AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058551

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058551.t1 MTR_8g105590 98.898 635 7 0 1 635 1 635 0.0 1297
MS.gene058551.t1 MTR_2g036510 67.016 573 187 1 59 629 37 609 0.0 811
MS.gene058551.t1 MTR_4g007980 27.139 409 254 15 238 611 32 431 1.68e-30 125
MS.gene058551.t1 MTR_2g095980 47.273 110 49 1 63 172 158 258 3.31e-22 102
MS.gene058551.t1 MTR_2g095980 35.455 110 62 1 63 172 441 541 5.81e-13 72.8
MS.gene058551.t1 MTR_2g095980 47.273 110 49 1 63 172 158 258 3.58e-22 102
MS.gene058551.t1 MTR_2g095980 35.455 110 62 1 63 172 441 541 5.63e-13 72.8
MS.gene058551.t1 MTR_4g061610 43.636 110 53 1 63 172 165 265 2.88e-21 99.4
MS.gene058551.t1 MTR_4g061610 33.077 130 76 2 63 190 459 579 4.64e-14 76.3
MS.gene058551.t1 MTR_8g070530 35.450 189 99 5 59 246 124 290 3.95e-19 92.4
MS.gene058551.t1 MTR_8g070530 35.455 110 62 1 63 172 412 512 3.63e-12 70.1
MS.gene058551.t1 MTR_1g084120 29.012 324 197 15 311 629 22 317 4.95e-18 88.6
MS.gene058551.t1 MTR_4g109680 29.352 293 156 18 303 572 3 267 2.90e-15 79.7
MS.gene058551.t1 MTR_4g109680 32.000 175 109 6 303 472 115 284 1.44e-12 71.2
MS.gene058551.t1 MTR_1g090740 27.317 205 140 5 291 491 115 314 1.10e-14 77.4
MS.gene058551.t1 MTR_1g090740 25.305 328 204 14 303 614 15 317 5.55e-12 68.9
MS.gene058551.t1 MTR_1g492850 30.721 319 177 20 316 615 20 313 2.77e-14 76.3
MS.gene058551.t1 MTR_1g492850 29.924 264 154 11 365 618 17 259 1.03e-13 74.3
MS.gene058551.t1 MTR_1g492850 30.721 319 177 20 316 615 20 313 3.34e-14 75.9
MS.gene058551.t1 MTR_1g492850 29.924 264 154 11 365 618 17 259 1.52e-13 73.6
MS.gene058551.t1 MTR_4g132120 29.470 302 174 18 320 611 282 554 6.09e-13 72.8
MS.gene058551.t1 MTR_2g095980 35.455 110 62 1 63 172 186 286 7.79e-13 72.0
MS.gene058551.t1 MTR_2g087530 28.638 213 147 4 303 512 42 252 1.37e-12 71.6
MS.gene058551.t1 MTR_2g087530 28.638 213 147 4 303 512 154 364 1.75e-12 71.2
MS.gene058551.t1 MTR_7g113200 27.500 200 132 6 298 491 275 467 7.66e-12 68.9
MS.gene058551.t1 MTR_7g113200 26.087 345 210 17 303 630 174 490 7.12e-11 65.9
MS.gene058551.t1 MTR_1g089370 25.346 217 157 4 303 517 86 299 8.36e-12 68.9
MS.gene058551.t1 MTR_1g089370 26.493 268 159 11 364 611 26 275 8.74e-11 65.5
MS.gene058551.t1 MTR_7g113200 28.931 159 107 4 298 455 275 428 1.15e-11 67.8
MS.gene058551.t1 MTR_5g038420 34.545 110 63 1 63 172 252 352 1.22e-11 67.4
MS.gene058551.t1 MTR_2g019860 25.610 328 198 16 303 619 76 368 8.66e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058551.t1 AT1G68050 79.615 623 117 4 15 635 5 619 0.0 1019
MS.gene058551.t1 AT5G57360 65.124 605 195 4 33 629 10 606 0.0 803
MS.gene058551.t1 AT5G57360 64.874 595 193 4 33 619 10 596 0.0 787
MS.gene058551.t1 AT2G18915 62.176 579 196 5 59 629 42 605 0.0 739
MS.gene058551.t1 AT2G18915 60.929 581 190 7 59 629 42 595 0.0 715
MS.gene058551.t1 AT1G51550 26.667 405 252 16 243 611 41 436 3.76e-28 119
MS.gene058551.t1 AT5G58140 43.860 114 55 1 59 172 130 234 1.49e-20 96.7
MS.gene058551.t1 AT5G58140 33.871 124 71 2 63 184 390 504 2.14e-12 70.9
MS.gene058551.t1 AT5G58140 43.860 114 55 1 59 172 130 234 1.97e-20 96.7
MS.gene058551.t1 AT5G58140 33.871 124 71 2 63 184 390 504 2.67e-12 70.5
MS.gene058551.t1 AT5G58140 43.860 114 55 1 59 172 130 234 2.69e-20 96.3
MS.gene058551.t1 AT5G58140 33.871 124 71 2 63 184 390 504 3.43e-12 70.1
MS.gene058551.t1 AT5G58140 43.860 114 55 1 59 172 130 234 2.69e-20 96.3
MS.gene058551.t1 AT5G58140 33.871 124 71 2 63 184 390 504 3.43e-12 70.1
MS.gene058551.t1 AT5G58140 43.860 114 55 1 59 172 130 234 2.69e-20 96.3
MS.gene058551.t1 AT5G58140 33.871 124 71 2 63 184 390 504 3.43e-12 70.1
MS.gene058551.t1 AT5G58140 43.860 114 55 1 59 172 130 234 2.69e-20 96.3
MS.gene058551.t1 AT5G58140 33.871 124 71 2 63 184 390 504 3.43e-12 70.1
MS.gene058551.t1 AT5G58140 43.860 114 55 1 59 172 130 234 2.69e-20 96.3
MS.gene058551.t1 AT5G58140 33.871 124 71 2 63 184 390 504 3.43e-12 70.1
MS.gene058551.t1 AT3G45780 40.909 110 56 1 63 172 198 298 2.61e-17 86.7
MS.gene058551.t1 AT3G45780 32.331 133 78 2 63 195 476 596 1.48e-12 71.6
MS.gene058551.t1 AT3G45780 40.909 110 56 1 63 172 198 298 2.61e-17 86.7
MS.gene058551.t1 AT3G45780 32.331 133 78 2 63 195 476 596 1.48e-12 71.6
MS.gene058551.t1 AT1G18610 32.056 287 155 18 356 625 3 266 6.72e-17 84.7
MS.gene058551.t1 AT1G18610 28.205 312 163 19 287 571 44 321 9.10e-15 78.2
MS.gene058551.t1 AT1G18610 32.056 287 155 18 356 625 3 266 6.72e-17 84.7
MS.gene058551.t1 AT1G18610 28.205 312 163 19 287 571 44 321 9.10e-15 78.2
MS.gene058551.t1 AT2G36360 30.741 270 153 11 365 618 17 268 6.78e-17 84.7
MS.gene058551.t1 AT1G03445 28.679 265 164 11 303 557 86 335 2.30e-16 83.6
MS.gene058551.t1 AT1G03445 29.020 255 157 10 303 547 86 326 2.37e-16 83.6
MS.gene058551.t1 AT2G36360 30.189 265 152 11 365 618 17 259 9.41e-16 80.9
MS.gene058551.t1 AT2G36360 28.527 319 186 17 315 615 19 313 7.23e-12 68.6
MS.gene058551.t1 AT2G36360 30.189 265 152 11 365 618 17 259 9.95e-16 80.9
MS.gene058551.t1 AT2G36360 28.527 319 186 17 315 615 19 313 6.75e-12 68.6
MS.gene058551.t1 AT2G36360 30.189 265 152 11 365 618 17 259 1.15e-15 80.5
MS.gene058551.t1 AT2G36360 28.527 319 186 17 315 615 19 313 8.64e-12 68.6
MS.gene058551.t1 AT2G36360 30.189 265 152 11 365 618 17 259 1.15e-15 80.5
