AlfalfaGEDB | MS.gene26946

M. sativa cultivar XinJiangDaYe / MS.gene26946

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26946.t1	XP_013466070.1	100	188	0	0	1	188	1	188	5.20E-103	383.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26946.t1	O49929	86.7	188	25	0	1	188	1	188	7.8e-92	337.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26946.t1	A0A072VDK2	100.0	188	0	0	1	188	1	188	3.7e-103	383.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054638	MS.gene26946	0.832205	1.08E-55	-1.69E-46
MS.gene057684	MS.gene26946	0.801026	1.09E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26946.t1	MTR_1g022050	100.000	188	0	0	1	188	1	188	1.08e-139	388

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26946.t1	AT1G45170	69.149	188	57	1	1	188	2	188	2.87e-96	278
MS.gene26946.t1	AT5G42960	67.021	188	60	2	1	188	3	188	1.17e-93	272
MS.gene26946.t1	AT1G45170	53.191	188	41	2	1	188	2	142	3.03e-62	191

Find 52 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCGAATTATGGTGTTGAATC+TGG	0.218241	1.3:+2996077	MS.gene26946:CDS
AGGGGATAACAGAACAATTT+TGG	0.234866	1.3:+2996016	MS.gene26946:CDS
AATAAGGTTTCAGCGAATTA+TGG	0.297969	1.3:+2996065	MS.gene26946:CDS
CAATTGTAGTCAAACCCTTA+TGG	0.311402	1.3:-2996127	None:intergenic
TTCAATTTATGAACACAGTT+AGG	0.317886	1.3:+2995952	MS.gene26946:CDS
GGCGACGGAGGCTGCGGCGG+CGG	0.338930	1.3:-2995699	None:intergenic
AACAATGAAAGCCGCTGTTA+AGG	0.348424	1.3:+2995652	None:intergenic
GCTGTTTCGAGGAAAGTTTA+TGG	0.364076	1.3:+2996188	MS.gene26946:CDS
ACAATGAAAGCCGCTGTTAA+GGG	0.378379	1.3:+2995653	None:intergenic
TGTGTGGGACCCATCGAATA+AGG	0.383360	1.3:+2996049	MS.gene26946:CDS
CGACTACAACGTCCCCAAAA+AGG	0.387317	1.3:+2995835	MS.gene26946:CDS
CAGACCACGAGTAAGAAGTT+AGG	0.393154	1.3:+2996236	MS.gene26946:CDS
ATTAACGGCCCAAGCTTAAC+CGG	0.393393	1.3:+2995773	MS.gene26946:CDS
ACAGACGCTACTTTCATTAA+CGG	0.400228	1.3:+2995758	MS.gene26946:CDS
GCCTCCGTCGCCTTCAACGC+CGG	0.402791	1.3:+2995710	MS.gene26946:CDS
TTTGACATCGCCGGCGTTGA+AGG	0.416806	1.3:-2995720	None:intergenic
TAACCTACATACACAGCAAA+GGG	0.443140	1.3:+2995997	MS.gene26946:CDS
GAAGTATAGCTATGTCCATA+AGG	0.446086	1.3:+2996112	MS.gene26946:CDS
TCAATTTATGAACACAGTTA+GGG	0.463428	1.3:+2995953	MS.gene26946:CDS
AGGACTAAGCCGGTTAAGCT+TGG	0.467012	1.3:-2995782	None:intergenic
GTAGTCGATACTGAAGGAAC+CGG	0.468560	1.3:-2995819	None:intergenic
AAGTATAGCTATGTCCATAA+GGG	0.470434	1.3:+2996113	MS.gene26946:CDS
GGACTAAGCCGGTTAAGCTT+GGG	0.473049	1.3:-2995781	None:intergenic
CGAATTATGGTGTTGAATCT+GGG	0.476174	1.3:+2996078	MS.gene26946:CDS
AGTTAGGGTTTGAGTGGTCG+AGG	0.482159	1.3:+2996252	MS.gene26946:CDS
ATCCCCTTTGCTGTGTATGT+AGG	0.496851	1.3:-2996000	None:intergenic
AGACCACGAGTAAGAAGTTA+GGG	0.497058	1.3:+2996237	MS.gene26946:CDS
TTCGCTGAAACCTTATTCGA+TGG	0.499631	1.3:-2996059	None:intergenic
GTTGAAGGCGACGGAGGCTG+CGG	0.500163	1.3:-2995705	None:intergenic
GTCGAGGAATTCGAAACAAA+GGG	0.505059	1.3:+2996268	MS.gene26946:CDS
TTCTACGGCGAGGACTAAGC+CGG	0.514419	1.3:-2995792	None:intergenic
TTATGGTGATGACTCATTGA+AGG	0.523909	1.3:+2996205	MS.gene26946:CDS
GAAGGCGACGGAGGCTGCGG+CGG	0.533980	1.3:-2995702	None:intergenic
TGCTCGGAGTTTGACATCGC+CGG	0.539580	1.3:-2995729	None:intergenic
TGTATGTAGGTTAAATTCAA+CGG	0.544412	1.3:-2995987	None:intergenic
TCGCTGAAACCTTATTCGAT+GGG	0.554144	1.3:-2996058	None:intergenic
