AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050625


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050625.t1 MTR_7g116990 83.750 160 17 4 12 167 11 165 5.64e-90 259
MS.gene050625.t1 MTR_4g094232 61.538 78 27 2 82 159 137 211 2.41e-24 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050625.t1 AT4G33520 51.042 96 44 1 72 167 140 232 1.08e-24 95.9
MS.gene050625.t1 AT4G33520 52.174 92 40 2 72 162 140 228 3.87e-24 94.4
MS.gene050625.t1 AT4G33520 57.692 78 30 1 82 159 150 224 1.77e-22 93.6
MS.gene050625.t1 AT4G33520 57.692 78 30 1 82 159 150 224 1.77e-22 93.6

Find 30 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCTCTGAATCTCAATTT+TGG 0.195929 7.3:+1185037 MS.gene050625:CDS
AAATATTACCTCTAAGTCTA+AGG 0.276760 7.3:-1185652 None:intergenic
ACTTCTACCCTTAGACTTAG+AGG 0.293678 7.3:+1185644 MS.gene050625:CDS
TGCGTCACAGCAGAATGATT+TGG 0.295260 7.3:-1185001 None:intergenic
GCGTCACAGCAGAATGATTT+GGG 0.352377 7.3:-1185000 None:intergenic
AAGAGAAAACTGCAACAAAT+TGG 0.354991 7.3:+1185570 MS.gene050625:CDS
GCTGTTGTTCTCCATGTTCG+TGG 0.430325 7.3:+1185190 MS.gene050625:CDS
TGAGCTTCAGCAGCTGCTGT+TGG 0.445681 7.3:-1185075 None:intergenic
ATCATGGAGGTGGTCCCCAC+TGG 0.448930 7.3:-1185155 None:intergenic
ACAAATTGGCAAAAGCAGTT+AGG 0.468817 7.3:+1185584 MS.gene050625:CDS
TTCGGATGACAAAGGGGAAA+GGG 0.488766 7.3:-1185549 None:intergenic
CTGCCAAAATTGAGATTCAG+AGG 0.501848 7.3:-1185040 None:intergenic
ACAGTAGCAGATAACACTTC+TGG 0.505949 7.3:-1185497 None:intergenic
TAAAACCACATGTTGTTAAG+TGG 0.519304 7.3:-1185622 None:intergenic
CACACATCATTCCACGAACA+TGG 0.531138 7.3:-1185201 None:intergenic
GTCCCCACTGGAAGTAGTTG+AGG 0.533910 7.3:-1185143 None:intergenic
CAGCAGAATCATCATCATCA+TGG 0.554273 7.3:-1185171 None:intergenic
CTTCGGATGACAAAGGGGAA+AGG 0.559317 7.3:-1185550 None:intergenic
GCACACCACTTAACAACATG+TGG 0.559641 7.3:+1185617 MS.gene050625:CDS
AATATTACCTCTAAGTCTAA+GGG 0.564175 7.3:-1185651 None:intergenic
AAACGCGATGATGATATTGA+TGG 0.574757 7.3:-1185099 None:intergenic
CAACCTCAACTACTTCCAGT+GGG 0.602218 7.3:+1185140 MS.gene050625:CDS
AATCATCATCATCATGGAGG+TGG 0.613652 7.3:-1185165 None:intergenic
TCAACCTCAACTACTTCCAG+TGG 0.617567 7.3:+1185139 MS.gene050625:CDS
GAGGTTGAACAACACCGCAA+TGG 0.646311 7.3:-1185124 None:intergenic
TTTCTCTTCGGATGACAAAG+GGG 0.661704 7.3:-1185555 None:intergenic
GTTCGTGGAATGATGTGTGA+GGG 0.680994 7.3:+1185205 MS.gene050625:CDS
TGTTCGTGGAATGATGTGTG+AGG 0.700135 7.3:+1185204 MS.gene050625:CDS
CAGAATCATCATCATCATGG+AGG 0.719272 7.3:-1185168 None:intergenic
AACCTCAACTACTTCCAGTG+GGG 0.733472 7.3:+1185141 MS.gene050625:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAACATTGATTTTAAA+AGG + chr7.3:1185353-1185372 MS.gene050625:intron 10.0%
!!! TTTTTTCAAATTTCATGTAA+AGG + chr7.3:1185711-1185730 MS.gene050625:intron 15.0%
