Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050685.t1 | AES82811.1 | 80.7 | 109 | 15 | 2 | 1 | 108 | 1 | 104 | 4.30E-38 | 167.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050685.t1 | G7L533 | 80.7 | 109 | 15 | 2 | 1 | 108 | 1 | 104 | 3.1e-38 | 167.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050128 | MS.gene050685 | 0.84301 | 1.82E-58 | -1.69E-46 |
| MS.gene050685 | MS.gene052178 | 0.80417 | 2.46E-49 | -1.69E-46 |
| MS.gene050685 | MS.gene05316 | 0.802882 | 4.54E-49 | -1.69E-46 |
| MS.gene050685 | MS.gene063103 | 0.82568 | 4.11E-54 | -1.69E-46 |
| MS.gene050685 | MS.gene070491 | 0.803046 | 4.20E-49 | -1.69E-46 |
| MS.gene050685 | MS.gene08691 | 0.844815 | 5.98E-59 | -1.69E-46 |
| MS.gene050685 | MS.gene08753 | 0.837228 | 5.88E-57 | -1.69E-46 |
| MS.gene050685 | MS.gene22736 | 0.848791 | 4.89E-60 | -1.69E-46 |
| MS.gene050685 | MS.gene23887 | 0.825936 | 3.57E-54 | -1.69E-46 |
| MS.gene050685 | MS.gene32736 | 0.806791 | 6.93E-50 | -1.69E-46 |
| MS.gene050685 | MS.gene51304 | 0.811895 | 5.57E-51 | -1.69E-46 |
| MS.gene050685 | MS.gene53556 | 0.803735 | 3.02E-49 | -1.69E-46 |
| MS.gene050685 | MS.gene59149 | 0.807242 | 5.56E-50 | -1.69E-46 |
| MS.gene050685 | MS.gene64994 | 0.812411 | 4.30E-51 | -1.69E-46 |
| MS.gene050685 | MS.gene68151 | 0.822737 | 2.02E-53 | -1.69E-46 |
| MS.gene050685 | MS.gene68751 | 0.823021 | 1.73E-53 | -1.69E-46 |
| MS.gene050685 | MS.gene70101 | 0.805095 | 1.57E-49 | -1.69E-46 |
| MS.gene050685 | MS.gene95539 | 0.806922 | 6.50E-50 | -1.69E-46 |
| MS.gene050685 | MS.gene99874 | 0.800562 | 1.36E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050685.t1 | MTR_7g118070 | 90.826 | 109 | 4 | 2 | 1 | 108 | 1 | 104 | 7.10e-66 | 194 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAAAGGGGCTGAGTTTA+AGG | 0.194747 | 7.4:+527321 | MS.gene050685:CDS |
| GGCCAACTCAAGGATTTAAT+AGG | 0.281462 | 7.4:+527173 | MS.gene050685:CDS |
| AACAATGACAATTTAGAATT+AGG | 0.285252 | 7.4:+527209 | MS.gene050685:CDS |
| CTGTGGTAGTTCACCGCATT+CGG | 0.308570 | 7.4:+527114 | MS.gene050685:CDS |
| TCTTCGCTATGTTCTTGAAT+TGG | 0.381899 | 7.4:-527344 | None:intergenic |
| TAAATCCTTGAGTTGGCCTT+TGG | 0.387136 | 7.4:-527168 | None:intergenic |
| AAAATGGGTAATGCTTGCTT+TGG | 0.394791 | 7.4:+527044 | None:intergenic |
| GCCAACTCAAGGATTTAATA+GGG | 0.437318 | 7.4:+527174 | MS.gene050685:CDS |
| TGTGGTAGTTCACCGCATTC+GGG | 0.441159 | 7.4:+527115 | MS.gene050685:CDS |
| ACGAGCACGAAGCCTCCCTT+TGG | 0.474923 | 7.4:-527255 | None:intergenic |
