Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23263.t1 | XP_003606916.1 | 88.9 | 45 | 5 | 0 | 1 | 45 | 1 | 45 | 6.60E-13 | 82.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23263.t1 | P49310 | 66.7 | 45 | 15 | 0 | 1 | 45 | 1 | 45 | 6.9e-11 | 67.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23263.t1 | G7JG73 | 88.9 | 45 | 5 | 0 | 1 | 45 | 1 | 45 | 4.8e-13 | 82.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050702 | MS.gene23263 | -0.847044 | 1.48E-59 | -1.69E-46 |
| MS.gene058551 | MS.gene23263 | 0.844609 | 6.80E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23263.t1 | MTR_4g070140 | 88.889 | 45 | 5 | 0 | 1 | 45 | 1 | 45 | 3.42e-22 | 83.6 |
| MS.gene23263.t1 | MTR_4g070190 | 71.111 | 45 | 13 | 0 | 1 | 45 | 1 | 45 | 5.05e-18 | 72.4 |
| MS.gene23263.t1 | MTR_4g070080 | 69.048 | 42 | 13 | 0 | 4 | 45 | 2 | 43 | 5.19e-17 | 68.9 |
| MS.gene23263.t1 | MTR_4g070080 | 69.048 | 42 | 13 | 0 | 4 | 45 | 2 | 43 | 1.00e-16 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23263.t1 | AT2G21660 | 62.222 | 45 | 17 | 0 | 1 | 45 | 1 | 45 | 2.57e-15 | 65.1 |
| MS.gene23263.t1 | AT2G21660 | 62.222 | 45 | 17 | 0 | 1 | 45 | 1 | 45 | 3.53e-15 | 65.1 |
| MS.gene23263.t1 | AT4G39260 | 59.524 | 42 | 17 | 0 | 4 | 45 | 2 | 43 | 1.48e-13 | 58.9 |
| MS.gene23263.t1 | AT4G39260 | 59.524 | 42 | 17 | 0 | 4 | 45 | 2 | 43 | 2.07e-13 | 58.9 |
| MS.gene23263.t1 | AT4G39260 | 59.524 | 42 | 17 | 0 | 4 | 45 | 2 | 43 | 2.65e-13 | 59.3 |
| MS.gene23263.t1 | AT4G39260 | 59.524 | 42 | 17 | 0 | 4 | 45 | 2 | 43 | 5.97e-13 | 59.3 |
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCGTCGGTGGTCTTGCT+TGG | 0.353153 | 4.2:+36599075 | MS.gene23263:CDS |
| GAGCAAGCCTTCTCTAAGTT+TGG | 0.374142 | 4.2:+36599119 | MS.gene23263:CDS |
| ATCAGAATCCGCACGGACCT+TGG | 0.384806 | 4.2:-36599156 | None:intergenic |
| CTTCGTCGGTGGTCTTGCTT+GGG | 0.385202 | 4.2:+36599076 | MS.gene23263:CDS |
| GGCCACCGATAGCCAAGCTC+TGG | 0.439871 | 4.2:+36599097 | MS.gene23263:CDS |
| GGTCCGTGCGGATTCTGATT+CGG | 0.446825 | 4.2:+36599160 | MS.gene23263:CDS |
| AGAAGGCTTGCTCCAGAGCT+TGG | 0.478074 | 4.2:-36599109 | None:intergenic |
| CAAGACCACCGACGAAGCAC+CGG | 0.514431 | 4.2:-36599070 | None:intergenic |
| TGGTGAAATCACTGATTCCA+AGG | 0.553599 | 4.2:+36599139 | MS.gene23263:CDS |
| TTGCTCCAGAGCTTGGCTAT+CGG | 0.556107 | 4.2:-36599102 | None:intergenic |
| CTCCAGAGCTTGGCTATCGG+TGG | 0.571940 | 4.2:-36599099 | None:intergenic |
