AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23326


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23326.t1 MTR_4g071150 98.026 152 1 1 1 150 1 152 9.78e-101 285
MS.gene23326.t1 MTR_2g096610 87.333 150 17 1 1 150 1 148 4.41e-83 241
MS.gene23326.t1 MTR_4g064005 94.000 150 7 1 1 150 1 148 1.32e-82 239
MS.gene23326.t1 MTR_4g063410 94.000 150 7 1 1 150 1 148 2.70e-82 239
MS.gene23326.t1 MTR_2g096570 91.558 154 8 2 1 150 1 153 5.64e-82 238
MS.gene23326.t1 MTR_4g063280 92.424 132 9 1 20 150 14 145 2.84e-81 236
MS.gene23326.t1 MTR_4g064967 93.333 150 8 1 1 150 1 148 2.98e-80 234
MS.gene23326.t1 MTR_8g086640 96.212 132 5 0 1 132 1 132 4.27e-75 221
MS.gene23326.t1 MTR_7g113160 79.699 133 26 1 10 142 13 144 5.01e-71 210
MS.gene23326.t1 MTR_7g108320 75.000 128 26 3 4 130 6 128 7.58e-60 182
MS.gene23326.t1 MTR_7g114060 74.219 128 29 1 3 130 2 125 7.85e-60 182
MS.gene23326.t1 MTR_7g114040 74.219 128 29 1 3 130 2 125 7.85e-60 182
MS.gene23326.t1 MTR_7g093910 73.228 127 31 1 4 130 5 128 9.33e-60 181
MS.gene23326.t1 MTR_2g082510 70.543 129 38 0 11 139 3 131 3.21e-59 180
MS.gene23326.t1 MTR_2g082220 73.950 119 31 0 11 129 3 121 1.65e-57 176
MS.gene23326.t1 MTR_6g005150 62.992 127 33 3 24 149 26 139 3.16e-47 150
MS.gene23326.t1 MTR_4g063300 75.238 105 21 1 45 149 10 109 3.34e-46 146
MS.gene23326.t1 MTR_7g114050 68.644 118 22 3 17 130 10 116 2.19e-45 145
MS.gene23326.t1 MTR_1g011750 60.714 112 42 2 21 131 23 133 3.59e-36 121
MS.gene23326.t1 MTR_1g011850 60.714 112 42 2 21 131 23 133 3.59e-36 121
MS.gene23326.t1 MTR_1g114050 60.550 109 41 2 21 128 20 127 2.90e-35 119
MS.gene23326.t1 MTR_1g071040 62.500 104 37 2 26 128 28 130 1.54e-34 117
MS.gene23326.t1 MTR_1g069770 48.958 96 48 1 45 139 9 104 5.64e-25 92.4
MS.gene23326.t1 MTR_4g048490 44.762 105 29 2 24 128 26 101 1.70e-16 70.9
MS.gene23326.t1 MTR_7g010440 70.588 51 15 0 78 128 122 172 2.24e-16 74.3
MS.gene23326.t1 MTR_4g005910 74.419 43 11 0 87 129 4 46 3.21e-16 68.6
MS.gene23326.t1 MTR_7g027610 41.525 118 42 4 20 135 10 102 5.62e-15 67.0
MS.gene23326.t1 MTR_0653s0030 54.545 55 25 0 55 109 43 97 4.58e-12 60.5
MS.gene23326.t1 MTR_2g096590 70.000 40 12 0 110 149 12 51 5.34e-12 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23326.t1 AT5G02560 79.739 153 28 1 1 150 1 153 1.48e-77 227
MS.gene23326.t1 AT5G27670 76.316 152 32 3 1 150 1 150 3.32e-74 218
MS.gene23326.t1 AT5G02560 68.927 177 28 2 1 150 1 177 1.06e-72 216
MS.gene23326.t1 AT5G59870 75.000 148 36 1 4 150 3 150 8.98e-72 212
