Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053164.t1 | XP_013454569.1 | 89 | 173 | 10 | 1 | 1 | 173 | 248 | 411 | 7.80E-82 | 313.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053164.t1 | Q9FLD7 | 38.2 | 170 | 94 | 2 | 1 | 169 | 288 | 447 | 1.5e-28 | 127.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053164.t1 | A0A072URV1 | 89.0 | 173 | 10 | 1 | 1 | 173 | 248 | 411 | 5.6e-82 | 313.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene053164 | 0.803257 | 3.80E-49 | -1.69E-46 |
| MS.gene050657 | MS.gene053164 | 0.813798 | 2.13E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene053164 | 0.803246 | 3.82E-49 | -1.69E-46 |
| MS.gene051388 | MS.gene053164 | 0.811914 | 5.52E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene053164 | 0.813436 | 2.56E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene053164 | 0.813851 | 2.08E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene053164 | 0.814944 | 1.19E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene053581 | 0.804699 | 1.91E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene054584 | 0.823679 | 1.22E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene054801 | 0.80545 | 1.33E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene055465 | 0.824406 | 8.21E-54 | -1.69E-46 |
| MS.gene053164 | MS.gene055466 | 0.82996 | 3.84E-55 | -1.69E-46 |
| MS.gene053164 | MS.gene055468 | 0.82206 | 2.90E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene055469 | 0.803775 | 2.97E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene058339 | 0.813882 | 2.04E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene058625 | 0.802829 | 4.66E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene059625 | 0.80752 | 4.86E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene06133 | 0.804045 | 2.61E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene062042 | 0.800997 | 1.11E-48 | -1.69E-46 |
| MS.gene053164 | MS.gene062043 | 0.823252 | 1.53E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene063058 | 0.802673 | 5.02E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene063060 | 0.809034 | 2.31E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene063149 | 0.806333 | 8.66E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene064321 | 0.802337 | 5.89E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene064336 | 0.809285 | 2.04E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene065908 | 0.80568 | 1.19E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene06598 | 0.812444 | 4.23E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene066335 | 0.818784 | 1.64E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene066436 | 0.827833 | 1.26E-54 | -1.69E-46 |
| MS.gene053164 | MS.gene066784 | 0.806576 | 7.69E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene068388 | 0.804515 | 2.08E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene068924 | 0.809 | 2.35E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene069410 | 0.81035 | 1.20E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene072116 | 0.80093 | 1.14E-48 | -1.69E-46 |
| MS.gene053164 | MS.gene073083 | 0.818948 | 1.50E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene074206 | 0.809056 | 2.28E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene074519 | 0.809817 | 1.57E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene07876 | 0.809878 | 1.52E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene20797 | 0.800808 | 1.21E-48 | -1.69E-46 |
| MS.gene053164 | MS.gene20847 | 0.814675 | 1.37E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene20903 | 0.801972 | 7.00E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene21826 | 0.8158 | 7.68E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene22177 | 0.818439 | 1.96E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene22707 | 0.829606 | 4.69E-55 | -1.69E-46 |
