Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058210.t1 | XP_003594789.1 | 100 | 193 | 0 | 0 | 1 | 193 | 1 | 193 | 6.00E-107 | 396.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058210.t1 | O04834 | 96.4 | 193 | 7 | 0 | 1 | 193 | 1 | 193 | 1.5e-106 | 386.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058210.t1 | G7IMU5 | 100.0 | 193 | 0 | 0 | 1 | 193 | 1 | 193 | 4.3e-107 | 396.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050864 | MS.gene058210 | 0.800677 | 1.29E-48 | -1.69E-46 |
| MS.gene051234 | MS.gene058210 | 0.816992 | 4.15E-52 | -1.69E-46 |
| MS.gene058149 | MS.gene058210 | 0.803456 | 3.46E-49 | -1.69E-46 |
| MS.gene058210 | MS.gene059778 | 0.800052 | 1.73E-48 | -1.69E-46 |
| MS.gene058210 | MS.gene061707 | 0.802506 | 5.43E-49 | -1.69E-46 |
| MS.gene058210 | MS.gene061773 | 0.832267 | 1.04E-55 | -1.69E-46 |
| MS.gene058210 | MS.gene061774 | 0.829238 | 5.76E-55 | -1.69E-46 |
| MS.gene058210 | MS.gene061775 | 0.813376 | 2.64E-51 | -1.69E-46 |
| MS.gene058210 | MS.gene063145 | 0.810775 | 9.74E-51 | -1.69E-46 |
| MS.gene058210 | MS.gene063667 | 0.801284 | 9.68E-49 | -1.69E-46 |
| MS.gene058210 | MS.gene064622 | 0.804679 | 1.92E-49 | -1.69E-46 |
| MS.gene058210 | MS.gene065674 | 0.806274 | 8.91E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene066010 | 0.828189 | 1.03E-54 | -1.69E-46 |
| MS.gene058210 | MS.gene066196 | 0.809249 | 2.08E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene067556 | 0.836672 | 8.16E-57 | -1.69E-46 |
| MS.gene058210 | MS.gene072351 | 0.801706 | 7.94E-49 | -1.69E-46 |
| MS.gene058210 | MS.gene074317 | 0.818101 | 2.34E-52 | -1.69E-46 |
| MS.gene058210 | MS.gene07723 | 0.807238 | 5.57E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene08826 | 0.810589 | 1.07E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene08980 | 0.808123 | 3.62E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene09266 | 0.800053 | 1.73E-48 | -1.69E-46 |
| MS.gene058210 | MS.gene09934 | 0.862843 | 3.80E-64 | -1.69E-46 |
| MS.gene058210 | MS.gene21256 | 0.802933 | 4.44E-49 | -1.69E-46 |
| MS.gene058210 | MS.gene22553 | 0.857863 | 1.22E-62 | -1.69E-46 |
| MS.gene058210 | MS.gene27333 | 0.809074 | 2.26E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene27646 | -0.805539 | 1.27E-49 | -1.69E-46 |
| MS.gene058210 | MS.gene38829 | 0.801959 | 7.04E-49 | -1.69E-46 |
| MS.gene058210 | MS.gene39623 | 0.824735 | 6.87E-54 | -1.69E-46 |
| MS.gene058210 | MS.gene51421 | 0.99519 | 7.32E-214 | -1.69E-46 |
| MS.gene058210 | MS.gene52996 | 0.840478 | 8.49E-58 | -1.69E-46 |
| MS.gene058210 | MS.gene55101 | 0.806144 | 9.49E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene55904 | 0.809399 | 1.93E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene56419 | 0.811201 | 7.88E-51 | -1.69E-46 |
| MS.gene058210 | MS.gene57289 | 0.800982 | 1.12E-48 | -1.69E-46 |
| MS.gene058210 | MS.gene58096 | 0.806276 | 8.90E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene58836 | 0.809891 | 1.51E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene61739 | 0.800299 | 1.54E-48 | -1.69E-46 |
| MS.gene058210 | MS.gene64142 | 0.817667 | 2.93E-52 | -1.69E-46 |
| MS.gene058210 | MS.gene67231 | 0.804426 | 2.17E-49 | -1.69E-46 |
| MS.gene058210 | MS.gene68200 | 0.820223 | 7.69E-53 | -1.69E-46 |
| MS.gene058210 | MS.gene68420 | 0.800483 | 1.41E-48 | -1.69E-46 |
| MS.gene058210 | MS.gene68793 | 0.817103 | 3.92E-52 | -1.69E-46 |
| MS.gene058210 | MS.gene70240 | 0.80968 | 1.68E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene70804 | 0.825699 | 4.07E-54 | -1.69E-46 |