MS.gene23326.t1 AT1G51060 76.667 120 28 0 11 130 3 122 9.15e-61 184
MS.gene23326.t1 AT4G27230 71.538 130 32 1 11 140 3 127 1.43e-59 181
MS.gene23326.t1 AT4G27230 71.538 130 32 1 11 140 3 127 1.43e-59 181
MS.gene23326.t1 AT3G20670 75.630 119 29 0 11 129 3 121 4.77e-59 179
MS.gene23326.t1 AT1G08880 71.654 127 33 1 7 130 2 128 3.47e-58 177
MS.gene23326.t1 AT5G54640 74.790 119 30 0 11 129 3 121 4.51e-58 177
MS.gene23326.t1 AT1G54690 72.868 129 29 3 3 130 5 128 1.19e-57 176
MS.gene23326.t1 AT2G38810 60.714 112 42 2 21 131 25 135 6.97e-37 123
MS.gene23326.t1 AT2G38810 60.714 112 42 2 21 131 25 135 6.97e-37 123
MS.gene23326.t1 AT2G38810 60.714 112 42 2 21 131 25 135 6.97e-37 123
MS.gene23326.t1 AT2G38810 60.714 112 42 2 21 131 25 135 6.97e-37 123
MS.gene23326.t1 AT1G52740 62.857 105 37 2 25 128 27 130 6.18e-35 118
MS.gene23326.t1 AT3G54560 60.185 108 41 2 25 131 29 135 1.25e-34 118
MS.gene23326.t1 AT3G54560 60.185 108 41 2 25 131 29 135 1.25e-34 118
MS.gene23326.t1 AT4G13570 48.913 92 45 2 32 122 28 118 1.97e-20 81.3
MS.gene23326.t1 AT4G13570 48.352 91 45 2 33 122 35 124 5.65e-20 80.1

Find 56 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGACTTTCCAGCCTTCTT+TGG 0.241789 4.4:+37457372 None:intergenic
AATCAGATCTAAGCCTTCTT+TGG 0.242120 4.4:+37457351 None:intergenic
GTCTTCTTAGGCAACAGAAT+TGG 0.279189 4.4:+37457423 None:intergenic
TTAGCCCTTTCAGTCTTCTT+AGG 0.279305 4.4:+37457411 None:intergenic
CGATTCTTCCGACTGGGAAT+TGG 0.285182 4.4:+37457691 None:intergenic
CATTCCAAGGCATTTGTTGC+TGG 0.294047 4.4:-37457526 MS.gene23326:CDS
CTTGTCACCGACTTCTTCCT+TGG 0.303917 4.4:+37457732 None:intergenic
GGCTCCCTTCTTTGTTGTCT+TGG 0.304409 4.4:+37457776 None:intergenic
GAGCATATCGACCTTTCTTC+AGG 0.357439 4.4:+37457661 None:intergenic
TGCTGCTGAGGTTCTTGAAT+TGG 0.361111 4.4:-37457586 MS.gene23326:CDS
GAGCTTGGGAAGTTGCTTGC+TGG 0.378028 4.4:-37457486 MS.gene23326:CDS
GGCATTTGTTGCTGGCTGTT+AGG 0.378218 4.4:-37457518 MS.gene23326:CDS
TCTTCTTAGGCAACAGAATT+GGG 0.398360 4.4:+37457424 None:intergenic
GAAGGGAGCCGGTGGAAGAA+AGG 0.417242 4.4:-37457763 MS.gene23326:CDS
AACAGAATTGGGTTGATGTT+CGG 0.418504 4.4:+37457435 None:intergenic
AAGGGAGCCGGTGGAAGAAA+GGG 0.429527 4.4:-37457762 MS.gene23326:CDS
CAATTCCCAGTCGGAAGAAT+CGG 0.431861 4.4:-37457690 MS.gene23326:CDS
AGAACTGCTGCTAGGTAAAC+CGG 0.439504 4.4:+37457618 None:intergenic
ACCTCCTCCCTTTCTTCCAC+CGG 0.471747 4.4:+37457755 None:intergenic
GCGTGTCGGCACCGGTGCTC+CGG 0.486643 4.4:-37457637 MS.gene23326:CDS
GAATTGGAGACCGGCTCTGA+TGG 0.486869 4.4:+37457707 None:intergenic
