AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056787


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056787.t1 MTR_8g030830 96.066 305 12 0 1 305 1 305 0.0 583
MS.gene056787.t1 MTR_8g446960 96.066 305 12 0 1 305 1 305 0.0 562
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056787.t1 AT1G13870 70.627 303 84 4 1 303 1 298 6.99e-157 440

Find 60 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATTCTTTGAATAACATTA+AGG 0.212951 8.4:+66363185 MS.gene056787:CDS
CGATGCAATATTTGAAGATT+TGG 0.217452 8.4:+66363341 MS.gene056787:CDS
AGAATTTAAGAGGAGTTCTT+AGG 0.298956 8.4:+66363118 MS.gene056787:CDS
CCAGCTAATGGCATTTCTAT+AGG 0.311339 8.4:+66363657 MS.gene056787:CDS
TGGTGTTTGGCTCGTGCTGC+AGG 0.319369 8.4:+66363225 MS.gene056787:CDS
TCCCATCTATTTCTTCTCTC+TGG 0.324920 8.4:-66363381 None:intergenic
CCGATATGAGCTATGGTGTT+TGG 0.374786 8.4:+66363212 MS.gene056787:CDS
TGACGATTCTTTGAGAGCTT+CGG 0.376709 8.4:-66363010 None:intergenic
GGCTAGACTTAGCGCAGCTT+TGG 0.378208 8.4:-66362989 None:intergenic
CAGCTAATGGCATTTCTATA+GGG 0.379509 8.4:+66363658 MS.gene056787:CDS
AGGATGATGGTTGTAGAAAA+TGG 0.390890 8.4:+66363280 MS.gene056787:CDS
GATTCTTTGAATAACATTAA+GGG 0.396934 8.4:+66363186 MS.gene056787:CDS
AACCAGAGAGAAGAAATAGA+TGG 0.404380 8.4:+66363379 MS.gene056787:CDS
CTTGGTATGCTACCAGCTAA+TGG 0.416633 8.4:+66363645 MS.gene056787:CDS
TTCTTAGGTCAGAAGTTGAT+AGG 0.430462 8.4:+66363133 MS.gene056787:CDS
TACTGTGATGTTGAGGATGA+TGG 0.439873 8.4:+66363267 MS.gene056787:CDS
GAAGCGCAATCTCGTGATCT+TGG 0.451998 8.4:+66363627 MS.gene056787:CDS
ATCAATCTTTCGAGATCAGT+TGG 0.454210 8.4:+66363696 MS.gene056787:CDS
GTGTTGCTTTATCCAGCTCA+TGG 0.454264 8.4:-66363581 None:intergenic
ACTGATCTCGAAAGATTGAT+AGG 0.457284 8.4:-66363693 None:intergenic
CCATCAGAGAAGAATTTAAG+AGG 0.458052 8.4:+66363108 MS.gene056787:CDS
GATTACTTGAACAGAGAACT+TGG 0.460253 8.4:+66363831 MS.gene056787:CDS
TGCAAATTCTCTCCATGAGC+TGG 0.472289 8.4:+66363569 MS.gene056787:CDS
GAAGTTTGAGTCGCAATAGT+TGG 0.474380 8.4:-66363534 None:intergenic
GAGACCTCCTCCTTCTGATT+CGG 0.480952 8.4:+66363785 MS.gene056787:CDS
TTCGTAGTTTCAAATGGCAC+TGG 0.481368 8.4:+66362933 None:intergenic
CCTCTTAAATTCTTCTCTGA+TGG 0.488428 8.4:-66363108 None:intergenic
AAGCTTGTTTCCATCTTGAT+CGG 0.496584 8.4:+66363064 MS.gene056787:CDS
CGTCGCAACCTACGAAGCTC+TGG 0.498466 8.4:-66363723 None:intergenic
CAATATTTGAAGATTTGGTA+AGG 0.503933 8.4:+66363346 MS.gene056787:CDS
CCTATAGAAATGCCATTAGC+TGG 0.505528 8.4:-66363657 None:intergenic
TCAATCTTTCGAGATCAGTT+GGG 0.511829 8.4:+66363697 MS.gene056787:CDS
CGTACGTTCATGAAATTGAC+AGG 0.515117 8.4:+66363744 MS.gene056787:CDS
AACTTTGATTCCGATCAAGA+TGG 0.519631 8.4:-66363074 None:intergenic
TTTGTTTGAGTTGAAATCAT+CGG 0.521912 8.4:+66363410 MS.gene056787:CDS
CCAAACACCATAGCTCATAT+CGG 0.531120 8.4:-66363212 None:intergenic
TTGGGTTGCCAGAGCTTCGT+AGG 0.533470 8.4:+66363715 MS.gene056787:CDS
AATCAAGAGCGAAGGGAGAA+AGG 0.534973 8.4:+66363303 MS.gene056787:CDS
ACCAGAGAGAAGAAATAGAT+GGG 0.553983 8.4:+66363380 MS.gene056787:CDS
TGTTGTTTACTGTGATGTTG+AGG 0.554593 8.4:+66363260 MS.gene056787:CDS
TTGCAGTATCTTCACATCGC+GGG 0.554850 8.4:-66363511 None:intergenic
GGTGTTTGGCTCGTGCTGCA+GGG 0.562065 8.4:+66363226 MS.gene056787:CDS