MS.gene058551.t1 AT2G36360 28.527 319 186 17 315 615 19 313 8.64e-12 68.6
MS.gene058551.t1 AT2G02710 33.636 110 64 1 63 172 260 360 1.81e-14 76.3
MS.gene058551.t1 AT2G02710 30.464 151 81 6 61 196 36 177 7.44e-11 65.1
MS.gene058551.t1 AT1G74150 28.571 266 152 14 317 572 27 264 4.74e-13 72.4
MS.gene058551.t1 AT1G74150 28.571 266 152 14 317 572 27 264 4.85e-13 72.4
MS.gene058551.t1 AT5G04420 27.751 209 137 6 306 505 143 346 5.16e-13 72.4
MS.gene058551.t1 AT5G04420 27.751 209 137 6 306 505 143 346 5.16e-13 72.4
MS.gene058551.t1 AT5G04420 27.751 209 137 6 306 505 143 346 5.16e-13 72.4
MS.gene058551.t1 AT1G74150 28.571 266 152 14 317 572 27 264 5.23e-13 72.4
MS.gene058551.t1 AT2G02710 33.036 112 64 2 63 172 260 362 7.50e-13 71.2
MS.gene058551.t1 AT2G02710 30.464 151 81 6 61 196 36 177 7.92e-11 65.1
MS.gene058551.t1 AT1G08420 28.638 213 147 4 303 512 175 385 1.01e-12 72.0
MS.gene058551.t1 AT2G27210 28.169 213 148 4 303 512 164 374 1.17e-12 72.0
MS.gene058551.t1 AT1G08420 29.146 199 136 4 303 498 175 371 2.69e-12 70.9
MS.gene058551.t1 AT2G27210 28.643 199 137 4 303 498 164 360 3.08e-12 70.5
MS.gene058551.t1 AT5G27630 29.911 224 133 11 289 496 206 421 5.75e-12 69.3
MS.gene058551.t1 AT5G27630 29.911 224 133 11 289 496 206 421 7.22e-12 68.9
MS.gene058551.t1 AT5G27630 29.911 224 133 11 289 496 206 421 7.36e-12 68.9
MS.gene058551.t1 AT5G27630 29.911 224 133 11 289 496 206 421 1.08e-11 68.2
MS.gene058551.t1 AT2G02710 32.743 113 66 3 61 172 36 139 6.37e-11 64.7
MS.gene058551.t1 AT2G02710 30.464 151 81 6 61 196 36 177 6.45e-11 65.1

Find 181 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATAGGCTTGTATTATT+TGG 0.172159 4.4:+1875469 MS.gene058551:CDS
TGTGGGAGGGTTATTATATT+TGG 0.207983 4.4:+1876141 MS.gene058551:CDS
GGGCACTCACTCTCAGTTTA+TGG 0.213464 4.4:+1875919 MS.gene058551:CDS
CATCGATTGGTTCTGTTTGC+AGG 0.229475 4.4:+1875227 MS.gene058551:CDS
CTTCATGGACTCCTCCTTCT+AGG 0.236476 4.4:+1875893 MS.gene058551:CDS
CACAATTGAAGGCTCTAAAT+TGG 0.241941 4.4:+1875774 MS.gene058551:CDS
TCTCTTGCTTAAACAGTTCT+TGG 0.261051 4.4:+1875614 MS.gene058551:CDS
ACACTAACCCTTTGCCATTC+TGG 0.266477 4.4:-1875556 None:intergenic
GTGCTTGTGCTGCAGGAAAT+AGG 0.284638 4.4:+1875455 MS.gene058551:CDS
TGGACTCCTCCTTCTAGGTT+AGG 0.286313 4.4:+1875898 MS.gene058551:CDS
CTATGACCCCAAGCAAATTT+AGG 0.308735 4.4:-1876264 None:intergenic
ACCGTCTCAGCTATTTCTAT+TGG 0.311844 4.4:+1876188 MS.gene058551:CDS
TGTTTGCTTCGGAAAAGATT+TGG 0.312883 4.4:-1875006 None:intergenic
CAGCCGGTTCTTGCAATATA+GGG 0.313877 4.4:+1874790 MS.gene058551:intron
AAAGTCTTTGAAATCTCAAC+TGG 0.330143 4.4:+1873876 MS.gene058551:CDS
ATATTTGGAGGTTCCATTGC+AGG 0.333382 4.4:+1876156 MS.gene058551:CDS
GCAGCCGGTTCTTGCAATAT+AGG 0.345908 4.4:+1874789 MS.gene058551:intron
ATCCTCCCCGCCCGGCCGAT+GGG 0.346004 4.4:+1875582 MS.gene058551:CDS
AGCTCAGAGGCGGCATCCTT+TGG 0.346721 4.4:+1874820 MS.gene058551:CDS
TGATGTGTTTGTGCTTGATT+TGG 0.349642 4.4:+1875678 MS.gene058551:CDS
ACGACCCCAACCTAACTTCT+TGG 0.349674 4.4:-1875344 None:intergenic
GGTTGGAGGGACTAGAGTTT+TGG 0.350009 4.4:+1876296 MS.gene058551:CDS
TGTGGCCCCATCGGCCGGGC+GGG 0.357098 4.4:-1875588 None:intergenic
CTACTCTTGAGGCTGTTTGC+TGG 0.369379 4.4:+1875383 MS.gene058551:CDS
GTGGAGGATCCTCAATGTTC+CGG 0.370289 4.4:+1876233 MS.gene058551:CDS
ATGCAAGTCTCTCTGAAAAC+TGG 0.371445 4.4:-1875052 None:intergenic
CGCCGCCTCTTCCGAGATCT+TGG 0.372949 4.4:+1875740 MS.gene058551:CDS
GTTGGGTAGAAGAAAAGTTC+TGG 0.374493 4.4:-1873789 None:intergenic
GGAGAGAAATTCCAACTTCA+TGG 0.384945 4.4:+1875878 MS.gene058551:CDS
ATGAAGCCCTTGGTCGTAAC+TGG 0.387595 4.4:+1873910 MS.gene058551:CDS
CACGTCGGTTTCTCTTCGGC+AGG 0.391277 4.4:-1876213 None:intergenic
GGAACCACCTAAATTTGCTT+GGG 0.393741 4.4:+1876257 MS.gene058551:CDS
GAGTGTGTGGCCCCATCGGC+CGG 0.399734 4.4:-1875593 None:intergenic
TGGACATACCGGGGAGGAAT+GGG 0.400686 4.4:+1876326 MS.gene058551:CDS
TTGGTGTTGGGTGGACATAC+CGG 0.402125 4.4:+1876315 MS.gene058551:CDS
TGGAGGATCCTCAATGTTCC+GGG 0.414753 4.4:+1876234 MS.gene058551:CDS
TGGTGTTGGGTGGACATACC+GGG 0.415143 4.4:+1876316 MS.gene058551:CDS
ACAAAAGATGTTGGTGTTGT+TGG 0.417028 4.4:-1873807 None:intergenic
GGAGTCGTGACACACATAAT+AGG 0.418405 4.4:+1874980 MS.gene058551:CDS
TATCCTCCCCGCCCGGCCGA+TGG 0.425086 4.4:+1875581 MS.gene058551:CDS
GAACTGATGACCAAGAAGTT+AGG 0.427716 4.4:+1875334 MS.gene058551:CDS
CACAGCTCATGCACAATTGA+AGG 0.428020 4.4:+1875763 MS.gene058551:CDS
AATTTCAGTGCTTGTGCTGC+AGG 0.430336 4.4:+1875448 MS.gene058551:CDS
AGTGAGTGCCCTAACCTAGA+AGG 0.434298 4.4:-1875907 None:intergenic
TTGCAAAGAGTGGACACTTA+AGG 0.435187 4.4:+1875971 MS.gene058551:CDS
GCTCGTTCTTTGTCAATTGA+CGG 0.436187 4.4:-1875255 None:intergenic
GCTAAATATTCTCCCAAAAG+TGG 0.437183 4.4:+1875094 MS.gene058551:CDS
TCAGCTTTCTGATGAAGTCT+TGG 0.437833 4.4:+1875165 MS.gene058551:CDS
AGGACAAAGATTCTCATGTT+TGG 0.440239 4.4:+1875943 MS.gene058551:CDS
GTTCAAGTCTATGTTTGCTT+CGG 0.440669 4.4:-1875017 None:intergenic
GGGAGGGTTATTATATTTGG+AGG 0.441072 4.4:+1876144 MS.gene058551:CDS
GGACTCCTCCTTCTAGGTTA+GGG 0.442999 4.4:+1875899 MS.gene058551:CDS
GGTTCATAATCAAAATCAAA+AGG 0.443531 4.4:+1873674 MS.gene058551:CDS
ACTCCATCATCATCATGAAT+TGG 0.445762 4.4:-1874962 None:intergenic
ACTTTGTTGACATAAATGAT+TGG 0.446777 4.4:-1873858 None:intergenic
GTGGCCCCATCGGCCGGGCG+GGG 0.447496 4.4:-1875587 None:intergenic
GGTCATAGCACTTGTGTGGT+TGG 0.452644 4.4:+1876279 MS.gene058551:CDS
