AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060338


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACAGCTTCTTGCATTAA+AGG 0.122689 8.3:+10848978 None:intergenic
GTGAGCAATTGTGGGCAATA+AGG 0.360166 8.3:-10848869 MS.gene060338:CDS
CTTAATGTAACTCGCTCTTC+AGG 0.364661 8.3:+10850151 None:intergenic
TCAGGTCTATCTGATCTTAC+TGG 0.367030 8.3:+10848906 None:intergenic
TGCTGTGAAGATGTTTGATT+TGG 0.399908 8.3:-10848946 MS.gene060338:CDS
TGAGCAATTGTGGGCAATAA+GGG 0.419425 8.3:-10848868 MS.gene060338:CDS
TCCTTCATTATATTTCCTGA+AGG 0.511707 8.3:+10849022 None:intergenic
AAGAGCGAGTTACATTAAGA+TGG 0.522640 8.3:-10850147 MS.gene060338:CDS
AAACAGTGGGTGAGCAATTG+TGG 0.527503 8.3:-10848878 MS.gene060338:CDS
GAAATATAATGAAGGAAATG+TGG 0.548375 8.3:-10849015 MS.gene060338:CDS
CAGTTGTGCTACTACTCTTG+AGG 0.565502 8.3:+10848838 None:intergenic
TCTGACTGGCCGTCGCCCTG+TGG 0.576329 8.3:-10850188 MS.gene060338:CDS
AACAGTGGGTGAGCAATTGT+GGG 0.589188 8.3:-10848877 MS.gene060338:CDS
GCCTTCAGGAAATATAATGA+AGG 0.589672 8.3:-10849023 MS.gene060338:CDS
ATATAATGAAGGAAATGTGG+TGG 0.592211 8.3:-10849012 MS.gene060338:CDS
AGAGCGAGTTACATTAAGAT+GGG 0.606183 8.3:-10850146 MS.gene060338:intron
AGAGTAGTAGCACAACTGCA+AGG 0.643008 8.3:-10848833 MS.gene060338:CDS
AGACCTGACATGAAAACAGT+GGG 0.650314 8.3:-10848891 MS.gene060338:CDS
TAGACCTGACATGAAAACAG+TGG 0.699696 8.3:-10848892 MS.gene060338:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATATAATTTAATAGGC+TGG - chr8.3:10848980-10848999 MS.gene060338:CDS 15.0%
!! AGAAATTATTCTTAACTTAG+AGG + chr8.3:10849075-10849094 None:intergenic 20.0%
!! TTGTTACTATCCTAAAATTT+GGG - chr8.3:10849420-10849439 MS.gene060338:intron 20.0%
!! TTTGTTACTATCCTAAAATT+TGG - chr8.3:10849419-10849438 MS.gene060338:intron 20.0%
!!! CCTATAAAATCGATTTTTAA+GGG - chr8.3:10849461-10849480 MS.gene060338:intron 20.0%
!!! CCTTAAAAATCGATTTTATA+GGG + chr8.3:10849463-10849482 None:intergenic 20.0%
!!! TTCGGAAAAATTGATAATTT+TGG - chr8.3:10849103-10849122 MS.gene060338:intron 20.0%
! GAAATATAATGAAGGAAATG+TGG - chr8.3:10850001-10850020 MS.gene060338:intron 25.0%
! GAATAATTTGATATGAGTTG+CGG - chr8.3:10849142-10849161 MS.gene060338:intron 25.0%
! GATCTAAAAAGATCAATGAT+AGG - chr8.3:10849395-10849414 MS.gene060338:intron 25.0%
! GTAGTAATAGATGAAAAACT+TGG - chr8.3:10849913-10849932 MS.gene060338:intron 25.0%
! TTATATTCTTAACCAGAAGT+TGG + chr8.3:10849013-10849032 None:intergenic 25.0%
! TTCATATCTATAGTCAATGT+GGG - chr8.3:10849295-10849314 MS.gene060338:intron 25.0%
