AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26573

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26573.t1 MTR_4g087420 97.940 534 11 0 1 534 1 534 0.0 1065
MS.gene26573.t1 MTR_6g033415 36.522 345 176 13 140 474 101 412 2.41e-39 149
MS.gene26573.t1 MTR_7g100790 34.551 356 182 11 146 474 108 439 5.31e-38 145
MS.gene26573.t1 MTR_1g066800 31.921 354 187 10 144 474 114 436 2.65e-35 138
MS.gene26573.t1 MTR_1g066800 31.921 354 187 10 144 474 119 441 3.46e-35 138
MS.gene26573.t1 MTR_0047s0040 26.276 529 276 19 3 522 4 427 3.67e-33 131
MS.gene26573.t1 MTR_1g095740 37.297 185 107 4 144 327 139 315 1.53e-31 127
MS.gene26573.t1 MTR_8g024540 27.619 525 287 17 1 472 1 485 1.61e-31 128
MS.gene26573.t1 MTR_7g114700 28.824 340 179 10 142 476 131 412 1.74e-28 118
MS.gene26573.t1 MTR_4g050480 33.724 341 170 17 148 479 99 392 3.13e-23 102
MS.gene26573.t1 MTR_4g050480 33.724 341 170 17 148 479 98 391 3.56e-23 102
MS.gene26573.t1 MTR_2g078210 32.432 185 105 5 137 316 65 234 3.88e-21 94.7
MS.gene26573.t1 MTR_2g078210 32.432 185 105 5 137 316 5 174 5.01e-21 93.2
MS.gene26573.t1 MTR_2g015370 32.749 171 101 5 147 316 64 221 2.30e-19 89.0
MS.gene26573.t1 MTR_3g104570 27.869 366 199 12 111 445 55 386 3.45e-19 90.5
MS.gene26573.t1 MTR_1g007920 30.938 320 156 10 148 442 99 378 3.04e-17 84.7
MS.gene26573.t1 MTR_1g007920 30.938 320 156 10 148 442 124 403 6.12e-17 84.0
MS.gene26573.t1 MTR_0019s0070 43.038 79 45 0 142 220 113 191 2.42e-16 82.0
MS.gene26573.t1 MTR_3g020990 32.624 141 81 2 159 299 103 229 3.53e-16 78.6
MS.gene26573.t1 MTR_3g091560 26.899 316 165 8 148 454 114 372 1.48e-14 76.3
MS.gene26573.t1 MTR_1g101330 44.776 67 37 0 145 211 114 180 2.48e-14 75.5
MS.gene26573.t1 MTR_8g102400 41.667 84 43 1 144 221 110 193 1.56e-13 73.2
MS.gene26573.t1 MTR_1g051810 31.206 141 86 3 147 282 106 240 2.70e-13 72.0
MS.gene26573.t1 MTR_8g074730 50.000 62 31 0 148 209 73 134 2.84e-13 67.8
MS.gene26573.t1 MTR_7g114870 46.970 66 35 0 148 213 108 173 4.11e-13 71.6
MS.gene26573.t1 MTR_4g108140 48.387 62 32 0 148 209 101 162 4.33e-13 72.4
MS.gene26573.t1 MTR_4g108140 48.387 62 32 0 148 209 102 163 4.42e-13 72.0
MS.gene26573.t1 MTR_2g100740 51.613 62 30 0 147 208 98 159 9.20e-13 70.1
MS.gene26573.t1 MTR_4g102840 50.820 61 30 0 148 208 90 150 9.88e-12 67.4
MS.gene26573.t1 MTR_8g014580 45.902 61 33 0 148 208 128 188 1.21e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26573.t1 AT3G59690 51.232 568 190 18 1 533 1 516 5.62e-160 466
MS.gene26573.t1 AT3G59690 51.232 568 190 18 1 533 1 516 5.62e-160 466
MS.gene26573.t1 AT2G43680 52.550 451 140 16 92 534 285 669 2.14e-123 377
MS.gene26573.t1 AT2G43680 55.396 139 45 5 1 126 1 135 1.69e-25 111
MS.gene26573.t1 AT2G43680 52.550 451 140 16 92 534 284 668 2.44e-123 377
MS.gene26573.t1 AT2G43680 58.696 138 41 5 1 126 1 134 2.15e-25 110
MS.gene26573.t1 AT2G43680 52.550 451 140 16 92 534 284 668 2.44e-123 377
MS.gene26573.t1 AT2G43680 58.696 138 41 5 1 126 1 134 2.15e-25 110
MS.gene26573.t1 AT2G43680 52.550 451 140 16 92 534 284 668 2.44e-123 377
MS.gene26573.t1 AT2G43680 58.696 138 41 5 1 126 1 134 2.15e-25 110
MS.gene26573.t1 AT2G43680 52.550 451 140 16 92 534 284 668 2.44e-123 377
MS.gene26573.t1 AT2G43680 58.696 138 41 5 1 126 1 134 2.15e-25 110
MS.gene26573.t1 AT5G03040 30.242 496 267 18 1 477 1 436 4.81e-41 154
MS.gene26573.t1 AT5G03040 30.242 496 267 18 1 477 1 436 4.81e-41 154
MS.gene26573.t1 AT5G03040 30.242 496 267 18 1 477 1 436 4.81e-41 154
MS.gene26573.t1 AT3G52290 34.202 307 168 10 145 444 107 386 1.25e-39 149
MS.gene26573.t1 AT1G01110 28.081 495 262 20 1 449 1 447 7.61e-31 126
MS.gene26573.t1 AT5G13460 29.819 332 163 10 118 449 90 351 8.07e-29 119
MS.gene26573.t1 AT2G26410 36.898 187 98 6 145 325 147 319 6.16e-27 114
MS.gene26573.t1 AT2G26410 36.898 187 98 6 145 325 147 319 6.16e-27 114
MS.gene26573.t1 AT3G49260 29.139 302 175 6 148 441 122 392 2.83e-26 112
MS.gene26573.t1 AT3G49260 29.139 302 175 6 148 441 122 392 2.83e-26 112
MS.gene26573.t1 AT3G49260 29.139 302 175 6 148 441 122 392 2.83e-26 112
MS.gene26573.t1 AT3G49260 28.146 302 179 5 148 441 122 393 4.31e-26 111
MS.gene26573.t1 AT3G09710 38.596 171 94 4 145 314 107 267 3.42e-25 108
MS.gene26573.t1 AT4G00820 31.044 364 195 15 145 473 131 473 1.18e-24 108
MS.gene26573.t1 AT3G09710 37.079 178 101 4 145 314 107 281 3.79e-24 105
MS.gene26573.t1 AT2G26180 34.804 204 113 6 148 341 86 279 4.42e-23 102
MS.gene26573.t1 AT2G33990 33.032 221 119 9 148 363 49 245 3.52e-22 96.7
MS.gene26573.t1 AT3G22190 38.068 176 85 5 148 315 90 249 9.07e-22 98.2
MS.gene26573.t1 AT3G22190 38.068 176 85 5 148 315 90 249 9.07e-22 98.2
MS.gene26573.t1 AT3G22190 38.068 176 85 5 148 315 90 249 9.07e-22 98.2
MS.gene26573.t1 AT3G22190 38.068 176 85 5 148 315 90 249 9.07e-22 98.2
MS.gene26573.t1 AT3G15050 32.735 223 127 10 131 343 27 236 2.17e-18 85.5
MS.gene26573.t1 AT1G17480 36.296 135 67 2 148 282 96 211 5.06e-18 86.3
MS.gene26573.t1 AT4G10640 33.333 165 83 4 140 283 94 252 7.88e-18 86.3
MS.gene26573.t1 AT1G72670 36.879 141 79 3 142 282 89 219 2.30e-17 84.7
