Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053289.t1 | XP_013469813.1 | 74.1 | 205 | 46 | 1 | 1 | 205 | 20 | 217 | 1.30E-48 | 203 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053289.t1 | A0A072VQE2 | 74.1 | 205 | 46 | 1 | 1 | 205 | 20 | 217 | 9.7e-49 | 203.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053287 | MS.gene053289 | 0.999853 | 0.00E+00 | -1.69E-46 |
| MS.gene053288 | MS.gene053289 | 0.990478 | 7.96E-183 | -1.69E-46 |
| MS.gene053289 | MS.gene26868 | 0.931346 | 4.33E-94 | -1.69E-46 |
| MS.gene053289 | MS.gene93545 | 0.814422 | 1.55E-51 | -1.69E-46 |
| MS.gene053289 | MS.gene93574 | 0.821381 | 4.16E-53 | -1.69E-46 |
| MS.gene053289 | MS.gene95784 | 0.91926 | 5.62E-87 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053289.t1 | MTR_1g103420 | 95.122 | 205 | 3 | 1 | 1 | 205 | 20 | 217 | 4.24e-130 | 365 |
| MS.gene053289.t1 | MTR_5g006940 | 59.512 | 205 | 71 | 5 | 1 | 205 | 20 | 212 | 5.20e-63 | 194 |
| MS.gene053289.t1 | MTR_1g071720 | 56.190 | 105 | 42 | 4 | 8 | 109 | 27 | 130 | 1.53e-31 | 113 |
| MS.gene053289.t1 | MTR_1g071720 | 56.190 | 105 | 42 | 4 | 8 | 109 | 27 | 130 | 1.68e-31 | 113 |
| MS.gene053289.t1 | MTR_7g095250 | 48.544 | 103 | 46 | 3 | 18 | 113 | 2 | 104 | 7.93e-23 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053289.t1 | AT1G05450 | 60.993 | 141 | 48 | 3 | 7 | 147 | 27 | 160 | 2.39e-46 | 152 |
| MS.gene053289.t1 | AT3G22620 | 47.692 | 195 | 80 | 6 | 13 | 205 | 29 | 203 | 2.62e-41 | 139 |
| MS.gene053289.t1 | AT2G48140 | 50.331 | 151 | 63 | 6 | 1 | 140 | 15 | 164 | 5.44e-35 | 122 |
| MS.gene053289.t1 | AT2G48140 | 57.009 | 107 | 44 | 2 | 1 | 106 | 15 | 120 | 2.56e-34 | 119 |
Find 0 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTATTGAAATTGAAATT+TGG | - | 10826:5658-5677 | None:intergenic | 10.0% |
| !! | CATTATTAAGATTAATTCAA+AGG | - | 10826:5864-5883 | None:intergenic | 15.0% |
| !!! | GTTAGAAAATATTTTTGTTA+GGG | - | 10826:5716-5735 | None:intergenic | 15.0% |
| !!! | TGTTAGAAAATATTTTTGTT+AGG | - | 10826:5717-5736 | None:intergenic | 15.0% |
| ! | TCAAAGAAAAATGTAAGAGT+TGG | - | 10826:6074-6093 | None:intergenic | 25.0% |
| ! | TCAAAGGAAAAATAGTTTAG+AGG | - | 10826:5848-5867 | None:intergenic | 25.0% |
| !! | TTTTGTTAGGGATTATAGAA+GGG | - | 10826:5704-5723 | None:intergenic | 25.0% |
| !! | TTTTTGTTAGGGATTATAGA+AGG | - | 10826:5705-5724 | None:intergenic | 25.0% |
| !!! | CTATTGATTGGAATTTTGAA+GGG | - | 10826:5417-5436 | None:intergenic | 25.0% |
| !!! | TCTATTGATTGGAATTTTGA+AGG | - | 10826:5418-5437 | None:intergenic | 25.0% |
| !!! | TGATTTTGATGTATATTCAC+AGG | + | 10826:5882-5901 | MS.gene053289:intron | 25.0% |
| AATTGAAATTTGGCATTACC+AGG | - | 10826:5648-5667 | None:intergenic | 30.0% | |
| ATAGCTAAGGTTCTATTGAT+TGG | - | 10826:5429-5448 | None:intergenic | 30.0% | |
| CTATCATCTAGTAACATTGT+AGG | + | 10826:5815-5834 | MS.gene053289:intron | 30.0% | |
| TGAGACATTGTAAGATGTAA+TGG | - | 10826:6260-6279 | None:intergenic | 30.0% | |
| TTATTGTAGAAGGGTTACAT+GGG | - | 10826:5251-5270 | None:intergenic | 30.0% | |
