AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22556


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22556.t1 MTR_5g011800 95.652 230 9 1 4 233 1 229 2.77e-163 450
MS.gene22556.t1 MTR_5g011820 89.700 233 23 1 1 233 1 232 4.63e-154 427
MS.gene22556.t1 MTR_4g102220 58.475 236 81 6 4 233 1 225 2.78e-79 238
MS.gene22556.t1 MTR_4g024370 50.633 237 105 5 4 233 1 232 1.35e-73 223
MS.gene22556.t1 MTR_4g024370 50.633 237 105 5 4 233 1 232 1.35e-73 223
MS.gene22556.t1 MTR_6g060390 29.565 230 136 9 8 232 4 212 4.05e-23 93.6
MS.gene22556.t1 MTR_6g060440 30.000 230 133 10 8 232 4 210 2.93e-22 91.3
MS.gene22556.t1 MTR_1g076800 26.201 229 146 8 7 232 3 211 1.51e-19 84.0
MS.gene22556.t1 MTR_7g117380 27.948 229 151 7 8 232 3 221 1.24e-18 81.6
MS.gene22556.t1 MTR_4g023950 28.194 227 138 8 8 232 4 207 1.25e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22556.t1 AT2G45360 51.948 231 94 5 4 233 1 215 3.25e-72 219
MS.gene22556.t1 AT3G60780 49.785 233 99 6 4 233 1 218 5.50e-69 211
MS.gene22556.t1 AT1G62840 46.888 241 100 8 4 233 1 224 2.52e-54 174
MS.gene22556.t1 AT1G12320 42.857 231 104 7 4 229 1 208 6.11e-45 149

Find 68 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGGGATGTTCACTTTATT+AGG 0.233385 5.2:-4370034 MS.gene22556:CDS
TTTATTTCTAAGATGGATTC+TGG 0.260989 5.2:-4370245 MS.gene22556:CDS
TATGAGTTAGTTGCTCTCTC+TGG 0.291619 5.2:+4370680 None:intergenic
TTTGATTGGAATAGATTTCT+TGG 0.340143 5.2:-4370360 MS.gene22556:CDS
ATGGCGGAGCGTGGTGGCGC+GGG 0.351116 5.2:-4370216 MS.gene22556:CDS
AGATGGATTCTGGTTATATA+TGG 0.373750 5.2:-4370235 MS.gene22556:CDS
TGGTATGTCACCGGAAGTTT+TGG 0.381944 5.2:-4370411 MS.gene22556:CDS
ATTTCTTGGTTGTTGATTCT+CGG 0.396616 5.2:-4370346 MS.gene22556:CDS
TGAAAGACATGAAACTTGTT+TGG 0.407611 5.2:-4370706 MS.gene22556:CDS
TGTTCGGTCAGTATTTCTTC+CGG 0.416161 5.2:-4370180 MS.gene22556:CDS
GAAGTGATGGATGGTTTGAT+TGG 0.429509 5.2:-4370374 MS.gene22556:CDS
ATGAGTTAGTTGCTCTCTCT+GGG 0.435811 5.2:+4370681 None:intergenic
AAGCATGTTGATCAACGATC+AGG 0.439670 5.2:-4370053 MS.gene22556:CDS
TCCGATCTTGACCGTTCATC+AGG 0.440907 5.2:+4370458 None:intergenic
AGAATCCGGTGCGGCGGAGC+TGG 0.456364 5.2:-4370612 MS.gene22556:CDS
GGCGGAGCTGGTATCCTCTA+TGG 0.456798 5.2:-4370600 MS.gene22556:CDS
TGGATAAACCAACACTCGTT+GGG 0.457594 5.2:+4370522 None:intergenic
GGGGATCACGACGACTTGTT+CGG 0.470863 5.2:-4370196 MS.gene22556:CDS
ACCTGTGTGACCGCTTGCTA+TGG 0.473046 5.2:+4370502 None:intergenic
AGTATTTCTTCCGGTGGGGA+AGG 0.473923 5.2:-4370171 MS.gene22556:CDS
TTCTCTAGGAAGGTGATTGA+TGG 0.483897 5.2:+4370631 None:intergenic
TGTTACGGCGATGAAGCATA+AGG 0.490118 5.2:-4370102 MS.gene22556:CDS