MS.gene26573.t1 AT5G03960 35.036 137 84 1 146 282 107 238 1.52e-14 76.3
MS.gene26573.t1 AT5G03960 35.036 137 84 1 146 282 108 239 1.54e-14 76.3
MS.gene26573.t1 AT5G03960 35.036 137 84 1 146 282 108 239 1.54e-14 76.3
MS.gene26573.t1 AT4G14750 45.588 68 37 0 145 212 104 171 6.49e-14 73.9
MS.gene26573.t1 AT4G14750 47.692 65 34 0 148 212 166 230 1.03e-13 73.9
MS.gene26573.t1 AT4G14750 45.588 68 37 0 145 212 163 230 1.05e-13 73.9
MS.gene26573.t1 AT1G18840 44.615 65 36 0 145 209 108 172 1.41e-13 73.9
MS.gene26573.t1 AT1G18840 44.615 65 36 0 145 209 108 172 1.41e-13 73.9
MS.gene26573.t1 AT1G18840 44.615 65 36 0 145 209 108 172 1.41e-13 73.9
MS.gene26573.t1 AT5G62070 51.786 56 27 0 145 200 116 171 2.49e-13 72.4
MS.gene26573.t1 AT3G16490 48.387 62 32 0 148 209 109 170 2.87e-13 72.0
MS.gene26573.t1 AT5G07240 46.774 62 33 0 145 206 105 166 3.82e-13 71.6
MS.gene26573.t1 AT5G07240 46.774 62 33 0 145 206 151 212 4.61e-13 71.6
MS.gene26573.t1 AT3G49380 37.634 93 55 1 145 234 109 201 1.32e-12 69.7
MS.gene26573.t1 AT1G74690 43.939 66 37 0 145 210 112 177 3.02e-12 69.7
MS.gene26573.t1 AT3G51380 42.857 70 40 0 140 209 31 100 3.23e-12 63.5
MS.gene26573.t1 AT1G51960 42.857 77 39 1 148 224 102 173 4.40e-12 68.2
MS.gene26573.t1 AT4G29150 43.750 64 36 0 145 208 130 193 6.72e-12 67.8
MS.gene26573.t1 AT1G01110 25.085 295 172 12 191 449 3 284 4.23e-11 65.1

Find 150 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGCATTGAGAAAATATT+TGG 0.147656 4.1:-22756234 MS.gene26573:CDS
ATAGTCGATCTTGTTGATTT+TGG 0.221689 4.1:+22754571 None:intergenic
AATCACACAAATCTTGTAAA+AGG 0.233035 4.1:+22754073 None:intergenic
GGCTTTCTTTCAACCCTTGC+AGG 0.240511 4.1:+22754094 None:intergenic
GTGTGAATTGGAAATTCTTC+AGG 0.281430 4.1:+22754686 None:intergenic
ACAGGCGTTTGAGTTGATTT+TGG 0.283729 4.1:+22754796 None:intergenic
TTTCCTTGTGGAGTTCTAAA+TGG 0.286373 4.1:+22754517 None:intergenic
TCAAGTGACTGGTATTTATC+TGG 0.291478 4.1:+22754157 None:intergenic
GTCAATGTTCTCTAGAAATA+AGG 0.300536 4.1:-22754203 MS.gene26573:CDS
TTTCCTTTGTCACAAGGAAT+AGG 0.310206 4.1:-22754247 MS.gene26573:CDS
GGGTCATTCAAATGGAATAA+AGG 0.327034 4.1:-22754226 MS.gene26573:CDS
CTCTTGGAATCAGTGCTTGT+TGG 0.334476 4.1:+22754280 None:intergenic
TGAGAGCTTGAGCCAGGTCT+TGG 0.341364 4.1:+22754631 None:intergenic
TACCAGTCACTTGAATCTAT+TGG 0.350119 4.1:-22754148 MS.gene26573:CDS
ACAGTTGGAGCCATGTAGTT+TGG 0.351130 4.1:+22754379 None:intergenic
GGCTGCTTACAGAGGTTATA+TGG 0.351906 4.1:-22755938 MS.gene26573:intron
CACACCACCAAGACCATATT+CGG 0.361031 4.1:-22754668 MS.gene26573:CDS
TTCCTTTGTCACAAGGAATA+GGG 0.361875 4.1:-22754246 MS.gene26573:CDS
CAGAGCTCTAAAACTCTTCC+TGG 0.366223 4.1:+22755621 None:intergenic
ACCTTTCGACGTACCATTGA+AGG 0.378126 4.1:-22754440 MS.gene26573:CDS
GCAGCATTGAGAAAATATTT+GGG 0.385983 4.1:-22756233 MS.gene26573:CDS
TGGTTCTGTAAAGTTGGTTC+TGG 0.389882 4.1:+22755985 None:intergenic
TAACAGCTGCATGTGTTTCA+TGG 0.394012 4.1:+22755534 None:intergenic
TTCCGCGAAGATGCTGCTTT+CGG 0.397333 4.1:+22756039 None:intergenic
ATGGTCTTGGTGGTGTGAAT+TGG 0.398616 4.1:+22754674 None:intergenic
GGTCATTCAAATGGAATAAA+GGG 0.400507 4.1:-22754225 MS.gene26573:CDS
CTAAATGGAGGAGTTCTAGA+TGG 0.403634 4.1:+22754532 None:intergenic
GGCCTTTGAGAGCTTGAGCC+AGG 0.404316 4.1:+22754625 None:intergenic
AAAGGTTTGGTAAGGCTTCA+AGG 0.407441 4.1:-22755598 MS.gene26573:CDS
AATGAAGCTGGAAGTACCTT+GGG 0.409619 4.1:-22755433 MS.gene26573:CDS
TGCTGCTGAGATGGGAAAGA+AGG 0.411748 4.1:-22757201 None:intergenic
AACAACAAGTACTTGCAATT+AGG 0.413110 4.1:-22756197 MS.gene26573:CDS
TGGTACGTCGAAAGGTGAAT+TGG 0.417209 4.1:+22754447 None:intergenic
CCACCGCCAAATCTTTCTCT+TGG 0.423496 4.1:+22754322 None:intergenic
ACCTTGGGGAAAAGTGCTTT+AGG 0.429209 4.1:-22755418 MS.gene26573:CDS
AGGCAGGTAACAATGAAGAT+TGG 0.438407 4.1:-22755223 MS.gene26573:CDS
AGTAATCCAAGAGAAAGATT+TGG 0.440973 4.1:-22754328 MS.gene26573:CDS
TTTGGTCGCTCGGGAGGTGT+GGG 0.442251 4.1:+22756171 None:intergenic
AAGGAATAGGGTCATTCAAA+TGG 0.447925 4.1:-22754234 MS.gene26573:CDS
GCCCTTGGAGATGGATCCCT+TGG 0.448654 4.1:+22756084 None:intergenic
ACTCGGCTTGATATCGTGCC+TGG 0.450049 4.1:+22755461 None:intergenic
GGCATTTGCATATTCACATC+AGG 0.465718 4.1:-22755117 MS.gene26573:intron
GGCAGGTAACAATGAAGATT+GGG 0.469502 4.1:-22755222 MS.gene26573:CDS
CTCCGAAAGCAGCATCTTCG+CGG 0.469709 4.1:-22756041 MS.gene26573:CDS
GATTCCCATGGTGGTGGAGC+TGG 0.474191 4.1:-22754753 MS.gene26573:CDS
GGCTTAGGAGAAGGAGGCCT+AGG 0.481446 4.1:+22756114 None:intergenic
TCCAGCTTCATTCTTGTACT+CGG 0.482184 4.1:+22755444 None:intergenic
AGGAAACGAGACTCTCCTCT+TGG 0.484468 4.1:+22754264 None:intergenic
TCCTTCAATGGTACGTCGAA+AGG 0.487272 4.1:+22754439 None:intergenic
GAGCTCTGAAAGGTTTGGTA+AGG 0.487495 4.1:-22755606 MS.gene26573:CDS
GCGTGACGTCAAAATATTCA+AGG 0.487546 4.1:-22754486 MS.gene26573:CDS
GGAGAAGCGGCCCTTGGAGA+TGG 0.488016 4.1:+22756075 None:intergenic
AGATGGGAAAGAAGGGGAGT+TGG 0.488180 4.1:-22757193 MS.gene26573:CDS
TGCTGCTTGGTTCTCTGAAG+AGG 0.488717 4.1:+22756250 None:intergenic
TCTTTCAACCCTTGCAGGCA+AGG 0.489990 4.1:+22754099 None:intergenic