!! AAAATCCTTTTGGATTGAAT+CGG + chr8.3:10849169-10849188 None:intergenic 25.0%
!! CAATTGATACAAAATCCTTT+TGG + chr8.3:10849179-10849198 None:intergenic 25.0%
!! TCATTTTAAACCAATTTTGC+AGG + chr8.3:10849548-10849567 None:intergenic 25.0%
!! TGCAAAATTGGTTTAAAATG+AGG - chr8.3:10849547-10849566 MS.gene060338:intron 25.0%
!!! ATAAAATCGATTTTTAAGGG+AGG - chr8.3:10849464-10849483 MS.gene060338:intron 25.0%
!!! CCCTATAAAATCGATTTTTA+AGG - chr8.3:10849460-10849479 MS.gene060338:intron 25.0%
!!! CCCTTAAAAATCGATTTTAT+AGG + chr8.3:10849464-10849483 None:intergenic 25.0%
!!! GTAGTCTTGATTATAAGTTT+TGG + chr8.3:10849275-10849294 None:intergenic 25.0%
AGTAGTGCTTGTAACTAATA+AGG + chr8.3:10849672-10849691 None:intergenic 30.0%
ATATAATGAAGGAAATGTGG+TGG - chr8.3:10850004-10850023 MS.gene060338:intron 30.0%
ATTAGTTACAAGCACTACTT+AGG - chr8.3:10849673-10849692 MS.gene060338:intron 30.0%
ATTTCTCAAACCTGCAAAAT+TGG - chr8.3:10849535-10849554 MS.gene060338:intron 30.0%
CTTCATATCTATAGTCAATG+TGG - chr8.3:10849294-10849313 MS.gene060338:intron 30.0%
GCTTCTTCGCAAAAAAAAAA+AGG + chr8.3:10849583-10849602 None:intergenic 30.0%
TATCCTAAAATTTGGGCTTA+GGG - chr8.3:10849427-10849446 MS.gene060338:intron 30.0%
TCACAAAACTGACATGTAAA+GGG - chr8.3:10849241-10849260 MS.gene060338:intron 30.0%
TCCTTCATTATATTTCCTGA+AGG + chr8.3:10849997-10850016 None:intergenic 30.0%
TCTATCCTAGATCTCTTATA+AGG + chr8.3:10849500-10849519 None:intergenic 30.0%
TGATGAGAGAAAATTGTGAT+TGG - chr8.3:10849197-10849216 MS.gene060338:intron 30.0%
TGCTTGTAACTAATAAGGTT+TGG + chr8.3:10849667-10849686 None:intergenic 30.0%
TGGAATAGATCTTGTAAGTT+AGG - chr8.3:10849217-10849236 MS.gene060338:intron 30.0%
TTAACTATCAATTTAAGCGC+TGG - chr8.3:10848939-10848958 MS.gene060338:CDS 30.0%
TTCGCAAAAAAAAAAAGGCT+TGG + chr8.3:10849578-10849597 None:intergenic 30.0%
TTTACAGCTTCTTGCATTAA+AGG + chr8.3:10850041-10850060 None:intergenic 30.0%
! AATAATTTCTGCTGTCACTT+CGG - chr8.3:10849085-10849104 MS.gene060338:intron 30.0%
! TATGATTCTGATCTTTTTGC+AGG - chr8.3:10849971-10849990 MS.gene060338:intron 30.0%
AAGAGCGAGTTACATTAAGA+TGG - chr8.3:10848869-10848888 MS.gene060338:CDS 35.0%
AGAGCGAGTTACATTAAGAT+GGG - chr8.3:10848870-10848889 MS.gene060338:CDS 35.0%
ATTTGGAGAATGGACACATA+TGG - chr8.3:10849353-10849372 MS.gene060338:intron 35.0%
CCAAACCTTATAAGAGATCT+AGG - chr8.3:10849492-10849511 MS.gene060338:intron 35.0%
CCTAGATCTCTTATAAGGTT+TGG + chr8.3:10849495-10849514 None:intergenic 35.0%
CTATCCTAAAATTTGGGCTT+AGG - chr8.3:10849426-10849445 MS.gene060338:intron 35.0%
