Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050667.t1 | XP_013450668.1 | 98 | 246 | 4 | 1 | 1 | 245 | 1 | 246 | 2.90E-139 | 505 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050667.t1 | Q8RV04 | 78.4 | 111 | 22 | 1 | 246 | 354 | 2 | 112 | 1.1e-43 | 178.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050667.t1 | I3SHV8 | 98.0 | 246 | 4 | 1 | 1 | 245 | 1 | 246 | 2.1e-139 | 505.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050667 | MS.gene052169 | 0.802932 | 4.44E-49 | -1.69E-46 |
| MS.gene050667 | MS.gene057990 | 0.805237 | 1.47E-49 | -1.69E-46 |
| MS.gene050667 | MS.gene058535 | 0.801013 | 1.10E-48 | -1.69E-46 |
| MS.gene050667 | MS.gene063489 | 0.859461 | 4.07E-63 | -1.69E-46 |
| MS.gene050667 | MS.gene070324 | 0.826544 | 2.56E-54 | -1.69E-46 |
| MS.gene050667 | MS.gene071422 | 0.811763 | 5.95E-51 | -1.69E-46 |
| MS.gene050667 | MS.gene073059 | 0.815067 | 1.12E-51 | -1.69E-46 |
| MS.gene050667 | MS.gene073062 | 0.811733 | 6.04E-51 | -1.69E-46 |
| MS.gene050667 | MS.gene074377 | 0.850859 | 1.29E-60 | -1.69E-46 |
| MS.gene050667 | MS.gene09356 | 0.832095 | 1.15E-55 | -1.69E-46 |
| MS.gene050667 | MS.gene22718 | 0.973038 | 9.35E-136 | -1.69E-46 |
| MS.gene050667 | MS.gene25932 | 0.853455 | 2.36E-61 | -1.69E-46 |
| MS.gene050667 | MS.gene42070 | 0.802105 | 6.57E-49 | -1.69E-46 |
| MS.gene050667 | MS.gene46636 | 0.833631 | 4.77E-56 | -1.69E-46 |
| MS.gene050667 | MS.gene50925 | 0.801243 | 9.87E-49 | -1.69E-46 |
| MS.gene050667 | MS.gene54373 | 0.800045 | 1.73E-48 | -1.69E-46 |
| MS.gene050667 | MS.gene57734 | 0.87102 | 9.39E-67 | -1.69E-46 |
| MS.gene050667 | MS.gene58738 | 0.860731 | 1.68E-63 | -1.69E-46 |
| MS.gene050667 | MS.gene58838 | 0.819325 | 1.23E-52 | -1.69E-46 |
| MS.gene050667 | MS.gene60760 | 0.802063 | 6.70E-49 | -1.69E-46 |
| MS.gene050667 | MS.gene65695 | 0.801599 | 8.35E-49 | -1.69E-46 |
| MS.gene050667 | MS.gene69797 | 0.80875 | 2.66E-50 | -1.69E-46 |
| MS.gene050667 | MS.gene70604 | 0.853883 | 1.78E-61 | -1.69E-46 |
| MS.gene050667 | MS.gene72164 | 0.82715 | 1.83E-54 | -1.69E-46 |
| MS.gene050667 | MS.gene75761 | 0.805388 | 1.37E-49 | -1.69E-46 |
| MS.gene050667 | MS.gene75915 | 0.824335 | 8.54E-54 | -1.69E-46 |
| MS.gene050667 | MS.gene75918 | 0.811922 | 5.49E-51 | -1.69E-46 |
| MS.gene050667 | MS.gene77904 | 0.8186 | 1.80E-52 | -1.69E-46 |
| MS.gene050667 | MS.gene88616 | 0.820123 | 8.10E-53 | -1.69E-46 |
