Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22818.t1 | XP_003626542.2 | 75.9 | 141 | 29 | 3 | 1 | 141 | 30 | 165 | 9.60E-46 | 193 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22818.t1 | Q9SZC9 | 59.2 | 76 | 28 | 1 | 58 | 133 | 152 | 224 | 5.2e-16 | 85.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22818.t1 | G7L410 | 75.9 | 141 | 29 | 3 | 1 | 141 | 30 | 165 | 6.9e-46 | 193.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050625 | MS.gene22818 | 0.943437 | 1.21E-102 | -1.69E-46 |
| MS.gene050747 | MS.gene22818 | 0.81561 | 8.46E-52 | -1.69E-46 |
| MS.gene050950 | MS.gene22818 | 0.801935 | 7.12E-49 | -1.69E-46 |
| MS.gene052481 | MS.gene22818 | 0.818559 | 1.84E-52 | -1.69E-46 |
| MS.gene052982 | MS.gene22818 | 0.847732 | 9.60E-60 | -1.69E-46 |
| MS.gene054927 | MS.gene22818 | 0.835665 | 1.47E-56 | -1.69E-46 |
| MS.gene056166 | MS.gene22818 | 0.802321 | 5.93E-49 | -1.69E-46 |
| MS.gene058444 | MS.gene22818 | 0.816458 | 5.47E-52 | -1.69E-46 |
| MS.gene058700 | MS.gene22818 | 0.811461 | 6.92E-51 | -1.69E-46 |
| MS.gene06092 | MS.gene22818 | 0.800036 | 1.74E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22818.t1 | MTR_7g116990 | 87.234 | 141 | 13 | 3 | 1 | 141 | 30 | 165 | 5.31e-84 | 243 |
| MS.gene22818.t1 | MTR_4g094232 | 58.333 | 84 | 32 | 2 | 56 | 139 | 137 | 217 | 6.76e-25 | 99.4 |
| MS.gene22818.t1 | MTR_3g105190 | 41.667 | 84 | 42 | 1 | 53 | 136 | 84 | 160 | 1.75e-11 | 60.8 |
| MS.gene22818.t1 | MTR_3g105190 | 41.667 | 84 | 42 | 1 | 53 | 136 | 84 | 160 | 1.92e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22818.t1 | AT4G33520 | 51.042 | 96 | 44 | 1 | 46 | 141 | 140 | 232 | 3.98e-25 | 96.3 |
| MS.gene22818.t1 | AT4G33520 | 51.685 | 89 | 40 | 1 | 46 | 134 | 140 | 225 | 1.49e-24 | 94.7 |
| MS.gene22818.t1 | AT4G33520 | 50.549 | 91 | 42 | 1 | 43 | 133 | 137 | 224 | 5.22e-23 | 94.0 |
| MS.gene22818.t1 | AT4G33520 | 50.549 | 91 | 42 | 1 | 43 | 133 | 137 | 224 | 5.75e-23 | 94.0 |
Find 28 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCTCTGAATCTCAATTT+TGG | 0.195929 | 7.4:+1371714 | MS.gene22818:CDS |
| AAATATTACCTCTAAGTCTA+AGG | 0.276760 | 7.4:-1372338 | None:intergenic |
| ACTTCTACCCTTAGACTTAG+AGG | 0.293678 | 7.4:+1372330 | MS.gene22818:CDS |
| AAGAGAAAACTGCAACAAAT+TGG | 0.354991 | 7.4:+1372256 | MS.gene22818:CDS |
| GCTGTTGTTCTCCATGTTCG+TGG | 0.430325 | 7.4:+1371867 | MS.gene22818:CDS |
| TGAGCTTCAGCAGCTGCTGT+TGG | 0.445681 | 7.4:-1371752 | None:intergenic |
| ATCATGGAGGTGGTCCCCAC+TGG | 0.448930 | 7.4:-1371832 | None:intergenic |
| ACAAATTGGCAAAAGCAGTT+AGG | 0.468817 | 7.4:+1372270 | MS.gene22818:CDS |
| TTCGGATGACAAAGGGGAAA+GGG | 0.488766 | 7.4:-1372235 | None:intergenic |