TCCCTTGGAGAAGGAGGCTT+AGG 0.492264 4.1:+22756099 None:intergenic
TGTCTCGGCGACTAAGATCC+AGG 0.493274 4.1:-22755959 MS.gene26573:CDS
CTCCTAAGCCTCCTTCTCCA+AGG 0.498883 4.1:-22756101 MS.gene26573:CDS
GCCGAGTACAAGAATGAAGC+TGG 0.501793 4.1:-22755445 MS.gene26573:CDS
CTCCTAGAGTTGCTTCTCCT+AGG 0.502361 4.1:-22756131 MS.gene26573:CDS
ACATGCTGGTTCTGTAAAGT+TGG 0.510843 4.1:+22755979 None:intergenic
ACTCGCGGAGAAGCGGCCCT+TGG 0.513311 4.1:+22756069 None:intergenic
GAATGAAGCTGGAAGTACCT+TGG 0.515373 4.1:-22755434 MS.gene26573:CDS
AGGCTTCAAGGAGTGGTAAG+AGG 0.518282 4.1:-22755586 MS.gene26573:CDS
GGTCGCTCGGGAGGTGTGGG+AGG 0.522529 4.1:+22756174 None:intergenic
TGTGAATTGGAAATTCTTCA+GGG 0.522775 4.1:+22754687 None:intergenic
AGACTGTCATCGTCCTTCAA+TGG 0.522835 4.1:+22754427 None:intergenic
TATTCAAGGCCAAGGGGAGT+AGG 0.527231 4.1:-22754472 MS.gene26573:CDS
AGCACAAAAGAGAAGAAGAA+AGG 0.528405 4.1:-22756315 MS.gene26573:CDS
GAATTGGATCCTACTCCCCT+TGG 0.530551 4.1:+22754463 None:intergenic
CTCGATCGCGTATCTGTCAC+AGG 0.530625 4.1:+22754778 None:intergenic
GACCCTATTCCTTGTGACAA+AGG 0.531331 4.1:+22754244 None:intergenic
CGAGCGGATTCCCATGGTGG+TGG 0.531759 4.1:-22754759 MS.gene26573:CDS
TACACATCACTCTAAGGGAA+AGG 0.537172 4.1:-22757147 MS.gene26573:intron
CGTCAAAATATTCAAGGCCA+AGG 0.538050 4.1:-22754480 MS.gene26573:CDS
TTCCAATAGATTCAAGTGAC+TGG 0.538161 4.1:+22754146 None:intergenic
GCTGCTGAGATGGGAAAGAA+GGG 0.540744 4.1:-22757200 None:intergenic
TCCTAAGCCTCCTTCTCCAA+GGG 0.542552 4.1:-22756100 MS.gene26573:CDS
AGCAGTATCCTTGCCTGCAA+GGG 0.545645 4.1:-22754107 MS.gene26573:CDS
ATTACTACTAGCGCGTACTT+TGG 0.546568 4.1:+22754345 None:intergenic
GCTGCTTGGTTCTCTGAAGA+GGG 0.546752 4.1:+22756251 None:intergenic
AGAAGCGAGGTTGCAGAGGA+AGG 0.547317 4.1:-22755171 MS.gene26573:CDS
TCCATGTTATCAAAGGCAAA+AGG 0.548096 4.1:+22754604 None:intergenic
TCTTAGTCGCCGAGACATGC+TGG 0.550419 4.1:+22755965 None:intergenic
TTGGCCTTGGCAGAAACAGT+TGG 0.550995 4.1:+22754364 None:intergenic
TGATAGCTTACTGACAAAAG+AGG 0.552866 4.1:-22755198 MS.gene26573:CDS
ACGCTTGAAGTGTTTCCTTG+TGG 0.554504 4.1:+22754505 None:intergenic
AAGTAGAAGCGAGGTTGCAG+AGG 0.554875 4.1:-22755175 MS.gene26573:CDS
GTCTCGTTTCCTTTGTCACA+AGG 0.555248 4.1:-22754253 MS.gene26573:CDS
CAGCAGTATCCTTGCCTGCA+AGG 0.555494 4.1:-22754108 MS.gene26573:CDS
GATGGATCCCTTGGAGAAGG+AGG 0.557622 4.1:+22756093 None:intergenic
AATCCAGATGTTAGAAAACC+AGG 0.559159 4.1:-22755479 MS.gene26573:CDS
ACTAGCGCGTACTTTGGCCT+TGG 0.561095 4.1:+22754351 None:intergenic
AGGATTCTCATGGCTCTCAC+AGG 0.561873 4.1:-22754183 MS.gene26573:CDS
TGTTCCGAATATGGTCTTGG+TGG 0.562203 4.1:+22754664 None:intergenic
CTAGGAGAAGCAACTCTAGG+AGG 0.564157 4.1:+22756132 None:intergenic
TATAACCTCTGTAAGCAGCC+TGG 0.564353 4.1:+22755941 None:intergenic
AAAGAGAAGAAGAAAGGTGT+AGG 0.565399 4.1:-22756309 MS.gene26573:CDS
GGCTCCAACTGTTTCTGCCA+AGG 0.565988 4.1:-22754368 MS.gene26573:CDS
CATCAAAAGAGAGAGATCAA+TGG 0.567226 4.1:-22755138 MS.gene26573:CDS
CCTTGTGGAGTTCTAAATGG+AGG 0.567299 4.1:+22754520 None:intergenic
AGATTTGGCGGTGGTAGTAG+TGG 0.568746 4.1:-22754313 MS.gene26573:CDS
ATGTAGTTTGGAACGGAGAA+CGG 0.569098 4.1:+22754391 None:intergenic
GGGAAAAGTGCTTTAGGACA+TGG 0.569234 4.1:-22755412 MS.gene26573:CDS
CCAACCAGCTCCACCACCAT+GGG 0.569406 4.1:+22754749 None:intergenic
GTCAAAATATTCAAGGCCAA+GGG 0.570622 4.1:-22754479 MS.gene26573:CDS
TTTGGTAAGGCTTCAAGGAG+TGG 0.571343 4.1:-22755593 MS.gene26573:CDS
AATCCAAGAGAAAGATTTGG+CGG 0.577080 4.1:-22754325 MS.gene26573:CDS
GGAGATGGATCCCTTGGAGA+AGG 0.577659 4.1:+22756090 None:intergenic
TGCTTGGTTCTCTGAAGAGG+GGG 0.580393 4.1:+22756253 None:intergenic
CTCCAAGGGATCCATCTCCA+AGG 0.580760 4.1:-22756086 MS.gene26573:CDS
GGTGTAGGAAAACTAAAGCA+TGG 0.583060 4.1:-22756294 MS.gene26573:CDS
AGAGGTAACTCGCGGAGAAG+CGG 0.585392 4.1:+22756062 None:intergenic
TGGTGTATCCATGTTATCAA+AGG 0.590388 4.1:+22754597 None:intergenic
GGAGAAGGAGGCTTAGGAGA+AGG 0.593364 4.1:+22756105 None:intergenic
TGGAGCCATGTAGTTTGGAA+CGG 0.593443 4.1:+22754384 None:intergenic
GGCCTAGGAGAAGCAACTCT+AGG 0.595533 4.1:+22756129 None:intergenic
GACCTGGCTCAAGCTCTCAA+AGG 0.598818 4.1:-22754627 MS.gene26573:CDS
TCCAAGGGATCCATCTCCAA+GGG 0.601031 4.1:-22756085 MS.gene26573:CDS
TAGGAGAAGCAACTCTAGGA+GGG 0.605104 4.1:+22756133 None:intergenic
CCTCCATTTAGAACTCCACA+AGG 0.606509 4.1:-22754520 MS.gene26573:CDS
GAAGGAGGCTTAGGAGAAGG+AGG 0.609274 4.1:+22756108 None:intergenic
TTTACAGAACCAGCATGTCT+CGG 0.610936 4.1:-22755974 MS.gene26573:CDS
TCTAGAAATAAGGATTCTCA+TGG 0.612036 4.1:-22754193 MS.gene26573:CDS
TCCAACCAGCTCCACCACCA+TGG 0.613063 4.1:+22754748 None:intergenic
GTTCTCCGTTCCAAACTACA+TGG 0.616839 4.1:-22754389 MS.gene26573:CDS
CGCGATCGAGCGGATTCCCA+TGG 0.617077 4.1:-22754765 MS.gene26573:CDS
ATCTATTGGAAATGTGAGTG+TGG 0.620035 4.1:-22754134 MS.gene26573:CDS
ACTCTAGGAGGGACGAAAGG+TGG 0.620082 4.1:+22756144 None:intergenic
ACTATGTTAACAGTTATGGA+AGG 0.620863 4.1:-22754824 MS.gene26573:intron
CCAAGAGAAAGATTTGGCGG+TGG 0.631411 4.1:-22754322 MS.gene26573:CDS