| MS.gene058210 | MS.gene71698 | 0.806551 | 7.79E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene74606 | 0.837227 | 5.89E-57 | -1.69E-46 |
| MS.gene058210 | MS.gene83234 | 0.823845 | 1.11E-53 | -1.69E-46 |
| MS.gene058210 | MS.gene83466 | 0.810448 | 1.15E-50 | -1.69E-46 |
| MS.gene058210 | MS.gene89100 | 0.803958 | 2.72E-49 | -1.69E-46 |
| MS.gene058210 | MS.gene96190 | 0.802968 | 4.36E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058210.t1 | MTR_2g034640 | 100.000 | 193 | 0 | 0 | 1 | 193 | 1 | 193 | 3.34e-144 | 399 |
| MS.gene058210.t1 | MTR_4g121860 | 93.782 | 193 | 12 | 0 | 1 | 193 | 1 | 193 | 6.45e-138 | 383 |
| MS.gene058210.t1 | MTR_7g112090 | 92.746 | 193 | 14 | 0 | 1 | 193 | 1 | 193 | 8.41e-137 | 380 |
| MS.gene058210.t1 | MTR_4g128910 | 34.659 | 176 | 103 | 1 | 18 | 193 | 15 | 178 | 7.96e-29 | 106 |
| MS.gene058210.t1 | MTR_5g016540 | 32.768 | 177 | 103 | 2 | 18 | 192 | 15 | 177 | 5.51e-26 | 99.0 |
| MS.gene058210.t1 | MTR_5g007810 | 29.843 | 191 | 114 | 3 | 3 | 193 | 8 | 178 | 5.98e-25 | 96.3 |
| MS.gene058210.t1 | MTR_5g034130 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.07e-24 | 95.5 |
| MS.gene058210.t1 | MTR_5g034125 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.07e-24 | 95.5 |
| MS.gene058210.t1 | MTR_7g109960 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.13e-24 | 95.5 |
| MS.gene058210.t1 | MTR_1g086480 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.14e-24 | 95.5 |
| MS.gene058210.t1 | MTR_6g005820 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.16e-24 | 95.5 |
| MS.gene058210.t1 | MTR_4g077640 | 29.843 | 191 | 114 | 3 | 3 | 193 | 8 | 178 | 1.78e-24 | 95.1 |
| MS.gene058210.t1 | MTR_8g077540 | 33.333 | 171 | 103 | 1 | 22 | 192 | 18 | 177 | 8.14e-24 | 93.2 |
| MS.gene058210.t1 | MTR_4g026950 | 32.386 | 176 | 100 | 3 | 22 | 193 | 2 | 162 | 1.13e-23 | 92.4 |
| MS.gene058210.t1 | MTR_8g077540 | 33.333 | 171 | 103 | 1 | 22 | 192 | 44 | 203 | 1.75e-23 | 93.2 |
| MS.gene058210.t1 | MTR_5g007820 | 29.381 | 194 | 113 | 5 | 3 | 193 | 8 | 180 | 2.33e-23 | 92.0 |
| MS.gene058210.t1 | MTR_2g022200 | 37.267 | 161 | 83 | 4 | 3 | 151 | 1 | 155 | 3.97e-23 | 92.0 |
| MS.gene058210.t1 | MTR_5g015370 | 31.443 | 194 | 119 | 3 | 1 | 193 | 1 | 181 | 5.18e-23 | 91.3 |
| MS.gene058210.t1 | MTR_1g076550 | 33.333 | 171 | 102 | 1 | 19 | 189 | 16 | 174 | 2.33e-22 | 89.4 |
| MS.gene058210.t1 | MTR_5g078090 | 35.570 | 149 | 94 | 2 | 1 | 148 | 1 | 148 | 2.78e-22 | 89.4 |
| MS.gene058210.t1 | MTR_5g098820 | 87.500 | 40 | 5 | 0 | 39 | 78 | 1 | 40 | 6.12e-19 | 79.3 |
| MS.gene058210.t1 | MTR_5g098820 | 85.714 | 35 | 5 | 0 | 44 | 78 | 69 | 103 | 4.15e-15 | 69.3 |
| MS.gene058210.t1 | MTR_5g007840 | 29.861 | 144 | 81 | 3 | 3 | 146 | 8 | 131 | 1.17e-15 | 72.4 |
| MS.gene058210.t1 | MTR_8g077540 | 35.417 | 96 | 62 | 0 | 22 | 117 | 18 | 113 | 1.20e-15 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058210.t1 | AT4G02080 | 96.373 | 193 | 7 | 0 | 1 | 193 | 1 | 193 | 2.17e-140 | 389 |
| MS.gene058210.t1 | AT1G56330 | 95.833 | 192 | 8 | 0 | 1 | 192 | 1 | 192 | 8.69e-138 | 383 |
| MS.gene058210.t1 | AT3G62560 | 94.792 | 192 | 10 | 0 | 1 | 192 | 1 | 192 | 2.75e-137 | 381 |
| MS.gene058210.t1 | AT1G09180 | 91.192 | 193 | 17 | 0 | 1 | 193 | 1 | 193 | 2.69e-131 | 366 |
| MS.gene058210.t1 | AT1G02620 | 90.090 | 111 | 11 | 0 | 83 | 193 | 12 | 122 | 1.04e-71 | 213 |
| MS.gene058210.t1 | AT2G24765 | 35.227 | 176 | 102 | 1 | 18 | 193 | 15 | 178 | 1.19e-28 | 105 |
| MS.gene058210.t1 | AT3G49870 | 33.505 | 194 | 115 | 3 | 1 | 193 | 1 | 181 | 4.08e-27 | 101 |