TATGCTCAGCGTGTCGGCAC+CGG 0.489995 4.4:-37457645 MS.gene23326:CDS
TCTTGAATATCTTGCTGCTG+AGG 0.496391 4.4:-37457598 MS.gene23326:CDS
AAGCACCAAGACAACAAAGA+AGG 0.501816 4.4:-37457781 MS.gene23326:CDS
GTTAGGAATGATGAAGAGCT+TGG 0.506747 4.4:-37457501 MS.gene23326:CDS
AGCAAGGAACCAAAGAAGGC+TGG 0.510603 4.4:-37457381 MS.gene23326:CDS
GCTGAGGTTCTTGAATTGGC+AGG 0.519701 4.4:-37457582 MS.gene23326:CDS
CTCGTGATAACAAGAAGAAT+AGG 0.522113 4.4:-37457551 MS.gene23326:CDS
TTAGGAATGATGAAGAGCTT+GGG 0.524474 4.4:-37457500 MS.gene23326:CDS
GGCTGGAAAGTCTCCAAAGA+AGG 0.525629 4.4:-37457364 MS.gene23326:CDS
ACAGCCAGCAACAAATGCCT+TGG 0.526781 4.4:+37457522 None:intergenic
TGCTAGGTAAACCGGAGCAC+CGG 0.537567 4.4:+37457626 None:intergenic
ATCGGTCGATACCTGAAGAA+AGG 0.548682 4.4:-37457672 MS.gene23326:CDS
TATCGACCGATTCTTCCGAC+TGG 0.549955 4.4:+37457684 None:intergenic
TGGTAGCAAGGAACCAAAGA+AGG 0.550474 4.4:-37457385 MS.gene23326:CDS
TGTTGCCTAAGAAGACTGAA+AGG 0.556495 4.4:-37457416 MS.gene23326:CDS
AGCACCAAGACAACAAAGAA+GGG 0.558080 4.4:-37457780 MS.gene23326:CDS
GAAGAAAGGGAGGAGGTCCA+AGG 0.558674 4.4:-37457749 MS.gene23326:CDS
TCCGACTGGGAATTGGAGAC+CGG 0.563841 4.4:+37457698 None:intergenic
AAGAACACCACCATGAGCTA+TGG 0.573393 4.4:+37457458 None:intergenic
AAGACAACAAAGAAGGGAGC+CGG 0.578427 4.4:-37457774 MS.gene23326:CDS
GATATTCAAGAACTGCTGCT+AGG 0.583613 4.4:+37457610 None:intergenic
GCTGGTGTTACCATAGCTCA+TGG 0.586669 4.4:-37457468 MS.gene23326:CDS
GTGACAAGATCCATCAGAGC+CGG 0.589197 4.4:-37457717 MS.gene23326:CDS
GTTGCCTAAGAAGACTGAAA+GGG 0.589268 4.4:-37457415 MS.gene23326:CDS
GCCGGTGGAAGAAAGGGAGG+AGG 0.593863 4.4:-37457756 MS.gene23326:CDS
AAGGGCTAACACTGGTAGCA+AGG 0.594830 4.4:-37457397 MS.gene23326:CDS
ACAACAAAGAAGGGAGCCGG+TGG 0.595449 4.4:-37457771 MS.gene23326:CDS
GGAGCCGGTGGAAGAAAGGG+AGG 0.604037 4.4:-37457759 MS.gene23326:CDS
GGTCGATATGCTCAGCGTGT+CGG 0.611319 4.4:-37457651 MS.gene23326:CDS
ATCGACCGATTCTTCCGACT+GGG 0.613102 4.4:+37457685 None:intergenic
AGGAGGTCCAAGGAAGAAGT+CGG 0.620460 4.4:-37457739 MS.gene23326:CDS
GCCGGTCTCCAATTCCCAGT+CGG 0.630576 4.4:-37457699 MS.gene23326:CDS
AGAAGAATAGGATCATTCCA+AGG 0.652341 4.4:-37457539 MS.gene23326:CDS
AAGACTGAAAGGGCTAACAC+TGG 0.665786 4.4:-37457405 MS.gene23326:CDS
GGTGTTACCATAGCTCATGG+TGG 0.742529 4.4:-37457465 MS.gene23326:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAAGAATAGGATCATTCCA+AGG - chr4.4:37457609-37457628 MS.gene23326:CDS 35.0%
CTCGTGATAACAAGAAGAAT+AGG - chr4.4:37457597-37457616 MS.gene23326:CDS 35.0%
