Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060291.t1 | XP_013470003.1 | 98.9 | 90 | 1 | 0 | 1 | 90 | 1 | 90 | 1.20E-38 | 168.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060291.t1 | Q948Z4 | 55.6 | 90 | 38 | 1 | 1 | 90 | 1 | 88 | 7.6e-21 | 100.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060291.t1 | A0A072VQU0 | 98.9 | 90 | 1 | 0 | 1 | 90 | 1 | 90 | 8.9e-39 | 168.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054018 | MS.gene060291 | 0.802625 | 5.13E-49 | -1.69E-46 |
| MS.gene054662 | MS.gene060291 | 0.843217 | 1.60E-58 | -1.69E-46 |
| MS.gene057367 | MS.gene060291 | 0.825154 | 5.47E-54 | -1.69E-46 |
| MS.gene060291 | MS.gene06093 | 0.817187 | 3.76E-52 | -1.69E-46 |
| MS.gene060291 | MS.gene063235 | 0.804276 | 2.34E-49 | -1.69E-46 |
| MS.gene060291 | MS.gene063308 | 0.806943 | 6.43E-50 | -1.69E-46 |
| MS.gene060291 | MS.gene066026 | 0.814331 | 1.63E-51 | -1.69E-46 |
| MS.gene060291 | MS.gene066779 | 0.816937 | 4.27E-52 | -1.69E-46 |
| MS.gene060291 | MS.gene071422 | 0.867088 | 1.77E-65 | -1.69E-46 |
| MS.gene060291 | MS.gene073059 | 0.858886 | 6.05E-63 | -1.69E-46 |
| MS.gene060291 | MS.gene073062 | 0.85309 | 3.01E-61 | -1.69E-46 |
| MS.gene060291 | MS.gene073063 | 0.806362 | 8.53E-50 | -1.69E-46 |
| MS.gene060291 | MS.gene22399 | 0.818991 | 1.47E-52 | -1.69E-46 |
| MS.gene060291 | MS.gene23326 | 0.812448 | 4.22E-51 | -1.69E-46 |
| MS.gene060291 | MS.gene23886 | 0.816739 | 4.73E-52 | -1.69E-46 |
| MS.gene060291 | MS.gene26406 | 0.809608 | 1.74E-50 | -1.69E-46 |
| MS.gene060291 | MS.gene29939 | 0.800154 | 1.65E-48 | -1.69E-46 |
| MS.gene060291 | MS.gene35821 | 0.804391 | 2.21E-49 | -1.69E-46 |
| MS.gene060291 | MS.gene36077 | 0.800181 | 1.63E-48 | -1.69E-46 |
| MS.gene060291 | MS.gene36778 | 0.802947 | 4.41E-49 | -1.69E-46 |
| MS.gene060291 | MS.gene37195 | 0.801738 | 7.81E-49 | -1.69E-46 |
| MS.gene060291 | MS.gene39911 | 0.85989 | 3.02E-63 | -1.69E-46 |
| MS.gene060291 | MS.gene42290 | 0.807564 | 4.75E-50 | -1.69E-46 |
| MS.gene060291 | MS.gene43981 | 0.805693 | 1.18E-49 | -1.69E-46 |
| MS.gene060291 | MS.gene47664 | 0.801257 | 9.80E-49 | -1.69E-46 |
| MS.gene060291 | MS.gene49349 | 0.873237 | 1.72E-67 | -1.69E-46 |
| MS.gene060291 | MS.gene50048 | 0.810254 | 1.26E-50 | -1.69E-46 |
| MS.gene060291 | MS.gene50051 | 0.810884 | 9.23E-51 | -1.69E-46 |
| MS.gene060291 | MS.gene50576 | 0.856636 | 2.81E-62 | -1.69E-46 |
| MS.gene060291 | MS.gene51554 | 0.847161 | 1.38E-59 | -1.69E-46 |
