AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26868


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26868.t1 MTR_1g103420 95.089 224 4 1 1 224 1 217 1.50e-143 399
MS.gene26868.t1 MTR_5g006940 56.250 224 86 5 1 224 1 212 4.10e-67 206
MS.gene26868.t1 MTR_1g071720 49.254 134 63 5 26 156 26 157 2.34e-31 114
MS.gene26868.t1 MTR_1g071720 55.660 106 43 4 26 128 26 130 2.91e-31 113
MS.gene26868.t1 MTR_7g095250 48.544 103 46 3 37 132 2 104 8.74e-23 91.3
MS.gene26868.t1 MTR_1g103450 33.636 110 65 4 10 118 8 110 9.08e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26868.t1 AT1G05450 60.993 141 48 3 26 166 27 160 1.54e-46 153
MS.gene26868.t1 AT3G22620 44.690 226 100 8 1 224 1 203 7.43e-43 144
MS.gene26868.t1 AT2G48140 48.428 159 70 6 12 159 7 164 2.26e-36 127
MS.gene26868.t1 AT2G48140 53.913 115 51 2 12 125 7 120 2.19e-35 122

Find 70 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCTCTTCACACCTTGTA+TGG 0.248146 1.4:-80014552 MS.gene26868:CDS
AATCCATGCCACTACTTGTT+AGG 0.277189 1.4:+80014462 None:intergenic
AAGAGAGTGCTTATTGTAGA+AGG 0.288971 1.4:+80014566 None:intergenic
ATAGCTAAGGTTCTATTGAT+TGG 0.350447 1.4:+80014398 None:intergenic
CTTACAGGTAGTAGTGCTAA+TGG 0.358055 1.4:-80014524 MS.gene26868:CDS
GGAGATGAAGTTGGTGAAGC+TGG 0.379930 1.4:+80013710 None:intergenic
GTCATTAATATTGTCATGGC+TGG 0.381229 1.4:-80014623 MS.gene26868:CDS
CCATCGCCCTCGGTGAATTC+CGG 0.382892 1.4:-80013644 MS.gene26868:CDS
CTTCCAGTTGTCGATGTAGA+TGG 0.386358 1.4:+80013617 None:intergenic
TACTGTTCTATTGATTGCAC+TGG 0.398561 1.4:-80013540 MS.gene26868:CDS
TGCTAATAACCTTGAGGACT+AGG 0.423773 1.4:+80013856 None:intergenic
CTTATTGTAGAAGGGTTACA+TGG 0.446986 1.4:+80014575 None:intergenic
TCACCTGCATCTTCTCCCTC+AGG 0.449806 1.4:-80013892 MS.gene26868:CDS
ATCCATGCCACTACTTGTTA+GGG 0.450316 1.4:+80014463 None:intergenic
TGGGGTGTTGATTTGTGCAT+TGG 0.461296 1.4:+80014595 None:intergenic
TGAGACATTGTAAGATGTAA+TGG 0.469321 1.4:+80013565 None:intergenic
GCTAATAACCTTGAGGACTA+GGG 0.472496 1.4:+80013857 None:intergenic
TTCACCAACTTCATCTCCTC+TGG 0.485231 1.4:-80013705 MS.gene26868:CDS
CCTCGTGCTTGTAACATGCC+TGG 0.487865 1.4:-80014368 MS.gene26868:CDS
ATTCCATCTACATCGACAAC+TGG 0.490256 1.4:-80013620 MS.gene26868:CDS
ATGTCACCTAGCAATAATAT+TGG 0.492160 1.4:-80014645 MS.gene26868:CDS
GCTTCACCACTTCCTGCTCC+TGG 0.495227 1.4:-80014195 MS.gene26868:intron
TTTGACTCCACCATCGCCCT+CGG 0.501156 1.4:-80013654 MS.gene26868:CDS
