Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22718.t1 | XP_013450668.1 | 98 | 246 | 4 | 1 | 1 | 245 | 1 | 246 | 2.90E-139 | 505 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22718.t1 | Q8RV04 | 78.4 | 111 | 22 | 1 | 246 | 354 | 2 | 112 | 1.1e-43 | 178.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22718.t1 | I3SHV8 | 98.0 | 246 | 4 | 1 | 1 | 245 | 1 | 246 | 2.1e-139 | 505.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049697 | MS.gene22718 | 0.815019 | 1.15E-51 | -1.69E-46 |
| MS.gene050667 | MS.gene22718 | 0.973038 | 9.35E-136 | -1.69E-46 |
| MS.gene052169 | MS.gene22718 | 0.809896 | 1.51E-50 | -1.69E-46 |
| MS.gene05652 | MS.gene22718 | 0.802195 | 6.30E-49 | -1.69E-46 |
| MS.gene057669 | MS.gene22718 | 0.803653 | 3.15E-49 | -1.69E-46 |
| MS.gene057990 | MS.gene22718 | 0.810376 | 1.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22718.t1 | MTR_7g117750 | 97.571 | 247 | 5 | 1 | 1 | 246 | 1 | 247 | 0.0 | 507 |
| MS.gene22718.t1 | MTR_1g057650 | 53.617 | 235 | 105 | 2 | 1 | 232 | 1 | 234 | 5.09e-88 | 266 |
| MS.gene22718.t1 | MTR_5g076250 | 49.225 | 258 | 117 | 4 | 1 | 251 | 1 | 251 | 1.24e-75 | 234 |
| MS.gene22718.t1 | MTR_7g117745 | 100.000 | 109 | 0 | 0 | 246 | 354 | 2 | 110 | 6.67e-74 | 224 |
| MS.gene22718.t1 | MTR_3g064020 | 44.351 | 239 | 110 | 5 | 1 | 226 | 1 | 229 | 6.08e-61 | 196 |
| MS.gene22718.t1 | MTR_1g066810 | 77.778 | 108 | 24 | 0 | 247 | 354 | 3 | 110 | 4.76e-49 | 160 |
| MS.gene22718.t1 | MTR_3g063990 | 36.576 | 257 | 113 | 5 | 8 | 222 | 2 | 250 | 6.05e-45 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22718.t1 | AT1G01225 | 75.424 | 236 | 57 | 1 | 1 | 236 | 1 | 235 | 5.53e-135 | 385 |
| MS.gene22718.t1 | AT4G00905 | 71.255 | 247 | 62 | 1 | 1 | 238 | 1 | 247 | 5.05e-130 | 372 |
| MS.gene22718.t1 | AT5G06370 | 53.527 | 241 | 104 | 3 | 1 | 236 | 1 | 238 | 9.19e-85 | 257 |
| MS.gene22718.t1 | AT3G02700 | 50.787 | 254 | 111 | 6 | 1 | 248 | 1 | 246 | 4.85e-76 | 235 |
| MS.gene22718.t1 | AT5G16360 | 41.912 | 272 | 122 | 6 | 1 | 237 | 1 | 271 | 2.34e-62 | 201 |
| MS.gene22718.t1 | AT2G35795 | 78.378 | 111 | 22 | 1 | 246 | 354 | 2 | 112 | 1.52e-58 | 185 |
| MS.gene22718.t1 | AT5G16330 | 43.750 | 240 | 107 | 5 | 1 | 232 | 9 | 228 | 8.49e-57 | 185 |
| MS.gene22718.t1 | AT5G03030 | 71.200 | 125 | 34 | 1 | 232 | 354 | 28 | 152 | 1.14e-50 | 166 |
| MS.gene22718.t1 | AT3G09700 | 79.787 | 94 | 17 | 1 | 263 | 354 | 19 | 112 | 8.62e-50 | 162 |
