Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23098.t1 | XP_003626080.1 | 89.2 | 277 | 15 | 3 | 2 | 278 | 1 | 262 | 5.00E-123 | 450.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23098.t1 | Q0JNI9 | 35.3 | 232 | 119 | 6 | 72 | 278 | 213 | 438 | 1.7e-15 | 84.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23098.t1 | G7KUN6 | 89.2 | 277 | 15 | 3 | 2 | 278 | 1 | 262 | 3.6e-123 | 450.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050829 | MS.gene23098 | 0.95642 | 3.08E-114 | -1.69E-46 |
| MS.gene050830 | MS.gene23098 | 0.852668 | 3.97E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTCATAATTTATCTGAA+AGG | + | 50588:8146-8165 | MS.gene23098:CDS | 15.0% |
| !! | AATTCATAATTTATCTGAAA+GGG | + | 50588:8147-8166 | MS.gene23098:CDS | 15.0% |
| ! | AACAAACGTAAAATAGCATA+TGG | - | 50588:7914-7933 | MS.gene23098:intergenic | 25.0% |
| ! | AATAAAGTTCAACTTCAACA+AGG | - | 50588:8388-8407 | MS.gene23098:intergenic | 25.0% |
| ! | CTTACATCATCACTTAAATA+TGG | - | 50588:7877-7896 | MS.gene23098:intergenic | 25.0% |
| ! | TGAAGAAACACAAGAAAATA+CGG | + | 50588:8066-8085 | MS.gene23098:CDS | 25.0% |
| !! | TATTTAAGTGATGATGTAAG+TGG | + | 50588:7877-7896 | MS.gene23098:intron | 25.0% |
| !!! | TGCTTTGTTTTTGTTATAGA+TGG | + | 50588:8444-8463 | MS.gene23098:intron | 25.0% |
| ACCAAAGTTTAGATCATTTG+AGG | - | 50588:7821-7840 | MS.gene23098:intergenic | 30.0% | |
| ACTTAAATATGGAGAGTCAT+CGG | - | 50588:7866-7885 | MS.gene23098:intergenic | 30.0% | |
| ATAATTTATCTGAAAGGGTG+AGG | + | 50588:8152-8171 | MS.gene23098:intron | 30.0% | |
| GGGTGTGAATATCAAAAAAA+TGG | + | 50588:7484-7503 | MS.gene23098:CDS | 30.0% | |
| GTGAGTACTATTCTTACTTT+GGG | - | 50588:7377-7396 | MS.gene23098:intergenic | 30.0% | |
| TCACATACCTTATTGCAATT+TGG | - | 50588:8330-8349 | MS.gene23098:intergenic | 30.0% | |
| TGTGAGTACTATTCTTACTT+TGG | - | 50588:7378-7397 | MS.gene23098:intergenic | 30.0% | |
| TTCATATGCAGAGTTTGATT+TGG | - | 50588:7650-7669 | MS.gene23098:intergenic | 30.0% | |
| TTTCTCTTTCCTCTAATTCT+AGG | - | 50588:7739-7758 | MS.gene23098:intergenic | 30.0% | |
| ! | TGGTGTTAGAAAATCATATG+AGG | + | 50588:7837-7856 | MS.gene23098:CDS | 30.0% |
| !! | CTTCTTTTGATTCTTTCTCT+TGG | - | 50588:7576-7595 | MS.gene23098:intergenic | 30.0% |
| AAAGAAAAGCCAGCTCATAA+TGG | + | 50588:8091-8110 | MS.gene23098:CDS | 35.0% | |
| AGCTTCAGAATGTTCATCAA+CGG | - | 50588:7695-7714 | MS.gene23098:intergenic | 35.0% | |
| AGTACTATTCTTACTTTGGG+AGG | - | 50588:7374-7393 | MS.gene23098:intergenic | 35.0% | |
| ATCAAGCTTCGTAAGAATGA+TGG | - | 50588:8025-8044 | MS.gene23098:intergenic | 35.0% | |
| GCCTCAAATGATCTAAACTT+TGG | + | 50588:7817-7836 | MS.gene23098:CDS | 35.0% | |
| GCTCATACCAAATTGCAATA+AGG | + | 50588:8320-8339 | MS.gene23098:CDS | 35.0% | |