AATCACCTTCCTAGAGAATC+CGG 0.490375 5.2:-4370626 MS.gene22556:CDS
AAGGTGATTGATGGTAGTTA+TGG 0.490442 5.2:+4370640 None:intergenic
GACGTGCTGTTCATAATAGA+TGG 0.491292 5.2:-4370079 MS.gene22556:CDS
CGGTCAGTATTTCTTCCGGT+GGG 0.491351 5.2:-4370176 MS.gene22556:CDS
GGTTCTCCTACCAAAACTTC+CGG 0.492682 5.2:+4370401 None:intergenic
TGGCGGAGCGTGGTGGCGCG+GGG 0.493800 5.2:-4370215 MS.gene22556:CDS
TTAGCAAAGTTGAGTGGTAA+AGG 0.497478 5.2:-4370296 MS.gene22556:CDS
TATGGCGGAGCGTGGTGGCG+CGG 0.506489 5.2:-4370217 MS.gene22556:CDS
CTGGTATCCTCTATGGCAGC+AGG 0.510352 5.2:-4370593 MS.gene22556:CDS
TGGTGGTGACAACTCTGTTA+CGG 0.515438 5.2:-4370117 MS.gene22556:CDS
AAACCATCCATCACTTCCTC+CGG 0.520767 5.2:+4370380 None:intergenic
AACAGAGTTGTCACCACCAC+CGG 0.522950 5.2:+4370121 None:intergenic
GGATACCAGCTCCGCCGCAC+CGG 0.538075 5.2:+4370607 None:intergenic
GTTACGGCGATGAAGCATAA+GGG 0.543192 5.2:-4370101 MS.gene22556:CDS
AGGAGAACCGGAGGAAGTGA+TGG 0.549491 5.2:-4370387 MS.gene22556:CDS
TCCTGATGAACGGTCAAGAT+CGG 0.549497 5.2:-4370459 MS.gene22556:CDS
GGTGGGGAAGGGAATTCATA+TGG 0.556013 5.2:-4370159 MS.gene22556:CDS
ATGGATAAACCAACACTCGT+TGG 0.556717 5.2:+4370521 None:intergenic
TCGGTCAGTATTTCTTCCGG+TGG 0.561365 5.2:-4370177 MS.gene22556:CDS
GTATTTCTTCCGGTGGGGAA+GGG 0.563160 5.2:-4370170 MS.gene22556:CDS
AGCATGTTGATCAACGATCA+GGG 0.563826 5.2:-4370052 MS.gene22556:CDS
TCCTAGAGAATCCGGTGCGG+CGG 0.565345 5.2:-4370618 MS.gene22556:CDS
GGAGTTATCCCAACGAGTGT+TGG 0.567790 5.2:-4370530 MS.gene22556:CDS
AGGATGGAACGCTCAGCTGA+TGG 0.575592 5.2:-4370573 MS.gene22556:CDS
GTTCCATCCTGCTGCCATAG+AGG 0.581085 5.2:+4370586 None:intergenic
ATATGAATTCCCTTCCCCAC+CGG 0.588515 5.2:+4370161 None:intergenic
TCCGCCGCACCGGATTCTCT+AGG 0.590144 5.2:+4370617 None:intergenic
CTCAGCTGATGGTAGAAACA+TGG 0.591953 5.2:-4370562 MS.gene22556:CDS
GGGAGAAGCTGGTATGTCAC+CGG 0.594972 5.2:-4370420 MS.gene22556:CDS
TTGAGGTTAGCAAAGTTGAG+TGG 0.609267 5.2:-4370302 MS.gene22556:CDS
TTATATATGGCGGAGCGTGG+TGG 0.612287 5.2:-4370222 MS.gene22556:CDS
CCGCACCGGATTCTCTAGGA+AGG 0.614758 5.2:+4370621 None:intergenic
GAACCGGAGGAAGTGATGGA+TGG 0.618455 5.2:-4370383 MS.gene22556:CDS
TCCATAGCAAGCGGTCACAC+AGG 0.622700 5.2:-4370503 MS.gene22556:CDS
GGTCAGTATTTCTTCCGGTG+GGG 0.623177 5.2:-4370175 MS.gene22556:CDS
TGGTTATATATGGCGGAGCG+TGG 0.623341 5.2:-4370225 MS.gene22556:CDS
TATCCTCTATGGCAGCAGGA+TGG 0.630751 5.2:-4370589 MS.gene22556:CDS
TGTGCATAGTTCCTGATGAA+CGG 0.633781 5.2:-4370469 MS.gene22556:CDS
ATAGCAAGCGGTCACACAGG+TGG 0.636263 5.2:-4370500 MS.gene22556:CDS
TGGATTCTGGTTATATATGG+CGG 0.643639 5.2:-4370232 MS.gene22556:CDS