| MS.gene050667 | MS.gene89546 | 0.807861 | 4.11E-50 | -1.69E-46 |
| MS.gene050667 | MS.gene91133 | 0.812786 | 3.56E-51 | -1.69E-46 |
| MS.gene050667 | MS.gene91174 | 0.815925 | 7.20E-52 | -1.69E-46 |
| MS.gene050667 | MS.gene91871 | 0.80963 | 1.72E-50 | -1.69E-46 |
| MS.gene050667 | MS.gene96186 | 0.803078 | 4.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050667.t1 | MTR_7g117750 | 97.571 | 247 | 5 | 1 | 1 | 246 | 1 | 247 | 0.0 | 507 |
| MS.gene050667.t1 | MTR_1g057650 | 53.617 | 235 | 105 | 2 | 1 | 232 | 1 | 234 | 5.09e-88 | 266 |
| MS.gene050667.t1 | MTR_5g076250 | 49.225 | 258 | 117 | 4 | 1 | 251 | 1 | 251 | 1.24e-75 | 234 |
| MS.gene050667.t1 | MTR_7g117745 | 100.000 | 109 | 0 | 0 | 246 | 354 | 2 | 110 | 6.67e-74 | 224 |
| MS.gene050667.t1 | MTR_3g064020 | 44.351 | 239 | 110 | 5 | 1 | 226 | 1 | 229 | 6.08e-61 | 196 |
| MS.gene050667.t1 | MTR_1g066810 | 77.778 | 108 | 24 | 0 | 247 | 354 | 3 | 110 | 4.76e-49 | 160 |
| MS.gene050667.t1 | MTR_3g063990 | 36.576 | 257 | 113 | 5 | 8 | 222 | 2 | 250 | 6.05e-45 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050667.t1 | AT1G01225 | 75.424 | 236 | 57 | 1 | 1 | 236 | 1 | 235 | 5.53e-135 | 385 |
| MS.gene050667.t1 | AT4G00905 | 71.255 | 247 | 62 | 1 | 1 | 238 | 1 | 247 | 5.05e-130 | 372 |
| MS.gene050667.t1 | AT5G06370 | 53.527 | 241 | 104 | 3 | 1 | 236 | 1 | 238 | 9.19e-85 | 257 |
| MS.gene050667.t1 | AT3G02700 | 50.787 | 254 | 111 | 6 | 1 | 248 | 1 | 246 | 4.85e-76 | 235 |
| MS.gene050667.t1 | AT5G16360 | 41.912 | 272 | 122 | 6 | 1 | 237 | 1 | 271 | 2.34e-62 | 201 |
| MS.gene050667.t1 | AT2G35795 | 78.378 | 111 | 22 | 1 | 246 | 354 | 2 | 112 | 1.52e-58 | 185 |
| MS.gene050667.t1 | AT5G16330 | 43.750 | 240 | 107 | 5 | 1 | 232 | 9 | 228 | 8.49e-57 | 185 |
| MS.gene050667.t1 | AT5G03030 | 71.200 | 125 | 34 | 1 | 232 | 354 | 28 | 152 | 1.14e-50 | 166 |
| MS.gene050667.t1 | AT3G09700 | 79.787 | 94 | 17 | 1 | 263 | 354 | 19 | 112 | 8.62e-50 | 162 |
| MS.gene050667.t1 | AT5G03030 | 77.477 | 111 | 23 | 1 | 246 | 354 | 2 | 112 | 1.28e-49 | 162 |
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAATGTATCTTCTTCAAAA+TGG | 0.253229 | 7.3:+711831 | MS.gene050667:CDS |
| GGTCAAGCTTCTTCTGTTAT+TGG | 0.264756 | 7.3:+711954 | MS.gene050667:CDS |
| TATCTTCTTCAAAATGGTTT+TGG | 0.282564 | 7.3:+711837 | MS.gene050667:CDS |
| GCTCGATGGATAACAGTTTC+AGG | 0.291481 | 7.3:-711810 | None:intergenic |