GATGATTTCAACTCAAACAA+AGG 0.581112 8.4:-66363408 None:intergenic
TATTTGAAGATTTGGTAAGG+AGG 0.589953 8.4:+66363349 MS.gene056787:CDS
AGGGTTACCGATATGAGCTA+TGG 0.591762 8.4:+66363205 MS.gene056787:CDS
CAGGGCAAACAAGTTTGAGT+GGG 0.594880 8.4:+66363763 MS.gene056787:CDS
GAAAATGGAATCAAGAGCGA+AGG 0.596859 8.4:+66363295 MS.gene056787:CDS
ACAGGGCAAACAAGTTTGAG+TGG 0.597798 8.4:+66363762 MS.gene056787:CDS
TTAGCACTATCCGAATCAGA+AGG 0.613709 8.4:-66363795 None:intergenic
AAAATGGAATCAAGAGCGAA+GGG 0.624157 8.4:+66363296 MS.gene056787:CDS
GACAGCATCATCTACAACGG+AGG 0.629029 8.4:-66363457 None:intergenic
ATGGCACTGGTTGTAATATG+TGG 0.645253 8.4:+66362946 MS.gene056787:CDS
CTATCCGAATCAGAAGGAGG+AGG 0.657212 8.4:-66363789 None:intergenic
GATCTCGAAAGATTGATAGG+AGG 0.664316 8.4:-66363690 None:intergenic
GCACTATCCGAATCAGAAGG+AGG 0.671033 8.4:-66363792 None:intergenic
GCTGGATAAAGCAACACAAG+AGG 0.673279 8.4:+66363587 MS.gene056787:CDS
ATATGTGGTCAACCATGCAG+TGG 0.681649 8.4:+66362961 MS.gene056787:CDS
GTTGCAGTATCTTCACATCG+CGG 0.688808 8.4:-66363512 None:intergenic
GTACGTTCATGAAATTGACA+GGG 0.697301 8.4:+66363745 MS.gene056787:CDS
AGAGACAGCATCATCTACAA+CGG 0.703655 8.4:-66363460 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTCTTTGAATAACATTAA+GGG + chr8.4:66363186-66363205 MS.gene056787:CDS 20.0%
!! TGATTCTTTGAATAACATTA+AGG + chr8.4:66363185-66363204 MS.gene056787:CDS 20.0%
!! CAATATTTGAAGATTTGGTA+AGG + chr8.4:66363346-66363365 MS.gene056787:CDS 25.0%
!!! TTTGTTTGAGTTGAAATCAT+CGG + chr8.4:66363410-66363429 MS.gene056787:CDS 25.0%
AGAATTTAAGAGGAGTTCTT+AGG + chr8.4:66363118-66363137 MS.gene056787:CDS 30.0%
CGATGCAATATTTGAAGATT+TGG + chr8.4:66363341-66363360 MS.gene056787:CDS 30.0%
CTCTTACATAACCAAAAAAG+TGG + chr8.4:66363479-66363498 MS.gene056787:CDS 30.0%
GATGATTTCAACTCAAACAA+AGG - chr8.4:66363411-66363430 None:intergenic 30.0%
! TATTTGAAGATTTGGTAAGG+AGG + chr8.4:66363349-66363368 MS.gene056787:CDS 30.0%
AAAATGGAATCAAGAGCGAA+GGG + chr8.4:66363296-66363315 MS.gene056787:CDS 35.0%
AACCAGAGAGAAGAAATAGA+TGG + chr8.4:66363379-66363398 MS.gene056787:CDS 35.0%
AACTTTGATTCCGATCAAGA+TGG - chr8.4:66363077-66363096 None:intergenic 35.0%
ACCAGAGAGAAGAAATAGAT+GGG + chr8.4:66363380-66363399 MS.gene056787:CDS 35.0%
ACTGATCTCGAAAGATTGAT+AGG - chr8.4:66363696-66363715 None:intergenic 35.0%
ATCAATCTTTCGAGATCAGT+TGG + chr8.4:66363696-66363715 MS.gene056787:CDS 35.0%
CAGCTAATGGCATTTCTATA+GGG + chr8.4:66363658-66363677 MS.gene056787:CDS 35.0%
CCATCAGAGAAGAATTTAAG+AGG + chr8.4:66363108-66363127 MS.gene056787:CDS 35.0%
CCTCTTAAATTCTTCTCTGA+TGG - chr8.4:66363111-66363130 None:intergenic 35.0%
GATTACTTGAACAGAGAACT+TGG + chr8.4:66363831-66363850 MS.gene056787:CDS 35.0%
GTACGTTCATGAAATTGACA+GGG + chr8.4:66363745-66363764 MS.gene056787:CDS 35.0%
TCAATCTTTCGAGATCAGTT+GGG + chr8.4:66363697-66363716 MS.gene056787:CDS 35.0%
! AAGCTTGTTTCCATCTTGAT+CGG + chr8.4:66363064-66363083 MS.gene056787:CDS 35.0%
! AGGATGATGGTTGTAGAAAA+TGG + chr8.4:66363280-66363299 MS.gene056787:CDS 35.0%
! GACTCAAACTTCACGTTTTT+CGG + chr8.4:66363545-66363564 MS.gene056787:CDS 35.0%
! TGTTGTTTACTGTGATGTTG+AGG + chr8.4:66363260-66363279 MS.gene056787:CDS 35.0%
! TTCTTAGGTCAGAAGTTGAT+AGG + chr8.4:66363133-66363152 MS.gene056787:CDS 35.0%
