AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23562

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23562.t1 MTR_8g036160 95.000 360 15 1 1 360 1 357 0.0 614
MS.gene23562.t1 MTR_7g074935 69.972 363 77 10 1 360 1 334 9.02e-154 436
MS.gene23562.t1 MTR_4g101990 49.575 353 128 6 17 358 17 330 9.05e-78 242
MS.gene23562.t1 MTR_1g076710 42.779 367 148 11 1 351 1 321 7.90e-67 214
MS.gene23562.t1 MTR_1g061530 41.250 320 141 11 69 359 60 361 1.73e-57 191
MS.gene23562.t1 MTR_5g011680 41.319 288 121 6 15 272 4 273 2.96e-53 177
MS.gene23562.t1 MTR_5g010330 43.295 261 127 6 72 327 68 312 9.86e-53 177
MS.gene23562.t1 MTR_6g061670 51.142 219 87 8 64 272 43 251 8.08e-52 175
MS.gene23562.t1 MTR_6g061670 49.554 224 88 8 64 272 43 256 6.74e-51 172
MS.gene23562.t1 MTR_8g098390 47.761 201 101 3 73 273 96 292 7.30e-50 171
MS.gene23562.t1 MTR_8g031100 52.083 192 86 2 74 265 89 274 1.20e-48 167
MS.gene23562.t1 MTR_5g011620 42.586 263 120 6 15 273 4 239 1.58e-46 169
MS.gene23562.t1 MTR_7g077120 45.327 214 107 3 72 282 87 293 3.56e-43 153
MS.gene23562.t1 MTR_4g055803 41.629 221 120 4 55 272 40 254 2.77e-39 145
MS.gene23562.t1 MTR_4g098460 55.147 136 54 3 127 262 68 196 3.93e-38 138
MS.gene23562.t1 MTR_4g055833 40.000 220 123 4 56 272 38 251 5.08e-38 139
MS.gene23562.t1 MTR_4g098460 53.191 141 54 4 127 262 68 201 1.21e-35 131
MS.gene23562.t1 MTR_4g055813 51.145 131 59 3 147 276 21 147 2.21e-33 125
MS.gene23562.t1 MTR_5g011610 50.000 140 62 2 140 277 51 184 2.70e-33 126
MS.gene23562.t1 MTR_5g011610 49.533 107 50 1 134 240 201 303 8.55e-24 100
MS.gene23562.t1 MTR_1g079780 40.299 201 93 6 74 264 43 226 3.29e-33 126
MS.gene23562.t1 MTR_1g038960 38.983 236 124 8 71 303 82 300 2.47e-31 121
MS.gene23562.t1 MTR_7g116320 34.694 294 158 9 73 353 73 345 5.36e-31 121
MS.gene23562.t1 MTR_5g014360 39.720 214 105 7 72 278 118 314 3.62e-30 119
MS.gene23562.t1 MTR_4g055705 36.087 230 125 6 58 272 40 262 1.03e-27 110
MS.gene23562.t1 MTR_5g013390 37.129 202 110 8 71 267 78 267 4.66e-27 109
MS.gene23562.t1 MTR_7g116320 40.860 186 102 3 74 258 74 252 2.10e-26 106
MS.gene23562.t1 MTR_3g116100 36.667 240 121 11 71 306 90 302 5.09e-25 103
MS.gene23562.t1 MTR_6g060670 39.806 206 104 8 74 279 120 305 7.70e-25 103
MS.gene23562.t1 MTR_5g041430 37.173 191 103 7 73 260 49 225 3.11e-23 99.0
MS.gene23562.t1 MTR_1g038990 39.062 192 89 8 71 261 79 243 4.49e-23 98.6
MS.gene23562.t1 MTR_3g023560 43.651 126 64 3 143 265 60 181 2.03e-22 95.5
MS.gene23562.t1 MTR_3g068035 41.803 122 67 1 148 269 109 226 4.16e-20 90.1
MS.gene23562.t1 MTR_3g100470 39.837 123 71 3 147 267 103 224 2.52e-19 87.4
MS.gene23562.t1 MTR_5g066020 38.636 132 75 4 139 267 74 202 4.72e-19 85.9
MS.gene23562.t1 MTR_5g011700 46.729 107 52 3 156 261 10 112 9.99e-19 83.6
MS.gene23562.t1 MTR_4g098450 43.443 122 65 1 148 269 106 223 5.52e-18 83.6
MS.gene23562.t1 MTR_5g091630 34.158 202 84 5 62 262 34 187 2.46e-17 80.9
MS.gene23562.t1 MTR_7g080980 41.860 129 71 1 148 276 87 211 2.95e-17 81.3
MS.gene23562.t1 MTR_1g044155 41.803 122 67 1 148 269 156 273 1.18e-16 80.5
MS.gene23562.t1 MTR_5g011520 40.984 122 68 1 148 269 130 247 3.88e-16 78.6
MS.gene23562.t1 MTR_1g073860 40.164 122 69 1 148 269 124 241 4.15e-16 78.6
MS.gene23562.t1 MTR_8g036060 42.623 122 66 1 148 269 116 233 1.62e-15 76.6
MS.gene23562.t1 MTR_1g079810 39.344 122 70 1 148 269 104 221 1.25e-14 73.9
MS.gene23562.t1 MTR_5g080580 40.496 121 68 1 149 269 120 236 2.93e-14 73.2
MS.gene23562.t1 MTR_5g011590 35.514 107 66 2 96 202 36 139 3.67e-11 60.8
MS.gene23562.t1 MTR_5g011600 34.513 113 64 2 197 309 3 105 5.72e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23562.t1 AT4G12080 55.705 298 114 7 11 292 22 317 7.33e-91 276
MS.gene23562.t1 AT4G22770 55.036 278 104 7 6 274 13 278 5.17e-75 235
MS.gene23562.t1 AT4G22770 55.036 278 104 7 6 274 13 278 5.17e-75 235
MS.gene23562.t1 AT4G25320 58.049 205 74 5 73 267 85 287 1.07e-67 219
MS.gene23562.t1 AT4G00200 47.170 371 128 13 1 358 1 316 1.72e-67 215
MS.gene23562.t1 AT4G00200 47.170 371 128 13 1 358 1 316 1.72e-67 215
MS.gene23562.t1 AT2G33620 49.213 254 111 7 66 310 85 329 8.02e-64 207
MS.gene23562.t1 AT2G33620 49.213 254 111 7 66 310 85 329 8.02e-64 207
MS.gene23562.t1 AT2G33620 49.213 254 111 7 66 310 85 329 8.02e-64 207
MS.gene23562.t1 AT2G33620 49.213 254 111 7 66 310 85 329 8.02e-64 207
MS.gene23562.t1 AT5G62260 55.603 232 83 9 59 275 62 288 1.36e-63 208
MS.gene23562.t1 AT5G62260 55.603 232 83 9 59 275 62 288 1.36e-63 208
MS.gene23562.t1 AT5G62260 55.251 219 79 8 59 262 62 276 1.67e-60 196
MS.gene23562.t1 AT5G51590 52.273 220 73 4 73 262 77 294 4.43e-58 194
MS.gene23562.t1 AT2G45850 42.456 285 126 7 73 355 97 345 4.15e-52 176
MS.gene23562.t1 AT2G45850 42.456 285 126 7 73 355 97 345 4.15e-52 176
MS.gene23562.t1 AT1G63470 51.309 191 90 3 73 262 104 292 1.52e-50 173
MS.gene23562.t1 AT3G61310 51.415 212 87 6 70 277 97 296 2.30e-48 167
MS.gene23562.t1 AT2G45850 51.852 189 83 2 73 261 97 277 1.58e-47 162
MS.gene23562.t1 AT4G17950 42.308 234 85 7 73 271 130 348 7.44e-45 160
MS.gene23562.t1 AT1G63480 47.368 190 88 4 73 262 92 269 1.06e-42 152
MS.gene23562.t1 AT1G63480 47.368 190 88 4 73 262 92 269 1.06e-42 152
MS.gene23562.t1 AT1G63480 47.368 190 88 4 73 262 92 269 1.06e-42 152
MS.gene23562.t1 AT1G63480 47.368 190 88 4 73 262 92 269 1.06e-42 152
MS.gene23562.t1 AT1G63480 47.368 190 88 4 73 262 92 269 1.06e-42 152
MS.gene23562.t1 AT1G63480 47.368 190 88 4 73 262 98 275 1.15e-42 152
