Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25777.t1 | PNX59699.1 | 55.4 | 56 | 19 | 1 | 29 | 78 | 30 | 85 | 9.30E-07 | 63.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25777.t1 | A0A2K3K079 | 55.4 | 56 | 19 | 1 | 29 | 78 | 30 | 85 | 6.7e-07 | 63.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054801 | MS.gene25777 | 0.811025 | 8.61E-51 | -1.69E-46 |
| MS.gene055357 | MS.gene25777 | 0.801583 | 8.41E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene25777 | 0.809442 | 1.89E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene25777 | 0.81037 | 1.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25777.t1 | MTR_3g080890 | 44.660 | 103 | 24 | 3 | 1 | 88 | 1 | 85 | 4.45e-12 | 58.9 |
| MS.gene25777.t1 | MTR_1g021965 | 63.415 | 41 | 15 | 0 | 23 | 63 | 28 | 68 | 2.76e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGCCAACGACGGAAATTA+TGG | 0.246928 | 3.2:+70851306 | None:intergenic |
| TGCTCTCGAGTGCAAAGCTT+TGG | 0.369375 | 3.2:+70850800 | None:intergenic |
| ACTTCAATTCTGGAAACGTC+TGG | 0.392283 | 3.2:+70851338 | None:intergenic |
| TTGCTCTGAGTGCAAAGCTT+TGG | 0.420353 | 3.2:+70850893 | None:intergenic |
| AGCTCTGCATTCCATTCTGC+TGG | 0.426420 | 3.2:+70850923 | None:intergenic |
| AAGGCCATAATTTCCGTCGT+TGG | 0.491561 | 3.2:-70851310 | MS.gene25777:CDS |
| AGCTCCGCATTCCATTCTGC+TGG | 0.500843 | 3.2:+70850830 | None:intergenic |
| CATTGAAGACACCAGCAGAA+TGG | 0.516038 | 3.2:-70850934 | MS.gene25777:intron |
| ACAATCTAAGGAATTATGGC+TGG | 0.522622 | 3.2:-70851461 | None:intergenic |
| CTTCACACTGCTAGACATCC+AGG | 0.527754 | 3.2:-70851276 | MS.gene25777:intron |
| CATTGAAGACACCAGCAGAA+TGG | 0.535892 | 3.2:-70850841 | MS.gene25777:CDS |
| CTTCAATTCTGGAAACGTCT+GGG | 0.550347 | 3.2:+70851339 | None:intergenic |
| TACTGATCCTCCTCCGACAG+TGG | 0.555859 | 3.2:-70851381 | MS.gene25777:CDS |
| TAGGTGACCACTGTCGGAGG+AGG | 0.579910 | 3.2:+70851374 | None:intergenic |
| TTTGCATAGGTGACCACTGT+CGG | 0.584337 | 3.2:+70851368 | None:intergenic |
| GAGGATCAGTAGCAACAACA+AGG | 0.606584 | 3.2:+70851393 | None:intergenic |
| TGACAATAAGAAGAATGTCT+CGG | 0.627026 | 3.2:-70851423 | MS.gene25777:CDS |
| AGATGCGTTCATGCCAACGA+CGG | 0.660184 | 3.2:+70851297 | None:intergenic |
| GCATAGGTGACCACTGTCGG+AGG | 0.668853 | 3.2:+70851371 | None:intergenic |
| AGGATCAGTAGCAACAACAA+GGG | 0.691715 | 3.2:+70851394 | None:intergenic |
| GACACCAGCAGAATGGAATG+CGG | 0.699910 | 3.2:-70850834 | MS.gene25777:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTATTGCTTGTATGAGTC+AGG | + | chr3.2:70851234-70851253 | None:intergenic | 30.0% | |
| TACGTTATCTGATTTACGTT+TGG | - | chr3.2:70851008-70851027 | MS.gene25777:intron | 30.0% | |
| TGACAATAAGAAGAATGTCT+CGG | - | chr3.2:70850816-70850835 | MS.gene25777:CDS | 30.0% | |
| ! | ATAACGTAGAAAGAAGTGTA+TGG | + | chr3.2:70850996-70851015 | None:intergenic | 30.0% |
| ! | TCCAGAATTGAAGTTTTTGA+AGG | - | chr3.2:70850910-70850929 | MS.gene25777:CDS | 30.0% |
| !! | ACAACATGAAAGTTTGTTGT+TGG | - | chr3.2:70851070-70851089 | MS.gene25777:intron | 30.0% |
| AATTTCTTTGGCAACCTTGT+AGG | + | chr3.2:70851046-70851065 | None:intergenic | 35.0% | |
| ACAAGGTTGCCAAAGAAATT+TGG | - | chr3.2:70851046-70851065 | MS.gene25777:intron | 35.0% | |
| CAAGGTTGCCAAAGAAATTT+GGG | - | chr3.2:70851047-70851066 | MS.gene25777:intron | 35.0% | |
| CATGTTGTCCCAAATTTCTT+TGG | + | chr3.2:70851058-70851077 | None:intergenic | 35.0% | |