CAAAAGACAAGTCCAAGACC+TGG 0.632412 4.1:-22754643 MS.gene26573:CDS
CAGCATTGAGAAAATATTTG+GGG 0.632868 4.1:-22756232 MS.gene26573:CDS
GATCGAGCGGATTCCCATGG+TGG 0.638826 4.1:-22754762 MS.gene26573:CDS
CTGCTTGGTTCTCTGAAGAG+GGG 0.639910 4.1:+22756252 None:intergenic
GCAACTCTAGGAGGGACGAA+AGG 0.644669 4.1:+22756141 None:intergenic
AAGATCCAGGCTGCTTACAG+AGG 0.646831 4.1:-22755946 MS.gene26573:CDS
CCCATGGTGGTGGAGCTGGT+TGG 0.648003 4.1:-22754749 MS.gene26573:CDS
TAAGAGGACAGAATGTGAAG+AGG 0.651089 4.1:-22755570 MS.gene26573:CDS
TCAAAATATTCAAGGCCAAG+GGG 0.659140 4.1:-22754478 MS.gene26573:CDS
CAACAAGCACTGATTCCAAG+AGG 0.666446 4.1:-22754279 MS.gene26573:CDS
GTGACAGATACGCGATCGAG+CGG 0.669089 4.1:-22754775 MS.gene26573:CDS
CGAAGATGCTGCTTTCGGAG+AGG 0.691044 4.1:+22756044 None:intergenic
ATGAAGCTGGAAGTACCTTG+GGG 0.697268 4.1:-22755432 MS.gene26573:CDS
GCGGAATGTTCATCAACACA+AGG 0.697723 4.1:-22756022 MS.gene26573:CDS
CTGCTGAGATGGGAAAGAAG+GGG 0.698156 4.1:-22757199 None:intergenic
CAAAAGAGGAAGTAGAAGCG+AGG 0.699187 4.1:-22755184 MS.gene26573:CDS
TAGTTTGGAACGGAGAACGG+TGG 0.711322 4.1:+22754394 None:intergenic
GCTTTCGGAGAGGTAACTCG+CGG 0.726232 4.1:+22756054 None:intergenic
GAATGTTCATCAACACAAGG+AGG 0.727547 4.1:-22756019 MS.gene26573:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTCTTAATATTATCAA+AGG + chr4.1:22754489-22754508 None:intergenic 10.0%
!!! TTTTATTTATGCTTTTATTT+TGG - chr4.1:22756195-22756214 MS.gene26573:CDS 10.0%
!! AAAAATCTTAGATTTAGAAA+TGG + chr4.1:22756275-22756294 None:intergenic 15.0%
!! AAAATCTTAGATTTAGAAAT+GGG + chr4.1:22756274-22756293 None:intergenic 15.0%
!! CTAATTTATTTGTGATAATT+TGG - chr4.1:22756003-22756022 MS.gene26573:CDS 15.0%
!! TAAGATAAGTATTAAGTATT+TGG - chr4.1:22755514-22755533 MS.gene26573:CDS 15.0%
!!! AATTAGTCTGATTTATATAA+AGG + chr4.1:22755989-22756008 None:intergenic 15.0%
!!! ATTATTTGAAATATTGCAAA+AGG - chr4.1:22754646-22754665 MS.gene26573:CDS 15.0%
!!! ATTTTTGTTTCGATTAAAAT+AGG + chr4.1:22756242-22756261 None:intergenic 15.0%
!!! GTTACTTTATATAAAATTGA+TGG + chr4.1:22755910-22755929 None:intergenic 15.0%
!! AAAAAAGCTGGTAAAATTAA+TGG + chr4.1:22755568-22755587 None:intergenic 20.0%
!! AAAGCATAAATAAAAAGTCT+AGG + chr4.1:22756190-22756209 None:intergenic 20.0%
!! ATAACTTAAAACAACTTCAA+AGG + chr4.1:22754842-22754861 None:intergenic 20.0%
!! TCATAAAAAATTAGCTAAAC+TGG + chr4.1:22756338-22756357 None:intergenic 20.0%
!!! AAGTATTTGGTTTCTAAATT+TGG - chr4.1:22755527-22755546 MS.gene26573:CDS 20.0%
!!! TCTTATAATTGGTTTTCTTA+TGG - chr4.1:22754228-22754247 MS.gene26573:CDS 20.0%
! ATATGTGTCATATCCTTAAA+TGG + chr4.1:22754276-22754295 None:intergenic 25.0%
! ATGATGTAAGATAAGAATGA+TGG + chr4.1:22754698-22754717 None:intergenic 25.0%
! ATTAAGCATTTCACATTTCT+CGG + chr4.1:22754179-22754198 None:intergenic 25.0%
! CCAAAATTGTTAACCATTTA+AGG - chr4.1:22754260-22754279 MS.gene26573:CDS 25.0%
! CTTAATACTTATCTTAGATG+AGG + chr4.1:22755510-22755529 None:intergenic 25.0%
! GAAATATTGCAAAAGGATTT+AGG - chr4.1:22754653-22754672 MS.gene26573:CDS 25.0%
! TAGCATATTCATTAATGATG+TGG - chr4.1:22756360-22756379 MS.gene26573:intron 25.0%
! TGACAAAAAAATTAAGATGC+AGG + chr4.1:22754592-22754611 None:intergenic 25.0%
! TGATGTAAGATAAGAATGAT+GGG + chr4.1:22754697-22754716 None:intergenic 25.0%
! TTAATAAGCATGATTAGACT+AGG + chr4.1:22754908-22754927 None:intergenic 25.0%
! TTTGTTTCGATTAAAATAGG+TGG + chr4.1:22756239-22756258 None:intergenic 25.0%
!! CCTTAAATGGTTAACAATTT+TGG + chr4.1:22754263-22754282 None:intergenic 25.0%
!!! AATAGAATTTAGCCTTTTTG+AGG - chr4.1:22755382-22755401 MS.gene26573:intron 25.0%
!!! AATCAACCGAAATTTTGATA+CGG + chr4.1:22754546-22754565 None:intergenic 25.0%
!!! TCATTATTTTACTACGAATC+TGG - chr4.1:22754800-22754819 MS.gene26573:CDS 25.0%
!!! TTTTTTGTCAATAACAGTTC+AGG + chr4.1:22754426-22754445 None:intergenic 25.0%
AAATTAGCTAAACTGGATTG+TGG + chr4.1:22756331-22756350 None:intergenic 30.0%
AACAACAAGTACTTGCAATT+AGG - chr4.1:22755069-22755088 MS.gene26573:intron 30.0%
AATTCCCGAATATGTTTGTA+AGG + chr4.1:22755414-22755433 None:intergenic 30.0%
ACTATGTTAACAGTTATGGA+AGG - chr4.1:22756442-22756461 MS.gene26573:intron 30.0%
AGTAATCCAAGAGAAAGATT+TGG - chr4.1:22756938-22756957 MS.gene26573:intron 30.0%
AGTATCCTAACTGTAATATG+TGG - chr4.1:22755605-22755624 MS.gene26573:CDS 30.0%
ATCATGCTTATTAACATAGC+AGG - chr4.1:22754914-22754933 MS.gene26573:intron 30.0%
CTTGTACCGTATCAAAATTT+CGG - chr4.1:22754537-22754556 MS.gene26573:CDS 30.0%
GGACCTTGATATGAATTAAA+AGG + chr4.1:22754571-22754590 None:intergenic 30.0%
GGTCATTCAAATGGAATAAA+GGG - chr4.1:22757041-22757060 MS.gene26573:intron 30.0%
GTATCAAAATTTCGGTTGAT+TGG - chr4.1:22754545-22754564 MS.gene26573:CDS 30.0%
GTTCTCCTTACAAACATATT+CGG - chr4.1:22755406-22755425 MS.gene26573:intron 30.0%
GTTGACTATGTTAACAGTTA+TGG - chr4.1:22756438-22756457 MS.gene26573:intron 30.0%
TCTAGAAATAAGGATTCTCA+TGG - chr4.1:22757073-22757092 MS.gene26573:intron 30.0%
TGGAATGTTCATAACTCATA+AGG + chr4.1:22754515-22754534 None:intergenic 30.0%
TGTGAATTGGAAATTCTTCA+GGG + chr4.1:22756582-22756601 None:intergenic 30.0%
