Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22512.t1 | XP_003625987.2 | 99.2 | 367 | 3 | 0 | 1 | 367 | 1 | 367 | 2.10E-209 | 738 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22512.t1 | Q6GQT9 | 36.9 | 374 | 205 | 10 | 6 | 367 | 8 | 362 | 3.8e-58 | 226.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22512.t1 | G7KTM5 | 99.2 | 367 | 3 | 0 | 1 | 367 | 1 | 367 | 1.5e-209 | 738.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049209 | MS.gene22512 | 0.831199 | 1.91E-55 | -1.69E-46 |
| MS.gene050348 | MS.gene22512 | 0.800474 | 1.42E-48 | -1.69E-46 |
| MS.gene052609 | MS.gene22512 | 0.831008 | 2.13E-55 | -1.69E-46 |
| MS.gene053143 | MS.gene22512 | 0.839564 | 1.47E-57 | -1.69E-46 |
| MS.gene054585 | MS.gene22512 | 0.834484 | 2.92E-56 | -1.69E-46 |
| MS.gene054824 | MS.gene22512 | 0.805157 | 1.53E-49 | -1.69E-46 |
| MS.gene056507 | MS.gene22512 | 0.804996 | 1.65E-49 | -1.69E-46 |
| MS.gene057213 | MS.gene22512 | 0.820636 | 6.18E-53 | -1.69E-46 |
| MS.gene059007 | MS.gene22512 | 0.802643 | 5.09E-49 | -1.69E-46 |
| MS.gene05938 | MS.gene22512 | 0.856418 | 3.26E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22512.t1 | MTR_7g109730 | 99.183 | 367 | 3 | 0 | 1 | 367 | 1 | 367 | 0.0 | 742 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22512.t1 | AT3G62360 | 71.271 | 362 | 102 | 1 | 8 | 367 | 11 | 372 | 0.0 | 546 |
| MS.gene22512.t1 | AT3G62360 | 71.191 | 361 | 102 | 1 | 8 | 366 | 11 | 371 | 0.0 | 545 |
Find 85 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTTGTAATTAAAGTTAA+TGG | 0.170602 | 7.2:+5896363 | MS.gene22512:CDS |
| TGTTGATGTTTAACATTAAC+TGG | 0.183876 | 7.2:-5900896 | None:intergenic |
| GTTATTCTTGTAGGTGGAAT+TGG | 0.221904 | 7.2:+5899828 | MS.gene22512:intron |
| TTTGGCATCCGTTTGTTTCC+TGG | 0.227971 | 7.2:-5895719 | None:intergenic |
| GCTTCTGCTGATTCCATCTA+TGG | 0.268002 | 7.2:+5895483 | MS.gene22512:CDS |
| TTCCAGGGAAACCCTATATT+AGG | 0.283328 | 7.2:+5900250 | MS.gene22512:intron |
| TGTTAACTGTTACAGGTAAC+TGG | 0.308644 | 7.2:+5902569 | MS.gene22512:intron |
| GAAACAAACGGATGCCAAAT+TGG | 0.314612 | 7.2:+5895723 | MS.gene22512:CDS |
| TGGAATTGGGCTTTGGAAAT+GGG | 0.315488 | 7.2:+5899842 | MS.gene22512:CDS |
| CTCTGTAGGTTCTCTATATC+TGG | 0.316967 | 7.2:+5896776 | MS.gene22512:intron |
| GTGGAATTGGGCTTTGGAAA+TGG | 0.325253 | 7.2:+5899841 | MS.gene22512:CDS |
| AATATATGGACTCCTAATAT+AGG | 0.326208 | 7.2:-5900262 | None:intergenic |
| GACATGTGAATAATCCAATT+TGG | 0.330019 | 7.2:-5895737 | None:intergenic |
| TGAACTAGTGCCCTACTATA+AGG | 0.331374 | 7.2:+5900835 | MS.gene22512:CDS |