| GATTTCACCAAACTTAGAGA+AGG | 0.575852 | 4.2:-36599126 | None:intergenic |
| GTTGAGTACCGGTGCTTCGT+CGG | 0.588309 | 4.2:+36599062 | MS.gene23263:CDS |
| GAGTACCGGTGCTTCGTCGG+TGG | 0.614835 | 4.2:+36599065 | MS.gene23263:CDS |
| GTTCTTCTGATGTTGAGTAC+CGG | 0.622558 | 4.2:+36599051 | MS.gene23263:CDS |
| CACTGATTCCAAGGTCCGTG+CGG | 0.648788 | 4.2:+36599148 | MS.gene23263:CDS |
| AATCCGAATCAGAATCCGCA+CGG | 0.698903 | 4.2:-36599163 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GATTTCACCAAACTTAGAGA+AGG | - | chr4.2:36599129-36599148 | None:intergenic | 35.0% | |
| GTTCTTCTGATGTTGAGTAC+CGG | + | chr4.2:36599051-36599070 | MS.gene23263:CDS | 40.0% | |
| !! | TGGTGAAATCACTGATTCCA+AGG | + | chr4.2:36599139-36599158 | MS.gene23263:CDS | 40.0% |
| AATCCGAATCAGAATCCGCA+CGG | - | chr4.2:36599166-36599185 | None:intergenic | 45.0% | |
| GAGCAAGCCTTCTCTAAGTT+TGG | + | chr4.2:36599119-36599138 | MS.gene23263:CDS | 45.0% | |
| TTGCTCCAGAGCTTGGCTAT+CGG | - | chr4.2:36599105-36599124 | None:intergenic | 50.0% | |
| ATCAGAATCCGCACGGACCT+TGG | - | chr4.2:36599159-36599178 | None:intergenic | 55.0% | |
| GGTCCGTGCGGATTCTGATT+CGG | + | chr4.2:36599160-36599179 | MS.gene23263:CDS | 55.0% | |
| ! | AGAAGGCTTGCTCCAGAGCT+TGG | - | chr4.2:36599112-36599131 | None:intergenic | 55.0% |
| ! | CTTCGTCGGTGGTCTTGCTT+GGG | + | chr4.2:36599076-36599095 | MS.gene23263:CDS | 55.0% |
| !! | CACTGATTCCAAGGTCCGTG+CGG | + | chr4.2:36599148-36599167 | MS.gene23263:CDS | 55.0% |
| !! | GTTGAGTACCGGTGCTTCGT+CGG | + | chr4.2:36599062-36599081 | MS.gene23263:CDS | 55.0% |
| CAAGACCACCGACGAAGCAC+CGG | - | chr4.2:36599073-36599092 | None:intergenic | 60.0% | |
| CTCCAGAGCTTGGCTATCGG+TGG | - | chr4.2:36599102-36599121 | None:intergenic | 60.0% | |
| ! | GCTTCGTCGGTGGTCTTGCT+TGG | + | chr4.2:36599075-36599094 | MS.gene23263:CDS | 60.0% |
| GGCCACCGATAGCCAAGCTC+TGG | + | chr4.2:36599097-36599116 | MS.gene23263:CDS | 65.0% | |
| !! | GAGTACCGGTGCTTCGTCGG+TGG | + | chr4.2:36599065-36599084 | MS.gene23263:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 36599047 | 36599202 | 36599047 | ID=MS.gene23263 |
| chr4.2 | mRNA | 36599047 | 36599202 | 36599047 | ID=MS.gene23263.t1;Parent=MS.gene23263 |
| chr4.2 | exon | 36599047 | 36599202 | 36599047 | ID=MS.gene23263.t1.exon1;Parent=MS.gene23263.t1 |
| chr4.2 | CDS | 36599047 | 36599202 | 36599047 | ID=cds.MS.gene23263.t1;Parent=MS.gene23263.t1 |
| Gene Sequence |
| Protein sequence |