TTCATGATCGATAAAAAAGC+TGG + chr4.1:22755580-22755599 None:intergenic 30.0%
TTCTCCTTACAAACATATTC+GGG - chr4.1:22755407-22755426 MS.gene26573:intron 30.0%
! AATATTTTCTCAATGCTGCT+TGG + chr4.1:22755032-22755051 None:intergenic 30.0%
! AGCAGCATTGAGAAAATATT+TGG - chr4.1:22755032-22755051 MS.gene26573:intron 30.0%
! CAGCATTGAGAAAATATTTG+GGG - chr4.1:22755034-22755053 MS.gene26573:intron 30.0%
! GCAGCATTGAGAAAATATTT+GGG - chr4.1:22755033-22755052 MS.gene26573:intron 30.0%
! GGTAAAATTAATGGATCTAG+CGG + chr4.1:22755559-22755578 None:intergenic 30.0%
! GTCAATGTTCTCTAGAAATA+AGG - chr4.1:22757063-22757082 MS.gene26573:intron 30.0%
! GTTTTTACACATCACTCTAA+GGG - chr4.1:22754114-22754133 MS.gene26573:CDS 30.0%
! TGGAATTTTTGTAAAGTCTG+AGG - chr4.1:22756023-22756042 MS.gene26573:CDS 30.0%
!! TGGCCTTTTAATTCATATCA+AGG - chr4.1:22754565-22754584 MS.gene26573:CDS 30.0%
!!! ATAGTCGATCTTGTTGATTT+TGG + chr4.1:22756698-22756717 None:intergenic 30.0%
AAGGAATAGGGTCATTCAAA+TGG - chr4.1:22757032-22757051 MS.gene26573:intron 35.0%
AATCCAAGAGAAAGATTTGG+CGG - chr4.1:22756941-22756960 MS.gene26573:intron 35.0%
AATCCAGATGTTAGAAAACC+AGG - chr4.1:22755787-22755806 MS.gene26573:intron 35.0%
ACAGTCCATTCTCATCAAAT+TGG + chr4.1:22754456-22754475 None:intergenic 35.0%
ACGTACCACATATTACAGTT+AGG + chr4.1:22755613-22755632 None:intergenic 35.0%
AGATTCAAACCATGTTCTTG+AGG + chr4.1:22754300-22754319 None:intergenic 35.0%
AGCACAAAAGAGAAGAAGAA+AGG - chr4.1:22754951-22754970 MS.gene26573:intron 35.0%
ATAAAAAGTCTAGGCTGTGT+TGG + chr4.1:22756181-22756200 None:intergenic 35.0%
ATCTATTGGAAATGTGAGTG+TGG - chr4.1:22757132-22757151 MS.gene26573:intron 35.0%
ATGTGGTACGTGCAATATAT+AGG - chr4.1:22755622-22755641 MS.gene26573:CDS 35.0%
CATCAAAAGAGAGAGATCAA+TGG - chr4.1:22756128-22756147 MS.gene26573:CDS 35.0%
CTCGTTGTGGTTCTTATAAT+TGG - chr4.1:22754217-22754236 MS.gene26573:CDS 35.0%
GATTCAAACCATGTTCTTGA+GGG + chr4.1:22754299-22754318 None:intergenic 35.0%
GGGTCATTCAAATGGAATAA+AGG - chr4.1:22757040-22757059 MS.gene26573:intron 35.0%
GTCAAAATATTCAAGGCCAA+GGG - chr4.1:22756787-22756806 MS.gene26573:intron 35.0%
GTGTGAATTGGAAATTCTTC+AGG + chr4.1:22756583-22756602 None:intergenic 35.0%
TACCAGTCACTTGAATCTAT+TGG - chr4.1:22757118-22757137 MS.gene26573:intron 35.0%
TCAAAATATTCAAGGCCAAG+GGG - chr4.1:22756788-22756807 MS.gene26573:intron 35.0%
TCAAGTGACTGGTATTTATC+TGG + chr4.1:22757112-22757131 None:intergenic 35.0%
TCCATGTTATCAAAGGCAAA+AGG + chr4.1:22756665-22756684 None:intergenic 35.0%
TCGGAATAGACAATGTAAGA+AGG + chr4.1:22754160-22754179 None:intergenic 35.0%
TGATAGCTTACTGACAAAAG+AGG - chr4.1:22756068-22756087 MS.gene26573:CDS 35.0%
TGGTGTATCCATGTTATCAA+AGG + chr4.1:22756672-22756691 None:intergenic 35.0%
TGTAGCCAATTTGATGAGAA+TGG - chr4.1:22754448-22754467 MS.gene26573:CDS 35.0%
TTCCAATAGATTCAAGTGAC+TGG + chr4.1:22757123-22757142 None:intergenic 35.0%
TTCCTTTGTCACAAGGAATA+GGG - chr4.1:22757020-22757039 MS.gene26573:intron 35.0%
TTTCCTTTGTCACAAGGAAT+AGG - chr4.1:22757019-22757038 MS.gene26573:intron 35.0%
TTTGTATGTTGTTCTCGTTG+TGG - chr4.1:22754204-22754223 MS.gene26573:CDS 35.0%
! AAAGAGAAGAAGAAAGGTGT+AGG - chr4.1:22754957-22754976 MS.gene26573:intron 35.0%
! CTTGTCTTTTGTTCCGAATA+TGG + chr4.1:22756614-22756633 None:intergenic 35.0%
! GGTTTTTACACATCACTCTA+AGG - chr4.1:22754113-22754132 MS.gene26573:CDS 35.0%
! TCTCCTTGTTTCACAAGTTT+CGG - chr4.1:22756296-22756315 MS.gene26573:CDS 35.0%
! TTTTGTTCCGAATATGGTCT+TGG + chr4.1:22756608-22756627 None:intergenic 35.0%
!! ATTTTGATACGGTACAAGCT+TGG + chr4.1:22754535-22754554 None:intergenic 35.0%
!! ATTTTTGTAAAGTCTGAGGC+AGG - chr4.1:22756027-22756046 MS.gene26573:CDS 35.0%
!! TTGATGAGAATGGACTGTAA+TGG - chr4.1:22754458-22754477 MS.gene26573:CDS 35.0%
!! TTGGTTTTCAGCAATCAAGA+GGG - chr4.1:22754092-22754111 MS.gene26573:CDS 35.0%
!! TTTCCTTGTGGAGTTCTAAA+TGG + chr4.1:22756752-22756771 None:intergenic 35.0%
!!! AAGAGTTTTAGAGCTCTGAA+AGG - chr4.1:22755650-22755669 MS.gene26573:intron 35.0%
!!! GATCTTGTTGATTTTGGAGT+TGG + chr4.1:22756692-22756711 None:intergenic 35.0%
!!! GTTCTAGATGGTTTTGAAGA+TGG + chr4.1:22756725-22756744 None:intergenic 35.0%
!!! TTTTAGAGCTCTGAAAGGTT+TGG - chr4.1:22755655-22755674 MS.gene26573:intron 35.0%
AAACGAAGTTCAGGCAAAAG+AGG + chr4.1:22754754-22754773 None:intergenic 40.0%
AAAGTCTCAGCTCAGAAAAC+AGG + chr4.1:22754369-22754388 None:intergenic 40.0%
ACACATATCCCTCAAGAACA+TGG - chr4.1:22754288-22754307 MS.gene26573:CDS 40.0%
AGACAATGTAAGAAGGCAGT+AGG + chr4.1:22754153-22754172 None:intergenic 40.0%
AGGCAGGTAACAATGAAGAT+TGG - chr4.1:22756043-22756062 MS.gene26573:CDS 40.0%
ATCAAACCCACGAGAAAAAC+AGG - chr4.1:22756556-22756575 MS.gene26573:intron 40.0%
ATTACTACTAGCGCGTACTT+TGG + chr4.1:22756924-22756943 None:intergenic 40.0%
CGTCAAAATATTCAAGGCCA+AGG - chr4.1:22756786-22756805 MS.gene26573:intron 40.0%
GAATGTTCATCAACACAAGG+AGG - chr4.1:22755247-22755266 MS.gene26573:intron 40.0%
GCGTGACGTCAAAATATTCA+AGG - chr4.1:22756780-22756799 MS.gene26573:intron 40.0%
GGCAGGTAACAATGAAGATT+GGG - chr4.1:22756044-22756063 MS.gene26573:CDS 40.0%
