Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22847.t1 | RHN49490.1 | 84.4 | 173 | 18 | 3 | 2 | 172 | 3 | 168 | 9.50E-72 | 279.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22847.t1 | A0A396H898 | 84.4 | 173 | 18 | 3 | 2 | 172 | 3 | 168 | 6.9e-72 | 279.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050592 | MS.gene22847 | 0.986238 | 4.00E-166 | -1.69E-46 |
| MS.gene05985 | MS.gene22847 | -0.809707 | 1.66E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22847.t1 | MTR_7g116610 | 91.716 | 169 | 5 | 3 | 6 | 172 | 3 | 164 | 7.61e-109 | 307 |
| MS.gene22847.t1 | MTR_8g011470 | 33.742 | 163 | 79 | 5 | 9 | 169 | 11 | 146 | 2.04e-18 | 77.4 |
| MS.gene22847.t1 | MTR_8g011470 | 36.842 | 152 | 60 | 6 | 9 | 155 | 11 | 131 | 4.84e-16 | 70.9 |
| MS.gene22847.t1 | MTR_7g052620 | 29.775 | 178 | 84 | 9 | 4 | 172 | 6 | 151 | 1.33e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22847.t1 | AT5G18310 | 34.911 | 169 | 94 | 5 | 5 | 169 | 11 | 167 | 1.62e-20 | 83.6 |
| MS.gene22847.t1 | AT5G48500 | 30.636 | 173 | 94 | 6 | 3 | 167 | 9 | 163 | 5.26e-14 | 66.6 |
| MS.gene22847.t1 | AT5G18310 | 31.579 | 152 | 88 | 5 | 22 | 169 | 1 | 140 | 1.18e-11 | 59.7 |
Find 35 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTCAAAGTATTGAGTTAT+TGG | 0.194110 | 7.4:-1612741 | None:intergenic |
| TCTCATTCACCATAACATTT+TGG | 0.210593 | 7.4:-1613343 | None:intergenic |
| TTCTTGTTCATTTGCTTCCT+TGG | 0.212136 | 7.4:-1613437 | None:intergenic |
| AGATCTTCATCAACAGGTTT+TGG | 0.271957 | 7.4:-1613497 | None:intergenic |
| AAATACCAGCTTTCGGAAAC+TGG | 0.279440 | 7.4:+1612700 | MS.gene22847:CDS |
| ATGTGTAGGCAACAAGGAAT+AGG | 0.352813 | 7.4:+1613309 | MS.gene22847:intron |
| TTATAGAGATCTTCATCAAC+AGG | 0.392468 | 7.4:-1613503 | None:intergenic |
| GGGTTTCATTTCCAAGTGTC+TGG | 0.396397 | 7.4:+1613678 | MS.gene22847:CDS |
| GGAAGTCTCGTTGGTTGTAA+TGG | 0.406598 | 7.4:-1613476 | None:intergenic |
| ATTCGTTACAGTTCTTCATC+TGG | 0.411701 | 7.4:+1612783 | MS.gene22847:CDS |
| AGTTGTCAAATACCAGCTTT+CGG | 0.450434 | 7.4:+1612693 | MS.gene22847:CDS |
| GTGGTTGTTACCTTTCGAAC+TGG | 0.453480 | 7.4:-1612882 | None:intergenic |
| AAGGAAGTACCAAAATGTTA+TGG | 0.457410 | 7.4:+1613334 | MS.gene22847:CDS |
| AGCTACTTCGCACAACTAAG+AGG | 0.477902 | 7.4:+1613537 | MS.gene22847:CDS |
| AAATCCCAGTTTCCGAAAGC+TGG | 0.479485 | 7.4:-1612705 | None:intergenic |
| TTAGTTGTGCGAAGTAGCTC+AGG | 0.482904 | 7.4:-1613533 | None:intergenic |
| TTTGAAACTGCAAGACAAGC+TGG | 0.494930 | 7.4:+1612756 | MS.gene22847:CDS |
