Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053287.t1 | XP_013469813.1 | 74.1 | 205 | 46 | 1 | 1 | 205 | 20 | 217 | 1.30E-48 | 203 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053287.t1 | A0A072VQE2 | 74.1 | 205 | 46 | 1 | 1 | 205 | 20 | 217 | 9.7e-49 | 203.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053287 | MS.gene053288 | 0.990511 | 5.54E-183 | -1.69E-46 |
| MS.gene053287 | MS.gene053289 | 0.999853 | 0.00E+00 | -1.69E-46 |
| MS.gene053287 | MS.gene26868 | 0.93113 | 5.95E-94 | -1.69E-46 |
| MS.gene053287 | MS.gene93545 | 0.814477 | 1.51E-51 | -1.69E-46 |
| MS.gene053287 | MS.gene93574 | 0.821475 | 3.96E-53 | -1.69E-46 |
| MS.gene053287 | MS.gene95784 | 0.919054 | 7.26E-87 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053287.t1 | MTR_1g103420 | 95.122 | 205 | 3 | 1 | 1 | 205 | 20 | 217 | 4.24e-130 | 365 |
| MS.gene053287.t1 | MTR_5g006940 | 59.512 | 205 | 71 | 5 | 1 | 205 | 20 | 212 | 5.20e-63 | 194 |
| MS.gene053287.t1 | MTR_1g071720 | 56.190 | 105 | 42 | 4 | 8 | 109 | 27 | 130 | 1.53e-31 | 113 |
| MS.gene053287.t1 | MTR_1g071720 | 56.190 | 105 | 42 | 4 | 8 | 109 | 27 | 130 | 1.68e-31 | 113 |
| MS.gene053287.t1 | MTR_7g095250 | 48.544 | 103 | 46 | 3 | 18 | 113 | 2 | 104 | 7.93e-23 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053287.t1 | AT1G05450 | 60.993 | 141 | 48 | 3 | 7 | 147 | 27 | 160 | 2.39e-46 | 152 |
| MS.gene053287.t1 | AT3G22620 | 47.692 | 195 | 80 | 6 | 13 | 205 | 29 | 203 | 2.62e-41 | 139 |
| MS.gene053287.t1 | AT2G48140 | 50.331 | 151 | 63 | 6 | 1 | 140 | 15 | 164 | 5.44e-35 | 122 |
| MS.gene053287.t1 | AT2G48140 | 57.009 | 107 | 44 | 2 | 1 | 106 | 15 | 120 | 2.56e-34 | 119 |
Find 0 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTATTGAAATTGAAATT+TGG | - | 10823:9472-9491 | MS.gene053287:intergenic | 10.0% |
| !! | CATTATTAAGATTAATTCAA+AGG | - | 10823:9678-9697 | MS.gene053287:intergenic | 15.0% |
| !!! | GTTAGAAAATATTTTTGTTA+GGG | - | 10823:9530-9549 | MS.gene053287:intergenic | 15.0% |
| !!! | TGTTAGAAAATATTTTTGTT+AGG | - | 10823:9531-9550 | MS.gene053287:intergenic | 15.0% |
| ! | TCAAAGAAAAATGTAAGAGT+TGG | - | 10823:9888-9907 | MS.gene053287:intergenic | 25.0% |
| ! | TCAAAGGAAAAATAGTTTAG+AGG | - | 10823:9662-9681 | MS.gene053287:intergenic | 25.0% |
| !! | TTTTGTTAGGGATTATAGAA+GGG | - | 10823:9518-9537 | MS.gene053287:intergenic | 25.0% |
| !! | TTTTTGTTAGGGATTATAGA+AGG | - | 10823:9519-9538 | MS.gene053287:intergenic | 25.0% |
