Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22736.t1 | AES82811.1 | 95.2 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 5.70E-48 | 199.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22736.t1 | G7L533 | 95.2 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 4.1e-48 | 199.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050685 | MS.gene22736 | 0.848791 | 4.89E-60 | -1.69E-46 |
| MS.gene057754 | MS.gene22736 | 0.809051 | 2.29E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22736.t1 | MTR_7g118070 | 95.192 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 1.86e-68 | 200 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAAAGGGGCTGAGTTTA+AGG | 0.194747 | 7.4:+613553 | MS.gene22736:CDS |
| GGCCAACTCAAGGATTTAAT+AGG | 0.281462 | 7.4:+613405 | MS.gene22736:CDS |
| AACAATGACAATTTAGAATT+AGG | 0.285252 | 7.4:+613441 | MS.gene22736:CDS |
| CTGTGGTAGTTCACCGCATT+CGG | 0.308570 | 7.4:+613346 | MS.gene22736:CDS |
| TCTTCGCTATGTTCTTGAAT+TGG | 0.381899 | 7.4:-613576 | None:intergenic |
| TAAATCCTTGAGTTGGCCTT+TGG | 0.387136 | 7.4:-613400 | None:intergenic |
| AAAATGGGTAATGCTTGCTT+TGG | 0.394791 | 7.4:+613291 | None:intergenic |
| GCCAACTCAAGGATTTAATA+GGG | 0.437318 | 7.4:+613406 | MS.gene22736:CDS |
| TGTGGTAGTTCACCGCATTC+GGG | 0.441159 | 7.4:+613347 | MS.gene22736:CDS |
| ACGAGCACGAAGCCTCCCTT+TGG | 0.474923 | 7.4:-613487 | None:intergenic |
| TCCCTATTAAATCCTTGAGT+TGG | 0.476466 | 7.4:-613407 | None:intergenic |
| AGCATTCTTGAACGATCAAA+TGG | 0.501702 | 7.4:-613464 | None:intergenic |
| GGATCAAGCCGTGACGATCA+AGG | 0.532320 | 7.4:+613368 | MS.gene22736:CDS |
| CGAATGCGGTGAACTACCAC+AGG | 0.532604 | 7.4:-613345 | None:intergenic |
| ATCGTTCAAGAATGCTCCAA+AGG | 0.558651 | 7.4:+613471 | MS.gene22736:CDS |
| CGTGTTGTAACTGCTGCAGT+TGG | 0.558826 | 7.4:+613507 | MS.gene22736:CDS |
| CAAGGATTTAATAGGGAAGG+TGG | 0.561353 | 7.4:+613413 | MS.gene22736:CDS |
| TCGTTCAAGAATGCTCCAAA+GGG | 0.578470 | 7.4:+613472 | MS.gene22736:CDS |
| CATTCTCACCTTGATCGTCA+CGG | 0.582038 | 7.4:-613376 | None:intergenic |
| GAAGATGAGCAACAAGGGAG+TGG | 0.588540 | 7.4:+613317 | MS.gene22736:CDS |
| GGAAAGAAGATGAGCAACAA+GGG | 0.598826 | 7.4:+613312 | MS.gene22736:CDS |
| TGGAAAGAAGATGAGCAACA+AGG | 0.599291 | 7.4:+613311 | MS.gene22736:CDS |
| AGAACATAGCGAAGATGTGT+AGG | 0.601986 | 7.4:+613584 | MS.gene22736:CDS |
| AATGACCAAAGGCCAACTCA+AGG | 0.609242 | 7.4:+613395 | MS.gene22736:CDS |
| TTCAAGAATGCTCCAAAGGG+AGG | 0.614086 | 7.4:+613475 | MS.gene22736:CDS |
| ACTCAAGGATTTAATAGGGA+AGG | 0.618810 | 7.4:+613410 | MS.gene22736:CDS |
| TCACGGCTTGATCCCGAATG+CGG | 0.664295 | 7.4:-613359 | None:intergenic |
| CAAGGGAGTGGTGCACCCTG+TGG | 0.671108 | 7.4:+613329 | MS.gene22736:CDS |
| ATCAAGGTGAGAATGACCAA+AGG | 0.688055 | 7.4:+613384 | MS.gene22736:CDS |
| GAATGCGGTGAACTACCACA+GGG | 0.775194 | 7.4:-613344 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAATGACAATTTAGAATT+AGG | + | chr7.4:613441-613460 | MS.gene22736:CDS | 20.0% |