TCTTCTTAGGCAACAGAATT+GGG + chr4.4:37457727-37457746 None:intergenic 35.0%
TTAGGAATGATGAAGAGCTT+GGG - chr4.4:37457648-37457667 MS.gene23326:CDS 35.0%
!! AACAGAATTGGGTTGATGTT+CGG + chr4.4:37457716-37457735 None:intergenic 35.0%
AAGCACCAAGACAACAAAGA+AGG - chr4.4:37457367-37457386 MS.gene23326:CDS 40.0%
AGCACCAAGACAACAAAGAA+GGG - chr4.4:37457368-37457387 MS.gene23326:CDS 40.0%
GATATTCAAGAACTGCTGCT+AGG + chr4.4:37457541-37457560 None:intergenic 40.0%
GTCTTCTTAGGCAACAGAAT+TGG + chr4.4:37457728-37457747 None:intergenic 40.0%
GTTAGGAATGATGAAGAGCT+TGG - chr4.4:37457647-37457666 MS.gene23326:CDS 40.0%
GTTGCCTAAGAAGACTGAAA+GGG - chr4.4:37457733-37457752 MS.gene23326:CDS 40.0%
TCTTGAATATCTTGCTGCTG+AGG - chr4.4:37457550-37457569 MS.gene23326:CDS 40.0%
TGTTGCCTAAGAAGACTGAA+AGG - chr4.4:37457732-37457751 MS.gene23326:CDS 40.0%
TTAGCCCTTTCAGTCTTCTT+AGG + chr4.4:37457740-37457759 None:intergenic 40.0%
AAGAACACCACCATGAGCTA+TGG + chr4.4:37457693-37457712 None:intergenic 45.0%
AAGACAACAAAGAAGGGAGC+CGG - chr4.4:37457374-37457393 MS.gene23326:CDS 45.0%
AAGACTGAAAGGGCTAACAC+TGG - chr4.4:37457743-37457762 MS.gene23326:CDS 45.0%
AGAACTGCTGCTAGGTAAAC+CGG + chr4.4:37457533-37457552 None:intergenic 45.0%
ATCGGTCGATACCTGAAGAA+AGG - chr4.4:37457476-37457495 MS.gene23326:CDS 45.0%
CAATTCCCAGTCGGAAGAAT+CGG - chr4.4:37457458-37457477 MS.gene23326:CDS 45.0%
GAGCATATCGACCTTTCTTC+AGG + chr4.4:37457490-37457509 None:intergenic 45.0%
TGCTGCTGAGGTTCTTGAAT+TGG - chr4.4:37457562-37457581 MS.gene23326:CDS 45.0%
TGGTAGCAAGGAACCAAAGA+AGG - chr4.4:37457763-37457782 MS.gene23326:CDS 45.0%
! CATTCCAAGGCATTTGTTGC+TGG - chr4.4:37457622-37457641 MS.gene23326:CDS 45.0%
AAGGGCTAACACTGGTAGCA+AGG - chr4.4:37457751-37457770 MS.gene23326:CDS 50.0%
ACAGCCAGCAACAAATGCCT+TGG + chr4.4:37457629-37457648 None:intergenic 50.0%
AGCAAGGAACCAAAGAAGGC+TGG - chr4.4:37457767-37457786 MS.gene23326:CDS 50.0%
AGGAGGTCCAAGGAAGAAGT+CGG - chr4.4:37457409-37457428 MS.gene23326:CDS 50.0%
CGATTCTTCCGACTGGGAAT+TGG + chr4.4:37457460-37457479 None:intergenic 50.0%
GCTGAGGTTCTTGAATTGGC+AGG - chr4.4:37457566-37457585 MS.gene23326:CDS 50.0%
GGAGACTTTCCAGCCTTCTT+TGG + chr4.4:37457779-37457798 None:intergenic 50.0%
GGCTGGAAAGTCTCCAAAGA+AGG - chr4.4:37457784-37457803 MS.gene23326:CDS 50.0%
GTGACAAGATCCATCAGAGC+CGG - chr4.4:37457431-37457450 MS.gene23326:CDS 50.0%
! ATCGACCGATTCTTCCGACT+GGG + chr4.4:37457466-37457485 None:intergenic 50.0%
! GGCATTTGTTGCTGGCTGTT+AGG - chr4.4:37457630-37457649 MS.gene23326:CDS 50.0%