| TCCCTATTAAATCCTTGAGT+TGG | 0.476466 | 7.4:-527175 | None:intergenic |
| AGCATTCTTGAACGATCAAA+TGG | 0.501702 | 7.4:-527232 | None:intergenic |
| GGATCAAGCCGTGACGATCA+AGG | 0.532320 | 7.4:+527136 | MS.gene050685:CDS |
| CGAATGCGGTGAACTACCAC+AGG | 0.532604 | 7.4:-527113 | None:intergenic |
| ACCACAGGGTGAACTACCAC+AGG | 0.535509 | 7.4:-527098 | None:intergenic |
| ATCGTTCAAGAATGCTCCAA+AGG | 0.558651 | 7.4:+527239 | MS.gene050685:CDS |
| CGTGTTGTAACTGCTGCAGT+TGG | 0.558826 | 7.4:+527275 | MS.gene050685:CDS |
| CAAGGATTTAATAGGGAAGG+TGG | 0.561353 | 7.4:+527181 | MS.gene050685:CDS |
| TCGTTCAAGAATGCTCCAAA+GGG | 0.578470 | 7.4:+527240 | MS.gene050685:CDS |
| CATTCTCACCTTGATCGTCA+CGG | 0.582038 | 7.4:-527144 | None:intergenic |
| GAAGATGAGCAACAAGGGAG+TGG | 0.588540 | 7.4:+527070 | MS.gene050685:CDS |
| GGAAAGAAGATGAGCAACAA+GGG | 0.598826 | 7.4:+527065 | MS.gene050685:CDS |
| TGGAAAGAAGATGAGCAACA+AGG | 0.599291 | 7.4:+527064 | MS.gene050685:CDS |
| AGAACATAGCGAAGATGTGT+AGG | 0.601986 | 7.4:+527352 | MS.gene050685:CDS |
| AATGACCAAAGGCCAACTCA+AGG | 0.609242 | 7.4:+527163 | MS.gene050685:CDS |
| TTCAAGAATGCTCCAAAGGG+AGG | 0.614086 | 7.4:+527243 | MS.gene050685:CDS |
| ACTCAAGGATTTAATAGGGA+AGG | 0.618810 | 7.4:+527178 | MS.gene050685:CDS |
| TCACGGCTTGATCCCGAATG+CGG | 0.664295 | 7.4:-527127 | None:intergenic |
| CAAGGGAGTGGTGCACCCTG+TGG | 0.671108 | 7.4:+527082 | MS.gene050685:CDS |
| ATCAAGGTGAGAATGACCAA+AGG | 0.688055 | 7.4:+527152 | MS.gene050685:CDS |
| CCCTGTGGTAGTTCACCCTG+TGG | 0.689874 | 7.4:+527097 | MS.gene050685:CDS |
| CCACAGGGTGAACTACCACA+GGG | 0.713193 | 7.4:-527097 | None:intergenic |
| GAATGCGGTGAACTACCACA+GGG | 0.781865 | 7.4:-527112 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAATGACAATTTAGAATT+AGG | + | chr7.4:527209-527228 | MS.gene050685:CDS | 20.0% |
| ! | ATACGTACAAGTTTTCAAAG+GGG | + | chr7.4:527309-527328 | MS.gene050685:CDS | 30.0% |
| ! | CATACGTACAAGTTTTCAAA+GGG | + | chr7.4:527308-527327 | MS.gene050685:CDS | 30.0% |
| ! | TCATACGTACAAGTTTTCAA+AGG | + | chr7.4:527307-527326 | MS.gene050685:CDS | 30.0% |
| ACTCAAGGATTTAATAGGGA+AGG | + | chr7.4:527178-527197 | MS.gene050685:CDS | 35.0% | |
| AGCATTCTTGAACGATCAAA+TGG | - | chr7.4:527235-527254 | None:intergenic | 35.0% | |
| GCCAACTCAAGGATTTAATA+GGG | + | chr7.4:527174-527193 | MS.gene050685:CDS | 35.0% | |
| TCCCTATTAAATCCTTGAGT+TGG | - | chr7.4:527178-527197 | None:intergenic | 35.0% | |
| TCTTCGCTATGTTCTTGAAT+TGG | - | chr7.4:527347-527366 | None:intergenic | 35.0% | |