| ! | TTTTTACCTTTGCATTGAAC+TGG | - | 10826:5486-5505 | None:intergenic | 30.0% |
| AGTGCAATCAATAGAACAGT+AGG | - | 10826:6283-6302 | None:intergenic | 35.0% | |
| CTTAAATCCCTAACAAGTAG+TGG | + | 10826:5354-5373 | MS.gene053289:CDS | 35.0% | |
| CTTATTGTAGAAGGGTTACA+TGG | - | 10826:5252-5271 | None:intergenic | 35.0% | |
| GTGTAATGCTAATAACCTTG+AGG | - | 10826:5975-5994 | None:intergenic | 35.0% | |
| TAAGATGTAATGGCAGATGA+TGG | - | 10826:6250-6269 | None:intergenic | 35.0% | |
| TATTGTAGAAGGGTTACATG+GGG | - | 10826:5250-5269 | None:intergenic | 35.0% | |
| ! | ACTGTTCTATTGATTGCACT+GGG | + | 10826:6283-6302 | MS.gene053289:CDS | 35.0% |
| ! | TACTGTTCTATTGATTGCAC+TGG | + | 10826:6282-6301 | MS.gene053289:CDS | 35.0% |
| !! | AAGAGAGTGCTTATTGTAGA+AGG | - | 10826:5261-5280 | None:intergenic | 35.0% |
| !! | AGAGAGTGCTTATTGTAGAA+GGG | - | 10826:5260-5279 | None:intergenic | 35.0% |
| AATCCATGCCACTACTTGTT+AGG | - | 10826:5365-5384 | None:intergenic | 40.0% | |
| ACTCTCTTCACACCTTGTAT+GGG | + | 10826:5273-5292 | MS.gene053289:CDS | 40.0% | |
| ATGTATATTCACAGGTCCTG+TGG | + | 10826:5890-5909 | MS.gene053289:intron | 40.0% | |
| ATTCCATCTACATCGACAAC+TGG | + | 10826:6202-6221 | MS.gene053289:CDS | 40.0% | |
| CCTGTAAGAAAACCCATACA+AGG | - | 10826:5288-5307 | None:intergenic | 40.0% | |
| CTAATAACCTTGAGGACTAG+GGG | - | 10826:5967-5986 | None:intergenic | 40.0% | |
| CTTACAGGTAGTAGTGCTAA+TGG | + | 10826:5300-5319 | MS.gene053289:CDS | 40.0% | |
| GCTAATAACCTTGAGGACTA+GGG | - | 10826:5968-5987 | None:intergenic | 40.0% | |
| TGCTAATAACCTTGAGGACT+AGG | - | 10826:5969-5988 | None:intergenic | 40.0% | |
| TGTCGATGTAGATGGAATAC+CGG | - | 10826:6200-6219 | None:intergenic | 40.0% | |
| ! | ATCCATGCCACTACTTGTTA+GGG | - | 10826:5364-5383 | None:intergenic | 40.0% |
| ! | CCTTGTATGGGTTTTCTTAC+AGG | + | 10826:5285-5304 | MS.gene053289:CDS | 40.0% |
| ! | GGAACATTTGTGTCTTGTTG+TGG | - | 10826:6145-6164 | None:intergenic | 40.0% |
| AATTTGGCATTACCAGGAGC+AGG | - | 10826:5642-5661 | None:intergenic | 45.0% | |
| AGGGAACACTAGCAGTTACA+AGG | - | 10826:5398-5417 | None:intergenic | 45.0% | |
| AGGGGTTGGAATAAATCCTG+AGG | - | 10826:5949-5968 | None:intergenic | 45.0% | |
| ATCCCTAACAAGTAGTGGCA+TGG | + | 10826:5359-5378 | MS.gene053289:CDS | 45.0% | |
| CACTCTCTTCACACCTTGTA+TGG | + | 10826:5272-5291 | MS.gene053289:CDS | 45.0% | |
| CTTCCAGTTGTCGATGTAGA+TGG | - | 10826:6208-6227 | None:intergenic | 45.0% | |
| GAAGATGCAGGTGAATGAGA+TGG | - | 10826:5924-5943 | None:intergenic | 45.0% | |
| GGGGTTGGAATAAATCCTGA+GGG | - | 10826:5948-5967 | None:intergenic | 45.0% | |
| GGTGTTCCAGTTCAATGCAA+AGG | + | 10826:5477-5496 | MS.gene053289:CDS | 45.0% | |
| TCACCAACCACTGAATGTTG+CGG | + | 10826:5327-5346 | MS.gene053289:CDS | 45.0% | |
| TCTACATCGACAACTGGAAG+CGG | + | 10826:6208-6227 | MS.gene053289:CDS | 45.0% | |
| TTCACCAACTTCATCTCCTC+TGG | + | 10826:6117-6136 | MS.gene053289:CDS | 45.0% | |
| TTGCATTGAACTGGAACACC+AGG | - | 10826:5477-5496 | None:intergenic | 45.0% | |
| !! | GAGATGAAGTTGGTGAAGCT+GGG | - | 10826:6114-6133 | None:intergenic | 45.0% |