GCATGTTGATCAACGATCAG+GGG 0.659538 5.2:-4370051 MS.gene22556:CDS
GTTGGTTTATCCATAGCAAG+CGG 0.671936 5.2:-4370512 MS.gene22556:CDS
ATGGTAGAAACATGGTCACA+CGG 0.678425 5.2:-4370554 MS.gene22556:CDS
TGAGTTAGTTGCTCTCTCTG+GGG 0.683268 5.2:+4370682 None:intergenic
CCTTCCTAGAGAATCCGGTG+CGG 0.793946 5.2:-4370621 MS.gene22556:CDS
GTAGAAACATGGTCACACGG+CGG 0.829401 5.2:-4370551 MS.gene22556:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGCTAATTTTATTTCTAAGA+TGG - chr5.2:4370483-4370502 MS.gene22556:CDS 20.0%
! TTTGATTGGAATAGATTTCT+TGG - chr5.2:4370375-4370394 MS.gene22556:CDS 25.0%
!!! TTTATTTCTAAGATGGATTC+TGG - chr5.2:4370490-4370509 MS.gene22556:CDS 25.0%
! AATTCATATGGCACACTTTT+CGG - chr5.2:4370588-4370607 MS.gene22556:CDS 30.0%
! AGATGGATTCTGGTTATATA+TGG - chr5.2:4370500-4370519 MS.gene22556:CDS 30.0%
! TGAAAGACATGAAACTTGTT+TGG - chr5.2:4370029-4370048 MS.gene22556:CDS 30.0%
!! ATTTCTTGGTTGTTGATTCT+CGG - chr5.2:4370389-4370408 MS.gene22556:CDS 30.0%
!!! AAGATTTTACAAGAGTGTTG+AGG - chr5.2:4370416-4370435 MS.gene22556:CDS 30.0%
ATCGGAGTATGCAAAAAACA+TGG - chr5.2:4370294-4370313 MS.gene22556:CDS 35.0%
TCGGAGTATGCAAAAAACAT+GGG - chr5.2:4370295-4370314 MS.gene22556:CDS 35.0%
TTAGCAAAGTTGAGTGGTAA+AGG - chr5.2:4370439-4370458 MS.gene22556:CDS 35.0%
! AAGGTGATTGATGGTAGTTA+TGG + chr5.2:4370098-4370117 None:intergenic 35.0%
! TGGATTCTGGTTATATATGG+CGG - chr5.2:4370503-4370522 MS.gene22556:CDS 35.0%
AAGCATGTTGATCAACGATC+AGG - chr5.2:4370682-4370701 MS.gene22556:CDS 40.0%
AATCACCTTCCTAGAGAATC+CGG - chr5.2:4370109-4370128 MS.gene22556:CDS 40.0%
AGCATGTTGATCAACGATCA+GGG - chr5.2:4370683-4370702 MS.gene22556:CDS 40.0%
ATGAGTTAGTTGCTCTCTCT+GGG + chr5.2:4370057-4370076 None:intergenic 40.0%
ATGGATAAACCAACACTCGT+TGG + chr5.2:4370217-4370236 None:intergenic 40.0%
ATGGTAGAAACATGGTCACA+CGG - chr5.2:4370181-4370200 MS.gene22556:CDS 40.0%
CAGGGGATGTTCACTTTATT+AGG - chr5.2:4370701-4370720 MS.gene22556:CDS 40.0%
TATGAGTTAGTTGCTCTCTC+TGG + chr5.2:4370058-4370077 None:intergenic 40.0%
TGGATAAACCAACACTCGTT+GGG + chr5.2:4370216-4370235 None:intergenic 40.0%
TGTGCATAGTTCCTGATGAA+CGG - chr5.2:4370266-4370285 MS.gene22556:CDS 40.0%
TGTTCGGTCAGTATTTCTTC+CGG - chr5.2:4370555-4370574 MS.gene22556:CDS 40.0%
TTGAGGTTAGCAAAGTTGAG+TGG - chr5.2:4370433-4370452 MS.gene22556:CDS 40.0%
! GACGTGCTGTTCATAATAGA+TGG - chr5.2:4370656-4370675 MS.gene22556:CDS 40.0%
! GTTGGTTTATCCATAGCAAG+CGG - chr5.2:4370223-4370242 MS.gene22556:CDS 40.0%
! TTCTCTAGGAAGGTGATTGA+TGG + chr5.2:4370107-4370126 None:intergenic 40.0%
!! GAAGTGATGGATGGTTTGAT+TGG - chr5.2:4370361-4370380 MS.gene22556:CDS 40.0%
