AlfalfaGEDB | MS.gene054018

M. sativa cultivar XinJiangDaYe / MS.gene054018

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054018.t1	XP_003596984.2	95.1	123	5	1	1	122	1	123	3.40E-47	197.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054018.t1	G7ITZ1	95.1	123	5	1	1	122	1	123	2.4e-47	197.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051761	MS.gene054018	0.802626	5.13E-49	-1.69E-46
MS.gene051914	MS.gene054018	0.817804	2.73E-52	-1.69E-46
MS.gene054018	MS.gene054633	0.801553	8.53E-49	-1.69E-46
MS.gene054018	MS.gene054662	0.852812	3.61E-61	-1.69E-46
MS.gene054018	MS.gene05640	0.800626	1.32E-48	-1.69E-46
MS.gene054018	MS.gene057367	0.802234	6.18E-49	-1.69E-46
MS.gene054018	MS.gene060291	0.802625	5.13E-49	-1.69E-46
MS.gene054018	MS.gene06093	0.80865	2.79E-50	-1.69E-46
MS.gene054018	MS.gene063235	0.815971	7.03E-52	-1.69E-46
MS.gene054018	MS.gene063308	0.839142	1.89E-57	-1.69E-46
MS.gene054018	MS.gene066026	0.804159	2.47E-49	-1.69E-46
MS.gene054018	MS.gene066779	0.850411	1.73E-60	-1.69E-46
MS.gene054018	MS.gene068585	0.802173	6.36E-49	-1.69E-46
MS.gene054018	MS.gene070800	0.820499	6.64E-53	-1.69E-46
MS.gene054018	MS.gene071422	0.811369	7.25E-51	-1.69E-46
MS.gene054018	MS.gene073059	0.811323	7.42E-51	-1.69E-46
MS.gene054018	MS.gene073062	0.808882	2.49E-50	-1.69E-46
MS.gene054018	MS.gene23326	0.806361	8.54E-50	-1.69E-46
MS.gene054018	MS.gene26406	0.820737	5.86E-53	-1.69E-46
MS.gene054018	MS.gene26721	0.807743	4.36E-50	-1.69E-46
MS.gene054018	MS.gene35818	0.988971	3.71E-176	-1.69E-46
MS.gene054018	MS.gene35821	0.989493	2.34E-178	-1.69E-46
MS.gene054018	MS.gene38724	0.813071	3.08E-51	-1.69E-46
MS.gene054018	MS.gene39911	0.848079	7.70E-60	-1.69E-46
MS.gene054018	MS.gene43987	0.806488	8.03E-50	-1.69E-46
MS.gene054018	MS.gene48492	0.805356	1.39E-49	-1.69E-46
MS.gene054018	MS.gene49349	0.84839	6.32E-60	-1.69E-46
MS.gene054018	MS.gene50576	0.852538	4.32E-61	-1.69E-46
MS.gene054018	MS.gene51554	0.827426	1.57E-54	-1.69E-46
MS.gene054018	MS.gene52980	0.826325	2.89E-54	-1.69E-46
MS.gene054018	MS.gene55400	0.822648	2.12E-53	-1.69E-46
MS.gene054018	MS.gene55401	0.839695	1.36E-57	-1.69E-46
MS.gene054018	MS.gene57750	0.802896	4.51E-49	-1.69E-46
MS.gene054018	MS.gene58112	0.837039	6.58E-57	-1.69E-46
MS.gene054018	MS.gene60269	0.833025	6.76E-56	-1.69E-46
MS.gene054018	MS.gene61618	0.813762	2.17E-51	-1.69E-46
MS.gene054018	MS.gene61684	0.82101	5.07E-53	-1.69E-46
MS.gene054018	MS.gene70258	0.801784	7.65E-49	-1.69E-46
MS.gene054018	MS.gene70263	0.810883	9.24E-51	-1.69E-46
MS.gene054018	MS.gene70279	0.815052	1.13E-51	-1.69E-46
MS.gene054018	MS.gene75915	0.812673	3.77E-51	-1.69E-46
MS.gene054018	MS.gene75918	0.812202	4.77E-51	-1.69E-46
MS.gene054018	MS.gene76829	0.800055	1.72E-48	-1.69E-46
