Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21789.t1 | XP_024641881.1 | 95.1 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 3.30E-148 | 534.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21789.t1 | Q9XIP8 | 79.4 | 267 | 45 | 3 | 1 | 267 | 1 | 257 | 1.4e-126 | 453.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21789.t1 | G7KKM5 | 95.1 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 2.4e-148 | 534.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056633 | MS.gene21789 | -0.833545 | 5.01E-56 | -1.69E-46 |
| MS.gene058076 | MS.gene21789 | 0.899976 | 1.16E-77 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21789.t1 | MTR_5g007050 | 95.131 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 0.0 | 532 |
| MS.gene21789.t1 | MTR_6g046960 | 95.131 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 0.0 | 531 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21789.t1 | AT1G64980 | 78.277 | 267 | 55 | 2 | 3 | 267 | 12 | 277 | 3.54e-163 | 453 |
| MS.gene21789.t1 | AT1G64980 | 79.401 | 267 | 45 | 3 | 1 | 267 | 1 | 257 | 1.59e-162 | 451 |
| MS.gene21789.t1 | AT1G64980 | 79.401 | 267 | 45 | 3 | 1 | 267 | 1 | 257 | 1.59e-162 | 451 |
Find 60 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAGATCTGATTGCTTAAT+TGG | 0.085095 | 5.2:+1592642 | None:intergenic |
| TCCATCTTTGTAGTCTCTTT+TGG | 0.193307 | 5.2:+1592414 | None:intergenic |
| ATTGAAGACCAATTCTTTCT+AGG | 0.195136 | 5.2:+1592369 | None:intergenic |
| TCTCTATTTAGCTGATATTA+AGG | 0.242104 | 5.2:-1592496 | MS.gene21789:CDS |
| ACGTGAAGACATTGCATTTC+AGG | 0.259904 | 5.2:-1592715 | MS.gene21789:CDS |
| ATTTCCTTGAAGGACATAAT+AGG | 0.275347 | 5.2:-1592212 | MS.gene21789:CDS |
| AAGATTCTTAACACCTAGTT+TGG | 0.296665 | 5.2:-1592559 | MS.gene21789:CDS |
| TGGCTTGAGGATGATGAAAT+TGG | 0.336305 | 5.2:-1592255 | MS.gene21789:CDS |
| GGAGTAAGAACCTTGTTCTT+AGG | 0.346332 | 5.2:+1592324 | None:intergenic |
| TTTAGCTGATATTAAGGAAT+TGG | 0.346940 | 5.2:-1592490 | MS.gene21789:CDS |
| CTTTCCTTCATAGGTTTCAA+TGG | 0.348106 | 5.2:-1592275 | MS.gene21789:CDS |
| GAGGGCCGTGGTTTGAGGCT+TGG | 0.352682 | 5.2:-1592137 | MS.gene21789:CDS |
| AGACTGGTGCTTTCCTTCAT+AGG | 0.356440 | 5.2:-1592284 | MS.gene21789:CDS |
| TCGGGGAGGGCCGTGGTTTG+AGG | 0.366626 | 5.2:-1592142 | MS.gene21789:CDS |
| ACGGCCCTCCCCGAGTATAA+TGG | 0.377630 | 5.2:+1592151 | None:intergenic |