CTCACAAAACTGACATGTAA+AGG - chr8.3:10849240-10849259 MS.gene060338:intron 35.0%
GCCTTCAGGAAATATAATGA+AGG - chr8.3:10849993-10850012 MS.gene060338:intron 35.0%
GTCATATCTCTAGTAGAAGT+GGG - chr8.3:10849695-10849714 MS.gene060338:intron 35.0%
TCATATCTCTAGTAGAAGTG+GGG - chr8.3:10849696-10849715 MS.gene060338:intron 35.0%
TCTAGTGAACTAGTAATCGT+GGG - chr8.3:10849853-10849872 MS.gene060338:intron 35.0%
TCTTAACTTAGAGGATCACT+TGG + chr8.3:10849066-10849085 None:intergenic 35.0%
TGCAACTACAGTTCTTCAAA+GGG + chr8.3:10849748-10849767 None:intergenic 35.0%
TGCTGTGAAGATGTTTGATT+TGG - chr8.3:10850070-10850089 MS.gene060338:intron 35.0%
TTCTAGTGAACTAGTAATCG+TGG - chr8.3:10849852-10849871 MS.gene060338:intron 35.0%
TTGCAACTACAGTTCTTCAA+AGG + chr8.3:10849749-10849768 None:intergenic 35.0%
TTTGGAGAATGGACACATAT+GGG - chr8.3:10849354-10849373 MS.gene060338:intron 35.0%
! TAGTTGCAACTTGTTCTCTT+TGG - chr8.3:10849760-10849779 MS.gene060338:intron 35.0%
! TTTTGGTCGTTATTCTCAGT+GGG - chr8.3:10849120-10849139 MS.gene060338:intron 35.0%
!! ATTTTGGTCGTTATTCTCAG+TGG - chr8.3:10849119-10849138 MS.gene060338:intron 35.0%
AGACCTGACATGAAAACAGT+GGG - chr8.3:10850125-10850144 MS.gene060338:intron 40.0%
ATTCTTAACCAGAAGTTGGC+TGG + chr8.3:10849009-10849028 None:intergenic 40.0%
CTTAATGTAACTCGCTCTTC+AGG + chr8.3:10848868-10848887 None:intergenic 40.0%
GCAACTACAGTTCTTCAAAG+GGG + chr8.3:10849747-10849766 None:intergenic 40.0%
GGTCATATCTCTAGTAGAAG+TGG - chr8.3:10849694-10849713 MS.gene060338:intron 40.0%
GTGAGTGCTTAAGAATGAAG+AGG + chr8.3:10849947-10849966 None:intergenic 40.0%
GTGTCCATTCTCCAAATGTA+CGG + chr8.3:10849350-10849369 None:intergenic 40.0%
GTTCTCTTTGGACGTGTTTA+CGG - chr8.3:10849772-10849791 MS.gene060338:intron 40.0%
TAGACCTGACATGAAAACAG+TGG - chr8.3:10850124-10850143 MS.gene060338:intron 40.0%
TCAGGTCTATCTGATCTTAC+TGG + chr8.3:10850113-10850132 None:intergenic 40.0%
TGAGCAATTGTGGGCAATAA+GGG - chr8.3:10850148-10850167 MS.gene060338:CDS 40.0%
TTCTCTTTGGACGTGTTTAC+GGG - chr8.3:10849773-10849792 MS.gene060338:intron 40.0%
TTTACGGGCATTAGCAACAT+GGG - chr8.3:10849788-10849807 MS.gene060338:intron 40.0%
! AGACCCTAAGCCCAAATTTT+AGG + chr8.3:10849433-10849452 None:intergenic 40.0%
AAACAGTGGGTGAGCAATTG+TGG - chr8.3:10850138-10850157 MS.gene060338:intron 45.0%
AACAGTGGGTGAGCAATTGT+GGG - chr8.3:10850139-10850158 MS.gene060338:intron 45.0%
AGAATGGACACATATGGGTC+GGG - chr8.3:10849359-10849378 MS.gene060338:intron 45.0%
AGAGTAGTAGCACAACTGCA+AGG - chr8.3:10850183-10850202 MS.gene060338:CDS 45.0%