MS.gene23562.t1 AT1G63480 47.368 190 88 4 73 262 98 275 1.15e-42 152
MS.gene23562.t1 AT5G46640 44.978 229 97 8 64 275 87 303 2.48e-41 149
MS.gene23562.t1 AT3G04590 41.053 190 101 5 73 261 104 283 6.60e-35 130
MS.gene23562.t1 AT3G04590 41.053 190 101 5 73 261 104 283 9.48e-34 129
MS.gene23562.t1 AT2G36560 38.922 167 99 2 134 297 94 260 6.97e-27 112
MS.gene23562.t1 AT3G60870 40.171 117 64 2 145 261 86 196 1.19e-18 85.1
MS.gene23562.t1 AT5G49700 37.600 125 74 3 147 267 85 209 4.83e-18 83.6
MS.gene23562.t1 AT2G42940 41.129 124 69 1 139 262 74 193 2.06e-17 81.3
MS.gene23562.t1 AT3G55560 40.984 122 69 1 148 269 118 236 8.01e-17 80.5
MS.gene23562.t1 AT1G14490 32.418 182 93 5 147 319 51 211 1.02e-16 79.0
MS.gene23562.t1 AT1G14490 32.418 182 93 5 147 319 32 192 1.06e-16 78.2
MS.gene23562.t1 AT5G28590 38.333 120 49 2 142 261 27 121 6.27e-16 76.3
MS.gene23562.t1 AT2G35270 40.984 122 68 1 148 269 108 225 1.18e-15 77.0
MS.gene23562.t1 AT4G14465 41.228 114 63 1 148 261 97 206 1.27e-15 76.6
MS.gene23562.t1 AT4G17800 40.984 122 68 1 148 269 112 229 2.50e-15 75.9
MS.gene23562.t1 AT4G22810 41.600 125 66 2 148 269 135 255 6.17e-14 72.4
MS.gene23562.t1 AT4G12050 41.463 123 67 2 148 269 148 266 1.21e-13 71.6
MS.gene23562.t1 AT1G76500 33.333 123 69 2 148 261 102 220 2.25e-13 70.5
MS.gene23562.t1 AT3G04570 36.154 130 71 2 148 269 110 235 9.35e-13 68.6
MS.gene23562.t1 AT4G35390 41.379 116 62 2 148 261 93 204 2.05e-12 67.4
MS.gene23562.t1 AT2G45430 40.476 126 67 2 148 269 119 240 3.78e-12 67.0

Find 124 sgRNAs with CRISPR-Local

Find 290 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCGTCATCCAGATTCTTC+TGG 0.185964 8.4:-61255239 MS.gene23562:CDS
GCTGCAGCTGTGGCTACTTC+AGG 0.227842 8.4:+61257169 None:intergenic
CTTTCTCAGCACTATTAGTT+TGG 0.237596 8.4:+61253218 None:intergenic
TTTCTCAGCACTATTAGTTT+GGG 0.258422 8.4:+61253219 None:intergenic
AGAAGTGAAATTTGAGTTAA+GGG 0.292527 8.4:+61253150 None:intergenic
AGGGGTAGACCAAGGAAATA+TGG 0.299572 8.4:-61257049 MS.gene23562:CDS
TGATATGCGGATGGAGCTTC+TGG 0.301535 8.4:+61257232 None:intergenic
CCGCCTTTCGAAGGGAAAAC+TGG 0.306082 8.4:-61253117 MS.gene23562:CDS
CTGCCCTGCTTCTTGTTCCT+TGG 0.322269 8.4:+61254070 None:intergenic
GGACATGTACTGCAAAATTC+TGG 0.336452 8.4:-61253175 MS.gene23562:CDS
CTGTGGTTTCACTTGCTTCA+CGG 0.336722 8.4:+61256946 None:intergenic
CGATAATCAACTTAATTATC+AGG 0.338753 8.4:+61253034 None:intergenic
TTTGTAGGATATTACAAATA+AGG 0.345980 8.4:-61255336 MS.gene23562:intron
TTTGAGATACTTTCCTTGTC+TGG 0.346259 8.4:-61254117 MS.gene23562:CDS
GAGAAGTGAAATTTGAGTTA+AGG 0.349352 8.4:+61253149 None:intergenic
TCCGTCATCCAGATTCTTCT+GGG 0.353528 8.4:-61255238 MS.gene23562:CDS
CCATCCAAACCTACACTAAC+CGG 0.358354 8.4:+61257088 None:intergenic
GTAGTAGGCGGTGGAGTTGC+TGG 0.359916 8.4:-61254009 MS.gene23562:CDS
TGGTTTATTTCAGGTGGTGG+TGG 0.379225 8.4:-61253353 MS.gene23562:intron
TCAGGGTTATGAACCTGGTT+TGG 0.383570 8.4:+61257187 None:intergenic
TGGTTTGGAACTTCATTCCT+TGG 0.406728 8.4:+61257202 None:intergenic
TTCCCCGGTTAGTGTAGGTT+TGG 0.407247 8.4:-61257092 MS.gene23562:CDS
CTACTTCAGGGTTATGAACC+TGG 0.411417 8.4:+61257182 None:intergenic
CTGCAGCTGTGGCTACTTCA+GGG 0.413234 8.4:+61257170 None:intergenic
GTAGCCACAGCTGCAGCCGC+AGG 0.416742 8.4:-61257163 MS.gene23562:CDS
CCATCTGGCCCATATTTCCT+TGG 0.419968 8.4:+61257040 None:intergenic
CCGCCTACTACGCGACCATC+AGG 0.426116 8.4:+61254021 None:intergenic
AGGTATCAATCCTGCGGTTA+CGG 0.429267 8.4:-61257263 MS.gene23562:CDS
GTATCAGCAGCAGGAGCAGC+AGG 0.430433 8.4:+61257124 None:intergenic
ATATGTGTCTTATCGGCTAC+TGG 0.439406 8.4:-61255281 MS.gene23562:CDS
TCCTCCTGCGGCTGCAGCTG+TGG 0.444728 8.4:+61257159 None:intergenic
GCGAGCAATATGTGTCTTAT+CGG 0.444838 8.4:-61255288 MS.gene23562:CDS
CCGGTTAGTGTAGGTTTGGA+TGG 0.445659 8.4:-61257088 MS.gene23562:CDS
GCACTATTAGTTTGGGGTGG+CGG 0.448053 8.4:+61253226 None:intergenic
CTTCGAAAGGCGGTGGGTGG+AGG 0.455584 8.4:+61253127 None:intergenic
GAGGATGCCATGGGTGGCGG+TGG 0.459212 8.4:-61253196 MS.gene23562:CDS
GGTTTATTTCAGGTGGTGGT+GGG 0.467575 8.4:-61253352 MS.gene23562:intron
GGCGGAGCTGAAGATGAAAT+AGG 0.469098 8.4:+61256989 None:intergenic
CTCCACCCACCGCCTTTCGA+AGG 0.470896 8.4:-61253126 MS.gene23562:CDS
AACTAATAGTGCTGAGAAAG+AGG 0.473307 8.4:-61253215 MS.gene23562:CDS
GATTTACTGACGCACCTCAT+AGG 0.475961 8.4:+61255205 None:intergenic
ATGGAACCGTTAAGAAGAAA+AGG 0.482189 8.4:-61257069 MS.gene23562:CDS
TTCTCAGCACTATTAGTTTG+GGG 0.490838 8.4:+61253220 None:intergenic
GTCTCACTGGCAAGCCCTGA+TGG 0.493252 8.4:-61254036 MS.gene23562:CDS
TGATACTGTCGGTGTTTCCC+CGG 0.494440 8.4:-61257107 MS.gene23562:CDS
AGCCCTGATGGTCGCGTAGT+AGG 0.496107 8.4:-61254024 MS.gene23562:CDS
TATAGCTGCCACTCCTGTGC+AGG 0.498450 8.4:-61253980 MS.gene23562:intron
CTATTAGTTTGGGGTGGCGG+AGG 0.501818 8.4:+61253229 None:intergenic
GGCTGGTGCATAATCAGACT+TGG 0.502574 8.4:+61253285 None:intergenic
GCAAGTGAAACCACAGGGGA+TGG 0.503140 8.4:-61256939 MS.gene23562:CDS
CATTTCATCACTGTCAATGC+CGG 0.503879 8.4:-61255481 MS.gene23562:CDS
AAATTTGTGATGGATCATCT+AGG 0.506878 8.4:-61256899 MS.gene23562:intron
CGCCTTTCGAAGGGAAAACT+GGG 0.507842 8.4:-61253116 MS.gene23562:CDS
CCAAGGAAATATGGGCCAGA+TGG 0.510350 8.4:-61257040 MS.gene23562:CDS
GGGGTAGACCAAGGAAATAT+GGG 0.519638 8.4:-61257048 MS.gene23562:CDS
GGCATGTTGACAGACCCATC+TGG 0.529102 8.4:+61257025 None:intergenic
GAAAGGATGTTTACCTGCAC+AGG 0.532950 8.4:+61253967 None:intergenic
CAAGGAAATATGGGCCAGAT+GGG 0.533009 8.4:-61257039 MS.gene23562:CDS
GGTGTTTCCCCGGTTAGTGT+AGG 0.533689 8.4:-61257097 MS.gene23562:CDS
TCCCTTCGAAAGGCGGTGGG+TGG 0.534632 8.4:+61253124 None:intergenic
AGGTGGAATGAGTGTCTCAC+TGG 0.534737 8.4:-61254049 MS.gene23562:CDS
GTTCCTTGGTTAGTATCAGT+AGG 0.535654 8.4:+61254084 None:intergenic
TGGAACCGTTAAGAAGAAAA+GGG 0.536900 8.4:-61257068 MS.gene23562:CDS
GGCGCTATCACCGTAACCGC+AGG 0.538939 8.4:+61257253 None:intergenic
GATGAGAAGTCTTTAGAAGA+AGG 0.539192 8.4:+61256968 None:intergenic
CAAGGAACAAGAAGCAGGGC+AGG 0.539556 8.4:-61254069 MS.gene23562:CDS
GATGGTCGCGTAGTAGGCGG+TGG 0.544887 8.4:-61254018 MS.gene23562:CDS
GCCACAGCTGCAGCCGCAGG+AGG 0.547008 8.4:-61257160 MS.gene23562:CDS
CATCCAAACCTACACTAACC+GGG 0.547356 8.4:+61257089 None:intergenic
AAAGAGGATGCCATGGGTGG+CGG 0.548375 8.4:-61253199 MS.gene23562:CDS
TCCACCCACCGCCTTTCGAA+GGG 0.550500 8.4:-61253125 MS.gene23562:CDS
GGTCAACATGCACTCTATGC+CGG 0.563460 8.4:-61253095 MS.gene23562:CDS
CTAACCAAGGAACAAGAAGC+AGG 0.565981 8.4:-61254074 MS.gene23562:CDS
TGCACCAGCCACATTTACTC+AGG 0.575550 8.4:-61253272 MS.gene23562:CDS
AGGTGCTGCAGATACTGCAA+TGG 0.578343 8.4:+61253249 None:intergenic
TGCTGAGAAAGAGGATGCCA+TGG 0.579066 8.4:-61253206 MS.gene23562:CDS
GAAATAGGCAATGGTGACAA+TGG 0.584349 8.4:+61257004 None:intergenic
ACACCGACAGTATCAGCAGC+AGG 0.584936 8.4:+61257115 None:intergenic
GCTGGTGCATAATCAGACTT+GGG 0.586578 8.4:+61253286 None:intergenic
TACATGTCCACCGCCACCCA+TGG 0.588243 8.4:+61253189 None:intergenic
CGTGAAGCAAGTGAAACCAC+AGG 0.590436 8.4:-61256945 MS.gene23562:CDS
ATGGCCTGAGTAAATGTGGC+TGG 0.592651 8.4:+61253268 None:intergenic
TTCAGATCTTGATGGCCGCT+AGG 0.596191 8.4:+61253319 None:intergenic
ATATGCATGGAAGGAAGAGA+AGG 0.600541 8.4:-61257283 None:intergenic
GCTCCTGCTGCTGATACTGT+CGG 0.601485 8.4:-61257118 MS.gene23562:CDS
TGCATTCAAACATACCTCGC+CGG 0.603353 8.4:+61255462 None:intergenic
ACATTCAATATATGCATGGA+AGG 0.604410 8.4:-61257292 None:intergenic
CCTGATGGTCGCGTAGTAGG+CGG 0.607126 8.4:-61254021 MS.gene23562:CDS
GATGTTTACCTGCACAGGAG+TGG 0.611223 8.4:+61253972 None:intergenic
CTCTTGTATTCCATCCCCTG+TGG 0.611712 8.4:+61256929 None:intergenic
TCAGCACTATTAGTTTGGGG+TGG 0.613413 8.4:+61253223 None:intergenic
GCCATCAAGATCTGAAAACA+AGG 0.613990 8.4:-61253312 MS.gene23562:CDS
GGAACAAGAAGCAGGGCAGG+TGG 0.614997 8.4:-61254066 MS.gene23562:CDS
CCGTCATCCAGATTCTTCTG+GGG 0.615042 8.4:-61255237 MS.gene23562:CDS
CGTCATCCAGATTCTTCTGG+GGG 0.617667 8.4:-61255236 MS.gene23562:CDS
TGCAATGGCCTGAGTAAATG+TGG 0.618756 8.4:+61253264 None:intergenic
CACAGGGGATGGAATACAAG+AGG 0.618940 8.4:-61256928 MS.gene23562:CDS
GAAATGAATGGGCACTCTGA+TGG 0.621101 8.4:-61255520 MS.gene23562:CDS
CCCCAGAAGAATCTGGATGA+CGG 0.622155 8.4:+61255237 None:intergenic
GCTGAAGATGAAATAGGCAA+TGG 0.637503 8.4:+61256995 None:intergenic
CGCCTACTACGCGACCATCA+GGG 0.640739 8.4:+61254022 None:intergenic
GAGAAGTCTTTAGAAGAAGG+CGG 0.641757 8.4:+61256971 None:intergenic
AGAAGAAAAGGGGTAGACCA+AGG 0.648040 8.4:-61257057 MS.gene23562:CDS
AAGGAATGAAGTTCCAAACC+AGG 0.649098 8.4:-61257200 MS.gene23562:CDS
ATCCAAACCTACACTAACCG+GGG 0.651483 8.4:+61257090 None:intergenic
GGTGCGACATGATATGCGGA+TGG 0.659191 8.4:+61257223 None:intergenic
TAACCAAGGAACAAGAAGCA+GGG 0.661712 8.4:-61254073 MS.gene23562:CDS
GCATTGACAGTGATGAAATG+CGG 0.670620 8.4:+61255484 None:intergenic
GATAAGACACATATTGCTCG+CGG 0.677068 8.4:+61255290 None:intergenic
GTGAAGCAAGTGAAACCACA+GGG 0.677228 8.4:-61256944 MS.gene23562:CDS
CCGCATATCATGTCGCACCA+AGG 0.679104 8.4:-61257219 MS.gene23562:CDS
CATCACTGTCAATGCCGGCG+AGG 0.679492 8.4:-61255476 MS.gene23562:intron
GAGAAAGAGGATGCCATGGG+TGG 0.680486 8.4:-61253202 MS.gene23562:CDS
ACAGGGGATGGAATACAAGA+GGG 0.685377 8.4:-61256927 MS.gene23562:CDS
GTGGGAAGCTTTCTGCCTAG+CGG 0.688039 8.4:-61253334 MS.gene23562:CDS
GGAACCGTTAAGAAGAAAAG+GGG 0.688506 8.4:-61257067 MS.gene23562:CDS
TATGCCGGACACAAGAAAAG+CGG 0.691783 8.4:-61253080 MS.gene23562:CDS
ATGCCTACTGATACTAACCA+AGG 0.692561 8.4:-61254087 MS.gene23562:CDS
CCTTGGTGCGACATGATATG+CGG 0.694472 8.4:+61257219 None:intergenic
AGGCATGAATGATCCAGACA+AGG 0.701025 8.4:+61254104 None:intergenic
AGCAGCAGTAGATCCTCCTG+CGG 0.704949 8.4:+61257147 None:intergenic
AAGAGAAGGTATCAATCCTG+CGG 0.707222 8.4:-61257269 MS.gene23562:CDS
GCTGAGAAAGAGGATGCCAT+GGG 0.708603 8.4:-61253205 MS.gene23562:CDS
TGAAGCAAGTGAAACCACAG+GGG 0.784596 8.4:-61256943 MS.gene23562:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTATTCATTATAACTT+TGG + chr8.4:61254947-61254966 None:intergenic 10.0%
!!! AATTCAGAAATATTTTTTGT+TGG - chr8.4:61254766-61254785 MS.gene23562:intron 15.0%
!!! ATTTTGTAAATTAATCAAGA+TGG - chr8.4:61255405-61255424 MS.gene23562:intron 15.0%
!!! GATTGATAATATTTATAATC+AGG - chr8.4:61255267-61255286 MS.gene23562:CDS 15.0%
!! AATTAATTAATTCCGCTATT+GGG - chr8.4:61253961-61253980 MS.gene23562:intron 20.0%
!! ACTGAAATTAATATAAGCAA+AGG - chr8.4:61254363-61254382 MS.gene23562:intron 20.0%
!! ACTGAAATTAATATAAGCAA+CGG - chr8.4:61254363-61254382 MS.gene23562:intron 20.0%
!! ACTGAAATTAATATAAGCAA+CGG + chr8.4:61254382-61254363 None:intergenic 20.0%
!! AGCATTAAATCAATGTATAA+AGG + chr8.4:61253460-61253479 None:intergenic 20.0%
!! ATATTAAACAGAAGATATTG+TGG + chr8.4:61256153-61256172 None:intergenic 20.0%
!! ATTCTTTACAAATTACAATG+AGG + chr8.4:61254282-61254301 None:intergenic 20.0%
!! ATTTGTAAAGAATTACAATG+AGG - chr8.4:61254311-61254330 MS.gene23562:intron 20.0%
!! TAAGTAAATTCTATGTATCA+AGG - chr8.4:61254102-61254121 MS.gene23562:CDS 20.0%
!! TAAGTAAATTCTATGTATCA+AGG + chr8.4:61254121-61254102 None:intergenic 20.0%