TGACAAAGAACGAGCACGTT+AGG 0.454258 4.4:+1875263 MS.gene058551:CDS
AGGCTCTAAATTGGTTGTCT+CGG 0.461684 4.4:+1875783 MS.gene058551:CDS
GGCTCTAAATTGGTTGTCTC+GGG 0.463717 4.4:+1875784 MS.gene058551:CDS
TCCTCCCCGCCCGGCCGATG+GGG 0.463860 4.4:+1875583 MS.gene058551:CDS
CCGAGGGATGTTGCATCGAT+TGG 0.463890 4.4:+1875214 MS.gene058551:CDS
AATAGGCTTGTATTATTTGG+AGG 0.465306 4.4:+1875472 MS.gene058551:CDS
AAGCCTAAGTCTGTTGACTA+AGG 0.465707 4.4:-1874936 None:intergenic
GAACGAGCACGTTAGGAAGA+TGG 0.469272 4.4:+1875270 MS.gene058551:CDS
CTTGTATTATTTGGAGGTGA+AGG 0.470299 4.4:+1875478 MS.gene058551:CDS
GATGACCAAGAAGTTAGGTT+GGG 0.472628 4.4:+1875339 MS.gene058551:CDS
GCCCCATCGGCCGGGCGGGG+AGG 0.477243 4.4:-1875584 None:intergenic
TAGGTTGGGGTCGTCTGACT+CGG 0.478884 4.4:+1875353 MS.gene058551:CDS
CAAAAGATGTTGGTGTTGTT+GGG 0.483296 4.4:-1873806 None:intergenic
GGGAACCACCTAAATTTGCT+TGG 0.486452 4.4:+1876256 MS.gene058551:CDS
CACATCTCTTCGCGCTGCCG+TGG 0.487345 4.4:-1875133 None:intergenic
CTCCCTTAGTCAACAGACTT+AGG 0.488483 4.4:+1874933 MS.gene058551:CDS
CATAGCACTTGTGTGGTTGG+AGG 0.488750 4.4:+1876282 MS.gene058551:CDS
CCTCCACGTCGGTTTCTCTT+CGG 0.490496 4.4:-1876217 None:intergenic
ATTAGAAGATGTCTTGAAGA+AGG 0.500324 4.4:+1874866 MS.gene058551:CDS
GTGGACATACCGGGGAGGAA+TGG 0.501214 4.4:+1876325 MS.gene058551:CDS
CTAATCTCCGACACAACGAC+TGG 0.501353 4.4:-1874848 None:intergenic
GTTGGAATTTCTCTCCATGT+AGG 0.501983 4.4:-1875871 None:intergenic
GGATGCCGCCTCTGAGCTCT+AGG 0.505139 4.4:-1874815 None:intergenic
CTTCTGAATTTCAGAAAGGA+TGG 0.505201 4.4:+1874908 MS.gene058551:CDS
TTGCAGTAAGCATGCCTTGT+GGG 0.506427 4.4:+1876124 MS.gene058551:CDS
TGAAATTCAGAAGTTCCCCT+TGG 0.507034 4.4:-1874898 None:intergenic
GATCCCTATATTGCAAGAAC+CGG 0.508385 4.4:-1874793 None:intergenic
TTTGGTGGATGTGGTAGGCA+AGG 0.511551 4.4:+1875646 MS.gene058551:CDS
GAAGGAGGAGTCCATGAAGT+TGG 0.513595 4.4:-1875889 None:intergenic
CAGTAAGCATGCCTTGTGGG+AGG 0.517765 4.4:+1876127 MS.gene058551:CDS
ACAAAGATTCTCATGTTTGG+TGG 0.518883 4.4:+1875946 MS.gene058551:CDS
GGAACTTCTGAATTTCAGAA+AGG 0.531302 4.4:+1874904 MS.gene058551:CDS
CCGACATGGAAGGAAGTCTT+CGG 0.531353 4.4:+1875712 MS.gene058551:CDS
AGATGGTTTGTCAAAACGCA+TGG 0.532902 4.4:+1875287 MS.gene058551:CDS
CATTCTAGTTGCCTCCATTG+TGG 0.534438 4.4:-1876039 None:intergenic
GCAAATTTAGGTGGTTCCCC+CGG 0.536214 4.4:-1876252 None:intergenic
CAAAGAAGTGACAGGTACAT+TGG 0.538138 4.4:+1875312 MS.gene058551:CDS
ATAGCACTTGTGTGGTTGGA+GGG 0.538751 4.4:+1876283 MS.gene058551:CDS
AAGAGAGTGTGTGGCCCCAT+CGG 0.538839 4.4:-1875597 None:intergenic
TCGGGCGGCTGCACAGATGC+CGG 0.544982 4.4:+1875802 MS.gene058551:CDS
GTTCATAATCAAAATCAAAA+GGG 0.549520 4.4:+1873675 MS.gene058551:CDS
GGACACTTAAGGTTAAGATC+AGG 0.550094 4.4:+1875982 MS.gene058551:CDS
CGATTGGTTCTGTTTGCAGG+AGG 0.551211 4.4:+1875230 MS.gene058551:CDS
AGGAGTGGATATGCAGCCTA+TGG 0.551520 4.4:+1875498 MS.gene058551:CDS
CTAGAATGTAGTGCATTCAC+AGG 0.551583 4.4:+1876054 MS.gene058551:CDS
ATCTTACCAGTTACGACCAA+GGG 0.552243 4.4:-1873916 None:intergenic
AAGAAGGTATTGAGTTCCAA+GGG 0.553691 4.4:+1874882 MS.gene058551:CDS
TATCGTGCTCATGAAGCCCT+TGG 0.555109 4.4:+1873900 MS.gene058551:CDS
TCAGAGGCGGCATCCTTTGG+TGG 0.557577 4.4:+1874823 MS.gene058551:CDS
CATAATCAAAATCAAAAGGG+TGG 0.561242 4.4:+1873678 MS.gene058551:CDS
AAAAGTTACAGTTGGAGGCG+GGG 0.563457 4.4:+1875408 MS.gene058551:CDS
AGTCTCTCTGAAAACTGGAT+AGG 0.564618 4.4:-1875047 None:intergenic
ACGCCAATTCATGATGATGA+TGG 0.567655 4.4:+1874959 MS.gene058551:CDS
CTGTGCCAAGATCTCGGAAG+AGG 0.567792 4.4:-1875745 None:intergenic
CTAAGAATCCAGAATGGCAA+AGG 0.567914 4.4:+1875548 MS.gene058551:CDS
ACGTCGGTTTCTCTTCGGCA+GGG 0.569618 4.4:-1876212 None:intergenic
AGCCTAAGTCTGTTGACTAA+GGG 0.572540 4.4:-1874935 None:intergenic
TGATGACCAAGAAGTTAGGT+TGG 0.573837 4.4:+1875338 MS.gene058551:CDS
AGTGTGTGGCCCCATCGGCC+GGG 0.576130 4.4:-1875592 None:intergenic
GGTGGATCCAGTCGTTGTGT+CGG 0.576728 4.4:+1874841 MS.gene058551:CDS
TGCTCAACAGCCGACATGGA+AGG 0.576956 4.4:+1875702 MS.gene058551:CDS
CCGAAGACTTCCTTCCATGT+CGG 0.579808 4.4:-1875712 None:intergenic
AGAACAAAAGTATCATCCAT+AGG 0.580180 4.4:-1875514 None:intergenic
TCCAATAGAAATAGCTGAGA+CGG 0.580190 4.4:-1876189 None:intergenic
GATGGTTTGTCAAAACGCAT+GGG 0.583838 4.4:+1875288 MS.gene058551:CDS
ATAATCAAAATCAAAAGGGT+GGG 0.584258 4.4:+1873679 MS.gene058551:CDS
CCAATCGATGCAACATCCCT+CGG 0.585601 4.4:-1875214 None:intergenic
TGCCAAGATCTCGGAAGAGG+CGG 0.585827 4.4:-1875742 None:intergenic
AGTAAGCATGCCTTGTGGGA+GGG 0.586759 4.4:+1876128 MS.gene058551:CDS
CGGTGAATGAAGACCTGCAA+TGG 0.588794 4.4:-1876169 None:intergenic
GTGTGGCCCCATCGGCCGGG+CGG 0.590319 4.4:-1875589 None:intergenic
CATGAGCTGTGCCAAGATCT+CGG 0.590774 4.4:-1875751 None:intergenic
TAAGAATCCAGAATGGCAAA+GGG 0.592387 4.4:+1875549 MS.gene058551:CDS
AGGTTGGGGTCGTCTGACTC+GGG 0.592442 4.4:+1875354 MS.gene058551:CDS
GAAAATTATTGTACACTCCA+CGG 0.593713 4.4:+1875116 MS.gene058551:CDS
TGAGTTGCATGAGTTGTGCT+TGG 0.594676 4.4:+1876356 MS.gene058551:CDS
TGGATGCTCAACAGCCGACA+TGG 0.596002 4.4:+1875698 MS.gene058551:CDS
GGTGTTGGGTGGACATACCG+GGG 0.599502 4.4:+1876317 MS.gene058551:CDS
TTTGGTGGACTTGCAAAGAG+TGG 0.599541 4.4:+1875961 MS.gene058551:CDS
ATTCAGCACCCATTCCTCCC+CGG 0.599838 4.4:-1876334 None:intergenic
ACATGATCTAGTCGAGGAGG+AGG 0.604406 4.4:-1876102 None:intergenic
