Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25341.t1 | ABN08740.1 | 80.3 | 61 | 12 | 0 | 25 | 85 | 127 | 187 | 7.60E-06 | 59.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25341.t1 | G7L3K3 | 80.3 | 61 | 12 | 0 | 25 | 85 | 127 | 187 | 5.5e-06 | 59.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene25341 | 0.80103 | 1.09E-48 | -1.69E-46 |
| MS.gene057444 | MS.gene25341 | 0.81361 | 2.35E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAGCCTAGTCCACATTTC+TGG | 0.233776 | 8.2:-38891412 | MS.gene25341:CDS |
| CTGGAACAACGTTTCGAAAC+TGG | 0.311123 | 8.2:-38891393 | None:intergenic |
| GAGATTGAAGGTGGAGAAGA+AGG | 0.384382 | 8.2:-38891590 | MS.gene25341:CDS |
| GTGGACTAAGGAACAAGAAT+TGG | 0.455229 | 8.2:-38891459 | MS.gene25341:CDS |
| AAGGTGGAGAAGAAGGGAAA+AGG | 0.458473 | 8.2:-38891583 | MS.gene25341:CDS |
| GCACGATGGTTGTGAAATAG+TGG | 0.487049 | 8.2:-38891558 | MS.gene25341:CDS |
| GGGAAGAAGGAAGAGATTGA+AGG | 0.500449 | 8.2:-38891602 | MS.gene25341:CDS |
| ATACAAAGTTTCAACTCAGA+AGG | 0.508154 | 8.2:-38891626 | MS.gene25341:CDS |
| AGGAAGAGAAAGCGCGGTGG+TGG | 0.525493 | 8.2:-38891497 | MS.gene25341:CDS |
| TACAAAGTTTCAACTCAGAA+GGG | 0.527223 | 8.2:-38891625 | MS.gene25341:CDS |
| GAAACGTTGTTCCAGAAATG+TGG | 0.531004 | 8.2:+38891401 | None:intergenic |
| GTGAAATAGTGGAAGAGTTG+AGG | 0.532067 | 8.2:-38891547 | MS.gene25341:CDS |
| AAGAAGGAAGAGATTGAAGG+TGG | 0.534159 | 8.2:-38891599 | MS.gene25341:CDS |
| AATAGTGGAAGAGTTGAGGA+AGG | 0.535399 | 8.2:-38891543 | MS.gene25341:CDS |
| AGATTGAAGGTGGAGAAGAA+GGG | 0.536092 | 8.2:-38891589 | MS.gene25341:CDS |
| ACAAAGTTTCAACTCAGAAG+GGG | 0.540390 | 8.2:-38891624 | MS.gene25341:CDS |
| TTGTTCCAGAAATGTGGACT+AGG | 0.553064 | 8.2:+38891407 | None:intergenic |
| ACAAGAATTGGCTCTGCGAA+CGG | 0.574620 | 8.2:-38891447 | MS.gene25341:CDS |
| AAGTTGAATGTGGTGTGAAA+AGG | 0.575392 | 8.2:-38891517 | MS.gene25341:CDS |
| AGTTGCACAAGGGTGGACTA+AGG | 0.587210 | 8.2:-38891471 | MS.gene25341:CDS |
| CAAAGTTTCAACTCAGAAGG+GGG | 0.603686 | 8.2:-38891623 | MS.gene25341:CDS |
| GGTGGTGGTGAAGTTGCACA+AGG | 0.634091 | 8.2:-38891482 | MS.gene25341:CDS |
| GTGGTGAAGTTGCACAAGGG+TGG | 0.656463 | 8.2:-38891478 | MS.gene25341:CDS |
| AGGAAGGAAGAAGTTGAATG+TGG | 0.671131 | 8.2:-38891527 | MS.gene25341:CDS |
| AAAGTTTCAACTCAGAAGGG+GGG | 0.695511 | 8.2:-38891622 | MS.gene25341:CDS |
| GTGGTGGTGAAGTTGCACAA+GGG | 0.698386 | 8.2:-38891481 | MS.gene25341:CDS |
| GTGAAAAGGAAGAGAAAGCG+CGG | 0.698757 | 8.2:-38891503 | MS.gene25341:CDS |
| GAAGGGAAAAGGAAGCACGA+TGG | 0.741513 | 8.2:-38891572 | MS.gene25341:CDS |
| AAAAGGAAGAGAAAGCGCGG+TGG | 0.749477 | 8.2:-38891500 | MS.gene25341:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATACAAAGTTTCAACTCAGA+AGG | - | chr8.2:38891396-38891415 | MS.gene25341:CDS | 30.0% | |
| TACAAAGTTTCAACTCAGAA+GGG | - | chr8.2:38891397-38891416 | MS.gene25341:CDS | 30.0% | |
