Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21034.t1 | KEH43112.1 | 94.7 | 57 | 3 | 0 | 1 | 57 | 1 | 57 | 4.80E-20 | 106.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21034.t1 | A0A072VMN8 | 94.7 | 57 | 3 | 0 | 1 | 57 | 1 | 57 | 3.4e-20 | 106.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene21034 | 0.822995 | 1.76E-53 | -1.69E-46 |
| MS.gene050020 | MS.gene21034 | 0.808014 | 3.81E-50 | -1.69E-46 |
| MS.gene050277 | MS.gene21034 | 0.827165 | 1.82E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene21034 | 0.804505 | 2.09E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene21034 | 0.83182 | 1.34E-55 | -1.69E-46 |
| MS.gene051328 | MS.gene21034 | 0.80305 | 4.19E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene21034 | 0.802179 | 6.34E-49 | -1.69E-46 |
| MS.gene057234 | MS.gene21034 | 0.814143 | 1.79E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene21034 | 0.806652 | 7.41E-50 | -1.69E-46 |
| MS.gene058055 | MS.gene21034 | 0.817992 | 2.47E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene21034 | 0.806128 | 9.56E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21034.t1 | MTR_1g084170 | 94.737 | 57 | 3 | 0 | 1 | 57 | 1 | 57 | 5.87e-34 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTCTTCCTCCTGTCCTA+TGG | 0.455567 | 1.4:+68851361 | MS.gene21034:CDS |
| ATTTGTGTGGGGAATCGTTA+CGG | 0.507320 | 1.4:+68851306 | MS.gene21034:CDS |
| CATTGTTGATGAATTTGTGT+GGG | 0.545323 | 1.4:+68851294 | MS.gene21034:CDS |
| GATTCAGATGGTGCAGCCAT+AGG | 0.548895 | 1.4:-68851377 | None:intergenic |
| AGATGGTGCAGCCATAGGAC+AGG | 0.558193 | 1.4:-68851372 | None:intergenic |
| CCATTGTTGATGAATTTGTG+TGG | 0.566731 | 1.4:+68851293 | MS.gene21034:CDS |
| TGATGGCATCAGTATTCCGT+AGG | 0.577997 | 1.4:+68851221 | None:intergenic |
| TGGTGCAGCCATAGGACAGG+AGG | 0.583739 | 1.4:-68851369 | None:intergenic |
| ATTATCAGCAACGCGGTGGT+CGG | 0.584473 | 1.4:+68851255 | MS.gene21034:CDS |
| CCACACAAATTCATCAACAA+TGG | 0.585426 | 1.4:-68851293 | None:intergenic |
| ATAATTTCATCATTACCCTA+CGG | 0.597434 | 1.4:-68851237 | None:intergenic |
| TGAAATTATCAGCAACGCGG+TGG | 0.607476 | 1.4:+68851251 | MS.gene21034:CDS |
| ATTGTTGATGAATTTGTGTG+GGG | 0.644928 | 1.4:+68851295 | MS.gene21034:CDS |
| GATGGCATCAGTATTCCGTA+GGG | 0.661914 | 1.4:+68851222 | None:intergenic |
| TGTTATGTATCAGATTCAGA+TGG | 0.701986 | 1.4:-68851389 | None:intergenic |
| TGATGAAATTATCAGCAACG+CGG | 0.808577 | 1.4:+68851248 | MS.gene21034:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAATTTCATCATTACCCTA+CGG | - | chr1.4:68851240-68851259 | None:intergenic | 25.0% |
| ! | ATTGTTGATGAATTTGTGTG+GGG | + | chr1.4:68851295-68851314 | MS.gene21034:CDS | 30.0% |
| ! | CATTGTTGATGAATTTGTGT+GGG | + | chr1.4:68851294-68851313 | MS.gene21034:CDS | 30.0% |
| CCACACAAATTCATCAACAA+TGG | - | chr1.4:68851296-68851315 | None:intergenic | 35.0% | |
| TGATGAAATTATCAGCAACG+CGG | + | chr1.4:68851248-68851267 | MS.gene21034:CDS | 35.0% | |
| ! | CCATTGTTGATGAATTTGTG+TGG | + | chr1.4:68851293-68851312 | MS.gene21034:CDS | 35.0% |
| ATTTGTGTGGGGAATCGTTA+CGG | + | chr1.4:68851306-68851325 | MS.gene21034:CDS | 40.0% | |
| TGAAATTATCAGCAACGCGG+TGG | + | chr1.4:68851251-68851270 | MS.gene21034:CDS | 45.0% | |
| ATTATCAGCAACGCGGTGGT+CGG | + | chr1.4:68851255-68851274 | MS.gene21034:CDS | 50.0% | |
| TGCTTCTTCCTCCTGTCCTA+TGG | + | chr1.4:68851361-68851380 | MS.gene21034:CDS | 50.0% | |
| AGATGGTGCAGCCATAGGAC+AGG | - | chr1.4:68851375-68851394 | None:intergenic | 55.0% | |
| TGGTGCAGCCATAGGACAGG+AGG | - | chr1.4:68851372-68851391 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 68851223 | 68851396 | 68851223 | ID=MS.gene21034 |
| chr1.4 | mRNA | 68851223 | 68851396 | 68851223 | ID=MS.gene21034.t1;Parent=MS.gene21034 |
| chr1.4 | exon | 68851223 | 68851396 | 68851223 | ID=MS.gene21034.t1.exon1;Parent=MS.gene21034.t1 |
| chr1.4 | CDS | 68851223 | 68851396 | 68851223 | ID=cds.MS.gene21034.t1;Parent=MS.gene21034.t1 |
| Gene Sequence |
| Protein sequence |
