Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050592.t1 | RHN49490.1 | 84.4 | 173 | 18 | 3 | 2 | 172 | 3 | 168 | 9.50E-72 | 279.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050592.t1 | A0A396H898 | 84.4 | 173 | 18 | 3 | 2 | 172 | 3 | 168 | 6.9e-72 | 279.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050592 | MS.gene062188 | -0.801458 | 8.92E-49 | -1.69E-46 |
| MS.gene050592 | MS.gene07387 | 0.8064 | 8.38E-50 | -1.69E-46 |
| MS.gene050592 | MS.gene22847 | 0.986238 | 4.00E-166 | -1.69E-46 |
| MS.gene050592 | MS.gene29374 | -0.800104 | 1.69E-48 | -1.69E-46 |
| MS.gene050592 | MS.gene61957 | 0.809514 | 1.82E-50 | -1.69E-46 |
| MS.gene050592 | MS.gene85560 | 0.815739 | 7.92E-52 | -1.69E-46 |
| MS.gene050592 | MS.gene90786 | -0.813256 | 2.81E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050592.t1 | MTR_7g116610 | 91.716 | 169 | 5 | 3 | 6 | 172 | 3 | 164 | 7.61e-109 | 307 |
| MS.gene050592.t1 | MTR_8g011470 | 33.742 | 163 | 79 | 5 | 9 | 169 | 11 | 146 | 2.04e-18 | 77.4 |
| MS.gene050592.t1 | MTR_8g011470 | 36.842 | 152 | 60 | 6 | 9 | 155 | 11 | 131 | 4.84e-16 | 70.9 |
| MS.gene050592.t1 | MTR_7g052620 | 29.775 | 178 | 84 | 9 | 4 | 172 | 6 | 151 | 1.33e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050592.t1 | AT5G18310 | 34.911 | 169 | 94 | 5 | 5 | 169 | 11 | 167 | 1.62e-20 | 83.6 |
| MS.gene050592.t1 | AT5G48500 | 30.636 | 173 | 94 | 6 | 3 | 167 | 9 | 163 | 5.26e-14 | 66.6 |
| MS.gene050592.t1 | AT5G18310 | 31.579 | 152 | 88 | 5 | 22 | 169 | 1 | 140 | 1.18e-11 | 59.7 |
Find 35 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTCAAAGTATTGAGTTAT+TGG | 0.194110 | 7.3:-1517004 | None:intergenic |
| TCTCATTCACCATAACATTT+TGG | 0.210593 | 7.3:-1517602 | None:intergenic |
| TTCTTGTTCATTTGCTTCCT+TGG | 0.212136 | 7.3:-1517696 | None:intergenic |
| AGATCTTCATCAACAGGTTT+TGG | 0.271957 | 7.3:-1517756 | None:intergenic |
| AAATACCAGCTTTCGGAAAC+TGG | 0.279440 | 7.3:+1516963 | MS.gene050592:CDS |
| ATGTGTAGGCAACAAGGAAT+AGG | 0.352813 | 7.3:+1517568 | MS.gene050592:intron |
| TTATAGAGATCTTCATCAAC+AGG | 0.392468 | 7.3:-1517762 | None:intergenic |
| GGGTTTCATTTCCAAGTGTC+TGG | 0.396397 | 7.3:+1518169 | MS.gene050592:CDS |
| GGAAGTCTCGTTGGTTGTAA+TGG | 0.406598 | 7.3:-1517735 | None:intergenic |
| ATTCGTTACAGTTCTTCATC+TGG | 0.411701 | 7.3:+1517046 | MS.gene050592:CDS |
| AGTTGTCAAATACCAGCTTT+CGG | 0.450434 | 7.3:+1516956 | MS.gene050592:CDS |
| GTGGTTGTTACCTTTCGAAC+TGG | 0.453480 | 7.3:-1517145 | None:intergenic |
| AAGGAAGTACCAAAATGTTA+TGG | 0.457410 | 7.3:+1517593 | MS.gene050592:CDS |