| ATTGCGAATTTGCTGATCTA+TGG | 0.387264 | 5.2:-1592110 | MS.gene21789:CDS |
| TTTCCTTGAAGGACATAATA+GGG | 0.389168 | 5.2:-1592211 | MS.gene21789:CDS |
| TACTAGTTGGTAATGGTGAT+TGG | 0.390769 | 5.2:+1592811 | None:intergenic |
| TAGAAAGAATTGGTCTTCAA+TGG | 0.393256 | 5.2:-1592367 | MS.gene21789:CDS |
| CAGCCCTATTATGTCCTTCA+AGG | 0.399988 | 5.2:+1592208 | None:intergenic |
| TTAATTGGTATGATCTCAAC+AGG | 0.402966 | 5.2:+1592657 | None:intergenic |
| TCAAGGTTGGGCTATGTTTG+TGG | 0.418527 | 5.2:-1592529 | MS.gene21789:CDS |
| CCTAGTTTGGCAAATTATCA+AGG | 0.430049 | 5.2:-1592546 | MS.gene21789:CDS |
| CCTTGATAATTTGCCAAACT+AGG | 0.430154 | 5.2:+1592546 | None:intergenic |
| TTTGGCAAATTATCAAGGTT+GGG | 0.439482 | 5.2:-1592541 | MS.gene21789:CDS |
| AAACCCTTCAAGATCTTTGT+GGG | 0.439682 | 5.2:-1592747 | MS.gene21789:CDS |
| GTCTGTGAATTCACAGCATC+AGG | 0.443576 | 5.2:+1592303 | None:intergenic |
| CTGTGTATCCTAGAAAGAAT+TGG | 0.452193 | 5.2:-1592377 | MS.gene21789:CDS |
| TTTGTTTGGAATTTCCTTGA+AGG | 0.455406 | 5.2:-1592222 | MS.gene21789:CDS |
| CGAGTATAATGGATAGCCTT+AGG | 0.455563 | 5.2:+1592162 | None:intergenic |
| GTTTGGCAAATTATCAAGGT+TGG | 0.457948 | 5.2:-1592542 | MS.gene21789:CDS |
| GAAACCCTTCAAGATCTTTG+TGG | 0.462717 | 5.2:-1592748 | MS.gene21789:CDS |
| ATTCCATTACCATTACTAGT+TGG | 0.470434 | 5.2:+1592798 | None:intergenic |
| TCAAGGAAATTCCAAACAAA+AGG | 0.473734 | 5.2:+1592225 | None:intergenic |
| ATTATACTCGGGGAGGGCCG+TGG | 0.478358 | 5.2:-1592149 | MS.gene21789:CDS |
| CTAAGGCTATCCATTATACT+CGG | 0.478464 | 5.2:-1592161 | MS.gene21789:CDS |
| GTCGTCATTCAATCATGAAA+AGG | 0.493012 | 5.2:-1592689 | MS.gene21789:CDS |
| TTACCATTACTAGTTGGTAA+TGG | 0.495217 | 5.2:+1592804 | None:intergenic |
| TCCAAAAGAGACTACAAAGA+TGG | 0.508455 | 5.2:-1592415 | MS.gene21789:CDS |
| GGAATTGGTAGATTTGATTG+AGG | 0.511326 | 5.2:-1592475 | MS.gene21789:CDS |
| TTACCAACTAGTAATGGTAA+TGG | 0.521531 | 5.2:-1592801 | MS.gene21789:CDS |
| GGTCTTTATTGGCGTGAAAG+AGG | 0.522858 | 5.2:-1592612 | MS.gene21789:CDS |
| CAAGCCATTGAAACCTATGA+AGG | 0.539155 | 5.2:+1592271 | None:intergenic |
| ATAACCCACAAAGATCTTGA+AGG | 0.541294 | 5.2:+1592743 | None:intergenic |
| TCATAGGTTTCAATGGCTTG+AGG | 0.551220 | 5.2:-1592268 | MS.gene21789:CDS |
| TTCTTCCAAGCCTCAAACCA+CGG | 0.559243 | 5.2:+1592132 | None:intergenic |
| TAAGGCTATCCATTATACTC+GGG | 0.565710 | 5.2:-1592160 | MS.gene21789:CDS |