| CTGCCAAAATTGAGATTCAG+AGG | 0.501848 | 7.4:-1371717 | None:intergenic |
| ACAGTAGCAGATAACACTTC+TGG | 0.505949 | 7.4:-1372183 | None:intergenic |
| TAAAACCACATGTTGTTAAG+TGG | 0.519304 | 7.4:-1372308 | None:intergenic |
| CACACATCATTCCACGAACA+TGG | 0.531138 | 7.4:-1371878 | None:intergenic |
| GTCCCCACTGGAAGTAGTTG+AGG | 0.533910 | 7.4:-1371820 | None:intergenic |
| CAGCAGAATCATCATCATCA+TGG | 0.554273 | 7.4:-1371848 | None:intergenic |
| CTTCGGATGACAAAGGGGAA+AGG | 0.559317 | 7.4:-1372236 | None:intergenic |
| GCACACCACTTAACAACATG+TGG | 0.559641 | 7.4:+1372303 | MS.gene22818:CDS |
| AATATTACCTCTAAGTCTAA+GGG | 0.564175 | 7.4:-1372337 | None:intergenic |
| AAACGCGATGATGATATTGA+TGG | 0.574757 | 7.4:-1371776 | None:intergenic |
| CAACCTCAACTACTTCCAGT+GGG | 0.602218 | 7.4:+1371817 | MS.gene22818:CDS |
| AATCATCATCATCATGGAGG+TGG | 0.613652 | 7.4:-1371842 | None:intergenic |
| TCAACCTCAACTACTTCCAG+TGG | 0.617567 | 7.4:+1371816 | MS.gene22818:CDS |
| GAGGTTGAACAACACCGCAA+TGG | 0.646311 | 7.4:-1371801 | None:intergenic |
| TTTCTCTTCGGATGACAAAG+GGG | 0.661704 | 7.4:-1372241 | None:intergenic |
| GTTCGTGGAATGATGTGTGA+GGG | 0.680994 | 7.4:+1371882 | MS.gene22818:CDS |
| TGTTCGTGGAATGATGTGTG+AGG | 0.700135 | 7.4:+1371881 | MS.gene22818:CDS |
| CAGAATCATCATCATCATGG+AGG | 0.719272 | 7.4:-1371845 | None:intergenic |
| AACCTCAACTACTTCCAGTG+GGG | 0.733472 | 7.4:+1371818 | MS.gene22818:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACATTAATTATAATCG+AGG | + | chr7.4:1372030-1372049 | MS.gene22818:intron | 15.0% |
| !!! | TCAAAAAGTTTGATTTTAAA+AGG | + | chr7.4:1372054-1372073 | MS.gene22818:intron | 15.0% |
| !!! | TTTTTTCAAATTTCATGTAA+AGG | + | chr7.4:1372397-1372416 | MS.gene22818:intron | 15.0% |
| !!! | ATTGCAAATGTTGATAAAAA+AGG | - | chr7.4:1372364-1372383 | None:intergenic | 20.0% |
| ! | AAATATTACCTCTAAGTCTA+AGG | - | chr7.4:1372341-1372360 | None:intergenic | 25.0% |
| ! | AATATTACCTCTAAGTCTAA+GGG | - | chr7.4:1372340-1372359 | None:intergenic | 25.0% |
| ! | CAAATTTCATGTAAAGGAAA+AGG | + | chr7.4:1372403-1372422 | MS.gene22818:intron | 25.0% |
| ! | CTCTATATAATCTCATTTAG+AGG | - | chr7.4:1372764-1372783 | None:intergenic | 25.0% |
| ! | GTTTAATGAGTAAATGACAA+TGG | - | chr7.4:1372591-1372610 | None:intergenic | 25.0% |
| ! | TATATTGCAGTGATAGTATA+CGG | - | chr7.4:1372130-1372149 | None:intergenic | 25.0% |
| ! | TCAAATTTCATTTACAGACA+AGG | - | chr7.4:1372656-1372675 | None:intergenic | 25.0% |
| ! | TGACATGCTAAATTGAATAA+AGG | - | chr7.4:1372707-1372726 | None:intergenic | 25.0% |
| !! | AATTTGATTTACATGAAGCA+TGG | + | chr7.4:1372786-1372805 | MS.gene22818:intron | 25.0% |
| AAGAGAAAACTGCAACAAAT+TGG | + | chr7.4:1372256-1372275 | MS.gene22818:CDS | 30.0% | |