| ATCAAGGTGAGAATGACCAA+AGG | + | chr7.4:527152-527171 | MS.gene050685:CDS | 40.0% | |
| ATCGTTCAAGAATGCTCCAA+AGG | + | chr7.4:527239-527258 | MS.gene050685:CDS | 40.0% | |
| CAAGGATTTAATAGGGAAGG+TGG | + | chr7.4:527181-527200 | MS.gene050685:CDS | 40.0% | |
| GGAAAGAAGATGAGCAACAA+GGG | + | chr7.4:527065-527084 | MS.gene050685:CDS | 40.0% | |
| GGCCAACTCAAGGATTTAAT+AGG | + | chr7.4:527173-527192 | MS.gene050685:CDS | 40.0% | |
| TCGTTCAAGAATGCTCCAAA+GGG | + | chr7.4:527240-527259 | MS.gene050685:CDS | 40.0% | |
| TGGAAAGAAGATGAGCAACA+AGG | + | chr7.4:527064-527083 | MS.gene050685:CDS | 40.0% | |
| TTTCAAAGGGGCTGAGTTTA+AGG | + | chr7.4:527321-527340 | MS.gene050685:CDS | 40.0% | |
| !! | TAAATCCTTGAGTTGGCCTT+TGG | - | chr7.4:527171-527190 | None:intergenic | 40.0% |
| AATGACCAAAGGCCAACTCA+AGG | + | chr7.4:527163-527182 | MS.gene050685:CDS | 45.0% | |
| CATTCTCACCTTGATCGTCA+CGG | - | chr7.4:527147-527166 | None:intergenic | 45.0% | |
| TTCAAGAATGCTCCAAAGGG+AGG | + | chr7.4:527243-527262 | MS.gene050685:CDS | 45.0% | |
| CGTGTTGTAACTGCTGCAGT+TGG | + | chr7.4:527275-527294 | MS.gene050685:CDS | 50.0% | |
| CTGTGGTAGTTCACCGCATT+CGG | + | chr7.4:527114-527133 | MS.gene050685:CDS | 50.0% | |
| GAAGATGAGCAACAAGGGAG+TGG | + | chr7.4:527070-527089 | MS.gene050685:CDS | 50.0% | |
| GAATGCGGTGAACTACCACA+GGG | - | chr7.4:527115-527134 | None:intergenic | 50.0% | |
| TGTGGTAGTTCACCGCATTC+GGG | + | chr7.4:527115-527134 | MS.gene050685:CDS | 50.0% | |
| ACCACAGGGTGAACTACCAC+AGG | - | chr7.4:527101-527120 | None:intergenic | 55.0% | |
| CCACAGGGTGAACTACCACA+GGG | - | chr7.4:527100-527119 | None:intergenic | 55.0% | |
| CGAATGCGGTGAACTACCAC+AGG | - | chr7.4:527116-527135 | None:intergenic | 55.0% | |
| GGATCAAGCCGTGACGATCA+AGG | + | chr7.4:527136-527155 | MS.gene050685:CDS | 55.0% | |
| TCACGGCTTGATCCCGAATG+CGG | - | chr7.4:527130-527149 | None:intergenic | 55.0% | |
| ACGAGCACGAAGCCTCCCTT+TGG | - | chr7.4:527258-527277 | None:intergenic | 60.0% | |
| CCCTGTGGTAGTTCACCCTG+TGG | + | chr7.4:527097-527116 | MS.gene050685:CDS | 60.0% | |
| CAAGGGAGTGGTGCACCCTG+TGG | + | chr7.4:527082-527101 | MS.gene050685:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 527047 | 527373 | 527047 | ID=MS.gene050685 |
| chr7.4 | mRNA | 527047 | 527373 | 527047 | ID=MS.gene050685.t1;Parent=MS.gene050685 |
| chr7.4 | exon | 527047 | 527373 | 527047 | ID=MS.gene050685.t1.exon1;Parent=MS.gene050685.t1 |
| chr7.4 | CDS | 527047 | 527373 | 527047 | ID=cds.MS.gene050685.t1;Parent=MS.gene050685.t1 |
| Gene Sequence |
| Protein sequence |
