AlfalfaGEDB | MS.gene25093

M. sativa cultivar XinJiangDaYe / MS.gene25093

KEGG: K13464
GO: -
PFAM: PF06200.17 PF09425.13

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25093.t1	XP_013444474.1	95.3	235	11	0	1	235	1	235	1.40E-118	435.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25093.t1	Q9C9E3	34.1	220	115	5	15	212	3	214	1.6e-17	91.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25093.t1	I3T425	95.3	235	11	0	1	235	1	235	1.0e-118	435.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene25093.t1	TF	Tify

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051083	MS.gene25093	0.822964	1.79E-53	-1.69E-46
MS.gene057422	MS.gene25093	0.821867	3.21E-53	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25093.t1	MTR_8g021380	95.319	235	11	0	1	235	1	235	8.38e-167	459
MS.gene25093.t1	MTR_8g021380	78.838	241	10	1	1	200	1	241	1.22e-130	369
MS.gene25093.t1	MTR_2g042900	32.900	231	118	7	11	210	12	236	1.34e-22	92.8
MS.gene25093.t1	MTR_2g042900	28.685	251	112	7	11	200	12	256	1.97e-14	70.9
MS.gene25093.t1	MTR_5g013530	33.071	127	70	4	85	208	24	138	6.03e-14	67.8
MS.gene25093.t1	MTR_5g013530	33.884	121	65	4	91	208	8	116	1.19e-13	66.6
MS.gene25093.t1	MTR_5g013520	32.258	124	63	3	91	208	1	109	1.64e-12	63.5
MS.gene25093.t1	MTR_4g124960	30.693	202	100	7	11	208	12	177	3.83e-12	63.5
MS.gene25093.t1	MTR_5g013515	31.034	116	77	1	93	208	2	114	4.18e-12	62.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25093.t1	AT1G72450	35.000	220	113	7	15	212	3	214	3.48e-24	97.4
MS.gene25093.t1	AT1G19180	33.036	224	115	8	12	209	14	228	4.68e-19	83.6
MS.gene25093.t1	AT1G19180	33.036	224	115	8	12	209	14	228	4.68e-19	83.6
MS.gene25093.t1	AT1G17380	33.180	217	110	8	17	212	11	213	1.40e-18	82.4
MS.gene25093.t1	AT1G72450	33.173	208	109	6	15	200	3	202	3.76e-18	80.1
MS.gene25093.t1	AT1G74950	29.600	250	142	9	1	223	1	243	1.20e-16	77.0
MS.gene25093.t1	AT1G19180	36.207	116	70	3	96	209	49	162	3.14e-13	66.2
MS.gene25093.t1	AT1G17380	34.194	155	77	5	99	230	63	215	8.45e-13	66.2
MS.gene25093.t1	AT1G17380	31.220	205	109	7	16	202	10	200	8.59e-13	65.9
MS.gene25093.t1	AT1G74950	28.634	227	129	8	1	200	1	221	2.87e-11	61.6