| AGCAGCAGGCACCAGACACT+TGG | 0.496535 | 7.4:-1613689 | None:intergenic |
| AGGGAAAGTGTACGACGTAA+TGG | 0.509693 | 7.4:+1613409 | MS.gene22847:CDS |
| TGTGTAGGCAACAAGGAATA+GGG | 0.512863 | 7.4:+1613310 | MS.gene22847:intron |
| CTGCTGCTTGTGTTTCATGA+GGG | 0.515777 | 7.4:+1613704 | MS.gene22847:CDS |
| CCTGCTGCTTGTGTTTCATG+AGG | 0.531196 | 7.4:+1613703 | MS.gene22847:CDS |
| AATACCAGCTTTCGGAAACT+GGG | 0.543714 | 7.4:+1612701 | MS.gene22847:CDS |
| CCTCATGAAACACAAGCAGC+AGG | 0.582533 | 7.4:-1613703 | None:intergenic |
| TAATAAGAAACCAGTTCGAA+AGG | 0.596559 | 7.4:+1612872 | MS.gene22847:CDS |
| GCTGCACGAACATGAACGTA+AGG | 0.605636 | 7.4:-1612816 | None:intergenic |
| TTTGTTATGTGTAGGCAACA+AGG | 0.611013 | 7.4:+1613303 | MS.gene22847:intron |
| AAGGAAGCAAATGAACAAGA+AGG | 0.611603 | 7.4:+1613439 | MS.gene22847:CDS |
| TGAACATGAGAAAGCAGCAA+GGG | 0.621624 | 7.4:+1613390 | MS.gene22847:CDS |
| GTTGTGCGAAGTAGCTCAGG+AGG | 0.623117 | 7.4:-1613530 | None:intergenic |
| GCTACTTCGCACAACTAAGA+GGG | 0.636297 | 7.4:+1613538 | MS.gene22847:CDS |
| AGGCAACAAGGAATAGGGAA+AGG | 0.648026 | 7.4:+1613315 | MS.gene22847:intron |
| ATGAACATGAGAAAGCAGCA+AGG | 0.652015 | 7.4:+1613389 | MS.gene22847:CDS |
| ACGACGTAATGGAACATCCA+AGG | 0.662179 | 7.4:+1613420 | MS.gene22847:CDS |
| TTGTGCGAAGTAGCTCAGGA+GGG | 0.688459 | 7.4:-1613529 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTTTTTATTTACTAGT+GGG | + | chr7.4:1613219-1613238 | MS.gene22847:intron | 10.0% |
| !! | CACTAGTAAATAAAAAAAAT+AGG | - | chr7.4:1613220-1613239 | None:intergenic | 15.0% |
| !!! | AGTAGTACAAAAATAATTTT+TGG | + | chr7.4:1613247-1613266 | MS.gene22847:intron | 15.0% |
| !!! | CTATTTTTTTTATTTACTAG+TGG | + | chr7.4:1613218-1613237 | MS.gene22847:intron | 15.0% |
| !! | AAAGTTGTAAGGTTAAAATT+TGG | - | chr7.4:1612946-1612965 | None:intergenic | 20.0% |
| !! | AAGAACAAGAAAAAACAAAA+AGG | - | chr7.4:1612907-1612926 | None:intergenic | 20.0% |
| !! | AATCACATTATTAACCATAT+GGG | - | chr7.4:1613156-1613175 | None:intergenic | 20.0% |
| !! | ATTAACAACATTTCAAATCA+CGG | - | chr7.4:1613619-1613638 | None:intergenic | 20.0% |
| !!! | GTACAAAAATAATTTTTGGT+TGG | + | chr7.4:1613251-1613270 | MS.gene22847:intron | 20.0% |
| !!! | TAATGTTTGTTGTTGTTAAT+AGG | + | chr7.4:1612659-1612678 | MS.gene22847:intron | 20.0% |
| !!! | TTATATGATTTGTTATGTGT+AGG | + | chr7.4:1613295-1613314 | MS.gene22847:intron | 20.0% |
| ! | AACAAGAAAAAACAAAAAGG+TGG | - | chr7.4:1612904-1612923 | None:intergenic | 25.0% |