| CCTTTCCTGATTGTGGGTTC+AGG | 0.306141 | 7.3:+711643 | MS.gene050667:CDS |
| ACAGCAATCCCAGCAATTAA+AGG | 0.317983 | 7.3:-714991 | None:intergenic |
| ATTGACTTAAAGTTTCTATC+AGG | 0.325786 | 7.3:-711570 | None:intergenic |
| GTCCCACCTTTCCTGATTGT+GGG | 0.332185 | 7.3:+711637 | MS.gene050667:CDS |
| TTTGCAATGTACTGCAAAAC+TGG | 0.342481 | 7.3:+711894 | MS.gene050667:CDS |
| CAGGCTTCACCTTTAATTGC+TGG | 0.363751 | 7.3:+714982 | MS.gene050667:intron |
| TGTCCCACCTTTCCTGATTG+TGG | 0.372248 | 7.3:+711636 | MS.gene050667:CDS |
| CAGCTCTTATACTTGGTGTT+AGG | 0.376066 | 7.3:+715136 | MS.gene050667:CDS |
| GCAGCATATGCTGGTAAATA+TGG | 0.379014 | 7.3:+715021 | MS.gene050667:CDS |
| AGAAGACTTGGCTGTGAACT+TGG | 0.382220 | 7.3:+712124 | MS.gene050667:CDS |
| CTTTGAATGCTTGCCAAGCC+TGG | 0.382829 | 7.3:-715047 | None:intergenic |
| GAAGAAAGCATTGCAGCTAA+TGG | 0.389297 | 7.3:-711981 | None:intergenic |
| TAATGGCTGCCACCAGCATC+TGG | 0.390187 | 7.3:-717359 | None:intergenic |
| GAAGTGATGTGGTCAAGGTT+GGG | 0.403253 | 7.3:+712099 | MS.gene050667:CDS |
| GAAGCAAAAGATATGATGAT+TGG | 0.421343 | 7.3:+717401 | MS.gene050667:CDS |
| GTGGCTGCTGCAGCATATGC+TGG | 0.421398 | 7.3:+715012 | MS.gene050667:CDS |
| GCTTCTTTAATCTTATCTGT+TGG | 0.444179 | 7.3:-717308 | None:intergenic |
| ATTGTAGACAAGCAAGGAGT+TGG | 0.458301 | 7.3:+711924 | MS.gene050667:CDS |
| AAGTGATGTGGTCAAGGTTG+GGG | 0.470724 | 7.3:+712100 | MS.gene050667:CDS |
| GGTTGCCTGAACCCACAATC+AGG | 0.472264 | 7.3:-711648 | None:intergenic |
| AGGCTTCACCTTTAATTGCT+GGG | 0.473780 | 7.3:+714983 | MS.gene050667:intron |
| GAAAGAACTACTCCACTATT+CGG | 0.477232 | 7.3:-711669 | None:intergenic |
| TCATAAAATTTACGCAATGC+AGG | 0.486334 | 7.3:-715078 | None:intergenic |
| AGAAGTGATGTGGTCAAGGT+TGG | 0.486475 | 7.3:+712098 | MS.gene050667:CDS |
| CAATCAGGAAAGGTGGGACA+AGG | 0.488425 | 7.3:-711633 | None:intergenic |
| TGCTGGTAAATATGGTATCC+AGG | 0.489099 | 7.3:+715029 | MS.gene050667:CDS |
| AAATGCTGATCCACCACCCT+TGG | 0.491232 | 7.3:-717429 | None:intergenic |
| GTGGGACAAGGGGTTGGTGT+TGG | 0.491703 | 7.3:-711621 | None:intergenic |
| CAAGGTTGGGGTAGAAGACT+TGG | 0.494212 | 7.3:+712112 | MS.gene050667:CDS |
| GCTTCCCTCTTCGTCATAGT+AGG | 0.496100 | 7.3:-715114 | None:intergenic |
| GGGTTCAGGCAACCGAATAG+TGG | 0.509445 | 7.3:+711657 | MS.gene050667:CDS |
| ATAACAGTTTCAGGTGGGTC+GGG | 0.514072 | 7.3:-711801 | None:intergenic |