| !!! | CTATTGATTGGAATTTTGAA+GGG | - | 10823:9230-9249 | MS.gene053287:intergenic | 25.0% |
| !!! | TCTATTGATTGGAATTTTGA+AGG | - | 10823:9231-9250 | MS.gene053287:intergenic | 25.0% |
| !!! | TGATTTTGATGTATATTCAC+AGG | + | 10823:9696-9715 | MS.gene053287:intron | 25.0% |
| AATTGAAATTTGGCATTACC+AGG | - | 10823:9462-9481 | MS.gene053286:intergenic | 30.0% | |
| ATAGCTAAGGTTCTATTGAT+TGG | - | 10823:9242-9261 | MS.gene053287:intergenic | 30.0% | |
| CTATCATCTAGTAACATTGT+AGG | + | 10823:9629-9648 | MS.gene053287:intron | 30.0% | |
| TGAGACATTGTAAGATGTAA+TGG | - | 10823:10074-10093 | MS.gene053287:intergenic | 30.0% | |
| TTATTGTAGAAGGGTTACAT+GGG | - | 10823:9064-9083 | MS.gene053287:intergenic | 30.0% | |
| ! | TTTTTACCTTTGCATTGAAC+TGG | - | 10823:9299-9318 | MS.gene053287:intergenic | 30.0% |
| AGTGCAATCAATAGAACAGT+AGG | - | 10823:10097-10116 | MS.gene053287:intergenic | 35.0% | |
| CTTAAATCCCTAACAAGTAG+TGG | + | 10823:9167-9186 | MS.gene053287:CDS | 35.0% | |
| CTTATTGTAGAAGGGTTACA+TGG | - | 10823:9065-9084 | MS.gene053287:intergenic | 35.0% | |
| GTGTAATGCTAATAACCTTG+AGG | - | 10823:9789-9808 | MS.gene053287:intergenic | 35.0% | |
| TAAGATGTAATGGCAGATGA+TGG | - | 10823:10064-10083 | MS.gene053287:intergenic | 35.0% | |
| TATTGTAGAAGGGTTACATG+GGG | - | 10823:9063-9082 | MS.gene053287:intergenic | 35.0% | |
| ! | ACTGTTCTATTGATTGCACT+GGG | + | 10823:10097-10116 | MS.gene053287:CDS | 35.0% |
| ! | TACTGTTCTATTGATTGCAC+TGG | + | 10823:10096-10115 | MS.gene053287:CDS | 35.0% |
| !! | AAGAGAGTGCTTATTGTAGA+AGG | - | 10823:9074-9093 | MS.gene053287:intergenic | 35.0% |
| !! | AGAGAGTGCTTATTGTAGAA+GGG | - | 10823:9073-9092 | MS.gene053287:intergenic | 35.0% |
| AATCCATGCCACTACTTGTT+AGG | - | 10823:9178-9197 | MS.gene053286:intergenic | 40.0% | |
| ACTCTCTTCACACCTTGTAT+GGG | + | 10823:9086-9105 | MS.gene053287:CDS | 40.0% | |
| ATGTATATTCACAGGTCCTG+TGG | + | 10823:9704-9723 | MS.gene053287:intron | 40.0% | |
| ATTCCATCTACATCGACAAC+TGG | + | 10823:10016-10035 | MS.gene053287:CDS | 40.0% | |
| CCTGTAAGAAAACCCATACA+AGG | - | 10823:9101-9120 | MS.gene053287:intergenic | 40.0% | |
| CTAATAACCTTGAGGACTAG+GGG | - | 10823:9781-9800 | MS.gene053287:intergenic | 40.0% | |
| CTTACAGGTAGTAGTGCTAA+TGG | + | 10823:9113-9132 | MS.gene053287:CDS | 40.0% | |
| GCTAATAACCTTGAGGACTA+GGG | - | 10823:9782-9801 | MS.gene053287:intergenic | 40.0% | |
| TGCTAATAACCTTGAGGACT+AGG | - | 10823:9783-9802 | MS.gene053287:intergenic | 40.0% | |
