Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21189.t1 | XP_003591234.1 | 100 | 80 | 0 | 0 | 1 | 80 | 254 | 333 | 6.70E-36 | 159.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21189.t1 | Q9LZ98 | 68.8 | 80 | 19 | 2 | 1 | 79 | 254 | 328 | 8.0e-22 | 104.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21189.t1 | G7IAA4 | 100.0 | 80 | 0 | 0 | 1 | 80 | 254 | 333 | 4.8e-36 | 159.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051452 | MS.gene21189 | 0.81449 | 1.50E-51 | -1.69E-46 |
| MS.gene056787 | MS.gene21189 | 0.802743 | 4.85E-49 | -1.69E-46 |
| MS.gene056849 | MS.gene21189 | 0.819375 | 1.20E-52 | -1.69E-46 |
| MS.gene05761 | MS.gene21189 | 0.812789 | 3.55E-51 | -1.69E-46 |
| MS.gene06010 | MS.gene21189 | 0.811076 | 8.39E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21189.t1 | MTR_1g084720 | 100.000 | 80 | 0 | 0 | 1 | 80 | 254 | 333 | 4.31e-51 | 162 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21189.t1 | AT5G02270 | 71.250 | 80 | 17 | 3 | 1 | 79 | 280 | 354 | 1.19e-18 | 78.2 |
| MS.gene21189.t1 | AT5G02270 | 71.250 | 80 | 17 | 3 | 1 | 79 | 254 | 328 | 1.26e-18 | 77.8 |
Find 27 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAACTTGCTTATCAAATTC+AGG | 0.268450 | 1.4:+68669271 | None:intergenic |
| GAATTTGATAAGCAAGTTGA+TGG | 0.381823 | 1.4:-68669268 | MS.gene21189:CDS |
| TGCAGAGAACAGTAGAAGTT+TGG | 0.392482 | 1.4:-68669354 | None:intergenic |
| ATAGAAGACAAAGGAAAGAA+AGG | 0.411481 | 1.4:-68669309 | MS.gene21189:CDS |
| TCAAAACTCGGTTTGAGCTT+AGG | 0.411975 | 1.4:+68669137 | None:intergenic |
| CGGTTACATTCCACCATTGC+CGG | 0.435159 | 1.4:-68669175 | MS.gene21189:CDS |
| CCGGTTCGAGTTACTAATAA+TGG | 0.435246 | 1.4:-68669211 | MS.gene21189:CDS |
| ACTAATAATGGCTGGGCCGC+AGG | 0.461635 | 1.4:-68669199 | MS.gene21189:CDS |
| TTCGAGTTACTAATAATGGC+TGG | 0.463711 | 1.4:-68669207 | MS.gene21189:CDS |
| AGGGGATCCTGCACGAGCTC+CGG | 0.470810 | 1.4:-68669230 | MS.gene21189:CDS |
| GTTGATGGAAGCCGTGTAGT+TGG | 0.483883 | 1.4:-68669253 | MS.gene21189:CDS |
| CCATTATTAGTAACTCGAAC+CGG | 0.503384 | 1.4:+68669211 | None:intergenic |
| ACTCGAACCGGAGCTCGTGC+AGG | 0.533231 | 1.4:+68669223 | None:intergenic |
| AGGAAAGAAAGGAAGGCTGC+TGG | 0.552158 | 1.4:-68669298 | MS.gene21189:CDS |
| GATGGAAGCCGTGTAGTTGG+AGG | 0.557443 | 1.4:-68669250 | MS.gene21189:CDS |
| AAGACAAAGGAAAGAAAGGA+AGG | 0.565520 | 1.4:-68669305 | MS.gene21189:CDS |
| GAACAGTAGAAGTTTGGCTA+CGG | 0.570397 | 1.4:-68669348 | MS.gene21189:CDS |
| ATGGAAGCCGTGTAGTTGGA+GGG | 0.572562 | 1.4:-68669249 | MS.gene21189:CDS |
| AAACTCGGTTTGAGCTTAGG+AGG | 0.579078 | 1.4:+68669140 | None:intergenic |
| TCGAGTTACTAATAATGGCT+GGG | 0.593054 | 1.4:-68669206 | MS.gene21189:CDS |
| AGTAGAAGTTTGGCTACGGA+AGG | 0.617342 | 1.4:-68669344 | MS.gene21189:CDS |
| ATAATGGCTGGGCCGCAGGT+CGG | 0.623795 | 1.4:-68669195 | MS.gene21189:CDS |
| CTTGCTTATCAAATTCAGGA+AGG | 0.634035 | 1.4:+68669275 | None:intergenic |
| CAGGATCCCCTCCAACTACA+CGG | 0.662648 | 1.4:+68669242 | None:intergenic |
| TGGAAGCCGTGTAGTTGGAG+GGG | 0.669936 | 1.4:-68669248 | MS.gene21189:CDS |
| GGTGGAATGTAACCGACCTG+CGG | 0.673773 | 1.4:+68669183 | None:intergenic |
| GAGATGAAGATAGAAGACAA+AGG | 0.719714 | 1.4:-68669318 | MS.gene21189:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAGAAGACAAAGGAAAGAA+AGG | - | chr1.4:68669169-68669188 | MS.gene21189:CDS | 30.0% | |