| MS.gene058210.t1 | AT5G67560 | 32.990 | 194 | 116 | 3 | 1 | 193 | 1 | 181 | 4.13e-26 | 99.4 |
| MS.gene058210.t1 | AT3G49870 | 34.706 | 170 | 98 | 2 | 25 | 193 | 5 | 162 | 8.84e-26 | 97.8 |
| MS.gene058210.t1 | AT2G47170 | 31.551 | 187 | 112 | 2 | 8 | 192 | 5 | 177 | 5.73e-25 | 96.3 |
| MS.gene058210.t1 | AT2G47170 | 31.551 | 187 | 112 | 2 | 8 | 192 | 5 | 177 | 5.73e-25 | 96.3 |
| MS.gene058210.t1 | AT2G47170 | 31.551 | 187 | 112 | 2 | 8 | 192 | 5 | 177 | 5.73e-25 | 96.3 |
| MS.gene058210.t1 | AT3G62290 | 31.551 | 187 | 112 | 2 | 8 | 192 | 5 | 177 | 6.24e-25 | 96.3 |
| MS.gene058210.t1 | AT3G62290 | 31.551 | 187 | 112 | 2 | 8 | 192 | 5 | 177 | 6.24e-25 | 96.3 |
| MS.gene058210.t1 | AT3G62290 | 31.551 | 187 | 112 | 2 | 8 | 192 | 5 | 177 | 6.24e-25 | 96.3 |
| MS.gene058210.t1 | AT5G17060 | 30.366 | 191 | 113 | 3 | 3 | 193 | 8 | 178 | 7.97e-25 | 96.3 |
| MS.gene058210.t1 | AT5G17060 | 30.366 | 191 | 113 | 3 | 3 | 193 | 8 | 178 | 7.97e-25 | 96.3 |
| MS.gene058210.t1 | AT5G14670 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.12e-24 | 95.5 |
| MS.gene058210.t1 | AT5G14670 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.12e-24 | 95.5 |
| MS.gene058210.t1 | AT5G14670 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.26e-24 | 95.5 |
| MS.gene058210.t1 | AT1G10630 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.29e-24 | 95.1 |
| MS.gene058210.t1 | AT1G10630 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.29e-24 | 95.1 |
| MS.gene058210.t1 | AT1G70490 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.33e-24 | 95.1 |
| MS.gene058210.t1 | AT1G70490 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.33e-24 | 95.1 |
| MS.gene058210.t1 | AT1G70490 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.33e-24 | 95.1 |
| MS.gene058210.t1 | AT1G70490 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.33e-24 | 95.1 |
| MS.gene058210.t1 | AT1G70490 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.33e-24 | 95.1 |
| MS.gene058210.t1 | AT1G70490 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.33e-24 | 95.1 |
| MS.gene058210.t1 | AT1G23490 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.33e-24 | 95.1 |
| MS.gene058210.t1 | AT1G70490 | 31.638 | 177 | 105 | 2 | 18 | 192 | 15 | 177 | 1.33e-24 | 95.1 |
| MS.gene058210.t1 | AT2G18390 | 33.918 | 171 | 102 | 1 | 22 | 192 | 18 | 177 | 1.52e-24 | 95.1 |
| MS.gene058210.t1 | AT2G24765 | 41.593 | 113 | 66 | 0 | 18 | 130 | 15 | 127 | 2.02e-24 | 93.6 |
| MS.gene058210.t1 | AT3G03120 | 29.843 | 191 | 114 | 3 | 3 | 193 | 8 | 178 | 7.00e-24 | 93.6 |
| MS.gene058210.t1 | AT5G37680 | 35.948 | 153 | 96 | 2 | 1 | 152 | 1 | 152 | 2.87e-23 | 92.0 |
| MS.gene058210.t1 | AT3G49860 | 35.714 | 140 | 89 | 1 | 15 | 153 | 6 | 145 | 3.51e-23 | 91.3 |
| MS.gene058210.t1 | AT3G22950 | 32.759 | 174 | 105 | 2 | 19 | 192 | 16 | 177 | 9.32e-23 | 90.5 |
| MS.gene058210.t1 | AT3G22950 | 32.759 | 174 | 105 | 2 | 19 | 192 | 16 | 177 | 9.32e-23 | 90.5 |
| MS.gene058210.t1 | AT2G15310 | 30.508 | 177 | 111 | 1 | 16 | 192 | 13 | 177 | 1.55e-22 | 90.5 |
| MS.gene058210.t1 | AT3G03120 | 31.298 | 131 | 82 | 1 | 3 | 133 | 8 | 130 | 1.14e-20 | 84.0 |
| MS.gene058210.t1 | AT5G52210 | 36.913 | 149 | 76 | 3 | 15 | 148 | 7 | 152 | 2.80e-20 | 84.7 |
| MS.gene058210.t1 | AT5G52210 | 36.913 | 149 | 76 | 3 | 15 | 148 | 7 | 152 | 2.80e-20 | 84.7 |
| MS.gene058210.t1 | AT5G37680 | 39.806 | 103 | 62 | 0 | 50 | 152 | 3 | 105 | 1.33e-18 | 78.6 |
| MS.gene058210.t1 | AT1G02430 | 31.206 | 141 | 89 | 4 | 18 | 151 | 15 | 154 | 7.27e-15 | 69.3 |
| MS.gene058210.t1 | AT1G02430 | 31.206 | 141 | 89 | 4 | 18 | 151 | 15 | 154 | 1.38e-14 | 69.3 |