TCTAAATTGGTTGTCTCGGG+CGG 0.604939 4.4:+1875787 MS.gene058551:CDS
GAAGAAGGTATTGAGTTCCA+AGG 0.605857 4.4:+1874881 MS.gene058551:CDS
ATTATTTGGAGGTGAAGGAG+TGG 0.606129 4.4:+1875483 MS.gene058551:CDS
GCATGGGGCAAAGAAGTGAC+AGG 0.615126 4.4:+1875304 MS.gene058551:CDS
TCGGGAGCTTACTACTCTTG+AGG 0.615782 4.4:+1875372 MS.gene058551:CDS
GCACTGAAATTGCAGCGTGA+AGG 0.616623 4.4:-1875436 None:intergenic
ATGACCAAGAAGTTAGGTTG+GGG 0.617169 4.4:+1875340 MS.gene058551:CDS
CTTAAGGTTAAGATCAGGTG+AGG 0.617307 4.4:+1875987 MS.gene058551:CDS
ACTCACTCTCAGTTTATGGT+AGG 0.619329 4.4:+1875923 MS.gene058551:CDS
GAGTGCCCTAACCTAGAAGG+AGG 0.621134 4.4:-1875904 None:intergenic
TTGGGGTCATAGCACTTGTG+TGG 0.623428 4.4:+1876275 MS.gene058551:CDS
AGTGTGATATCCTCCCCGCC+CGG 0.623636 4.4:+1875574 MS.gene058551:CDS
TCTGATACAACAAAAGATGT+TGG 0.624603 4.4:-1873816 None:intergenic
TGTGTCACTAAGAAGTACTC+CGG 0.630445 4.4:-1875821 None:intergenic
AATCTTACCAGTTACGACCA+AGG 0.632469 4.4:-1873917 None:intergenic
TGTTTAAGCAAGAGAGTGTG+TGG 0.633821 4.4:-1875606 None:intergenic
TTGATGCTAAGAATCCAGAA+TGG 0.634155 4.4:+1875542 MS.gene058551:CDS
GAACCACCTAAATTTGCTTG+GGG 0.636271 4.4:+1876258 MS.gene058551:CDS
GGAGGATCCTCAATGTTCCG+GGG 0.639537 4.4:+1876235 MS.gene058551:CDS
TGACCCCAAGCAAATTTAGG+TGG 0.642462 4.4:-1876261 None:intergenic
ATTGTCACGCTTGACACCGA+GGG 0.643189 4.4:+1875198 MS.gene058551:CDS
TCACAATAGATAATCCTACA+TGG 0.646440 4.4:+1875857 MS.gene058551:CDS
CCGAAGAGAAACCGACGTGG+AGG 0.652010 4.4:+1876217 MS.gene058551:CDS
ATGTAGGATTATCTATTGTG+AGG 0.656684 4.4:-1875855 None:intergenic
AAGCTGAACAACCACAATGG+AGG 0.657091 4.4:+1876028 MS.gene058551:CDS
AATATAATAACCCTCCCACA+AGG 0.660973 4.4:-1876138 None:intergenic
CGGCAGCGCGAAGAGATGTG+TGG 0.661003 4.4:+1875136 MS.gene058551:CDS
ACAACGACTGGATCCACCAA+AGG 0.663685 4.4:-1874836 None:intergenic
GAGGATCCTCAATGTTCCGG+GGG 0.669777 4.4:+1876236 MS.gene058551:CDS
ATGGTTTGTCAAAACGCATG+GGG 0.675604 4.4:+1875289 MS.gene058551:CDS
TAGAAGCTGAACAACCACAA+TGG 0.675759 4.4:+1876025 MS.gene058551:CDS
AAGTCTTTGAAATCTCAACT+GGG 0.680191 4.4:+1873877 MS.gene058551:CDS
ACTGCAACATGATCTAGTCG+AGG 0.685428 4.4:-1876108 None:intergenic
GCAACATGATCTAGTCGAGG+AGG 0.692964 4.4:-1876105 None:intergenic
GGGATCCTAGAGCTCAGAGG+CGG 0.695636 4.4:+1874810 MS.gene058551:CDS
TGAAAACTGGATAGGAAACA+CGG 0.696786 4.4:-1875039 None:intergenic
GTTGCAGTAAGCATGCCTTG+TGG 0.698959 4.4:+1876123 MS.gene058551:CDS
CTGCCGAAGAGAAACCGACG+TGG 0.709608 4.4:+1876214 MS.gene058551:CDS
TATTGTCACGCTTGACACCG+AGG 0.711160 4.4:+1875197 MS.gene058551:CDS
ATAGGGATCCTAGAGCTCAG+AGG 0.719677 4.4:+1874807 MS.gene058551:CDS
GTTGGGTGGACATACCGGGG+AGG 0.725055 4.4:+1876320 MS.gene058551:CDS
AGAAGGTATTGAGTTCCAAG+GGG 0.731475 4.4:+1874883 MS.gene058551:CDS
ACATTGAGGATCCTCCACGT+CGG 0.754895 4.4:-1876228 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAAATTTGAGAAAT+AGG - chr4.4:1874432-1874451 None:intergenic 10.0%
!!! TTATCTTTTTTATTGATTTA+AGG + chr4.4:1874153-1874172 MS.gene058551:intron 10.0%
!! AAAGAAATATCTTGTAAAAA+AGG - chr4.4:1874702-1874721 None:intergenic 15.0%
!! ACTTTCCATAATAATAATTA+AGG + chr4.4:1874542-1874561 MS.gene058551:intron 15.0%
!! ATGAACCTTAATTATTATTA+TGG - chr4.4:1874550-1874569 None:intergenic 15.0%
!! TTATTTATTATCCAATAAGA+AGG - chr4.4:1874389-1874408 None:intergenic 15.0%
!!! TTCTTTTAGATATTTTGATT+TGG + chr4.4:1874716-1874735 MS.gene058551:intron 15.0%
!! AATACCAAATATAACCAAAA+AGG - chr4.4:1874673-1874692 None:intergenic 20.0%
!! GTTCATAATCAAAATCAAAA+GGG + chr4.4:1873675-1873694 MS.gene058551:CDS 20.0%
!! TTTGCAAAATGAAACAAAAT+TGG - chr4.4:1874318-1874337 None:intergenic 20.0%
!!! AAATAAACCCTTTTATCAAA+CGG + chr4.4:1874240-1874259 MS.gene058551:intron 20.0%
!!! AAGGGTTTATTTTTAGTTTA+AGG - chr4.4:1874232-1874251 None:intergenic 20.0%
!!! AAGTTTATTTTCCATTCTAT+TGG + chr4.4:1874468-1874487 MS.gene058551:intron 20.0%
!!! ATTTTTAGTTTAAGGAAAGT+GGG - chr4.4:1874224-1874243 None:intergenic 20.0%
!!! CTTTTTTATTGATTTAAGGT+TGG + chr4.4:1874157-1874176 MS.gene058551:intron 20.0%
!!! TACAATAATTTTCCACTTTT+GGG - chr4.4:1875109-1875128 None:intergenic 20.0%
!!! TATTTTTAGTTTAAGGAAAG+TGG - chr4.4:1874225-1874244 None:intergenic 20.0%
! AAAATGTTAAGCCAATAGAA+TGG - chr4.4:1874482-1874501 None:intergenic 25.0%
! ACTTTGTTGACATAAATGAT+TGG - chr4.4:1873861-1873880 None:intergenic 25.0%
! ATAATCAAAATCAAAAGGGT+GGG + chr4.4:1873679-1873698 MS.gene058551:CDS 25.0%
! GGAATCAAAATAAGAAAGAA+TGG - chr4.4:1874358-1874377 None:intergenic 25.0%
! GGTTCATAATCAAAATCAAA+AGG + chr4.4:1873674-1873693 MS.gene058551:CDS 25.0%
!! ATTTTCACCGTTTGATAAAA+GGG - chr4.4:1874250-1874269 None:intergenic 25.0%
!! GAATGGATTCTATAAAAATG+TGG - chr4.4:1874341-1874360 None:intergenic 25.0%
!! GTACAATAATTTTCCACTTT+TGG - chr4.4:1875110-1875129 None:intergenic 25.0%
!! TCTTGCATCATAATTTTGTT+GGG + chr4.4:1873950-1873969 MS.gene058551:intron 25.0%
!! TTCTTGCATCATAATTTTGT+TGG + chr4.4:1873949-1873968 MS.gene058551:intron 25.0%
!!! AAGAAAAGTTCTGGTTTTAA+TGG - chr4.4:1873783-1873802 None:intergenic 25.0%
!!! AGAAATTCTAAAGCAATTAG+AGG - chr4.4:1874762-1874781 None:intergenic 25.0%
AATAGGCTTGTATTATTTGG+AGG + chr4.4:1875472-1875491 MS.gene058551:CDS 30.0%
ACTCTCATAGAATCAATGAT+AGG + chr4.4:1874640-1874659 MS.gene058551:intron 30.0%
ATGTAGGATTATCTATTGTG+AGG - chr4.4:1875858-1875877 None:intergenic 30.0%