| MS.gene053164 | MS.gene23571 | 0.809538 | 1.80E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene24445 | 0.823167 | 1.60E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene24736 | 0.816457 | 5.48E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene25810 | 0.819829 | 9.46E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene25816 | 0.803812 | 2.92E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene26380 | 0.81954 | 1.10E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene26659 | 0.82218 | 2.72E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene27700 | 0.812169 | 4.85E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene29022 | 0.82018 | 7.86E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene29489 | 0.805479 | 1.31E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene29807 | 0.806183 | 9.31E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene30350 | 0.805492 | 1.30E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene30530 | 0.825919 | 3.60E-54 | -1.69E-46 |
| MS.gene053164 | MS.gene30739 | 0.81209 | 5.05E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene30882 | 0.808143 | 3.58E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene31231 | 0.80593 | 1.05E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene31347 | 0.817147 | 3.83E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene31433 | 0.839622 | 1.42E-57 | -1.69E-46 |
| MS.gene053164 | MS.gene32555 | 0.801484 | 8.81E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene33598 | 0.806873 | 6.66E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene34180 | 0.827521 | 1.49E-54 | -1.69E-46 |
| MS.gene053164 | MS.gene34286 | 0.819593 | 1.07E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene35375 | 0.803691 | 3.09E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene35575 | 0.806482 | 8.05E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene35631 | 0.819217 | 1.30E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene38303 | 0.82392 | 1.07E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene38561 | 0.81124 | 7.73E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene41395 | 0.802197 | 6.29E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene41757 | 0.836546 | 8.78E-57 | -1.69E-46 |
| MS.gene053164 | MS.gene43075 | 0.821089 | 4.86E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene44427 | 0.818009 | 2.45E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene45425 | 0.808185 | 3.51E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene45624 | 0.839624 | 1.42E-57 | -1.69E-46 |
| MS.gene053164 | MS.gene48814 | 0.820976 | 5.16E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene49467 | 0.829667 | 4.53E-55 | -1.69E-46 |
| MS.gene053164 | MS.gene50163 | 0.809644 | 1.71E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene51818 | 0.803262 | 3.79E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene52525 | 0.80508 | 1.59E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene53321 | 0.813239 | 2.83E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene55406 | 0.808709 | 2.71E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene56748 | 0.810594 | 1.07E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene58547 | 0.80923 | 2.10E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene58802 | 0.806357 | 8.55E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene58992 | 0.84096 | 6.34E-58 | -1.69E-46 |
| MS.gene053164 | MS.gene60163 | 0.821739 | 3.44E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene60171 | 0.801884 | 7.29E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene61720 | 0.800013 | 1.76E-48 | -1.69E-46 |