AGGACCGTACATTTGGAGAA+TGG - chr8.3:10849343-10849362 MS.gene060338:intron 45.0%
ATTCCTCCCGGTACCAATTT+GGG + chr8.3:10849618-10849637 None:intergenic 45.0%
CTAAAGTAGCCCACCCAAAT+TGG - chr8.3:10849602-10849621 MS.gene060338:intron 45.0%
GAGAATGGACACATATGGGT+CGG - chr8.3:10849358-10849377 MS.gene060338:intron 45.0%
GTGAACTAGTAATCGTGGGA+CGG - chr8.3:10849857-10849876 MS.gene060338:intron 45.0%
GTGAGCAATTGTGGGCAATA+AGG - chr8.3:10850147-10850166 MS.gene060338:CDS 45.0%
GTTTACGGGCATTAGCAACA+TGG - chr8.3:10849787-10849806 MS.gene060338:intron 45.0%
TTCTCCAAATGTACGGTCCT+AGG + chr8.3:10849343-10849362 None:intergenic 45.0%
! CAGTTGTGCTACTACTCTTG+AGG + chr8.3:10850181-10850200 None:intergenic 45.0%
! TCACCCACTGTTTTCATGTC+AGG + chr8.3:10850131-10850150 None:intergenic 45.0%
! TGATCTTTTTGCAGGCCTTC+AGG - chr8.3:10849979-10849998 MS.gene060338:intron 45.0%
!!! GACCTTTTTTCAGCTCCACA+GGG + chr8.3:10848846-10848865 None:intergenic 45.0%
!! ATGATATAATATAATTTAAT+AGG - chr8.3:10848976-10848995 MS.gene060338:CDS 5.0%
AGGTTTGGATCTCCTCCTCA+AGG + chr8.3:10849652-10849671 None:intergenic 50.0%
GATTCCTCCCGGTACCAATT+TGG + chr8.3:10849619-10849638 None:intergenic 50.0%
TAGCCCACCCAAATTGGTAC+CGG - chr8.3:10849608-10849627 MS.gene060338:intron 50.0%
! GCGGACCGATTCAATCCAAA+AGG - chr8.3:10849161-10849180 MS.gene060338:intron 50.0%
!!! GGACCTTTTTTCAGCTCCAC+AGG + chr8.3:10848847-10848866 None:intergenic 50.0%
AATGTACGGTCCTAGGCGTG+AGG + chr8.3:10849336-10849355 None:intergenic 55.0%
AGCCCACCCAAATTGGTACC+GGG - chr8.3:10849609-10849628 MS.gene060338:intron 55.0%
ATGTACGGTCCTAGGCGTGA+GGG + chr8.3:10849335-10849354 None:intergenic 55.0%
CACGCCTAGGACCGTACATT+TGG - chr8.3:10849336-10849355 MS.gene060338:intron 55.0%
CGCCCTGTGGAGCTGAAAAA+AGG - chr8.3:10848841-10848860 MS.gene060338:CDS 55.0%
CTCCCGGTACCAATTTGGGT+GGG + chr8.3:10849614-10849633 None:intergenic 55.0%
GAATCGAACCCGAGACCTTG+AGG - chr8.3:10849634-10849653 MS.gene060338:intron 55.0%
GATCTCCTCCTCAAGGTCTC+GGG + chr8.3:10849645-10849664 None:intergenic 55.0%
GGATCTCCTCCTCAAGGTCT+CGG + chr8.3:10849646-10849665 None:intergenic 55.0%
! TTTTCAGCTCCACAGGGCGA+CGG + chr8.3:10848840-10848859 None:intergenic 55.0%
CCTCCCGGTACCAATTTGGG+TGG + chr8.3:10849615-10849634 None:intergenic 60.0%
GCTGGCATCCAGCCAACTTC+TGG - chr8.3:10848998-10849017 MS.gene060338:CDS 60.0%
GTCTCGGGTTCGATTCCTCC+CGG + chr8.3:10849630-10849649 None:intergenic 60.0%
TCGAACCCGAGACCTTGAGG+AGG - chr8.3:10849637-10849656 MS.gene060338:intron 60.0%