| MS.gene060291 | MS.gene52980 | 0.816382 | 5.69E-52 | -1.69E-46 |
| MS.gene060291 | MS.gene53188 | 0.840945 | 6.40E-58 | -1.69E-46 |
| MS.gene060291 | MS.gene55401 | 0.836678 | 8.13E-57 | -1.69E-46 |
| MS.gene060291 | MS.gene58112 | 0.830436 | 2.94E-55 | -1.69E-46 |
| MS.gene060291 | MS.gene60269 | 0.841051 | 6.01E-58 | -1.69E-46 |
| MS.gene060291 | MS.gene61618 | 0.809989 | 1.44E-50 | -1.69E-46 |
| MS.gene060291 | MS.gene61684 | 0.840235 | 9.82E-58 | -1.69E-46 |
| MS.gene060291 | MS.gene66043 | 0.82265 | 2.12E-53 | -1.69E-46 |
| MS.gene060291 | MS.gene66615 | 0.818128 | 2.30E-52 | -1.69E-46 |
| MS.gene060291 | MS.gene69198 | 0.811311 | 7.46E-51 | -1.69E-46 |
| MS.gene060291 | MS.gene69607 | 0.810624 | 1.05E-50 | -1.69E-46 |
| MS.gene060291 | MS.gene70491 | 0.823084 | 1.68E-53 | -1.69E-46 |
| MS.gene060291 | MS.gene71639 | 0.979368 | 8.14E-148 | -1.69E-46 |
| MS.gene060291 | MS.gene72164 | 0.82054 | 6.50E-53 | -1.69E-46 |
| MS.gene060291 | MS.gene75179 | 0.803214 | 3.88E-49 | -1.69E-46 |
| MS.gene060291 | MS.gene75761 | 0.830284 | 3.20E-55 | -1.69E-46 |
| MS.gene060291 | MS.gene75915 | 0.852999 | 3.19E-61 | -1.69E-46 |
| MS.gene060291 | MS.gene75918 | 0.862155 | 6.19E-64 | -1.69E-46 |
| MS.gene060291 | MS.gene76829 | 0.985224 | 6.63E-163 | -1.69E-46 |
| MS.gene060291 | MS.gene77200 | 0.800507 | 1.40E-48 | -1.69E-46 |
| MS.gene060291 | MS.gene78237 | 0.808326 | 3.27E-50 | -1.69E-46 |
| MS.gene060291 | MS.gene78325 | 0.803171 | 3.96E-49 | -1.69E-46 |
| MS.gene060291 | MS.gene91287 | 0.943547 | 9.89E-103 | -1.69E-46 |
| MS.gene060291 | MS.gene93058 | 0.818755 | 1.66E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060291.t1 | MTR_1g106915 | 98.889 | 90 | 1 | 0 | 1 | 90 | 1 | 90 | 2.56e-60 | 179 |
| MS.gene060291.t1 | MTR_1g018640 | 64.835 | 91 | 31 | 1 | 1 | 90 | 1 | 91 | 3.47e-30 | 102 |
| MS.gene060291.t1 | MTR_3g102660 | 67.692 | 65 | 21 | 0 | 26 | 90 | 21 | 85 | 5.08e-26 | 92.0 |
| MS.gene060291.t1 | MTR_5g036860 | 46.237 | 93 | 46 | 2 | 1 | 90 | 1 | 92 | 1.62e-20 | 78.6 |
| MS.gene060291.t1 | MTR_5g036640 | 47.561 | 82 | 41 | 1 | 9 | 90 | 4 | 83 | 1.77e-20 | 78.2 |
| MS.gene060291.t1 | MTR_5g036720 | 40.860 | 93 | 51 | 2 | 1 | 90 | 1 | 92 | 3.30e-17 | 70.1 |
| MS.gene060291.t1 | MTR_5g036880 | 41.935 | 93 | 50 | 2 | 1 | 90 | 1 | 92 | 6.62e-17 | 69.3 |
| MS.gene060291.t1 | MTR_5g095990 | 52.459 | 61 | 29 | 0 | 30 | 90 | 89 | 149 | 3.73e-16 | 68.9 |