| TGATCTATGGCTGAATGAGA+TGG | 0.566426 | 5.2:-1592097 | MS.gene21789:CDS |
| TAACCCACAAAGATCTTGAA+GGG | 0.577120 | 5.2:+1592744 | None:intergenic |
| CTGTGGTCATCCTAAGAACA+AGG | 0.579621 | 5.2:-1592334 | MS.gene21789:CDS |
| TGATCCAACTACTTCACCTA+AGG | 0.585896 | 5.2:-1592178 | MS.gene21789:CDS |
| TCACCATTACCAACTAGTAA+TGG | 0.590252 | 5.2:-1592807 | MS.gene21789:CDS |
| ATAGCCTTAGGTGAAGTAGT+TGG | 0.594477 | 5.2:+1592174 | None:intergenic |
| GATGCTGTGAATTCACAGAC+TGG | 0.623826 | 5.2:-1592300 | MS.gene21789:CDS |
| AAAGAGACTACAAAGATGGA+TGG | 0.623848 | 5.2:-1592411 | MS.gene21789:CDS |
| GCTATCCATTATACTCGGGG+AGG | 0.652245 | 5.2:-1592156 | MS.gene21789:CDS |
| ACAGAGATGAGTTTGAGCAA+TGG | 0.659058 | 5.2:-1592840 | None:intergenic |
| CTATCCATTATACTCGGGGA+GGG | 0.668674 | 5.2:-1592155 | MS.gene21789:CDS |
| AAGGCTATCCATTATACTCG+GGG | 0.680190 | 5.2:-1592159 | MS.gene21789:CDS |
| TGAAATGCAATGTCTTCACG+TGG | 0.692922 | 5.2:+1592717 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATCAGATTTTTCTTTTT+TGG | + | chr5.2:1592834-1592853 | None:intergenic | 15.0% |
| ! | TCTCTATTTAGCTGATATTA+AGG | - | chr5.2:1592391-1592410 | MS.gene21789:CDS | 25.0% |
| ! | TTTAGCTGATATTAAGGAAT+TGG | - | chr5.2:1592397-1592416 | MS.gene21789:CDS | 25.0% |
| AAGATTCTTAACACCTAGTT+TGG | - | chr5.2:1592328-1592347 | MS.gene21789:CDS | 30.0% | |
| ATTCCATTACCATTACTAGT+TGG | + | chr5.2:1592092-1592111 | None:intergenic | 30.0% | |
| ATTGAAGACCAATTCTTTCT+AGG | + | chr5.2:1592521-1592540 | None:intergenic | 30.0% | |
| ATTTCCTTGAAGGACATAAT+AGG | - | chr5.2:1592675-1592694 | MS.gene21789:CDS | 30.0% | |
| TCAAGGAAATTCCAAACAAA+AGG | + | chr5.2:1592665-1592684 | None:intergenic | 30.0% | |
| TGAGAAAAAGTGGTCTTTAT+TGG | - | chr5.2:1592264-1592283 | MS.gene21789:CDS | 30.0% | |
| TTAATTGGTATGATCTCAAC+AGG | + | chr5.2:1592233-1592252 | None:intergenic | 30.0% | |
| TTACCAACTAGTAATGGTAA+TGG | - | chr5.2:1592086-1592105 | MS.gene21789:CDS | 30.0% | |
| TTTCCTTGAAGGACATAATA+GGG | - | chr5.2:1592676-1592695 | MS.gene21789:CDS | 30.0% | |
| TTTGGCAAATTATCAAGGTT+GGG | - | chr5.2:1592346-1592365 | MS.gene21789:CDS | 30.0% | |
| ! | TTTGTTTGGAATTTCCTTGA+AGG | - | chr5.2:1592665-1592684 | MS.gene21789:CDS | 30.0% |
| !! | TAGAAAGAATTGGTCTTCAA+TGG | - | chr5.2:1592520-1592539 | MS.gene21789:CDS | 30.0% |
| !! | TTACCATTACTAGTTGGTAA+TGG | + | chr5.2:1592086-1592105 | None:intergenic | 30.0% |
