AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21256

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21256.t1 MTR_5g067350 97.118 347 10 0 1 347 1 347 0.0 691
MS.gene21256.t1 MTR_5g067350 95.956 272 11 0 1 272 1 272 0.0 528
MS.gene21256.t1 MTR_8g067530 63.240 321 103 9 32 347 46 356 6.84e-132 380
MS.gene21256.t1 MTR_5g030710 62.577 326 100 9 33 347 21 335 1.31e-127 369
MS.gene21256.t1 MTR_2g101500 49.284 349 123 11 29 347 2 326 1.59e-91 277
MS.gene21256.t1 MTR_6g488150 52.881 295 88 9 57 347 7 254 4.24e-87 263
MS.gene21256.t1 MTR_8g103030 47.273 330 104 9 50 347 2 293 9.73e-84 256
MS.gene21256.t1 MTR_1g080860 65.854 164 51 3 188 346 89 252 1.80e-69 218
MS.gene21256.t1 MTR_7g096090 67.089 158 50 2 191 347 94 250 7.79e-69 216
MS.gene21256.t1 MTR_1g070520 65.217 161 51 4 191 346 84 244 2.58e-67 212
MS.gene21256.t1 MTR_1g093240 62.264 159 58 2 191 347 78 236 1.80e-65 207
MS.gene21256.t1 MTR_1g080860 64.286 154 50 3 188 336 89 242 8.59e-63 200
MS.gene21256.t1 MTR_1g093240 61.905 147 54 2 191 335 78 224 7.49e-59 190
MS.gene21256.t1 MTR_1g070830 55.357 168 47 5 175 336 57 202 4.36e-50 166
MS.gene21256.t1 MTR_1g040675 51.515 165 56 4 172 336 43 183 1.41e-47 159
MS.gene21256.t1 MTR_1g093240 60.630 127 48 2 191 315 78 204 1.90e-47 159
MS.gene21256.t1 MTR_1g076270 52.229 157 48 5 180 335 48 178 9.77e-47 157
MS.gene21256.t1 MTR_1g093350 52.229 157 48 5 180 335 48 178 9.77e-47 157
MS.gene21256.t1 MTR_6g488150 44.700 217 72 8 57 272 7 176 1.75e-40 141
MS.gene21256.t1 MTR_1g040675 51.064 141 45 4 175 315 46 162 1.12e-37 132
MS.gene21256.t1 MTR_1g069495 44.022 184 68 5 195 343 81 264 6.75e-37 134
MS.gene21256.t1 MTR_4g011880 49.324 148 46 4 194 339 52 172 3.00e-35 127
MS.gene21256.t1 MTR_2g100780 40.217 184 78 6 182 338 85 263 1.46e-31 120
MS.gene21256.t1 MTR_8g014520 41.279 172 83 4 178 331 89 260 1.56e-31 120
MS.gene21256.t1 MTR_8g014520 41.279 172 83 4 178 331 89 260 1.58e-31 120
MS.gene21256.t1 MTR_2g100780 40.571 175 74 5 182 331 85 254 2.52e-31 119
MS.gene21256.t1 MTR_2g102490 54.902 102 37 3 232 330 88 183 2.87e-31 117
MS.gene21256.t1 MTR_4g124300 34.437 151 69 6 186 330 56 182 3.70e-19 84.3
MS.gene21256.t1 MTR_1g085750 40.741 108 48 3 230 335 83 176 2.64e-17 79.0
MS.gene21256.t1 MTR_5g057080 55.128 78 12 1 114 191 22 76 6.13e-17 75.9
MS.gene21256.t1 MTR_3g106850 31.469 143 72 5 196 332 88 210 2.64e-16 77.0
MS.gene21256.t1 MTR_5g043705 70.000 50 15 0 154 203 17 66 1.34e-15 71.6
MS.gene21256.t1 MTR_7g110790 38.384 99 50 2 230 328 68 155 1.35e-15 73.9
MS.gene21256.t1 MTR_1g109510 31.690 142 80 3 187 328 44 168 2.42e-15 73.6
MS.gene21256.t1 MTR_4g115075 31.159 138 66 6 197 329 93 206 2.01e-14 71.6
MS.gene21256.t1 MTR_1g085750 40.909 88 37 2 230 315 83 157 6.81e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21256.t1 AT2G22670 56.250 336 108 12 20 346 13 318 1.12e-109 323
MS.gene21256.t1 AT2G22670 56.250 336 108 12 20 346 13 318 1.12e-109 323
MS.gene21256.t1 AT2G22670 56.250 336 108 12 20 346 30 335 2.19e-109 323
MS.gene21256.t1 AT5G65670 57.188 320 116 9 32 346 32 335 1.57e-108 320
MS.gene21256.t1 AT5G65670 56.832 322 116 10 32 346 32 337 1.16e-106 316
MS.gene21256.t1 AT2G22670 55.655 336 108 13 20 346 13 316 2.09e-106 314
MS.gene21256.t1 AT2G22670 55.655 336 108 13 20 346 13 316 2.09e-106 314
MS.gene21256.t1 AT4G29080 48.160 326 130 7 33 347 8 305 4.36e-87 265
MS.gene21256.t1 AT4G14550 45.070 284 99 8 65 347 1 228 6.04e-66 208
MS.gene21256.t1 AT1G04250 42.160 287 96 6 65 346 7 228 1.19e-64 204
MS.gene21256.t1 AT3G04730 43.098 297 91 8 65 346 2 235 3.87e-63 201
MS.gene21256.t1 AT3G23050 43.902 287 97 9 65 342 6 237 1.59e-61 197