| MS.gene058210.t1 | AT1G02440 | 27.857 | 140 | 95 | 3 | 18 | 151 | 15 | 154 | 2.03e-13 | 65.9 |
Find 45 sgRNAs with CRISPR-Local
Find 146 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTTGCCTGTGGTGAAAT+TGG | 0.182813 | 2.4:+56123433 | None:intergenic |
| TTCATCTTCGATTGGTTCTA+TGG | 0.267772 | 2.4:-56125785 | MS.gene058210:CDS |
| ATCAAATTGCTCGTAGAGTT+TGG | 0.294302 | 2.4:-56125481 | MS.gene058210:CDS |
| AATGTTCCATTTCTTATTCT+AGG | 0.294978 | 2.4:-56123518 | MS.gene058210:CDS |
| CTTCGGAGGAATTGAGTATT+GGG | 0.328437 | 2.4:-56125538 | MS.gene058210:CDS |
| TTCTTGCATCCCTTGGTCTC+TGG | 0.356031 | 2.4:-56125760 | MS.gene058210:CDS |
| TATGGCATTCTTGCATCCCT+TGG | 0.385266 | 2.4:-56125767 | MS.gene058210:CDS |
| TCGTCTTCTGAAGCTGCATA+TGG | 0.388503 | 2.4:+56123479 | None:intergenic |
| CAACCATGTTCATCTTCGAT+TGG | 0.422638 | 2.4:-56125793 | None:intergenic |
| GAAGCCAAGATTCTCTTCCT+TGG | 0.425299 | 2.4:-56125731 | MS.gene058210:CDS |
| TTCCTTGGTCTTGATAATGC+TGG | 0.455864 | 2.4:-56125716 | MS.gene058210:CDS |
| TTGTTTCCTAGAATAAGAAA+TGG | 0.457150 | 2.4:+56123512 | None:intergenic |
| ACTTCGGAGGAATTGAGTAT+TGG | 0.465946 | 2.4:-56125539 | MS.gene058210:CDS |
| CATCTTTGAGCATATGAAGA+AGG | 0.466211 | 2.4:+56125684 | None:intergenic |
| ATTGTCCGCAAGATGGGATA+TGG | 0.471519 | 2.4:-56123356 | MS.gene058210:CDS |
| TTCCTCCGAAGTAGGATGTT+GGG | 0.479835 | 2.4:+56125550 | None:intergenic |
| TGCAGCATTGTCCGCAAGAT+GGG | 0.482538 | 2.4:-56123362 | MS.gene058210:CDS |
| TTGGAAAGATTATTATGCTA+AGG | 0.487117 | 2.4:-56125462 | MS.gene058210:intron |
| AAGACCAAGGAAGAGAATCT+TGG | 0.490940 | 2.4:+56125727 | None:intergenic |
| TGATATATTGTGAAAGCCAC+TGG | 0.515926 | 2.4:+56123324 | None:intergenic |
| ACCAATTTCACCACAGGCAA+AGG | 0.519780 | 2.4:-56123434 | MS.gene058210:CDS |
| CGTCTTCTGAAGCTGCATAT+GGG | 0.521363 | 2.4:+56123480 | None:intergenic |
| GTGCAGCATTGTCCGCAAGA+TGG | 0.523551 | 2.4:-56123363 | MS.gene058210:CDS |
| TGCCTGTGGTGAAATTGGTT+AGG | 0.535024 | 2.4:+56123438 | None:intergenic |
| GAGGTTTGCAGAATCTAAGA+AGG | 0.538939 | 2.4:-56123576 | MS.gene058210:CDS |
| ATACTCAATTCCTCCGAAGT+AGG | 0.542870 | 2.4:+56125542 | None:intergenic |
| ATTCCTCCGAAGTAGGATGT+TGG | 0.553310 | 2.4:+56125549 | None:intergenic |
| ATTGACCTTGCCTTTGCCTG+TGG | 0.559718 | 2.4:+56123424 | None:intergenic |
| CGCAAGATGGGATATGGCGA+AGG | 0.565211 | 2.4:-56123350 | MS.gene058210:CDS |
| TCTTCATATGCTCAAAGATG+AGG | 0.580955 | 2.4:-56125681 | MS.gene058210:intron |
| ATCTTTGAGCATATGAAGAA+GGG | 0.582029 | 2.4:+56125685 | None:intergenic |
| CTTTGTCGTAAGCATCGACT+AGG | 0.589660 | 2.4:+56123600 | None:intergenic |
| GGCCTAACCAATTTCACCAC+AGG | 0.595827 | 2.4:-56123440 | MS.gene058210:CDS |
| CTGCACATGAACACTTCAAG+AGG | 0.596703 | 2.4:+56123380 | None:intergenic |
| TCGATGCTTACGACAAAGAG+AGG | 0.612300 | 2.4:-56123595 | MS.gene058210:CDS |
| ACCGACCCAACATCCTACTT+CGG | 0.614242 | 2.4:-56125555 | MS.gene058210:CDS |
| TTCCAGCATTATCAAGACCA+AGG | 0.615002 | 2.4:+56125714 | None:intergenic |
| GACGAGCTACGATATCATCT+CGG | 0.618823 | 2.4:-56123461 | MS.gene058210:CDS |
| TCCGAAGTAGGATGTTGGGT+CGG | 0.619899 | 2.4:+56125554 | None:intergenic |
| CACTTCAAGAGGTCGAACGT+TGG | 0.639582 | 2.4:+56123391 | None:intergenic |
| TTTCACCACAGGCAAAGGCA+AGG | 0.642687 | 2.4:-56123429 | MS.gene058210:CDS |
| CTTCGCCATATCCCATCTTG+CGG | 0.645914 | 2.4:+56123351 | None:intergenic |
| GAACCAATCGAAGATGAACA+TGG | 0.652038 | 2.4:+56125790 | None:intergenic |
| GACCCAACATCCTACTTCGG+AGG | 0.659761 | 2.4:-56125552 | MS.gene058210:CDS |