ATTAGAAGATGTCTTGAAGA+AGG + chr4.4:1874866-1874885 MS.gene058551:CDS 30.0%
CAACATAGTATAGATCTGAA+AGG - chr4.4:1874282-1874301 None:intergenic 30.0%
CATAATCAAAATCAAAAGGG+TGG + chr4.4:1873678-1873697 MS.gene058551:CDS 30.0%
CTCTCATAGAATCAATGATA+GGG + chr4.4:1874641-1874660 MS.gene058551:intron 30.0%
GAAAATTATTGTACACTCCA+CGG + chr4.4:1875116-1875135 MS.gene058551:CDS 30.0%
GGAAATAGGCTTGTATTATT+TGG + chr4.4:1875469-1875488 MS.gene058551:CDS 30.0%
TAAAAACGAAGATGAAGAAG+AGG + chr4.4:1873653-1873672 MS.gene058551:CDS 30.0%
TAGTATAGATCTGAAAGGAA+TGG - chr4.4:1874277-1874296 None:intergenic 30.0%
TCACAATAGATAATCCTACA+TGG + chr4.4:1875857-1875876 MS.gene058551:CDS 30.0%
TCTGATACAACAAAAGATGT+TGG - chr4.4:1873819-1873838 None:intergenic 30.0%
! ATACTTCCATGACATAGTTT+TGG + chr4.4:1873976-1873995 MS.gene058551:intron 30.0%
! CATTTTCACCGTTTGATAAA+AGG - chr4.4:1874251-1874270 None:intergenic 30.0%
! CTTGCATCATAATTTTGTTG+GGG + chr4.4:1873951-1873970 MS.gene058551:intron 30.0%
!! AAAGTCTTTGAAATCTCAAC+TGG + chr4.4:1873876-1873895 MS.gene058551:CDS 30.0%
!! AAAGTGCTACTCATTTTGTT+GGG + chr4.4:1874128-1874147 MS.gene058551:intron 30.0%
!! AAGTCTTTGAAATCTCAACT+GGG + chr4.4:1873877-1873896 MS.gene058551:CDS 30.0%
!! AGAACAAAAGTATCATCCAT+AGG - chr4.4:1875517-1875536 None:intergenic 30.0%
!! ATTTTGACAACATGGTTACT+TGG + chr4.4:1874497-1874516 MS.gene058551:intron 30.0%
!!! CTGTCCTTTTTGGTTATATT+TGG + chr4.4:1874666-1874685 MS.gene058551:intron 30.0%
AAAAAAGTTACAGTTGGAGG+CGG + chr4.4:1875406-1875425 MS.gene058551:CDS 35.0%
AATATAATAACCCTCCCACA+AGG - chr4.4:1876141-1876160 None:intergenic 35.0%
ACAAAGATTCTCATGTTTGG+TGG + chr4.4:1875946-1875965 MS.gene058551:CDS 35.0%
ACTCCATCATCATCATGAAT+TGG - chr4.4:1874965-1874984 None:intergenic 35.0%
AGGACAAAGATTCTCATGTT+TGG + chr4.4:1875943-1875962 MS.gene058551:CDS 35.0%
ATCCAATAAGAAGGAAGCTA+GGG - chr4.4:1874380-1874399 None:intergenic 35.0%
CAACAAAATGAGTAGCACTT+TGG - chr4.4:1874129-1874148 None:intergenic 35.0%
CTTCTGAATTTCAGAAAGGA+TGG + chr4.4:1874908-1874927 MS.gene058551:CDS 35.0%
CTTGTATTATTTGGAGGTGA+AGG + chr4.4:1875478-1875497 MS.gene058551:CDS 35.0%
GCTAAATATTCTCCCAAAAG+TGG + chr4.4:1875094-1875113 MS.gene058551:CDS 35.0%
GGAACTTCTGAATTTCAGAA+AGG + chr4.4:1874904-1874923 MS.gene058551:CDS 35.0%
GTTCAAGTCTATGTTTGCTT+CGG - chr4.4:1875020-1875039 None:intergenic 35.0%
TAAGAATCCAGAATGGCAAA+GGG + chr4.4:1875549-1875568 MS.gene058551:CDS 35.0%
TATCCAATAAGAAGGAAGCT+AGG - chr4.4:1874381-1874400 None:intergenic 35.0%
TCCAATAGAAATAGCTGAGA+CGG - chr4.4:1876192-1876211 None:intergenic 35.0%
TCTCTTGCTTAAACAGTTCT+TGG + chr4.4:1875614-1875633 MS.gene058551:CDS 35.0%
TGAAAACTGGATAGGAAACA+CGG - chr4.4:1875042-1875061 None:intergenic 35.0%
TGATGTGTTTGTGCTTGATT+TGG + chr4.4:1875678-1875697 MS.gene058551:CDS 35.0%
TGCTGGAAAAAAGTTACAGT+TGG + chr4.4:1875400-1875419 MS.gene058551:CDS 35.0%
TGGAAAAAAGTTACAGTTGG+AGG + chr4.4:1875403-1875422 MS.gene058551:CDS 35.0%
TGTGGGAGGGTTATTATATT+TGG + chr4.4:1876141-1876160 MS.gene058551:CDS 35.0%
TGTTTGCTTCGGAAAAGATT+TGG - chr4.4:1875009-1875028 None:intergenic 35.0%
TTGATGCTAAGAATCCAGAA+TGG + chr4.4:1875542-1875561 MS.gene058551:CDS 35.0%
!! AAGAAGGTATTGAGTTCCAA+GGG + chr4.4:1874882-1874901 MS.gene058551:CDS 35.0%
!! AAGTGCTACTCATTTTGTTG+GGG + chr4.4:1874129-1874148 MS.gene058551:intron 35.0%
!! ACAAAAGATGTTGGTGTTGT+TGG - chr4.4:1873810-1873829 None:intergenic 35.0%
!! AGTTCTTGGCTAGTTGTTTT+TGG + chr4.4:1875628-1875647 MS.gene058551:CDS 35.0%
!! CAAAAGATGTTGGTGTTGTT+GGG - chr4.4:1873809-1873828 None:intergenic 35.0%
!! CAAAGTGCTACTCATTTTGT+TGG + chr4.4:1874127-1874146 MS.gene058551:intron 35.0%
!! CACAATTGAAGGCTCTAAAT+TGG + chr4.4:1875774-1875793 MS.gene058551:CDS 35.0%
!! GAATTTCTTTTGTTTGCAGC+CGG + chr4.4:1874774-1874793 MS.gene058551:intron 35.0%
!! GGCTTAACATTTTGACAACA+TGG + chr4.4:1874489-1874508 MS.gene058551:intron 35.0%
AAAAAGTTACAGTTGGAGGC+GGG + chr4.4:1875407-1875426 MS.gene058551:CDS 40.0%
AATCTTACCAGTTACGACCA+AGG - chr4.4:1873920-1873939 None:intergenic 40.0%
ACTCACTCTCAGTTTATGGT+AGG + chr4.4:1875923-1875942 MS.gene058551:CDS 40.0%
ATATTTGGAGGTTCCATTGC+AGG + chr4.4:1876156-1876175 MS.gene058551:CDS 40.0%
ATCTTACCAGTTACGACCAA+GGG - chr4.4:1873919-1873938 None:intergenic 40.0%
ATGGCACCAAAACTATGTCA+TGG - chr4.4:1873985-1874004 None:intergenic 40.0%
ATTATTTGGAGGTGAAGGAG+TGG + chr4.4:1875483-1875502 MS.gene058551:CDS 40.0%
CAAAGAAGTGACAGGTACAT+TGG + chr4.4:1875312-1875331 MS.gene058551:CDS 40.0%
CACACATCCACACACATTAA+TGG - chr4.4:1874031-1874050 None:intergenic 40.0%
CTAAGAATCCAGAATGGCAA+AGG + chr4.4:1875548-1875567 MS.gene058551:CDS 40.0%
CTAGAATGTAGTGCATTCAC+AGG + chr4.4:1876054-1876073 MS.gene058551:CDS 40.0%
CTATGACCCCAAGCAAATTT+AGG - chr4.4:1876267-1876286 None:intergenic 40.0%
CTTAAGGTTAAGATCAGGTG+AGG + chr4.4:1875987-1876006 MS.gene058551:CDS 40.0%
GAACTGATGACCAAGAAGTT+AGG + chr4.4:1875334-1875353 MS.gene058551:CDS 40.0%
GATCCCTATATTGCAAGAAC+CGG - chr4.4:1874796-1874815 None:intergenic 40.0%
GATGACCAAGAAGTTAGGTT+GGG + chr4.4:1875339-1875358 MS.gene058551:CDS 40.0%
GCTCGTTCTTTGTCAATTGA+CGG - chr4.4:1875258-1875277 None:intergenic 40.0%
GGACACTTAAGGTTAAGATC+AGG + chr4.4:1875982-1876001 MS.gene058551:CDS 40.0%
GGAGAGAAATTCCAACTTCA+TGG + chr4.4:1875878-1875897 MS.gene058551:CDS 40.0%
GGGAGGGTTATTATATTTGG+AGG + chr4.4:1876144-1876163 MS.gene058551:CDS 40.0%