MS.gene21256.t1 AT4G14550 43.956 273 96 8 65 336 1 217 8.06e-60 192
MS.gene21256.t1 AT1G04240 53.548 155 54 4 182 336 50 186 3.10e-49 164
MS.gene21256.t1 AT1G04240 53.548 155 54 4 182 336 150 286 2.60e-48 164
MS.gene21256.t1 AT4G14550 42.231 251 88 8 65 314 1 195 1.88e-47 159
MS.gene21256.t1 AT5G43700 50.649 154 56 3 183 336 49 182 3.27e-45 153
MS.gene21256.t1 AT3G23050 41.154 260 89 9 65 315 6 210 2.32e-44 152
MS.gene21256.t1 AT3G23030 48.980 147 46 2 189 335 53 170 4.40e-41 142
MS.gene21256.t1 AT3G15540 49.359 156 54 5 190 342 64 197 2.24e-40 141
MS.gene21256.t1 AT3G23030 48.980 147 46 2 189 335 140 257 2.48e-40 143
MS.gene21256.t1 AT2G33310 44.624 186 67 6 182 341 62 237 1.55e-38 137
MS.gene21256.t1 AT1G52830 45.333 150 57 4 187 336 61 185 1.81e-38 135
MS.gene21256.t1 AT2G33310 44.624 186 66 7 182 341 62 236 6.11e-37 133
MS.gene21256.t1 AT2G33310 44.624 186 66 7 182 341 62 236 6.11e-37 133
MS.gene21256.t1 AT4G28640 46.914 162 66 4 192 336 84 242 1.99e-36 132
MS.gene21256.t1 AT4G28640 46.875 160 65 4 192 334 84 240 3.35e-36 132
MS.gene21256.t1 AT1G04550 42.564 195 82 5 175 347 50 236 4.46e-36 131
MS.gene21256.t1 AT1G15580 39.665 179 72 4 158 330 11 159 1.38e-35 127
MS.gene21256.t1 AT4G28640 46.875 160 65 4 192 334 84 240 2.61e-35 130
MS.gene21256.t1 AT4G14560 49.655 145 46 3 188 332 47 164 1.63e-34 125
MS.gene21256.t1 AT1G04100 39.655 174 72 5 193 336 90 260 6.64e-31 118
MS.gene21256.t1 AT3G16500 38.547 179 74 5 185 331 78 252 4.23e-30 116
MS.gene21256.t1 AT3G16500 38.547 179 74 5 185 331 78 252 4.77e-30 116
MS.gene21256.t1 AT1G51950 38.462 169 80 5 183 331 86 250 9.50e-30 115
MS.gene21256.t1 AT5G25890 36.095 169 88 3 170 338 22 170 9.13e-27 104
MS.gene21256.t1 AT4G14550 37.799 209 70 8 65 271 1 151 1.29e-26 104
MS.gene21256.t1 AT3G17600 38.750 160 69 7 175 328 18 154 3.06e-24 97.4
MS.gene21256.t1 AT3G23050 36.744 215 73 8 65 271 6 165 2.05e-23 95.5
MS.gene21256.t1 AT2G46990 40.385 104 50 1 227 330 81 172 1.47e-19 85.1
MS.gene21256.t1 AT3G62100 39.604 101 49 1 229 329 81 169 2.53e-19 84.3
MS.gene21256.t1 AT3G62100 39.080 87 41 1 229 315 81 155 4.33e-14 69.7
MS.gene21256.t1 AT2G46990 39.080 87 41 1 229 315 83 157 7.41e-14 68.9
MS.gene21256.t1 AT1G15050 37.255 102 51 1 232 333 62 150 8.11e-14 68.6
MS.gene21256.t1 AT1G15050 37.255 102 51 1 232 333 94 182 1.74e-13 68.6
MS.gene21256.t1 AT2G01200 34.906 106 56 1 228 333 96 188 9.31e-13 66.6
MS.gene21256.t1 AT2G01200 34.906 106 56 1 228 333 96 188 9.31e-13 66.6
MS.gene21256.t1 AT4G32280 26.667 165 73 5 195 328 93 240 2.48e-12 66.6
MS.gene21256.t1 AT4G32280 32.653 98 49 2 231 328 118 198 2.65e-12 65.9

Find 64 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAGGTTGATGGAAAAT+TGG 0.178401 5.1:+52245241 MS.gene21256:CDS
GGCAACATTCTCAAGCATTT+TGG 0.180467 5.1:-52244632 None:intergenic
AAATCTGATGAATCTCTTTC+AGG 0.213261 5.1:-52244225 None:intergenic
CCCATGTAGTTGAACTCTTT+TGG 0.273911 5.1:-52244072 None:intergenic
TTTAAGATTCAAATTACTTT+TGG 0.274939 5.1:-52244163 None:intergenic
GAGCTTAGATTGGGACTTCC+AGG 0.313169 5.1:+52244192 MS.gene21256:CDS
GAAAGCAAACCAAGTCTTAA+TGG 0.318890 5.1:+52244724 MS.gene21256:CDS
TGCTTGTGGGTGATGTTCCC+TGG 0.320651 5.1:+52245615 MS.gene21256:CDS
AGCTCTGATGCCATTGGTTT+AGG 0.336962 5.1:+52246653 MS.gene21256:CDS
TAAAGCTACTGAGCTTAGAT+TGG 0.371109 5.1:+52244182 MS.gene21256:CDS
CTTAATGGTTCTGCAACTAA+TGG 0.371208 5.1:+52244739 MS.gene21256:CDS
TACCTCTGAGAAAACATTCA+TGG 0.380080 5.1:-52244370 None:intergenic
CTTTCAGGAGAAAGTGATCC+TGG 0.402526 5.1:-52244210 None:intergenic
GAAGAGGTTGATGGAAAATT+GGG 0.409009 5.1:+52245242 MS.gene21256:CDS