| MS.gene22718.t1 | AT5G03030 | 77.477 | 111 | 23 | 1 | 246 | 354 | 2 | 112 | 1.28e-49 | 162 |
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAATGTATCTTCTTCAAAA+TGG | 0.253229 | 7.4:+762997 | MS.gene22718:CDS |
| GGTCAAGCTTCTTCTGTTAT+TGG | 0.264756 | 7.4:+763120 | MS.gene22718:CDS |
| TATCTTCTTCAAAATGGTTT+TGG | 0.282564 | 7.4:+763003 | MS.gene22718:CDS |
| GCTCGATGGATAACAGTTTC+AGG | 0.291481 | 7.4:-762976 | None:intergenic |
| CCTTTCCTGATTGTGGGTTC+AGG | 0.306141 | 7.4:+762809 | MS.gene22718:CDS |
| ACAGCAATCCCAGCAATTAA+AGG | 0.317983 | 7.4:-766171 | None:intergenic |
| ATTGACTTAAAGTTTCTATC+AGG | 0.325786 | 7.4:-762736 | None:intergenic |
| GTCCCACCTTTCCTGATTGT+GGG | 0.332185 | 7.4:+762803 | MS.gene22718:CDS |
| TTTGCAATGTACTGCAAAAC+TGG | 0.342481 | 7.4:+763060 | MS.gene22718:CDS |
| CAGGCTTCACCTTTAATTGC+TGG | 0.363751 | 7.4:+766162 | MS.gene22718:intron |
| TGTCCCACCTTTCCTGATTG+TGG | 0.372248 | 7.4:+762802 | MS.gene22718:CDS |
| CAGCTCTTATACTTGGTGTT+AGG | 0.376066 | 7.4:+766316 | MS.gene22718:CDS |
| GCAGCATATGCTGGTAAATA+TGG | 0.379014 | 7.4:+766201 | MS.gene22718:CDS |
| AGAAGACTTGGCTGTGAACT+TGG | 0.382220 | 7.4:+763290 | MS.gene22718:CDS |
| CTTTGAATGCTTGCCAAGCC+TGG | 0.382829 | 7.4:-766227 | None:intergenic |
| GAAGAAAGCATTGCAGCTAA+TGG | 0.389297 | 7.4:-763147 | None:intergenic |
| TAATGGCTGCCACCAGCATC+TGG | 0.390187 | 7.4:-768491 | None:intergenic |
| GAAGTGATGTGGTCAAGGTT+GGG | 0.403253 | 7.4:+763265 | MS.gene22718:CDS |
| GAAGCAAAAGATATGATGAT+TGG | 0.421343 | 7.4:+768533 | MS.gene22718:CDS |
| GTGGCTGCTGCAGCATATGC+TGG | 0.421398 | 7.4:+766192 | MS.gene22718:CDS |
| GCTTCTTTAATCTTATCTGT+TGG | 0.444179 | 7.4:-768440 | None:intergenic |
| ATTGTAGACAAGCAAGGAGT+TGG | 0.458301 | 7.4:+763090 | MS.gene22718:CDS |
| AAGTGATGTGGTCAAGGTTG+GGG | 0.470724 | 7.4:+763266 | MS.gene22718:CDS |
| GGTTGCCTGAACCCACAATC+AGG | 0.472264 | 7.4:-762814 | None:intergenic |
| AGGCTTCACCTTTAATTGCT+GGG | 0.473780 | 7.4:+766163 | MS.gene22718:intron |
| GAAAGAACTACTCCACTATT+CGG | 0.477232 | 7.4:-762835 | None:intergenic |
| TCATAAAATTTACGCAATGC+AGG | 0.486334 | 7.4:-766258 | None:intergenic |
| AGAAGTGATGTGGTCAAGGT+TGG | 0.486475 | 7.4:+763264 | MS.gene22718:CDS |
| CAATCAGGAAAGGTGGGACA+AGG | 0.488425 | 7.4:-762799 | None:intergenic |
| TGCTGGTAAATATGGTATCC+AGG | 0.489099 | 7.4:+766209 | MS.gene22718:CDS |
| AAATGCTGATCCACCACCCT+TGG | 0.491232 | 7.4:-768561 | None:intergenic |
| GTGGGACAAGGGGTTGGTGT+TGG | 0.491703 | 7.4:-762787 | None:intergenic |