! GGCTCCCTTCTTTGTTGTCT+TGG + chr4.4:37457375-37457394 None:intergenic 50.0%
! GGTGTTACCATAGCTCATGG+TGG - chr4.4:37457683-37457702 MS.gene23326:CDS 50.0%
! TATCGACCGATTCTTCCGAC+TGG + chr4.4:37457467-37457486 None:intergenic 50.0%
!! CTTGTCACCGACTTCTTCCT+TGG + chr4.4:37457419-37457438 None:intergenic 50.0%
!! GCTGGTGTTACCATAGCTCA+TGG - chr4.4:37457680-37457699 MS.gene23326:CDS 50.0%
AAGGGAGCCGGTGGAAGAAA+GGG - chr4.4:37457386-37457405 MS.gene23326:CDS 55.0%
ACAACAAAGAAGGGAGCCGG+TGG - chr4.4:37457377-37457396 MS.gene23326:CDS 55.0%
ACCTCCTCCCTTTCTTCCAC+CGG + chr4.4:37457396-37457415 None:intergenic 55.0%
GAAGAAAGGGAGGAGGTCCA+AGG - chr4.4:37457399-37457418 MS.gene23326:CDS 55.0%
GAGCTTGGGAAGTTGCTTGC+TGG - chr4.4:37457662-37457681 MS.gene23326:CDS 55.0%
GGTCGATATGCTCAGCGTGT+CGG - chr4.4:37457497-37457516 MS.gene23326:CDS 55.0%
TCCGACTGGGAATTGGAGAC+CGG + chr4.4:37457453-37457472 None:intergenic 55.0%
TGCTAGGTAAACCGGAGCAC+CGG + chr4.4:37457525-37457544 None:intergenic 55.0%
! GAATTGGAGACCGGCTCTGA+TGG + chr4.4:37457444-37457463 None:intergenic 55.0%
GAAGGGAGCCGGTGGAAGAA+AGG - chr4.4:37457385-37457404 MS.gene23326:CDS 60.0%
GCCGGTCTCCAATTCCCAGT+CGG - chr4.4:37457449-37457468 MS.gene23326:CDS 60.0%
! TATGCTCAGCGTGTCGGCAC+CGG - chr4.4:37457503-37457522 MS.gene23326:CDS 60.0%
GCCGGTGGAAGAAAGGGAGG+AGG - chr4.4:37457392-37457411 MS.gene23326:CDS 65.0%
GGAGCCGGTGGAAGAAAGGG+AGG - chr4.4:37457389-37457408 MS.gene23326:CDS 65.0%
!! GCGTGTCGGCACCGGTGCTC+CGG - chr4.4:37457511-37457530 MS.gene23326:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 37457359 37457811 37457359 ID=MS.gene23326
chr4.4 mRNA 37457359 37457811 37457359 ID=MS.gene23326.t1;Parent=MS.gene23326
chr4.4 exon 37457359 37457811 37457359 ID=MS.gene23326.t1.exon1;Parent=MS.gene23326.t1
chr4.4 CDS 37457359 37457811 37457359 ID=cds.MS.gene23326.t1;Parent=MS.gene23326.t1
Gene Sequence

>MS.gene23326

ATGGACGCAAGCACCAAGACAACAAAGAAGGGAGCCGGTGGAAGAAAGGGAGGAGGTCCAAGGAAGAAGTCGGTGACAAGATCCATCAGAGCCGGTCTCCAATTCCCAGTCGGAAGAATCGGTCGATACCTGAAGAAAGGTCGATATGCTCAGCGTGTCGGCACCGGTGCTCCGGTTTACCTAGCAGCAGTTCTTGAATATCTTGCTGCTGAGGTTCTTGAATTGGCAGGAAACGCTGCTCGTGATAACAAGAAGAATAGGATCATTCCAAGGCATTTGTTGCTGGCTGTTAGGAATGATGAAGAGCTTGGGAAGTTGCTTGCTGGTGTTACCATAGCTCATGGTGGTGTTCTTCCGAACATCAACCCAATTCTGTTGCCTAAGAAGACTGAAAGGGCTAACACTGGTAGCAAGGAACCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MS.gene23326.t1

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHLLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTERANTGSKEPKKAGKSPKKA