Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23680.t1 | RHN39987.1 | 100 | 64 | 0 | 0 | 1 | 64 | 111 | 174 | 3.80E-26 | 126.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23680.t1 | A4UHT7 | 57.4 | 54 | 23 | 0 | 11 | 64 | 133 | 186 | 7.8e-12 | 70.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23680.t1 | G7LBU2 | 100.0 | 64 | 0 | 0 | 1 | 64 | 111 | 174 | 2.8e-26 | 126.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049499 | MS.gene23680 | 0.81053 | 1.10E-50 | -1.69E-46 |
| MS.gene051637 | MS.gene23680 | 0.800081 | 1.70E-48 | -1.69E-46 |
| MS.gene05952 | MS.gene23680 | 0.827378 | 1.62E-54 | -1.69E-46 |
| MS.gene060935 | MS.gene23680 | 0.816355 | 5.77E-52 | -1.69E-46 |
| MS.gene060937 | MS.gene23680 | 0.832567 | 8.78E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23680.t1 | MTR_8g032030 | 100.000 | 64 | 0 | 0 | 1 | 64 | 111 | 174 | 3.12e-41 | 134 |
| MS.gene23680.t1 | MTR_8g031990 | 82.812 | 64 | 10 | 1 | 1 | 64 | 111 | 173 | 2.29e-30 | 107 |
| MS.gene23680.t1 | MTR_8g032000 | 81.250 | 64 | 11 | 1 | 1 | 64 | 111 | 173 | 3.03e-29 | 104 |
| MS.gene23680.t1 | MTR_8g032020 | 76.562 | 64 | 15 | 0 | 1 | 64 | 112 | 175 | 1.16e-27 | 100 |
| MS.gene23680.t1 | MTR_8g032010 | 76.562 | 64 | 14 | 1 | 1 | 64 | 111 | 173 | 3.60e-27 | 99.4 |
| MS.gene23680.t1 | MTR_8g031980 | 63.333 | 60 | 22 | 0 | 5 | 64 | 114 | 173 | 2.35e-21 | 84.0 |
| MS.gene23680.t1 | MTR_8g031960 | 64.407 | 59 | 21 | 0 | 6 | 64 | 116 | 174 | 3.65e-21 | 83.6 |
| MS.gene23680.t1 | MTR_8g031940 | 65.000 | 60 | 21 | 0 | 5 | 64 | 114 | 173 | 1.15e-20 | 82.4 |
| MS.gene23680.t1 | MTR_8g031900 | 59.184 | 49 | 20 | 0 | 16 | 64 | 120 | 168 | 1.10e-14 | 66.2 |
| MS.gene23680.t1 | MTR_8g031910 | 60.784 | 51 | 20 | 0 | 14 | 64 | 112 | 162 | 2.49e-14 | 65.1 |
| MS.gene23680.t1 | MTR_8g031650 | 58.824 | 51 | 21 | 0 | 14 | 64 | 215 | 265 | 6.75e-13 | 61.6 |
| MS.gene23680.t1 | MTR_8g031630 | 58.824 | 51 | 21 | 0 | 14 | 64 | 121 | 171 | 7.96e-13 | 60.8 |
| MS.gene23680.t1 | MTR_8g031600 | 56.863 | 51 | 22 | 0 | 14 | 64 | 121 | 171 | 8.24e-13 | 60.8 |
| MS.gene23680.t1 | MTR_8g031650 | 58.824 | 51 | 21 | 0 | 14 | 64 | 121 | 171 | 8.62e-13 | 61.2 |
| MS.gene23680.t1 | MTR_8g031650 | 58.824 | 51 | 21 | 0 | 14 | 64 | 381 | 431 | 8.62e-13 | 61.2 |
| MS.gene23680.t1 | MTR_7g076665 | 48.148 | 54 | 28 | 0 | 11 | 64 | 117 | 170 | 9.65e-13 | 60.8 |
| MS.gene23680.t1 | MTR_5g083430 | 55.319 | 47 | 21 | 0 | 18 | 64 | 13 | 59 | 1.56e-12 | 58.9 |
| MS.gene23680.t1 | MTR_8g031610 | 54.902 | 51 | 23 | 0 | 14 | 64 | 119 | 169 | 3.11e-12 | 59.3 |
| MS.gene23680.t1 | MTR_7g076655 | 48.980 | 49 | 25 | 0 | 16 | 64 | 113 | 161 | 2.56e-11 | 56.6 |
| MS.gene23680.t1 | MTR_7g076660 | 48.980 | 49 | 25 | 0 | 16 | 64 | 113 | 161 | 5.93e-11 | 55.8 |
| MS.gene23680.t1 | MTR_7g076660 | 48.980 | 49 | 25 | 0 | 16 | 64 | 114 | 162 | 8.96e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23680.t1 | AT2G24190 | 63.265 | 49 | 18 | 0 | 16 | 64 | 122 | 170 | 7.10e-16 | 69.3 |
| MS.gene23680.t1 | AT2G24190 | 63.265 | 49 | 18 | 0 | 16 | 64 | 127 | 175 | 7.36e-16 | 69.3 |
| MS.gene23680.t1 | AT3G61220 | 52.459 | 61 | 29 | 0 | 4 | 64 | 110 | 170 | 1.19e-15 | 68.9 |
| MS.gene23680.t1 | AT3G61220 | 52.459 | 61 | 29 | 0 | 4 | 64 | 117 | 177 | 1.23e-15 | 68.9 |
| MS.gene23680.t1 | AT3G61220 | 52.459 | 61 | 29 | 0 | 4 | 64 | 141 | 201 | 1.56e-15 | 68.6 |
| MS.gene23680.t1 | AT1G01800 | 47.059 | 51 | 27 | 0 | 14 | 64 | 85 | 135 | 9.03e-13 | 60.5 |
| MS.gene23680.t1 | AT1G01800 | 47.059 | 51 | 27 | 0 | 14 | 64 | 120 | 170 | 1.48e-12 | 60.5 |
Find 0 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATTCAAATAAATTATTATG+GGG | - | 54868:15098-15117 | MS.gene23680:intron | 15.0% |
| !! | GCATTCAAATAAATTATTAT+GGG | - | 54868:15097-15116 | MS.gene23680:intron | 15.0% |
| !! | TGCATTCAAATAAATTATTA+TGG | - | 54868:15096-15115 | MS.gene23680:intron | 15.0% |
| !!! | AAATTTTTTGATAAGTTTAG+TGG | + | 54868:14773-14792 | MS.gene23680:intergenic | 15.0% |
| !!! | AATTTTTTGATAAGTTTAGT+GGG | + | 54868:14772-14791 | MS.gene23680:intergenic | 15.0% |
| !! | ATACATTCTTAACAAAAATG+TGG | + | 54868:14804-14823 | MS.gene23680:intergenic | 20.0% |
| ! | GTTCAAAATGATCAAGATAA+TGG | + | 54868:14682-14701 | MS.gene23680:intergenic | 25.0% |
| ! | TTCTTAACAAAAATGTGGTT+TGG | + | 54868:14799-14818 | MS.gene23680:intergenic | 25.0% |
| ! | TTTAGTGGGATATTGAAATT+TGG | + | 54868:14758-14777 | MS.gene23680:intergenic | 25.0% |
| !!! | TTGAAATTTGGTGTTTTGAT+TGG | + | 54868:14746-14765 | MS.gene23680:intergenic | 25.0% |
| AATGGACGAGATAAATAACA+AGG | + | 54868:14664-14683 | MS.gene23680:intergenic | 30.0% | |
| ATGGACGAGATAAATAACAA+GGG | + | 54868:14663-14682 | MS.gene23680:intergenic | 30.0% | |
| GGTTTGAAGAATTTCGATAA+TGG | - | 54868:14979-14998 | MS.gene23680:intron | 30.0% | |
| TATGTTAACTGTGTCAAAAC+TGG | - | 54868:14958-14977 | MS.gene23680:intron | 30.0% | |
| ! | ATCCTGAAATTTGAAACTAG+TGG | - | 54868:14822-14841 | MS.gene23680:intron | 30.0% |