!! TACACAACTTGTATTTATAA+CGG + chr8.4:61253840-61253859 None:intergenic 20.0%
!! TTCTTTACAAATTACAATGA+GGG + chr8.4:61254281-61254300 None:intergenic 20.0%
!! TTTGTAAAGAATTACAATGA+GGG - chr8.4:61254312-61254331 MS.gene23562:intron 20.0%
!! TTTGTAGGATATTACAAATA+AGG - chr8.4:61254986-61255005 MS.gene23562:intron 20.0%
!!! ATCGATATTTGATTATGTTT+TGG + chr8.4:61255945-61255964 None:intergenic 20.0%
!!! ATGAATTCAAAAACATCTTT+TGG - chr8.4:61253923-61253942 MS.gene23562:intron 20.0%
!!! CTTTTGTTTATAAACAGTAA+CGG - chr8.4:61254415-61254434 MS.gene23562:intron 20.0%
!!! GTTTTTTGTTTATATGAGAA+TGG - chr8.4:61255318-61255337 MS.gene23562:CDS 20.0%
!!! TACACAACTTGTTTTTATAA+TGG + chr8.4:61254473-61254492 None:intergenic 20.0%
!!! TATTTTTCTTGGTTTATTTC+AGG - chr8.4:61256960-61256979 MS.gene23562:CDS 20.0%
! AAACAGTGAAATATTTGGAA+AGG + chr8.4:61256586-61256605 None:intergenic 25.0%
! AAACATCAACAAAAGGAAAA+AGG + chr8.4:61253537-61253556 None:intergenic 25.0%
! AAGGCAACTTGTATTTATAA+TGG - chr8.4:61254121-61254140 MS.gene23562:CDS 25.0%
! AAGGCAACTTGTATTTATAA+TGG + chr8.4:61254140-61254121 None:intergenic 25.0%
! AATATAGTTGCCAAACAAAT+AGG - chr8.4:61256460-61256479 MS.gene23562:intron 25.0%
! AATGTCTTCTCATACTAAAT+AGG - chr8.4:61256692-61256711 MS.gene23562:intron 25.0%
! AATTCTCAATCCTATTTGTT+TGG + chr8.4:61256473-61256492 None:intergenic 25.0%
! ATACCTTAATAAGTGTGAAA+TGG + chr8.4:61254205-61254224 None:intergenic 25.0%
! ATAGGAAACAGTGAAATATT+TGG + chr8.4:61256591-61256610 None:intergenic 25.0%
! CTATGAAGTTGTACAATTAA+AGG + chr8.4:61253737-61253756 None:intergenic 25.0%
! CTGTTTACAAAGATTTGTAT+AGG + chr8.4:61256609-61256628 None:intergenic 25.0%
! GAATTAATTAATTCCGCTAT+TGG - chr8.4:61253960-61253979 MS.gene23562:intron 25.0%
! GTTCAGTAAATTAATCAAGA+TGG - chr8.4:61255359-61255378 MS.gene23562:intron 25.0%
! TTAAATTCAATCACATGTGT+TGG - chr8.4:61256867-61256886 MS.gene23562:intron 25.0%
! TTATGTAAAGTATGGATCTA+AGG - chr8.4:61255221-61255240 MS.gene23562:CDS 25.0%
! TTGTTTGGCAACTATATTAA+GGG + chr8.4:61256458-61256477 None:intergenic 25.0%
! TTTGTTTGGCAACTATATTA+AGG + chr8.4:61256459-61256478 None:intergenic 25.0%
!! AGAAGTGAAATTTGAGTTAA+GGG + chr8.4:61257175-61257194 None:intergenic 25.0%
!! AGGATCACTTATATTTTTCT+TGG - chr8.4:61256949-61256968 MS.gene23562:CDS 25.0%
!! CATTATAACTTTGGAAGTAA+TGG + chr8.4:61254938-61254957 None:intergenic 25.0%
!! GAATGTTGATGTATTGATAT+TGG - chr8.4:61256125-61256144 MS.gene23562:intron 25.0%
!! GTTCTGTTTTATGTAAAGTA+TGG - chr8.4:61255213-61255232 MS.gene23562:intron 25.0%
!! TATTATCTTGCTATACTCTT+TGG - chr8.4:61254963-61254982 MS.gene23562:intron 25.0%
!! TCAAAGAGGTTTTCAAAATA+GGG + chr8.4:61255634-61255653 None:intergenic 25.0%
!! TGATATCATTTTCACAACAA+GGG - chr8.4:61255009-61255028 MS.gene23562:intron 25.0%
!! TTAGGTATTGCAATAATCAT+CGG - chr8.4:61255245-61255264 MS.gene23562:CDS 25.0%
!! TTTTCAAAATAGGGTCAATT+TGG + chr8.4:61255625-61255644 None:intergenic 25.0%
!!! AAATTACAATGAGGGTATTA+TGG + chr8.4:61254273-61254292 None:intergenic 25.0%
!!! AAATTACAATGAGGGTATTT+TGG - chr8.4:61254528-61254547 MS.gene23562:intron 25.0%
!!! AATTACAATGAGGGTATTAT+GGG + chr8.4:61254272-61254291 None:intergenic 25.0%
!!! AATTACAATGAGGGTATTTT+GGG - chr8.4:61254529-61254548 MS.gene23562:intron 25.0%
!!! TATTTTGAAAACCTCTTTGA+GGG - chr8.4:61255634-61255653 MS.gene23562:intron 25.0%
!!! TTCCTCTGTTTTTTGTTTTT+TGG - chr8.4:61255804-61255823 MS.gene23562:intron 25.0%
AAATGACTTTCACAAAAGTC+TGG - chr8.4:61256026-61256045 MS.gene23562:intron 30.0%
AACATAATAGTAGTCAATCC+CGG + chr8.4:61255992-61256011 None:intergenic 30.0%
AACCTAGAAAGTTGTAAACT+CGG + chr8.4:61253778-61253797 None:intergenic 30.0%
AAGTTGTACAATTAAAGGTC+AGG + chr8.4:61253732-61253751 None:intergenic 30.0%
AATTTGTATAACGCTTAGGA+AGG + chr8.4:61254908-61254927 None:intergenic 30.0%
ACAATTAAAGGTCAGGAATA+TGG + chr8.4:61253725-61253744 None:intergenic 30.0%
ACACTTCAAACATCAACAAA+AGG + chr8.4:61253544-61253563 None:intergenic 30.0%
ACATTTCGCAAATTACAATG+AGG - chr8.4:61254519-61254538 MS.gene23562:intron 30.0%
ACCATAAAACAAAAGAAAGC+AGG + chr8.4:61255756-61255775 None:intergenic 30.0%
ACTATATTAAGGGAAAATGG+TGG + chr8.4:61256448-61256467 None:intergenic 30.0%
AGACATAGAATTGCAAGTTT+AGG + chr8.4:61256719-61256738 None:intergenic 30.0%
ATACCTTAACAAGTGTGAAA+TGG - chr8.4:61254596-61254615 MS.gene23562:intron 30.0%
ATATGTTCAGTAGTCAATAG+TGG - chr8.4:61255504-61255523 MS.gene23562:CDS 30.0%
CACCAAAAAACAAAAAACAG+AGG + chr8.4:61255809-61255828 None:intergenic 30.0%
CAGAAGCTTCAAATGAATTT+AGG + chr8.4:61255182-61255201 None:intergenic 30.0%
CATCAATTTGTATAACGCTT+AGG + chr8.4:61254912-61254931 None:intergenic 30.0%
CATTTCGCAAATTACAATGA+GGG - chr8.4:61254520-61254539 MS.gene23562:intron 30.0%
GACATAGAATTGCAAGTTTA+GGG + chr8.4:61256718-61256737 None:intergenic 30.0%
GCAACTATATTAAGGGAAAA+TGG + chr8.4:61256451-61256470 None:intergenic 30.0%
GTATAGGAATCATGTTAACT+TGG + chr8.4:61256779-61256798 None:intergenic 30.0%
TCACCATTTCACACTTATTA+AGG - chr8.4:61254199-61254218 MS.gene23562:intron 30.0%
TTCTACTAATGTATTGAGTC+AGG - chr8.4:61255452-61255471 MS.gene23562:intron 30.0%
TTGTTTATAAACAGTAACGG+AGG - chr8.4:61254418-61254437 MS.gene23562:intron 30.0%
! AAAGTATGGATCTAAGGTTT+AGG - chr8.4:61255227-61255246 MS.gene23562:CDS 30.0%
! AAATTTGAGAGCTTTTGTTG+TGG + chr8.4:61253653-61253672 None:intergenic 30.0%
! CTCAAAGAGGTTTTCAAAAT+AGG + chr8.4:61255635-61255654 None:intergenic 30.0%