| TGTCGATGTAGATGGAATAC+CGG | - | 10823:10014-10033 | MS.gene053287:intergenic | 40.0% | |
| ! | ATCCATGCCACTACTTGTTA+GGG | - | 10823:9177-9196 | MS.gene053287:intergenic | 40.0% |
| ! | CCTTGTATGGGTTTTCTTAC+AGG | + | 10823:9098-9117 | MS.gene053287:CDS | 40.0% |
| ! | GGAACATTTGTGTCTTGTTG+TGG | - | 10823:9959-9978 | MS.gene053287:intergenic | 40.0% |
| AATTTGGCATTACCAGGAGC+AGG | - | 10823:9456-9475 | MS.gene053286:intergenic | 45.0% | |
| AGGGAACACTAGCAGTTACA+AGG | - | 10823:9211-9230 | MS.gene053287:intergenic | 45.0% | |
| AGGGGTTGGAATAAATCCTG+AGG | - | 10823:9763-9782 | MS.gene053287:intergenic | 45.0% | |
| ATCCCTAACAAGTAGTGGCA+TGG | + | 10823:9172-9191 | MS.gene053287:CDS | 45.0% | |
| CACTCTCTTCACACCTTGTA+TGG | + | 10823:9085-9104 | MS.gene053287:CDS | 45.0% | |
| CTTCCAGTTGTCGATGTAGA+TGG | - | 10823:10022-10041 | MS.gene053287:intergenic | 45.0% | |
| GAAGATGCAGGTGAATGAGA+TGG | - | 10823:9738-9757 | MS.gene053287:intergenic | 45.0% | |
| GGGGTTGGAATAAATCCTGA+GGG | - | 10823:9762-9781 | MS.gene053287:intergenic | 45.0% | |
| GGTGTTCCAGTTCAATGCAA+AGG | + | 10823:9290-9309 | MS.gene053287:CDS | 45.0% | |
| TCACCAACCACTGAATGTTG+CGG | + | 10823:9140-9159 | MS.gene053287:CDS | 45.0% | |
| TCTACATCGACAACTGGAAG+CGG | + | 10823:10022-10041 | MS.gene053287:CDS | 45.0% | |
| TTCACCAACTTCATCTCCTC+TGG | + | 10823:9931-9950 | MS.gene053287:CDS | 45.0% | |
| TTGCATTGAACTGGAACACC+AGG | - | 10823:9290-9309 | MS.gene053287:intergenic | 45.0% | |
| !! | GAGATGAAGTTGGTGAAGCT+GGG | - | 10823:9928-9947 | MS.gene053287:intergenic | 45.0% |
| !! | TGGGGTGTTGATTTGTGCAT+TGG | - | 10823:9045-9064 | MS.gene053287:intergenic | 45.0% |
| AATCCTGAGGGAGAAGATGC+AGG | - | 10823:9750-9769 | MS.gene053286:intergenic | 50.0% | |
| ACGAGGGAGAGAGATAGCTA+AGG | - | 10823:9255-9274 | MS.gene053286:intergenic | 50.0% | |
| ATTCCAACCCCTAGTCCTCA+AGG | + | 10823:9771-9790 | MS.gene053287:CDS | 50.0% | |
| CAGGCATGTTACAAGCACGA+GGG | - | 10823:9271-9290 | MS.gene053287:intergenic | 50.0% | |
| GGAATACCGGAATTCACCGA+CGG | - | 10823:10001-10020 | MS.gene053287:intergenic | 50.0% | |
| TAACCTTGAGGACTAGGGGT+TGG | - | 10823:9777-9796 | MS.gene053287:intergenic | 50.0% | |
| ! | GCAACATTCAGTGGTTGGTG+AGG | - | 10823:9141-9160 | MS.gene053287:intergenic | 50.0% |
| !! | AAGAGCACCGCAACATTCAG+TGG | - | 10823:9150-9169 | MS.gene053287:intergenic | 50.0% |
| !! | GGAGATGAAGTTGGTGAAGC+TGG | - | 10823:9929-9948 | MS.gene053287:intergenic | 50.0% |