GGCATTTGCATATTCACATC+AGG - chr4.1:22756149-22756168 MS.gene26573:CDS 40.0%
GGTGTAGGAAAACTAAAGCA+TGG - chr4.1:22754972-22754991 MS.gene26573:intron 40.0%
GTAAGGAGAACACCTCAAAA+AGG + chr4.1:22755397-22755416 None:intergenic 40.0%
TAACAGCTGCATGTGTTTCA+TGG + chr4.1:22755735-22755754 None:intergenic 40.0%
TAAGAGGACAGAATGTGAAG+AGG - chr4.1:22755696-22755715 MS.gene26573:intron 40.0%
TCCAGCTTCATTCTTGTACT+CGG + chr4.1:22755825-22755844 None:intergenic 40.0%
TTCCCGAAACTTGTGAAACA+AGG + chr4.1:22756302-22756321 None:intergenic 40.0%
TTTACAGAACCAGCATGTCT+CGG - chr4.1:22755292-22755311 MS.gene26573:intron 40.0%
! AAAGGTTTGGTAAGGCTTCA+AGG - chr4.1:22755668-22755687 MS.gene26573:intron 40.0%
! ATGTAGTTTGGAACGGAGAA+CGG + chr4.1:22756878-22756897 None:intergenic 40.0%
! CTCCTTGTTTCACAAGTTTC+GGG - chr4.1:22756297-22756316 MS.gene26573:CDS 40.0%
! TACACATCACTCTAAGGGAA+AGG - chr4.1:22754119-22754138 MS.gene26573:CDS 40.0%
! TGGTTCTGTAAAGTTGGTTC+TGG + chr4.1:22755284-22755303 None:intergenic 40.0%
! TTTTGCCTGAACTTCGTTTC+TGG - chr4.1:22754755-22754774 MS.gene26573:CDS 40.0%
!! AATGAAGCTGGAAGTACCTT+GGG - chr4.1:22755833-22755852 MS.gene26573:intron 40.0%
!! ACATGCTGGTTCTGTAAAGT+TGG + chr4.1:22755290-22755309 None:intergenic 40.0%
!! CTAAATGGAGGAGTTCTAGA+TGG + chr4.1:22756737-22756756 None:intergenic 40.0%
!! GCCTTTTGCCTTTGATAACA+TGG - chr4.1:22756661-22756680 MS.gene26573:intron 40.0%
!!! ACAGGCGTTTGAGTTGATTT+TGG + chr4.1:22756473-22756492 None:intergenic 40.0%
!!! CGTTTGAGTTGATTTTGGAG+TGG + chr4.1:22756468-22756487 None:intergenic 40.0%
!!! GTTGGTTTTCAGCAATCAAG+AGG - chr4.1:22754091-22754110 MS.gene26573:CDS 40.0%
AAAAGTCTAGGCTGTGTTGG+TGG + chr4.1:22756178-22756197 None:intergenic 45.0%
ACAGTTGGAGCCATGTAGTT+TGG + chr4.1:22756890-22756909 None:intergenic 45.0%
ACCTTTCGACGTACCATTGA+AGG - chr4.1:22756826-22756845 MS.gene26573:intron 45.0%
AGACTGTCATCGTCCTTCAA+TGG + chr4.1:22756842-22756861 None:intergenic 45.0%
CAAAAGACAAGTCCAAGACC+TGG - chr4.1:22756623-22756642 MS.gene26573:intron 45.0%
CAAAAGAGGAAGTAGAAGCG+AGG - chr4.1:22756082-22756101 MS.gene26573:CDS 45.0%
CACACCACCAAGACCATATT+CGG - chr4.1:22756598-22756617 MS.gene26573:intron 45.0%
CTCTTGGAATCAGTGCTTGT+TGG + chr4.1:22756989-22757008 None:intergenic 45.0%
GAAGAGGGGGATGAAAGAAT+TGG + chr4.1:22755003-22755022 None:intergenic 45.0%
GACCCTATTCCTTGTGACAA+AGG + chr4.1:22757025-22757044 None:intergenic 45.0%
GCGGAATGTTCATCAACACA+AGG - chr4.1:22755244-22755263 MS.gene26573:intron 45.0%
GGCTGCTTACAGAGGTTATA+TGG - chr4.1:22755328-22755347 MS.gene26573:intron 45.0%
GTACGTGCAATATATAGGCC+AGG - chr4.1:22755627-22755646 MS.gene26573:intron 45.0%
GTCTCGTTTCCTTTGTCACA+AGG - chr4.1:22757013-22757032 MS.gene26573:intron 45.0%
GTTCTCCGTTCCAAACTACA+TGG - chr4.1:22756877-22756896 MS.gene26573:intron 45.0%
TATAACCTCTGTAAGCAGCC+TGG + chr4.1:22755328-22755347 None:intergenic 45.0%
TCCAATCACATTGAGCGTCT+CGG - chr4.1:22754390-22754409 MS.gene26573:CDS 45.0%
TCCTTCAATGGTACGTCGAA+AGG + chr4.1:22756830-22756849 None:intergenic 45.0%
TGCTTCCAGAAACGAAGTTC+AGG + chr4.1:22754763-22754782 None:intergenic 45.0%
TGGAGCCATGTAGTTTGGAA+CGG + chr4.1:22756885-22756904 None:intergenic 45.0%
TGGTACGTCGAAAGGTGAAT+TGG + chr4.1:22756822-22756841 None:intergenic 45.0%
TTTGGTAAGGCTTCAAGGAG+TGG - chr4.1:22755673-22755692 MS.gene26573:intron 45.0%
! AAAGTCTAGGCTGTGTTGGT+GGG + chr4.1:22756177-22756196 None:intergenic 45.0%
! ACGCTTGAAGTGTTTCCTTG+TGG + chr4.1:22756764-22756783 None:intergenic 45.0%
! CCTCCATTTAGAACTCCACA+AGG - chr4.1:22756746-22756765 MS.gene26573:intron 45.0%
! TAGGAGAAGCAACTCTAGGA+GGG + chr4.1:22755136-22755155 None:intergenic 45.0%
! TGTTCCGAATATGGTCTTGG+TGG + chr4.1:22756605-22756624 None:intergenic 45.0%
! TTTTCTCGTGGGTTTGATGC+AGG + chr4.1:22756554-22756573 None:intergenic 45.0%
!! ACCTTGGGGAAAAGTGCTTT+AGG - chr4.1:22755848-22755867 MS.gene26573:intron 45.0%
!! ATGAAGCTGGAAGTACCTTG+GGG - chr4.1:22755834-22755853 MS.gene26573:intron 45.0%
!! ATGGTCTTGGTGGTGTGAAT+TGG + chr4.1:22756595-22756614 None:intergenic 45.0%
!! CAACAAGCACTGATTCCAAG+AGG - chr4.1:22756987-22757006 MS.gene26573:intron 45.0%
!! CAGAGCTCTAAAACTCTTCC+TGG + chr4.1:22755648-22755667 None:intergenic 45.0%
!! CCTTGTGGAGTTCTAAATGG+AGG + chr4.1:22756749-22756768 None:intergenic 45.0%
!! GAATGAAGCTGGAAGTACCT+TGG - chr4.1:22755832-22755851 MS.gene26573:intron 45.0%
!! GAGCTCTGAAAGGTTTGGTA+AGG - chr4.1:22755660-22755679 MS.gene26573:intron 45.0%
!! GGGAAAAGTGCTTTAGGACA+TGG - chr4.1:22755854-22755873 MS.gene26573:intron 45.0%
!! GTGCCTGGTTTTCTAACATC+TGG + chr4.1:22755793-22755812 None:intergenic 45.0%
!! TCCTAAAGCACTTTTCCCCA+AGG + chr4.1:22755852-22755871 None:intergenic 45.0%
!!! TATAAAAAATAAAAGTATTT+AGG - chr4.1:22755450-22755469 MS.gene26573:CDS 5.0%
AAGATCCAGGCTGCTTACAG+AGG - chr4.1:22755320-22755339 MS.gene26573:intron 50.0%
AAGTAGAAGCGAGGTTGCAG+AGG - chr4.1:22756091-22756110 MS.gene26573:CDS 50.0%
AGATTTGGCGGTGGTAGTAG+TGG - chr4.1:22756953-22756972 MS.gene26573:intron 50.0%
AGCAGTATCCTTGCCTGCAA+GGG - chr4.1:22757159-22757178 MS.gene26573:CDS 50.0%