| GATATGGCGAAGGCTTCCAG+TGG | 0.701402 | 2.4:-56123340 | MS.gene058210:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATATAATGATCTATCT+AGG | - | chr2.4:56124104-56124123 | MS.gene058210:intron | 15.0% |
| !! | ATAGATTATATCTGTATAAA+AGG | - | chr2.4:56124907-56124926 | MS.gene058210:intron | 15.0% |
| !!! | AAACTTCAAATTTTTGTTAT+TGG | + | chr2.4:56125293-56125312 | None:intergenic | 15.0% |
| !!! | ACAAAAATTTGAAGTTTTTT+TGG | - | chr2.4:56125296-56125315 | MS.gene058210:intron | 15.0% |
| !!! | ATTTCTTGATTATCATTTTT+TGG | - | chr2.4:56123684-56123703 | MS.gene058210:intron | 15.0% |
| !!! | CATTTTTTGGTTTTTATTTA+GGG | - | chr2.4:56123697-56123716 | MS.gene058210:intron | 15.0% |
| !!! | TCATTTTTTGGTTTTTATTT+AGG | - | chr2.4:56123696-56123715 | MS.gene058210:intron | 15.0% |
| !! | AACTATATTTCTTACAAAAG+TGG | + | chr2.4:56124295-56124314 | None:intergenic | 20.0% |
| !! | TTTGTATTTATCTGATTCAT+GGG | - | chr2.4:56124732-56124751 | MS.gene058210:intron | 20.0% |
| !!! | AAATCGAATTTTGAATCAAA+AGG | + | chr2.4:56123476-56123495 | None:intergenic | 20.0% |
| !!! | AAGTTTAAAGCTTTTGATTT+GGG | - | chr2.4:56123598-56123617 | MS.gene058210:CDS | 20.0% |
| !!! | AATCGAATTTTGAATCAAAA+GGG | + | chr2.4:56123475-56123494 | None:intergenic | 20.0% |
| !!! | GCTATTTTTATAAACTATCT+TGG | + | chr2.4:56125124-56125143 | None:intergenic | 20.0% |
| !!! | TGATTTTCTCAAATTATTAG+TGG | - | chr2.4:56123945-56123964 | MS.gene058210:intron | 20.0% |
| ! | AATGTTCCATTTCTTATTCT+AGG | - | chr2.4:56125589-56125608 | MS.gene058210:intron | 25.0% |
| ! | ATAAAAATGTTACAAAGACC+AGG | + | chr2.4:56124047-56124066 | None:intergenic | 25.0% |
| ! | CTTTGTATTTATCTGATTCA+TGG | - | chr2.4:56124731-56124750 | MS.gene058210:intron | 25.0% |
| ! | GAGAAGATGAATTATTTGAA+TGG | + | chr2.4:56124440-56124459 | None:intergenic | 25.0% |
| ! | TTCAGTGTAATTATAAGTGT+CGG | + | chr2.4:56123920-56123939 | None:intergenic | 25.0% |
| ! | TTCTCTCAAATTCTTGTTAA+AGG | + | chr2.4:56124849-56124868 | None:intergenic | 25.0% |
| ! | TTGGAAAGATTATTATGCTA+AGG | - | chr2.4:56123645-56123664 | MS.gene058210:intron | 25.0% |
| ! | TTGTTTCCTAGAATAAGAAA+TGG | + | chr2.4:56125598-56125617 | None:intergenic | 25.0% |
| ! | TTTCTTACAAAAGTGGTATT+TGG | + | chr2.4:56124288-56124307 | None:intergenic | 25.0% |
| !! | AAATACAAAGAGCTTTATCT+GGG | + | chr2.4:56124721-56124740 | None:intergenic | 25.0% |
| !! | ACACTTTCATTTTCATGAAT+TGG | - | chr2.4:56124171-56124190 | MS.gene058210:intron | 25.0% |
| !! | CTCTAGAAGTTATAGTATAT+TGG | + | chr2.4:56125425-56125444 | None:intergenic | 25.0% |
| !! | TAAATACAAAGAGCTTTATC+TGG | + | chr2.4:56124722-56124741 | None:intergenic | 25.0% |
| !! | TTTTAATGACTCATTCTTCT+TGG | - | chr2.4:56124640-56124659 | MS.gene058210:intron | 25.0% |
| !!! | CAAGTTTAAAGCTTTTGATT+TGG | - | chr2.4:56123597-56123616 | MS.gene058210:CDS | 25.0% |
| !!! | GTTTTTATTTAGGGATTAGT+TGG | - | chr2.4:56123706-56123725 | MS.gene058210:intron | 25.0% |
| AACTAATTAACTAGCTAGCA+TGG | + | chr2.4:56124340-56124359 | None:intergenic | 30.0% | |
| ACACGTTCAATATATAGTGT+GGG | + | chr2.4:56124378-56124397 | None:intergenic | 30.0% | |
| ACTAATTAACTAGCTAGCAT+GGG | + | chr2.4:56124339-56124358 | None:intergenic | 30.0% | |
| ATCTTTGAGCATATGAAGAA+GGG | + | chr2.4:56123425-56123444 | None:intergenic | 30.0% | |
| ATTAAGCCGTTTATCTTAAC+AGG | - | chr2.4:56125233-56125252 | MS.gene058210:intron | 30.0% | |
| ATTTGTGAAAGTCTGTTGAA+AGG | - | chr2.4:56123749-56123768 | MS.gene058210:intron | 30.0% | |
| CATACCCTTGAATTGATATA+TGG | - | chr2.4:56124979-56124998 | MS.gene058210:intron | 30.0% | |
| TCAAAAGGGTCAAAGAAAAA+AGG | + | chr2.4:56123461-56123480 | None:intergenic | 30.0% | |