! GAGAAGTGAAATTTGAGTTA+AGG + chr8.4:61257176-61257195 None:intergenic 30.0%
! GTGATATCATTTTCACAACA+AGG - chr8.4:61255008-61255027 MS.gene23562:intron 30.0%
! TATAAGTGATCCTTCTTTTG+AGG + chr8.4:61256942-61256961 None:intergenic 30.0%
! TGCTTTTAAACAGTAACAGA+GGG + chr8.4:61254175-61254194 None:intergenic 30.0%
! TGTTTATAAACAGTAACGGA+GGG - chr8.4:61254419-61254438 MS.gene23562:intron 30.0%
! TTTCTCAGCACTATTAGTTT+GGG + chr8.4:61257106-61257125 None:intergenic 30.0%
! TTTTCTTGGTTTATTTCAGG+TGG - chr8.4:61256963-61256982 MS.gene23562:CDS 30.0%
!! AAATTTGTGATGGATCATCT+AGG - chr8.4:61253423-61253442 MS.gene23562:intron 30.0%
!! AGCTGCTATTTGATTTTAGT+AGG - chr8.4:61255593-61255612 MS.gene23562:intron 30.0%
!! ATGAATTTAGGCTTTAAGCA+TGG + chr8.4:61255170-61255189 None:intergenic 30.0%
!! CATTGATTTAATGCTCTTGT+TGG - chr8.4:61253465-61253484 MS.gene23562:intron 30.0%
!! CTATTTTGAAAACCTCTTTG+AGG - chr8.4:61255633-61255652 MS.gene23562:intron 30.0%
!! CTTTTGCTTTTAAACAGTAG+CGG - chr8.4:61254622-61254641 MS.gene23562:intron 30.0%
!! TTGCTTTTAAACAGTAACAG+AGG + chr8.4:61254176-61254195 None:intergenic 30.0%
!!! TCCTGCTTTCTTTTGTTTTA+TGG - chr8.4:61255752-61255771 MS.gene23562:intron 30.0%
!!! TTTTTTCTTCCTCTGTTGTA+TGG - chr8.4:61255709-61255728 MS.gene23562:intron 30.0%
!!! TTTTTTGTTGGTACTTTTGC+AGG - chr8.4:61254778-61254797 MS.gene23562:intron 30.0%
ACAAACATGAACACTGTACA+TGG - chr8.4:61256808-61256827 MS.gene23562:intron 35.0%
ACCTAGAAAGTTGTAAACTC+GGG + chr8.4:61253777-61253796 None:intergenic 35.0%
AGAAACCATCAAGCAATGTT+AGG + chr8.4:61255681-61255700 None:intergenic 35.0%
AGGAAGGTAAGAATAAAGGA+CGG + chr8.4:61254892-61254911 None:intergenic 35.0%
ATACCTTAATAAGCGCGAAA+TGG - chr8.4:61254389-61254408 MS.gene23562:intron 35.0%
ATCCTTTCACTCTTTACACT+TGG - chr8.4:61256370-61256389 MS.gene23562:intron 35.0%
ATGGAACCGTTAAGAAGAAA+AGG - chr8.4:61253253-61253272 MS.gene23562:CDS 35.0%
CTTCTAAATATGCTCTTGCA+GGG - chr8.4:61256178-61256197 MS.gene23562:intron 35.0%
CTTTCTCAGCACTATTAGTT+TGG + chr8.4:61257107-61257126 None:intergenic 35.0%
GAAACCATCAAGCAATGTTA+GGG + chr8.4:61255680-61255699 None:intergenic 35.0%
GATGCAACACTTAAGATTGA+AGG + chr8.4:61256917-61256936 None:intergenic 35.0%
GATTTAATGCTCTTGTTGGT+TGG - chr8.4:61253469-61253488 MS.gene23562:intron 35.0%
GCTTAGGAAGGTAAGAATAA+AGG + chr8.4:61254896-61254915 None:intergenic 35.0%
TAAACCCTAACATTGCTTGA+TGG - chr8.4:61255673-61255692 MS.gene23562:intron 35.0%
TACAGTGTTCATGTTTGTGT+CGG + chr8.4:61256806-61256825 None:intergenic 35.0%
TAGTCTGAGCTGATATTTGA+TGG - chr8.4:61256057-61256076 MS.gene23562:intron 35.0%
TCAAATATCAGCTCAGACTA+TGG + chr8.4:61256057-61256076 None:intergenic 35.0%
TCCTTTCACTCTTTACACTT+GGG - chr8.4:61256371-61256390 MS.gene23562:intron 35.0%
TCTTCTAAATATGCTCTTGC+AGG - chr8.4:61256177-61256196 MS.gene23562:intron 35.0%
TGGAACCGTTAAGAAGAAAA+GGG - chr8.4:61253254-61253273 MS.gene23562:CDS 35.0%
TGGATCATCTAGGTAATTGT+TGG - chr8.4:61253433-61253452 MS.gene23562:intron 35.0%
TGGATTGAAGGAAAGTAGTT+CGG + chr8.4:61253633-61253652 None:intergenic 35.0%
TTTCGCGCTTATTAAGGTAT+CGG + chr8.4:61254389-61254408 None:intergenic 35.0%
! AACTAATAGTGCTGAGAAAG+AGG - chr8.4:61257107-61257126 MS.gene23562:CDS 35.0%
! ACTTTTGCAGGTGAAATGAA+TGG - chr8.4:61254790-61254809 MS.gene23562:intron 35.0%
! CTTTTGCAGGTGAAATGAAT+GGG - chr8.4:61254791-61254810 MS.gene23562:intron 35.0%
! GATGAGAAGTCTTTAGAAGA+AGG + chr8.4:61253357-61253376 None:intergenic 35.0%
! TCACCATTTCACACTTGTTA+AGG + chr8.4:61254602-61254621 None:intergenic 35.0%
! TCTACCCCTTTTCTTCTTAA+CGG + chr8.4:61253262-61253281 None:intergenic 35.0%
! TTCTCAGCACTATTAGTTTG+GGG + chr8.4:61257105-61257124 None:intergenic 35.0%
! TTTGAGATACTTTCCTTGTC+TGG - chr8.4:61256205-61256224 MS.gene23562:intron 35.0%
!! GAGATCGTTTTTGTGTAAGT+AGG - chr8.4:61256753-61256772 MS.gene23562:intron 35.0%
!! TGCTATACTCTTTGGTTTGT+AGG - chr8.4:61254971-61254990 MS.gene23562:intron 35.0%
!!! TCCTTGTTTTCAGATCTTGA+TGG + chr8.4:61257014-61257033 None:intergenic 35.0%
AAGGAATGAAGTTCCAAACC+AGG - chr8.4:61253122-61253141 MS.gene23562:CDS 40.0%
ACCCAAGTGTAAAGAGTGAA+AGG + chr8.4:61256375-61256394 None:intergenic 40.0%
ATGCCTACTGATACTAACCA+AGG - chr8.4:61256235-61256254 MS.gene23562:intron 40.0%
CATTTCATCACTGTCAATGC+CGG - chr8.4:61254841-61254860 MS.gene23562:intron 40.0%
CCTAGAAAGTTGTAAACTCG+GGG + chr8.4:61253776-61253795 None:intergenic 40.0%
CTTAAGTCCGCTATTGAGTT+TGG - chr8.4:61256087-61256106 MS.gene23562:intron 40.0%
GAAATAGGCAATGGTGACAA+TGG + chr8.4:61253321-61253340 None:intergenic 40.0%
GAAGGTAAGAATAAAGGACG+GGG + chr8.4:61254890-61254909 None:intergenic 40.0%
GAATTACAATGAGGGTAGTG+TGG - chr8.4:61254320-61254339 MS.gene23562:intron 40.0%
GAGGGCGAAAAAATTTGTGA+TGG - chr8.4:61253413-61253432 MS.gene23562:intron 40.0%
GATAAGACACATATTGCTCG+CGG + chr8.4:61255035-61255054 None:intergenic 40.0%
GCCATCAAGATCTGAAAACA+AGG - chr8.4:61257010-61257029 MS.gene23562:CDS 40.0%
GCGAGCAATATGTGTCTTAT+CGG - chr8.4:61255034-61255053 MS.gene23562:intron 40.0%
GCTGAAGATGAAATAGGCAA+TGG + chr8.4:61253330-61253349 None:intergenic 40.0%
GGAACCGTTAAGAAGAAAAG+GGG - chr8.4:61253255-61253274 MS.gene23562:CDS 40.0%
GGAAGGTAAGAATAAAGGAC+GGG + chr8.4:61254891-61254910 None:intergenic 40.0%
GGACATGTACTGCAAAATTC+TGG - chr8.4:61257147-61257166 MS.gene23562:CDS 40.0%
GTTCCTTGGTTAGTATCAGT+AGG + chr8.4:61256241-61256260 None:intergenic 40.0%
GTTGCATCTTCCTCAAAAGA+AGG - chr8.4:61256929-61256948 MS.gene23562:CDS 40.0%