| ! | GAATTTGATAAGCAAGTTGA+TGG | - | chr1.4:68669210-68669229 | MS.gene21189:CDS | 30.0% |
| ! | TCAACTTGCTTATCAAATTC+AGG | + | chr1.4:68669210-68669229 | None:intergenic | 30.0% |
| AAGACAAAGGAAAGAAAGGA+AGG | - | chr1.4:68669173-68669192 | MS.gene21189:CDS | 35.0% | |
| CCATTATTAGTAACTCGAAC+CGG | + | chr1.4:68669270-68669289 | None:intergenic | 35.0% | |
| CTTGCTTATCAAATTCAGGA+AGG | + | chr1.4:68669206-68669225 | None:intergenic | 35.0% | |
| GAGATGAAGATAGAAGACAA+AGG | - | chr1.4:68669160-68669179 | MS.gene21189:CDS | 35.0% | |
| TCGAGTTACTAATAATGGCT+GGG | - | chr1.4:68669272-68669291 | MS.gene21189:CDS | 35.0% | |
| ! | TTCGAGTTACTAATAATGGC+TGG | - | chr1.4:68669271-68669290 | MS.gene21189:CDS | 35.0% |
| GAACAGTAGAAGTTTGGCTA+CGG | - | chr1.4:68669130-68669149 | MS.gene21189:CDS | 40.0% | |
| ! | CCGGTTCGAGTTACTAATAA+TGG | - | chr1.4:68669267-68669286 | MS.gene21189:CDS | 40.0% |
| ! | TAAGCTCAAACCGAGTTTTG+AGG | - | chr1.4:68669343-68669362 | MS.gene21189:CDS | 40.0% |
| ! | TAGGAGGAAGTTTTCTTCAC+CGG | + | chr1.4:68669325-68669344 | None:intergenic | 40.0% |
| ! | TCAAAACTCGGTTTGAGCTT+AGG | + | chr1.4:68669344-68669363 | None:intergenic | 40.0% |
| !! | AAGCTCAAACCGAGTTTTGA+GGG | - | chr1.4:68669344-68669363 | MS.gene21189:CDS | 40.0% |
| AGTAGAAGTTTGGCTACGGA+AGG | - | chr1.4:68669134-68669153 | MS.gene21189:CDS | 45.0% | |
| ! | AAACTCGGTTTGAGCTTAGG+AGG | + | chr1.4:68669341-68669360 | None:intergenic | 45.0% |
| ! | GAAGTTTTCTTCACCGGCAA+TGG | + | chr1.4:68669319-68669338 | None:intergenic | 45.0% |
| !!! | AGCTCAAACCGAGTTTTGAG+GGG | - | chr1.4:68669345-68669364 | MS.gene21189:CDS | 45.0% |
| AGGAAAGAAAGGAAGGCTGC+TGG | - | chr1.4:68669180-68669199 | MS.gene21189:CDS | 50.0% | |
| ATGGAAGCCGTGTAGTTGGA+GGG | - | chr1.4:68669229-68669248 | MS.gene21189:CDS | 50.0% | |
| CGGTTACATTCCACCATTGC+CGG | - | chr1.4:68669303-68669322 | MS.gene21189:CDS | 50.0% | |
| ! | GTTGATGGAAGCCGTGTAGT+TGG | - | chr1.4:68669225-68669244 | MS.gene21189:CDS | 50.0% |
| ! | GTTTTCTTCACCGGCAATGG+TGG | + | chr1.4:68669316-68669335 | None:intergenic | 50.0% |
| !!! | GCTCAAACCGAGTTTTGAGG+GGG | - | chr1.4:68669346-68669365 | MS.gene21189:CDS | 50.0% |
| ACTAATAATGGCTGGGCCGC+AGG | - | chr1.4:68669279-68669298 | MS.gene21189:CDS | 55.0% | |
| CAGGATCCCCTCCAACTACA+CGG | + | chr1.4:68669239-68669258 | None:intergenic | 55.0% | |
| GATGGAAGCCGTGTAGTTGG+AGG | - | chr1.4:68669228-68669247 | MS.gene21189:CDS | 55.0% | |
| GGTGGAATGTAACCGACCTG+CGG | + | chr1.4:68669298-68669317 | None:intergenic | 55.0% | |
| TGGAAGCCGTGTAGTTGGAG+GGG | - | chr1.4:68669230-68669249 | MS.gene21189:CDS | 55.0% | |
| ATAATGGCTGGGCCGCAGGT+CGG | - | chr1.4:68669283-68669302 | MS.gene21189:CDS | 60.0% | |
| ACTCGAACCGGAGCTCGTGC+AGG | + | chr1.4:68669258-68669277 | None:intergenic | 65.0% | |
| AGGGGATCCTGCACGAGCTC+CGG | - | chr1.4:68669248-68669267 | MS.gene21189:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 68669129 | 68669371 | 68669129 | ID=MS.gene21189 |
| chr1.4 | mRNA | 68669129 | 68669371 | 68669129 | ID=MS.gene21189.t1;Parent=MS.gene21189 |
| chr1.4 | exon | 68669129 | 68669371 | 68669129 | ID=MS.gene21189.t1.exon1;Parent=MS.gene21189.t1 |
| chr1.4 | CDS | 68669129 | 68669371 | 68669129 | ID=cds.MS.gene21189.t1;Parent=MS.gene21189.t1 |
| Gene Sequence |
| Protein sequence |