TTCTTAGCAACATCATAACT+AGG	0.560239	1.3:-2996152	None:intergenic
TTTGGACGGAACATTTGTGT+GGG	0.592902	1.3:+2996034	MS.gene26946:CDS
ATCGCCGGCGTTGAAGGCGA+CGG	0.596432	1.3:-2995714	None:intergenic
GTCGTATTTGCCCTTAACAG+CGG	0.599655	1.3:-2995663	None:intergenic
TAGCGTCTGTTATCGATGCT+CGG	0.602349	1.3:-2995745	None:intergenic
GGTCGAGGAATTCGAAACAA+AGG	0.607936	1.3:+2996267	MS.gene26946:CDS
TAGTCGATACTGAAGGAACC+GGG	0.608857	1.3:-2995818	None:intergenic
TTAACCTACATACACAGCAA+AGG	0.618005	1.3:+2995996	MS.gene26946:CDS
GCCGGCGTTGAAGGCGACGG+AGG	0.618807	1.3:-2995711	None:intergenic
GTAAGAAGTTAGGGTTTGAG+TGG	0.628064	1.3:+2996246	MS.gene26946:CDS
TGATGTTGCTAAGAATGCGT+GGG	0.641670	1.3:+2996160	MS.gene26946:CDS
AAACCCTAACTTCTTACTCG+TGG	0.657874	1.3:-2996240	None:intergenic
ATGATGTTGCTAAGAATGCG+TGG	0.662892	1.3:+2996159	MS.gene26946:CDS
GACGTTGTAGTCGATACTGA+AGG	0.665017	1.3:-2995825	None:intergenic
AACCTACATACACAGCAAAG+GGG	0.729150	1.3:+2995998	MS.gene26946:CDS
TCGAGGAATTCGAAACAAAG+GGG	0.745078	1.3:+2996269	MS.gene26946:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAAATAAATAAATTGAAC+CGG	-	chr1.3:2995898-2995917	None:intergenic	10.0%
!!!	TTTTTTTTGTTTACCTTTTT+GGG	-	chr1.3:2995851-2995870	None:intergenic	15.0%
!!!	TTTTTTTTTGTTTACCTTTT+TGG	-	chr1.3:2995852-2995871	None:intergenic	15.0%
!!!	TTTTTTTGTTTACCTTTTTG+GGG	-	chr1.3:2995850-2995869	None:intergenic	20.0%
!	ATAAATAAATTGAACCGGTT+TGG	-	chr1.3:2995893-2995912	None:intergenic	25.0%
!	TCAATTTATGAACACAGTTA+GGG	+	chr1.3:2995953-2995972	MS.gene26946:CDS	25.0%
!	TGTATGTAGGTTAAATTCAA+CGG	-	chr1.3:2995990-2996009	None:intergenic	25.0%
!	TTCAATTTATGAACACAGTT+AGG	+	chr1.3:2995952-2995971	MS.gene26946:CDS	25.0%
!!!	TTTTTGTTTAATTTTGGTGC+AGG	+	chr1.3:2995920-2995939	MS.gene26946:intron	25.0%
	AAGTATAGCTATGTCCATAA+GGG	+	chr1.3:2996113-2996132	MS.gene26946:CDS	30.0%
	AATAAGGTTTCAGCGAATTA+TGG	+	chr1.3:2996065-2996084	MS.gene26946:CDS	30.0%
	TTCTTAGCAACATCATAACT+AGG	-	chr1.3:2996155-2996174	None:intergenic	30.0%
!	ATCCATTTTCTAAACCAAAC+CGG	+	chr1.3:2995876-2995895	MS.gene26946:intron	30.0%
!	GATAACAGAACAATTTTGGA+CGG	+	chr1.3:2996020-2996039	MS.gene26946:CDS	30.0%
!!	TAATTTTGGTGCAGGATTTT+AGG	+	chr1.3:2995928-2995947	MS.gene26946:intron	30.0%
	CAATTGTAGTCAAACCCTTA+TGG	-	chr1.3:2996130-2996149	None:intergenic	35.0%
	GAAGTATAGCTATGTCCATA+AGG	+	chr1.3:2996112-2996131	MS.gene26946:CDS	35.0%
	TAACCTACATACACAGCAAA+GGG	+	chr1.3:2995997-2996016	MS.gene26946:CDS	35.0%
	TTAACCTACATACACAGCAA+AGG	+	chr1.3:2995996-2996015	MS.gene26946:CDS	35.0%
!	ACAGACGCTACTTTCATTAA+CGG	+	chr1.3:2995758-2995777	MS.gene26946:CDS	35.0%
!	AGGGGATAACAGAACAATTT+TGG	+	chr1.3:2996016-2996035	MS.gene26946:CDS	35.0%
!	CGAATTATGGTGTTGAATCT+GGG	+	chr1.3:2996078-2996097	MS.gene26946:CDS	35.0%
!	TTATGGTGATGACTCATTGA+AGG	+	chr1.3:2996205-2996224	MS.gene26946:CDS	35.0%
!!	AACCGGTTTGGTTTAGAAAA+TGG	-	chr1.3:2995881-2995900	None:intergenic	35.0%
!!	GAAACAAAGGGGTTGTTTTA+AGG	+	chr1.3:2996280-2996299	MS.gene26946:CDS	35.0%
	AAACCCTAACTTCTTACTCG+TGG	-	chr1.3:2996243-2996262	None:intergenic	40.0%
	AACCTACATACACAGCAAAG+GGG	+	chr1.3:2995998-2996017	MS.gene26946:CDS	40.0%
	AGACCACGAGTAAGAAGTTA+GGG	+	chr1.3:2996237-2996256	MS.gene26946:CDS	40.0%
	ATGATGTTGCTAAGAATGCG+TGG	+	chr1.3:2996159-2996178	MS.gene26946:CDS	40.0%