TAACCAAGGAACAAGAAGCA+GGG - chr8.4:61256249-61256268 MS.gene23562:intron 40.0%
TACAGCTCCAAACTCAATAG+CGG + chr8.4:61256097-61256116 None:intergenic 40.0%
TAGTCAATAGTGGCTGATAG+CGG - chr8.4:61255514-61255533 MS.gene23562:CDS 40.0%
TCACCATTTCGCGCTTATTA+AGG + chr8.4:61254395-61254414 None:intergenic 40.0%
TCCTCTGTTGTATGGTTTCT+CGG - chr8.4:61255717-61255736 MS.gene23562:intron 40.0%
! ATATGTGTCTTATCGGCTAC+TGG - chr8.4:61255041-61255060 MS.gene23562:intron 40.0%
! GACATCTTTTGACCCAATAG+CGG + chr8.4:61253976-61253995 None:intergenic 40.0%
! GAGAAGTCTTTAGAAGAAGG+CGG + chr8.4:61253354-61253373 None:intergenic 40.0%
! GCATTGACAGTGATGAAATG+CGG + chr8.4:61254841-61254860 None:intergenic 40.0%
! GTTTGAAGTGTATCTCTGTC+TGG - chr8.4:61253553-61253572 MS.gene23562:intron 40.0%
! TCAAGATGGTGAGAAGCATA+TGG - chr8.4:61255373-61255392 MS.gene23562:intron 40.0%
! TCTTGGTTTATTTCAGGTGG+TGG - chr8.4:61256966-61256985 MS.gene23562:CDS 40.0%
! TGGTTTGGAACTTCATTCCT+TGG + chr8.4:61253123-61253142 None:intergenic 40.0%
!! AAGAGAAGGTATCAATCCTG+CGG - chr8.4:61253053-61253072 MS.gene23562:CDS 40.0%
!! GAGCTTTTGTTGTGGATTGA+AGG + chr8.4:61253645-61253664 None:intergenic 40.0%
!! TGCTTTTAAACAGTAGCGGA+GGG - chr8.4:61254626-61254645 MS.gene23562:intron 40.0%
!! TTGCTTTTAAACAGTAGCGG+AGG - chr8.4:61254625-61254644 MS.gene23562:intron 40.0%
!!! AAACCTCTTTGAGGGCATTT+TGG - chr8.4:61255642-61255661 MS.gene23562:intron 40.0%
!!! AACCTCTTTGAGGGCATTTT+GGG - chr8.4:61255643-61255662 MS.gene23562:intron 40.0%
ACAGGGGATGGAATACAAGA+GGG - chr8.4:61253395-61253414 MS.gene23562:intron 45.0%
ACCGAGAAACCATACAACAG+AGG + chr8.4:61255721-61255740 None:intergenic 45.0%
AGAAGAAAAGGGGTAGACCA+AGG - chr8.4:61253265-61253284 MS.gene23562:CDS 45.0%
AGGCATGAATGATCCAGACA+AGG + chr8.4:61256221-61256240 None:intergenic 45.0%
AGGGGTAGACCAAGGAAATA+TGG - chr8.4:61253273-61253292 MS.gene23562:CDS 45.0%
AGGTATCAATCCTGCGGTTA+CGG - chr8.4:61253059-61253078 MS.gene23562:CDS 45.0%
AGTCAGGAAATCAGCAGTGT+TGG - chr8.4:61255468-61255487 MS.gene23562:intron 45.0%
ATCCAAACCTACACTAACCG+GGG + chr8.4:61253235-61253254 None:intergenic 45.0%
CAAGGAAATATGGGCCAGAT+GGG - chr8.4:61253283-61253302 MS.gene23562:CDS 45.0%
CATCCAAACCTACACTAACC+GGG + chr8.4:61253236-61253255 None:intergenic 45.0%
CCATCCAAACCTACACTAAC+CGG + chr8.4:61253237-61253256 None:intergenic 45.0%
CCCCGAGTTTACAACTTTCT+AGG - chr8.4:61253773-61253792 MS.gene23562:intron 45.0%
CTAACCAAGGAACAAGAAGC+AGG - chr8.4:61256248-61256267 MS.gene23562:intron 45.0%
CTACTTCAGGGTTATGAACC+TGG + chr8.4:61253143-61253162 None:intergenic 45.0%
CTATTTGTCACACCGCTATC+CGG - chr8.4:61255970-61255989 MS.gene23562:intron 45.0%
CTGTGGTTTCACTTGCTTCA+CGG + chr8.4:61253379-61253398 None:intergenic 45.0%
GAAAGGATGTTTACCTGCAC+AGG + chr8.4:61256358-61256377 None:intergenic 45.0%
GAAATGAATGGGCACTCTGA+TGG - chr8.4:61254802-61254821 MS.gene23562:intron 45.0%
GATTTACTGACGCACCTCAT+AGG + chr8.4:61255120-61255139 None:intergenic 45.0%
GGGGTAGACCAAGGAAATAT+GGG - chr8.4:61253274-61253293 MS.gene23562:CDS 45.0%
GTAAGAATAAAGGACGGGGA+TGG + chr8.4:61254886-61254905 None:intergenic 45.0%
GTGAAGCAAGTGAAACCACA+GGG - chr8.4:61253378-61253397 MS.gene23562:intron 45.0%
TAGTAGTCAATCCCGGATAG+CGG + chr8.4:61255985-61256004 None:intergenic 45.0%
TATGCCGGACACAAGAAAAG+CGG - chr8.4:61257242-61257261 MS.gene23562:CDS 45.0%
TATTTGTCACACCGCTATCC+GGG - chr8.4:61255971-61255990 MS.gene23562:intron 45.0%
TCAGGGTTATGAACCTGGTT+TGG + chr8.4:61253138-61253157 None:intergenic 45.0%
TCCGTCATCCAGATTCTTCT+GGG - chr8.4:61255084-61255103 MS.gene23562:intron 45.0%
TGAAGCAAGTGAAACCACAG+GGG - chr8.4:61253379-61253398 MS.gene23562:intron 45.0%
TGCAATGGCCTGAGTAAATG+TGG + chr8.4:61257061-61257080 None:intergenic 45.0%
TGCATTCAAACATACCTCGC+CGG + chr8.4:61254863-61254882 None:intergenic 45.0%
! AAAGTACCCCCAGAAGAATC+TGG + chr8.4:61255095-61255114 None:intergenic 45.0%
! GGTTTATTTCAGGTGGTGGT+GGG - chr8.4:61256970-61256989 MS.gene23562:CDS 45.0%
! TCAGCACTATTAGTTTGGGG+TGG + chr8.4:61257102-61257121 None:intergenic 45.0%
! TGGTTTATTTCAGGTGGTGG+TGG - chr8.4:61256969-61256988 MS.gene23562:CDS 45.0%
!! TGTTTGTGTCGGTGCTGTAT+AGG + chr8.4:61256795-61256814 None:intergenic 45.0%
!!! ACCTCTTTGAGGGCATTTTG+GGG - chr8.4:61255644-61255663 MS.gene23562:intron 45.0%
AGCATGAGCACACGTGAACT+TGG + chr8.4:61256414-61256433 None:intergenic 50.0%
AGGTGGAATGAGTGTCTCAC+TGG - chr8.4:61256273-61256292 MS.gene23562:intron 50.0%
AGTTCACGTGTGCTCATGCT+CGG - chr8.4:61256414-61256433 MS.gene23562:intron 50.0%
ATGGCCTGAGTAAATGTGGC+TGG + chr8.4:61257057-61257076 None:intergenic 50.0%
CACAGGGGATGGAATACAAG+AGG - chr8.4:61253394-61253413 MS.gene23562:intron 50.0%
CCAAGGAAATATGGGCCAGA+TGG - chr8.4:61253282-61253301 MS.gene23562:CDS 50.0%
CCATCTGGCCCATATTTCCT+TGG + chr8.4:61253285-61253304 None:intergenic 50.0%
CCCCAGAAGAATCTGGATGA+CGG + chr8.4:61255088-61255107 None:intergenic 50.0%
CCGGTTAGTGTAGGTTTGGA+TGG - chr8.4:61253234-61253253 MS.gene23562:CDS 50.0%
CCGTCATCCAGATTCTTCTG+GGG - chr8.4:61255085-61255104 MS.gene23562:intron 50.0%
CGCCTTTCGAAGGGAAAACT+GGG - chr8.4:61257206-61257225 MS.gene23562:CDS 50.0%
CGTCATCCAGATTCTTCTGG+GGG - chr8.4:61255086-61255105 MS.gene23562:intron 50.0%
CGTGAAGCAAGTGAAACCAC+AGG - chr8.4:61253377-61253396 MS.gene23562:intron 50.0%
CTCCGTCATCCAGATTCTTC+TGG - chr8.4:61255083-61255102 MS.gene23562:intron 50.0%
CTCTTGTATTCCATCCCCTG+TGG + chr8.4:61253396-61253415 None:intergenic 50.0%