| AGCTACTTCGCACAACTAAG+AGG | 0.477902 | 7.3:+1517796 | MS.gene050592:CDS |
| AAATCCCAGTTTCCGAAAGC+TGG | 0.479485 | 7.3:-1516968 | None:intergenic |
| TTAGTTGTGCGAAGTAGCTC+AGG | 0.482904 | 7.3:-1517792 | None:intergenic |
| TTTGAAACTGCAAGACAAGC+TGG | 0.494930 | 7.3:+1517019 | MS.gene050592:CDS |
| AGCAGCAGGCACCAGACACT+TGG | 0.496535 | 7.3:-1518180 | None:intergenic |
| AGGGAAAGTGTACGACGTAA+TGG | 0.509693 | 7.3:+1517668 | MS.gene050592:CDS |
| TGTGTAGGCAACAAGGAATA+GGG | 0.512863 | 7.3:+1517569 | MS.gene050592:intron |
| CTGCTGCTTGTGTTTCATGA+GGG | 0.515777 | 7.3:+1518195 | MS.gene050592:CDS |
| CCTGCTGCTTGTGTTTCATG+AGG | 0.531196 | 7.3:+1518194 | MS.gene050592:CDS |
| AATACCAGCTTTCGGAAACT+GGG | 0.543714 | 7.3:+1516964 | MS.gene050592:CDS |
| CCTCATGAAACACAAGCAGC+AGG | 0.582533 | 7.3:-1518194 | None:intergenic |
| TAATAAGAAACCAGTTCGAA+AGG | 0.596559 | 7.3:+1517135 | MS.gene050592:CDS |
| GCTGCACGAACATGAACGTA+AGG | 0.605636 | 7.3:-1517079 | None:intergenic |
| TTTGTTATGTGTAGGCAACA+AGG | 0.611013 | 7.3:+1517562 | MS.gene050592:intron |
| AAGGAAGCAAATGAACAAGA+AGG | 0.611603 | 7.3:+1517698 | MS.gene050592:CDS |
| TGAACATGAGAAAGCAGCAA+GGG | 0.621624 | 7.3:+1517649 | MS.gene050592:CDS |
| GTTGTGCGAAGTAGCTCAGG+AGG | 0.623117 | 7.3:-1517789 | None:intergenic |
| GCTACTTCGCACAACTAAGA+GGG | 0.636297 | 7.3:+1517797 | MS.gene050592:CDS |
| AGGCAACAAGGAATAGGGAA+AGG | 0.648026 | 7.3:+1517574 | MS.gene050592:intron |
| ATGAACATGAGAAAGCAGCA+AGG | 0.652015 | 7.3:+1517648 | MS.gene050592:CDS |
| ACGACGTAATGGAACATCCA+AGG | 0.662179 | 7.3:+1517679 | MS.gene050592:CDS |
| TTGTGCGAAGTAGCTCAGGA+GGG | 0.688459 | 7.3:-1517788 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAAATGTATAAATAAA+GGG | + | chr7.3:1517903-1517922 | MS.gene050592:intron | 10.0% |
| !! | TAAGAAAAATGTATAAATAA+AGG | + | chr7.3:1517902-1517921 | MS.gene050592:intron | 10.0% |
| !! | AGAAAAATGTATAAATAAAG+GGG | + | chr7.3:1517904-1517923 | MS.gene050592:intron | 15.0% |
| !! | ATTTAGTGTTATATTGTATT+GGG | + | chr7.3:1517968-1517987 | MS.gene050592:intron | 15.0% |
| !! | TATTTAGTGTTATATTGTAT+TGG | + | chr7.3:1517967-1517986 | MS.gene050592:intron | 15.0% |
| !!! | AGTAGTACAAAAATAATTTT+TGG | + | chr7.3:1517506-1517525 | MS.gene050592:intron | 15.0% |
| !!! | TATTTTTTTTATTTCCTAGT+GGG | + | chr7.3:1517478-1517497 | MS.gene050592:intron | 15.0% |
| !! | AAAGTGGAATATATTGTATT+GGG | + | chr7.3:1517927-1517946 | MS.gene050592:intron | 20.0% |
| !! | AAAGTTGTAAGGTTAAAATT+TGG | - | chr7.3:1517209-1517228 | None:intergenic | 20.0% |
| !! | AAGAACAAGAAAAAACAAAA+AGG | - | chr7.3:1517170-1517189 | None:intergenic | 20.0% |