| !!! | TCAATGGTTTTGTATAACTG+TGG | - | chr5.2:1592536-1592555 | MS.gene21789:CDS | 30.0% |
| AAACCCTTCAAGATCTTTGT+GGG | - | chr5.2:1592140-1592159 | MS.gene21789:CDS | 35.0% | |
| AAAGAGACTACAAAGATGGA+TGG | - | chr5.2:1592476-1592495 | MS.gene21789:CDS | 35.0% | |
| ATAACCCACAAAGATCTTGA+AGG | + | chr5.2:1592147-1592166 | None:intergenic | 35.0% | |
| CAATCAGATCTGAGAAAAAG+TGG | - | chr5.2:1592254-1592273 | MS.gene21789:CDS | 35.0% | |
| CCTAGTTTGGCAAATTATCA+AGG | - | chr5.2:1592341-1592360 | MS.gene21789:CDS | 35.0% | |
| CTAAGGCTATCCATTATACT+CGG | - | chr5.2:1592726-1592745 | MS.gene21789:CDS | 35.0% | |
| CTCAGATCTGATTGCTTAAT+TGG | + | chr5.2:1592248-1592267 | None:intergenic | 35.0% | |
| CTGTGTATCCTAGAAAGAAT+TGG | - | chr5.2:1592510-1592529 | MS.gene21789:CDS | 35.0% | |
| CTTTCCTTCATAGGTTTCAA+TGG | - | chr5.2:1592612-1592631 | MS.gene21789:CDS | 35.0% | |
| GGAATTGGTAGATTTGATTG+AGG | - | chr5.2:1592412-1592431 | MS.gene21789:CDS | 35.0% | |
| GTCGTCATTCAATCATGAAA+AGG | - | chr5.2:1592198-1592217 | MS.gene21789:CDS | 35.0% | |
| GTTTGGCAAATTATCAAGGT+TGG | - | chr5.2:1592345-1592364 | MS.gene21789:CDS | 35.0% | |
| TAACCCACAAAGATCTTGAA+GGG | + | chr5.2:1592146-1592165 | None:intergenic | 35.0% | |
| TAAGGCTATCCATTATACTC+GGG | - | chr5.2:1592727-1592746 | MS.gene21789:CDS | 35.0% | |
| TCACCATTACCAACTAGTAA+TGG | - | chr5.2:1592080-1592099 | MS.gene21789:CDS | 35.0% | |
| TCCAAAAGAGACTACAAAGA+TGG | - | chr5.2:1592472-1592491 | MS.gene21789:CDS | 35.0% | |
| ! | CCTTGATAATTTGCCAAACT+AGG | + | chr5.2:1592344-1592363 | None:intergenic | 35.0% |
| ! | TCCATCTTTGTAGTCTCTTT+TGG | + | chr5.2:1592476-1592495 | None:intergenic | 35.0% |
| !! | ATTGCGAATTTGCTGATCTA+TGG | - | chr5.2:1592777-1592796 | MS.gene21789:CDS | 35.0% |
| !! | TACTAGTTGGTAATGGTGAT+TGG | + | chr5.2:1592079-1592098 | None:intergenic | 35.0% |
| !!! | TTGGTTCTGTACCTTTTGTT+TGG | - | chr5.2:1592651-1592670 | MS.gene21789:CDS | 35.0% |
| !!! | TTTCTTTTTTGGCTTGAGCA+AGG | + | chr5.2:1592823-1592842 | None:intergenic | 35.0% |
| AAGGCTATCCATTATACTCG+GGG | - | chr5.2:1592728-1592747 | MS.gene21789:CDS | 40.0% | |
| ACGTGAAGACATTGCATTTC+AGG | - | chr5.2:1592172-1592191 | MS.gene21789:CDS | 40.0% | |
| CAAGCCATTGAAACCTATGA+AGG | + | chr5.2:1592619-1592638 | None:intergenic | 40.0% | |
| GAAACCCTTCAAGATCTTTG+TGG | - | chr5.2:1592139-1592158 | MS.gene21789:CDS | 40.0% | |