GAGATGAAGTTGGTGAAGCT+GGG 0.504474 1.4:+80013711 None:intergenic
GCATTACCAGGAGCAGGAAG+TGG 0.522233 1.4:+80014189 None:intergenic
AGAGAGTGCTTATTGTAGAA+GGG 0.525567 1.4:+80014567 None:intergenic
TAATGACCAATATTATTGCT+AGG 0.532925 1.4:+80014639 None:intergenic
AATCCTGAGGGAGAAGATGC+AGG 0.533311 1.4:+80013889 None:intergenic
AGCAGGAAGTGGTGAAGCTG+AGG 0.535400 1.4:+80014200 None:intergenic
ATTCCAACCCCTAGTCCTCA+AGG 0.540237 1.4:-80013865 MS.gene26868:intron
ACTCTCTTCACACCTTGTAT+GGG 0.540459 1.4:-80014551 MS.gene26868:CDS
AATTTGGCATTACCAGGAGC+AGG 0.546347 1.4:+80014183 None:intergenic
TTCACAGGTCCTGTGGCTCT+AGG 0.546401 1.4:-80013925 MS.gene26868:intron
TAACCTTGAGGACTAGGGGT+TGG 0.546635 1.4:+80013862 None:intergenic
ATGTATATTCACAGGTCCTG+TGG 0.548141 1.4:-80013932 MS.gene26868:intron
ATCCCTAACAAGTAGTGGCA+TGG 0.551127 1.4:-80014465 MS.gene26868:CDS
CTTAAATCCCTAACAAGTAG+TGG 0.551269 1.4:-80014470 MS.gene26868:CDS
ATTGGTCATTAATATTGTCA+TGG 0.553363 1.4:-80014627 MS.gene26868:CDS
TGTCGATGTAGATGGAATAC+CGG 0.553646 1.4:+80013625 None:intergenic
TGAGATGGTCCTAGAGCCAC+AGG 0.556902 1.4:+80013916 None:intergenic
GAATTCACCGAGGGCGATGG+TGG 0.560540 1.4:+80013647 None:intergenic
CCGGAATTCACCGAGGGCGA+TGG 0.561975 1.4:+80013644 None:intergenic
GGCGATGGTGGAGTCAAAAG+AGG 0.563454 1.4:+80013659 None:intergenic
ACTGTTCTATTGATTGCACT+GGG 0.566423 1.4:-80013539 MS.gene26868:CDS
CCTGTAAGAAAACCCATACA+AGG 0.567820 1.4:+80014539 None:intergenic
GGAACATTTGTGTCTTGTTG+TGG 0.568188 1.4:+80013680 None:intergenic
TAAGATGTAATGGCAGATGA+TGG 0.568483 1.4:+80013575 None:intergenic
TTGCATTGAACTGGAACACC+AGG 0.568933 1.4:+80014350 None:intergenic
AAGAGCACCGCAACATTCAG+TGG 0.581316 1.4:+80014490 None:intergenic
AGGGAACACTAGCAGTTACA+AGG 0.586483 1.4:+80014429 None:intergenic
AGTGCAATCAATAGAACAGT+AGG 0.586789 1.4:+80013542 None:intergenic
GTGTCTTGTTGTGGTGCCAG+AGG 0.590812 1.4:+80013689 None:intergenic
GCACCGCAACATTCAGTGGT+TGG 0.593515 1.4:+80014494 None:intergenic
TCTACATCGACAACTGGAAG+CGG 0.603015 1.4:-80013614 MS.gene26868:CDS
GAAGATGCAGGTGAATGAGA+TGG 0.607870 1.4:+80013901 None:intergenic
GGTGTTCCAGTTCAATGCAA+AGG 0.630148 1.4:-80014347 MS.gene26868:intron
CCAGGCATGTTACAAGCACG+AGG 0.631598 1.4:+80014368 None:intergenic
ACGAGGGAGAGAGATAGCTA+AGG 0.635982 1.4:+80014385 None:intergenic
CTAATAACCTTGAGGACTAG+GGG 0.642081 1.4:+80013858 None:intergenic