MS.gene054018	MS.gene93058	0.816336	5.83E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054018.t1	MTR_2g088320	96.748	123	3	1	1	122	1	123	8.38e-82	235

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054018.t1	AT5G54970	44.706	85	44	2	13	94	15	99	2.74e-13	62.0

Find 50 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGGTGGTGGTGGTGAGGC+TGG	0.218611	2.1:+13348370	None:intergenic
CGGAGGCGAGCTAGGGTTCC+TGG	0.289851	2.1:+13348460	None:intergenic
ACCATCGGCGGCTGCGGCGG+CGG	0.308022	2.1:+13348330	None:intergenic
GATCTTGGTCTTGAATCATT+TGG	0.367487	2.1:-13348193	MS.gene054018:CDS
GGTTTCCCTTCCTTCTTCTC+CGG	0.381260	2.1:-13348310	MS.gene054018:CDS
GCTGCTGACTCAGCAGATCT+TGG	0.404060	2.1:-13348208	MS.gene054018:CDS
AGGTCTTGTTCTCTGCTTCA+TGG	0.408985	2.1:+13348516	None:intergenic
TGGAAGTGGTTTGGTAAAGT+AGG	0.419142	2.1:+13348496	None:intergenic
GGTGGTGGTGGTGAGGCTGG+TGG	0.422166	2.1:+13348373	None:intergenic
TGATCGGAGGGAGGTGGTGG+TGG	0.422451	2.1:+13348361	None:intergenic
GTTTCCCTTCCTTCTTCTCC+GGG	0.422834	2.1:-13348309	MS.gene054018:CDS
TTGAGTCACGGAGGCGAGCT+AGG	0.435623	2.1:+13348452	None:intergenic
AGGGAAACCATCGGCGGCTG+CGG	0.436360	2.1:+13348324	None:intergenic
GCGGCTGCGGCGGCGGTTTG+AGG	0.436624	2.1:+13348337	None:intergenic
GGAGTTGAGACGGTGAGATC+TGG	0.440317	2.1:+13348420	None:intergenic
TCCTTCTTCTCCGGGATCTA+CGG	0.444986	2.1:-13348301	MS.gene054018:CDS
GAGCTAGGGTTCCTGGTTTG+AGG	0.446447	2.1:+13348467	None:intergenic
GGCTGATCGGAGGGAGGTGG+TGG	0.453951	2.1:+13348358	None:intergenic
ACCGTAGATCCCGGAGAAGA+AGG	0.460453	2.1:+13348300	None:intergenic
ACCGCCGCCGCAGCCGCCGA+TGG	0.468049	2.1:-13348331	MS.gene054018:CDS
TTCCTGGTTTGAGGAATTTG+TGG	0.469740	2.1:+13348476	None:intergenic
AGGAATTTGTGGAAGTGGTT+TGG	0.473781	2.1:+13348487	None:intergenic
GTTTGAGGAATTTGTGGAAG+TGG	0.474784	2.1:+13348482	None:intergenic
TCTGCTGAGTCAGCAGCAGC+AGG	0.484937	2.1:+13348214	None:intergenic
CTTGGTCTTGAATCATTTGG+TGG	0.505567	2.1:-13348190	MS.gene054018:CDS
GAAACCATCGGCGGCTGCGG+CGG	0.505671	2.1:+13348327	None:intergenic
TCAGCAGCAGCAGGACTACC+AGG	0.510522	2.1:+13348223	None:intergenic
TGAGGCTGATCGGAGGGAGG+TGG	0.511381	2.1:+13348355	None:intergenic
GTGGTGGTGAGGCTGGTGGA+CGG	0.514928	2.1:+13348377	None:intergenic
GGAGGGAGGTGGTGGTGGTG+AGG	0.526828	2.1:+13348366	None:intergenic
GCGACGAGTTTCTTCCGTTG+TGG	0.540155	2.1:+13348268	None:intergenic
GCGGTTTGAGGCTGATCGGA+GGG	0.544351	2.1:+13348349	None:intergenic
TGAGTCACGGAGGCGAGCTA+GGG	0.544597	2.1:+13348453	None:intergenic
TAGATCCCGGAGAAGAAGGA+AGG	0.559260	2.1:+13348304	None:intergenic
GGCGGCGGTTTGAGGCTGAT+CGG	0.567721	2.1:+13348345	None:intergenic
GGCGGTTTGAGGCTGATCGG+AGG	0.568671	2.1:+13348348	None:intergenic
TGGCGGTTATCTTTGAGTCA+CGG	0.583959	2.1:+13348440	None:intergenic
TCTGAGAGAGGGAGTTGAGA+CGG	0.588321	2.1:+13348410	None:intergenic