| AGCAGGAAGTGGTGAAGCTG+AGG | - | 10823:9439-9458 | MS.gene053287:intergenic | 55.0% | |
| CCAGGCATGTTACAAGCACG+AGG | - | 10823:9272-9291 | MS.gene053287:intergenic | 55.0% | |
| CCTCGTGCTTGTAACATGCC+TGG | + | 10823:9269-9288 | MS.gene053287:CDS | 55.0% | |
| GCACCGCAACATTCAGTGGT+TGG | - | 10823:9146-9165 | MS.gene053287:intergenic | 55.0% | |
| GCATTACCAGGAGCAGGAAG+TGG | - | 10823:9450-9469 | MS.gene053287:intergenic | 55.0% | |
| GGTGCCAGAGGAGATGAAGT+TGG | - | 10823:9938-9957 | MS.gene053287:intergenic | 55.0% | |
| TCACCTGCATCTTCTCCCTC+AGG | + | 10823:9744-9763 | MS.gene053287:CDS | 55.0% | |
| TGAGATGGTCCTAGAGCCAC+AGG | - | 10823:9723-9742 | MS.gene053287:intergenic | 55.0% | |
| TGGTGAAGCTGGGAGATCAG+AGG | - | 10823:9918-9937 | MS.gene053287:intergenic | 55.0% | |
| TTCACAGGTCCTGTGGCTCT+AGG | + | 10823:9711-9730 | MS.gene053287:intron | 55.0% | |
| ! | GTGTCTTGTTGTGGTGCCAG+AGG | - | 10823:9950-9969 | MS.gene053287:intergenic | 55.0% |
| !! | GGCGATGGCGGAGTCAAAAT+AGG | - | 10823:9980-9999 | MS.gene053287:intergenic | 55.0% |
| CCATCGCCGTCGGTGAATTC+CGG | + | 10823:9992-10011 | MS.gene053287:CDS | 60.0% | |
| GAATTCACCGACGGCGATGG+CGG | - | 10823:9992-10011 | MS.gene053287:intergenic | 60.0% | |
| GCTTCACCACTTCCTGCTCC+TGG | + | 10823:9441-9460 | MS.gene053287:CDS | 60.0% | |
| TTTGACTCCGCCATCGCCGT+CGG | + | 10823:9982-10001 | MS.gene053287:CDS | 60.0% | |
| CCGGAATTCACCGACGGCGA+TGG | - | 10823:9995-10014 | MS.gene053287:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10823 | gene | 9029 | 10144 | 9029 | ID=MS.gene053287 |
| 10823 | mRNA | 9029 | 10144 | 9029 | ID=MS.gene053287.t1;Parent=MS.gene053287 |
| 10823 | exon | 9029 | 9311 | 9029 | ID=MS.gene053287.t1.exon1;Parent=MS.gene053287.t1 |
| 10823 | CDS | 9029 | 9311 | 9029 | ID=cds.MS.gene053287.t1;Parent=MS.gene053287.t1 |
| 10823 | exon | 9436 | 9462 | 9436 | ID=MS.gene053287.t1.exon2;Parent=MS.gene053287.t1 |
| 10823 | CDS | 9436 | 9462 | 9436 | ID=cds.MS.gene053287.t1;Parent=MS.gene053287.t1 |
| 10823 | exon | 9718 | 9792 | 9718 | ID=MS.gene053287.t1.exon3;Parent=MS.gene053287.t1 |
| 10823 | CDS | 9718 | 9792 | 9718 | ID=cds.MS.gene053287.t1;Parent=MS.gene053287.t1 |
| 10823 | exon | 9912 | 10144 | 9912 | ID=MS.gene053287.t1.exon4;Parent=MS.gene053287.t1 |
| 10823 | CDS | 9912 | 10144 | 9912 | ID=cds.MS.gene053287.t1;Parent=MS.gene053287.t1 |
| Gene Sequence |
| Protein sequence |