| TCAAATGTACTAATACGTGT+CGG | + | chr2.4:56124410-56124429 | None:intergenic | 30.0% | |
| TCATCTTCTCACATTATTAC+CGG | - | chr2.4:56124450-56124469 | MS.gene058210:intron | 30.0% | |
| TGTCTAAAGGTGAAAGAATA+TGG | + | chr2.4:56125260-56125279 | None:intergenic | 30.0% | |
| TTAAGCCGTTTATCTTAACA+GGG | - | chr2.4:56125234-56125253 | MS.gene058210:intron | 30.0% | |
| TTATGTTAGCTCAAACATGT+CGG | + | chr2.4:56125016-56125035 | None:intergenic | 30.0% | |
| ! | AATTTGTTTCAGCTATTGCA+TGG | - | chr2.4:56123779-56123798 | MS.gene058210:intron | 30.0% |
| ! | TGGAAATCTCTTTTGTCTAA+AGG | + | chr2.4:56125273-56125292 | None:intergenic | 30.0% |
| !! | ACTATAACTTCTAGAGTTGT+TGG | - | chr2.4:56125429-56125448 | MS.gene058210:intron | 30.0% |
| !!! | TTTAAAGCTTTTGATTTGGG+TGG | - | chr2.4:56123601-56123620 | MS.gene058210:CDS | 30.0% |
| AAACTATCTTGGAGAGCTTA+TGG | + | chr2.4:56125113-56125132 | None:intergenic | 35.0% | |
| AAAGGAAAACAGTACATGTG+TGG | + | chr2.4:56124831-56124850 | None:intergenic | 35.0% | |
| AAATCAGCACCTATGAAACA+CGG | + | chr2.4:56123993-56124012 | None:intergenic | 35.0% | |
| ACAGGCCATATATCAATTCA+AGG | + | chr2.4:56124987-56125006 | None:intergenic | 35.0% | |
| AGTCAAACATAGAGCTGAAT+TGG | + | chr2.4:56125363-56125382 | None:intergenic | 35.0% | |
| ATCAAATTGCTCGTAGAGTT+TGG | - | chr2.4:56123626-56123645 | MS.gene058210:intron | 35.0% | |
| CACACGTTCAATATATAGTG+TGG | + | chr2.4:56124379-56124398 | None:intergenic | 35.0% | |
| CAGGCCATATATCAATTCAA+GGG | + | chr2.4:56124986-56125005 | None:intergenic | 35.0% | |
| CATCTTTGAGCATATGAAGA+AGG | + | chr2.4:56123426-56123445 | None:intergenic | 35.0% | |
| CTATATATTGAACGTGTGTC+CGG | - | chr2.4:56124381-56124400 | MS.gene058210:intron | 35.0% | |
| GAGAGAAAGAACATACAGAA+GGG | - | chr2.4:56124862-56124881 | MS.gene058210:intron | 35.0% | |
| GTAACTACATGTGTAAGTGT+CGG | + | chr2.4:56124142-56124161 | None:intergenic | 35.0% | |
| GTATTTATCTGATTCATGGG+AGG | - | chr2.4:56124735-56124754 | MS.gene058210:intron | 35.0% | |
| TATGGCCCTGTTAAGATAAA+CGG | + | chr2.4:56125242-56125261 | None:intergenic | 35.0% | |
| TCTTCATATGCTCAAAGATG+AGG | - | chr2.4:56123426-56123445 | MS.gene058210:CDS | 35.0% | |
| TGAAAGTCTGTTGAAAGGTT+TGG | - | chr2.4:56123754-56123773 | MS.gene058210:intron | 35.0% | |
| TGAGAGAAAGAACATACAGA+AGG | - | chr2.4:56124861-56124880 | MS.gene058210:intron | 35.0% | |
| TGATATATTGTGAAAGCCAC+TGG | + | chr2.4:56125786-56125805 | None:intergenic | 35.0% | |
| ! | AGCTTTATCTGGGATTATGA+AGG | + | chr2.4:56124711-56124730 | None:intergenic | 35.0% |
| ! | TTTAGAATTCTCACCTATGC+TGG | - | chr2.4:56125459-56125478 | MS.gene058210:intron | 35.0% |
| !! | ACACCTCCTGAAAGTTTTAA+AGG | + | chr2.4:56124794-56124813 | None:intergenic | 35.0% |
| !! | ACTTCTAGAGTTGTTGGATA+TGG | - | chr2.4:56125435-56125454 | MS.gene058210:intron | 35.0% |
| !! | AGGTGTTACTTCTTGTTGTA+TGG | - | chr2.4:56124520-56124539 | MS.gene058210:intron | 35.0% |
| !! | GGTGTTACTTCTTGTTGTAT+GGG | - | chr2.4:56124521-56124540 | MS.gene058210:intron | 35.0% |
| !! | GTAATTATAAGTGTCGGTGT+CGG | + | chr2.4:56123914-56123933 | None:intergenic | 35.0% |
| !! | GTGTTACTTCTTGTTGTATG+GGG | - | chr2.4:56124522-56124541 | MS.gene058210:intron | 35.0% |
| !! | TTCATCTTCGATTGGTTCTA+TGG | - | chr2.4:56123322-56123341 | MS.gene058210:CDS | 35.0% |
| !!! | ACCTCCTGAAAGTTTTAAAG+GGG | + | chr2.4:56124792-56124811 | None:intergenic | 35.0% |
| !!! | CACCTCCTGAAAGTTTTAAA+GGG | + | chr2.4:56124793-56124812 | None:intergenic | 35.0% |
| !!! | CTGATTTGTTTTCAGTTGGA+TGG | - | chr2.4:56124006-56124025 | MS.gene058210:intron | 35.0% |