TCACCAACCACTGAATGTTG+CGG 0.642405 1.4:-80014497 MS.gene26868:CDS
TTATTGTAGAAGGGTTACAT+GGG 0.644859 1.4:+80014576 None:intergenic
GCAACATTCAGTGGTTGGTG+AGG 0.651267 1.4:+80014499 None:intergenic
GGGGTTGGAATAAATCCTGA+GGG 0.662198 1.4:+80013877 None:intergenic
AGGGGTTGGAATAAATCCTG+AGG 0.672890 1.4:+80013876 None:intergenic
GGTGCCAGAGGAGATGAAGT+TGG 0.674504 1.4:+80013701 None:intergenic
GGAATACCGGAATTCACCGA+GGG 0.686162 1.4:+80013638 None:intergenic
TATTGTAGAAGGGTTACATG+GGG 0.692481 1.4:+80014577 None:intergenic
TGGTGAAGCTGGGATGTCAG+AGG 0.694095 1.4:+80013721 None:intergenic
TGGAATACCGGAATTCACCG+AGG 0.697163 1.4:+80013637 None:intergenic
CAGGCATGTTACAAGCACGA+GGG 0.748196 1.4:+80014369 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATTGAAATTGAAATT+TGG + chr1.4:80014015-80014034 None:intergenic 10.0%
!! CATTATTAAGATTAATTCAA+AGG + chr1.4:80014221-80014240 None:intergenic 15.0%
!!! GTTAGAAAATATTTTTGTTA+GGG + chr1.4:80014073-80014092 None:intergenic 15.0%
!!! TGTTAGAAAATATTTTTGTT+AGG + chr1.4:80014074-80014093 None:intergenic 15.0%
! ATTGGTCATTAATATTGTCA+TGG - chr1.4:80013552-80013571 MS.gene26868:CDS 25.0%
! TAATGACCAATATTATTGCT+AGG + chr1.4:80013543-80013562 None:intergenic 25.0%
! TCAAAGAAAAATGTAAGAGT+TGG + chr1.4:80014431-80014450 None:intergenic 25.0%
! TCAAAGGAAAAATAGTTTAG+AGG + chr1.4:80014205-80014224 None:intergenic 25.0%
!! TTTTGTTAGGGATTATAGAA+GGG + chr1.4:80014061-80014080 None:intergenic 25.0%
!! TTTTTGTTAGGGATTATAGA+AGG + chr1.4:80014062-80014081 None:intergenic 25.0%
!!! CTATTGATTGGAATTTTGAA+GGG + chr1.4:80013772-80013791 None:intergenic 25.0%
!!! TCTATTGATTGGAATTTTGA+AGG + chr1.4:80013773-80013792 None:intergenic 25.0%
!!! TGATTTTGATGTATATTCAC+AGG - chr1.4:80014239-80014258 MS.gene26868:intron 25.0%
AATTGAAATTTGGCATTACC+AGG + chr1.4:80014005-80014024 None:intergenic 30.0%
ATAGCTAAGGTTCTATTGAT+TGG + chr1.4:80013784-80013803 None:intergenic 30.0%
ATGTCACCTAGCAATAATAT+TGG - chr1.4:80013534-80013553 MS.gene26868:CDS 30.0%
CTATCATCTAGTAACATTGT+AGG - chr1.4:80014172-80014191 MS.gene26868:intron 30.0%
TGAGACATTGTAAGATGTAA+TGG + chr1.4:80014617-80014636 None:intergenic 30.0%
TTATTGTAGAAGGGTTACAT+GGG + chr1.4:80013606-80013625 None:intergenic 30.0%
! TTTTTACCTTTGCATTGAAC+TGG + chr1.4:80013841-80013860 None:intergenic 30.0%
AGTGCAATCAATAGAACAGT+AGG + chr1.4:80014640-80014659 None:intergenic 35.0%
CTTAAATCCCTAACAAGTAG+TGG - chr1.4:80013709-80013728 MS.gene26868:CDS 35.0%