!!! ATTGCAAATGTTGATAAAAA+AGG - chr7.3:1185678-1185697 None:intergenic 20.0%
! AAATATTACCTCTAAGTCTA+AGG - chr7.3:1185655-1185674 None:intergenic 25.0%
! AATATTACCTCTAAGTCTAA+GGG - chr7.3:1185654-1185673 None:intergenic 25.0%
! CAAATTTCATGTAAAGGAAA+AGG + chr7.3:1185717-1185736 MS.gene050625:intron 25.0%
! CTCTATATAATCTCATTTAG+AGG - chr7.3:1186078-1186097 None:intergenic 25.0%
! TATATTGCAGTGATAGTATA+CGG - chr7.3:1185444-1185463 None:intergenic 25.0%
! TCAAATTTCATTTACAGACA+AGG - chr7.3:1185970-1185989 None:intergenic 25.0%
! TGACATGCTAAATTGAATAA+AGG - chr7.3:1186021-1186040 None:intergenic 25.0%
!! AATTTGATTTACATGAAGCA+TGG + chr7.3:1186100-1186119 MS.gene050625:intron 25.0%
AAGAGAAAACTGCAACAAAT+TGG + chr7.3:1185570-1185589 MS.gene050625:CDS 30.0%
AATTGCGTAGATATGAACAA+AGG - chr7.3:1185930-1185949 None:intergenic 30.0%
CGCGAGTTTGATAAAATAAA+GGG + chr7.3:1185387-1185406 MS.gene050625:intron 30.0%
GTTCATATCTACGCAATTAA+TGG + chr7.3:1185932-1185951 MS.gene050625:intron 30.0%
GTTTCATGAGTAAATGACAA+TGG - chr7.3:1185905-1185924 None:intergenic 30.0%
TAAAACCACATGTTGTTAAG+TGG - chr7.3:1185625-1185644 None:intergenic 30.0%
TACGTCTAACAAAATCAAGA+CGG + chr7.3:1185834-1185853 MS.gene050625:intron 30.0%
TCATTTACAGACAAGGAAAA+TGG - chr7.3:1185963-1185982 None:intergenic 30.0%
TCGCGAGTTTGATAAAATAA+AGG + chr7.3:1185386-1185405 MS.gene050625:intron 30.0%
TGTGAATGCAAACATTATTC+AGG - chr7.3:1185877-1185896 None:intergenic 30.0%
! AAACCTCTGAATCTCAATTT+TGG + chr7.3:1185037-1185056 MS.gene050625:CDS 30.0%
! TGATTTTGTTAGACGTAATG+CGG - chr7.3:1185831-1185850 None:intergenic 30.0%
!! ATTTGTTGCAGTTTTCTCTT+CGG - chr7.3:1185570-1185589 None:intergenic 30.0%
AAAAGCCCCTAAAACATAGA+GGG - chr7.3:1185997-1186016 None:intergenic 35.0%
AAACGCGATGATGATATTGA+TGG - chr7.3:1185102-1185121 None:intergenic 35.0%
ACAAATTGGCAAAAGCAGTT+AGG + chr7.3:1185584-1185603 MS.gene050625:CDS 35.0%
ACGTCTAACAAAATCAAGAC+GGG + chr7.3:1185835-1185854 MS.gene050625:intron 35.0%
TAAAAGCCCCTAAAACATAG+AGG - chr7.3:1185998-1186017 None:intergenic 35.0%
TTGATTTACATGAAGCATGG+TGG + chr7.3:1186103-1186122 MS.gene050625:intron 35.0%
! GAAAACCCCTCTATGTTTTA+GGG + chr7.3:1185988-1186007 MS.gene050625:intron 35.0%
! TGAAAACCCCTCTATGTTTT+AGG + chr7.3:1185987-1186006 MS.gene050625:intron 35.0%
! TTTTCTCTTCGGATGACAAA+GGG - chr7.3:1185559-1185578 None:intergenic 35.0%
!! AAAACCCCTCTATGTTTTAG+GGG + chr7.3:1185989-1186008 MS.gene050625:intron 35.0%
AAAGCCCCTAAAACATAGAG+GGG - chr7.3:1185996-1186015 None:intergenic 40.0%
AATCATCATCATCATGGAGG+TGG - chr7.3:1185168-1185187 None:intergenic 40.0%
ACAGTAGCAGATAACACTTC+TGG - chr7.3:1185500-1185519 None:intergenic 40.0%
ACTTCTACCCTTAGACTTAG+AGG + chr7.3:1185644-1185663 MS.gene050625:CDS 40.0%