GTTGGAATTTCTCTCCATGT+AGG - chr4.4:1875874-1875893 None:intergenic 40.0%
GTTGGGTAGAAGAAAAGTTC+TGG - chr4.4:1873792-1873811 None:intergenic 40.0%
TAATGTGTGTGGATGTGTGT+GGG + chr4.4:1874032-1874051 MS.gene058551:intron 40.0%
TAGAAGCTGAACAACCACAA+TGG + chr4.4:1876025-1876044 MS.gene058551:CDS 40.0%
TCAGCTTTCTGATGAAGTCT+TGG + chr4.4:1875165-1875184 MS.gene058551:CDS 40.0%
TCCAATAAGAAGGAAGCTAG+GGG - chr4.4:1874379-1874398 None:intergenic 40.0%
TGAAATTCAGAAGTTCCCCT+TGG - chr4.4:1874901-1874920 None:intergenic 40.0%
TGATGACCAAGAAGTTAGGT+TGG + chr4.4:1875338-1875357 MS.gene058551:CDS 40.0%
TTAATGTGTGTGGATGTGTG+TGG + chr4.4:1874031-1874050 MS.gene058551:intron 40.0%
TTGCAAAGAGTGGACACTTA+AGG + chr4.4:1875971-1875990 MS.gene058551:CDS 40.0%
! AAGCCTAAGTCTGTTGACTA+AGG - chr4.4:1874939-1874958 None:intergenic 40.0%
! ACGCCAATTCATGATGATGA+TGG + chr4.4:1874959-1874978 MS.gene058551:CDS 40.0%
! AGATGGTTTGTCAAAACGCA+TGG + chr4.4:1875287-1875306 MS.gene058551:CDS 40.0%
! AGCCTAAGTCTGTTGACTAA+GGG - chr4.4:1874938-1874957 None:intergenic 40.0%
! AGTCTCTCTGAAAACTGGAT+AGG - chr4.4:1875050-1875069 None:intergenic 40.0%
! ATGACCAAGAAGTTAGGTTG+GGG + chr4.4:1875340-1875359 MS.gene058551:CDS 40.0%
! ATGCAAGTCTCTCTGAAAAC+TGG - chr4.4:1875055-1875074 None:intergenic 40.0%
! ATGGTTTGTCAAAACGCATG+GGG + chr4.4:1875289-1875308 MS.gene058551:CDS 40.0%
! GAACCACCTAAATTTGCTTG+GGG + chr4.4:1876258-1876277 MS.gene058551:CDS 40.0%
! GATGGTTTGTCAAAACGCAT+GGG + chr4.4:1875288-1875307 MS.gene058551:CDS 40.0%
! GGAACCACCTAAATTTGCTT+GGG + chr4.4:1876257-1876276 MS.gene058551:CDS 40.0%
! TGATAGGGAACTGTCCTTTT+TGG + chr4.4:1874656-1874675 MS.gene058551:intron 40.0%
! TGTTTAAGCAAGAGAGTGTG+TGG - chr4.4:1875609-1875628 None:intergenic 40.0%
!! ACCGTCTCAGCTATTTCTAT+TGG + chr4.4:1876188-1876207 MS.gene058551:CDS 40.0%
!! AGAAGGTATTGAGTTCCAAG+GGG + chr4.4:1874883-1874902 MS.gene058551:CDS 40.0%
!! AGGCTCTAAATTGGTTGTCT+CGG + chr4.4:1875783-1875802 MS.gene058551:CDS 40.0%
!! GAAGAAGGTATTGAGTTCCA+AGG + chr4.4:1874881-1874900 MS.gene058551:CDS 40.0%
!! TGTGTCACTAAGAAGTACTC+CGG - chr4.4:1875824-1875843 None:intergenic 40.0%
!!! CTAGTTGTTTTTGGTGGATG+TGG + chr4.4:1875637-1875656 MS.gene058551:CDS 40.0%
!!! TCTTGGCTAGTTGTTTTTGG+TGG + chr4.4:1875631-1875650 MS.gene058551:CDS 40.0%
!!! TGACATAGTTTTGGTGCCAT+TGG + chr4.4:1873985-1874004 MS.gene058551:intron 40.0%
!!! TTGTTTTTGGTGGATGTGGT+AGG + chr4.4:1875641-1875660 MS.gene058551:CDS 40.0%
AAAAGTTACAGTTGGAGGCG+GGG + chr4.4:1875408-1875427 MS.gene058551:CDS 45.0%
AAGCTGAACAACCACAATGG+AGG + chr4.4:1876028-1876047 MS.gene058551:CDS 45.0%
AATGTGTGTGGATGTGTGTG+GGG + chr4.4:1874033-1874052 MS.gene058551:intron 45.0%
AATTTCAGTGCTTGTGCTGC+AGG + chr4.4:1875448-1875467 MS.gene058551:CDS 45.0%
ACACTAACCCTTTGCCATTC+TGG - chr4.4:1875559-1875578 None:intergenic 45.0%
ACTGCAACATGATCTAGTCG+AGG - chr4.4:1876111-1876130 None:intergenic 45.0%
ATAGCACTTGTGTGGTTGGA+GGG + chr4.4:1876283-1876302 MS.gene058551:CDS 45.0%
ATCCACACACATTAATGGCC+AGG - chr4.4:1874026-1874045 None:intergenic 45.0%
ATCCTGGCCATTAATGTGTG+TGG + chr4.4:1874021-1874040 MS.gene058551:intron 45.0%
CACAGCTCATGCACAATTGA+AGG + chr4.4:1875763-1875782 MS.gene058551:CDS 45.0%
CTCCCTTAGTCAACAGACTT+AGG + chr4.4:1874933-1874952 MS.gene058551:CDS 45.0%
GGAGTCGTGACACACATAAT+AGG + chr4.4:1874980-1874999 MS.gene058551:CDS 45.0%
TCCCCTAGCTTCCTTCTTAT+TGG + chr4.4:1874375-1874394 MS.gene058551:intron 45.0%
TCTAAATTGGTTGTCTCGGG+CGG + chr4.4:1875787-1875806 MS.gene058551:CDS 45.0%
TGACAAAGAACGAGCACGTT+AGG + chr4.4:1875263-1875282 MS.gene058551:CDS 45.0%
TGACCCCAAGCAAATTTAGG+TGG - chr4.4:1876264-1876283 None:intergenic 45.0%
TGAGTTGCATGAGTTGTGCT+TGG + chr4.4:1876356-1876375 MS.gene058551:CDS 45.0%
TTGCAGTAAGCATGCCTTGT+GGG + chr4.4:1876124-1876143 MS.gene058551:CDS 45.0%
! CAGCCGGTTCTTGCAATATA+GGG + chr4.4:1874790-1874809 MS.gene058551:intron 45.0%
! CATTCTAGTTGCCTCCATTG+TGG - chr4.4:1876042-1876061 None:intergenic 45.0%
! GGGAACCACCTAAATTTGCT+TGG + chr4.4:1876256-1876275 MS.gene058551:CDS 45.0%
!! AGGGACTAGAGTTTTGGTGT+TGG + chr4.4:1876302-1876321 MS.gene058551:CDS 45.0%
!! CATCGATTGGTTCTGTTTGC+AGG + chr4.4:1875227-1875246 MS.gene058551:CDS 45.0%
!! GGCTCTAAATTGGTTGTCTC+GGG + chr4.4:1875784-1875803 MS.gene058551:CDS 45.0%
!! TTTGGTGGACTTGCAAAGAG+TGG + chr4.4:1875961-1875980 MS.gene058551:CDS 45.0%
!!! ACTAGAGTTTTGGTGTTGGG+TGG + chr4.4:1876306-1876325 MS.gene058551:CDS 45.0%
!!! GGGACTAGAGTTTTGGTGTT+GGG + chr4.4:1876303-1876322 MS.gene058551:CDS 45.0%
AATGGCCAGGATTTCCCCAA+TGG - chr4.4:1874013-1874032 None:intergenic 50.0%
ACAACGACTGGATCCACCAA+AGG - chr4.4:1874839-1874858 None:intergenic 50.0%
ACATGATCTAGTCGAGGAGG+AGG - chr4.4:1876105-1876124 None:intergenic 50.0%
ACATTGAGGATCCTCCACGT+CGG - chr4.4:1876231-1876250 None:intergenic 50.0%
ACGACCCCAACCTAACTTCT+TGG - chr4.4:1875347-1875366 None:intergenic 50.0%
AGGAGTGGATATGCAGCCTA+TGG + chr4.4:1875498-1875517 MS.gene058551:CDS 50.0%
AGTAAGCATGCCTTGTGGGA+GGG + chr4.4:1876128-1876147 MS.gene058551:CDS 50.0%
AGTGAGTGCCCTAACCTAGA+AGG - chr4.4:1875910-1875929 None:intergenic 50.0%
ATAGGGATCCTAGAGCTCAG+AGG + chr4.4:1874807-1874826 MS.gene058551:CDS 50.0%
ATGAAGCCCTTGGTCGTAAC+TGG + chr4.4:1873910-1873929 MS.gene058551:CDS 50.0%
ATTGTCACGCTTGACACCGA+GGG + chr4.4:1875198-1875217 MS.gene058551:CDS 50.0%