| CAAGGTTGGGGTAGAAGACT+TGG | 0.494212 | 7.4:+763278 | MS.gene22718:CDS |
| GCTTCCCTCTTCGTCATAGT+AGG | 0.496100 | 7.4:-766294 | None:intergenic |
| GGGTTCAGGCAACCGAATAG+TGG | 0.509445 | 7.4:+762823 | MS.gene22718:CDS |
| ATAACAGTTTCAGGTGGGTC+GGG | 0.514072 | 7.4:-762967 | None:intergenic |
| TTAGACTGCTTCCTTCGAAA+CGG | 0.516654 | 7.4:+762862 | MS.gene22718:CDS |
| GTAAATATGGTATCCAGGCT+TGG | 0.516960 | 7.4:+766214 | MS.gene22718:CDS |
| AAGCAAGGAGTTGGAAGAAG+TGG | 0.518050 | 7.4:+763099 | MS.gene22718:CDS |
| GCACGTGCCACCTCTTATCC+TGG | 0.523495 | 7.4:-762932 | None:intergenic |
| ATTGGAAAGACCAAGGGTGG+TGG | 0.525296 | 7.4:+768551 | MS.gene22718:CDS |
| AGCTTGACCGTGATGAGAAT+AGG | 0.526450 | 7.4:-761725 | None:intergenic |
| ATAGGAGAAAACAGCTCTGT+AGG | 0.528737 | 7.4:-761707 | None:intergenic |
| GGAAAGGTGGGACAAGGGGT+TGG | 0.535853 | 7.4:-762793 | None:intergenic |
| GACTTGTTCCGATGAGGAGG+AGG | 0.537304 | 7.4:+763317 | MS.gene22718:CDS |
| GTTACCGTAGCCATACCAAC+AGG | 0.545756 | 7.4:-763195 | None:intergenic |
| TTGTTAATGCCGAGTCCTGT+TGG | 0.549441 | 7.4:+763180 | MS.gene22718:CDS |
| GATAACAGTTTCAGGTGGGT+CGG | 0.549630 | 7.4:-762968 | None:intergenic |
| AGGGAAGCAGCTCTTATACT+TGG | 0.552024 | 7.4:+766309 | MS.gene22718:CDS |
| AGTAGATATGCAACTGACAT+TGG | 0.566026 | 7.4:+763237 | MS.gene22718:CDS |
| GTAGGTGTAGACATGATCAC+CGG | 0.568352 | 7.4:-761689 | None:intergenic |
| ACTTGGCTGTGAACTTGGGA+TGG | 0.569461 | 7.4:+763295 | MS.gene22718:CDS |
| GAGAGACACGACATCAAACC+CGG | 0.570178 | 7.4:+761670 | MS.gene22718:CDS |
| TTTAATTGCTGGGATTGCTG+TGG | 0.571174 | 7.4:+766173 | MS.gene22718:CDS |
| GTTGCAAACCATCCAGATGC+TGG | 0.574874 | 7.4:+768479 | MS.gene22718:CDS |
| AATGCCGAGTCCTGTTGGTA+TGG | 0.579949 | 7.4:+763185 | MS.gene22718:CDS |
| GGCTGCCACCAGCATCTGGA+TGG | 0.580035 | 7.4:-768487 | None:intergenic |
| ATCAGGAAAGGTGGGACAAG+GGG | 0.583759 | 7.4:-762797 | None:intergenic |
| AGTAAAGAGAACCGTTTCGA+AGG | 0.585696 | 7.4:-762873 | None:intergenic |
| TCCAACTACCTCCTCCTCAT+CGG | 0.586315 | 7.4:-763325 | None:intergenic |
| CATTAGAAGTGATGTGGTCA+AGG | 0.589386 | 7.4:+763260 | MS.gene22718:CDS |
| ATGGACTTGTTCCGATGAGG+AGG | 0.591554 | 7.4:+763314 | MS.gene22718:CDS |
| AATCAGGAAAGGTGGGACAA+GGG | 0.592653 | 7.4:-762798 | None:intergenic |
| GAGTCCTGTTGGTATGGCTA+CGG | 0.594150 | 7.4:+763191 | MS.gene22718:CDS |
| CGATGGATAACAGTTTCAGG+TGG | 0.599166 | 7.4:-762973 | None:intergenic |
| GAAGACTTGGCTGTGAACTT+GGG | 0.599819 | 7.4:+763291 | MS.gene22718:CDS |