| TTAGACTGCTTCCTTCGAAA+CGG | 0.516654 | 7.3:+711696 | MS.gene050667:CDS |
| GTAAATATGGTATCCAGGCT+TGG | 0.516960 | 7.3:+715034 | MS.gene050667:CDS |
| AAGCAAGGAGTTGGAAGAAG+TGG | 0.518050 | 7.3:+711933 | MS.gene050667:CDS |
| GCACGTGCCACCTCTTATCC+TGG | 0.523495 | 7.3:-711766 | None:intergenic |
| ATTGGAAAGACCAAGGGTGG+TGG | 0.525296 | 7.3:+717419 | MS.gene050667:CDS |
| AGCTTGACCGTGATGAGAAT+AGG | 0.526450 | 7.3:-710535 | None:intergenic |
| ATAGGAGAAAACAGCTCTGT+AGG | 0.528737 | 7.3:-710517 | None:intergenic |
| GGAAAGGTGGGACAAGGGGT+TGG | 0.535853 | 7.3:-711627 | None:intergenic |
| GACTTGTTCCGATGAGGAGG+AGG | 0.537304 | 7.3:+712151 | MS.gene050667:CDS |
| GTTACCGTAGCCATACCAAC+AGG | 0.545756 | 7.3:-712029 | None:intergenic |
| TTGTTAATGCCGAGTCCTGT+TGG | 0.549441 | 7.3:+712014 | MS.gene050667:CDS |
| GATAACAGTTTCAGGTGGGT+CGG | 0.549630 | 7.3:-711802 | None:intergenic |
| AGGGAAGCAGCTCTTATACT+TGG | 0.552024 | 7.3:+715129 | MS.gene050667:CDS |
| AGTAGATATGCAACTGACAT+TGG | 0.566026 | 7.3:+712071 | MS.gene050667:CDS |
| GTAGGTGTAGACATGATCAC+CGG | 0.568352 | 7.3:-710499 | None:intergenic |
| ACTTGGCTGTGAACTTGGGA+TGG | 0.569461 | 7.3:+712129 | MS.gene050667:CDS |
| GAGAGACACGACATCAAACC+CGG | 0.570178 | 7.3:+710480 | MS.gene050667:CDS |
| TTTAATTGCTGGGATTGCTG+TGG | 0.571174 | 7.3:+714993 | MS.gene050667:CDS |
| GTTGCAAACCATCCAGATGC+TGG | 0.574874 | 7.3:+717347 | MS.gene050667:CDS |
| AATGCCGAGTCCTGTTGGTA+TGG | 0.579949 | 7.3:+712019 | MS.gene050667:CDS |
| GGCTGCCACCAGCATCTGGA+TGG | 0.580035 | 7.3:-717355 | None:intergenic |
| ATCAGGAAAGGTGGGACAAG+GGG | 0.583759 | 7.3:-711631 | None:intergenic |
| AGTAAAGAGAACCGTTTCGA+AGG | 0.585696 | 7.3:-711707 | None:intergenic |
| TCCAACTACCTCCTCCTCAT+CGG | 0.586315 | 7.3:-712159 | None:intergenic |
| CATTAGAAGTGATGTGGTCA+AGG | 0.589386 | 7.3:+712094 | MS.gene050667:CDS |
| ATGGACTTGTTCCGATGAGG+AGG | 0.591554 | 7.3:+712148 | MS.gene050667:CDS |
| AATCAGGAAAGGTGGGACAA+GGG | 0.592653 | 7.3:-711632 | None:intergenic |
| GAGTCCTGTTGGTATGGCTA+CGG | 0.594150 | 7.3:+712025 | MS.gene050667:CDS |
| CGATGGATAACAGTTTCAGG+TGG | 0.599166 | 7.3:-711807 | None:intergenic |
| GAAGACTTGGCTGTGAACTT+GGG | 0.599819 | 7.3:+712125 | MS.gene050667:CDS |
| GAACCCACAATCAGGAAAGG+TGG | 0.600274 | 7.3:-711640 | None:intergenic |
| GGTATGGCTACGGTAACAGC+AGG | 0.600551 | 7.3:+712035 | MS.gene050667:CDS |