!!! GGTTTTTGAGTCCACTTTTT+TGG - chr8.4:66363493-66363512 None:intergenic 35.0%
AGAGACAGCATCATCTACAA+CGG - chr8.4:66363463-66363482 None:intergenic 40.0%
CCAAACACCATAGCTCATAT+CGG - chr8.4:66363215-66363234 None:intergenic 40.0%
CCAGCTAATGGCATTTCTAT+AGG + chr8.4:66363657-66363676 MS.gene056787:CDS 40.0%
CCTATAGAAATGCCATTAGC+TGG - chr8.4:66363660-66363679 None:intergenic 40.0%
CGTACGTTCATGAAATTGAC+AGG + chr8.4:66363744-66363763 MS.gene056787:CDS 40.0%
GAAAATGGAATCAAGAGCGA+AGG + chr8.4:66363295-66363314 MS.gene056787:CDS 40.0%
GAAGTTTGAGTCGCAATAGT+TGG - chr8.4:66363537-66363556 None:intergenic 40.0%
GATCTCGAAAGATTGATAGG+AGG - chr8.4:66363693-66363712 None:intergenic 40.0%
TACTGTGATGTTGAGGATGA+TGG + chr8.4:66363267-66363286 MS.gene056787:CDS 40.0%
TTAGCACTATCCGAATCAGA+AGG - chr8.4:66363798-66363817 None:intergenic 40.0%
! TCCCATCTATTTCTTCTCTC+TGG - chr8.4:66363384-66363403 None:intergenic 40.0%
! TGACGATTCTTTGAGAGCTT+CGG - chr8.4:66363013-66363032 None:intergenic 40.0%
!! ATGGCACTGGTTGTAATATG+TGG + chr8.4:66362946-66362965 MS.gene056787:CDS 40.0%
AATCAAGAGCGAAGGGAGAA+AGG + chr8.4:66363303-66363322 MS.gene056787:CDS 45.0%
ACAGGGCAAACAAGTTTGAG+TGG + chr8.4:66363762-66363781 MS.gene056787:CDS 45.0%
AGGGTTACCGATATGAGCTA+TGG + chr8.4:66363205-66363224 MS.gene056787:CDS 45.0%
ATATGTGGTCAACCATGCAG+TGG + chr8.4:66362961-66362980 MS.gene056787:CDS 45.0%
CAGGGCAAACAAGTTTGAGT+GGG + chr8.4:66363763-66363782 MS.gene056787:CDS 45.0%
CCGATATGAGCTATGGTGTT+TGG + chr8.4:66363212-66363231 MS.gene056787:CDS 45.0%
CTTGGTATGCTACCAGCTAA+TGG + chr8.4:66363645-66363664 MS.gene056787:CDS 45.0%
GCTGGATAAAGCAACACAAG+AGG + chr8.4:66363587-66363606 MS.gene056787:CDS 45.0%
GTTGCAGTATCTTCACATCG+CGG - chr8.4:66363515-66363534 None:intergenic 45.0%
TGCAAATTCTCTCCATGAGC+TGG + chr8.4:66363569-66363588 MS.gene056787:CDS 45.0%
TTGCAGTATCTTCACATCGC+GGG - chr8.4:66363514-66363533 None:intergenic 45.0%
!! GCTTTGGATTTTCCACTGCA+TGG - chr8.4:66362976-66362995 None:intergenic 45.0%
!! GTGTTGCTTTATCCAGCTCA+TGG - chr8.4:66363584-66363603 None:intergenic 45.0%
CTATCCGAATCAGAAGGAGG+AGG - chr8.4:66363792-66363811 None:intergenic 50.0%
GAAGCGCAATCTCGTGATCT+TGG + chr8.4:66363627-66363646 MS.gene056787:CDS 50.0%
GACAGCATCATCTACAACGG+AGG - chr8.4:66363460-66363479 None:intergenic 50.0%
GAGACCTCCTCCTTCTGATT+CGG + chr8.4:66363785-66363804 MS.gene056787:CDS 50.0%
GCACTATCCGAATCAGAAGG+AGG - chr8.4:66363795-66363814 None:intergenic 50.0%
GGCTAGACTTAGCGCAGCTT+TGG - chr8.4:66362992-66363011 None:intergenic 55.0%
! TTGGGTTGCCAGAGCTTCGT+AGG + chr8.4:66363715-66363734 MS.gene056787:CDS 55.0%
CGTCGCAACCTACGAAGCTC+TGG - chr8.4:66363726-66363745 None:intergenic 60.0%
! GGTGTTTGGCTCGTGCTGCA+GGG + chr8.4:66363226-66363245 MS.gene056787:CDS 60.0%
! TGGTGTTTGGCTCGTGCTGC+AGG + chr8.4:66363225-66363244 MS.gene056787:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 66362946 66363863 66362946 ID=MS.gene056787
chr8.4 mRNA 66362946 66363863 66362946 ID=MS.gene056787.t1;Parent=MS.gene056787
chr8.4 exon 66362946 66363863 66362946 ID=MS.gene056787.t1.exon1;Parent=MS.gene056787.t1
chr8.4 CDS 66362946 66363863 66362946 ID=cds.MS.gene056787.t1;Parent=MS.gene056787.t1
Gene Sequence