| MS.gene053164 | MS.gene62806 | 0.811408 | 7.11E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene64671 | 0.807308 | 5.39E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene65165 | 0.816101 | 6.58E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene65172 | 0.858869 | 6.13E-63 | -1.69E-46 |
| MS.gene053164 | MS.gene67298 | 0.802179 | 6.34E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene67299 | 0.80228 | 6.05E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene68204 | 0.803453 | 3.46E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene69510 | 0.816678 | 4.89E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene70730 | 0.819371 | 1.20E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene72986 | 0.809412 | 1.92E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene73663 | 0.806017 | 1.01E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene74364 | 0.80315 | 4.00E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene75719 | 0.807723 | 4.40E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene76734 | 0.805021 | 1.63E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene77370 | 0.869965 | 2.08E-66 | -1.69E-46 |
| MS.gene053164 | MS.gene80732 | 0.804025 | 2.63E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene80853 | 0.815232 | 1.03E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene81370 | 0.823659 | 1.23E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene81678 | 0.805695 | 1.18E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene82701 | 0.804306 | 2.30E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene82929 | 0.815219 | 1.03E-51 | -1.69E-46 |
| MS.gene053164 | MS.gene83055 | 0.804393 | 2.21E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene84426 | 0.807584 | 4.71E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene84994 | 0.801543 | 8.57E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene86140 | 0.819089 | 1.39E-52 | -1.69E-46 |
| MS.gene053164 | MS.gene90022 | 0.804088 | 2.55E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene90610 | 0.808345 | 3.24E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene90646 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene92196 | 0.820801 | 5.66E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene93198 | 0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene94683 | 0.823878 | 1.09E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene95401 | 0.805075 | 1.59E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene95562 | 0.835053 | 2.10E-56 | -1.69E-46 |
| MS.gene053164 | MS.gene95624 | 0.836022 | 1.19E-56 | -1.69E-46 |
| MS.gene053164 | MS.gene96598 | 0.806418 | 8.31E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene98087 | 0.800362 | 1.49E-48 | -1.69E-46 |
| MS.gene053164 | MS.gene98089 | 0.800408 | 1.46E-48 | -1.69E-46 |
| MS.gene053164 | MS.gene98394 | 0.80216 | 6.40E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene98395 | 0.809396 | 1.93E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene98990 | 0.802966 | 4.37E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053164.t1 | MTR_4g008010 | 89.017 | 173 | 10 | 1 | 1 | 173 | 248 | 411 | 5.78e-109 | 317 |
| MS.gene053164.t1 | MTR_4g022240 | 39.412 | 170 | 92 | 2 | 1 | 169 | 260 | 419 | 1.17e-35 | 128 |
| MS.gene053164.t1 | MTR_1g046740 | 36.310 | 168 | 95 | 1 | 1 | 168 | 283 | 438 | 6.41e-34 | 124 |
| MS.gene053164.t1 | MTR_0072s0020 | 37.278 | 169 | 93 | 4 | 1 | 168 | 276 | 432 | 7.16e-33 | 121 |
| MS.gene053164.t1 | MTR_1g108280 | 37.427 | 171 | 95 | 4 | 1 | 169 | 237 | 397 | 7.63e-33 | 120 |
| MS.gene053164.t1 | MTR_6g027550 | 36.686 | 169 | 99 | 2 | 2 | 169 | 156 | 317 | 1.64e-31 | 115 |
| MS.gene053164.t1 | MTR_6g011080 | 36.686 | 169 | 96 | 2 | 1 | 168 | 274 | 432 | 4.98e-31 | 116 |
| MS.gene053164.t1 | MTR_0662s0030 | 40.769 | 130 | 76 | 1 | 1 | 129 | 163 | 292 | 6.79e-31 | 113 |