TGTGGCAGGGCACAGGTTGT+TGG 0.411315 5.1:+52245161 MS.gene21256:intron
AGAATATGTTATTACTTATG+AGG 0.411394 5.1:+52245574 MS.gene21256:CDS
ATCCACTGATGAACAATTAG+TGG 0.425775 5.1:-52244103 None:intergenic
AAAATGCTTGAGAATGTTGC+CGG 0.428584 5.1:+52244634 MS.gene21256:CDS
AAGCTGAGGGATCTGCTTCA+TGG 0.432732 5.1:+52245548 MS.gene21256:CDS
GGCCTCCAATAAGATCGTTT+AGG 0.457342 5.1:+52245186 MS.gene21256:CDS
AAGAACAATGAAGAGGTTGA+TGG 0.459868 5.1:+52245233 MS.gene21256:CDS
ATTGGTCTTGAGAGGCCTAA+TGG 0.460399 5.1:+52244694 MS.gene21256:CDS
CTTAACTGTTGCTATTTCAT+CGG 0.466939 5.1:-52244671 None:intergenic
TCATCACCTATTCCTAGCCT+TGG 0.471158 5.1:-52243991 None:intergenic
GCAGGGCACAGGTTGTTGGT+TGG 0.475629 5.1:+52245165 MS.gene21256:intron
GAAATAGCAACAGTTAAGAT+TGG 0.476375 5.1:+52244676 MS.gene21256:CDS
TCTCTACCAAGGCTAGGAAT+AGG 0.484039 5.1:+52243985 MS.gene21256:CDS
TTGCTTTCACCGACACCATT+AGG 0.485059 5.1:-52244709 None:intergenic
TTCCTAAACGATCTTATTGG+AGG 0.485580 5.1:-52245188 None:intergenic
GTTGATGGAAAATTGGGATC+AGG 0.490761 5.1:+52245248 MS.gene21256:CDS
ATTACTTATGAGGATAAAGA+TGG 0.497598 5.1:+52245584 MS.gene21256:CDS
AAAGCTACTGAGCTTAGATT+GGG 0.502103 5.1:+52244183 MS.gene21256:CDS
ACCAAAAGAGTTCAACTACA+TGG 0.503124 5.1:+52244071 MS.gene21256:CDS
GCTTGTGGGTGATGTTCCCT+GGG 0.507108 5.1:+52245616 MS.gene21256:CDS
ATGAGGATAAAGATGGTGAT+TGG 0.518002 5.1:+52245591 MS.gene21256:CDS
ATGAAGAGCTCTGATGCCAT+TGG 0.522246 5.1:+52246647 MS.gene21256:CDS
ATGTTCAGCTGTTTCACCAT+AGG 0.526626 5.1:+52245377 MS.gene21256:CDS
TTCATTGTTCTTTGAAGCAG+CGG 0.526792 5.1:-52245222 None:intergenic
AATCTTGTGTGGCAGGGCAC+AGG 0.532182 5.1:+52245154 MS.gene21256:intron
CACCCTACTTACTTGCTAGC+AGG 0.532961 5.1:-52244775 None:intergenic
GATGGTGATTGGATGCTTGT+GGG 0.535428 5.1:+52245602 MS.gene21256:CDS
TTTGTGAAGGTCAGCATGGA+TGG 0.538161 5.1:+52245281 MS.gene21256:CDS
ATCGGCTTTCACTTTCTGCC+CGG 0.540054 5.1:-52244653 None:intergenic
GGATGTTTATTGATACATGC+AGG 0.544403 5.1:+52246612 MS.gene21256:intron
TCAGGTCAATGTGAATCTCA+TGG 0.548741 5.1:+52245503 MS.gene21256:intron
CAGTTAAGATTGGTCTTGAG+AGG 0.564900 5.1:+52244686 MS.gene21256:CDS
GTTGCAGAACCATTAAGACT+TGG 0.566322 5.1:-52244733 None:intergenic
ATGAAGCAGATCCCTCAGCT+TGG 0.567500 5.1:-52245546 None:intergenic
AGATGGTGATTGGATGCTTG+TGG 0.576179 5.1:+52245601 MS.gene21256:CDS
ATCCACTAATTGTTCATCAG+TGG 0.582589 5.1:+52244101 MS.gene21256:CDS
GTGATGTCTCTACCAAGGCT+AGG 0.589347 5.1:+52243979 None:intergenic
AAATGCTTGAGAATGTTGCC+GGG 0.605417 5.1:+52244635 MS.gene21256:CDS
GGAATACTAACCTAAACCAA+TGG 0.612277 5.1:-52246663 None:intergenic
TGCTTCAAAGAACAATGAAG+AGG 0.614173 5.1:+52245226 MS.gene21256:CDS
CCAAAAGAGTTCAACTACAT+GGG 0.615578 5.1:+52244072 MS.gene21256:CDS
TGCTGAATGAAACCAAGCTG+AGG 0.622855 5.1:+52245534 MS.gene21256:CDS
GCTGAATGAAACCAAGCTGA+GGG 0.626284 5.1:+52245535 MS.gene21256:CDS
AGTACATAAACTTACTCCCA+GGG 0.630905 5.1:-52245632 None:intergenic
GATGGAAAATTGGGATCAGG+AGG 0.633939 5.1:+52245251 MS.gene21256:CDS
CTTGAGAGGCCTAATGGTGT+CGG 0.641657 5.1:+52244700 MS.gene21256:CDS
AGGAGGTGCTGTGTTTGTGA+AGG 0.651647 5.1:+52245268 MS.gene21256:CDS
TCATTGTGATGTCTCTACCA+AGG 0.666331 5.1:+52243974 None:intergenic
TGTGTTTGTGAAGGTCAGCA+TGG 0.667221 5.1:+52245277 MS.gene21256:CDS
TGTTTATTGATACATGCAGG+AGG 0.676885 5.1:+52246615 MS.gene21256:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATCAAAAATAGAAAT+TGG - chr5.1:52246814-52246833 None:intergenic 10.0%
!!! TCTTTAAAAGATTGAATTTT+TGG - chr5.1:52244138-52244157 None:intergenic 15.0%
!!! TTTAAGATTCAAATTACTTT+TGG - chr5.1:52244166-52244185 None:intergenic 15.0%