ACATCGAGGACCGTAGATCC+CGG	0.588493	2.1:+13348291	None:intergenic
GTTTGAGGCTGATCGGAGGG+AGG	0.605220	2.1:+13348352	None:intergenic
TTCCACAAATTCCTCAAACC+AGG	0.613859	2.1:-13348478	MS.gene054018:CDS
GAAGAAGGAAGGGAAACCAT+CGG	0.615545	2.1:+13348315	None:intergenic
TTCTTCCGTTGTGGACATCG+AGG	0.627074	2.1:+13348277	None:intergenic
AGATCCCGGAGAAGAAGGAA+GGG	0.635957	2.1:+13348305	None:intergenic
GTAGATTGAGATCTGAGAGA+GGG	0.642846	2.1:+13348399	None:intergenic
GGTAGATTGAGATCTGAGAG+AGG	0.644225	2.1:+13348398	None:intergenic
GTTGAGACGGTGAGATCTGG+CGG	0.649621	2.1:+13348423	None:intergenic
ACGGTCCTCGATGTCCACAA+CGG	0.679494	2.1:-13348282	MS.gene054018:CDS
CGGTTATCTTTGAGTCACGG+AGG	0.737996	2.1:+13348443	None:intergenic
GAAGGAAGGGAAACCATCGG+CGG	0.745154	2.1:+13348318	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGTCAGTTTTCTTTGTTCC+TGG	-	chr2.1:13348441-13348460	MS.gene054018:CDS	35.0%
!!	GATCTTGGTCTTGAATCATT+TGG	-	chr2.1:13348489-13348508	MS.gene054018:CDS	35.0%
	AGGAATTTGTGGAAGTGGTT+TGG	+	chr2.1:13348198-13348217	None:intergenic	40.0%
	GTAGATTGAGATCTGAGAGA+GGG	+	chr2.1:13348286-13348305	None:intergenic	40.0%
	GTTTGAGGAATTTGTGGAAG+TGG	+	chr2.1:13348203-13348222	None:intergenic	40.0%
	TTCCACAAATTCCTCAAACC+AGG	-	chr2.1:13348204-13348223	MS.gene054018:CDS	40.0%
!	TTCCTGGTTTGAGGAATTTG+TGG	+	chr2.1:13348209-13348228	None:intergenic	40.0%
!!	CTTGGTCTTGAATCATTTGG+TGG	-	chr2.1:13348492-13348511	MS.gene054018:CDS	40.0%
!!	TGGAAGTGGTTTGGTAAAGT+AGG	+	chr2.1:13348189-13348208	None:intergenic	40.0%
	GAAGAAGGAAGGGAAACCAT+CGG	+	chr2.1:13348370-13348389	None:intergenic	45.0%
	GGTAGATTGAGATCTGAGAG+AGG	+	chr2.1:13348287-13348306	None:intergenic	45.0%
	TGGCGGTTATCTTTGAGTCA+CGG	+	chr2.1:13348245-13348264	None:intergenic	45.0%
	AGATCCCGGAGAAGAAGGAA+GGG	+	chr2.1:13348380-13348399	None:intergenic	50.0%
	CGGTTATCTTTGAGTCACGG+AGG	+	chr2.1:13348242-13348261	None:intergenic	50.0%
	GGTTTCCCTTCCTTCTTCTC+CGG	-	chr2.1:13348372-13348391	MS.gene054018:CDS	50.0%
	GTTTCCCTTCCTTCTTCTCC+GGG	-	chr2.1:13348373-13348392	MS.gene054018:CDS	50.0%
	TAGATCCCGGAGAAGAAGGA+AGG	+	chr2.1:13348381-13348400	None:intergenic	50.0%
	TCCTTCTTCTCCGGGATCTA+CGG	-	chr2.1:13348381-13348400	MS.gene054018:CDS	50.0%
	TCTGAGAGAGGGAGTTGAGA+CGG	+	chr2.1:13348275-13348294	None:intergenic	50.0%
	TTCTTCCGTTGTGGACATCG+AGG	+	chr2.1:13348408-13348427	None:intergenic	50.0%
	ACATCGAGGACCGTAGATCC+CGG	+	chr2.1:13348394-13348413	None:intergenic	55.0%
	ACCGTAGATCCCGGAGAAGA+AGG	+	chr2.1:13348385-13348404	None:intergenic	55.0%
	ACGGTCCTCGATGTCCACAA+CGG	-	chr2.1:13348400-13348419	MS.gene054018:CDS	55.0%
	GAAGGAAGGGAAACCATCGG+CGG	+	chr2.1:13348367-13348386	None:intergenic	55.0%
	GCGACGAGTTTCTTCCGTTG+TGG	+	chr2.1:13348417-13348436	None:intergenic	55.0%
	GGAGTTGAGACGGTGAGATC+TGG	+	chr2.1:13348265-13348284	None:intergenic	55.0%