AGGAAACGAGACTCTCCTCT+TGG + chr4.1:22757005-22757024 None:intergenic 50.0%
AGGATTCTCATGGCTCTCAC+AGG - chr4.1:22757083-22757102 MS.gene26573:intron 50.0%
AGGCTTCAAGGAGTGGTAAG+AGG - chr4.1:22755680-22755699 MS.gene26573:intron 50.0%
CCAAGAGAAAGATTTGGCGG+TGG - chr4.1:22756944-22756963 MS.gene26573:intron 50.0%
CCACCGCCAAATCTTTCTCT+TGG + chr4.1:22756947-22756966 None:intergenic 50.0%
CTAGGAGAAGCAACTCTAGG+AGG + chr4.1:22755137-22755156 None:intergenic 50.0%
CTCCTAGAGTTGCTTCTCCT+AGG - chr4.1:22755135-22755154 MS.gene26573:CDS 50.0%
GAATTGGATCCTACTCCCCT+TGG + chr4.1:22756806-22756825 None:intergenic 50.0%
GCCGAGTACAAGAATGAAGC+TGG - chr4.1:22755821-22755840 MS.gene26573:intron 50.0%
GGCTTTCTTTCAACCCTTGC+AGG + chr4.1:22757175-22757194 None:intergenic 50.0%
TATTCAAGGCCAAGGGGAGT+AGG - chr4.1:22756794-22756813 MS.gene26573:intron 50.0%
TCCAAGGGATCCATCTCCAA+GGG - chr4.1:22755181-22755200 MS.gene26573:CDS 50.0%
TCCGAGACGCTCAATGTGAT+TGG + chr4.1:22754394-22754413 None:intergenic 50.0%
TCCTAAGCCTCCTTCTCCAA+GGG - chr4.1:22755166-22755185 MS.gene26573:CDS 50.0%
TCTTTCAACCCTTGCAGGCA+AGG + chr4.1:22757170-22757189 None:intergenic 50.0%
! CTGCTTGGTTCTCTGAAGAG+GGG + chr4.1:22755017-22755036 None:intergenic 50.0%
! GCTGCTTGGTTCTCTGAAGA+GGG + chr4.1:22755018-22755037 None:intergenic 50.0%
! TAGTTTGGAACGGAGAACGG+TGG + chr4.1:22756875-22756894 None:intergenic 50.0%
! TGCTGCTTGGTTCTCTGAAG+AGG + chr4.1:22755019-22755038 None:intergenic 50.0%
! TGCTTGGTTCTCTGAAGAGG+GGG + chr4.1:22755016-22755035 None:intergenic 50.0%
!! TCAGGGCCTGTTTTTCTCGT+GGG + chr4.1:22756565-22756584 None:intergenic 50.0%
!! TTCAGGGCCTGTTTTTCTCG+TGG + chr4.1:22756566-22756585 None:intergenic 50.0%
!! TTCCGCGAAGATGCTGCTTT+CGG + chr4.1:22755230-22755249 None:intergenic 50.0%
!! TTGGCCTTGGCAGAAACAGT+TGG + chr4.1:22756905-22756924 None:intergenic 50.0%
ACTAGCGCGTACTTTGGCCT+TGG + chr4.1:22756918-22756937 None:intergenic 55.0%
AGAAGCGAGGTTGCAGAGGA+AGG - chr4.1:22756095-22756114 MS.gene26573:CDS 55.0%
AGAGGTAACTCGCGGAGAAG+CGG + chr4.1:22755207-22755226 None:intergenic 55.0%
CAGCAGTATCCTTGCCTGCA+AGG - chr4.1:22757158-22757177 MS.gene26573:CDS 55.0%
CTCCAAGGGATCCATCTCCA+AGG - chr4.1:22755180-22755199 MS.gene26573:CDS 55.0%
CTCCGAAAGCAGCATCTTCG+CGG - chr4.1:22755225-22755244 MS.gene26573:CDS 55.0%
CTCCTAAGCCTCCTTCTCCA+AGG - chr4.1:22755165-22755184 MS.gene26573:CDS 55.0%
CTCGATCGCGTATCTGTCAC+AGG + chr4.1:22756491-22756510 None:intergenic 55.0%
GAAGGAGGCTTAGGAGAAGG+AGG + chr4.1:22755161-22755180 None:intergenic 55.0%
GACCTGGCTCAAGCTCTCAA+AGG - chr4.1:22756639-22756658 MS.gene26573:intron 55.0%
GATGGATCCCTTGGAGAAGG+AGG + chr4.1:22755176-22755195 None:intergenic 55.0%
GCTTTCGGAGAGGTAACTCG+CGG + chr4.1:22755215-22755234 None:intergenic 55.0%
GGAGAAGGAGGCTTAGGAGA+AGG + chr4.1:22755164-22755183 None:intergenic 55.0%
GGAGATGGATCCCTTGGAGA+AGG + chr4.1:22755179-22755198 None:intergenic 55.0%
GGCCTAGGAGAAGCAACTCT+AGG + chr4.1:22755140-22755159 None:intergenic 55.0%
GTGACAGATACGCGATCGAG+CGG - chr4.1:22756491-22756510 MS.gene26573:intron 55.0%
TCCCTTGGAGAAGGAGGCTT+AGG + chr4.1:22755170-22755189 None:intergenic 55.0%
TCTTAGTCGCCGAGACATGC+TGG + chr4.1:22755304-22755323 None:intergenic 55.0%
TGAGAGCTTGAGCCAGGTCT+TGG + chr4.1:22756638-22756657 None:intergenic 55.0%
TGTCTCGGCGACTAAGATCC+AGG - chr4.1:22755307-22755326 MS.gene26573:intron 55.0%
! ACTCGGCTTGATATCGTGCC+TGG + chr4.1:22755808-22755827 None:intergenic 55.0%
! ACTCTAGGAGGGACGAAAGG+TGG + chr4.1:22755125-22755144 None:intergenic 55.0%
! GCAACTCTAGGAGGGACGAA+AGG + chr4.1:22755128-22755147 None:intergenic 55.0%
! GGGACGAAAGGTGGCGTTTT+TGG + chr4.1:22755116-22755135 None:intergenic 55.0%
!! CGAAGATGCTGCTTTCGGAG+AGG + chr4.1:22755225-22755244 None:intergenic 55.0%
!! GGCTCCAACTGTTTCTGCCA+AGG - chr4.1:22756898-22756917 MS.gene26573:intron 55.0%
!!! AGGTGGCGTTTTTGGTCGCT+CGG + chr4.1:22755108-22755127 None:intergenic 55.0%
CCAACCAGCTCCACCACCAT+GGG + chr4.1:22756520-22756539 None:intergenic 60.0%
GCCCTTGGAGATGGATCCCT+TGG + chr4.1:22755185-22755204 None:intergenic 60.0%
GGCTTAGGAGAAGGAGGCCT+AGG + chr4.1:22755155-22755174 None:intergenic 60.0%
TCCAACCAGCTCCACCACCA+TGG + chr4.1:22756521-22756540 None:intergenic 60.0%
! GATCGAGCGGATTCCCATGG+TGG - chr4.1:22756504-22756523 MS.gene26573:intron 60.0%
! GGCCTTTGAGAGCTTGAGCC+AGG + chr4.1:22756644-22756663 None:intergenic 60.0%
! TTTGGTCGCTCGGGAGGTGT+GGG + chr4.1:22755098-22755117 None:intergenic 60.0%
! TTTTGGTCGCTCGGGAGGTG+TGG + chr4.1:22755099-22755118 None:intergenic 60.0%
!! GATTCCCATGGTGGTGGAGC+TGG - chr4.1:22756513-22756532 MS.gene26573:intron 60.0%
!!! GGTGGCGTTTTTGGTCGCTC+GGG + chr4.1:22755107-22755126 None:intergenic 60.0%
GGAGAAGCGGCCCTTGGAGA+TGG + chr4.1:22755194-22755213 None:intergenic 65.0%
! CGAGCGGATTCCCATGGTGG+TGG - chr4.1:22756507-22756526 MS.gene26573:intron 65.0%
! CGCGATCGAGCGGATTCCCA+TGG - chr4.1:22756501-22756520 MS.gene26573:intron 65.0%
!! CCCATGGTGGTGGAGCTGGT+TGG - chr4.1:22756517-22756536 MS.gene26573:intron 65.0%