!! AAATCACAAAAATTGAAATG+CGG - chr5.1:52244968-52244987 None:intergenic 20.0%
!! AATAAAAGCTTGAATGATAA+TGG + chr5.1:52246091-52246110 MS.gene21256:intron 20.0%
!! AATGTGACTTCAAATTAAAA+TGG + chr5.1:52246172-52246191 MS.gene21256:intron 20.0%
!! ACTAAATGAATATGTTACAT+GGG + chr5.1:52246356-52246375 MS.gene21256:intron 20.0%
!! AGAATATGTTATTACTTATG+AGG + chr5.1:52245574-52245593 MS.gene21256:CDS 20.0%
!! ATGTAAGGTATATTAATAGA+AGG + chr5.1:52246024-52246043 MS.gene21256:intron 20.0%
!! TTCTATTAATATACCTTACA+TGG - chr5.1:52246025-52246044 None:intergenic 20.0%
!! TTTATATTGCAGTTAAAATG+CGG + chr5.1:52244457-52244476 MS.gene21256:intron 20.0%
!!! AAAGTAAATTAAGTTGAAGT+AGG - chr5.1:52246790-52246809 None:intergenic 20.0%
!!! CACATTTTATTATCTTATCA+CGG + chr5.1:52245854-52245873 MS.gene21256:intron 20.0%
!!! TAAAAACAATGTTACTTTGT+TGG + chr5.1:52244020-52244039 MS.gene21256:CDS 20.0%
!!! TTGATACGATTTTATTGAAA+GGG + chr5.1:52246460-52246479 MS.gene21256:intron 20.0%
!!! TTTGATACGATTTTATTGAA+AGG + chr5.1:52246459-52246478 MS.gene21256:intron 20.0%
! AAATCAAAGTGAAGTAAACA+AGG - chr5.1:52246537-52246556 None:intergenic 25.0%
! ATTACTTATGAGGATAAAGA+TGG + chr5.1:52245584-52245603 MS.gene21256:CDS 25.0%
! CACTAAATGAATATGTTACA+TGG + chr5.1:52246355-52246374 MS.gene21256:intron 25.0%
! CTAAATGAATATGTTACATG+GGG + chr5.1:52246357-52246376 MS.gene21256:intron 25.0%
! CTTAACAATGAAAATGAACT+CGG + chr5.1:52245117-52245136 MS.gene21256:intron 25.0%
! GATTGATTGTGTTAAATGAA+TGG - chr5.1:52246687-52246706 None:intergenic 25.0%
!! AAAAGATGATCATCTTTTTG+AGG - chr5.1:52244324-52244305 None:intergenic 25.0%
!! AAAGATGATCATCTTTTTGA+GGG - chr5.1:52244325-52244306 None:intergenic 25.0%
!! CACATAATTTGTACTTGAAT+TGG - chr5.1:52246869-52246888 None:intergenic 25.0%
!! TTTGAATTGGCTTAAATTGT+TGG - chr5.1:52246199-52246218 None:intergenic 25.0%
!! TTTTTCCTAAACGATCTTAT+TGG - chr5.1:52245194-52245213 None:intergenic 25.0%
!!! AAGTCTACTTTTCTCAAATA+CGG - chr5.1:52245311-52245330 None:intergenic 25.0%
!!! TACTGCAAGGTTTTAAATTT+AGG + chr5.1:52244819-52244838 MS.gene21256:intron 25.0%
!!! TGTATGATGGTTTTAAATTG+CGG + chr5.1:52244414-52244433 MS.gene21256:intron 25.0%
!!! TTGTGGTTTTAGTTTGAAAT+AGG + chr5.1:52245995-52246014 MS.gene21256:intron 25.0%
AAATCGTATCAAATTCGCTA+TGG - chr5.1:52246452-52246471 None:intergenic 30.0%
AAATCTGATGAATCTCTTTC+AGG - chr5.1:52244228-52244247 None:intergenic 30.0%
AATAGTCTTTCAACGATTTG+AGG + chr5.1:52245435-52245454 MS.gene21256:intron 30.0%
AATATATCTCAACTCTTTGC+AGG + chr5.1:52246591-52246610 MS.gene21256:intron 30.0%
ACCATTATTTCGTCTAACTA+CGG - chr5.1:52246244-52246263 None:intergenic 30.0%
AGTTGAGATATATTAGGTGT+TGG - chr5.1:52246585-52246604 None:intergenic 30.0%
ATGAGGAAAATTCTTAGAGA+GGG + chr5.1:52246483-52246502 MS.gene21256:intron 30.0%
ATGTTTGGAAATTGGAATCA+TGG - chr5.1:52246326-52246345 None:intergenic 30.0%
CAAGTACAAATTATGTGTTG+TGG + chr5.1:52246872-52246891 MS.gene21256:intron 30.0%
CAGTTGAAAAAAGTAAAAGC+AGG + chr5.1:52246916-52246935 MS.gene21256:CDS 30.0%
GAAATAGCAACAGTTAAGAT+TGG + chr5.1:52244676-52244695 MS.gene21256:CDS 30.0%
GCAAAGAGTTGAGATATATT+AGG - chr5.1:52246591-52246610 None:intergenic 30.0%
GTCTTCAATAATGCCAAAAA+TGG - chr5.1:52246060-52246079 None:intergenic 30.0%
TGAAACGCAAAATTGAAAAC+AGG - chr5.1:52246400-52246419 None:intergenic 30.0%
! ATACAAATTTTGCAGCATTG+TGG + chr5.1:52245978-52245997 MS.gene21256:intron 30.0%
! ATTCAATCGAAAGTGTATGA+TGG + chr5.1:52244401-52244420 MS.gene21256:intron 30.0%
! GAAAAAAGTGTGTGTTTGAA+TGG + chr5.1:52244042-52244061 MS.gene21256:CDS 30.0%
!! CTGTTTTAGGTAACAAAAGA+GGG + chr5.1:52244337-52244356 MS.gene21256:CDS 30.0%