| MS.gene053164.t1 | MTR_4g114140 | 41.406 | 128 | 74 | 1 | 1 | 127 | 262 | 389 | 1.95e-30 | 114 |
| MS.gene053164.t1 | MTR_7g086720 | 35.503 | 169 | 98 | 2 | 2 | 169 | 241 | 399 | 2.69e-30 | 114 |
| MS.gene053164.t1 | MTR_2g085300 | 31.176 | 170 | 101 | 2 | 1 | 169 | 270 | 424 | 1.39e-23 | 96.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053164.t1 | AT5G39990 | 38.235 | 170 | 94 | 2 | 1 | 169 | 288 | 447 | 2.33e-35 | 128 |
| MS.gene053164.t1 | AT1G03520 | 36.686 | 169 | 94 | 4 | 1 | 168 | 201 | 357 | 6.75e-32 | 117 |
| MS.gene053164.t1 | AT1G03520 | 36.686 | 169 | 94 | 4 | 1 | 168 | 290 | 446 | 2.71e-31 | 117 |
| MS.gene053164.t1 | AT1G71070 | 36.471 | 170 | 97 | 2 | 1 | 169 | 236 | 395 | 2.77e-31 | 116 |
| MS.gene053164.t1 | AT4G03340 | 36.095 | 169 | 95 | 4 | 1 | 168 | 268 | 424 | 5.26e-30 | 113 |
| MS.gene053164.t1 | AT4G03340 | 36.095 | 169 | 95 | 4 | 1 | 168 | 291 | 447 | 7.57e-30 | 113 |
| MS.gene053164.t1 | AT5G15050 | 34.118 | 170 | 101 | 2 | 1 | 169 | 275 | 434 | 1.01e-29 | 113 |
| MS.gene053164.t1 | AT4G27480 | 32.941 | 170 | 98 | 2 | 1 | 169 | 267 | 421 | 7.23e-29 | 110 |
| MS.gene053164.t1 | AT4G27480 | 32.941 | 170 | 98 | 2 | 1 | 169 | 267 | 421 | 7.23e-29 | 110 |
| MS.gene053164.t1 | AT4G27480 | 32.941 | 170 | 98 | 2 | 1 | 169 | 267 | 421 | 7.23e-29 | 110 |
| MS.gene053164.t1 | AT3G15350 | 34.302 | 172 | 95 | 3 | 1 | 169 | 268 | 424 | 7.18e-27 | 105 |
| MS.gene053164.t1 | AT3G15350 | 34.302 | 172 | 95 | 3 | 1 | 169 | 268 | 424 | 7.18e-27 | 105 |
| MS.gene053164.t1 | AT3G24040 | 35.503 | 169 | 98 | 3 | 1 | 168 | 256 | 414 | 7.44e-27 | 105 |
| MS.gene053164.t1 | AT2G37585 | 34.320 | 169 | 83 | 2 | 1 | 169 | 244 | 384 | 2.99e-26 | 103 |
| MS.gene053164.t1 | AT1G53100 | 31.176 | 170 | 100 | 3 | 1 | 168 | 268 | 422 | 4.60e-26 | 102 |
| MS.gene053164.t1 | AT1G53100 | 30.864 | 162 | 95 | 3 | 1 | 160 | 268 | 414 | 8.90e-24 | 96.7 |
| MS.gene053164.t1 | AT3G03690 | 42.000 | 100 | 57 | 1 | 2 | 100 | 241 | 340 | 2.52e-20 | 86.7 |
Find 29 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCTTAATAGAACTTTAAA+TGG | 0.201353 | 4.2:-91956305 | MS.gene053164:CDS |
| TCTAGAGAAAATTGATGATT+TGG | 0.283786 | 4.2:-91956327 | MS.gene053164:CDS |
| ATCAAACTTAGAACTCTATT+AGG | 0.391070 | 4.2:-91956152 | MS.gene053164:CDS |
| CAGCAATGTGGCTTATCCTA+TGG | 0.406519 | 4.2:-91956537 | MS.gene053164:CDS |
| TTAGGTGAAATTGTTAATAG+TGG | 0.407486 | 4.2:-91956134 | MS.gene053164:CDS |
| GTTGTAAAGCCAGGATTGTT+TGG | 0.420135 | 4.2:-91956176 | MS.gene053164:CDS |
| ATGGATTTGTTCAAGGAGAA+TGG | 0.425534 | 4.2:-91956286 | MS.gene053164:CDS |
| TATGATACAATGATGGAAAC+TGG | 0.434308 | 4.2:-91956389 | MS.gene053164:CDS |
| TTTATGGAGTATTGTGTGAA+TGG | 0.438026 | 4.2:-91956596 | MS.gene053164:CDS |
| GAAAGTGTGTGTTTAGTATC+TGG | 0.446446 | 4.2:-91956209 | MS.gene053164:CDS |
| GCTGTATTTGCACGTCCATT+TGG | 0.459130 | 4.2:-91956365 | MS.gene053164:CDS |
| TGAAATCTTGGGAGTTACAT+AGG | 0.459159 | 4.2:+91956495 | None:intergenic |
| AACATTGATGTTGTAAAGCC+AGG | 0.461968 | 4.2:-91956185 | MS.gene053164:CDS |
| ACTTTAAATGGATTTGTTCA+AGG | 0.464729 | 4.2:-91956293 | MS.gene053164:CDS |
| ACAAGATCACCTTCACCAAA+TGG | 0.480206 | 4.2:+91956350 | None:intergenic |
| AGTTTGATGCCAAACAATCC+TGG | 0.484819 | 4.2:+91956167 | None:intergenic |
| TGGAGTATTGTGTGAATGGA+TGG | 0.505200 | 4.2:-91956592 | MS.gene053164:CDS |
| TAGGTGAAATTGTTAATAGT+GGG | 0.525063 | 4.2:-91956133 | MS.gene053164:CDS |
| ACACTAATTGCAATTGACAT+TGG | 0.525426 | 4.2:+91956096 | None:intergenic |
| GTCACACTATGATACAATGA+TGG | 0.547246 | 4.2:-91956396 | MS.gene053164:CDS |
| TCAAGTAATTGAGATTCTGA+TGG | 0.565400 | 4.2:+91956422 | None:intergenic |
| TACAAAGGTTATAGAAGATG+AGG | 0.566651 | 4.2:-91956231 | MS.gene053164:CDS |
| AGAGATAAATAAAACTACAA+AGG | 0.603372 | 4.2:-91956246 | MS.gene053164:CDS |
| GTGTGGAAATAAGTTTCCAT+AGG | 0.614317 | 4.2:+91956521 | None:intergenic |