CAGAATCATCATCATCATGG+AGG - chr7.3:1185171-1185190 None:intergenic 40.0%
CAGCAGAATCATCATCATCA+TGG - chr7.3:1185174-1185193 None:intergenic 40.0%
CATGTCACTAAGTATGCTTG+TGG + chr7.3:1186034-1186053 MS.gene050625:intron 40.0%
CTGCCAAAATTGAGATTCAG+AGG - chr7.3:1185043-1185062 None:intergenic 40.0%
TTTCTCTTCGGATGACAAAG+GGG - chr7.3:1185558-1185577 None:intergenic 40.0%
! GTTTTCTCTTCGGATGACAA+AGG - chr7.3:1185560-1185579 None:intergenic 40.0%
AACCTCAACTACTTCCAGTG+GGG + chr7.3:1185141-1185160 MS.gene050625:CDS 45.0%
CAACCTCAACTACTTCCAGT+GGG + chr7.3:1185140-1185159 MS.gene050625:CDS 45.0%
CACACATCATTCCACGAACA+TGG - chr7.3:1185204-1185223 None:intergenic 45.0%
GCACACCACTTAACAACATG+TGG + chr7.3:1185617-1185636 MS.gene050625:CDS 45.0%
GCGTCACAGCAGAATGATTT+GGG - chr7.3:1185003-1185022 None:intergenic 45.0%
GTTCGTGGAATGATGTGTGA+GGG + chr7.3:1185205-1185224 MS.gene050625:CDS 45.0%
TCAACCTCAACTACTTCCAG+TGG + chr7.3:1185139-1185158 MS.gene050625:CDS 45.0%
TGCGTCACAGCAGAATGATT+TGG - chr7.3:1185004-1185023 None:intergenic 45.0%
TGTTCGTGGAATGATGTGTG+AGG + chr7.3:1185204-1185223 MS.gene050625:CDS 45.0%
TTCGGATGACAAAGGGGAAA+GGG - chr7.3:1185552-1185571 None:intergenic 45.0%
! ATCATCGCGTTTTGCCATTG+CGG + chr7.3:1185110-1185129 MS.gene050625:CDS 45.0%
!! AAGCATGGTGGATTAAGTGC+AGG + chr7.3:1186115-1186134 MS.gene050625:intron 45.0%
!! CTCAATTTTGGCAGAGACAG+AGG + chr7.3:1185049-1185068 MS.gene050625:CDS 45.0%
!! TGTTATTAATAAATAATTAA+TGG + chr7.3:1185470-1185489 MS.gene050625:intron 5.0%
CTTCGGATGACAAAGGGGAA+AGG - chr7.3:1185553-1185572 None:intergenic 50.0%
GAGGTTGAACAACACCGCAA+TGG - chr7.3:1185127-1185146 None:intergenic 50.0%
GCTGTTGTTCTCCATGTTCG+TGG + chr7.3:1185190-1185209 MS.gene050625:CDS 50.0%
! AAATCAAGACGGGCTCTCTG+CGG + chr7.3:1185845-1185864 MS.gene050625:intron 50.0%
GTCCCCACTGGAAGTAGTTG+AGG - chr7.3:1185146-1185165 None:intergenic 55.0%
TGAGCTTCAGCAGCTGCTGT+TGG - chr7.3:1185078-1185097 None:intergenic 55.0%
! AATCAAGACGGGCTCTCTGC+GGG + chr7.3:1185846-1185865 MS.gene050625:intron 55.0%
ATCATGGAGGTGGTCCCCAC+TGG - chr7.3:1185158-1185177 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 1184950 1186156 1184950 ID=MS.gene050625
chr7.3 mRNA 1184950 1186156 1184950 ID=MS.gene050625.t1;Parent=MS.gene050625
chr7.3 exon 1184950 1185264 1184950 ID=MS.gene050625.t1.exon1;Parent=MS.gene050625.t1
chr7.3 CDS 1184950 1185264 1184950 ID=cds.MS.gene050625.t1;Parent=MS.gene050625.t1
chr7.3 exon 1185497 1185665 1185497 ID=MS.gene050625.t1.exon2;Parent=MS.gene050625.t1
chr7.3 CDS 1185497 1185665 1185497 ID=cds.MS.gene050625.t1;Parent=MS.gene050625.t1
chr7.3 exon 1186137 1186156 1186137 ID=MS.gene050625.t1.exon3;Parent=MS.gene050625.t1
chr7.3 CDS 1186137 1186156 1186137 ID=cds.MS.gene050625.t1;Parent=MS.gene050625.t1
Gene Sequence