!! CTTAACTGTTGCTATTTCAT+CGG - chr5.1:52244674-52244693 None:intergenic 30.0%
!!! CTTTTTTTAGAAGCTTGGTT+TGG + chr5.1:52245094-52245113 MS.gene21256:intron 30.0%
!!! TGGTTTTAGTTTGAAATAGG+TGG + chr5.1:52245998-52246017 MS.gene21256:intron 30.0%
!!! TTACTTTTTTCAACTGCTCT+GGG - chr5.1:52246912-52246931 None:intergenic 30.0%
!!! TTTACTTTTTTCAACTGCTC+TGG - chr5.1:52246913-52246932 None:intergenic 30.0%
AAAATGCTTGAGAATGTTGC+CGG + chr5.1:52244634-52244653 MS.gene21256:CDS 35.0%
AAAGCTACTGAGCTTAGATT+GGG + chr5.1:52244183-52244202 MS.gene21256:CDS 35.0%
AAGAACAATGAAGAGGTTGA+TGG + chr5.1:52245233-52245252 MS.gene21256:CDS 35.0%
AAGTACATAAACTTACTCCC+AGG - chr5.1:52245636-52245655 None:intergenic 35.0%
ACCAAAAGAGTTCAACTACA+TGG + chr5.1:52244071-52244090 MS.gene21256:CDS 35.0%
AGTACATAAACTTACTCCCA+GGG - chr5.1:52245635-52245654 None:intergenic 35.0%
ATCCACTAATTGTTCATCAG+TGG + chr5.1:52244101-52244120 MS.gene21256:CDS 35.0%
ATCCACTGATGAACAATTAG+TGG - chr5.1:52244106-52244125 None:intergenic 35.0%
ATCGCAGTTACTTACAAATG+CGG + chr5.1:52244893-52244912 MS.gene21256:intron 35.0%
ATGACTGCATGTTTGGAAAT+TGG - chr5.1:52246334-52246353 None:intergenic 35.0%
ATGAGGATAAAGATGGTGAT+TGG + chr5.1:52245591-52245610 MS.gene21256:CDS 35.0%
CCAAAAGAGTTCAACTACAT+GGG + chr5.1:52244072-52244091 MS.gene21256:CDS 35.0%
CTTGAATCACGATTGTATCA+CGG + chr5.1:52245020-52245039 MS.gene21256:intron 35.0%
GAAAGCAAACCAAGTCTTAA+TGG + chr5.1:52244724-52244743 MS.gene21256:CDS 35.0%
GATGAGGAAAATTCTTAGAG+AGG + chr5.1:52246482-52246501 MS.gene21256:intron 35.0%
GATGATTGATGAATCTTGTG+TGG + chr5.1:52245143-52245162 MS.gene21256:intron 35.0%
GCAGTACAAATAGTACAGTA+TGG - chr5.1:52246124-52246143 None:intergenic 35.0%
GCTTATGTTGATGTGATTTC+AGG + chr5.1:52245485-52245504 MS.gene21256:intron 35.0%
GGAATACTAACCTAAACCAA+TGG - chr5.1:52246666-52246685 None:intergenic 35.0%
GGATGTTTATTGATACATGC+AGG + chr5.1:52246612-52246631 MS.gene21256:intron 35.0%
TAAAGCTACTGAGCTTAGAT+TGG + chr5.1:52244182-52244201 MS.gene21256:CDS 35.0%
TGCTTCAAAGAACAATGAAG+AGG + chr5.1:52245226-52245245 MS.gene21256:CDS 35.0%
TGGGAGTAAGTTTATGTACT+TGG + chr5.1:52245635-52245654 MS.gene21256:intron 35.0%
TGTTTATTGATACATGCAGG+AGG + chr5.1:52246615-52246634 MS.gene21256:CDS 35.0%
TTAGTGTATGACTGCATGTT+TGG - chr5.1:52246341-52246360 None:intergenic 35.0%
TTCCTAAACGATCTTATTGG+AGG - chr5.1:52245191-52245210 None:intergenic 35.0%
TTTGTAAGTAACTGCGATTC+AGG - chr5.1:52244891-52244910 None:intergenic 35.0%
! CGATTTTATTGAAAGGGATG+AGG + chr5.1:52246466-52246485 MS.gene21256:intron 35.0%
! CTTAATGGTTCTGCAACTAA+TGG + chr5.1:52244739-52244758 MS.gene21256:CDS 35.0%
! CTTTTGTTACCTAAAACAGC+AGG - chr5.1:52244336-52244355 None:intergenic 35.0%
! TACCTCTGAGAAAACATTCA+TGG - chr5.1:52244373-52244392 None:intergenic 35.0%
!! GAAGAGGTTGATGGAAAATT+GGG + chr5.1:52245242-52245261 MS.gene21256:CDS 35.0%
!! GCTGTTTTAGGTAACAAAAG+AGG + chr5.1:52244336-52244355 MS.gene21256:CDS 35.0%
!! TGAAGAGGTTGATGGAAAAT+TGG + chr5.1:52245241-52245260 MS.gene21256:CDS 35.0%
!! TTCATTGTTCTTTGAAGCAG+CGG - chr5.1:52245225-52245244 None:intergenic 35.0%
!! TTTGAGGCAATTTTTGTGTC+TGG + chr5.1:52245451-52245470 MS.gene21256:intron 35.0%
!!! ACAAGCGCTAAGTTTTGAAT+TGG - chr5.1:52246212-52246231 None:intergenic 35.0%
!!! AGGTAAGAAACTGCCATTTT+TGG + chr5.1:52246044-52246063 MS.gene21256:intron 35.0%
!!! GCGAACTTTTTTTAGAAGCT+TGG + chr5.1:52245089-52245108 MS.gene21256:intron 35.0%
!!! TACTTTTTTCAACTGCTCTG+GGG - chr5.1:52246911-52246930 None:intergenic 35.0%
AAATGCTTGAGAATGTTGCC+GGG + chr5.1:52244635-52244654 MS.gene21256:CDS 40.0%
AACCTATTCCTACCTACCTA+TGG - chr5.1:52245396-52245415 None:intergenic 40.0%