| CCTTCAATTCTTAAAGGAGT+TGG | + | chr3.2:70851155-70851174 | None:intergenic | 35.0% | |
| GCCTTCAAAAACTTCAATTC+TGG | + | chr3.2:70850914-70850933 | None:intergenic | 35.0% | |
| TCTGTGATGACAAATGAAAG+AGG | - | chr3.2:70851099-70851118 | MS.gene25777:intron | 35.0% | |
| TGTCTCTTAATGATCTCATC+AGG | - | chr3.2:70851274-70851293 | MS.gene25777:intron | 35.0% | |
| ! | ACTCCTTTAAGAATTGAAGG+CGG | - | chr3.2:70851155-70851174 | MS.gene25777:intron | 35.0% |
| ! | CCAACTCCTTTAAGAATTGA+AGG | - | chr3.2:70851152-70851171 | MS.gene25777:intron | 35.0% |
| AGGATCAGTAGCAACAACAA+GGG | + | chr3.2:70850848-70850867 | None:intergenic | 40.0% | |
| GGATGTTTACTGTTCCTACA+AGG | - | chr3.2:70851029-70851048 | MS.gene25777:intron | 40.0% | |
| TCTCCGCCTTCAATTCTTAA+AGG | + | chr3.2:70851161-70851180 | None:intergenic | 40.0% | |
| ! | ACTTCAATTCTGGAAACGTC+TGG | + | chr3.2:70850904-70850923 | None:intergenic | 40.0% |
| ! | CTTCAATTCTGGAAACGTCT+GGG | + | chr3.2:70850903-70850922 | None:intergenic | 40.0% |
| AAGGCCATAATTTCCGTCGT+TGG | - | chr3.2:70850929-70850948 | MS.gene25777:CDS | 45.0% | |
| CATGCCAACGACGGAAATTA+TGG | + | chr3.2:70850936-70850955 | None:intergenic | 45.0% | |
| CATTGAAGACACCAGCAGAA+TGG | - | chr3.2:70851305-70851324 | MS.gene25777:CDS | 45.0% | |
| GAGGATCAGTAGCAACAACA+AGG | + | chr3.2:70850849-70850868 | None:intergenic | 45.0% | |
| TTTGCATAGGTGACCACTGT+CGG | + | chr3.2:70850874-70850893 | None:intergenic | 45.0% | |
| ! | GTCAGGCAACTTTTCAACCA+AGG | + | chr3.2:70851217-70851236 | None:intergenic | 45.0% |
| ! | GTCTGGGAACTGTTTTGCAT+AGG | + | chr3.2:70850887-70850906 | None:intergenic | 45.0% |
| ! | TTGCTCTGAGTGCAAAGCTT+TGG | + | chr3.2:70851349-70851368 | None:intergenic | 45.0% |
| AGATGCGTTCATGCCAACGA+CGG | + | chr3.2:70850945-70850964 | None:intergenic | 50.0% | |
| AGCTCTGCATTCCATTCTGC+TGG | + | chr3.2:70851319-70851338 | None:intergenic | 50.0% | |
| CTTCACACTGCTAGACATCC+AGG | - | chr3.2:70850963-70850982 | MS.gene25777:intron | 50.0% | |
| CTTTCACACGTGTTTGTCGC+TGG | - | chr3.2:70851189-70851208 | MS.gene25777:intron | 50.0% | |
| GAAGTGTATGGCGACAAACC+TGG | + | chr3.2:70850984-70851003 | None:intergenic | 50.0% | |
| GACACCAGCAGAATGGAATG+CGG | - | chr3.2:70851312-70851331 | MS.gene25777:CDS | 50.0% | |
| TGCTCTCGAGTGCAAAGCTT+TGG | + | chr3.2:70851442-70851461 | None:intergenic | 50.0% | |
| AGCTCCGCATTCCATTCTGC+TGG | + | chr3.2:70851412-70851431 | None:intergenic | 55.0% | |
| TACTGATCCTCCTCCGACAG+TGG | - | chr3.2:70850858-70850877 | MS.gene25777:CDS | 55.0% | |
| CGTGTTTGTCGCTGGTGCCT+TGG | - | chr3.2:70851197-70851216 | MS.gene25777:intron | 60.0% | |
| GCATAGGTGACCACTGTCGG+AGG | + | chr3.2:70850871-70850890 | None:intergenic | 60.0% | |
| TAGGTGACCACTGTCGGAGG+AGG | + | chr3.2:70850868-70850887 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 70850793 | 70851468 | 70850793 | ID=MS.gene25777 |
| chr3.2 | mRNA | 70850793 | 70851468 | 70850793 | ID=MS.gene25777.t1;Parent=MS.gene25777 |
| chr3.2 | exon | 70851277 | 70851468 | 70851277 | ID=MS.gene25777.t1.exon1;Parent=MS.gene25777.t1 |
| chr3.2 | CDS | 70851277 | 70851468 | 70851277 | ID=cds.MS.gene25777.t1;Parent=MS.gene25777.t1 |
| chr3.2 | exon | 70850793 | 70850948 | 70850793 | ID=MS.gene25777.t1.exon2;Parent=MS.gene25777.t1 |
| chr3.2 | CDS | 70850793 | 70850948 | 70850793 | ID=cds.MS.gene25777.t1;Parent=MS.gene25777.t1 |
| Gene Sequence |
| Protein sequence |
