35.0%
	AATAGACTTACCGATCTAAG+GGG	+	chr1.2:66097924-66097943	MS.gene20747:CDS	35.0%
	AGTTACAAATCAACGATCAC+CGG	+	chr1.2:66097888-66097907	MS.gene20747:CDS	35.0%
	CAACACCAACAGAGAAATCA+AGG	-	chr1.2:66098013-66098032	None:intergenic	40.0%
	CGTCAAAGTGATGTTCTTCT+GGG	+	chr1.2:66097953-66097972	MS.gene20747:CDS	40.0%
	GATGACCTTGATTTCTCTGT+TGG	+	chr1.2:66098005-66098024	MS.gene20747:CDS	40.0%
	GTAACGAGAAACTACACGAA+CGG	-	chr1.2:66097851-66097870	None:intergenic	40.0%
	TCGTAACTGGTTACTCCTAT+CGG	-	chr1.2:66097789-66097808	None:intergenic	40.0%
	TCGTGTAGTTTCTCGTTACA+AGG	+	chr1.2:66097852-66097871	MS.gene20747:CDS	40.0%
	TTCTTTGAATCTCCTCAGTG+TGG	+	chr1.2:66098059-66098078	MS.gene20747:CDS	40.0%
	AATTCCCGGTGGGAAATTGT+AGG	-	chr1.2:66097763-66097782	None:intergenic	45.0%
	CGTAACTGGTTACTCCTATC+GGG	-	chr1.2:66097788-66097807	None:intergenic	45.0%
	GACGCTTATACCCCTTAGAT+CGG	-	chr1.2:66097937-66097956	None:intergenic	45.0%
	GCGTCAAAGTGATGTTCTTC+TGG	+	chr1.2:66097952-66097971	MS.gene20747:CDS	45.0%
	TGGGAAATTGTAGGTACGCA+AGG	-	chr1.2:66097754-66097773	None:intergenic	45.0%
	ACGAACGGTTTAGATCAGCG+CGG	-	chr1.2:66097836-66097855	None:intergenic	50.0%
	ACTCCTATCGGGAGAATTCC+CGG	-	chr1.2:66097777-66097796	None:intergenic	50.0%
	CGTACCTACAATTTCCCACC+GGG	+	chr1.2:66097756-66097775	MS.gene20747:CDS	50.0%
	GAATCTCCTCAGTGTGGTTG+TGG	+	chr1.2:66098065-66098084	MS.gene20747:CDS	50.0%
	GCGTACCTACAATTTCCCAC+CGG	+	chr1.2:66097755-66097774	MS.gene20747:CDS	50.0%
	TCCAAACCACAACCACACTG+AGG	-	chr1.2:66098074-66098093	None:intergenic	50.0%
	TCCTCAGTGTGGTTGTGGTT+TGG	+	chr1.2:66098070-66098089	MS.gene20747:CDS	50.0%
!	TGCTTCTGCGGCTTTTCCTT+TGG	+	chr1.2:66098031-66098050	MS.gene20747:CDS	50.0%
!!	CTCTGTTGGTGTTGCTTCTG+CGG	+	chr1.2:66098019-66098038	MS.gene20747:CDS	50.0%
	CTATCGGGAGAATTCCCGGT+GGG	-	chr1.2:66097773-66097792	None:intergenic	55.0%
	CTCGTAGGCAGTTGGGTTTC+CGG	-	chr1.2:66097730-66097749	None:intergenic	55.0%
	AGGAGGAGGAGGAGAACGAC+GGG	-	chr1.2:66097684-66097703	None:intergenic	60.0%
	CAGGAGGAGGAGGAGAACGA+CGG	-	chr1.2:66097685-66097704	None:intergenic	60.0%
	CCACCGGGAATTCTCCCGAT+AGG	+	chr1.2:66097771-66097790	MS.gene20747:CDS	60.0%
	CCTATCGGGAGAATTCCCGG+TGG	-	chr1.2:66097774-66097793	None:intergenic	60.0%
	CGGAAACCCAACTGCCTACG+AGG	+	chr1.2:66097728-66097747	MS.gene20747:CDS	60.0%
	CGTTCTCCTCCTCCTCCTGA+CGG	+	chr1.2:66097686-66097705	MS.gene20747:CDS	60.0%
	GCAAGGCCTCGTAGGCAGTT+GGG	-	chr1.2:66097737-66097756	None:intergenic	60.0%
	GGCAGTTGGGTTTCCGGTGA+CGG	-	chr1.2:66097724-66097743	None:intergenic	60.0%
	GTAGGTACGCAAGGCCTCGT+AGG	-	chr1.2:66097745-66097764	None:intergenic	60.0%
	CGCAAGGCCTCGTAGGCAGT+TGG	-	chr1.2:66097738-66097757	None:intergenic	65.0%
	GGAGGAGGAGGAGAACGACG+GGG	-	chr1.2:66097683-66097702	None:intergenic	65.0%
	CCTCCTCCTCCTGACGGCGA+CGG	+	chr1.2:66097692-66097711	MS.gene20747:CDS	70.0%
	GCGACGGCGCTAACCGTCAC+CGG	+	chr1.2:66097708-66097727	MS.gene20747:CDS	70.0%
	TTAGCGCCGTCGCCGTCAGG+AGG	-	chr1.2:66097701-66097720	None:intergenic	70.0%
	CCGTCGCCGTCAGGAGGAGG+AGG	-	chr1.2:66097695-66097714	None:intergenic	75.0%
	CGGTTAGCGCCGTCGCCGTC+AGG	-	chr1.2:66097704-66097723	None:intergenic	75.0%
!	GCGCCGTCGCCGTCAGGAGG+AGG	-	chr1.2:66097698-66097717	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	66097672	66098154	66097672	ID=MS.gene20747
chr1.2	mRNA	66097672	66098154	66097672	ID=MS.gene20747.t1;Parent=MS.gene20747
chr1.2	exon	66097672	66098154	66097672	ID=MS.gene20747.t1.exon1;Parent=MS.gene20747.t1
chr1.2	CDS	66097672	66098154	66097672	ID=cds.MS.gene20747.t1;Parent=MS.gene20747.t1