| CCTCTGAATACAAGAATATA+TGG | 0.332768 | 7.2:-5900276 | None:intergenic |
| GATATGAAAGTTGAAGTTAA+AGG | 0.347337 | 7.2:+5899659 | MS.gene22512:CDS |
| ACATTAACTGGGACACTAGA+TGG | 0.348222 | 7.2:-5900884 | None:intergenic |
| ATCGCAGGCGAGTTCATCTT+TGG | 0.358289 | 7.2:+5895690 | MS.gene22512:intron |
| TTATTCTTGTAGGTGGAATT+GGG | 0.367599 | 7.2:+5899829 | MS.gene22512:intron |
| GTTGAACTATTGTCTCCTTC+TGG | 0.372002 | 7.2:+5896866 | MS.gene22512:CDS |
| TCTATATCTGGTAGAGTTGT+GGG | 0.404597 | 7.2:+5896788 | MS.gene22512:CDS |
| TTGTTTACAAATGTAATTCC+AGG | 0.405641 | 7.2:+5896932 | MS.gene22512:CDS |
| GAATCCACCGCAGCCATAGA+TGG | 0.409957 | 7.2:-5895496 | None:intergenic |
| TGTTTGCAAGGTTCTGCTAA+TGG | 0.419182 | 7.2:+5900316 | MS.gene22512:CDS |
| TTGTAGGTGGAATTGGGCTT+TGG | 0.422980 | 7.2:+5899835 | MS.gene22512:intron |
| ATATATGGACTCCTAATATA+GGG | 0.425298 | 7.2:-5900261 | None:intergenic |
| TGGCTGCGGTGGATTCGTTC+AGG | 0.426968 | 7.2:+5895503 | MS.gene22512:CDS |
| CTCTATATCTGGTAGAGTTG+TGG | 0.428279 | 7.2:+5896787 | MS.gene22512:CDS |
| AAGAGATAACAAACCTTCTC+AGG | 0.446642 | 7.2:-5896408 | None:intergenic |
| GAACTAGTGCCCTACTATAA+GGG | 0.463860 | 7.2:+5900836 | MS.gene22512:CDS |
| GGTAGAGTTGTGGGAGCTGC+AGG | 0.467417 | 7.2:+5896797 | MS.gene22512:CDS |
| ATGGACCTGATGGGTGGTCA+TGG | 0.468727 | 7.2:+5896382 | MS.gene22512:CDS |
| AGGTTATTACAAGCTTGATC+AGG | 0.485867 | 7.2:+5902691 | MS.gene22512:CDS |
| GGAATTGGGCTTTGGAAATG+GGG | 0.493886 | 7.2:+5899843 | MS.gene22512:CDS |
| GGAATAAAATAGTACCCGTT+AGG | 0.496115 | 7.2:-5896194 | None:intergenic |
| CTTGATCAGGTATTAGTATG+AGG | 0.497839 | 7.2:+5902704 | MS.gene22512:CDS |
| GCTTCAAACCGTGGATGGAT+TGG | 0.500914 | 7.2:+5896148 | MS.gene22512:CDS |
| CAGTGGGTCTGTTGTTGCAC+AGG | 0.503400 | 7.2:+5899903 | MS.gene22512:CDS |
| TCCATTAACTTTAATTACAA+AGG | 0.504858 | 7.2:-5896364 | None:intergenic |
| AACCGTGGATGGATTGGTAA+AGG | 0.509918 | 7.2:+5896154 | MS.gene22512:CDS |
| GAACACAATGTGCTCCTAAC+GGG | 0.513649 | 7.2:+5896180 | MS.gene22512:CDS |
| GGATCCCATGACCACCCATC+AGG | 0.514368 | 7.2:-5896387 | None:intergenic |
| GGTGTCAAGATCATAGTTGA+TGG | 0.525320 | 7.2:+5902641 | MS.gene22512:CDS |
| GTCCCTGGGTATAGTATCAG+TGG | 0.533507 | 7.2:+5899886 | MS.gene22512:CDS |
| GTTCATCTTTGGTGAAGTCC+AGG | 0.533922 | 7.2:+5895701 | MS.gene22512:CDS |
| GTTGAGCTTCAAACCGTGGA+TGG | 0.535663 | 7.2:+5896143 | MS.gene22512:CDS |
| GGTGAAGTCCAGGAAACAAA+CGG | 0.538937 | 7.2:+5895711 | MS.gene22512:CDS |
| ATCCTTTACCAATCCATCCA+CGG | 0.540905 | 7.2:-5896156 | None:intergenic |
| TGATGGAAACGACATGGGAG+TGG | 0.550160 | 7.2:+5902616 | MS.gene22512:CDS |