| AACCCACAATCAGGAAAGGT+GGG | 0.602727 | 7.3:-711639 | None:intergenic |
| GAAGAAGATACATTGCTCGA+TGG | 0.604177 | 7.3:-711824 | None:intergenic |
| AGAAGCACATAGGAGAGTGA+TGG | 0.627347 | 7.3:+717325 | MS.gene050667:CDS |
| TATGATGATTGGAAAGACCA+AGG | 0.628338 | 7.3:+717412 | MS.gene050667:CDS |
| TCAGCCTACTATGACGAAGA+GGG | 0.630053 | 7.3:+715110 | MS.gene050667:CDS |
| TTCAGCCTACTATGACGAAG+AGG | 0.630974 | 7.3:+715109 | MS.gene050667:CDS |
| CTCGGCATTAACAACTTGAG+AGG | 0.635148 | 7.3:-712005 | None:intergenic |
| CCTGAACCCACAATCAGGAA+AGG | 0.637605 | 7.3:-711643 | None:intergenic |
| TAAAATTTACGCAATGCAGG+TGG | 0.638402 | 7.3:-715075 | None:intergenic |
| TTTCTTACCAGGATAAGAGG+TGG | 0.640216 | 7.3:+711759 | MS.gene050667:CDS |
| ATAAGATTAAAGAAGCACAT+AGG | 0.640913 | 7.3:+717315 | MS.gene050667:CDS |
| ATGATGATTGGAAAGACCAA+GGG | 0.641235 | 7.3:+717413 | MS.gene050667:CDS |
| GGGATGGACTTGTTCCGATG+AGG | 0.647617 | 7.3:+712145 | MS.gene050667:CDS |
| GATGGATAACAGTTTCAGGT+GGG | 0.675658 | 7.3:-711806 | None:intergenic |
| GTAGCCATACCAACAGGACT+CGG | 0.681475 | 7.3:-712023 | None:intergenic |
| TAGGTGTAGACATGATCACC+GGG | 0.692039 | 7.3:-710498 | None:intergenic |
| ATGATTGGAAAGACCAAGGG+TGG | 0.710004 | 7.3:+717416 | MS.gene050667:CDS |
| GCAAACCATCCAGATGCTGG+TGG | 0.711252 | 7.3:+717350 | MS.gene050667:CDS |
| CATTGGCATTAGAAGTGATG+TGG | 0.712623 | 7.3:+712088 | MS.gene050667:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 710456 | 717454 | 710456 | ID=MS.gene050667 |
| chr7.3 | mRNA | 710456 | 717454 | 710456 | ID=MS.gene050667.t1;Parent=MS.gene050667 |
| chr7.3 | exon | 710456 | 710549 | 710456 | ID=MS.gene050667.t1.exon1;Parent=MS.gene050667.t1 |
| chr7.3 | CDS | 710456 | 710549 | 710456 | ID=cds.MS.gene050667.t1;Parent=MS.gene050667.t1 |
| chr7.3 | exon | 711532 | 712172 | 711532 | ID=MS.gene050667.t1.exon2;Parent=MS.gene050667.t1 |
| chr7.3 | CDS | 711532 | 712172 | 711532 | ID=cds.MS.gene050667.t1;Parent=MS.gene050667.t1 |
| chr7.3 | exon | 714985 | 715157 | 714985 | ID=MS.gene050667.t1.exon3;Parent=MS.gene050667.t1 |
| chr7.3 | CDS | 714985 | 715157 | 714985 | ID=cds.MS.gene050667.t1;Parent=MS.gene050667.t1 |
| chr7.3 | exon | 717298 | 717454 | 717298 | ID=MS.gene050667.t1.exon4;Parent=MS.gene050667.t1 |
| chr7.3 | CDS | 717298 | 717454 | 717298 | ID=cds.MS.gene050667.t1;Parent=MS.gene050667.t1 |
| Gene Sequence |
| Protein sequence |