| ! | CATGACAATTTTATGTAGTG+AGG | + | 54868:14866-14885 | MS.gene23680:intergenic | 30.0% |
| ! | TCCTGAAATTTGAAACTAGT+GGG | - | 54868:14823-14842 | MS.gene23680:intron | 30.0% |
| ! | TCTTGTGAAATTTGAAACTG+TGG | - | 54868:14914-14933 | MS.gene23680:intron | 30.0% |
| ! | TTTTATGTAGTGAGGTAGTT+TGG | + | 54868:14858-14877 | MS.gene23680:intergenic | 30.0% |
| ATCTGATGAAGAATCGAGAA+GGG | - | 54868:15041-15060 | MS.gene23680:intron | 35.0% | |
| TATCTGATGAAGAATCGAGA+AGG | - | 54868:15040-15059 | MS.gene23680:intron | 35.0% | |
| TGAATCAGATAGTTGAAGGA+GGG | + | 54868:15151-15170 | MS.gene23680:intergenic | 35.0% | |
| TTGGTGAATCAGATAGTTGA+AGG | + | 54868:15155-15174 | MS.gene23680:intergenic | 35.0% | |
| ! | GATGATACATTGACTACTCT+TGG | + | 54868:15174-15193 | MS.gene23680:intergenic | 35.0% |
| ! | TCCCACTAGTTTCAAATTTC+AGG | + | 54868:14827-14846 | MS.gene23680:intergenic | 35.0% |
| ! | TGTGAAATTTGAAACTGTGG+TGG | - | 54868:14917-14936 | MS.gene23680:intron | 35.0% |
| ! | TTGAGTTGTTCTTACTTTGC+AGG | - | 54868:15011-15030 | MS.gene23680:intron | 35.0% |
| AACATCCATGATGCATGTAC+TGG | - | 54868:14699-14718 | MS.gene23680:CDS | 40.0% | |
| ACATCCATGATGCATGTACT+GGG | - | 54868:14700-14719 | MS.gene23680:CDS | 40.0% | |
| AGTTGTTCTTACTTTGCAGG+CGG | - | 54868:15014-15033 | MS.gene23680:intron | 40.0% | |
| GAATCAGATAGTTGAAGGAG+GGG | + | 54868:15150-15169 | MS.gene23680:intergenic | 40.0% | |
| GTGAATCAGATAGTTGAAGG+AGG | + | 54868:15152-15171 | MS.gene23680:intergenic | 40.0% | |
| AAGACCCAGTACATGCATCA+TGG | + | 54868:14707-14726 | MS.gene23680:intergenic | 45.0% | |
| ATAGTTGAAGGAGGGGAAGA+AGG | + | 54868:15143-15162 | MS.gene23680:intergenic | 45.0% | |
| GTCAATGTATCATCGAGAGC+AGG | - | 54868:15180-15199 | MS.gene23680:intron | 45.0% | |
| ATCATCGAGAGCAGGCACGA+TGG | - | 54868:15188-15207 | MS.gene23680:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 54868 | gene | 14631 | 15212 | 14631 | ID=MS.gene23680 |
| 54868 | mRNA | 14631 | 15212 | 14631 | ID=MS.gene23680.t1;Parent=MS.gene23680 |
| 54868 | exon | 15201 | 15212 | 15201 | ID=MS.gene23680.t1.exon1;Parent=MS.gene23680.t1 |
| 54868 | CDS | 15201 | 15212 | 15201 | ID=cds.MS.gene23680.t1;Parent=MS.gene23680.t1 |
| 54868 | exon | 14631 | 14810 | 14631 | ID=MS.gene23680.t1.exon2;Parent=MS.gene23680.t1 |
| 54868 | CDS | 14631 | 14810 | 14631 | ID=cds.MS.gene23680.t1;Parent=MS.gene23680.t1 |
| Gene Sequence |
| Protein sequence |