| AGGTCTATTACTGACAATCA+AGG | 0.558543 | 7.2:+5902671 | MS.gene22512:CDS |
| ATTAAAGTTAATGGACCTGA+TGG | 0.563941 | 7.2:+5896372 | MS.gene22512:CDS |
| TGGACCTGATGGGTGGTCAT+GGG | 0.566074 | 7.2:+5896383 | MS.gene22512:CDS |
| TCCCTGGGTATAGTATCAGT+GGG | 0.570104 | 7.2:+5899887 | MS.gene22512:CDS |
| CCATATATTCTTGTATTCAG+AGG | 0.578070 | 7.2:+5900276 | MS.gene22512:CDS |
| GCTGATTCCATCTATGGCTG+CGG | 0.580408 | 7.2:+5895489 | MS.gene22512:CDS |
| TTGTGAGGAATAGTAAACAT+AGG | 0.582720 | 7.2:-5895443 | None:intergenic |
| CCTGTGGTAGTTGACAATCA+AGG | 0.585283 | 7.2:+5896610 | MS.gene22512:CDS |
| GATTCCATCTATGGCTGCGG+TGG | 0.592582 | 7.2:+5895492 | MS.gene22512:CDS |
| AGAACACAATGTGCTCCTAA+CGG | 0.592633 | 7.2:+5896179 | MS.gene22512:CDS |
| ATTGAATGCGAACTTTCCAT+CGG | 0.594509 | 7.2:-5900380 | None:intergenic |
| CCTTGATTGTCAACTACCAC+AGG | 0.598295 | 7.2:-5896610 | None:intergenic |
| AGAGTTGTGGGAGCTGCAGG+CGG | 0.599360 | 7.2:+5896800 | MS.gene22512:CDS |
| GTGGTCATGGGATCCTGAGA+AGG | 0.604450 | 7.2:+5896395 | MS.gene22512:CDS |
| GTTGATGTTTAACATTAACT+GGG | 0.607862 | 7.2:-5900895 | None:intergenic |
| GCATTCAATTCAATACCTTG+TGG | 0.608043 | 7.2:+5900394 | MS.gene22512:CDS |
| CACGCTGTATCAGATGCCGA+TGG | 0.609383 | 7.2:+5900364 | MS.gene22512:CDS |
| TGGAAACGACATGGGAGTGG+AGG | 0.611254 | 7.2:+5902619 | MS.gene22512:CDS |
| CTCAGATCGGAAATGTCGAT+CGG | 0.614098 | 7.2:+5895411 | None:intergenic |
| GGAAACGACATGGGAGTGGA+GGG | 0.615099 | 7.2:+5902620 | MS.gene22512:CDS |
| CTTATCTGTATAGGTTCCTG+TGG | 0.615542 | 7.2:+5896594 | MS.gene22512:intron |
| TGTAGTTGATGGAAACGACA+TGG | 0.619360 | 7.2:+5902610 | MS.gene22512:CDS |
| CTGAAATTGAATAAGTTGTG+AGG | 0.620052 | 7.2:-5895458 | None:intergenic |
| TCATAGTTGATGGTCATGAA+AGG | 0.623919 | 7.2:+5902651 | MS.gene22512:CDS |
| TGAAGTTAAAGGTTCAACAC+AGG | 0.625903 | 7.2:+5899670 | MS.gene22512:CDS |
| TCCGAGGTAGAATGTTTGCA+AGG | 0.626426 | 7.2:+5900304 | MS.gene22512:CDS |
| TTAAAGTTAATGGACCTGAT+GGG | 0.630255 | 7.2:+5896373 | MS.gene22512:CDS |
| ACCTTGCAAACATTCTACCT+CGG | 0.635423 | 7.2:-5900305 | None:intergenic |
| ACCCACTGATACTATACCCA+GGG | 0.642433 | 7.2:-5899888 | None:intergenic |
| GACCCACTGATACTATACCC+AGG | 0.649163 | 7.2:-5899889 | None:intergenic |
| CAGAGAGCTACTTCTTGTCG+TGG | 0.655387 | 7.2:-5900340 | None:intergenic |
| GTAGGTTGAGCTTCAAACCG+TGG | 0.663440 | 7.2:+5896139 | MS.gene22512:intron |
| GTAGTTGATGGAAACGACAT+GGG | 0.665582 | 7.2:+5902611 | MS.gene22512:CDS |
| AAGTTAATGGACCTGATGGG+TGG | 0.666461 | 7.2:+5896376 | MS.gene22512:CDS |
| GATGACACAAGATCGCCAGA+AGG | 0.666773 | 7.2:-5896881 | None:intergenic |