GATGTTTACCTGCACAGGAG+TGG + chr8.4:61256353-61256372 None:intergenic 50.0%
GCTGAGAAAGAGGATGCCAT+GGG - chr8.4:61257117-61257136 MS.gene23562:CDS 50.0%
GGCGGAGCTGAAGATGAAAT+AGG + chr8.4:61253336-61253355 None:intergenic 50.0%
GGTCAACATGCACTCTATGC+CGG - chr8.4:61257227-61257246 MS.gene23562:CDS 50.0%
TCCCCAAAATGCCCTCAAAG+AGG + chr8.4:61255648-61255667 None:intergenic 50.0%
TGATATGCGGATGGAGCTTC+TGG + chr8.4:61253093-61253112 None:intergenic 50.0%
TGCACCAGCCACATTTACTC+AGG - chr8.4:61257050-61257069 MS.gene23562:CDS 50.0%
TGCTGAGAAAGAGGATGCCA+TGG - chr8.4:61257116-61257135 MS.gene23562:CDS 50.0%
TTCCCCGGTTAGTGTAGGTT+TGG - chr8.4:61253230-61253249 MS.gene23562:CDS 50.0%
! AGGTGCTGCAGATACTGCAA+TGG + chr8.4:61257076-61257095 None:intergenic 50.0%
! CCAGTTTTCCCTTCGAAAGG+CGG + chr8.4:61257208-61257227 None:intergenic 50.0%
! CCTTGGTGCGACATGATATG+CGG + chr8.4:61253106-61253125 None:intergenic 50.0%
! GACCCAGTTTTCCCTTCGAA+AGG + chr8.4:61257211-61257230 None:intergenic 50.0%
! GCACTATTAGTTTGGGGTGG+CGG + chr8.4:61257099-61257118 None:intergenic 50.0%
! TGATACTGTCGGTGTTTCCC+CGG - chr8.4:61253215-61253234 MS.gene23562:CDS 50.0%
! TTCAGATCTTGATGGCCGCT+AGG + chr8.4:61257006-61257025 None:intergenic 50.0%
! TTTTCCCTTCGAAAGGCGGT+GGG + chr8.4:61257204-61257223 None:intergenic 50.0%
!! TGGGGGTACTTTGACCTATG+AGG - chr8.4:61255103-61255122 MS.gene23562:intron 50.0%
!! TTGGTGTCAGACACACGTGT+TGG - chr8.4:61256886-61256905 MS.gene23562:intron 50.0%
!!! GTAGCCGCTTTTCTTGTGTC+CGG + chr8.4:61257249-61257268 None:intergenic 50.0%
AAAGAGGATGCCATGGGTGG+CGG - chr8.4:61257123-61257142 MS.gene23562:CDS 55.0%
ACACCGACAGTATCAGCAGC+AGG + chr8.4:61253210-61253229 None:intergenic 55.0%
AGCAGCAGTAGATCCTCCTG+CGG + chr8.4:61253178-61253197 None:intergenic 55.0%
AGCGGTGCGGAACCAAATAG+CGG - chr8.4:61255563-61255582 MS.gene23562:intron 55.0%
CAAGGAACAAGAAGCAGGGC+AGG - chr8.4:61256253-61256272 MS.gene23562:intron 55.0%
CAGCTGAAGCAGCCGCTATT+TGG + chr8.4:61255578-61255597 None:intergenic 55.0%
CCGCATATCATGTCGCACCA+AGG - chr8.4:61253103-61253122 MS.gene23562:CDS 55.0%
CCGCCTTTCGAAGGGAAAAC+TGG - chr8.4:61257205-61257224 MS.gene23562:CDS 55.0%
CGCTACGGTAATCGTCCTAG+CGG - chr8.4:61255545-61255564 MS.gene23562:intron 55.0%
CGCTAGGACGATTACCGTAG+CGG + chr8.4:61255547-61255566 None:intergenic 55.0%
CTGCAGCTGTGGCTACTTCA+GGG + chr8.4:61253155-61253174 None:intergenic 55.0%
CTGCCCTGCTTCTTGTTCCT+TGG + chr8.4:61256255-61256274 None:intergenic 55.0%
GAGAAAGAGGATGCCATGGG+TGG - chr8.4:61257120-61257139 MS.gene23562:CDS 55.0%
GCAAGTGAAACCACAGGGGA+TGG - chr8.4:61253383-61253402 MS.gene23562:intron 55.0%
GCTCCTGCTGCTGATACTGT+CGG - chr8.4:61253204-61253223 MS.gene23562:CDS 55.0%
GGCATGTTGACAGACCCATC+TGG + chr8.4:61253300-61253319 None:intergenic 55.0%
GGTGCGACATGATATGCGGA+TGG + chr8.4:61253102-61253121 None:intergenic 55.0%
GGTGTTTCCCCGGTTAGTGT+AGG - chr8.4:61253225-61253244 MS.gene23562:CDS 55.0%
GTGGGAAGCTTTCTGCCTAG+CGG - chr8.4:61256988-61257007 MS.gene23562:CDS 55.0%
TATAGCTGCCACTCCTGTGC+AGG - chr8.4:61256342-61256361 MS.gene23562:intron 55.0%
! CTATTAGTTTGGGGTGGCGG+AGG + chr8.4:61257096-61257115 None:intergenic 55.0%
! CTATTTGGTTCCGCACCGCT+AGG + chr8.4:61255563-61255582 None:intergenic 55.0%
! GTTTTCCCTTCGAAAGGCGG+TGG + chr8.4:61257205-61257224 None:intergenic 55.0%
!! ATAGTGGCTGATAGCGGCGA+CGG - chr8.4:61255520-61255539 MS.gene23562:CDS 55.0%
AGCCCTGATGGTCGCGTAGT+AGG - chr8.4:61256298-61256317 MS.gene23562:intron 60.0%
CATCACTGTCAATGCCGGCG+AGG - chr8.4:61254846-61254865 MS.gene23562:intron 60.0%
CCTGATGGTCGCGTAGTAGG+CGG - chr8.4:61256301-61256320 MS.gene23562:intron 60.0%
CGCCTACTACGCGACCATCA+GGG + chr8.4:61256303-61256322 None:intergenic 60.0%
CGGTAATCGTCCTAGCGGTG+CGG - chr8.4:61255550-61255569 MS.gene23562:intron 60.0%
GCTGCAGCTGTGGCTACTTC+AGG + chr8.4:61253156-61253175 None:intergenic 60.0%
GGAACAAGAAGCAGGGCAGG+TGG - chr8.4:61256256-61256275 MS.gene23562:intron 60.0%
GTAGTAGGCGGTGGAGTTGC+TGG - chr8.4:61256313-61256332 MS.gene23562:intron 60.0%
GTATCAGCAGCAGGAGCAGC+AGG + chr8.4:61253201-61253220 None:intergenic 60.0%
GTCTCACTGGCAAGCCCTGA+TGG - chr8.4:61256286-61256305 MS.gene23562:intron 60.0%
TACATGTCCACCGCCACCCA+TGG + chr8.4:61257136-61257155 None:intergenic 60.0%
TCCACCCACCGCCTTTCGAA+GGG - chr8.4:61257197-61257216 MS.gene23562:CDS 60.0%
ATAGCGGCGACGGACCGCTA+CGG - chr8.4:61255530-61255549 MS.gene23562:intron 65.0%
CCGCCTACTACGCGACCATC+AGG + chr8.4:61256304-61256323 None:intergenic 65.0%
CTCCACCCACCGCCTTTCGA+AGG - chr8.4:61257196-61257215 MS.gene23562:CDS 65.0%
CTTCGAAAGGCGGTGGGTGG+AGG + chr8.4:61257198-61257217 None:intergenic 65.0%
GATGGTCGCGTAGTAGGCGG+TGG - chr8.4:61256304-61256323 MS.gene23562:intron 65.0%
GGCGCTATCACCGTAACCGC+AGG + chr8.4:61253072-61253091 None:intergenic 65.0%
TCCCTTCGAAAGGCGGTGGG+TGG + chr8.4:61257201-61257220 None:intergenic 65.0%
GTAGCCACAGCTGCAGCCGC+AGG - chr8.4:61253159-61253178 MS.gene23562:CDS 70.0%
TCCTCCTGCGGCTGCAGCTG+TGG + chr8.4:61253166-61253185 None:intergenic 70.0%
! GAGGATGCCATGGGTGGCGG+TGG - chr8.4:61257126-61257145 MS.gene23562:CDS 70.0%
GCCACAGCTGCAGCCGCAGG+AGG - chr8.4:61253162-61253181 MS.gene23562:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 61253045 61257299 61253045 ID=MS.gene23562
chr8.4 mRNA 61253045 61257299 61253045 ID=MS.gene23562.t1;Parent=MS.gene23562
chr8.4 exon 61256900 61257299 61256900 ID=MS.gene23562.t1.exon1;Parent=MS.gene23562.t1
chr8.4 CDS 61256900 61257299 61256900 ID=cds.MS.gene23562.t1;Parent=MS.gene23562.t1