| AATTGCGTAGATATGAACAA+AGG | - | chr7.4:1372616-1372635 | None:intergenic | 30.0% | |
| CGCGAGTTTGATAAAATAAA+GGG | + | chr7.4:1372088-1372107 | MS.gene22818:intron | 30.0% | |
| GTTCATATCTACGCAATTAA+TGG | + | chr7.4:1372618-1372637 | MS.gene22818:intron | 30.0% | |
| TAAAACCACATGTTGTTAAG+TGG | - | chr7.4:1372311-1372330 | None:intergenic | 30.0% | |
| TACGTCTAACAAAATCAAGA+CGG | + | chr7.4:1372520-1372539 | MS.gene22818:intron | 30.0% | |
| TCATTTACAGACAAGGAAAA+TGG | - | chr7.4:1372649-1372668 | None:intergenic | 30.0% | |
| TCGCGAGTTTGATAAAATAA+AGG | + | chr7.4:1372087-1372106 | MS.gene22818:intron | 30.0% | |
| TGTGAATGCAAACATTATTC+AGG | - | chr7.4:1372563-1372582 | None:intergenic | 30.0% | |
| ! | AAACCTCTGAATCTCAATTT+TGG | + | chr7.4:1371714-1371733 | MS.gene22818:CDS | 30.0% |
| ! | TGATTTTGTTAGACGTAATG+CGG | - | chr7.4:1372517-1372536 | None:intergenic | 30.0% |
| !! | ATTTGTTGCAGTTTTCTCTT+CGG | - | chr7.4:1372256-1372275 | None:intergenic | 30.0% |
| AAAAGCCCCTAAAACATAGA+GGG | - | chr7.4:1372683-1372702 | None:intergenic | 35.0% | |
| AAACGCGATGATGATATTGA+TGG | - | chr7.4:1371779-1371798 | None:intergenic | 35.0% | |
| ACAAATTGGCAAAAGCAGTT+AGG | + | chr7.4:1372270-1372289 | MS.gene22818:CDS | 35.0% | |
| ACGTCTAACAAAATCAAGAC+GGG | + | chr7.4:1372521-1372540 | MS.gene22818:intron | 35.0% | |
| TAAAAGCCCCTAAAACATAG+AGG | - | chr7.4:1372684-1372703 | None:intergenic | 35.0% | |
| TTGATTTACATGAAGCATGG+TGG | + | chr7.4:1372789-1372808 | MS.gene22818:intron | 35.0% | |
| ! | GAAAACCCCTCTATGTTTTA+GGG | + | chr7.4:1372674-1372693 | MS.gene22818:intron | 35.0% |
| ! | TGAAAACCCCTCTATGTTTT+AGG | + | chr7.4:1372673-1372692 | MS.gene22818:intron | 35.0% |
| ! | TTTTCTCTTCGGATGACAAA+GGG | - | chr7.4:1372245-1372264 | None:intergenic | 35.0% |
| !! | AAAACCCCTCTATGTTTTAG+GGG | + | chr7.4:1372675-1372694 | MS.gene22818:intron | 35.0% |
| AAAGCCCCTAAAACATAGAG+GGG | - | chr7.4:1372682-1372701 | None:intergenic | 40.0% | |
| AATCATCATCATCATGGAGG+TGG | - | chr7.4:1371845-1371864 | None:intergenic | 40.0% | |
| ACAGTAGCAGATAACACTTC+TGG | - | chr7.4:1372186-1372205 | None:intergenic | 40.0% | |
| ACTTCTACCCTTAGACTTAG+AGG | + | chr7.4:1372330-1372349 | MS.gene22818:CDS | 40.0% | |
| CAGAATCATCATCATCATGG+AGG | - | chr7.4:1371848-1371867 | None:intergenic | 40.0% | |
| CAGCAGAATCATCATCATCA+TGG | - | chr7.4:1371851-1371870 | None:intergenic | 40.0% | |
| CATGTCACTAAGTATGCTTG+TGG | + | chr7.4:1372720-1372739 | MS.gene22818:intron | 40.0% | |
| CTGCCAAAATTGAGATTCAG+AGG | - | chr7.4:1371720-1371739 | None:intergenic | 40.0% | |
| TTTCTCTTCGGATGACAAAG+GGG | - | chr7.4:1372244-1372263 | None:intergenic | 40.0% | |