| !! | AGTTGTATTGAAAATTGAAA+TGG | + | chr7.3:1518000-1518019 | MS.gene050592:intron | 20.0% |
| !! | ATTAACAACATTTCAAATCA+CGG | - | chr7.3:1518110-1518129 | None:intergenic | 20.0% |
| !! | CACTAGGAAATAAAAAAAAT+AGG | - | chr7.3:1517479-1517498 | None:intergenic | 20.0% |
| !! | GTTGTATTGAAAATTGAAAT+GGG | + | chr7.3:1518001-1518020 | MS.gene050592:intron | 20.0% |
| !!! | CTATTTTTTTTATTTCCTAG+TGG | + | chr7.3:1517477-1517496 | MS.gene050592:intron | 20.0% |
| !!! | GACACTTTTTTTTAGAAAAT+GGG | + | chr7.3:1518037-1518056 | MS.gene050592:intron | 20.0% |
| !!! | GTACAAAAATAATTTTTGGT+TGG | + | chr7.3:1517510-1517529 | MS.gene050592:intron | 20.0% |
| !!! | TAATGTTTGTTGTTGTTAAT+AGG | + | chr7.3:1516922-1516941 | MS.gene050592:intron | 20.0% |
| !!! | TTATATGATTTGTTATGTGT+AGG | + | chr7.3:1517554-1517573 | MS.gene050592:intron | 20.0% |
| ! | AACAAGAAAAAACAAAAAGG+TGG | - | chr7.3:1517167-1517186 | None:intergenic | 25.0% |
| ! | CAATGATTATGAAAGTTGTA+AGG | - | chr7.3:1517220-1517239 | None:intergenic | 25.0% |
| ! | CATCACATTATTAACCATAT+GGG | - | chr7.3:1517419-1517438 | None:intergenic | 25.0% |
| ! | GAAAGTGGAATATATTGTAT+TGG | + | chr7.3:1517926-1517945 | MS.gene050592:intron | 25.0% |
| ! | TTTGTGCAGAAAAAAATGTT+GGG | + | chr7.3:1518149-1518168 | MS.gene050592:intron | 25.0% |
| !! | TGAGTTAGATTTTTCATTTG+AGG | - | chr7.3:1517252-1517271 | None:intergenic | 25.0% |
| !!! | ACTATTTACTCTGAAATTTG+TGG | + | chr7.3:1517351-1517370 | MS.gene050592:intron | 25.0% |
| !!! | GGACACTTTTTTTTAGAAAA+TGG | + | chr7.3:1518036-1518055 | MS.gene050592:intron | 25.0% |
| !!! | GTTTCAAAGTATTGAGTTAT+TGG | - | chr7.3:1517007-1517026 | None:intergenic | 25.0% |
| !!! | TTTGTTGTTGTTAATAGGAA+TGG | + | chr7.3:1516927-1516946 | MS.gene050592:intron | 25.0% |
| AATAATGTGATGGTTGAGAA+AGG | + | chr7.3:1517427-1517446 | MS.gene050592:intron | 30.0% | |
| CCATATGGTTAATAATGTGA+TGG | + | chr7.3:1517417-1517436 | MS.gene050592:intron | 30.0% | |
| CCATCACATTATTAACCATA+TGG | - | chr7.3:1517420-1517439 | None:intergenic | 30.0% | |
| GTTTGTGCAGAAAAAAATGT+TGG | + | chr7.3:1518148-1518167 | MS.gene050592:intron | 30.0% | |
| TAATAAGAAACCAGTTCGAA+AGG | + | chr7.3:1517135-1517154 | MS.gene050592:CDS | 30.0% | |
| TGTATAAATAAAGGGGAAAG+TGG | + | chr7.3:1517911-1517930 | MS.gene050592:intron | 30.0% | |
| TTATAGAGATCTTCATCAAC+AGG | - | chr7.3:1517765-1517784 | None:intergenic | 30.0% | |
| ! | TCTCATTCACCATAACATTT+TGG | - | chr7.3:1517605-1517624 | None:intergenic | 30.0% |
| ! | TTGAAATGGGTCAGTTATTT+TGG | + | chr7.3:1518014-1518033 | MS.gene050592:intron | 30.0% |