| MS.gene060291.t1 | MTR_3g007900 | 52.459 | 61 | 29 | 0 | 30 | 90 | 94 | 154 | 4.10e-16 | 68.9 |
| MS.gene060291.t1 | MTR_1g096370 | 39.362 | 94 | 50 | 1 | 4 | 90 | 2 | 95 | 2.26e-15 | 65.5 |
| MS.gene060291.t1 | MTR_6g012360 | 34.951 | 103 | 48 | 2 | 7 | 90 | 5 | 107 | 7.55e-15 | 64.3 |
| MS.gene060291.t1 | MTR_1g025220 | 35.354 | 99 | 51 | 1 | 5 | 90 | 3 | 101 | 2.28e-14 | 63.2 |
| MS.gene060291.t1 | MTR_7g010580 | 46.774 | 62 | 33 | 0 | 29 | 90 | 48 | 109 | 2.56e-14 | 63.2 |
| MS.gene060291.t1 | MTR_3g037750 | 33.696 | 92 | 46 | 1 | 14 | 90 | 12 | 103 | 4.21e-14 | 62.4 |
| MS.gene060291.t1 | MTR_3g113890 | 32.653 | 98 | 51 | 1 | 8 | 90 | 6 | 103 | 1.86e-13 | 60.8 |
| MS.gene060291.t1 | MTR_7g090590 | 42.857 | 63 | 36 | 0 | 28 | 90 | 58 | 120 | 2.12e-13 | 60.8 |
| MS.gene060291.t1 | MTR_8g062800 | 45.902 | 61 | 32 | 1 | 31 | 90 | 52 | 112 | 2.18e-12 | 58.2 |
| MS.gene060291.t1 | MTR_1g025250 | 40.000 | 75 | 40 | 1 | 21 | 90 | 26 | 100 | 3.61e-12 | 57.4 |
| MS.gene060291.t1 | MTR_8g085470 | 45.679 | 81 | 41 | 1 | 13 | 90 | 15 | 95 | 7.18e-12 | 56.6 |
| MS.gene060291.t1 | MTR_6g007897 | 47.541 | 61 | 31 | 1 | 31 | 90 | 188 | 248 | 7.90e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060291.t1 | AT5G59845 | 60.440 | 91 | 33 | 2 | 1 | 90 | 1 | 89 | 3.81e-28 | 97.8 |
| MS.gene060291.t1 | AT2G39540 | 54.444 | 90 | 38 | 1 | 1 | 90 | 1 | 87 | 1.46e-26 | 93.6 |
| MS.gene060291.t1 | AT2G14900 | 69.492 | 59 | 18 | 0 | 32 | 90 | 50 | 108 | 4.29e-26 | 93.2 |
| MS.gene060291.t1 | AT1G10588 | 56.180 | 89 | 36 | 2 | 3 | 90 | 4 | 90 | 1.45e-25 | 91.3 |
| MS.gene060291.t1 | AT1G10588 | 53.933 | 89 | 37 | 2 | 3 | 90 | 4 | 89 | 3.37e-24 | 87.8 |
| MS.gene060291.t1 | AT1G74670 | 36.634 | 101 | 53 | 1 | 1 | 90 | 1 | 101 | 1.95e-16 | 68.2 |
| MS.gene060291.t1 | AT5G15230 | 30.189 | 106 | 58 | 1 | 1 | 90 | 1 | 106 | 2.51e-13 | 60.5 |
| MS.gene060291.t1 | AT4G09600 | 40.244 | 82 | 40 | 2 | 18 | 90 | 18 | 99 | 7.76e-13 | 58.9 |
| MS.gene060291.t1 | AT5G15230 | 44.262 | 61 | 34 | 0 | 30 | 90 | 23 | 83 | 4.46e-12 | 56.6 |
| MS.gene060291.t1 | AT1G22690 | 45.000 | 60 | 33 | 0 | 31 | 90 | 60 | 119 | 9.00e-11 | 54.3 |
| MS.gene060291.t1 | AT1G22690 | 45.000 | 60 | 33 | 0 | 31 | 90 | 60 | 119 | 9.00e-11 | 54.3 |
| MS.gene060291.t1 | AT1G22690 | 45.000 | 60 | 33 | 0 | 31 | 90 | 60 | 119 | 9.00e-11 | 54.3 |
Find 24 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTATGCTTCTCTTTCT+TGG | 0.113371 | 1.2:+79652296 | MS.gene060291:CDS |