| ! | GTTTTCTCTTCGGATGACAA+AGG | - | chr7.4:1372246-1372265 | None:intergenic | 40.0% |
| AACCTCAACTACTTCCAGTG+GGG | + | chr7.4:1371818-1371837 | MS.gene22818:CDS | 45.0% | |
| CAACCTCAACTACTTCCAGT+GGG | + | chr7.4:1371817-1371836 | MS.gene22818:CDS | 45.0% | |
| CACACATCATTCCACGAACA+TGG | - | chr7.4:1371881-1371900 | None:intergenic | 45.0% | |
| GCACACCACTTAACAACATG+TGG | + | chr7.4:1372303-1372322 | MS.gene22818:CDS | 45.0% | |
| GTTCGTGGAATGATGTGTGA+GGG | + | chr7.4:1371882-1371901 | MS.gene22818:CDS | 45.0% | |
| TCAACCTCAACTACTTCCAG+TGG | + | chr7.4:1371816-1371835 | MS.gene22818:CDS | 45.0% | |
| TGTTCGTGGAATGATGTGTG+AGG | + | chr7.4:1371881-1371900 | MS.gene22818:CDS | 45.0% | |
| TTCGGATGACAAAGGGGAAA+GGG | - | chr7.4:1372238-1372257 | None:intergenic | 45.0% | |
| ! | ATCATCGCGTTTTGCCATTG+CGG | + | chr7.4:1371787-1371806 | MS.gene22818:CDS | 45.0% |
| !! | AAGCATGGTGGATTAAGTGC+AGG | + | chr7.4:1372801-1372820 | MS.gene22818:intron | 45.0% |
| !! | CTCAATTTTGGCAGAGACAG+AGG | + | chr7.4:1371726-1371745 | MS.gene22818:CDS | 45.0% |
| !! | TGTTATTAATAAATAATTAA+TGG | + | chr7.4:1372156-1372175 | MS.gene22818:intron | 5.0% |
| CTTCGGATGACAAAGGGGAA+AGG | - | chr7.4:1372239-1372258 | None:intergenic | 50.0% | |
| GAGGTTGAACAACACCGCAA+TGG | - | chr7.4:1371804-1371823 | None:intergenic | 50.0% | |
| GCTGTTGTTCTCCATGTTCG+TGG | + | chr7.4:1371867-1371886 | MS.gene22818:CDS | 50.0% | |
| ! | AAATCAAGACGGGCTCTCTG+CGG | + | chr7.4:1372531-1372550 | MS.gene22818:intron | 50.0% |
| GTCCCCACTGGAAGTAGTTG+AGG | - | chr7.4:1371823-1371842 | None:intergenic | 55.0% | |
| TGAGCTTCAGCAGCTGCTGT+TGG | - | chr7.4:1371755-1371774 | None:intergenic | 55.0% | |
| ! | AATCAAGACGGGCTCTCTGC+GGG | + | chr7.4:1372532-1372551 | MS.gene22818:intron | 55.0% |
| ATCATGGAGGTGGTCCCCAC+TGG | - | chr7.4:1371835-1371854 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 1371705 | 1372842 | 1371705 | ID=MS.gene22818 |
| chr7.4 | mRNA | 1371705 | 1372842 | 1371705 | ID=MS.gene22818.t1;Parent=MS.gene22818 |
| chr7.4 | exon | 1371705 | 1371941 | 1371705 | ID=MS.gene22818.t1.exon1;Parent=MS.gene22818.t1 |
| chr7.4 | CDS | 1371705 | 1371941 | 1371705 | ID=cds.MS.gene22818.t1;Parent=MS.gene22818.t1 |
| chr7.4 | exon | 1372183 | 1372351 | 1372183 | ID=MS.gene22818.t1.exon2;Parent=MS.gene22818.t1 |
| chr7.4 | CDS | 1372183 | 1372351 | 1372183 | ID=cds.MS.gene22818.t1;Parent=MS.gene22818.t1 |
| chr7.4 | exon | 1372823 | 1372842 | 1372823 | ID=MS.gene22818.t1.exon3;Parent=MS.gene22818.t1 |
| chr7.4 | CDS | 1372823 | 1372842 | 1372823 | ID=cds.MS.gene22818.t1;Parent=MS.gene22818.t1 |
| Gene Sequence |
| Protein sequence |
