Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20797.t1 | XP_003591419.1 | 96.4 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 1.20E-102 | 382.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20797.t1 | G7IDR9 | 96.4 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 8.4e-103 | 382.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene20797 | 0.847011 | 1.51E-59 | -1.69E-46 |
| MS.gene051247 | MS.gene20797 | 0.807055 | 6.09E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene20797 | 0.800808 | 1.21E-48 | -1.69E-46 |
| MS.gene055465 | MS.gene20797 | 0.80369 | 3.09E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene20797 | 0.808096 | 3.66E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene20797 | 0.817449 | 3.28E-52 | -1.69E-46 |
| MS.gene055608 | MS.gene20797 | 0.809735 | 1.63E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20797.t1 | MTR_1g087230 | 96.354 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 9.45e-139 | 385 |
| MS.gene20797.t1 | MTR_7g109410 | 56.345 | 197 | 76 | 5 | 1 | 192 | 1 | 192 | 4.62e-72 | 216 |
| MS.gene20797.t1 | MTR_8g107420 | 27.841 | 176 | 112 | 4 | 1 | 176 | 1 | 161 | 4.90e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20797.t1 | AT4G02090 | 37.273 | 220 | 88 | 10 | 1 | 189 | 1 | 201 | 6.31e-32 | 114 |
Find 41 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTTGAATAGGCAGATTTC+AGG | 0.248523 | 1.2:+65533829 | MS.gene20797:CDS |
| GCCTAGCTTGGATACTATTA+AGG | 0.311308 | 1.2:+65533864 | MS.gene20797:CDS |
| GTTCTTATAAGTTTCTTCCT+TGG | 0.362756 | 1.2:+65533680 | MS.gene20797:CDS |
| ATCTGCCTATTCAAATAATC+TGG | 0.371640 | 1.2:-65533823 | None:intergenic |
| GGAAGAGGTGATGGAGGAGA+AGG | 0.379095 | 1.2:+65533750 | MS.gene20797:CDS |
| TCTTCGCCGCTTAAACCAAT+AGG | 0.383885 | 1.2:-65533601 | None:intergenic |
| ATAGGCTTCCCTTGCTTCAC+TGG | 0.390684 | 1.2:-65533583 | None:intergenic |
| GGAGAATTTGCCGGACATAT+TGG | 0.397303 | 1.2:+65533792 | MS.gene20797:CDS |
| GTGAAGCAAGGGAAGCCTAT+TGG | 0.415059 | 1.2:+65533586 | MS.gene20797:CDS |
| TTTCGTCCTTCAGATTCAAC+AGG | 0.424376 | 1.2:+65533379 | MS.gene20797:CDS |
| ATCTCTAGCTTCTTGTCAGC+AGG | 0.426386 | 1.2:-65533538 | None:intergenic |
| GGAGGCCAGATTATTTGAAT+AGG | 0.439315 | 1.2:+65533818 | MS.gene20797:CDS |
| AGCTTCTTGTCAGCAGGTAA+TGG | 0.466025 | 1.2:-65533532 | None:intergenic |
| ATGTCATAACAGTGAAATAG+TGG | 0.471468 | 1.2:+65533714 | MS.gene20797:CDS |
| GAAGAGGTGATGGAGGAGAA+GGG | 0.490391 | 1.2:+65533751 | MS.gene20797:CDS |
| GAAAGGGTGGAAGCCTAGCT+TGG | 0.506451 | 1.2:+65533852 | MS.gene20797:CDS |
| TCGGAGGAAGGAAGAGGTGA+TGG | 0.513945 | 1.2:+65533741 | MS.gene20797:CDS |
| TTGTCAGCAGGTAATGGTGT+TGG | 0.520247 | 1.2:-65533526 | None:intergenic |
| ATTTGAATAGGCAGATTTCA+GGG | 0.521145 | 1.2:+65533830 | MS.gene20797:CDS |
| AATAGGCAGATTTCAGGGAA+AGG | 0.530769 | 1.2:+65533835 | MS.gene20797:CDS |
| GGCCTCCCTTCCAATATGTC+CGG | 0.533242 | 1.2:-65533802 | None:intergenic |
| TGATGCTTCCAGTGAAGCAA+GGG | 0.535617 | 1.2:+65533575 | MS.gene20797:CDS |
| ATAGGCAGATTTCAGGGAAA+GGG | 0.549284 | 1.2:+65533836 | MS.gene20797:CDS |
| AACAGTGGCAGAGCTAATGA+TGG | 0.551225 | 1.2:+65533450 | MS.gene20797:CDS |