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Find 48 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATGCAAAACTCAGATGG+GGG	0.692839	8.4:-72325560	MS.gene25093:CDS
AACTAAATCACTATAAGTTA+AGG	0.443726	8.4:+72323783	None:intergenic
AACTAATTTACCTGAACATT+TGG	0.278582	8.4:+72324182	None:intergenic
AATTGACGATATTCTATTCT+GGG	0.405626	8.4:-72324398	MS.gene25093:CDS
ACCTGCTTTCTTTGGGAAAA+TGG	0.109042	8.4:-72325909	MS.gene25093:CDS
ACTAGTGACCGGTGCACTTG+GGG	0.487227	8.4:+72324275	None:intergenic
AGAGGACCCTATCAAATTAA+TGG	0.329656	8.4:-72323869	MS.gene25093:CDS
AGTGTTTGTTTGAGGAATTT+CGG	0.311669	8.4:+72324236	None:intergenic
ATGATCTCAGTTGCATTCGT+AGG	0.602248	8.4:+72324348	None:intergenic
ATTCTACACCTGCTTTCTTT+GGG	0.379166	8.4:-72325916	MS.gene25093:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTGTTTGTTTGAGGAATTT+CGG	+	chr8.4:72325603-72325622	None:intergenic	30.0%
ATTGAATCTTGAATTGTTGC+AGG	-	chr8.4:72325935-72325954	MS.gene25093:CDS	30.0%
CAATTGACGATATTCTATTC+TGG	-	chr8.4:72325437-72325456	MS.gene25093:intron	30.0%
CATGACTAACCTAATTTACA+TGG	+	chr8.4:72324503-72324522	None:intergenic	30.0%
CTAACATGTATGTACGAAAA+AGG	-	chr8.4:72325176-72325195	MS.gene25093:intron	30.0%
GAATAGAATATCGTCAATTG+TGG	+	chr8.4:72325437-72325456	None:intergenic	30.0%
GAGAAAAGAAAAGAAAGGTA+TGG	-	chr8.4:72325832-72325851	MS.gene25093:intron	30.0%
GCATTAAGAAGTCTAATACA+AGG	-	chr8.4:72324355-72324374	MS.gene25093:CDS	30.0%
GGTCACTAGTGAAAAATTAA+AGG	-	chr8.4:72325574-72325593	MS.gene25093:CDS	30.0%
GTATTAGACTTCTTAATGCA+AGG	+	chr8.4:72324354-72324373	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	72323793	72326065	72323793	ID=MS.gene25093
chr8.4	mRNA	72323793	72326065	72323793	ID=MS.gene25093.t1;Parent=MS.gene25093
chr8.4	exon	72325900	72326065	72325900	ID=MS.gene25093.t1.exon1;Parent=MS.gene25093.t1
chr8.4	CDS	72325900	72326065	72325900	ID=cds.MS.gene25093.t1;Parent=MS.gene25093.t1
chr8.4	exon	72325482	72325605	72325482	ID=MS.gene25093.t1.exon2;Parent=MS.gene25093.t1
chr8.4	CDS	72325482	72325605	72325482	ID=cds.MS.gene25093.t1;Parent=MS.gene25093.t1
chr8.4	exon	72324193	72324443	72324193	ID=MS.gene25093.t1.exon3;Parent=MS.gene25093.t1
chr8.4	CDS	72324193	72324443	72324193	ID=cds.MS.gene25093.t1;Parent=MS.gene25093.t1
chr8.4	exon	72324010	72324067	72324010	ID=MS.gene25093.t1.exon4;Parent=MS.gene25093.t1
chr8.4	CDS	72324010	72324067	72324010	ID=cds.MS.gene25093.t1;Parent=MS.gene25093.t1
chr8.4	exon	72323793	72323901	72323793	ID=MS.gene25093.t1.exon5;Parent=MS.gene25093.t1
chr8.4	CDS	72323793	72323901	72323793	ID=cds.MS.gene25093.t1;Parent=MS.gene25093.t1