ATTGATGAATCTTGTGTGGC+AGG + chr5.1:52245147-52245166 MS.gene21256:intron 40.0%
CCCTCTGAATTGATTATGCA+GGG + chr5.1:52244592-52244611 MS.gene21256:intron 40.0%
CCCTGCATAATCAATTCAGA+GGG - chr5.1:52244595-52244614 None:intergenic 40.0%
CCTCTGAATTGATTATGCAG+GGG + chr5.1:52244593-52244612 MS.gene21256:intron 40.0%
CGATTAATGAAGCACAGACA+CGG + chr5.1:52245670-52245689 MS.gene21256:intron 40.0%
CTGTACTATTTGTACTGCGT+TGG + chr5.1:52246126-52246145 MS.gene21256:intron 40.0%
GAATATGTTACATGGGGCTT+AGG + chr5.1:52246363-52246382 MS.gene21256:intron 40.0%
GCCGTAGTTAGACGAAATAA+TGG + chr5.1:52246240-52246259 MS.gene21256:intron 40.0%
GTTGCAGAACCATTAAGACT+TGG - chr5.1:52244736-52244755 None:intergenic 40.0%
TCAGGTCAATGTGAATCTCA+TGG + chr5.1:52245503-52245522 MS.gene21256:intron 40.0%
TCCCTCTGAATTGATTATGC+AGG + chr5.1:52244591-52244610 MS.gene21256:intron 40.0%
TGAAATAGGTGGTCCATGTA+AGG + chr5.1:52246009-52246028 MS.gene21256:intron 40.0%
TGGCATTAGAGTTTACTGCA+AGG + chr5.1:52244806-52244825 MS.gene21256:intron 40.0%
TTGAATGTAACCACGTGTGT+TGG + chr5.1:52245744-52245763 MS.gene21256:intron 40.0%
TTGATGAATCTTGTGTGGCA+GGG + chr5.1:52245148-52245167 MS.gene21256:intron 40.0%
! ATGTTCAGCTGTTTCACCAT+AGG + chr5.1:52245377-52245396 MS.gene21256:CDS 40.0%
! CCCATGTAGTTGAACTCTTT+TGG - chr5.1:52244075-52244094 None:intergenic 40.0%
! GACATCGTGGTTTTTGATGT+TGG - chr5.1:52245051-52245070 None:intergenic 40.0%
! GGCAACATTCTCAAGCATTT+TGG - chr5.1:52244635-52244654 None:intergenic 40.0%
! GTTGATGGAAAATTGGGATC+AGG + chr5.1:52245248-52245267 MS.gene21256:CDS 40.0%
! TGCCATGAATGTTTTCTCAG+AGG + chr5.1:52244368-52244387 MS.gene21256:CDS 40.0%
!! AGGTGTTGGAATTAGTTTGC+AGG - chr5.1:52246571-52246590 None:intergenic 40.0%
!! CAGTTAAGATTGGTCTTGAG+AGG + chr5.1:52244686-52244705 MS.gene21256:CDS 40.0%
!! GGTGTTGGAATTAGTTTGCA+GGG - chr5.1:52246570-52246589 None:intergenic 40.0%
!! TCATCTTTTTGAGGGTGCAA+AGG - chr5.1:52244294-52244313 None:intergenic 40.0%
!!! ACTTTTTTCAACTGCTCTGG+GGG - chr5.1:52246910-52246929 None:intergenic 40.0%
!!! GAATCAAAGCCTGCTGTTTT+AGG + chr5.1:52244324-52244343 MS.gene21256:CDS 40.0%
!!! TTCTCAAGCATTTTGGAGCT+AGG - chr5.1:52244628-52244647 None:intergenic 40.0%
AAAAGTTCGCAACCGAAGTC+TGG - chr5.1:52245079-52245098 None:intergenic 45.0%
AAAGTTCGCAACCGAAGTCT+GGG - chr5.1:52245078-52245097 None:intergenic 45.0%
AGATGGTGATTGGATGCTTG+TGG + chr5.1:52245601-52245620 MS.gene21256:CDS 45.0%
CACCATAGGTAGGTAGGAAT+AGG + chr5.1:52245391-52245410 MS.gene21256:intron 45.0%
CATCAAAAACCACGATGTCG+CGG + chr5.1:52245052-52245071 MS.gene21256:intron 45.0%
CCCCTGCATAATCAATTCAG+AGG - chr5.1:52244596-52244615 None:intergenic 45.0%
CTCAGATTGCGGTCAAATAG+TGG + chr5.1:52244942-52244961 MS.gene21256:intron 45.0%
CTCCTGCTAGCAAGTAAGTA+GGG + chr5.1:52244773-52244792 MS.gene21256:intron 45.0%
CTGTTTCACCATAGGTAGGT+AGG + chr5.1:52245385-52245404 MS.gene21256:intron 45.0%
CTTTCAGGAGAAAGTGATCC+TGG - chr5.1:52244213-52244232 None:intergenic 45.0%
GATGGAAAATTGGGATCAGG+AGG + chr5.1:52245251-52245270 MS.gene21256:CDS 45.0%
GATGGTGATTGGATGCTTGT+GGG + chr5.1:52245602-52245621 MS.gene21256:CDS 45.0%
GCTGAATGAAACCAAGCTGA+GGG + chr5.1:52245535-52245554 MS.gene21256:CDS 45.0%
GGCCTCCAATAAGATCGTTT+AGG + chr5.1:52245186-52245205 MS.gene21256:CDS 45.0%
GTAAGTAGGGTGGTGTGAAT+TGG + chr5.1:52244786-52244805 MS.gene21256:intron 45.0%
TCATCACCTATTCCTAGCCT+TGG - chr5.1:52243994-52244013 None:intergenic 45.0%
TCTCTACCAAGGCTAGGAAT+AGG + chr5.1:52243985-52244004 MS.gene21256:CDS 45.0%
TGCTGAATGAAACCAAGCTG+AGG + chr5.1:52245534-52245553 MS.gene21256:CDS 45.0%