	GTAAGAAGTTAGGGTTTGAG+TGG	+	chr1.3:2996246-2996265	MS.gene26946:CDS	40.0%
	GTCGAGGAATTCGAAACAAA+GGG	+	chr1.3:2996268-2996287	MS.gene26946:CDS	40.0%
	TCGAGGAATTCGAAACAAAG+GGG	+	chr1.3:2996269-2996288	MS.gene26946:CDS	40.0%
	TCGCTGAAACCTTATTCGAT+GGG	-	chr1.3:2996061-2996080	None:intergenic	40.0%
	TGATGTTGCTAAGAATGCGT+GGG	+	chr1.3:2996160-2996179	MS.gene26946:CDS	40.0%
	TTCGCTGAAACCTTATTCGA+TGG	-	chr1.3:2996062-2996081	None:intergenic	40.0%
	TTTGGACGGAACATTTGTGT+GGG	+	chr1.3:2996034-2996053	MS.gene26946:CDS	40.0%
!	GCGAATTATGGTGTTGAATC+TGG	+	chr1.3:2996077-2996096	MS.gene26946:CDS	40.0%
!	GCTGTTTCGAGGAAAGTTTA+TGG	+	chr1.3:2996188-2996207	MS.gene26946:CDS	40.0%
!	TTTTGGACGGAACATTTGTG+TGG	+	chr1.3:2996033-2996052	MS.gene26946:CDS	40.0%
	ATTAACGGCCCAAGCTTAAC+CGG	+	chr1.3:2995773-2995792	MS.gene26946:CDS	45.0%
	CAGACCACGAGTAAGAAGTT+AGG	+	chr1.3:2996236-2996255	MS.gene26946:CDS	45.0%
	GACGTTGTAGTCGATACTGA+AGG	-	chr1.3:2995828-2995847	None:intergenic	45.0%
	GGTCGAGGAATTCGAAACAA+AGG	+	chr1.3:2996267-2996286	MS.gene26946:CDS	45.0%
	GTAGTCGATACTGAAGGAAC+CGG	-	chr1.3:2995822-2995841	None:intergenic	45.0%
	GTCGTATTTGCCCTTAACAG+CGG	-	chr1.3:2995666-2995685	None:intergenic	45.0%
	TAGCGTCTGTTATCGATGCT+CGG	-	chr1.3:2995748-2995767	None:intergenic	45.0%
	TAGTCGATACTGAAGGAACC+GGG	-	chr1.3:2995821-2995840	None:intergenic	45.0%
!	ATCCCCTTTGCTGTGTATGT+AGG	-	chr1.3:2996003-2996022	None:intergenic	45.0%
!	GAAGGAACCGGGTTTTTCTA+CGG	-	chr1.3:2995810-2995829	None:intergenic	45.0%
!!!	TATTTATTTTTGTTTAATTT+TGG	+	chr1.3:2995914-2995933	MS.gene26946:intron	5.0%
	AGGACTAAGCCGGTTAAGCT+TGG	-	chr1.3:2995785-2995804	None:intergenic	50.0%
	AGTTAGGGTTTGAGTGGTCG+AGG	+	chr1.3:2996252-2996271	MS.gene26946:CDS	50.0%
	CGACTACAACGTCCCCAAAA+AGG	+	chr1.3:2995835-2995854	MS.gene26946:CDS	50.0%
	GGACTAAGCCGGTTAAGCTT+GGG	-	chr1.3:2995784-2995803	None:intergenic	50.0%
	TGTGTGGGACCCATCGAATA+AGG	+	chr1.3:2996049-2996068	MS.gene26946:CDS	50.0%
!!!	CGTGGGATTTTGCTGTTTCG+AGG	+	chr1.3:2996177-2996196	MS.gene26946:CDS	50.0%
	GTCCTCGCCGTAGAAAAACC+CGG	+	chr1.3:2995800-2995819	MS.gene26946:CDS	55.0%
	TGCTCGGAGTTTGACATCGC+CGG	-	chr1.3:2995732-2995751	None:intergenic	55.0%
	TTCTACGGCGAGGACTAAGC+CGG	-	chr1.3:2995795-2995814	None:intergenic	55.0%
!	AACCGGGTTTTTCTACGGCG+AGG	-	chr1.3:2995805-2995824	None:intergenic	55.0%
!	TTTGACATCGCCGGCGTTGA+AGG	-	chr1.3:2995723-2995742	None:intergenic	55.0%
	GTTGAAGGCGACGGAGGCTG+CGG	-	chr1.3:2995708-2995727	None:intergenic	65.0%
!	ATCGCCGGCGTTGAAGGCGA+CGG	-	chr1.3:2995717-2995736	None:intergenic	65.0%
	GCCTCCGTCGCCTTCAACGC+CGG	+	chr1.3:2995710-2995729	MS.gene26946:CDS	70.0%
	GAAGGCGACGGAGGCTGCGG+CGG	-	chr1.3:2995705-2995724	None:intergenic	75.0%
!	GCCGGCGTTGAAGGCGACGG+AGG	-	chr1.3:2995714-2995733	None:intergenic	75.0%
!	GGCGACGGAGGCTGCGGCGG+CGG	-	chr1.3:2995702-2995721	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	2995656	2996313	2995656	ID=MS.gene26946
chr1.3	mRNA	2995656	2996313	2995656	ID=MS.gene26946.t1;Parent=MS.gene26946
chr1.3	exon	2995656	2995856	2995656	ID=MS.gene26946.t1.exon1;Parent=MS.gene26946.t1
chr1.3	CDS	2995656	2995856	2995656	ID=cds.MS.gene26946.t1;Parent=MS.gene26946.t1
chr1.3	exon	2995942	2996313	2995942	ID=MS.gene26946.t1.exon2;Parent=MS.gene26946.t1
chr1.3	CDS	2995942	2996313	2995942	ID=cds.MS.gene26946.t1;Parent=MS.gene26946.t1