!!! GGCGTTTTTGGTCGCTCGGG+AGG + chr4.1:22755104-22755123 None:intergenic 65.0%
ACTCGCGGAGAAGCGGCCCT+TGG + chr4.1:22755200-22755219 None:intergenic 70.0%
! GGTCGCTCGGGAGGTGTGGG+AGG + chr4.1:22755095-22755114 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 22754075 22757213 22754075 ID=MS.gene26573
chr4.1 mRNA 22754075 22757213 22754075 ID=MS.gene26573.t1;Parent=MS.gene26573
chr4.1 exon 22757148 22757213 22757148 ID=MS.gene26573.t1.exon1;Parent=MS.gene26573.t1
chr4.1 CDS 22757148 22757213 22757148 ID=cds.MS.gene26573.t1;Parent=MS.gene26573.t1
chr4.1 exon 22755939 22756352 22755939 ID=MS.gene26573.t1.exon2;Parent=MS.gene26573.t1
chr4.1 CDS 22755939 22756352 22755939 ID=cds.MS.gene26573.t1;Parent=MS.gene26573.t1
chr4.1 exon 22755411 22755644 22755411 ID=MS.gene26573.t1.exon3;Parent=MS.gene26573.t1
chr4.1 CDS 22755411 22755644 22755411 ID=cds.MS.gene26573.t1;Parent=MS.gene26573.t1
chr4.1 exon 22755118 22755249 22755118 ID=MS.gene26573.t1.exon4;Parent=MS.gene26573.t1
chr4.1 CDS 22755118 22755249 22755118 ID=cds.MS.gene26573.t1;Parent=MS.gene26573.t1
chr4.1 exon 22754075 22754833 22754075 ID=MS.gene26573.t1.exon5;Parent=MS.gene26573.t1
chr4.1 CDS 22754075 22754833 22754075 ID=cds.MS.gene26573.t1;Parent=MS.gene26573.t1
Gene Sequence

>MS.gene26573

ATGGGAAAGAAGGGGAGTTGGTTTTCAGCAATCAAGAGGGTTTTTACACATCACTCTAAGGGAAAGGTAATTTATCCTACTGCCTTCTTACATTGTCTATTCCGAGAAATGTGAAATGCTTAATTTGTTTTTGTATGTTGTTCTCGTTGTGGTTCTTATAATTGGTTTTCTTATGGCATTAGATGCCAAAATTGTTAACCATTTAAGGATATGACACATATCCCTCAAGAACATGGTTTGAATCTCATCACTACTGATGTGAAGATTTCATGATAGAGTAGTTTGTAAGTACCTGTTTTCTGAGCTGAGACTTTTTCCAATCACATTGAGCGTCTCGGATTCAACTCACCTGAACTGTTATTGACAAAAAAGTTGTAGCCAATTTGATGAGAATGGACTGTAATGGATAGACCTTTGATAATATTAAGAAAATTCTTCCTTATGAGTTATGAACATTCCAAGCTTGTACCGTATCAAAATTTCGGTTGATTGGCCTTTTAATTCATATCAAGGTCCTGCATCTTAATTTTTTTGTCAAATGTCAATTAGTTTTGTTCGTAACTACTAATAGATTATTTGAAATATTGCAAAAGGATTTAGGATATTGCTTTTAATTCTTCCCATCATTCTTATCTTACATCATATTTGCTTTATGCATTAGTTTTAAATTATTTTACCTCTTTTGCCTGAACTTCGTTTCTGGAAGCAACATATGAATGATGATATCATTATTTTACTACGAATCTGGTGTTTTCGTTATTCTGCCTTTGAAGTTGTTTTAAGTTATGTTACTGAATTTGAACTTGTTTCAGTTCTTCATATTGATCTATCCTAGTCTAATCATGCTTATTAACATAGCAGGATTCAGAAAACAAAAGCACAAAAGAGAAGAAGAAAGGTGTAGGAAAACTAAAGCATGGAGAGACCAATTCTTTCATCCCCCTCTTCAGAGAACCAAGCAGCATTGAGAAAATATTTGGGGACTTTGAAAGAGAACAACAAGTACTTGCAATTAGGCCTCCCACACCTCCCGAGCGACCAAAAACGCCACCTTTCGTCCCTCCTAGAGTTGCTTCTCCTAGGCCTCCTTCTCCTAAGCCTCCTTCTCCAAGGGATCCATCTCCAAGGGCCGCTTCTCCGCGAGTTACCTCTCCGAAAGCAGCATCTTCGCGGAATGTTCATCAACACAAGGAGGTTCGCAACAGACCAGAACCAACTTTACAGAACCAGCATGTCTCGGCGACTAAGATCCAGGCTGCTTACAGAGGTTATATGGTAAGCTTTTATATTTTATTGTTAATGTAATTAATAGAATTTAGCCTTTTTGAGGTGTTCTCCTTACAAACATATTCGGGAATTAAAATAGAAACTATAATATAAAAAATAAAAGTATTTAGGTTAAGTTTTGATTAACTTTTAGTATATGAGAGTACCTCATCTAAGATAAGTATTAAGTATTTGGTTTCTAAATTTGGAAAATGCCGCTAGATCCATTAATTTTACCAGCTTTTTTATCGATCATGAAACAAAAGTATCCTAACTGTAATATGTGGTACGTGCAATATATAGGCCAGGAAGAGTTTTAGAGCTCTGAAAGGTTTGGTAAGGCTTCAAGGAGTGGTAAGAGGACAGAATGTGAAGAGGCAGACAGTTAATGCCATGAAACACATGCAGCTGTTAGTGCGAGTTCAGTCTCAGATTCAATCTCGAAGAATCCAGATGTTAGAAAACCAGGCACGATATCAAGCCGAGTACAAGAATGAAGCTGGAAGTACCTTGGGGAAAAGTGCTTTAGGACATGGTGTAAGTTATCATACTCTTCTTTATCGTATCCATCAATTTTATATAAAGTAACTTCTTAATATCTAATACTTCTATTCTGAAATATGTGCATGATTATATTTTCAACTGCCTTTATATAAATCAGACTAATTTATTTGTGATAATTTGGAATTTTTGTAAAGTCTGAGGCAGGTAACAATGAAGATTGGGATGATAGCTTACTGACAAAAGAGGAAGTAGAAGCGAGGTTGCAGAGGAAGGTTGAAGCAATCATCAAAAGAGAGAGATCAATGGCATTTGCATATTCACATCAGGTACCCACCAACACAGCCTAGACTTTTTATTTATGCTTTTATTTTGGCTTAAATACGCTTATTATCCACCTATTTTAATCGAAACAAAAATTTCAGTACACCCATTTCTAAATCTAAGATTTTTGTCTCCTTGTTTCACAAGTTTCGGGAACTTTTTCCACAATCCAGTTTAGCTAATTTTTTATGATGTAGCATATTCATTAATGATGTGGAATTATATTTTATGATGCGACGTTGCTAATTTAGATCACTATTTGTTATGACTTTGTTGACTATGTTAACAGTTATGGAAGGCCACTCCAAAATCAACTCAAACGCCTGTGACAGATACGCGATCGAGCGGATTCCCATGGTGGTGGAGCTGGTTGGAAAGACAATTACCTGCATCAAACCCACGAGAAAAACAGGCCCTGAAGAATTTCCAATTCACACCACCAAGACCATATTCGGAACAAAAGACAAGTCCAAGACCTGGCTCAAGCTCTCAAAGGCCTTTTGCCTTTGATAACATGGATACACCAACTCCAAAATCAACAAGATCGACTATATTTCCATCTTCAAAACCATCTAGAACTCCTCCATTTAGAACTCCACAAGGAAACACTTCAAGCGTGACGTCAAAATATTCAAGGCCAAGGGGAGTAGGATCCAATTCACCTTTCGACGTACCATTGAAGGACGATGACAGTCTCACAAGCTGTCCACCGTTCTCCGTTCCAAACTACATGGCTCCAACTGTTTCTGCCAAGGCCAAAGTACGCGCTAGTAGTAATCCAAGAGAAAGATTTGGCGGTGGTAGTAGTGGCTGTGCAACTCCAACAAGCACTGATTCCAAGAGGAGAGTCTCGTTTCCTTTGTCACAAGGAATAGGGTCATTCAAATGGAATAAAGGGTCAATGTTCTCTAGAAATAAGGATTCTCATGGCTCTCACAGGACTCCAGATAAATACCAGTCACTTGAATCTATTGGAAATGTGAGTGTGGATTCAGCAGTATCCTTGCCTGCAAGGGTTGAAAGAAAGCCTTTTACAAGATTTGTGTGA
Protein sequence

>MS.gene26573.t1

MGKKGSWFSAIKRVFTHHSKGKDSENKSTKEKKKGVGKLKHGETNSFIPLFREPSSIEKIFGDFEREQQVLAIRPPTPPERPKTPPFVPPRVASPRPPSPKPPSPRDPSPRAASPRVTSPKAASSRNVHQHKEVRNRPEPTLQNQHVSATKIQAAYRGYMARKSFRALKGLVRLQGVVRGQNVKRQTVNAMKHMQLLVRVQSQIQSRRIQMLENQARYQAEYKNEAGSTLGKSALGHGSEAGNNEDWDDSLLTKEEVEARLQRKVEAIIKRERSMAFAYSHQLWKATPKSTQTPVTDTRSSGFPWWWSWLERQLPASNPREKQALKNFQFTPPRPYSEQKTSPRPGSSSQRPFAFDNMDTPTPKSTRSTIFPSSKPSRTPPFRTPQGNTSSVTSKYSRPRGVGSNSPFDVPLKDDDSLTSCPPFSVPNYMAPTVSAKAKVRASSNPRERFGGGSSGCATPTSTDSKRRVSFPLSQGIGSFKWNKGSMFSRNKDSHGSHRTPDKYQSLESIGNVSVDSAVSLPARVERKPFTRFV