AAACCATCCATCACTTCCTC+CGG + chr5.2:4370358-4370377 None:intergenic 45.0%
ATATGAATTCCCTTCCCCAC+CGG + chr5.2:4370577-4370596 None:intergenic 45.0%
CTCAGCTGATGGTAGAAACA+TGG - chr5.2:4370173-4370192 MS.gene22556:CDS 45.0%
GCAAAAAACATGGGAGAAGC+TGG - chr5.2:4370304-4370323 MS.gene22556:CDS 45.0%
GCATGTTGATCAACGATCAG+GGG - chr5.2:4370684-4370703 MS.gene22556:CDS 45.0%
GGTTCTCCTACCAAAACTTC+CGG + chr5.2:4370337-4370356 None:intergenic 45.0%
GTTACGGCGATGAAGCATAA+GGG - chr5.2:4370634-4370653 MS.gene22556:CDS 45.0%
TCCTGATGAACGGTCAAGAT+CGG - chr5.2:4370276-4370295 MS.gene22556:CDS 45.0%
TGAGTTAGTTGCTCTCTCTG+GGG + chr5.2:4370056-4370075 None:intergenic 45.0%
TGTTACGGCGATGAAGCATA+AGG - chr5.2:4370633-4370652 MS.gene22556:CDS 45.0%
! TGGTATGTCACCGGAAGTTT+TGG - chr5.2:4370324-4370343 MS.gene22556:CDS 45.0%
! TGGTGGTGACAACTCTGTTA+CGG - chr5.2:4370618-4370637 MS.gene22556:CDS 45.0%
!! ATGTCACCGGAAGTTTTGGT+AGG - chr5.2:4370328-4370347 MS.gene22556:CDS 45.0%
!! GGAAGTTTTGGTAGGAGAAC+CGG - chr5.2:4370336-4370355 MS.gene22556:CDS 45.0%
AACAGAGTTGTCACCACCAC+CGG + chr5.2:4370617-4370636 None:intergenic 50.0%
AGTATTTCTTCCGGTGGGGA+AGG - chr5.2:4370564-4370583 MS.gene22556:CDS 50.0%
CGGTCAGTATTTCTTCCGGT+GGG - chr5.2:4370559-4370578 MS.gene22556:CDS 50.0%
GGAGTTATCCCAACGAGTGT+TGG - chr5.2:4370205-4370224 MS.gene22556:CDS 50.0%
GGTCAGTATTTCTTCCGGTG+GGG - chr5.2:4370560-4370579 MS.gene22556:CDS 50.0%
GGTGGGGAAGGGAATTCATA+TGG - chr5.2:4370576-4370595 MS.gene22556:CDS 50.0%
GTAGAAACATGGTCACACGG+CGG - chr5.2:4370184-4370203 MS.gene22556:CDS 50.0%
GTATTTCTTCCGGTGGGGAA+GGG - chr5.2:4370565-4370584 MS.gene22556:CDS 50.0%
TATCCTCTATGGCAGCAGGA+TGG - chr5.2:4370146-4370165 MS.gene22556:CDS 50.0%
TCCGATCTTGACCGTTCATC+AGG + chr5.2:4370280-4370299 None:intergenic 50.0%
TCGGTCAGTATTTCTTCCGG+TGG - chr5.2:4370558-4370577 MS.gene22556:CDS 50.0%
! TGGTTATATATGGCGGAGCG+TGG - chr5.2:4370510-4370529 MS.gene22556:CDS 50.0%
! TTATATATGGCGGAGCGTGG+TGG - chr5.2:4370513-4370532 MS.gene22556:CDS 50.0%
!! AGTTTTGGTAGGAGAACCGG+AGG - chr5.2:4370339-4370358 MS.gene22556:CDS 50.0%
AGGAGAACCGGAGGAAGTGA+TGG - chr5.2:4370348-4370367 MS.gene22556:CDS 55.0%
AGGATGGAACGCTCAGCTGA+TGG - chr5.2:4370162-4370181 MS.gene22556:CDS 55.0%
ATAGCAAGCGGTCACACAGG+TGG - chr5.2:4370235-4370254 MS.gene22556:CDS 55.0%
CCTTCCTAGAGAATCCGGTG+CGG - chr5.2:4370114-4370133 MS.gene22556:CDS 55.0%
CTGGTATCCTCTATGGCAGC+AGG - chr5.2:4370142-4370161 MS.gene22556:CDS 55.0%
GGGAGAAGCTGGTATGTCAC+CGG - chr5.2:4370315-4370334 MS.gene22556:CDS 55.0%