TGTGTTTGTGAAGGTCAGCA+TGG + chr5.1:52245277-52245296 MS.gene21256:CDS 45.0%
TTGCTTTCACCGACACCATT+AGG - chr5.1:52244712-52244731 None:intergenic 45.0%
TTTGTGAAGGTCAGCATGGA+TGG + chr5.1:52245281-52245300 MS.gene21256:CDS 45.0%
! ATGAAGAGCTCTGATGCCAT+TGG + chr5.1:52246647-52246666 MS.gene21256:CDS 45.0%
! TCAGCTGTTTCACCATAGGT+AGG + chr5.1:52245381-52245400 MS.gene21256:intron 45.0%
! TGAGGGTGCAAAGGAAAAAG+AGG - chr5.1:52244285-52244304 None:intergenic 45.0%
!! AGCTCTGATGCCATTGGTTT+AGG + chr5.1:52246653-52246672 MS.gene21256:CDS 45.0%
!! ATTGGTCTTGAGAGGCCTAA+TGG + chr5.1:52244694-52244713 MS.gene21256:CDS 45.0%
!!! TCTAATTTTTTATAAAATAA+TGG + chr5.1:52244545-52244564 MS.gene21256:intron 5.0%
AAGCTGAGGGATCTGCTTCA+TGG + chr5.1:52245548-52245567 MS.gene21256:CDS 50.0%
ATGAAGCAGATCCCTCAGCT+TGG - chr5.1:52245549-52245568 None:intergenic 50.0%
CAATCTGAGCTGCGACATCA+TGG - chr5.1:52244931-52244950 None:intergenic 50.0%
CTGCTAGCAAGTAAGTAGGG+TGG + chr5.1:52244776-52244795 MS.gene21256:intron 50.0%
CTTGAGAGGCCTAATGGTGT+CGG + chr5.1:52244700-52244719 MS.gene21256:CDS 50.0%
GACACTGACATCGTCGACAT+TGG + chr5.1:52245697-52245716 MS.gene21256:intron 50.0%
GAGCTTAGATTGGGACTTCC+AGG + chr5.1:52244192-52244211 MS.gene21256:CDS 50.0%
GCTCCTGCTAGCAAGTAAGT+AGG + chr5.1:52244772-52244791 MS.gene21256:intron 50.0%
TGATGTCGCAGCTCAGATTG+CGG + chr5.1:52244931-52244950 MS.gene21256:intron 50.0%
TGCAGTCCGCAACTCAATTG+CGG - chr5.1:52244503-52244522 None:intergenic 50.0%
! ATCGGCTTTCACTTTCTGCC+CGG - chr5.1:52244656-52244675 None:intergenic 50.0%
! CACCCTACTTACTTGCTAGC+AGG - chr5.1:52244778-52244797 None:intergenic 50.0%
! TGCAACCCGCAATTGAGTTG+CGG + chr5.1:52244494-52244513 MS.gene21256:intron 50.0%
!! AGGAGGTGCTGTGTTTGTGA+AGG + chr5.1:52245268-52245287 MS.gene21256:CDS 50.0%
!! CACGTGTGTTGGTGTTGTGT+CGG + chr5.1:52245755-52245774 MS.gene21256:intron 50.0%
AATCTTGTGTGGCAGGGCAC+AGG + chr5.1:52245154-52245173 MS.gene21256:intron 55.0%
CGACACAACACCAACACACG+TGG - chr5.1:52245757-52245776 None:intergenic 55.0%
GCAGTCCGCAACTCAATTGC+GGG - chr5.1:52244502-52244521 None:intergenic 55.0%
GCTTGTGGGTGATGTTCCCT+GGG + chr5.1:52245616-52245635 MS.gene21256:CDS 55.0%
TGCTTGTGGGTGATGTTCCC+TGG + chr5.1:52245615-52245634 MS.gene21256:CDS 55.0%
GCAGGGCACAGGTTGTTGGT+TGG + chr5.1:52245165-52245184 MS.gene21256:intron 60.0%
TGTGGCAGGGCACAGGTTGT+TGG + chr5.1:52245161-52245180 MS.gene21256:intron 60.0%
!! CTGCTCTGGGGGCTGCAAAA+TGG - chr5.1:52246899-52246918 None:intergenic 60.0%
AAGTCTGGGCCGCGACATCG+TGG - chr5.1:52245064-52245083 None:intergenic 65.0%
CGATGTCGCGGCCCAGACTT+CGG + chr5.1:52245064-52245083 MS.gene21256:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 52243982 52246944 52243982 ID=MS.gene21256
chr5.1 mRNA 52243982 52246944 52243982 ID=MS.gene21256.t1;Parent=MS.gene21256
chr5.1 exon 52243982 52244389 52243982 ID=MS.gene21256.t1.exon1;Parent=MS.gene21256.t1
chr5.1 CDS 52243982 52244389 52243982 ID=cds.MS.gene21256.t1;Parent=MS.gene21256.t1
chr5.1 exon 52244613 52244785 52244613 ID=MS.gene21256.t1.exon2;Parent=MS.gene21256.t1
chr5.1 CDS 52244613 52244785 52244613 ID=cds.MS.gene21256.t1;Parent=MS.gene21256.t1
chr5.1 exon 52245169 52245398 52245169 ID=MS.gene21256.t1.exon3;Parent=MS.gene21256.t1
chr5.1 CDS 52245169 52245398 52245169 ID=cds.MS.gene21256.t1;Parent=MS.gene21256.t1
chr5.1 exon 52245507 52245639 52245507 ID=MS.gene21256.t1.exon4;Parent=MS.gene21256.t1
chr5.1 CDS 52245507 52245639 52245507 ID=cds.MS.gene21256.t1;Parent=MS.gene21256.t1
chr5.1 exon 52246613 52246674 52246613 ID=MS.gene21256.t1.exon5;Parent=MS.gene21256.t1
chr5.1 CDS 52246613 52246674 52246613 ID=cds.MS.gene21256.t1;Parent=MS.gene21256.t1