| CAGAGACAAGGTTAACAATA+AGG | 0.287897 | 1.2:+79652594 | MS.gene060291:CDS |
| TGTGTTCCTTCTGGGACTTA+TGG | 0.316342 | 1.2:+79652547 | MS.gene060291:CDS |
| GTGTTCCTTCTGGGACTTAT+GGG | 0.319469 | 1.2:+79652548 | MS.gene060291:CDS |
| TAATTGACATCAAGGGCATT+TGG | 0.329972 | 1.2:-79652623 | None:intergenic |
| GAGTGCAAATGTGTTCCTTC+TGG | 0.347493 | 1.2:+79652538 | MS.gene060291:CDS |
| AATTGACATCAAGGGCATTT+GGG | 0.384143 | 1.2:-79652622 | None:intergenic |
| CGAACACTTGCTGCTACAAT+TGG | 0.398537 | 1.2:-79652452 | None:intergenic |
| CTAAAGCAGGGGTTCAAGAT+AGG | 0.424996 | 1.2:+79652485 | MS.gene060291:CDS |
| GGAGCTAGCCATTGCATATG+AGG | 0.425249 | 1.2:+79652317 | MS.gene060291:CDS |
| AGAGACAAGGTTAACAATAA+GGG | 0.465278 | 1.2:+79652595 | MS.gene060291:CDS |
| AGAAAGAGAAGCATAGAAGA+AGG | 0.472112 | 1.2:-79652293 | None:intergenic |
| AGTGCAAATGTGTTCCTTCT+GGG | 0.495711 | 1.2:+79652539 | MS.gene060291:CDS |
| GAAAGAGAAGCATAGAAGAA+GGG | 0.513355 | 1.2:-79652292 | None:intergenic |
| CAATTGTAGCAGCAAGTGTT+CGG | 0.519914 | 1.2:+79652453 | MS.gene060291:CDS |
| TCGGACAGATGCTCTAAAGC+AGG | 0.531159 | 1.2:+79652472 | MS.gene060291:CDS |
| CGGACAGATGCTCTAAAGCA+GGG | 0.574198 | 1.2:+79652473 | MS.gene060291:CDS |
| TTAACCTTGTCTCTGTAGCA+AGG | 0.582422 | 1.2:-79652586 | None:intergenic |
| ACATGAATCCTCATATGCAA+TGG | 0.604589 | 1.2:-79652325 | None:intergenic |
| AATAGCAACATGGTGAAGAG+AGG | 0.630987 | 1.2:-79652264 | None:intergenic |
| TTGTTCCCATAAGTCCCAGA+AGG | 0.664123 | 1.2:-79652553 | None:intergenic |
| ATGTCCTTGCTACAGAGACA+AGG | 0.668753 | 1.2:+79652582 | MS.gene060291:CDS |
| GGACAGATGCTCTAAAGCAG+GGG | 0.697192 | 1.2:+79652474 | MS.gene060291:CDS |
| AAGGGAACAGAATAGCAACA+TGG | 0.736288 | 1.2:-79652274 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTGTCTATTATATTCT+TGG | + | chr1.2:79652388-79652407 | MS.gene060291:intron | 15.0% |
| !!! | TGCATTTCCACTTTTAATAA+TGG | + | chr1.2:79652426-79652445 | MS.gene060291:intron | 25.0% |
| AGAGACAAGGTTAACAATAA+GGG | + | chr1.2:79652595-79652614 | MS.gene060291:CDS | 30.0% | |
| GAATCCTCTCATTTCATAAT+AGG | - | chr1.2:79652364-79652383 | None:intergenic | 30.0% | |
| TTCACCTATTATGAAATGAG+AGG | + | chr1.2:79652357-79652376 | MS.gene060291:intron | 30.0% | |
| ACATGAATCCTCATATGCAA+TGG | - | chr1.2:79652328-79652347 | None:intergenic | 35.0% | |
| CAGAGACAAGGTTAACAATA+AGG | + | chr1.2:79652594-79652613 | MS.gene060291:CDS | 35.0% | |