| AAGTTGAATGTGGTGTGAAA+AGG | - | chr8.2:38891505-38891524 | MS.gene25341:CDS | 35.0% | |
| ACAAAGTTTCAACTCAGAAG+GGG | - | chr8.2:38891398-38891417 | MS.gene25341:CDS | 35.0% | |
| AAAGTTTCAACTCAGAAGGG+GGG | - | chr8.2:38891400-38891419 | MS.gene25341:CDS | 40.0% | |
| AAGAAGGAAGAGATTGAAGG+TGG | - | chr8.2:38891423-38891442 | MS.gene25341:CDS | 40.0% | |
| AGATTGAAGGTGGAGAAGAA+GGG | - | chr8.2:38891433-38891452 | MS.gene25341:CDS | 40.0% | |
| AGGAAGGAAGAAGTTGAATG+TGG | - | chr8.2:38891495-38891514 | MS.gene25341:CDS | 40.0% | |
| CAAAGTTTCAACTCAGAAGG+GGG | - | chr8.2:38891399-38891418 | MS.gene25341:CDS | 40.0% | |
| GAAACGTTGTTCCAGAAATG+TGG | + | chr8.2:38891624-38891643 | None:intergenic | 40.0% | |
| TTGTTCCAGAAATGTGGACT+AGG | + | chr8.2:38891618-38891637 | None:intergenic | 40.0% | |
| ! | AATAGTGGAAGAGTTGAGGA+AGG | - | chr8.2:38891479-38891498 | MS.gene25341:CDS | 40.0% |
| ! | GTGAAATAGTGGAAGAGTTG+AGG | - | chr8.2:38891475-38891494 | MS.gene25341:CDS | 40.0% |
| ! | GTGGACTAAGGAACAAGAAT+TGG | - | chr8.2:38891563-38891582 | MS.gene25341:CDS | 40.0% |
| AAGGTGGAGAAGAAGGGAAA+AGG | - | chr8.2:38891439-38891458 | MS.gene25341:CDS | 45.0% | |
| CAAAGCCTAGTCCACATTTC+TGG | - | chr8.2:38891610-38891629 | MS.gene25341:CDS | 45.0% | |
| GAGATTGAAGGTGGAGAAGA+AGG | - | chr8.2:38891432-38891451 | MS.gene25341:CDS | 45.0% | |
| GCACGATGGTTGTGAAATAG+TGG | - | chr8.2:38891464-38891483 | MS.gene25341:CDS | 45.0% | |
| GGGAAGAAGGAAGAGATTGA+AGG | - | chr8.2:38891420-38891439 | MS.gene25341:CDS | 45.0% | |
| GTGAAAAGGAAGAGAAAGCG+CGG | - | chr8.2:38891519-38891538 | MS.gene25341:CDS | 45.0% | |
| !! | ACAAGAATTGGCTCTGCGAA+CGG | - | chr8.2:38891575-38891594 | MS.gene25341:CDS | 45.0% |
| ! | AAAAGGAAGAGAAAGCGCGG+TGG | - | chr8.2:38891522-38891541 | MS.gene25341:CDS | 50.0% |
| ! | GAAGGGAAAAGGAAGCACGA+TGG | - | chr8.2:38891450-38891469 | MS.gene25341:CDS | 50.0% |
| ! | GTGGTGGTGAAGTTGCACAA+GGG | - | chr8.2:38891541-38891560 | MS.gene25341:CDS | 50.0% |
| !! | AGTTGCACAAGGGTGGACTA+AGG | - | chr8.2:38891551-38891570 | MS.gene25341:CDS | 50.0% |
| CAACTCAGAAGGGGGGAAGA+AGG | - | chr8.2:38891407-38891426 | MS.gene25341:CDS | 55.0% | |
| GTGGTGAAGTTGCACAAGGG+TGG | - | chr8.2:38891544-38891563 | MS.gene25341:CDS | 55.0% | |
| ! | GGTGGTGGTGAAGTTGCACA+AGG | - | chr8.2:38891540-38891559 | MS.gene25341:CDS | 55.0% |
| ! | AGGAAGAGAAAGCGCGGTGG+TGG | - | chr8.2:38891525-38891544 | MS.gene25341:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 38891394 | 38891650 | 38891394 | ID=MS.gene25341 |
| chr8.2 | mRNA | 38891394 | 38891650 | 38891394 | ID=MS.gene25341.t1;Parent=MS.gene25341 |
| chr8.2 | exon | 38891394 | 38891650 | 38891394 | ID=MS.gene25341.t1.exon1;Parent=MS.gene25341.t1 |
| chr8.2 | CDS | 38891394 | 38891650 | 38891394 | ID=cds.MS.gene25341.t1;Parent=MS.gene25341.t1 |
| Gene Sequence |
| Protein sequence |