| GGAGTAAGAACCTTGTTCTT+AGG | + | chr5.2:1592566-1592585 | None:intergenic | 40.0% | |
| TCATAGGTTTCAATGGCTTG+AGG | - | chr5.2:1592619-1592638 | MS.gene21789:CDS | 40.0% | |
| TGAAATGCAATGTCTTCACG+TGG | + | chr5.2:1592173-1592192 | None:intergenic | 40.0% | |
| TGATCCAACTACTTCACCTA+AGG | - | chr5.2:1592709-1592728 | MS.gene21789:CDS | 40.0% | |
| TGATCTATGGCTGAATGAGA+TGG | - | chr5.2:1592790-1592809 | MS.gene21789:CDS | 40.0% | |
| TGGCTTGAGGATGATGAAAT+TGG | - | chr5.2:1592632-1592651 | MS.gene21789:CDS | 40.0% | |
| ! | ATAGCCTTAGGTGAAGTAGT+TGG | + | chr5.2:1592716-1592735 | None:intergenic | 40.0% |
| !! | CGAGTATAATGGATAGCCTT+AGG | + | chr5.2:1592728-1592747 | None:intergenic | 40.0% |
| AGACTGGTGCTTTCCTTCAT+AGG | - | chr5.2:1592603-1592622 | MS.gene21789:CDS | 45.0% | |
| CAGCCCTATTATGTCCTTCA+AGG | + | chr5.2:1592682-1592701 | None:intergenic | 45.0% | |
| CTATCCATTATACTCGGGGA+GGG | - | chr5.2:1592732-1592751 | MS.gene21789:CDS | 45.0% | |
| CTGTGGTCATCCTAAGAACA+AGG | - | chr5.2:1592553-1592572 | MS.gene21789:CDS | 45.0% | |
| GATGCTGTGAATTCACAGAC+TGG | - | chr5.2:1592587-1592606 | MS.gene21789:CDS | 45.0% | |
| GGTCTTTATTGGCGTGAAAG+AGG | - | chr5.2:1592275-1592294 | MS.gene21789:CDS | 45.0% | |
| GTCTGTGAATTCACAGCATC+AGG | + | chr5.2:1592587-1592606 | None:intergenic | 45.0% | |
| TCAAGGTTGGGCTATGTTTG+TGG | - | chr5.2:1592358-1592377 | MS.gene21789:CDS | 45.0% | |
| TTCTTCCAAGCCTCAAACCA+CGG | + | chr5.2:1592758-1592777 | None:intergenic | 45.0% | |
| GCTATCCATTATACTCGGGG+AGG | - | chr5.2:1592731-1592750 | MS.gene21789:CDS | 50.0% | |
| ACGGCCCTCCCCGAGTATAA+TGG | + | chr5.2:1592739-1592758 | None:intergenic | 60.0% | |
| ATTATACTCGGGGAGGGCCG+TGG | - | chr5.2:1592738-1592757 | MS.gene21789:CDS | 60.0% | |
| ! | GAGGGCCGTGGTTTGAGGCT+TGG | - | chr5.2:1592750-1592769 | MS.gene21789:CDS | 65.0% |
| TCGGGGAGGGCCGTGGTTTG+AGG | - | chr5.2:1592745-1592764 | MS.gene21789:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 1592053 | 1592856 | 1592053 | ID=MS.gene21789 |
| chr5.2 | mRNA | 1592053 | 1592856 | 1592053 | ID=MS.gene21789.t1;Parent=MS.gene21789 |
| chr5.2 | exon | 1592053 | 1592856 | 1592053 | ID=MS.gene21789.t1.exon1;Parent=MS.gene21789.t1 |
| chr5.2 | CDS | 1592053 | 1592856 | 1592053 | ID=cds.MS.gene21789.t1;Parent=MS.gene21789.t1 |
| Gene Sequence |
| Protein sequence |