GTTCCATCCTGCTGCCATAG+AGG + chr5.2:4370152-4370171 None:intergenic 55.0%
TCCATAGCAAGCGGTCACAC+AGG - chr5.2:4370232-4370251 MS.gene22556:CDS 55.0%
! ACCTGTGTGACCGCTTGCTA+TGG + chr5.2:4370236-4370255 None:intergenic 55.0%
! ATGGCACACTTTTCGGCTGC+CGG - chr5.2:4370595-4370614 MS.gene22556:CDS 55.0%
! GAACCGGAGGAAGTGATGGA+TGG - chr5.2:4370352-4370371 MS.gene22556:CDS 55.0%
! GGGGATCACGACGACTTGTT+CGG - chr5.2:4370539-4370558 MS.gene22556:CDS 55.0%
GGCGGAGCTGGTATCCTCTA+TGG - chr5.2:4370135-4370154 MS.gene22556:CDS 60.0%
TCCTAGAGAATCCGGTGCGG+CGG - chr5.2:4370117-4370136 MS.gene22556:CDS 60.0%
! CCGCACCGGATTCTCTAGGA+AGG + chr5.2:4370117-4370136 None:intergenic 60.0%
TCCGCCGCACCGGATTCTCT+AGG + chr5.2:4370121-4370140 None:intergenic 65.0%
! CACTTTTCGGCTGCCGGTGG+TGG - chr5.2:4370601-4370620 MS.gene22556:CDS 65.0%
! GCACACTTTTCGGCTGCCGG+TGG - chr5.2:4370598-4370617 MS.gene22556:CDS 65.0%
AGAATCCGGTGCGGCGGAGC+TGG - chr5.2:4370123-4370142 MS.gene22556:CDS 70.0%
GGATACCAGCTCCGCCGCAC+CGG + chr5.2:4370131-4370150 None:intergenic 70.0%
! TATGGCGGAGCGTGGTGGCG+CGG - chr5.2:4370518-4370537 MS.gene22556:CDS 70.0%
! ATGGCGGAGCGTGGTGGCGC+GGG - chr5.2:4370519-4370538 MS.gene22556:CDS 75.0%
!! TGGCGGAGCGTGGTGGCGCG+GGG - chr5.2:4370520-4370539 MS.gene22556:CDS 80.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 4370028 4370729 4370028 ID=MS.gene22556
chr5.2 mRNA 4370028 4370729 4370028 ID=MS.gene22556.t1;Parent=MS.gene22556
chr5.2 exon 4370028 4370729 4370028 ID=MS.gene22556.t1.exon1;Parent=MS.gene22556.t1
chr5.2 CDS 4370028 4370729 4370028 ID=cds.MS.gene22556.t1;Parent=MS.gene22556.t1
Gene Sequence

>MS.gene22556

ATGAAAGACATGAAACTTGTTTGGTCCCCAGAGAGAGCAACTAACTCATACATAGACACAGTTGAAGCCATAACTACCATCAATCACCTTCCTAGAGAATCCGGTGCGGCGGAGCTGGTATCCTCTATGGCAGCAGGATGGAACGCTCAGCTGATGGTAGAAACATGGTCACACGGCGGAGTTATCCCAACGAGTGTTGGTTTATCCATAGCAAGCGGTCACACAGGTGGAAGACACGTGTGCATAGTTCCTGATGAACGGTCAAGATCGGAGTATGCAAAAAACATGGGAGAAGCTGGTATGTCACCGGAAGTTTTGGTAGGAGAACCGGAGGAAGTGATGGATGGTTTGATTGGAATAGATTTCTTGGTTGTTGATTCTCGGAGAAAAGATTTTACAAGAGTGTTGAGGTTAGCAAAGTTGAGTGGTAAAGGTGCTGTGTTGATATGTAAGAATGCTAATTTTATTTCTAAGATGGATTCTGGTTATATATGGCGGAGCGTGGTGGCGCGGGGATCACGACGACTTGTTCGGTCAGTATTTCTTCCGGTGGGGAAGGGAATTCATATGGCACACTTTTCGGCTGCCGGTGGTGGTGACAACTCTGTTACGGCGATGAAGCATAAGGGACGTGCTGTTCATAATAGATGGATTAAGCATGTTGATCAACGATCAGGGGATGTTCACTTTATTAGGAAATGA

Protein sequence

>MS.gene22556.t1

MKDMKLVWSPERATNSYIDTVEAITTINHLPRESGAAELVSSMAAGWNAQLMVETWSHGGVIPTSVGLSIASGHTGGRHVCIVPDERSRSEYAKNMGEAGMSPEVLVGEPEEVMDGLIGIDFLVVDSRRKDFTRVLRLAKLSGKGAVLICKNANFISKMDSGYIWRSVVARGSRRLVRSVFLPVGKGIHMAHFSAAGGGDNSVTAMKHKGRAVHNRWIKHVDQRSGDVHFIRK