| !! | AAGGAAGTACCAAAATGTTA+TGG | + | chr7.3:1517593-1517612 | MS.gene050592:CDS | 30.0% |
| !! | TGAAATGGGTCAGTTATTTT+GGG | + | chr7.3:1518015-1518034 | MS.gene050592:intron | 30.0% |
| AAGGAAGCAAATGAACAAGA+AGG | + | chr7.3:1517698-1517717 | MS.gene050592:CDS | 35.0% | |
| AGAAAATGGGTCAGTTATTC+CGG | + | chr7.3:1518050-1518069 | MS.gene050592:intron | 35.0% | |
| AGAGGTCAAATAAATTGGGA+CGG | - | chr7.3:1517863-1517882 | None:intergenic | 35.0% | |
| AGCCAAGAGGTCAAATAAAT+TGG | - | chr7.3:1517868-1517887 | None:intergenic | 35.0% | |
| AGTTGTCAAATACCAGCTTT+CGG | + | chr7.3:1516956-1516975 | MS.gene050592:CDS | 35.0% | |
| ATTCGTTACAGTTCTTCATC+TGG | + | chr7.3:1517046-1517065 | MS.gene050592:CDS | 35.0% | |
| GCCAAGAGGTCAAATAAATT+GGG | - | chr7.3:1517867-1517886 | None:intergenic | 35.0% | |
| TCCCAATTTATTTGACCTCT+TGG | + | chr7.3:1517863-1517882 | MS.gene050592:intron | 35.0% | |
| TTCTTGTTCATTTGCTTCCT+TGG | - | chr7.3:1517699-1517718 | None:intergenic | 35.0% | |
| ! | AGATCTTCATCAACAGGTTT+TGG | - | chr7.3:1517759-1517778 | None:intergenic | 35.0% |
| ! | TTTGTTATGTGTAGGCAACA+AGG | + | chr7.3:1517562-1517581 | MS.gene050592:intron | 35.0% |
| AAATACCAGCTTTCGGAAAC+TGG | + | chr7.3:1516963-1516982 | MS.gene050592:CDS | 40.0% | |
| AATACCAGCTTTCGGAAACT+GGG | + | chr7.3:1516964-1516983 | MS.gene050592:CDS | 40.0% | |
| ATGAACATGAGAAAGCAGCA+AGG | + | chr7.3:1517648-1517667 | MS.gene050592:CDS | 40.0% | |
| ATGTGTAGGCAACAAGGAAT+AGG | + | chr7.3:1517568-1517587 | MS.gene050592:intron | 40.0% | |
| GAAAATGGGTCAGTTATTCC+GGG | + | chr7.3:1518051-1518070 | MS.gene050592:intron | 40.0% | |
| GTCAAATAAATTGGGACGGA+GGG | - | chr7.3:1517859-1517878 | None:intergenic | 40.0% | |
| TGAACATGAGAAAGCAGCAA+GGG | + | chr7.3:1517649-1517668 | MS.gene050592:CDS | 40.0% | |
| TGTGTAGGCAACAAGGAATA+GGG | + | chr7.3:1517569-1517588 | MS.gene050592:intron | 40.0% | |
| ! | TTTGAAACTGCAAGACAAGC+TGG | + | chr7.3:1517019-1517038 | MS.gene050592:CDS | 40.0% |
| AAATCCCAGTTTCCGAAAGC+TGG | - | chr7.3:1516971-1516990 | None:intergenic | 45.0% | |
| ACGAGTCATGATGACCCATA+TGG | + | chr7.3:1517402-1517421 | MS.gene050592:intron | 45.0% | |
| AGCTACTTCGCACAACTAAG+AGG | + | chr7.3:1517796-1517815 | MS.gene050592:CDS | 45.0% | |
| AGGCAACAAGGAATAGGGAA+AGG | + | chr7.3:1517574-1517593 | MS.gene050592:intron | 45.0% | |
| GCTACTTCGCACAACTAAGA+GGG | + | chr7.3:1517797-1517816 | MS.gene050592:CDS | 45.0% | |
| GGGTTTCATTTCCAAGTGTC+TGG | + | chr7.3:1518169-1518188 | MS.gene050592:CDS | 45.0% | |