Gene Sequence

>MS.gene20747

ATGACTAGCCCCGTCGTTCTCCTCCTCCTCCTGACGGCGACGGCGCTAACCGTCACCGGAAACCCAACTGCCTACGAGGCCTTGCGTACCTACAATTTCCCACCGGGAATTCTCCCGATAGGAGTAACCAGTTACGAATTAGACAAATCAAGTGGCAATTTCCGCGCTGATCTAAACCGTTCGTGTAGTTTCTCGTTACAAGGTTCGTATCAATTGAGTTACAAATCAACGATCACCGGTAAAATAACGGAAAATAGACTTACCGATCTAAGGGGTATAAGCGTCAAAGTGATGTTCTTCTGGGTGAATATTCTTGAAGTTGTTAGGAAACACGATGACCTTGATTTCTCTGTTGGTGTTGCTTCTGCGGCTTTTCCTTTGGATAATTTCTTTGAATCTCCTCAGTGTGGTTGTGGTTTGGATTGTGATCGAATTAGAAAACTTAAACTCAATACTAAAAACCCTTCTCTTTCTTCTGCTTAA

Protein sequence

>MS.gene20747.t1

MTSPVVLLLLLTATALTVTGNPTAYEALRTYNFPPGILPIGVTSYELDKSSGNFRADLNRSCSFSLQGSYQLSYKSTITGKITENRLTDLRGISVKVMFFWVNILEVVRKHDDLDFSVGVASAAFPLDNFFESPQCGCGLDCDRIRKLKLNTKNPSLSSA