>MS.gene056787

ATGGCACTGGTTGTAATATGTGGTCAACCATGCAGTGGAAAATCCAAAGCTGCGCTAAGTCTAGCCGAAGCTCTCAAAGAATCGTCAGAATTCAAACATCAAGTGAGAATCATAGATGAAGCTTGTTTCCATCTTGATCGGAATCAAAGTTATGCAAATATGCCATCAGAGAAGAATTTAAGAGGAGTTCTTAGGTCAGAAGTTGATAGGTCTCTGTCAAAAGATACTGTTATTATCGTTGATTCTTTGAATAACATTAAGGGTTACCGATATGAGCTATGGTGTTTGGCTCGTGCTGCAGGGATTAGATACTGTGTTGTTTACTGTGATGTTGAGGATGATGGTTGTAGAAAATGGAATCAAGAGCGAAGGGAGAAAGGTGAAGATAATTATGACGATGCAATATTTGAAGATTTGGTAAGGAGGTTTGAGAAACCAGAGAGAAGAAATAGATGGGATTCACCTTTGTTTGAGTTGAAATCATCGGCATCGTCTTCTTCGTCTGATTCTGCCTCCGTTGTAGATGATGCTGTCTCTTACATAACCAAAAAAGTGGACTCAAAAACCCGCGATGTGAAGATACTGCAACCAACTATTGCGACTCAAACTTCACGTTTTTCGGATGCAAATTCTCTCCATGAGCTGGATAAAGCAACACAAGAGGTTACTAATGCTATAGCAGAAGCGCAATCTCGTGATCTTGGTATGCTACCAGCTAATGGCATTTCTATAGGGAAAGACTTGCCTCCTATCAATCTTTCGAGATCAGTTGGGTTGCCAGAGCTTCGTAGGTTGCGACGTACGTTCATGAAATTGACAGGGCAAACAAGTTTGAGTGGGAGACCTCCTCCTTCTGATTCGGATAGTGCTAAAAGAATGTTCATTGATTACTTGAACAGAGAACTTGGAACTTCATGA

Protein sequence

>MS.gene056787.t1

MALVVICGQPCSGKSKAALSLAEALKESSEFKHQVRIIDEACFHLDRNQSYANMPSEKNLRGVLRSEVDRSLSKDTVIIVDSLNNIKGYRYELWCLARAAGIRYCVVYCDVEDDGCRKWNQERREKGEDNYDDAIFEDLVRRFEKPERRNRWDSPLFELKSSASSSSSDSASVVDDAVSYITKKVDSKTRDVKILQPTIATQTSRFSDANSLHELDKATQEVTNAIAEAQSRDLGMLPANGISIGKDLPPINLSRSVGLPELRRLRRTFMKLTGQTSLSGRPPPSDSDSAKRMFIDYLNRELGTS