Gene Sequence

>MS.gene26946

ATGAAAGCCGCTGTTAAGGGCAAATACGACAATGACAAAAACGCCGCCGCCGCAGCCTCCGTCGCCTTCAACGCCGGCGATGTCAAACTCCGAGCATCGATAACAGACGCTACTTTCATTAACGGCCCAAGCTTAACCGGCTTAGTCCTCGCCGTAGAAAAACCCGGTTCCTTCAGTATCGACTACAACGTCCCCAAAAAGGTAAACAAAAAAAAATTCAATCCATTTTCTAAACCAAACCGGTTCAATTTATTTATTTATTTATTTTTGTTTAATTTTGGTGCAGGATTTTAGGTTTCAATTTATGAACACAGTTAGGGTTGCTGATAAACCGTTGAATTTAACCTACATACACAGCAAAGGGGATAACAGAACAATTTTGGACGGAACATTTGTGTGGGACCCATCGAATAAGGTTTCAGCGAATTATGGTGTTGAATCTGGGAATTGTAGATTGAAGTATAGCTATGTCCATAAGGGTTTGACTACAATTGAACCTAGTTATGATGTTGCTAAGAATGCGTGGGATTTTGCTGTTTCGAGGAAAGTTTATGGTGATGACTCATTGAAGGCGACGTATCAGACCACGAGTAAGAAGTTAGGGTTTGAGTGGTCGAGGAATTCGAAACAAAGGGGTTGTTTTAAGGTTAGTTGTTGA

Protein sequence

>MS.gene26946.t1

MKAAVKGKYDNDKNAAAAASVAFNAGDVKLRASITDATFINGPSLTGLVLAVEKPGSFSIDYNVPKKDFRFQFMNTVRVADKPLNLTYIHSKGDNRTILDGTFVWDPSNKVSANYGVESGNCRLKYSYVHKGLTTIEPSYDVAKNAWDFAVSRKVYGDDSLKATYQTTSKKLGFEWSRNSKQRGCFKVSC