| ! | ATGGTTAATAATGTGATTGA+TGG | + | chr7.4:1613158-1613177 | MS.gene22847:intron | 25.0% |
| ! | CAATCACATTATTAACCATA+TGG | - | chr7.4:1613157-1613176 | None:intergenic | 25.0% |
| ! | CAATGATTATGAAAGTTGTA+AGG | - | chr7.4:1612957-1612976 | None:intergenic | 25.0% |
| ! | TTTGTGCAGAAAAAAATGTT+GGG | + | chr7.4:1613658-1613677 | MS.gene22847:intron | 25.0% |
| !! | TGAGTTAGATTTTTCATTTG+AGG | - | chr7.4:1612989-1613008 | None:intergenic | 25.0% |
| !!! | ACTATTTACTCTGAAATTTG+TGG | + | chr7.4:1613091-1613110 | MS.gene22847:intron | 25.0% |
| !!! | GTTTCAAAGTATTGAGTTAT+TGG | - | chr7.4:1612744-1612763 | None:intergenic | 25.0% |
| !!! | TTTGTTGTTGTTAATAGGAA+TGG | + | chr7.4:1612664-1612683 | MS.gene22847:intron | 25.0% |
| GTTTGTGCAGAAAAAAATGT+TGG | + | chr7.4:1613657-1613676 | MS.gene22847:intron | 30.0% | |
| TAATAAGAAACCAGTTCGAA+AGG | + | chr7.4:1612872-1612891 | MS.gene22847:CDS | 30.0% | |
| TTATAGAGATCTTCATCAAC+AGG | - | chr7.4:1613506-1613525 | None:intergenic | 30.0% | |
| ! | TCTCATTCACCATAACATTT+TGG | - | chr7.4:1613346-1613365 | None:intergenic | 30.0% |
| !! | AAGGAAGTACCAAAATGTTA+TGG | + | chr7.4:1613334-1613353 | MS.gene22847:CDS | 30.0% |
| AAGGAAGCAAATGAACAAGA+AGG | + | chr7.4:1613439-1613458 | MS.gene22847:CDS | 35.0% | |
| AGTTGTCAAATACCAGCTTT+CGG | + | chr7.4:1612693-1612712 | MS.gene22847:CDS | 35.0% | |
| ATTCGTTACAGTTCTTCATC+TGG | + | chr7.4:1612783-1612802 | MS.gene22847:CDS | 35.0% | |
| TTCTTGTTCATTTGCTTCCT+TGG | - | chr7.4:1613440-1613459 | None:intergenic | 35.0% | |
| ! | AGATCTTCATCAACAGGTTT+TGG | - | chr7.4:1613500-1613519 | None:intergenic | 35.0% |
| ! | ATGTGATTGATGGTTGAGAA+AGG | + | chr7.4:1613168-1613187 | MS.gene22847:intron | 35.0% |
| ! | TTTGTTATGTGTAGGCAACA+AGG | + | chr7.4:1613303-1613322 | MS.gene22847:intron | 35.0% |
| AAATACCAGCTTTCGGAAAC+TGG | + | chr7.4:1612700-1612719 | MS.gene22847:CDS | 40.0% | |
| AATACCAGCTTTCGGAAACT+GGG | + | chr7.4:1612701-1612720 | MS.gene22847:CDS | 40.0% | |
| ATGAACATGAGAAAGCAGCA+AGG | + | chr7.4:1613389-1613408 | MS.gene22847:CDS | 40.0% | |
| ATGTGTAGGCAACAAGGAAT+AGG | + | chr7.4:1613309-1613328 | MS.gene22847:intron | 40.0% | |
| TGAACATGAGAAAGCAGCAA+GGG | + | chr7.4:1613390-1613409 | MS.gene22847:CDS | 40.0% | |
| TGTGTAGGCAACAAGGAATA+GGG | + | chr7.4:1613310-1613329 | MS.gene22847:intron | 40.0% | |
| ! | TTTGAAACTGCAAGACAAGC+TGG | + | chr7.4:1612756-1612775 | MS.gene22847:CDS | 40.0% |