chr5.1 exon 52246907 52246944 52246907 ID=MS.gene21256.t1.exon6;Parent=MS.gene21256.t1
chr5.1 CDS 52246907 52246944 52246907 ID=cds.MS.gene21256.t1;Parent=MS.gene21256.t1
Gene Sequence

>MS.gene21256

ATGTCTCTACCAAGGCTAGGAATAGGTGATGAAGAAAGTAAAAACAATGTTACTTTGTTGGAAAAAAGTGTGTGTTTGAATGGTTCAAAACCAAAAGAGTTCAACTACATGGGATTATCATCCACTAATTGTTCATCAGTGGATAGTTCAGTTCCAAAAATTCAATCTTTTAAAGATGAGACCAAAAGTAATTTGAATCTTAAAGCTACTGAGCTTAGATTGGGACTTCCAGGATCACTTTCTCCTGAAAGAGATTCATCAGATTTTTGTTTAAGAAGTTCAAAGCAGTTTGATGAAAAGCCTCTTTTTCCTTTGCACCCTCAAAAAGATGATCATCTTTTTGAATCAAAGCCTGCTGTTTTAGGTAACAAAAGAGGGTTTTCTGATGCCATGAATGTTTTCTCAGAGGTAAATTGTCAATTCAATCGAAAGTGTATGATGGTTTTAAATTGCGGTTCAAGCTATGCTGCGAACTTTTATATTGCAGTTAAAATGCGGAAAAATGCTGTTTATGCAACCCGCAATTGAGTTGCGGACTGCAATTTAGAACTATCTATGATCTATCTAATTTTTTATAAAATAATGGAAGATTGTACTAAATATTAAAGTTCCCTCTGAATTGATTATGCAGGGGAAATTGAAGCCTAGCTCCAAAATGCTTGAGAATGTTGCCGGGCAGAAAGTGAAAGCCGATGAAATAGCAACAGTTAAGATTGGTCTTGAGAGGCCTAATGGTGTCGGTGAAAGCAAACCAAGTCTTAATGGTTCTGCAACTAATGGAAATAGCACTGCTCCTGCTAGCAAGTAAGTAGGGTGGTGTGAATTGGCATTAGAGTTTACTGCAAGGTTTTAAATTTAGGACATGTTACGTTTTTTGATACTGCGAATCAAATGCTGTTGATGCAGCCTGAATCGCAGTTACTTACAAATGCGGCGACATCAAAAGCCATGATGTCGCAGCTCAGATTGCGGTCAAATAGTGGCCGCATTTCAATTTTTGTGATTTTTGAGAGTCATAGTCAAATGCAGCTGATGCAGCTTGAATCACGATTGTATCACGGTCATGCCAACATCAAAAACCACGATGTCGCGGCCCAGACTTCGGTTGCGAACTTTTTTTAGAAGCTTGGTTTGGCTTAACAATGAAAATGAACTCGGAATGATGATTGATGAATCTTGTGTGGCAGGGCACAGGTTGTTGGTTGGCCTCCAATAAGATCGTTTAGGAAAAATTCATTGACCGCTGCTTCAAAGAACAATGAAGAGGTTGATGGAAAATTGGGATCAGGAGGTGCTGTGTTTGTGAAGGTCAGCATGGATGGTGCTCCGTATTTGAGAAAAGTAGACTTGAAGAATTACACTGCATATTCACAACTATCTTCTTCTCTTGAGAAGATGTTCAGCTGTTTCACCATAGGTAGGTAGGAATAGGTTCATTTTTGAATAAAAGAAAAATAGTCTTTCAACGATTTGAGGCAATTTTTGTGTCTGGATCAAGTTACTGCTTATGTTGATGTGATTTCAGGTCAATGTGAATCTCATGGAAATCAGATGCTGAATGAAACCAAGCTGAGGGATCTGCTTCATGGTTCAGAATATGTTATTACTTATGAGGATAAAGATGGTGATTGGATGCTTGTGGGTGATGTTCCCTGGGAGTAAGTTTATGTACTTGGTTTTCTTTCATGCGATTAATGAAGCACAGACACGGATATGACACTGACATCGTCGACATTGGTAGTATGTTTAGAAAATAGAATAATTGAATGTAACCACGTGTGTTGGTGTTGTGTCGGCACACATTCAATCTACATCTACATAAGTATACATCATCAACATTCTACGTAGATAAGTTTTAGACAAACGATTAATCACATTTTATTATCTTATCACGGCGTAAACACATAATGAAGTAGTTAAACTTATGTGTAAAATTTATTTATACAGTCTGATTATGCTGATAAAAATAATGAGTTGTTTTAAAATAATTAGAGAAATACAAATTTTGCAGCATTGTGGTTTTAGTTTGAAATAGGTGGTCCATGTAAGGTATATTAATAGAAGGTAAGAAACTGCCATTTTTGGCATTATTGAAGACTGTACAAAAGCAATAAAAGCTTGAATGATAATGGATATAAACCATACTGTACTATTTGTACTGCGTTGGTGACATGTGAGTATAATCAGAACAATGTGACTTCAAATTAAAATGGACCAACAATTTAAGCCAATTCAAAACTTAGCGCTTGTTTAGATTAGCCGTAGTTAGACGAAATAATGGTGTAGCACAATAATTTTGACATAAGTTACACTTTAGAGCTTAAACAAAATCGCAGTGCTACCATGATTCCAATTTCCAAACATGCAGTCATACACTAAATGAATATGTTACATGGGGCTTAGGATTTTGATTATCCTGTTTTCAATTTTGCGTTTCATAGTATTTATTGCTTATTTCGTAAATAATCCATAGCGAATTTGATACGATTTTATTGAAAGGGATGAGGAAAATTCTTAGAGAGGGTCTATGTTTATAAACAAAGTTTTACTAACCTTGTTTACTTCACTTTGATTTTATATCTGCACCCTGCAAACTAATTCCAACACCTAATATATCTCAACTCTTTGCAGGATGTTTATTGATACATGCAGGAGGCTAAGAATCATGAAGAGCTCTGATGCCATTGGTTTAGGTTAGTATTCCATTCATTTAACACAATCAATCTTAAAAATTGTATTGCATATATATACTAAATGATCTTAAAAAAAAGAGATATTTGTTCACTTATCACAAAATAACAGAATCCTACTTCAACTTAATTTACTTTGCCAATTTCTATTTTTGATAAAAATTTTATATATATTATACTATATGTTAGCATTTCCAATTCAAGTACAAATTATGTGTTGTGGTCCATTTTGCAGCCCCCAGAGCAGTTGAAAAAAGTAAAAGCAGGAACTAA
Protein sequence

>MS.gene21256.t1

MSLPRLGIGDEESKNNVTLLEKSVCLNGSKPKEFNYMGLSSTNCSSVDSSVPKIQSFKDETKSNLNLKATELRLGLPGSLSPERDSSDFCLRSSKQFDEKPLFPLHPQKDDHLFESKPAVLGNKRGFSDAMNVFSEGKLKPSSKMLENVAGQKVKADEIATVKIGLERPNGVGESKPSLNGSATNGNSTAPASKAQVVGWPPIRSFRKNSLTAASKNNEEVDGKLGSGGAVFVKVSMDGAPYLRKVDLKNYTAYSQLSSSLEKMFSCFTIGQCESHGNQMLNETKLRDLLHGSEYVITYEDKDGDWMLVGDVPWEMFIDTCRRLRIMKSSDAIGLAPRAVEKSKSRN