TGTACGGTCCTAGGCGTGAG+GGG + chr8.3:10849334-10849353 None:intergenic 60.0%
! CCACCCAAATTGGTACCGGG+AGG - chr8.3:10849612-10849631 MS.gene060338:intron 60.0%
ATGCACACCCCCTCACGCCT+AGG - chr8.3:10849323-10849342 MS.gene060338:intron 65.0%
GTACGGTCCTAGGCGTGAGG+GGG + chr8.3:10849333-10849352 None:intergenic 65.0%
! TCTGACTGGCCGTCGCCCTG+TGG - chr8.3:10848828-10848847 MS.gene060338:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 10848824 10850214 10848824 ID=MS.gene060338
chr8.3 mRNA 10848824 10850214 10848824 ID=MS.gene060338.t1;Parent=MS.gene060338
chr8.3 exon 10850147 10850214 10850147 ID=MS.gene060338.t1.exon1;Parent=MS.gene060338.t1
chr8.3 CDS 10850147 10850214 10850147 ID=cds.MS.gene060338.t1;Parent=MS.gene060338.t1
chr8.3 exon 10848824 10849045 10848824 ID=MS.gene060338.t1.exon2;Parent=MS.gene060338.t1
chr8.3 CDS 10848824 10849045 10848824 ID=cds.MS.gene060338.t1;Parent=MS.gene060338.t1
Gene Sequence

>MS.gene060338

AAATTCTGACTGGCCGTCGCCCTGTGGAGCTGAAAAAAGGTCCTGAAGAGCGAGTTACATTAAGATGGGTAAGCTCACAATTTGTTCTTATTTTGATGTTCTTTACATATATTTGTTAACTATCAATTTAAGCGCTGGCATACTTTGAGAAGATGATATAATATAATTTAATAGGCTGGCATCCAGCCAACTTCTGGTTAAGAATATAATACTTTGAGAAGATGATATAATACTTTGAGCCAAGTGATCCTCTAAGTTAAGAATAATTTCTGCTGTCACTTCGGAAAAATTGATAATTTTGGTCGTTATTCTCAGTGGGAATAATTTGATATGAGTTGCGGACCGATTCAATCCAAAAGGATTTTGTATCAATTGATGAGAGAAAATTGTGATTGGAATAGATCTTGTAAGTTAGGCTCACAAAACTGACATGTAAAGGGATGATTATCCAAAACTTATAATCAAGACTACTTCATATCTATAGTCAATGTGGGACTCTATGCACACCCCCTCACGCCTAGGACCGTACATTTGGAGAATGGACACATATGGGTCGGGTAACAAAAATCTTGATCTAAAAAGATCAATGATAGGCTTTGTTACTATCCTAAAATTTGGGCTTAGGGTCTAACTAAACCCTATAAAATCGATTTTTAAGGGAGGACTATCCAAACCTTATAAGAGATCTAGGATAGACTTTGATACAGTAAAATTTCTCAAACCTGCAAAATTGGTTTAAAATGAGGATTGTCCAAGCCTTTTTTTTTTTGCGAAGAAGCTAAAGTAGCCCACCCAAATTGGTACCGGGAGGAATCGAACCCGAGACCTTGAGGAGGAGATCCAAACCTTATTAGTTACAAGCACTACTTAGGTCATATCTCTAGTAGAAGTGGGGCTTTCAATGTAACTAATTGTAATTTCCCCTTTGAAGAACTGTAGTTGCAACTTGTTCTCTTTGGACGTGTTTACGGGCATTAGCAACATGGGCAAGATTACAGTACTGAAAAGCATTATATATACGCAGAATTTTCTAGTGAACTAGTAATCGTGGGACGGTGTTAATCTTCTTGCTTCTTAGTAATCACTGCAGTAGTAATAGATGAAAAACTTGGTTGTTGTTCCTCTTCATTCTTAAGCACTCACACTATATGATTCTGATCTTTTTGCAGGCCTTCAGGAAATATAATGAAGGAAATGTGGTGGAACTGCTTGATCCTTTAATGCAAGAAGCTGTAAAAACAGATGTTGCTGTGAAGATGTTTGATTTGGCATTTAATTGTGCAGCACCAGTAAGATCAGATAGACCTGACATGAAAACAGTGGGTGAGCAATTGTGGGCAATAAGGGCAGATTACCTCAAGAGTAGTAGCACAACTGCAAGGAGAGAATGA

Protein sequence

>MS.gene060338.t1

KFULAVALWSUKKVLKSELHUDGPSGNIMKEMWWNCLILUCKKLUKQMLLURCLIWHLIVQHQUDQIDLTUKQWVSNCGQUGQITSRVVAQLQGEN