| AAGACCAAGGAAGAGAATCT+TGG | + | chr2.4:56123383-56123402 | None:intergenic | 40.0% | |
| ACTTCGGAGGAATTGAGTAT+TGG | - | chr2.4:56123568-56123587 | MS.gene058210:CDS | 40.0% | |
| AGAAAGAACATACAGAAGGG+TGG | - | chr2.4:56124865-56124884 | MS.gene058210:intron | 40.0% | |
| AGCTGAATTGGTGAATGGAA+AGG | + | chr2.4:56125351-56125370 | None:intergenic | 40.0% | |
| ATACTCAATTCCTCCGAAGT+AGG | + | chr2.4:56123568-56123587 | None:intergenic | 40.0% | |
| CAAACATGTCGGATCAAAAC+AGG | + | chr2.4:56125005-56125024 | None:intergenic | 40.0% | |
| CATAGAGCTGAATTGGTGAA+TGG | + | chr2.4:56125356-56125375 | None:intergenic | 40.0% | |
| CTTCGGAGGAATTGAGTATT+GGG | - | chr2.4:56123569-56123588 | MS.gene058210:CDS | 40.0% | |
| GAAAACAGTACATGTGTGGA+AGG | + | chr2.4:56124827-56124846 | None:intergenic | 40.0% | |
| GAAAGAACATACAGAAGGGT+GGG | - | chr2.4:56124866-56124885 | MS.gene058210:intron | 40.0% | |
| GAGGTTTGCAGAATCTAAGA+AGG | - | chr2.4:56125531-56125550 | MS.gene058210:CDS | 40.0% | |
| GGTCTATGTAAGTGCTTCAT+AGG | - | chr2.4:56124500-56124519 | MS.gene058210:intron | 40.0% | |
| TGTCTGTATCCGTGTTTCAT+AGG | - | chr2.4:56123981-56124000 | MS.gene058210:intron | 40.0% | |
| TTATTAGTGGTGTCTGTGTC+CGG | - | chr2.4:56123958-56123977 | MS.gene058210:intron | 40.0% | |
| TTCCAGCATTATCAAGACCA+AGG | + | chr2.4:56123396-56123415 | None:intergenic | 40.0% | |
| ! | CAGTGTTGTGTCTGTGATTT+TGG | - | chr2.4:56124205-56124224 | MS.gene058210:intron | 40.0% |
| ! | TTCTTTTTGCCAGAGACCAA+GGG | + | chr2.4:56123359-56123378 | None:intergenic | 40.0% |
| !! | ACCCCTTTAAAACTTTCAGG+AGG | - | chr2.4:56124788-56124807 | MS.gene058210:intron | 40.0% |
| !! | AGCACCCCTTTAAAACTTTC+AGG | - | chr2.4:56124785-56124804 | MS.gene058210:intron | 40.0% |
| !! | TTCCTTGGTCTTGATAATGC+TGG | - | chr2.4:56123391-56123410 | MS.gene058210:CDS | 40.0% |
| !!! | GGTGCTGATTTGTTTTCAGT+TGG | - | chr2.4:56124002-56124021 | MS.gene058210:intron | 40.0% |
| ACCAATTTCACCACAGGCAA+AGG | - | chr2.4:56125673-56125692 | MS.gene058210:intron | 45.0% | |
| AGACTGTTTGTGAGAGCTTG+TGG | - | chr2.4:56125045-56125064 | MS.gene058210:intron | 45.0% | |
| AGTTGGATGGATCTGTTGAC+AGG | - | chr2.4:56124019-56124038 | MS.gene058210:intron | 45.0% | |
| ATTCCTCCGAAGTAGGATGT+TGG | + | chr2.4:56123561-56123580 | None:intergenic | 45.0% | |
| ATTGTCCGCAAGATGGGATA+TGG | - | chr2.4:56125751-56125770 | MS.gene058210:CDS | 45.0% | |
| CATGGGAGGCAAAAAGTTAC+TGG | - | chr2.4:56124749-56124768 | MS.gene058210:intron | 45.0% | |
| CTGCACATGAACACTTCAAG+AGG | + | chr2.4:56125730-56125749 | None:intergenic | 45.0% | |
| GAAGCCAAGATTCTCTTCCT+TGG | - | chr2.4:56123376-56123395 | MS.gene058210:CDS | 45.0% | |
| GACGAGCTACGATATCATCT+CGG | - | chr2.4:56125646-56125665 | MS.gene058210:intron | 45.0% | |
| TACTAATACGTGTCGGACAC+CGG | + | chr2.4:56124403-56124422 | None:intergenic | 45.0% | |
| TATGGCATTCTTGCATCCCT+TGG | - | chr2.4:56123340-56123359 | MS.gene058210:CDS | 45.0% | |
| TCGATGCTTACGACAAAGAG+AGG | - | chr2.4:56125512-56125531 | MS.gene058210:CDS | 45.0% | |
| TGAAACACGGATACAGACAC+CGG | + | chr2.4:56123980-56123999 | None:intergenic | 45.0% | |
| TGCCTGTGGTGAAATTGGTT+AGG | + | chr2.4:56125672-56125691 | None:intergenic | 45.0% | |
| TTCCTCCGAAGTAGGATGTT+GGG | + | chr2.4:56123560-56123579 | None:intergenic | 45.0% | |
| ! | CAACGCTTGAGTGTTGTGTT+TGG | - | chr2.4:56124479-56124498 | MS.gene058210:intron | 45.0% |
| ! | CTTCTTTTTGCCAGAGACCA+AGG | + | chr2.4:56123360-56123379 | None:intergenic | 45.0% |
| !! | CGTCTTCTGAAGCTGCATAT+GGG | + | chr2.4:56125630-56125649 | None:intergenic | 45.0% |