Gene Sequence

>MS.gene25093

ATGTCAAATTTAGCTAATACTGTGTCAGATGGCCGGAGACTCACCGGTAAGGCACCGGAGAAGTTCAAATTTTCACAGACTTGTAGTCTTTTGAGTCAGTTCTTGAAGGAAAAGAGAATTTCTGGTGATTCTACACCTGCTTTCTTTGGGAAAATGGAACCTAAAGGTATAATCTTTCTCTTGTTTCATGGTTTAATGTTTTATTTCTTACTTGGCATATGAAATTTTAATTTTAATTTTTTTGGTATTTTCTTGCCTTGTCAATTAATTGTTTGGTTTGTGATTTTTTTTCCTTATTTAATTTTATTATTATTTTGTTACAAAATGGATGATTTGGTTTAGTATTGGAAAATTATTGGGCTATTAGTAGTTGCTTAGTTAGATGAAAATGTTACATGAAGTTTATGAAATTTCCTGTGAAAGTTTGCTGATTGTGTTTTTTGGTTGTGGATTTTTCTAGCAAGTACAAAGGATTTGTTAGCCAACATGCAAAACTCAGATGGGGGTTTGAGACTAAATGCATCTGCTATTGAATCTCTTCCTCAGCTTGTGGAAAATCCTTGCATTAAGAAGTCTAATACAAGGTATGTGTGACAATTTTAATATCTGCAATTGTTATGATTTTTTTTTAATCAAGAATTGTTCTTAATTTCAAGCTAATATAGAAATTACTAAGAAATACAGTCTCCTACTATCAAAATGTTTTTCCATGTAAATTAGGTTAGTCATGGGCTGGTGAATGATGATACAATTCAGTTTTGAAAAGGTTGTGCAAGAAAAATAAACCACCACTTGTCATTAGATGTCTAGATCTATTTATAACAAAGTGGCGCAATTGGTTAATGCGCGTTAGCTTAGGTCGAAATGTTTTGGTATTACATAAATTAAATCTTGATTAAAACAATCGTCGGTCAGATTTTTTGTAAAATCGTGAATCAGTGAGAGGATCTAAACCAAAAAGAAGTATAGATCTGTTTGATTGATGTGATTTAGCAAAAGTCGAATTGTTTGGGTAGTACTTGAGTTCGATCTTGGTTGGAAAAATACTTTACTTAGTTTCTTATCAAACTGTGAATTAGTCCGAACCCAATTTCCCTTAGACTAGAAGATCAAAACGATAAATAAGTGTAGATTTGTTTGGTTCTGCGCCTTAGCTAAGGCCTAATTGTTCGGGTAGTACTCGAGTTCAATATTGGTTAGAATAATAATTGGTCAGACTTTACTTAATTTCTACTCGAACTTTGAATTAGTCGAGACTGAATTTCACTGTGGACCACAAGATCAAAATGAAAGATTTGTTTGATTGAAAAGAGGATTCTAGATTTGTTTGATCGATGAAGACCATATCATTAATACTTCAACCTTCCCAACCAAAATTGATCACTAACATGTATGTACGAAAAAGGTATTGTTGTGTTAAGTTTGGATCACCAGATCTCAACCTCACAATAAAATTAATTTACAACCAAAATAAAATGATACTCTAAACTCGTTGAACATCAATATTGTAAGAAATATTCGGTAGGATGAAAATTTTGAAAAGAAATATTTATTTGAAAAAAATATATATTGCAAGAGTTTGGTTGAACTAGTGTTCTTATTGTTGTTTATTCTTCTTGTAGATCAACAGATCCTAAAACTCCACAATTGACGATATTCTATTCTGGGAAAATGTTAGTATTTGACGCTTTTTCGCCTACGAATGCAACTGAGATCATGGAGTTGGCCACCAAATTAGCCTCTGAAAATCCAAGCGCTGAAGAAAACCCCCCAAGTGCACCGGTCACTAGTGAAAAATTAAAGGAGCCCGAAATTCCTCAAACAAACACTGCCTTAGAAACAATCGAACAAGGAAACCAAGCCAAATGTTCAGGTAAATTAGTTAATCTATCTTCATTTGTCGTTTTAACAACAAAAAAAAACATCAAATCCGAAACATTACAAAGCATGCAAGTAAAAATTCATACACTAATTTAATGTTACATTTACTTTCAACAGATATGAGATATCCAAGAAGAGCTTCACTTCTTAAATTTCTTGAGAAAAGAAAAGAAAGGTATGGACCAGTTAAAAATTTCTATTTCCGTCTGTTTATTTTATTTCCTTTGTATGTTGTCTATTTTTGTTGGTCCATGCTAATTAATTGAATCTTGAATTGTTGCAGGGTCATTGCTAGAGGACCCTATCAAATTAATGGTCACAAAAATGAGGGTAGTAGTTCTGGAAGTGAACCTAAAGATAATTCTTCCGATCAATTCGACCTTAACTTATAG

Protein sequence

>MS.gene25093.t1

MSNLANTVSDGRRLTGKAPEKFKFSQTCSLLSQFLKEKRISGDSTPAFFGKMEPKASTKDLLANMQNSDGGLRLNASAIESLPQLVENPCIKKSNTRSTDPKTPQLTIFYSGKMLVFDAFSPTNATEIMELATKLASENPSAEENPPSAPVTSEKLKEPEIPQTNTALETIEQGNQAKCSDMRYPRRASLLKFLEKRKERVIARGPYQINGHKNEGSSSGSEPKDNSSDQFDLNL