| AAATCCCAGTTTCCGAAAGC+TGG | - | chr7.4:1612708-1612727 | None:intergenic | 45.0% | |
| ACGAGTCATGATGACCCATA+TGG | + | chr7.4:1613139-1613158 | MS.gene22847:intron | 45.0% | |
| AGCTACTTCGCACAACTAAG+AGG | + | chr7.4:1613537-1613556 | MS.gene22847:CDS | 45.0% | |
| AGGCAACAAGGAATAGGGAA+AGG | + | chr7.4:1613315-1613334 | MS.gene22847:intron | 45.0% | |
| GCTACTTCGCACAACTAAGA+GGG | + | chr7.4:1613538-1613557 | MS.gene22847:CDS | 45.0% | |
| GGGTTTCATTTCCAAGTGTC+TGG | + | chr7.4:1613678-1613697 | MS.gene22847:CDS | 45.0% | |
| GTGGTTGTTACCTTTCGAAC+TGG | - | chr7.4:1612885-1612904 | None:intergenic | 45.0% | |
| TTAGTTGTGCGAAGTAGCTC+AGG | - | chr7.4:1613536-1613555 | None:intergenic | 45.0% | |
| ! | ACGACGTAATGGAACATCCA+AGG | + | chr7.4:1613420-1613439 | MS.gene22847:CDS | 45.0% |
| ! | AGGGAAAGTGTACGACGTAA+TGG | + | chr7.4:1613409-1613428 | MS.gene22847:CDS | 45.0% |
| ! | GGAAGTCTCGTTGGTTGTAA+TGG | - | chr7.4:1613479-1613498 | None:intergenic | 45.0% |
| !!! | ACAGGTTTTGGAAGTCTCGT+TGG | - | chr7.4:1613488-1613507 | None:intergenic | 45.0% |
| CTTCGCACAACTAAGAGGGT+TGG | + | chr7.4:1613542-1613561 | MS.gene22847:intron | 50.0% | |
| GCTGCACGAACATGAACGTA+AGG | - | chr7.4:1612819-1612838 | None:intergenic | 50.0% | |
| TTGTGCGAAGTAGCTCAGGA+GGG | - | chr7.4:1613532-1613551 | None:intergenic | 50.0% | |
| GTTGTGCGAAGTAGCTCAGG+AGG | - | chr7.4:1613533-1613552 | None:intergenic | 55.0% | |
| AGCAGCAGGCACCAGACACT+TGG | - | chr7.4:1613692-1613711 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 1612576 | 1613723 | 1612576 | ID=MS.gene22847 |
| chr7.4 | mRNA | 1612576 | 1613723 | 1612576 | ID=MS.gene22847.t1;Parent=MS.gene22847 |
| chr7.4 | exon | 1612576 | 1612581 | 1612576 | ID=MS.gene22847.t1.exon1;Parent=MS.gene22847.t1 |
| chr7.4 | CDS | 1612576 | 1612581 | 1612576 | ID=cds.MS.gene22847.t1;Parent=MS.gene22847.t1 |
| chr7.4 | exon | 1612681 | 1612893 | 1612681 | ID=MS.gene22847.t1.exon2;Parent=MS.gene22847.t1 |
| chr7.4 | CDS | 1612681 | 1612893 | 1612681 | ID=cds.MS.gene22847.t1;Parent=MS.gene22847.t1 |
| chr7.4 | exon | 1613317 | 1613559 | 1613317 | ID=MS.gene22847.t1.exon3;Parent=MS.gene22847.t1 |
| chr7.4 | CDS | 1613317 | 1613559 | 1613317 | ID=cds.MS.gene22847.t1;Parent=MS.gene22847.t1 |
| chr7.4 | exon | 1613667 | 1613723 | 1613667 | ID=MS.gene22847.t1.exon4;Parent=MS.gene22847.t1 |
| chr7.4 | CDS | 1613667 | 1613723 | 1613667 | ID=cds.MS.gene22847.t1;Parent=MS.gene22847.t1 |
| Gene Sequence |
| Protein sequence |
