Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26729.t1 | XP_003609926.2 | 85.8 | 113 | 8 | 2 | 1 | 106 | 25 | 136 | 4.80E-43 | 183.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26729.t1 | G7JR56 | 85.8 | 113 | 8 | 2 | 1 | 106 | 25 | 136 | 3.5e-43 | 183.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054468 | MS.gene26729 | 0.815946 | 7.12E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene26729 | 0.810659 | 1.03E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene26729 | 0.801923 | 7.16E-49 | -1.69E-46 |
| MS.gene05973 | MS.gene26729 | 0.803594 | 3.24E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26729.t1 | MTR_4g124450 | 86.207 | 116 | 6 | 4 | 1 | 108 | 25 | 138 | 5.09e-59 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGATATTGGTGGGTTAAA+TGG | 0.242698 | 8.2:-8854451 | MS.gene26729:CDS |
| GTTAAGGTTGATAATGATAT+TGG | 0.344303 | 8.2:-8854463 | MS.gene26729:CDS |
| AACCCTATGGCTTATTCAAT+AGG | 0.387771 | 8.2:-8854679 | None:intergenic |
| GTTAATAGAAGATTTGAGTT+TGG | 0.389742 | 8.2:-8854595 | MS.gene26729:CDS |
| GGAGCAAGGGAATTCTGTTA+AGG | 0.434829 | 8.2:-8854479 | MS.gene26729:CDS |
| TGTTGCTGTTGATGTGGATG+AGG | 0.476033 | 8.2:-8854377 | MS.gene26729:CDS |
| TAACCTATTGAATAAGCCAT+AGG | 0.507567 | 8.2:+8854676 | None:intergenic |
| GTTGCTGTTGATGTGGATGA+GGG | 0.514964 | 8.2:-8854376 | MS.gene26729:CDS |
| TTTGCATCTGATTTGGAGCA+AGG | 0.525020 | 8.2:-8854493 | MS.gene26729:CDS |
| TCCTTGTTCTCATTTGAGCA+AGG | 0.527633 | 8.2:-8854644 | MS.gene26729:CDS |
| GCTTGTTGTTGCTGTTGATG+TGG | 0.530835 | 8.2:-8854383 | MS.gene26729:CDS |
| TTGCATCTGATTTGGAGCAA+GGG | 0.562036 | 8.2:-8854492 | MS.gene26729:CDS |
| AGGTTGATAATGATATTGGT+GGG | 0.577068 | 8.2:-8854459 | MS.gene26729:CDS |
| AAGGTTGATAATGATATTGG+TGG | 0.579097 | 8.2:-8854460 | MS.gene26729:CDS |
| CTAGTGCTAGTAATAGCAGT+AGG | 0.623766 | 8.2:-8854549 | MS.gene26729:CDS |
| ACCTTGCTCAAATGAGAACA+AGG | 0.632511 | 8.2:+8854643 | None:intergenic |
| AAACGTTGATCCCGAAAACG+AGG | 0.653630 | 8.2:+8854415 | None:intergenic |
| AGAGCTAATGCAAGGCCTCG+CGG | 0.678484 | 8.2:-8854517 | MS.gene26729:CDS |
| AAATCAGATGCAAAACCGCG+AGG | 0.732365 | 8.2:+8854502 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGCTAATGTTTTTGATTTT+GGG | - | chr8.2:8854400-8854419 | MS.gene26729:CDS | 20.0% |
| !!! | TATGCTAATGTTTTTGATTT+TGG | - | chr8.2:8854399-8854418 | MS.gene26729:CDS | 20.0% |
| !! | GTTAATAGAAGATTTGAGTT+TGG | - | chr8.2:8854423-8854442 | MS.gene26729:CDS | 25.0% |
| !!! | GTTAAGGTTGATAATGATAT+TGG | - | chr8.2:8854555-8854574 | MS.gene26729:CDS | 25.0% |
| !!! | TGCTAATGTTTTTGATTTTG+GGG | - | chr8.2:8854401-8854420 | MS.gene26729:CDS | 25.0% |
| ! | TTTTTGTAAGAGCTAATGCA+AGG | - | chr8.2:8854493-8854512 | MS.gene26729:CDS | 30.0% |
| !! | AAGGTTGATAATGATATTGG+TGG | - | chr8.2:8854558-8854577 | MS.gene26729:CDS | 30.0% |
| !! | AATGATATTGGTGGGTTAAA+TGG | - | chr8.2:8854567-8854586 | MS.gene26729:CDS | 30.0% |
| !! | AGGTTGATAATGATATTGGT+GGG | - | chr8.2:8854559-8854578 | MS.gene26729:CDS | 30.0% |
| GAGTTTGGTAGAAAAACTTG+TGG | - | chr8.2:8854438-8854457 | MS.gene26729:CDS | 35.0% | |
| ACCTTGCTCAAATGAGAACA+AGG | + | chr8.2:8854378-8854397 | None:intergenic | 40.0% | |
| CTAGTGCTAGTAATAGCAGT+AGG | - | chr8.2:8854469-8854488 | MS.gene26729:CDS | 40.0% | |
| TCCTTGTTCTCATTTGAGCA+AGG | - | chr8.2:8854374-8854393 | MS.gene26729:CDS | 40.0% | |
| !! | TTGCATCTGATTTGGAGCAA+GGG | - | chr8.2:8854526-8854545 | MS.gene26729:CDS | 40.0% |
| !! | TTTGCATCTGATTTGGAGCA+AGG | - | chr8.2:8854525-8854544 | MS.gene26729:CDS | 40.0% |
| AAACGTTGATCCCGAAAACG+AGG | + | chr8.2:8854606-8854625 | None:intergenic | 45.0% | |
| AAATCAGATGCAAAACCGCG+AGG | + | chr8.2:8854519-8854538 | None:intergenic | 45.0% | |
| GGAGCAAGGGAATTCTGTTA+AGG | - | chr8.2:8854539-8854558 | MS.gene26729:CDS | 45.0% | |
| ! | GTTGCTGTTGATGTGGATGA+GGG | - | chr8.2:8854642-8854661 | MS.gene26729:CDS | 45.0% |
| ! | TCGCGGTTTTGCATCTGATT+TGG | - | chr8.2:8854518-8854537 | MS.gene26729:CDS | 45.0% |
| ! | TGTTGCTGTTGATGTGGATG+AGG | - | chr8.2:8854641-8854660 | MS.gene26729:CDS | 45.0% |
| !! | GCTTGTTGTTGCTGTTGATG+TGG | - | chr8.2:8854635-8854654 | MS.gene26729:CDS | 45.0% |
| ! | GTTCTGCTACGCCTCGTTTT+CGG | - | chr8.2:8854592-8854611 | MS.gene26729:CDS | 50.0% |
| ! | TTCTGCTACGCCTCGTTTTC+GGG | - | chr8.2:8854593-8854612 | MS.gene26729:CDS | 50.0% |
| AGAGCTAATGCAAGGCCTCG+CGG | - | chr8.2:8854501-8854520 | MS.gene26729:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 8854345 | 8854695 | 8854345 | ID=MS.gene26729 |
| chr8.2 | mRNA | 8854345 | 8854695 | 8854345 | ID=MS.gene26729.t1;Parent=MS.gene26729 |
| chr8.2 | exon | 8854345 | 8854695 | 8854345 | ID=MS.gene26729.t1.exon1;Parent=MS.gene26729.t1 |
| chr8.2 | CDS | 8854345 | 8854695 | 8854345 | ID=cds.MS.gene26729.t1;Parent=MS.gene26729.t1 |
| Gene Sequence |
| Protein sequence |