| TCACAGAAACAGTACTTGAT+GGG | + | 50588:7463-7482 | MS.gene23098:CDS | 35.0% | |
| TCCTCTCAAAAAACCAAGAT+TGG | + | 50588:7414-7433 | MS.gene23098:CDS | 35.0% | |
| ! | TAATTCTAGGTGCATTGGTA+TGG | - | 50588:7726-7745 | MS.gene23098:intergenic | 35.0% |
| !! | GTTTGGTTTGAATCCAATCT+TGG | - | 50588:7430-7449 | MS.gene23098:intergenic | 35.0% |
| !!! | TCCAATCTTGGTTTTTTGAG+AGG | - | 50588:7418-7437 | MS.gene23098:intergenic | 35.0% |
| ACCAATGCACCTAGAATTAG+AGG | + | 50588:7727-7746 | MS.gene23098:CDS | 40.0% | |
| ACTCTGTTTCCATTATGAGC+TGG | - | 50588:8103-8122 | MS.gene23098:intergenic | 40.0% | |
| ATTGAAACTGTGTCTGTGTC+TGG | - | 50588:7977-7996 | MS.gene23098:intergenic | 40.0% | |
| CACAGAAACAGTACTTGATG+GGG | + | 50588:7464-7483 | MS.gene23098:CDS | 40.0% | |
| CTCACAGAAACAGTACTTGA+TGG | + | 50588:7462-7481 | MS.gene23098:CDS | 40.0% | |
| GGAATGAAAATCACTGTGAG+AGG | - | 50588:7353-7372 | MS.gene23098:intergenic | 40.0% | |
| TCAAGTGAGCATACAGAAGA+TGG | - | 50588:7796-7815 | MS.gene23098:intergenic | 40.0% | |
| TCAGAATGTTCATCAACGGT+TGG | - | 50588:7691-7710 | MS.gene23098:intergenic | 40.0% | |
| TCAGAGATGACAAGAAGCAA+TGG | + | 50588:7319-7338 | MS.gene23098:CDS | 40.0% | |
| TCCTCTAATTCTAGGTGCAT+TGG | - | 50588:7731-7750 | MS.gene23098:intergenic | 40.0% | |
| TTACTATGAAGCACGAACAC+TGG | + | 50588:7939-7958 | MS.gene23098:intron | 40.0% | |
| !! | GTGAGTTTGTTGTTGCAGTT+TGG | - | 50588:7447-7466 | MS.gene23098:intergenic | 40.0% |
| !! | GTTGATTTGAGAAACACTGC+AGG | - | 50588:7538-7557 | MS.gene23098:intergenic | 40.0% |
| AATCACTGTGAGAGGAACCT+AGG | - | 50588:7345-7364 | MS.gene23098:intergenic | 45.0% | |
| ACAGAGTCAAGAGAAGCTAC+AGG | + | 50588:8116-8135 | MS.gene23098:CDS | 45.0% | |
| ATGACAAGAAGCAATGGCCT+AGG | + | 50588:7325-7344 | MS.gene23098:CDS | 45.0% | |
| ! | AGGTGCATTGGTATGGCTAG+AGG | - | 50588:7719-7738 | MS.gene23098:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50588 | gene | 7297 | 8528 | 7297 | ID=MS.gene23098 |
| 50588 | mRNA | 7297 | 8528 | 7297 | ID=MS.gene23098.t1;Parent=MS.gene23098 |
| 50588 | exon | 7297 | 7891 | 7297 | ID=MS.gene23098.t1.exon1;Parent=MS.gene23098.t1 |
| 50588 | CDS | 7297 | 7891 | 7297 | ID=cds.MS.gene23098.t1;Parent=MS.gene23098.t1 |
| 50588 | exon | 8061 | 8168 | 8061 | ID=MS.gene23098.t1.exon2;Parent=MS.gene23098.t1 |
| 50588 | CDS | 8061 | 8168 | 8061 | ID=cds.MS.gene23098.t1;Parent=MS.gene23098.t1 |
| 50588 | exon | 8276 | 8341 | 8276 | ID=MS.gene23098.t1.exon3;Parent=MS.gene23098.t1 |
| 50588 | CDS | 8276 | 8341 | 8276 | ID=cds.MS.gene23098.t1;Parent=MS.gene23098.t1 |
| 50588 | exon | 8463 | 8528 | 8463 | ID=MS.gene23098.t1.exon4;Parent=MS.gene23098.t1 |
| 50588 | CDS | 8463 | 8528 | 8463 | ID=cds.MS.gene23098.t1;Parent=MS.gene23098.t1 |
| Gene Sequence |
| Protein sequence |