| GGAGCCTCGGAGGAAGGAAG+AGG | 0.553960 | 1.2:+65533735 | MS.gene20797:CDS |
| TTGATGCTTCCAGTGAAGCA+AGG | 0.559013 | 1.2:+65533574 | MS.gene20797:CDS |
| ACTTTACCTGTTGAATCTGA+AGG | 0.560942 | 1.2:-65533385 | None:intergenic |
| AATTTGCCGGACATATTGGA+AGG | 0.563693 | 1.2:+65533796 | MS.gene20797:CDS |
| TCCTTAATAGTATCCAAGCT+AGG | 0.569966 | 1.2:-65533865 | None:intergenic |
| AATAGTGGAGCCTCGGAGGA+AGG | 0.571841 | 1.2:+65533729 | MS.gene20797:CDS |
| GGGAAGCCTATTGGTTTAAG+CGG | 0.581568 | 1.2:+65533595 | MS.gene20797:CDS |
| ACAGTGAAATAGTGGAGCCT+CGG | 0.582711 | 1.2:+65533722 | MS.gene20797:CDS |
| GAGGAAGGAAGAGGTGATGG+AGG | 0.589938 | 1.2:+65533744 | MS.gene20797:CDS |
| GGCAGATTTCAGGGAAAGGG+TGG | 0.595138 | 1.2:+65533839 | MS.gene20797:CDS |
| AATTAACCATCAACAAAACT+CGG | 0.602000 | 1.2:-65533630 | None:intergenic |
| TGCCGGACATATTGGAAGGG+AGG | 0.607569 | 1.2:+65533800 | MS.gene20797:CDS |
| GTGAAATAGTGGAGCCTCGG+AGG | 0.619098 | 1.2:+65533725 | MS.gene20797:CDS |
| TGACATAAATTACTAAACCA+AGG | 0.652368 | 1.2:-65533697 | None:intergenic |
| ATTTGCCGGACATATTGGAA+GGG | 0.657578 | 1.2:+65533797 | MS.gene20797:CDS |
| ATCACCTCTTCCTTCCTCCG+AGG | 0.665878 | 1.2:-65533739 | None:intergenic |
| ATTTGATGAAGCAATAACAG+TGG | 0.710910 | 1.2:+65533435 | MS.gene20797:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTAACCATCAACAAAACT+CGG | - | chr1.2:65533633-65533652 | None:intergenic | 25.0% |
| ! | TGACATAAATTACTAAACCA+AGG | - | chr1.2:65533700-65533719 | None:intergenic | 25.0% |
| ! | TTGAGAAAAAACATACTCAT+TGG | + | chr1.2:65533896-65533915 | MS.gene20797:CDS | 25.0% |
| ATCTGCCTATTCAAATAATC+TGG | - | chr1.2:65533826-65533845 | None:intergenic | 30.0% | |
| ATGTCATAACAGTGAAATAG+TGG | + | chr1.2:65533714-65533733 | MS.gene20797:CDS | 30.0% | |
| ATTTGAATAGGCAGATTTCA+GGG | + | chr1.2:65533830-65533849 | MS.gene20797:CDS | 30.0% | |
| ATTTGATGAAGCAATAACAG+TGG | + | chr1.2:65533435-65533454 | MS.gene20797:CDS | 30.0% | |
| GTTCTTATAAGTTTCTTCCT+TGG | + | chr1.2:65533680-65533699 | MS.gene20797:CDS | 30.0% | |
| TATTTGAATAGGCAGATTTC+AGG | + | chr1.2:65533829-65533848 | MS.gene20797:CDS | 30.0% | |
| ! | GGTAAAGTAGTTTTCTTAGA+TGG | + | chr1.2:65533400-65533419 | MS.gene20797:CDS | 30.0% |
| ACTTTACCTGTTGAATCTGA+AGG | - | chr1.2:65533388-65533407 | None:intergenic | 35.0% | |
| ATTCAGCTGTGAACCAAAAA+AGG | + | chr1.2:65533503-65533522 | MS.gene20797:CDS | 35.0% | |
| TCCTTAATAGTATCCAAGCT+AGG | - | chr1.2:65533868-65533887 | None:intergenic | 35.0% | |
| !!! | AACTCAACCACTACTTTTTG+TGG | - | chr1.2:65533481-65533500 | None:intergenic | 35.0% |
| AATAGGCAGATTTCAGGGAA+AGG | + | chr1.2:65533835-65533854 | MS.gene20797:CDS | 40.0% | |
| AATTTGCCGGACATATTGGA+AGG | + | chr1.2:65533796-65533815 | MS.gene20797:CDS | 40.0% | |
| ATAGGCAGATTTCAGGGAAA+GGG | + | chr1.2:65533836-65533855 | MS.gene20797:CDS | 40.0% | |
| ATTTGCCGGACATATTGGAA+GGG | + | chr1.2:65533797-65533816 | MS.gene20797:CDS | 40.0% | |
| GCCTAGCTTGGATACTATTA+AGG | + | chr1.2:65533864-65533883 | MS.gene20797:CDS | 40.0% | |
| GGAGGCCAGATTATTTGAAT+AGG | + | chr1.2:65533818-65533837 | MS.gene20797:CDS | 40.0% | |