CACCAATGGCACCAAAGCTA+TGG + chr4.4:1874101-1874120 MS.gene058551:intron 50.0%
CATAGCACTTGTGTGGTTGG+AGG + chr4.4:1876282-1876301 MS.gene058551:CDS 50.0%
CATGAGCTGTGCCAAGATCT+CGG - chr4.4:1875754-1875773 None:intergenic 50.0%
CCAATCGATGCAACATCCCT+CGG - chr4.4:1875217-1875236 None:intergenic 50.0%
CCGAAGACTTCCTTCCATGT+CGG - chr4.4:1875715-1875734 None:intergenic 50.0%
CCGACATGGAAGGAAGTCTT+CGG + chr4.4:1875712-1875731 MS.gene058551:CDS 50.0%
CGGTGAATGAAGACCTGCAA+TGG - chr4.4:1876172-1876191 None:intergenic 50.0%
CTAATCTCCGACACAACGAC+TGG - chr4.4:1874851-1874870 None:intergenic 50.0%
CTACTCTTGAGGCTGTTTGC+TGG + chr4.4:1875383-1875402 MS.gene058551:CDS 50.0%
CTTCATGGACTCCTCCTTCT+AGG + chr4.4:1875893-1875912 MS.gene058551:CDS 50.0%
GAACGAGCACGTTAGGAAGA+TGG + chr4.4:1875270-1875289 MS.gene058551:CDS 50.0%
GAAGGAGGAGTCCATGAAGT+TGG - chr4.4:1875892-1875911 None:intergenic 50.0%
GATGCAAGCTATGGCACCAA+TGG + chr4.4:1874087-1874106 MS.gene058551:intron 50.0%
GATTTCCCCAATGGCACCAA+TGG - chr4.4:1874004-1874023 None:intergenic 50.0%
GCAAATTTAGGTGGTTCCCC+CGG - chr4.4:1876255-1876274 None:intergenic 50.0%
GCAACATGATCTAGTCGAGG+AGG - chr4.4:1876108-1876127 None:intergenic 50.0%
GCACTGAAATTGCAGCGTGA+AGG - chr4.4:1875439-1875458 None:intergenic 50.0%
GGACTCCTCCTTCTAGGTTA+GGG + chr4.4:1875899-1875918 MS.gene058551:CDS 50.0%
GGGCACTCACTCTCAGTTTA+TGG + chr4.4:1875919-1875938 MS.gene058551:CDS 50.0%
GGTCATAGCACTTGTGTGGT+TGG + chr4.4:1876279-1876298 MS.gene058551:CDS 50.0%
GTGCTTGTGCTGCAGGAAAT+AGG + chr4.4:1875455-1875474 MS.gene058551:CDS 50.0%
GTGGAGGATCCTCAATGTTC+CGG + chr4.4:1876233-1876252 MS.gene058551:CDS 50.0%
GTGTGTGATGTGTGTGTGTG+TGG + chr4.4:1874060-1874079 MS.gene058551:intron 50.0%
GTTGCAGTAAGCATGCCTTG+TGG + chr4.4:1876123-1876142 MS.gene058551:CDS 50.0%
TATCGTGCTCATGAAGCCCT+TGG + chr4.4:1873900-1873919 MS.gene058551:CDS 50.0%
TATTGTCACGCTTGACACCG+AGG + chr4.4:1875197-1875216 MS.gene058551:CDS 50.0%
TCGGGAGCTTACTACTCTTG+AGG + chr4.4:1875372-1875391 MS.gene058551:CDS 50.0%
TGGACTCCTCCTTCTAGGTT+AGG + chr4.4:1875898-1875917 MS.gene058551:CDS 50.0%
TGGAGGATCCTCAATGTTCC+GGG + chr4.4:1876234-1876253 MS.gene058551:CDS 50.0%
TGTGATGTGTGTGTGTGTGG+TGG + chr4.4:1874063-1874082 MS.gene058551:intron 50.0%
TTGGGGTCATAGCACTTGTG+TGG + chr4.4:1876275-1876294 MS.gene058551:CDS 50.0%
! GCAGCCGGTTCTTGCAATAT+AGG + chr4.4:1874789-1874808 MS.gene058551:intron 50.0%
! GGTTGGAGGGACTAGAGTTT+TGG + chr4.4:1876296-1876315 MS.gene058551:CDS 50.0%
! TGTGGTGGAGATGCAAGCTA+TGG + chr4.4:1874078-1874097 MS.gene058551:intron 50.0%
! TTTGGTGGATGTGGTAGGCA+AGG + chr4.4:1875646-1875665 MS.gene058551:CDS 50.0%
!! CGATTGGTTCTGTTTGCAGG+AGG + chr4.4:1875230-1875249 MS.gene058551:CDS 50.0%
!! GCACTTTGGTGCCATAGCTT+TGG - chr4.4:1874115-1874134 None:intergenic 50.0%
!! TGCCATAGCTTTGGTGCCAT+TGG - chr4.4:1874106-1874125 None:intergenic 50.0%
!! TTGGTGCCATTGGTGCCATT+GGG + chr4.4:1873995-1874014 MS.gene058551:intron 50.0%
!! TTGGTGTTGGGTGGACATAC+CGG + chr4.4:1876315-1876334 MS.gene058551:CDS 50.0%
!! TTTGGTGCCATTGGTGCCAT+TGG + chr4.4:1873994-1874013 MS.gene058551:intron 50.0%
ATTCAGCACCCATTCCTCCC+CGG - chr4.4:1876337-1876356 None:intergenic 55.0%
CAGTAAGCATGCCTTGTGGG+AGG + chr4.4:1876127-1876146 MS.gene058551:CDS 55.0%
CTGTGCCAAGATCTCGGAAG+AGG - chr4.4:1875748-1875767 None:intergenic 55.0%
GAGGATCCTCAATGTTCCGG+GGG + chr4.4:1876236-1876255 MS.gene058551:CDS 55.0%
GAGTGCCCTAACCTAGAAGG+AGG - chr4.4:1875907-1875926 None:intergenic 55.0%
GCATGGGGCAAAGAAGTGAC+AGG + chr4.4:1875304-1875323 MS.gene058551:CDS 55.0%
GGAGGATCCTCAATGTTCCG+GGG + chr4.4:1876235-1876254 MS.gene058551:CDS 55.0%
GGTGGATCCAGTCGTTGTGT+CGG + chr4.4:1874841-1874860 MS.gene058551:CDS 55.0%
TGCCAAGATCTCGGAAGAGG+CGG - chr4.4:1875745-1875764 None:intergenic 55.0%
TGCTCAACAGCCGACATGGA+AGG + chr4.4:1875702-1875721 MS.gene058551:CDS 55.0%
TGGACATACCGGGGAGGAAT+GGG + chr4.4:1876326-1876345 MS.gene058551:CDS 55.0%
TGGATGCTCAACAGCCGACA+TGG + chr4.4:1875698-1875717 MS.gene058551:CDS 55.0%
! AAGAGAGTGTGTGGCCCCAT+CGG - chr4.4:1875600-1875619 None:intergenic 55.0%
! CCGAGGGATGTTGCATCGAT+TGG + chr4.4:1875214-1875233 MS.gene058551:CDS 55.0%
! TAGGTTGGGGTCGTCTGACT+CGG + chr4.4:1875353-1875372 MS.gene058551:CDS 55.0%
! TGGTGTTGGGTGGACATACC+GGG + chr4.4:1876316-1876335 MS.gene058551:CDS 55.0%
!! ACGTCGGTTTCTCTTCGGCA+GGG - chr4.4:1876215-1876234 None:intergenic 55.0%
!! CCTCCACGTCGGTTTCTCTT+CGG - chr4.4:1876220-1876239 None:intergenic 55.0%
!! TGGTGCCATTGGGGAAATCC+TGG + chr4.4:1874005-1874024 MS.gene058551:intron 55.0%
!! TGGTGCCATTGGTGCCATTG+GGG + chr4.4:1873996-1874015 MS.gene058551:intron 55.0%
AGCTCAGAGGCGGCATCCTT+TGG + chr4.4:1874820-1874839 MS.gene058551:CDS 60.0%
AGGTTGGGGTCGTCTGACTC+GGG + chr4.4:1875354-1875373 MS.gene058551:CDS 60.0%
AGTGTGATATCCTCCCCGCC+CGG + chr4.4:1875574-1875593 MS.gene058551:CDS 60.0%
CCGAAGAGAAACCGACGTGG+AGG + chr4.4:1876217-1876236 MS.gene058551:CDS 60.0%
CTGCCGAAGAGAAACCGACG+TGG + chr4.4:1876214-1876233 MS.gene058551:CDS 60.0%
GGGATCCTAGAGCTCAGAGG+CGG + chr4.4:1874810-1874829 MS.gene058551:CDS 60.0%
GTGGACATACCGGGGAGGAA+TGG + chr4.4:1876325-1876344 MS.gene058551:CDS 60.0%
GTGGTTCCCCCGGAACATTG+AGG - chr4.4:1876245-1876264 None:intergenic 60.0%
TCAGAGGCGGCATCCTTTGG+TGG + chr4.4:1874823-1874842 MS.gene058551:CDS 60.0%