>MS.gene050625

ATGTCATCATCATCATCTTGTTCTTGTTCTTCGATTCGTGTACTTCAAATCCCAAATCATTCTGCTGTGACGCAAAAGATGTTTATGAAACCTCTGAATCTCAATTTTGGCAGAGACAGAGGCATCCAACAGCAGCTGCTGAAGCTCAACCATCAATATCATCATCGCGTTTTGCCATTGCGGTGTTGTTCAACCTCAACTACTTCCAGTGGGGACCACCTCCATGATGATGATGATTCTGCTGTTGTTCTCCATGTTCGTGGAATGATGTGTGAGGGATGTGCTTCTAGCGTCAAAAAACTTCTTGAAACTCAAGTAAGTATGTCGTCTATTTCTATTTCTATTTTCGAGTTAATTCGCATTTTCTATTTGAATTCATTAATTTTAATTATTTCTCAAGAAAAAAAAAACATTGATTTTAAAAGGAAAACGATTTTCGCGAGTTTGATAAAATAAAGGGACTAAACATGCAATAAGCATTTTTTTTTTTTCCGTATACTATCACTGCAATATAAATGTTTGTTATTAATAAATAATTAATGGACAGCCAGAAGTGTTATCTGCTACTGTGAATCTAGCATCAGAGACAGCACTAGTTTCCCTTTCCCCTTTGTCATCCGAAGAGAAAACTGCAACAAATTGGCAAAAGCAGTTAGGAGAGAAACTTGCACACCACTTAACAACATGTGGTTTTACTTCTACCCTTAGACTTAGAGGTAATATTTCCTTTTTTATCAACATTTGCAATATTAGCTTGTTTGTTTTTTCAAATTTCATGTAAAGGAAAAGGTGTTGCTTGAGTTTATAGAGATGATTGTGTAGCATGTCATCATACTTTTTAATGCATAAAGCTGTATTAACTAACATTCTTCATATAGCCGCATTACGTCTAACAAAATCAAGACGGGCTCTCTGCGGGAGTTGCCTGAATAATGTTTGCATTCACACTTCTCCATTGTCATTTACTCATGAAACTTCCTTTGTTCATATCTACGCAATTAATGGTATATCCATTTTCCTTGTCTGTAAATGAAATTTGAAAACCCCTCTATGTTTTAGGGGCTTTTACCTTTATTCAATTTAGCATGTCACTAAGTATGCTTGTGGTTTACTTATTAATTATTGCCTCTAAATGAGATTATATAGAGATAATTTGATTTACATGAAGCATGGTGGATTAAGTGCAGGTCAAGAAGATAGCGCCTAA

Protein sequence

>MS.gene050625.t1

MSSSSSCSCSSIRVLQIPNHSAVTQKMFMKPLNLNFGRDRGIQQQLLKLNHQYHHRVLPLRCCSTSTTSSGDHLHDDDDSAVVLHVRGMMCEGCASSVKKLLETQPEVLSATVNLASETALVSLSPLSSEEKTATNWQKQLGEKLAHHLTTCGFTSTLRLRGQEDSA