CTTATTGTAGAAGGGTTACA+TGG + chr1.4:80013607-80013626 None:intergenic 35.0%
GTCATTAATATTGTCATGGC+TGG - chr1.4:80013556-80013575 MS.gene26868:CDS 35.0%
GTGTAATGCTAATAACCTTG+AGG + chr1.4:80014332-80014351 None:intergenic 35.0%
TAAGATGTAATGGCAGATGA+TGG + chr1.4:80014607-80014626 None:intergenic 35.0%
TATTGTAGAAGGGTTACATG+GGG + chr1.4:80013605-80013624 None:intergenic 35.0%
! ACTGTTCTATTGATTGCACT+GGG - chr1.4:80014640-80014659 MS.gene26868:CDS 35.0%
! TACTGTTCTATTGATTGCAC+TGG - chr1.4:80014639-80014658 MS.gene26868:CDS 35.0%
!! AAGAGAGTGCTTATTGTAGA+AGG + chr1.4:80013616-80013635 None:intergenic 35.0%
!! AGAGAGTGCTTATTGTAGAA+GGG + chr1.4:80013615-80013634 None:intergenic 35.0%
AATCCATGCCACTACTTGTT+AGG + chr1.4:80013720-80013739 None:intergenic 40.0%
ACTCTCTTCACACCTTGTAT+GGG - chr1.4:80013628-80013647 MS.gene26868:CDS 40.0%
ATGTATATTCACAGGTCCTG+TGG - chr1.4:80014247-80014266 MS.gene26868:intron 40.0%
ATTCCATCTACATCGACAAC+TGG - chr1.4:80014559-80014578 MS.gene26868:CDS 40.0%
CCTGTAAGAAAACCCATACA+AGG + chr1.4:80013643-80013662 None:intergenic 40.0%
CTAATAACCTTGAGGACTAG+GGG + chr1.4:80014324-80014343 None:intergenic 40.0%
CTTACAGGTAGTAGTGCTAA+TGG - chr1.4:80013655-80013674 MS.gene26868:CDS 40.0%
GCTAATAACCTTGAGGACTA+GGG + chr1.4:80014325-80014344 None:intergenic 40.0%
TGCTAATAACCTTGAGGACT+AGG + chr1.4:80014326-80014345 None:intergenic 40.0%
TGTCGATGTAGATGGAATAC+CGG + chr1.4:80014557-80014576 None:intergenic 40.0%
! ATCCATGCCACTACTTGTTA+GGG + chr1.4:80013719-80013738 None:intergenic 40.0%
! CCTTGTATGGGTTTTCTTAC+AGG - chr1.4:80013640-80013659 MS.gene26868:CDS 40.0%
! GGAACATTTGTGTCTTGTTG+TGG + chr1.4:80014502-80014521 None:intergenic 40.0%
AATTTGGCATTACCAGGAGC+AGG + chr1.4:80013999-80014018 None:intergenic 45.0%
AGGGAACACTAGCAGTTACA+AGG + chr1.4:80013753-80013772 None:intergenic 45.0%
AGGGGTTGGAATAAATCCTG+AGG + chr1.4:80014306-80014325 None:intergenic 45.0%
ATCCCTAACAAGTAGTGGCA+TGG - chr1.4:80013714-80013733 MS.gene26868:CDS 45.0%
CACTCTCTTCACACCTTGTA+TGG - chr1.4:80013627-80013646 MS.gene26868:CDS 45.0%
CTTCCAGTTGTCGATGTAGA+TGG + chr1.4:80014565-80014584 None:intergenic 45.0%
GAAGATGCAGGTGAATGAGA+TGG + chr1.4:80014281-80014300 None:intergenic 45.0%
GGGGTTGGAATAAATCCTGA+GGG + chr1.4:80014305-80014324 None:intergenic 45.0%
GGTGTTCCAGTTCAATGCAA+AGG - chr1.4:80013832-80013851 MS.gene26868:intron 45.0%
TCACCAACCACTGAATGTTG+CGG - chr1.4:80013682-80013701 MS.gene26868:CDS 45.0%