chr8.4 exon 61255477 61255544 61255477 ID=MS.gene23562.t1.exon2;Parent=MS.gene23562.t1
chr8.4 CDS 61255477 61255544 61255477 ID=cds.MS.gene23562.t1;Parent=MS.gene23562.t1
chr8.4 exon 61255220 61255351 61255220 ID=MS.gene23562.t1.exon3;Parent=MS.gene23562.t1
chr8.4 CDS 61255220 61255351 61255220 ID=cds.MS.gene23562.t1;Parent=MS.gene23562.t1
chr8.4 exon 61253981 61254145 61253981 ID=MS.gene23562.t1.exon4;Parent=MS.gene23562.t1
chr8.4 CDS 61253981 61254145 61253981 ID=cds.MS.gene23562.t1;Parent=MS.gene23562.t1
chr8.4 exon 61253045 61253362 61253045 ID=MS.gene23562.t1.exon5;Parent=MS.gene23562.t1
chr8.4 CDS 61253045 61253362 61253045 ID=cds.MS.gene23562.t1;Parent=MS.gene23562.t1
Gene Sequence

>MS.gene23562

ATGGAAGGAAGAGAAGGTATCAATCCTGCGGTTACGGTGATAGCGCCAGAAGCTCCATCCGCATATCATGTCGCACCAAGGAATGAAGTTCCAAACCAGGTTCATAACCCTGAAGTAGCCACAGCTGCAGCCGCAGGAGGATCTACTGCTGCTCCTGCTGCTCCTGCTGCTGATACTGTCGGTGTTTCCCCGGTTAGTGTAGGTTTGGATGGAACCGTTAAGAAGAAAAGGGGTAGACCAAGGAAATATGGGCCAGATGGGTCTGTCAACATGCCATTGTCACCATTGCCTATTTCATCTTCAGCTCCGCCTTCTTCTAAAGACTTCTCATCCGTGAAGCAAGTGAAACCACAGGGGATGGAATACAAGAGGGCGAAAAAATTTGTGATGGATCATCTAGGTAATTGTTGGTCCTTTATACATTGATTTAATGCTCTTGTTGGTTGGTAAATATTATAGTTAATCATATGACTTTCATTTTGTATGTAACCTTTTTCCTTTTGTTGATGTTTGAAGTGTATCTCTGTCTGGTATCAAAAATACTTTGCTTCTCTTGCTTCTTTGAGTTTGCATGCTATTTTACTTCCGAACTACTTTCCTTCAATCCACAACAAAAGCTCTCAAATTTGTTTTTATACAGCTTATTTATGACTTATGCAAGAATTAATTAAATCTGTCCATATTCCTGACCTTTAATTGTACAACTTCATAGTGTGCTTGTTAATATTCCCCGAGTTTACAACTTTCTAGGTTAGTGCTAATGAATTACGCATTCAATGTATTTATTGTACACCGTTATAAATACAAGTTGTGTAGATACAAAGAATTTACAGAAATTACAACTTTATAGATTTTATTGCTTACTTTCTGATTTTCAAATGAATTCAAAAACATCTTTTGGTTGTATTTCTGTAAGAATTAATTAATTCCGCTATTGGGTCAAAAGATGTCTTTTTTTGTCAAGTTTAATTAATTCTTACAGAAATACAAACAAAAGATATTTCTGAATTCATTTGAAAATCAGAAAGTAAACACTAAATTCTATAAAGTTGTAGTTTAAGTAAATTCTATGTATCAAGGCAACTTGTATTTATAATGGTGTACAATAAATACATTGAATGCGTACTCCCTCTGTTACTGTTTAAAAGCAAAAGTCACCATTTCACACTTATTAAGGTATGCCGTTGCTTATATTAATTTCAGTATAAAAACATAATTGCTCTACCCATAATACCCTCATTGTAATTTGTAAAGAATTACGTTTTAATTTGTAAAGAATTACAATGAGGGTAGTGTGGCTAAAGCAATTATGTTTTTGACTGAAATTAATATAAGCAAAGGCCGATACCTTAATAAGCGCGAAATGGTGACTTTTGTTTATAAACAGTAACGGAGGGAGTACACATTCAATGTATTTATTGTACACCATTATAAAAACAAGTTGTGTAGATACAAAGAATTTACAGAAATTACGACATTTCGCAAATTACAATGAGGGTATTTTGGGTAAAGCAATTATGTTTTTTTACTGAAATTAATATAAGCAACGGCATACCTTAACAAGTGTGAAATGGTGACTTTTGCTTTTAAACAGTAGCGGAGGGAGTATGCATTCAATGTATTCATTGTACACCATTATAAATACAAGTTGCCTTGATACATAGAATTTACTTAAACTACAACTTTATAGAATTTAGTGTTTACTTTCTGATTTTCAAATGAATTCAGAAATATTTTTTGTTGGTACTTTTGCAGGTGAAATGAATGGGCACTCTGATGGTACAAACTTTATGCCGCATTTCATCACTGTCAATGCCGGCGAGGTATGTTTGAATGCACCATCCCCGTCCTTTATTCTTACCTTCCTAAGCGTTATACAAATTGATGATACCATTACTTCCAAAGTTATAATGAATAATATTATCTTGCTATACTCTTTGGTTTGTAGGATATTACAAATAAGGTGATATCATTTTCACAACAAGGGCCGCGAGCAATATGTGTCTTATCGGCTACTGGAGTGATTTCAAATGTGACTCTCCGTCATCCAGATTCTTCTGGGGGTACTTTGACCTATGAGGTGCGTCAGTAAATCATGACACTGACTTATTTTTATTTTATTCCATGCTTAAAGCCTAAATTCATTTGAAGCTTCTGTTTAACACTTTGTTCTGTTTTATGTAAAGTATGGATCTAAGGTTTAGGTATTGCAATAATCATCGGATTGATAATATTTATAATCAGGACTGAAAAATTGAAATATTATTAATGATGTTTTTTGTTTATATGAGAATGGTTTTATAAACATTTTTCTGTTCAGTAAATTAATCAAGATGGTGAGAAGCATATGGTTTTCAATGATTTTGTAAATTAATCAAGATGGTTTTATGAACATTTTTCTGTACAATTCTACTAATGTATTGAGTCAGGAAATCAGCAGTGTTGGTAGTTGATATGTTATATGTTCAGTAGTCAATAGTGGCTGATAGCGGCGACGGACCGCTACGGTAATCGTCCTAGCGGTGCGGAACCAAATAGCGGCTGCTTCAGCTGCTATTTGATTTTAGTAGGAAATTTCCAAATTGACCCTATTTTGAAAACCTCTTTGAGGGCATTTTGGGGATTCTGTAAACCCTAACATTGCTTGATGGTTTCTCTCTGTTTTTTTTTCTTCCTCTGTTGTATGGTTTCTCGGTTCTTTTTTTCTTCCTGCTTTCTTTTGTTTTATGGTTTATCTTGTTTTATGATTTTCTTGTTTGTTCCTCTGTTTTTTGTTTTTTGGTGTTTTGATTTTTGACTGTTTCATGAGAGTGTTATATTTAATTTTATATAAGTTATTTGTTTTGAGAAATATACAAGTTTGTTTATAATTTAACAGTATTATTCATGTAATTTGTCCAAAACATAATCAAATATCGATGACTGCTATTTGTCACACCGCTATCCGGGATTGACTACTATTATGTTGTTACTAGTAGTTGAAATGACTTTCACAAAAGTCTGGTTTTGCCATAGTCTGAGCTGATATTTGATGGTTATTTTCTTAAGTCCGCTATTGAGTTTGGAGCTGTAGTTTTTCTGAATGTTGATGTATTGATATTGGTGCCACAATATCTTCTGTTTAATATTTCTTCTTCTAAATATGCTCTTGCAGGGTCGTTTTGAGATACTTTCCTTGTCTGGATCATTCATGCCTACTGATACTAACCAAGGAACAAGAAGCAGGGCAGGTGGAATGAGTGTCTCACTGGCAAGCCCTGATGGTCGCGTAGTAGGCGGTGGAGTTGCTGGTGTTCTTATAGCTGCCACTCCTGTGCAGGTAAACATCCTTTCACTCTTTACACTTGGGTCATGTTTAACAATGCACCAAGTTCACGTGTGCTCATGCTCGGAAAGTGAACCACCATTTTCCCTTAATATAGTTGCCAAACAAATAGGATTGAGAATTGAGAAGTGACTATTTTAACGTCTATTTGCTTTGAGACATCTGTTTTCACTTTTAATTACAAAATTGCTACATTGTTTTCATTTTTCATCTCCTTTCCAAATATTTCACTGTTTCCTATACAAATCTTTGTAAACAGAAAGCAAATTGAAAACAATGCGACAGTTTTGTAATTAAAAGTGAAAAGTCAATAAAAATAGAAAATGTCTTCTCATACTAAATAGGCCCTAAACTTGCAATTCTATGTCTCAACAATTGCACTAGAGATCGTTTTTGTGTAAGTAGGCCAAGTTAACATGATTCCTATACAGCACCGACACAAACATGAACACTGTACATGGCTCTGACACGTGTAATAATTTGAGAAAATGAAATAATTAAATTCAATCACATGTGTTGGTGTCAGACACACGTGTTGGATGTGCCTTCAATCTTAAGTGTTGCATCTTCCTCAAAAGAAGGATCACTTATATTTTTCTTGGTTTATTTCAGGTGGTGGTGGGAAGCTTTCTGCCTAGCGGCCATCAAGATCTGAAAACAAGGAAGCCCAAGTCTGATTATGCACCAGCCACATTTACTCAGGCCATTGCAGTATCTGCAGCACCTCCGCCACCCCAAACTAATAGTGCTGAGAAAGAGGATGCCATGGGTGGCGGTGGACATGTACTGCAAAATTCTGGAACCCTTAACTCAAATTTCACTTCTCCTCCACCCACCGCCTTTCGAAGGGAAAACTGGGTCAACATGCACTCTATGCCGGACACAAGAAAAGCGGCTACTGATATTAACATATCTTTGCCTGATAATTAA
Protein sequence

>MS.gene23562.t1

MEGREGINPAVTVIAPEAPSAYHVAPRNEVPNQVHNPEVATAAAAGGSTAAPAAPAADTVGVSPVSVGLDGTVKKKRGRPRKYGPDGSVNMPLSPLPISSSAPPSSKDFSSVKQVKPQGMEYKRAKKFVMDHLGEMNGHSDGTNFMPHFITVNAGEDITNKVISFSQQGPRAICVLSATGVISNVTLRHPDSSGGTLTYEGRFEILSLSGSFMPTDTNQGTRSRAGGMSVSLASPDGRVVGGGVAGVLIAATPVQVVVGSFLPSGHQDLKTRKPKSDYAPATFTQAIAVSAAPPPPQTNSAEKEDAMGGGGHVLQNSGTLNSNFTSPPPTAFRRENWVNMHSMPDTRKAATDINISLPDN