| TCTTCTATGCTTCTCTTTCT+TGG | + | chr1.2:79652296-79652315 | MS.gene060291:CDS | 35.0% | |
| TTGGCTGCCATTATTAAAAG+TGG | - | chr1.2:79652436-79652455 | None:intergenic | 35.0% | |
| ! | AGAAAGAGAAGCATAGAAGA+AGG | - | chr1.2:79652296-79652315 | None:intergenic | 35.0% |
| ! | GAAAGAGAAGCATAGAAGAA+GGG | - | chr1.2:79652295-79652314 | None:intergenic | 35.0% |
| AAGGGAACAGAATAGCAACA+TGG | - | chr1.2:79652277-79652296 | None:intergenic | 40.0% | |
| AATAGCAACATGGTGAAGAG+AGG | - | chr1.2:79652267-79652286 | None:intergenic | 40.0% | |
| AGTGCAAATGTGTTCCTTCT+GGG | + | chr1.2:79652539-79652558 | MS.gene060291:CDS | 40.0% | |
| CAATTGTAGCAGCAAGTGTT+CGG | + | chr1.2:79652453-79652472 | MS.gene060291:CDS | 40.0% | |
| TTAACCTTGTCTCTGTAGCA+AGG | - | chr1.2:79652589-79652608 | None:intergenic | 40.0% | |
| ATGTCCTTGCTACAGAGACA+AGG | + | chr1.2:79652582-79652601 | MS.gene060291:CDS | 45.0% | |
| CTAAAGCAGGGGTTCAAGAT+AGG | + | chr1.2:79652485-79652504 | MS.gene060291:CDS | 45.0% | |
| GAGTGCAAATGTGTTCCTTC+TGG | + | chr1.2:79652538-79652557 | MS.gene060291:CDS | 45.0% | |
| TTGTTCCCATAAGTCCCAGA+AGG | - | chr1.2:79652556-79652575 | None:intergenic | 45.0% | |
| ! | CGAACACTTGCTGCTACAAT+TGG | - | chr1.2:79652455-79652474 | None:intergenic | 45.0% |
| !! | GTGTTCCTTCTGGGACTTAT+GGG | + | chr1.2:79652548-79652567 | MS.gene060291:CDS | 45.0% |
| !! | TGTGTTCCTTCTGGGACTTA+TGG | + | chr1.2:79652547-79652566 | MS.gene060291:CDS | 45.0% |
| GGAGCTAGCCATTGCATATG+AGG | + | chr1.2:79652317-79652336 | MS.gene060291:CDS | 50.0% | |
| !! | CGGACAGATGCTCTAAAGCA+GGG | + | chr1.2:79652473-79652492 | MS.gene060291:CDS | 50.0% |
| !! | GGACAGATGCTCTAAAGCAG+GGG | + | chr1.2:79652474-79652493 | MS.gene060291:CDS | 50.0% |
| !! | TCGGACAGATGCTCTAAAGC+AGG | + | chr1.2:79652472-79652491 | MS.gene060291:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 79652258 | 79652636 | 79652258 | ID=MS.gene060291 |
| chr1.2 | mRNA | 79652258 | 79652636 | 79652258 | ID=MS.gene060291.t1;Parent=MS.gene060291 |
| chr1.2 | exon | 79652258 | 79652345 | 79652258 | ID=MS.gene060291.t1.exon1;Parent=MS.gene060291.t1 |
| chr1.2 | CDS | 79652258 | 79652345 | 79652258 | ID=cds.MS.gene060291.t1;Parent=MS.gene060291.t1 |
| chr1.2 | exon | 79652452 | 79652636 | 79652452 | ID=MS.gene060291.t1.exon2;Parent=MS.gene060291.t1 |
| chr1.2 | CDS | 79652452 | 79652636 | 79652452 | ID=cds.MS.gene060291.t1;Parent=MS.gene060291.t1 |
| Gene Sequence |
| Protein sequence |