| GTATTCAGAGGATGTGTCCG+AGG | 0.678153 | 7.2:+5900288 | MS.gene22512:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 5895423 | 5902724 | 5895423 | ID=MS.gene22512 |
| chr7.2 | mRNA | 5895423 | 5902724 | 5895423 | ID=MS.gene22512.t1;Parent=MS.gene22512 |
| chr7.2 | exon | 5895423 | 5895524 | 5895423 | ID=MS.gene22512.t1.exon1;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5895423 | 5895524 | 5895423 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| chr7.2 | exon | 5895697 | 5895762 | 5895697 | ID=MS.gene22512.t1.exon2;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5895697 | 5895762 | 5895697 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| chr7.2 | exon | 5896143 | 5896229 | 5896143 | ID=MS.gene22512.t1.exon3;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5896143 | 5896229 | 5896143 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| chr7.2 | exon | 5896360 | 5896416 | 5896360 | ID=MS.gene22512.t1.exon4;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5896360 | 5896416 | 5896360 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| chr7.2 | exon | 5896607 | 5896671 | 5896607 | ID=MS.gene22512.t1.exon5;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5896607 | 5896671 | 5896607 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| chr7.2 | exon | 5896784 | 5896953 | 5896784 | ID=MS.gene22512.t1.exon6;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5896784 | 5896953 | 5896784 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| chr7.2 | exon | 5899630 | 5899691 | 5899630 | ID=MS.gene22512.t1.exon7;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5899630 | 5899691 | 5899630 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| chr7.2 | exon | 5899841 | 5899924 | 5899841 | ID=MS.gene22512.t1.exon8;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5899841 | 5899924 | 5899841 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| chr7.2 | exon | 5900256 | 5900415 | 5900256 | ID=MS.gene22512.t1.exon9;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5900256 | 5900415 | 5900256 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| chr7.2 | exon | 5900828 | 5900943 | 5900828 | ID=MS.gene22512.t1.exon10;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5900828 | 5900943 | 5900828 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| chr7.2 | exon | 5902584 | 5902724 | 5902584 | ID=MS.gene22512.t1.exon11;Parent=MS.gene22512.t1 |
| chr7.2 | CDS | 5902584 | 5902724 | 5902584 | ID=cds.MS.gene22512.t1;Parent=MS.gene22512.t1 |
| Gene Sequence |
| Protein sequence |