| TTTGCACGTCCATTTGGTGA+AGG | 0.615479 | 4.2:-91956359 | MS.gene053164:CDS |
| GGAGTATTGTGTGAATGGAT+GGG | 0.648311 | 4.2:-91956591 | MS.gene053164:CDS |
| AATTAGATTATTGTCCACTG+TGG | 0.680300 | 4.2:+91956466 | None:intergenic |
| TATGGAAACTTATTTCCACA+CGG | 0.684317 | 4.2:-91956519 | MS.gene053164:CDS |
| GGGAGTTACATAGGACCGTG+TGG | 0.724135 | 4.2:+91956504 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAGATAAATAAAACTACAA+AGG | - | chr4.2:91956441-91956460 | MS.gene053164:CDS | 20.0% |
| ! | AAAAACATTAGTAGCTTTCT+TGG | + | chr4.2:91956130-91956149 | None:intergenic | 25.0% |
| ! | ATCAAACTTAGAACTCTATT+AGG | - | chr4.2:91956535-91956554 | MS.gene053164:CDS | 25.0% |
| ! | GCTCTTAATAGAACTTTAAA+TGG | - | chr4.2:91956382-91956401 | MS.gene053164:CDS | 25.0% |
| ! | TAGGTGAAATTGTTAATAGT+GGG | - | chr4.2:91956554-91956573 | MS.gene053164:CDS | 25.0% |
| ! | TTAGGTGAAATTGTTAATAG+TGG | - | chr4.2:91956553-91956572 | MS.gene053164:CDS | 25.0% |
| !! | ACTTTAAATGGATTTGTTCA+AGG | - | chr4.2:91956394-91956413 | MS.gene053164:CDS | 25.0% |
| !! | TCTAGAGAAAATTGATGATT+TGG | - | chr4.2:91956360-91956379 | MS.gene053164:CDS | 25.0% |
| AATTAGATTATTGTCCACTG+TGG | + | chr4.2:91956224-91956243 | None:intergenic | 30.0% | |
| ACACTAATTGCAATTGACAT+TGG | + | chr4.2:91956594-91956613 | None:intergenic | 30.0% | |
| TACAAAGGTTATAGAAGATG+AGG | - | chr4.2:91956456-91956475 | MS.gene053164:CDS | 30.0% | |
| TATGGAAACTTATTTCCACA+CGG | - | chr4.2:91956168-91956187 | MS.gene053164:CDS | 30.0% | |
| TCAAGTAATTGAGATTCTGA+TGG | + | chr4.2:91956268-91956287 | None:intergenic | 30.0% | |
| ! | TATGATACAATGATGGAAAC+TGG | - | chr4.2:91956298-91956317 | MS.gene053164:CDS | 30.0% |
| !! | ACTAATGTTTTTCAGCAATG+TGG | - | chr4.2:91956138-91956157 | MS.gene053164:CDS | 30.0% |
| !!! | TGTGGTGTTTTTGAAATCTT+GGG | + | chr4.2:91956206-91956225 | None:intergenic | 30.0% |
| AACATTGATGTTGTAAAGCC+AGG | - | chr4.2:91956502-91956521 | MS.gene053164:CDS | 35.0% | |
| AGATTTCAAAAACACCACAG+TGG | - | chr4.2:91956207-91956226 | MS.gene053164:CDS | 35.0% | |
| GTCACACTATGATACAATGA+TGG | - | chr4.2:91956291-91956310 | MS.gene053164:CDS | 35.0% | |
| GTGTGGAAATAAGTTTCCAT+AGG | + | chr4.2:91956169-91956188 | None:intergenic | 35.0% | |
| TGAAATCTTGGGAGTTACAT+AGG | + | chr4.2:91956195-91956214 | None:intergenic | 35.0% | |
| ! | ATGGATTTGTTCAAGGAGAA+TGG | - | chr4.2:91956401-91956420 | MS.gene053164:CDS | 35.0% |
| ! | GAAAGTGTGTGTTTAGTATC+TGG | - | chr4.2:91956478-91956497 | MS.gene053164:CDS | 35.0% |
| !!! | CTGTGGTGTTTTTGAAATCT+TGG | + | chr4.2:91956207-91956226 | None:intergenic | 35.0% |
| ACAAGATCACCTTCACCAAA+TGG | + | chr4.2:91956340-91956359 | None:intergenic | 40.0% | |
| AGTTTGATGCCAAACAATCC+TGG | + | chr4.2:91956523-91956542 | None:intergenic | 40.0% | |
| GTTGTAAAGCCAGGATTGTT+TGG | - | chr4.2:91956511-91956530 | MS.gene053164:CDS | 40.0% | |
| !! | GGAGTATTGTGTGAATGGAT+GGG | - | chr4.2:91956096-91956115 | MS.gene053164:CDS | 40.0% |
| !! | TGGAGTATTGTGTGAATGGA+TGG | - | chr4.2:91956095-91956114 | MS.gene053164:CDS | 40.0% |
| CAGCAATGTGGCTTATCCTA+TGG | - | chr4.2:91956150-91956169 | MS.gene053164:CDS | 45.0% | |
| GCTGTATTTGCACGTCCATT+TGG | - | chr4.2:91956322-91956341 | MS.gene053164:CDS | 45.0% | |
| ! | TTTGCACGTCCATTTGGTGA+AGG | - | chr4.2:91956328-91956347 | MS.gene053164:CDS | 45.0% |
| GGGAGTTACATAGGACCGTG+TGG | + | chr4.2:91956186-91956205 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 91956094 | 91956615 | 91956094 | ID=MS.gene053164 |
| chr4.2 | mRNA | 91956094 | 91956615 | 91956094 | ID=MS.gene053164.t1;Parent=MS.gene053164 |
| chr4.2 | exon | 91956094 | 91956615 | 91956094 | ID=MS.gene053164.t1.exon1;Parent=MS.gene053164.t1 |
| chr4.2 | CDS | 91956094 | 91956615 | 91956094 | ID=cds.MS.gene053164.t1;Parent=MS.gene053164.t1 |
| Gene Sequence |
| Protein sequence |