| GGATCATCCACAAAAAGTAG+TGG | + | chr1.2:65533471-65533490 | MS.gene20797:CDS | 40.0% | |
| TTTCGTCCTTCAGATTCAAC+AGG | + | chr1.2:65533379-65533398 | MS.gene20797:CDS | 40.0% | |
| !! | CGATTTTTCGGAGAATTTGC+CGG | + | chr1.2:65533783-65533802 | MS.gene20797:CDS | 40.0% |
| !!! | GTAATGGTGTTGGCCTTTTT+TGG | - | chr1.2:65533519-65533538 | None:intergenic | 40.0% |
| AACAGTGGCAGAGCTAATGA+TGG | + | chr1.2:65533450-65533469 | MS.gene20797:CDS | 45.0% | |
| AGCTTCTTGTCAGCAGGTAA+TGG | - | chr1.2:65533535-65533554 | None:intergenic | 45.0% | |
| GGAGAATTTGCCGGACATAT+TGG | + | chr1.2:65533792-65533811 | MS.gene20797:CDS | 45.0% | |
| TCTTCGCCGCTTAAACCAAT+AGG | - | chr1.2:65533604-65533623 | None:intergenic | 45.0% | |
| TGATGCTTCCAGTGAAGCAA+GGG | + | chr1.2:65533575-65533594 | MS.gene20797:CDS | 45.0% | |
| TTGATGCTTCCAGTGAAGCA+AGG | + | chr1.2:65533574-65533593 | MS.gene20797:CDS | 45.0% | |
| ! | ACAGTGAAATAGTGGAGCCT+CGG | + | chr1.2:65533722-65533741 | MS.gene20797:CDS | 45.0% |
| ! | ATCTCTAGCTTCTTGTCAGC+AGG | - | chr1.2:65533541-65533560 | None:intergenic | 45.0% |
| ! | GGGAGAGAGACACGATTTTT+CGG | + | chr1.2:65533771-65533790 | MS.gene20797:CDS | 45.0% |
| ! | TTGTCAGCAGGTAATGGTGT+TGG | - | chr1.2:65533529-65533548 | None:intergenic | 45.0% |
| !! | GGGAAGCCTATTGGTTTAAG+CGG | + | chr1.2:65533595-65533614 | MS.gene20797:CDS | 45.0% |
| !!! | AACTCGCCGAGTTTTGTTGA+TGG | + | chr1.2:65533624-65533643 | MS.gene20797:CDS | 45.0% |
| ATAGGCTTCCCTTGCTTCAC+TGG | - | chr1.2:65533586-65533605 | None:intergenic | 50.0% | |
| GTGAAGCAAGGGAAGCCTAT+TGG | + | chr1.2:65533586-65533605 | MS.gene20797:CDS | 50.0% | |
| !! | GAAGAGGTGATGGAGGAGAA+GGG | + | chr1.2:65533751-65533770 | MS.gene20797:CDS | 50.0% |
| ATCACCTCTTCCTTCCTCCG+AGG | - | chr1.2:65533742-65533761 | None:intergenic | 55.0% | |
| GAAAGGGTGGAAGCCTAGCT+TGG | + | chr1.2:65533852-65533871 | MS.gene20797:CDS | 55.0% | |
| GGCAGATTTCAGGGAAAGGG+TGG | + | chr1.2:65533839-65533858 | MS.gene20797:CDS | 55.0% | |
| GGCCTCCCTTCCAATATGTC+CGG | - | chr1.2:65533805-65533824 | None:intergenic | 55.0% | |
| TCGGAGGAAGGAAGAGGTGA+TGG | + | chr1.2:65533741-65533760 | MS.gene20797:CDS | 55.0% | |
| TGCCGGACATATTGGAAGGG+AGG | + | chr1.2:65533800-65533819 | MS.gene20797:CDS | 55.0% | |
| ! | AATAGTGGAGCCTCGGAGGA+AGG | + | chr1.2:65533729-65533748 | MS.gene20797:CDS | 55.0% |
| ! | GAGGAAGGAAGAGGTGATGG+AGG | + | chr1.2:65533744-65533763 | MS.gene20797:CDS | 55.0% |
| ! | GTGAAATAGTGGAGCCTCGG+AGG | + | chr1.2:65533725-65533744 | MS.gene20797:CDS | 55.0% |
| !! | GGAAGAGGTGATGGAGGAGA+AGG | + | chr1.2:65533750-65533769 | MS.gene20797:CDS | 55.0% |
| GGAGCCTCGGAGGAAGGAAG+AGG | + | chr1.2:65533735-65533754 | MS.gene20797:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 65533361 | 65533939 | 65533361 | ID=MS.gene20797 |
| chr1.2 | mRNA | 65533361 | 65533939 | 65533361 | ID=MS.gene20797.t1;Parent=MS.gene20797 |
| chr1.2 | exon | 65533361 | 65533939 | 65533361 | ID=MS.gene20797.t1.exon1;Parent=MS.gene20797.t1 |
| chr1.2 | CDS | 65533361 | 65533939 | 65533361 | ID=cds.MS.gene20797.t1;Parent=MS.gene20797.t1 |
| Gene Sequence |
| Protein sequence |