| GGTCAAATAAATTGGGACGG+AGG | - | chr7.3:1517860-1517879 | None:intergenic | 45.0% | |
| GTGGTTGTTACCTTTCGAAC+TGG | - | chr7.3:1517148-1517167 | None:intergenic | 45.0% | |
| TCTGTGTGAAATGAGCCAAG+AGG | - | chr7.3:1517881-1517900 | None:intergenic | 45.0% | |
| TGTACTACTACTAGCCCACT+AGG | - | chr7.3:1517495-1517514 | None:intergenic | 45.0% | |
| TTAGTTGTGCGAAGTAGCTC+AGG | - | chr7.3:1517795-1517814 | None:intergenic | 45.0% | |
| ! | ACGACGTAATGGAACATCCA+AGG | + | chr7.3:1517679-1517698 | MS.gene050592:CDS | 45.0% |
| ! | AGGGAAAGTGTACGACGTAA+TGG | + | chr7.3:1517668-1517687 | MS.gene050592:CDS | 45.0% |
| ! | GGAAGTCTCGTTGGTTGTAA+TGG | - | chr7.3:1517738-1517757 | None:intergenic | 45.0% |
| !!! | ACAGGTTTTGGAAGTCTCGT+TGG | - | chr7.3:1517747-1517766 | None:intergenic | 45.0% |
| AAATACTACTCCCTCCGTCC+CGG | - | chr7.3:1518072-1518091 | None:intergenic | 50.0% | |
| ATGGGTCAGTTATTCCGGGA+CGG | + | chr7.3:1518055-1518074 | MS.gene050592:intron | 50.0% | |
| CTTCGCACAACTAAGAGGGT+TGG | + | chr7.3:1517801-1517820 | MS.gene050592:intron | 50.0% | |
| GCTGCACGAACATGAACGTA+AGG | - | chr7.3:1517082-1517101 | None:intergenic | 50.0% | |
| TTGTGCGAAGTAGCTCAGGA+GGG | - | chr7.3:1517791-1517810 | None:intergenic | 50.0% | |
| GTCAGTTATTCCGGGACGGA+GGG | + | chr7.3:1518059-1518078 | MS.gene050592:intron | 55.0% | |
| GTTGTGCGAAGTAGCTCAGG+AGG | - | chr7.3:1517792-1517811 | None:intergenic | 55.0% | |
| AGCAGCAGGCACCAGACACT+TGG | - | chr7.3:1518183-1518202 | None:intergenic | 60.0% | |
| GGTCAGTTATTCCGGGACGG+AGG | + | chr7.3:1518058-1518077 | MS.gene050592:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 1516839 | 1518214 | 1516839 | ID=MS.gene050592 |
| chr7.3 | mRNA | 1516839 | 1518214 | 1516839 | ID=MS.gene050592.t1;Parent=MS.gene050592 |
| chr7.3 | exon | 1516839 | 1516844 | 1516839 | ID=MS.gene050592.t1.exon1;Parent=MS.gene050592.t1 |
| chr7.3 | CDS | 1516839 | 1516844 | 1516839 | ID=cds.MS.gene050592.t1;Parent=MS.gene050592.t1 |
| chr7.3 | exon | 1516944 | 1517156 | 1516944 | ID=MS.gene050592.t1.exon2;Parent=MS.gene050592.t1 |
| chr7.3 | CDS | 1516944 | 1517156 | 1516944 | ID=cds.MS.gene050592.t1;Parent=MS.gene050592.t1 |
| chr7.3 | exon | 1517576 | 1517818 | 1517576 | ID=MS.gene050592.t1.exon3;Parent=MS.gene050592.t1 |
| chr7.3 | CDS | 1517576 | 1517818 | 1517576 | ID=cds.MS.gene050592.t1;Parent=MS.gene050592.t1 |
| chr7.3 | exon | 1518158 | 1518214 | 1518158 | ID=MS.gene050592.t1.exon4;Parent=MS.gene050592.t1 |
| chr7.3 | CDS | 1518158 | 1518214 | 1518158 | ID=cds.MS.gene050592.t1;Parent=MS.gene050592.t1 |
| Gene Sequence |
| Protein sequence |