| !! | CTTTGTCGTAAGCATCGACT+AGG | + | chr2.4:56125510-56125529 | None:intergenic | 45.0% |
| !! | TCGTCTTCTGAAGCTGCATA+TGG | + | chr2.4:56125631-56125650 | None:intergenic | 45.0% |
| !!! | GGGTGCTGGATTTTCATATG+TGG | - | chr2.4:56124542-56124561 | MS.gene058210:intron | 45.0% |
| ACACATCCACACATCCGACA+CGG | + | chr2.4:56123891-56123910 | None:intergenic | 50.0% | |
| ACCGACCCAACATCCTACTT+CGG | - | chr2.4:56123552-56123571 | MS.gene058210:CDS | 50.0% | |
| ATTGACCTTGCCTTTGCCTG+TGG | + | chr2.4:56125686-56125705 | None:intergenic | 50.0% | |
| CACTTCAAGAGGTCGAACGT+TGG | + | chr2.4:56125719-56125738 | None:intergenic | 50.0% | |
| CTTCGCCATATCCCATCTTG+CGG | + | chr2.4:56125759-56125778 | None:intergenic | 50.0% | |
| GCCTTTGCCTGTGGTGAAAT+TGG | + | chr2.4:56125677-56125696 | None:intergenic | 50.0% | |
| GGCCTAACCAATTTCACCAC+AGG | - | chr2.4:56125667-56125686 | MS.gene058210:intron | 50.0% | |
| TCCACCTGGATAACCAGCAT+AGG | + | chr2.4:56125475-56125494 | None:intergenic | 50.0% | |
| TCCGAAGTAGGATGTTGGGT+CGG | + | chr2.4:56123556-56123575 | None:intergenic | 50.0% | |
| TGCAGCATTGTCCGCAAGAT+GGG | - | chr2.4:56125745-56125764 | MS.gene058210:CDS | 50.0% | |
| TGGATCTGTTGACAGGTTCC+TGG | - | chr2.4:56124026-56124045 | MS.gene058210:intron | 50.0% | |
| TTCTTGCATCCCTTGGTCTC+TGG | - | chr2.4:56123347-56123366 | MS.gene058210:CDS | 50.0% | |
| TTTCACCACAGGCAAAGGCA+AGG | - | chr2.4:56125678-56125697 | MS.gene058210:intron | 50.0% | |
| ! | ACCTATGCTGGTTATCCAGG+TGG | - | chr2.4:56125471-56125490 | MS.gene058210:CDS | 50.0% |
| ! | CTCACCTATGCTGGTTATCC+AGG | - | chr2.4:56125468-56125487 | MS.gene058210:CDS | 50.0% |
| ! | CTTCTTGTTGTATGGGGTGC+TGG | - | chr2.4:56124528-56124547 | MS.gene058210:intron | 50.0% |
| ACATCCGACACGGCATGTGT+CGG | + | chr2.4:56123881-56123900 | None:intergenic | 55.0% | |
| CGCAAGATGGGATATGGCGA+AGG | - | chr2.4:56125757-56125776 | MS.gene058210:CDS | 55.0% | |
| GACCCAACATCCTACTTCGG+AGG | - | chr2.4:56123555-56123574 | MS.gene058210:CDS | 55.0% | |
| GATATGGCGAAGGCTTCCAG+TGG | - | chr2.4:56125767-56125786 | MS.gene058210:CDS | 55.0% | |
| GGTAAACTACAGCGTCCACC+TGG | + | chr2.4:56125489-56125508 | None:intergenic | 55.0% | |
| GTGCAGCATTGTCCGCAAGA+TGG | - | chr2.4:56125744-56125763 | MS.gene058210:CDS | 55.0% | |
| GTGTCGGACACATGGACATG+AGG | + | chr2.4:56123865-56123884 | None:intergenic | 55.0% | |
| !! | CACTCAAGCGTTGACATCGC+CGG | + | chr2.4:56124472-56124491 | None:intergenic | 55.0% |
| CACATGCCGTGTCGGATGTG+TGG | - | chr2.4:56123882-56123901 | MS.gene058210:intron | 60.0% | |
| CACGGCATGTGTCGGACACA+TGG | + | chr2.4:56123873-56123892 | None:intergenic | 60.0% | |
| GTGTCCGACACATGCCGTGT+CGG | - | chr2.4:56123874-56123893 | MS.gene058210:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 56123319 | 56125810 | 56123319 | ID=MS.gene058210 |
| chr2.4 | mRNA | 56123319 | 56125810 | 56123319 | ID=MS.gene058210.t1;Parent=MS.gene058210 |
| chr2.4 | exon | 56125682 | 56125810 | 56125682 | ID=MS.gene058210.t1.exon1;Parent=MS.gene058210.t1 |
| chr2.4 | CDS | 56125682 | 56125810 | 56125682 | ID=cds.MS.gene058210.t1;Parent=MS.gene058210.t1 |
| chr2.4 | exon | 56125463 | 56125594 | 56125463 | ID=MS.gene058210.t1.exon2;Parent=MS.gene058210.t1 |
| chr2.4 | CDS | 56125463 | 56125594 | 56125463 | ID=cds.MS.gene058210.t1;Parent=MS.gene058210.t1 |
| chr2.4 | exon | 56123319 | 56123639 | 56123319 | ID=MS.gene058210.t1.exon3;Parent=MS.gene058210.t1 |
| chr2.4 | CDS | 56123319 | 56123639 | 56123319 | ID=cds.MS.gene058210.t1;Parent=MS.gene058210.t1 |
| Gene Sequence |
| Protein sequence |