| GAACCCACAATCAGGAAAGG+TGG | 0.600274 | 7.4:-762806 | None:intergenic |
| GGTATGGCTACGGTAACAGC+AGG | 0.600551 | 7.4:+763201 | MS.gene22718:CDS |
| AACCCACAATCAGGAAAGGT+GGG | 0.602727 | 7.4:-762805 | None:intergenic |
| GAAGAAGATACATTGCTCGA+TGG | 0.604177 | 7.4:-762990 | None:intergenic |
| AGAAGCACATAGGAGAGTGA+TGG | 0.627347 | 7.4:+768457 | MS.gene22718:CDS |
| TATGATGATTGGAAAGACCA+AGG | 0.628338 | 7.4:+768544 | MS.gene22718:CDS |
| TCAGCCTACTATGACGAAGA+GGG | 0.630053 | 7.4:+766290 | MS.gene22718:CDS |
| TTCAGCCTACTATGACGAAG+AGG | 0.630974 | 7.4:+766289 | MS.gene22718:CDS |
| CTCGGCATTAACAACTTGAG+AGG | 0.635148 | 7.4:-763171 | None:intergenic |
| CCTGAACCCACAATCAGGAA+AGG | 0.637605 | 7.4:-762809 | None:intergenic |
| TAAAATTTACGCAATGCAGG+TGG | 0.638402 | 7.4:-766255 | None:intergenic |
| TTTCTTACCAGGATAAGAGG+TGG | 0.640216 | 7.4:+762925 | MS.gene22718:CDS |
| ATAAGATTAAAGAAGCACAT+AGG | 0.640913 | 7.4:+768447 | MS.gene22718:CDS |
| ATGATGATTGGAAAGACCAA+GGG | 0.641235 | 7.4:+768545 | MS.gene22718:CDS |
| GGGATGGACTTGTTCCGATG+AGG | 0.647617 | 7.4:+763311 | MS.gene22718:CDS |
| GATGGATAACAGTTTCAGGT+GGG | 0.675658 | 7.4:-762972 | None:intergenic |
| GTAGCCATACCAACAGGACT+CGG | 0.681475 | 7.4:-763189 | None:intergenic |
| TAGGTGTAGACATGATCACC+GGG | 0.692039 | 7.4:-761688 | None:intergenic |
| ATGATTGGAAAGACCAAGGG+TGG | 0.710004 | 7.4:+768548 | MS.gene22718:CDS |
| GCAAACCATCCAGATGCTGG+TGG | 0.711252 | 7.4:+768482 | MS.gene22718:CDS |
| CATTGGCATTAGAAGTGATG+TGG | 0.712623 | 7.4:+763254 | MS.gene22718:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 761646 | 768586 | 761646 | ID=MS.gene22718 |
| chr7.4 | mRNA | 761646 | 768586 | 761646 | ID=MS.gene22718.t1;Parent=MS.gene22718 |
| chr7.4 | exon | 761646 | 761739 | 761646 | ID=MS.gene22718.t1.exon1;Parent=MS.gene22718.t1 |
| chr7.4 | CDS | 761646 | 761739 | 761646 | ID=cds.MS.gene22718.t1;Parent=MS.gene22718.t1 |
| chr7.4 | exon | 762698 | 763338 | 762698 | ID=MS.gene22718.t1.exon2;Parent=MS.gene22718.t1 |
| chr7.4 | CDS | 762698 | 763338 | 762698 | ID=cds.MS.gene22718.t1;Parent=MS.gene22718.t1 |
| chr7.4 | exon | 766165 | 766337 | 766165 | ID=MS.gene22718.t1.exon3;Parent=MS.gene22718.t1 |
| chr7.4 | CDS | 766165 | 766337 | 766165 | ID=cds.MS.gene22718.t1;Parent=MS.gene22718.t1 |
| chr7.4 | exon | 768430 | 768586 | 768430 | ID=MS.gene22718.t1.exon4;Parent=MS.gene22718.t1 |
| chr7.4 | CDS | 768430 | 768586 | 768430 | ID=cds.MS.gene22718.t1;Parent=MS.gene22718.t1 |
| Gene Sequence |
| Protein sequence |