| ! | ATACGTACAAGTTTTCAAAG+GGG | + | chr7.4:613541-613560 | MS.gene22736:CDS | 30.0% |
| ! | CATACGTACAAGTTTTCAAA+GGG | + | chr7.4:613540-613559 | MS.gene22736:CDS | 30.0% |
| ! | TCATACGTACAAGTTTTCAA+AGG | + | chr7.4:613539-613558 | MS.gene22736:CDS | 30.0% |
| ACTCAAGGATTTAATAGGGA+AGG | + | chr7.4:613410-613429 | MS.gene22736:CDS | 35.0% | |
| AGCATTCTTGAACGATCAAA+TGG | - | chr7.4:613467-613486 | None:intergenic | 35.0% | |
| GCCAACTCAAGGATTTAATA+GGG | + | chr7.4:613406-613425 | MS.gene22736:CDS | 35.0% | |
| TCCCTATTAAATCCTTGAGT+TGG | - | chr7.4:613410-613429 | None:intergenic | 35.0% | |
| TCTTCGCTATGTTCTTGAAT+TGG | - | chr7.4:613579-613598 | None:intergenic | 35.0% | |
| ATCAAGGTGAGAATGACCAA+AGG | + | chr7.4:613384-613403 | MS.gene22736:CDS | 40.0% | |
| ATCGTTCAAGAATGCTCCAA+AGG | + | chr7.4:613471-613490 | MS.gene22736:CDS | 40.0% | |
| CAAGGATTTAATAGGGAAGG+TGG | + | chr7.4:613413-613432 | MS.gene22736:CDS | 40.0% | |
| GGAAAGAAGATGAGCAACAA+GGG | + | chr7.4:613312-613331 | MS.gene22736:CDS | 40.0% | |
| GGCCAACTCAAGGATTTAAT+AGG | + | chr7.4:613405-613424 | MS.gene22736:CDS | 40.0% | |
| TCGTTCAAGAATGCTCCAAA+GGG | + | chr7.4:613472-613491 | MS.gene22736:CDS | 40.0% | |
| TGGAAAGAAGATGAGCAACA+AGG | + | chr7.4:613311-613330 | MS.gene22736:CDS | 40.0% | |
| TTTCAAAGGGGCTGAGTTTA+AGG | + | chr7.4:613553-613572 | MS.gene22736:CDS | 40.0% | |
| !! | TAAATCCTTGAGTTGGCCTT+TGG | - | chr7.4:613403-613422 | None:intergenic | 40.0% |
| AATGACCAAAGGCCAACTCA+AGG | + | chr7.4:613395-613414 | MS.gene22736:CDS | 45.0% | |
| CATTCTCACCTTGATCGTCA+CGG | - | chr7.4:613379-613398 | None:intergenic | 45.0% | |
| TTCAAGAATGCTCCAAAGGG+AGG | + | chr7.4:613475-613494 | MS.gene22736:CDS | 45.0% | |
| CGTGTTGTAACTGCTGCAGT+TGG | + | chr7.4:613507-613526 | MS.gene22736:CDS | 50.0% | |
| CTGTGGTAGTTCACCGCATT+CGG | + | chr7.4:613346-613365 | MS.gene22736:CDS | 50.0% | |
| GAAGATGAGCAACAAGGGAG+TGG | + | chr7.4:613317-613336 | MS.gene22736:CDS | 50.0% | |
| GAATGCGGTGAACTACCACA+GGG | - | chr7.4:613347-613366 | None:intergenic | 50.0% | |
| TGTGGTAGTTCACCGCATTC+GGG | + | chr7.4:613347-613366 | MS.gene22736:CDS | 50.0% | |
| CGAATGCGGTGAACTACCAC+AGG | - | chr7.4:613348-613367 | None:intergenic | 55.0% | |
| GGATCAAGCCGTGACGATCA+AGG | + | chr7.4:613368-613387 | MS.gene22736:CDS | 55.0% | |
| TCACGGCTTGATCCCGAATG+CGG | - | chr7.4:613362-613381 | None:intergenic | 55.0% | |
| ACGAGCACGAAGCCTCCCTT+TGG | - | chr7.4:613490-613509 | None:intergenic | 60.0% | |
| CAAGGGAGTGGTGCACCCTG+TGG | + | chr7.4:613329-613348 | MS.gene22736:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 613294 | 613605 | 613294 | ID=MS.gene22736 |
| chr7.4 | mRNA | 613294 | 613605 | 613294 | ID=MS.gene22736.t1;Parent=MS.gene22736 |
| chr7.4 | exon | 613294 | 613605 | 613294 | ID=MS.gene22736.t1.exon1;Parent=MS.gene22736.t1 |
| chr7.4 | CDS | 613294 | 613605 | 613294 | ID=cds.MS.gene22736.t1;Parent=MS.gene22736.t1 |
| Gene Sequence |
| Protein sequence |