TCTACATCGACAACTGGAAG+CGG - chr1.4:80014565-80014584 MS.gene26868:CDS 45.0%
TTCACCAACTTCATCTCCTC+TGG - chr1.4:80014474-80014493 MS.gene26868:CDS 45.0%
TTGCATTGAACTGGAACACC+AGG + chr1.4:80013832-80013851 None:intergenic 45.0%
!! GAGATGAAGTTGGTGAAGCT+GGG + chr1.4:80014471-80014490 None:intergenic 45.0%
!! TGGGGTGTTGATTTGTGCAT+TGG + chr1.4:80013587-80013606 None:intergenic 45.0%
AATCCTGAGGGAGAAGATGC+AGG + chr1.4:80014293-80014312 None:intergenic 50.0%
ACGAGGGAGAGAGATAGCTA+AGG + chr1.4:80013797-80013816 None:intergenic 50.0%
ATTCCAACCCCTAGTCCTCA+AGG - chr1.4:80014314-80014333 MS.gene26868:intron 50.0%
CAGGCATGTTACAAGCACGA+GGG + chr1.4:80013813-80013832 None:intergenic 50.0%
GGAATACCGGAATTCACCGA+GGG + chr1.4:80014544-80014563 None:intergenic 50.0%
TAACCTTGAGGACTAGGGGT+TGG + chr1.4:80014320-80014339 None:intergenic 50.0%
TGGAATACCGGAATTCACCG+AGG + chr1.4:80014545-80014564 None:intergenic 50.0%
! GCAACATTCAGTGGTTGGTG+AGG + chr1.4:80013683-80013702 None:intergenic 50.0%
!! AAGAGCACCGCAACATTCAG+TGG + chr1.4:80013692-80013711 None:intergenic 50.0%
!! GGAGATGAAGTTGGTGAAGC+TGG + chr1.4:80014472-80014491 None:intergenic 50.0%
AGCAGGAAGTGGTGAAGCTG+AGG + chr1.4:80013982-80014001 None:intergenic 55.0%
CCAGGCATGTTACAAGCACG+AGG + chr1.4:80013814-80013833 None:intergenic 55.0%
CCTCGTGCTTGTAACATGCC+TGG - chr1.4:80013811-80013830 MS.gene26868:intron 55.0%
GCACCGCAACATTCAGTGGT+TGG + chr1.4:80013688-80013707 None:intergenic 55.0%
GCATTACCAGGAGCAGGAAG+TGG + chr1.4:80013993-80014012 None:intergenic 55.0%
GGTGCCAGAGGAGATGAAGT+TGG + chr1.4:80014481-80014500 None:intergenic 55.0%
TCACCTGCATCTTCTCCCTC+AGG - chr1.4:80014287-80014306 MS.gene26868:intron 55.0%
TGAGATGGTCCTAGAGCCAC+AGG + chr1.4:80014266-80014285 None:intergenic 55.0%
TGGTGAAGCTGGGATGTCAG+AGG + chr1.4:80014461-80014480 None:intergenic 55.0%
TTCACAGGTCCTGTGGCTCT+AGG - chr1.4:80014254-80014273 MS.gene26868:intron 55.0%
TTTGACTCCACCATCGCCCT+CGG - chr1.4:80014525-80014544 MS.gene26868:CDS 55.0%
! GTGTCTTGTTGTGGTGCCAG+AGG + chr1.4:80014493-80014512 None:intergenic 55.0%
!! GGCGATGGTGGAGTCAAAAG+AGG + chr1.4:80014523-80014542 None:intergenic 55.0%
CCATCGCCCTCGGTGAATTC+CGG - chr1.4:80014535-80014554 MS.gene26868:CDS 60.0%
GAATTCACCGAGGGCGATGG+TGG + chr1.4:80014535-80014554 None:intergenic 60.0%
GCTTCACCACTTCCTGCTCC+TGG - chr1.4:80013984-80014003 MS.gene26868:intron 60.0%