| !! | TGGGGTGTTGATTTGTGCAT+TGG | - | 10826:5232-5251 | None:intergenic | 45.0% |
| AATCCTGAGGGAGAAGATGC+AGG | - | 10826:5936-5955 | None:intergenic | 50.0% | |
| ACGAGGGAGAGAGATAGCTA+AGG | - | 10826:5442-5461 | None:intergenic | 50.0% | |
| ATTCCAACCCCTAGTCCTCA+AGG | + | 10826:5957-5976 | MS.gene053289:CDS | 50.0% | |
| CAGGCATGTTACAAGCACGA+GGG | - | 10826:5458-5477 | None:intergenic | 50.0% | |
| GGAATACCGGAATTCACCGA+CGG | - | 10826:6187-6206 | None:intergenic | 50.0% | |
| TAACCTTGAGGACTAGGGGT+TGG | - | 10826:5963-5982 | None:intergenic | 50.0% | |
| ! | GCAACATTCAGTGGTTGGTG+AGG | - | 10826:5328-5347 | None:intergenic | 50.0% |
| !! | AAGAGCACCGCAACATTCAG+TGG | - | 10826:5337-5356 | None:intergenic | 50.0% |
| !! | GGAGATGAAGTTGGTGAAGC+TGG | - | 10826:6115-6134 | None:intergenic | 50.0% |
| AGCAGGAAGTGGTGAAGCTG+AGG | - | 10826:5625-5644 | None:intergenic | 55.0% | |
| CCAGGCATGTTACAAGCACG+AGG | - | 10826:5459-5478 | None:intergenic | 55.0% | |
| CCTCGTGCTTGTAACATGCC+TGG | + | 10826:5456-5475 | MS.gene053289:CDS | 55.0% | |
| GCACCGCAACATTCAGTGGT+TGG | - | 10826:5333-5352 | None:intergenic | 55.0% | |
| GCATTACCAGGAGCAGGAAG+TGG | - | 10826:5636-5655 | None:intergenic | 55.0% | |
| GGTGCCAGAGGAGATGAAGT+TGG | - | 10826:6124-6143 | None:intergenic | 55.0% | |
| TCACCTGCATCTTCTCCCTC+AGG | + | 10826:5930-5949 | MS.gene053289:CDS | 55.0% | |
| TGAGATGGTCCTAGAGCCAC+AGG | - | 10826:5909-5928 | None:intergenic | 55.0% | |
| TGGTGAAGCTGGGAGATCAG+AGG | - | 10826:6104-6123 | None:intergenic | 55.0% | |
| TTCACAGGTCCTGTGGCTCT+AGG | + | 10826:5897-5916 | MS.gene053289:intron | 55.0% | |
| ! | GTGTCTTGTTGTGGTGCCAG+AGG | - | 10826:6136-6155 | None:intergenic | 55.0% |
| !! | GGCGATGGCGGAGTCAAAAT+AGG | - | 10826:6166-6185 | None:intergenic | 55.0% |
| CCATCGCCGTCGGTGAATTC+CGG | + | 10826:6178-6197 | MS.gene053289:CDS | 60.0% | |
| GAATTCACCGACGGCGATGG+CGG | - | 10826:6178-6197 | None:intergenic | 60.0% | |
| GCTTCACCACTTCCTGCTCC+TGG | + | 10826:5627-5646 | MS.gene053289:CDS | 60.0% | |
| TTTGACTCCGCCATCGCCGT+CGG | + | 10826:6168-6187 | MS.gene053289:CDS | 60.0% | |
| CCGGAATTCACCGACGGCGA+TGG | - | 10826:6181-6200 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10826 | gene | 5216 | 6330 | 5216 | ID=MS.gene053289 |
| 10826 | mRNA | 5216 | 6330 | 5216 | ID=MS.gene053289.t1;Parent=MS.gene053289 |
| 10826 | exon | 5216 | 5498 | 5216 | ID=MS.gene053289.t1.exon1;Parent=MS.gene053289.t1 |
| 10826 | CDS | 5216 | 5498 | 5216 | ID=cds.MS.gene053289.t1;Parent=MS.gene053289.t1 |
| 10826 | exon | 5622 | 5648 | 5622 | ID=MS.gene053289.t1.exon2;Parent=MS.gene053289.t1 |
| 10826 | CDS | 5622 | 5648 | 5622 | ID=cds.MS.gene053289.t1;Parent=MS.gene053289.t1 |
| 10826 | exon | 5904 | 5978 | 5904 | ID=MS.gene053289.t1.exon3;Parent=MS.gene053289.t1 |
| 10826 | CDS | 5904 | 5978 | 5904 | ID=cds.MS.gene053289.t1;Parent=MS.gene053289.t1 |
| 10826 | exon | 6098 | 6330 | 6098 | ID=MS.gene053289.t1.exon4;Parent=MS.gene053289.t1 |
| 10826 | CDS | 6098 | 6330 | 6098 | ID=cds.MS.gene053289.t1;Parent=MS.gene053289.t1 |
| Gene Sequence |
| Protein sequence |