! GGTGTTGGGTGGACATACCG+GGG + chr4.4:1876317-1876336 MS.gene058551:CDS 60.0%
!! CACGTCGGTTTCTCTTCGGC+AGG - chr4.4:1876216-1876235 None:intergenic 60.0%
CACATCTCTTCGCGCTGCCG+TGG - chr4.4:1875136-1875155 None:intergenic 65.0%
CGCCGCCTCTTCCGAGATCT+TGG + chr4.4:1875740-1875759 MS.gene058551:CDS 65.0%
CGGCAGCGCGAAGAGATGTG+TGG + chr4.4:1875136-1875155 MS.gene058551:CDS 65.0%
GGATGCCGCCTCTGAGCTCT+AGG - chr4.4:1874818-1874837 None:intergenic 65.0%
GTTGGGTGGACATACCGGGG+AGG + chr4.4:1876320-1876339 MS.gene058551:CDS 65.0%
AGTGTGTGGCCCCATCGGCC+GGG - chr4.4:1875595-1875614 None:intergenic 70.0%
GAGTGTGTGGCCCCATCGGC+CGG - chr4.4:1875596-1875615 None:intergenic 70.0%
TCGGGCGGCTGCACAGATGC+CGG + chr4.4:1875802-1875821 MS.gene058551:CDS 70.0%
ATCCTCCCCGCCCGGCCGAT+GGG + chr4.4:1875582-1875601 MS.gene058551:CDS 75.0%
TATCCTCCCCGCCCGGCCGA+TGG + chr4.4:1875581-1875600 MS.gene058551:CDS 75.0%
! GTGTGGCCCCATCGGCCGGG+CGG - chr4.4:1875592-1875611 None:intergenic 80.0%
! TCCTCCCCGCCCGGCCGATG+GGG + chr4.4:1875583-1875602 MS.gene058551:CDS 80.0%
! TGTGGCCCCATCGGCCGGGC+GGG - chr4.4:1875591-1875610 None:intergenic 80.0%
! GTGGCCCCATCGGCCGGGCG+GGG - chr4.4:1875590-1875609 None:intergenic 85.0%
! GCCCCATCGGCCGGGCGGGG+AGG - chr4.4:1875587-1875606 None:intergenic 90.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 1873636 1876404 1873636 ID=MS.gene058551
chr4.4 mRNA 1873636 1876404 1873636 ID=MS.gene058551.t1;Parent=MS.gene058551
chr4.4 exon 1873636 1873931 1873636 ID=MS.gene058551.t1.exon1;Parent=MS.gene058551.t1
chr4.4 CDS 1873636 1873931 1873636 ID=cds.MS.gene058551.t1;Parent=MS.gene058551.t1
chr4.4 exon 1874793 1876404 1874793 ID=MS.gene058551.t1.exon2;Parent=MS.gene058551.t1
chr4.4 CDS 1874793 1876404 1874793 ID=cds.MS.gene058551.t1;Parent=MS.gene058551.t1
Gene Sequence

>MS.gene058551

ATGGCTATGGCAAGAGATAAAAACGAAGATGAAGAAGAGGTTCATAATCAAAATCAAAAGGGTGGGAAGAGATTGAAATGCATGAACATGAAGAAGAATGAACAAGAAGAAAAGCAAGTAGTAGATGAAGAAGAAAGTGAGCTTCCATTAAAACCAGAACTTTTCTTCTACCCAACAACACCAACATCTTTTGTTGTATCAGATGCACTTGAATCAGATTTTCCAATCATTTATGTCAACAAAGTCTTTGAAATCTCAACTGGGTATCGTGCTCATGAAGCCCTTGGTCGTAACTGGTAAGATTTTTTTTCATTTCTTGCATCATAATTTTGTTGGGGAAATACTTCCATGACATAGTTTTGGTGCCATTGGTGCCATTGGGGAAATCCTGGCCATTAATGTGTGTGGATGTGTGTGGGGATGTGTGTGTGATGTGTGTGTGTGTGGTGGAGATGCAAGCTATGGCACCAATGGCACCAAAGCTATGGCACCAAAGTGCTACTCATTTTGTTGGGGTTTATCTTTTTTATTGATTTAAGGTTGGAATTTATATAAAGTTTATAAATTTACTTAATTTAATTATGACCCACTTTCCTTAAACTAAAAATAAACCCTTTTATCAAACGGTGAAAATGATTCCATTCCTTTCAGATCTATACTATGTTGAAATTTTATAGTGCCAATTTTGTTTCATTTTGCAAACCACATTTTTATAGAATCCATTCTTTCTTATTTTGATTCCCCTAGCTTCCTTCTTATTGGATAATAAATAAATGTTCTTTTTATAAAGTTTCCTATTTCTCAAATTTTATTTTTATGATTTCACAATTTGAAGTTTATTTTCCATTCTATTGGCTTAACATTTTGACAACATGGTTACTTGGTAAACATTGTGATGTGCTTAGTACTTTCCATAATAATAATTAAGGTTCATCAATTGATTGTGATTGTGAATTGACTACTAGTGAAAAGATTTTGTTTTGAAGATTTTGATCAAAGATGTGACTCTCATAGAATCAATGATAGGGAACTGTCCTTTTTGGTTATATTTGGTATTTTCATTCCTTTTTTACAAGATATTTCTTTTAGATATTTTGATTTGGTTCACTTTTGACAAAATATACCTCTAATTGCTTTAGAATTTCTTTTGTTTGCAGCCGGTTCTTGCAATATAGGGATCCTAGAGCTCAGAGGCGGCATCCTTTGGTGGATCCAGTCGTTGTGTCGGAGATTAGAAGATGTCTTGAAGAAGGTATTGAGTTCCAAGGGGAACTTCTGAATTTCAGAAAGGATGGCACTCCCTTAGTCAACAGACTTAGGCTTACGCCAATTCATGATGATGATGGAGTCGTGACACACATAATAGGTATCCAAATCTTTTCCGAAGCAAACATAGACTTGAACCGTGTTTCCTATCCAGTTTTCAGAGAGACTTGCATTCAAGATTTTGATAAAAATGCTAAATATTCTCCCAAAAGTGGAAAATTATTGTACACTCCACGGCAGCGCGAAGAGATGTGTGGCATTCTTCAGCTTTCTGATGAAGTCTTGGCGCACAACATATTGTCACGCTTGACACCGAGGGATGTTGCATCGATTGGTTCTGTTTGCAGGAGGATCCGTCAATTGACAAAGAACGAGCACGTTAGGAAGATGGTTTGTCAAAACGCATGGGGCAAAGAAGTGACAGGTACATTGGAACTGATGACCAAGAAGTTAGGTTGGGGTCGTCTGACTCGGGAGCTTACTACTCTTGAGGCTGTTTGCTGGAAAAAAGTTACAGTTGGAGGCGGGGTTGAGCCTTCACGCTGCAATTTCAGTGCTTGTGCTGCAGGAAATAGGCTTGTATTATTTGGAGGTGAAGGAGTGGATATGCAGCCTATGGATGATACTTTTGTTCTAAATCTTGATGCTAAGAATCCAGAATGGCAAAGGGTTAGTGTGATATCCTCCCCGCCCGGCCGATGGGGCCACACACTCTCTTGCTTAAACAGTTCTTGGCTAGTTGTTTTTGGTGGATGTGGTAGGCAAGGATTGCTTAATGATGTGTTTGTGCTTGATTTGGATGCTCAACAGCCGACATGGAAGGAAGTCTTCGGTGAAGCGCCGCCTCTTCCGAGATCTTGGCACAGCTCATGCACAATTGAAGGCTCTAAATTGGTTGTCTCGGGCGGCTGCACAGATGCCGGAGTACTTCTTAGTGACACATATTTACTTGACCTCACAATAGATAATCCTACATGGAGAGAAATTCCAACTTCATGGACTCCTCCTTCTAGGTTAGGGCACTCACTCTCAGTTTATGGTAGGACAAAGATTCTCATGTTTGGTGGACTTGCAAAGAGTGGACACTTAAGGTTAAGATCAGGTGAGGCTTATGCAATTGATTTAGAAGCTGAACAACCACAATGGAGGCAACTAGAATGTAGTGCATTCACAGGTTTATCAAATCAAAATGCTGTTGTACCTCCTCCTCGACTAGATCATGTTGCAGTAAGCATGCCTTGTGGGAGGGTTATTATATTTGGAGGTTCCATTGCAGGTCTTCATTCACCGTCTCAGCTATTTCTATTGGACCCTGCCGAAGAGAAACCGACGTGGAGGATCCTCAATGTTCCGGGGGAACCACCTAAATTTGCTTGGGGTCATAGCACTTGTGTGGTTGGAGGGACTAGAGTTTTGGTGTTGGGTGGACATACCGGGGAGGAATGGGTGCTGAATGAGTTGCATGAGTTGTGCTTGGCAAGTCGACAAGACTCTGACATGTGA
Protein sequence

>MS.gene058551.t1

MAMARDKNEDEEEVHNQNQKGGKRLKCMNMKKNEQEEKQVVDEEESELPLKPELFFYPTTPTSFVVSDALESDFPIIYVNKVFEISTGYRAHEALGRNCRFLQYRDPRAQRRHPLVDPVVVSEIRRCLEEGIEFQGELLNFRKDGTPLVNRLRLTPIHDDDGVVTHIIGIQIFSEANIDLNRVSYPVFRETCIQDFDKNAKYSPKSGKLLYTPRQREEMCGILQLSDEVLAHNILSRLTPRDVASIGSVCRRIRQLTKNEHVRKMVCQNAWGKEVTGTLELMTKKLGWGRLTRELTTLEAVCWKKVTVGGGVEPSRCNFSACAAGNRLVLFGGEGVDMQPMDDTFVLNLDAKNPEWQRVSVISSPPGRWGHTLSCLNSSWLVVFGGCGRQGLLNDVFVLDLDAQQPTWKEVFGEAPPLPRSWHSSCTIEGSKLVVSGGCTDAGVLLSDTYLLDLTIDNPTWREIPTSWTPPSRLGHSLSVYGRTKILMFGGLAKSGHLRLRSGEAYAIDLEAEQPQWRQLECSAFTGLSNQNAVVPPPRLDHVAVSMPCGRVIIFGGSIAGLHSPSQLFLLDPAEEKPTWRILNVPGEPPKFAWGHSTCVVGGTRVLVLGGHTGEEWVLNELHELCLASRQDSDM