CCGGAATTCACCGAGGGCGA+TGG + chr1.4:80014538-80014557 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 80013514 80014687 80013514 ID=MS.gene26868
chr1.4 mRNA 80013514 80014687 80013514 ID=MS.gene26868.t1;Parent=MS.gene26868
chr1.4 exon 80014348 80014687 80014348 ID=MS.gene26868.t1.exon1;Parent=MS.gene26868.t1
chr1.4 CDS 80014348 80014687 80014348 ID=cds.MS.gene26868.t1;Parent=MS.gene26868.t1
chr1.4 exon 80014196 80014222 80014196 ID=MS.gene26868.t1.exon2;Parent=MS.gene26868.t1
chr1.4 CDS 80014196 80014222 80014196 ID=cds.MS.gene26868.t1;Parent=MS.gene26868.t1
chr1.4 exon 80013866 80013940 80013866 ID=MS.gene26868.t1.exon3;Parent=MS.gene26868.t1
chr1.4 CDS 80013866 80013940 80013866 ID=cds.MS.gene26868.t1;Parent=MS.gene26868.t1
chr1.4 exon 80013514 80013746 80013514 ID=MS.gene26868.t1.exon4;Parent=MS.gene26868.t1
chr1.4 CDS 80013514 80013746 80013514 ID=cds.MS.gene26868.t1;Parent=MS.gene26868.t1
Gene Sequence

>MS.gene26868

ATGGCAAATTATGTTGCTCTATGTCACCTAGCAATAATATTGGTCATTAATATTGTCATGGCTGGACAAGCCAATGCACAAATCAACACCCCATGTAACCCTTCTACAATAAGCACTCTCTTCACACCTTGTATGGGTTTTCTTACAGGTAGTAGTGCTAATGGCACCTCACCAACCACTGAATGTTGCGGTGCTCTTAAATCCCTAACAAGTAGTGGCATGGATTGTTTGTGTCTCCTTGTAACTGCTAGTGTTCCCTTCAAAATTCCAATCAATAGAACCTTAGCTATCTCTCTCCCTCGTGCTTGTAACATGCCTGGTGTTCCAGTTCAATGCAAAGGTAAAAAAAAAAAAAAAACTACAATATTTTTCTTGTGTTTGTAACTTTGTAGAGTAAAATTTGTTATTCTCACTACAATAATAAAGCTAAGATGTTACTTTTTTTTTTTTTTTTTTACTTTGCAGCCTCAGCTTCACCACTTCCTGCTCCTGGTAATGCCAAATTTCAATTTCAATAATTTTTGTACACAAATTCTTACATAAACCCTTCTATAATCCCTAACAAAAATATTTTCTAACAAACTCTTCGCGACATGTGAATTTCATTTCTTTACTTAGAAATTGAGTCATGCTAAATAAGAAATGAAACTAGCATGATCTATCATCTAGTAACATTGTAGGTTTAATTCCTCTAAACTATTTTTCCTTTGAATTAATCTTAATAATGATTTTGATGTATATTCACAGGTCCTGTGGCTCTAGGACCATCTCATTCACCTGCATCTTCTCCCTCAGGATTTATTCCAACCCCTAGTCCTCAAGGTTATTAGCATTACACTATTCTATTACAATACAAGCTTATACAAGTTTAAACTTTTCATAGTGCTAAGAGCTTCTTAATGTGTTACTAACATCCAACTCTTACATTTTTCTTTGAGCAGCATCCTCTGACATCCCAGCTTCACCAACTTCATCTCCTCTGGCACCACAACAAGACACAAATGTTCCTCTTTTGACTCCACCATCGCCCTCGGTGAATTCCGGTATTCCATCTACATCGACAACTGGAAGCGGTCGCTCTTCTCTGACTCCATCATCTGCCATTACATCTTACAATGTCTCACCTACTGTTCTATTGATTGCACTGGGATTTGTTGCTCTCAAATACTACTAG

Protein sequence

>MS.gene26868.t1

MANYVALCHLAIILVINIVMAGQANAQINTPCNPSTISTLFTPCMGFLTGSSANGTSPTTECCGALKSLTSSGMDCLCLLVTASVPFKIPINRTLAISLPRACNMPGVPVQCKASASPLPAPGPVALGPSHSPASSPSGFIPTPSPQASSDIPASPTSSPLAPQQDTNVPLLTPPSPSVNSGIPSTSTTGSGRSSLTPSSAITSYNVSPTVLLIALGFVALKYY