	GTTGAGACGGTGAGATCTGG+CGG	+	chr2.1:13348262-13348281	None:intergenic	55.0%
!	GAGCTAGGGTTCCTGGTTTG+AGG	+	chr2.1:13348218-13348237	None:intergenic	55.0%
!!	GCTGCTGACTCAGCAGATCT+TGG	-	chr2.1:13348474-13348493	MS.gene054018:CDS	55.0%
	TCAGCAGCAGCAGGACTACC+AGG	+	chr2.1:13348462-13348481	None:intergenic	60.0%
	TCTGCTGAGTCAGCAGCAGC+AGG	+	chr2.1:13348471-13348490	None:intergenic	60.0%
	TGAGTCACGGAGGCGAGCTA+GGG	+	chr2.1:13348232-13348251	None:intergenic	60.0%
	TTGAGTCACGGAGGCGAGCT+AGG	+	chr2.1:13348233-13348252	None:intergenic	60.0%
!	GCGGTTTGAGGCTGATCGGA+GGG	+	chr2.1:13348336-13348355	None:intergenic	60.0%
!	GTTTGAGGCTGATCGGAGGG+AGG	+	chr2.1:13348333-13348352	None:intergenic	60.0%
	AGGGAAACCATCGGCGGCTG+CGG	+	chr2.1:13348361-13348380	None:intergenic	65.0%
	TGAGGCTGATCGGAGGGAGG+TGG	+	chr2.1:13348330-13348349	None:intergenic	65.0%
!	GGCGGCGGTTTGAGGCTGAT+CGG	+	chr2.1:13348340-13348359	None:intergenic	65.0%
!	GGCGGTTTGAGGCTGATCGG+AGG	+	chr2.1:13348337-13348356	None:intergenic	65.0%
!	GTGGTGGTGAGGCTGGTGGA+CGG	+	chr2.1:13348308-13348327	None:intergenic	65.0%
!	TGATCGGAGGGAGGTGGTGG+TGG	+	chr2.1:13348324-13348343	None:intergenic	65.0%
	CGGAGGCGAGCTAGGGTTCC+TGG	+	chr2.1:13348225-13348244	None:intergenic	70.0%
	GAAACCATCGGCGGCTGCGG+CGG	+	chr2.1:13348358-13348377	None:intergenic	70.0%
	GGCTGATCGGAGGGAGGTGG+TGG	+	chr2.1:13348327-13348346	None:intergenic	70.0%
!!	GGAGGGAGGTGGTGGTGGTG+AGG	+	chr2.1:13348319-13348338	None:intergenic	70.0%
!!	GGAGGTGGTGGTGGTGAGGC+TGG	+	chr2.1:13348315-13348334	None:intergenic	70.0%
!!	GGTGGTGGTGGTGAGGCTGG+TGG	+	chr2.1:13348312-13348331	None:intergenic	70.0%
!	ACCATCGGCGGCTGCGGCGG+CGG	+	chr2.1:13348355-13348374	None:intergenic	80.0%
!	GCGGCTGCGGCGGCGGTTTG+AGG	+	chr2.1:13348348-13348367	None:intergenic	80.0%
!	ACCGCCGCCGCAGCCGCCGA+TGG	-	chr2.1:13348351-13348370	MS.gene054018:CDS	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	13348168	13348536	13348168	ID=MS.gene054018
chr2.1	mRNA	13348168	13348536	13348168	ID=MS.gene054018.t1;Parent=MS.gene054018
chr2.1	exon	13348168	13348536	13348168	ID=MS.gene054018.t1.exon1;Parent=MS.gene054018.t1
chr2.1	CDS	13348168	13348536	13348168	ID=cds.MS.gene054018.t1;Parent=MS.gene054018.t1

Gene Sequence	>MS.gene054018 ATGAAGCAGAGAACAAGACCTACTTTACCAAACCACTTCCACAAATTCCTCAAACCAGGAACCCTAGCTCGCCTCCGTGACTCAAAGATAACCGCCAGATCTCACCGTCTCAACTCCCTCTCTCAGATCTCAATCTACCGTCCACCAGCCTCACCACCACCACCTCCCTCCGATCAGCCTCAAACCGCCGCCGCAGCCGCCGATGGTTTCCCTTCCTTCTTCTCCGGGATCTACGGTCCTCGATGTCCACAACGGAAGAAACTCGTCGCTGCAAAGTCAGTTTTCTTTGTTCCTGGTAGTCCTGCTGCTGCTGACTCAGCAGATCTTGGTCTTGAATCATTTGGTGGCGATATTATCGCTGCTAATTGA
Protein sequence	>MS.gene054018.t1 MKQRTRPTLPNHFHKFLKPGTLARLRDSKITARSHRLNSLSQISIYRPPASPPPPPSDQPQTAAAAADGFPSFFSGIYGPRCPQRKKLVAAKSVFFVPGSPAAADSADLGLESFGGDIIAAN