AlfalfaGEDB | MS.gene27679

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27679.t1	XP_003623880.1	100	62	0	0	1	62	1	62	3.60E-28	133.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27679.t1	G7L2K4	100.0	62	0	0	1	62	1	62	2.6e-28	133.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049341	MS.gene27679	0.80162	8.26E-49	-1.69E-46
MS.gene051328	MS.gene27679	0.803088	4.12E-49	-1.69E-46
MS.gene058906	MS.gene27679	0.802391	5.74E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27679.t1	MTR_7g076630	100.000	62	0	0	1	62	1	62	2.30e-41	130

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27679.t1	AT4G00585	83.051	59	9	1	4	62	2	59	5.62e-30	101

Find 22 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGTTGCGTTTCCAGTGTTT+AGG	0.292140	7.2:-29080891	None:intergenic
GAGCCAATGGAGGAGATTTC+AGG	0.296342	7.2:+29080829	MS.gene27679:CDS
TGTTGCGTTTCCAGTGTTTA+GGG	0.348623	7.2:-29080890	None:intergenic
GAGATTTCAGGTACAAGGTT+TGG	0.375661	7.2:+29080841	MS.gene27679:CDS
TTTAGCTGAGAGTTTGAAGA+TGG	0.461581	7.2:-29080952	None:intergenic
CACGGTAATGGCGGAGCCAA+TGG	0.484941	7.2:+29080816	MS.gene27679:CDS
TAGCTGAGAGTTTGAAGATG+GGG	0.490985	7.2:-29080950	None:intergenic
GTACCTGAAATCTCCTCCAT+TGG	0.492681	7.2:-29080832	None:intergenic
AACACTGCCATCGCTATGTT+CGG	0.493133	7.2:+29080909	MS.gene27679:CDS
TGGAGGAGATTTCAGGTACA+AGG	0.512900	7.2:+29080836	MS.gene27679:CDS
TTAGCTGAGAGTTTGAAGAT+GGG	0.520876	7.2:-29080951	None:intergenic
GGTGGAGCAGATCACGGTAA+TGG	0.524664	7.2:+29080804	MS.gene27679:CDS
TACAAGGTTTGGAGTATGAC+TGG	0.533279	7.2:+29080852	MS.gene27679:CDS
CTCACTGTCGCCCTAAACAC+TGG	0.552111	7.2:+29080880	MS.gene27679:CDS
CAAACTCTCAGCTAAACTTG+AGG	0.608144	7.2:+29080959	MS.gene27679:CDS
GGAGGTGGTGGAGCAGATCA+CGG	0.622572	7.2:+29080798	MS.gene27679:CDS
GGTAATGGCGGAGCCAATGG+AGG	0.641548	7.2:+29080819	MS.gene27679:CDS
GACGATACCGAACATAGCGA+TGG	0.647243	7.2:-29080916	None:intergenic
GGAGCAGATCACGGTAATGG+CGG	0.651187	7.2:+29080807	MS.gene27679:CDS
CAGTGTTTAGGGCGACAGTG+AGG	0.708119	7.2:-29080879	None:intergenic
AAGGTTTGGAGTATGACTGG+TGG	0.712549	7.2:+29080855	MS.gene27679:CDS
TGAAGATGGGGATACAAACG+AGG	0.746465	7.2:-29080938	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAATCAAAACATGGAAATC+TGG	-	chr7.2:29081027-29081046	None:intergenic	25.0%
!	GGAGATATAAAATCAAAACA+TGG	-	chr7.2:29081035-29081054	None:intergenic	25.0%
	GGAAATCTGGTAAAGAAAAC+AGG	-	chr7.2:29081014-29081033	None:intergenic	35.0%
	TTAGCTGAGAGTTTGAAGAT+GGG	-	chr7.2:29080954-29080973	None:intergenic	35.0%
	TTTAGCTGAGAGTTTGAAGA+TGG	-	chr7.2:29080955-29080974	None:intergenic	35.0%
	CAAACTCTCAGCTAAACTTG+AGG	+	chr7.2:29080959-29080978	MS.gene27679:CDS	40.0%
	TAGCTGAGAGTTTGAAGATG+GGG	-	chr7.2:29080953-29080972	None:intergenic	40.0%
	TGTTGCGTTTCCAGTGTTTA+GGG	-	chr7.2:29080893-29080912	None:intergenic	40.0%
!	GAGATTTCAGGTACAAGGTT+TGG	+	chr7.2:29080841-29080860	MS.gene27679:CDS	40.0%
!	TACAAGGTTTGGAGTATGAC+TGG	+	chr7.2:29080852-29080871	MS.gene27679:CDS	40.0%
	AACACTGCCATCGCTATGTT+CGG	+	chr7.2:29080909-29080928	MS.gene27679:CDS	45.0%
	GTACCTGAAATCTCCTCCAT+TGG	-	chr7.2:29080835-29080854	None:intergenic	45.0%
	GTGTTGCGTTTCCAGTGTTT+AGG	-	chr7.2:29080894-29080913	None:intergenic	45.0%
	TGAAGATGGGGATACAAACG+AGG	-	chr7.2:29080941-29080960	None:intergenic	45.0%
!	AAGGTTTGGAGTATGACTGG+TGG	+	chr7.2:29080855-29080874	MS.gene27679:CDS	45.0%
!	TGGAGGAGATTTCAGGTACA+AGG	+	chr7.2:29080836-29080855	MS.gene27679:CDS	45.0%
!!!	AAAAAAAATTGATAATAATT+TGG	-	chr7.2:29081056-29081075	None:intergenic	5.0%
!!!	AATTATTATCAATTTTTTTT+AGG	+	chr7.2:29081056-29081075	MS.gene27679:intron	5.0%
!!!	ATTATTATCAATTTTTTTTA+GGG	+	chr7.2:29081057-29081076	MS.gene27679:intron	5.0%
	GACGATACCGAACATAGCGA+TGG	-	chr7.2:29080919-29080938	None:intergenic	50.0%
	GAGCCAATGGAGGAGATTTC+AGG	+	chr7.2:29080829-29080848	MS.gene27679:CDS	50.0%
	CTCACTGTCGCCCTAAACAC+TGG	+	chr7.2:29080880-29080899	MS.gene27679:CDS	55.0%
	GGTGGAGCAGATCACGGTAA+TGG	+	chr7.2:29080804-29080823	MS.gene27679:CDS	55.0%
!	CAGTGTTTAGGGCGACAGTG+AGG	-	chr7.2:29080882-29080901	None:intergenic	55.0%
!	GGAGCAGATCACGGTAATGG+CGG	+	chr7.2:29080807-29080826	MS.gene27679:CDS	55.0%
!	CACGGTAATGGCGGAGCCAA+TGG	+	chr7.2:29080816-29080835	MS.gene27679:CDS	60.0%
!	GGTAATGGCGGAGCCAATGG+AGG	+	chr7.2:29080819-29080838	MS.gene27679:CDS	60.0%
!!	GGAGGTGGTGGAGCAGATCA+CGG	+	chr7.2:29080798-29080817	MS.gene27679:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	29080795	29081113	29080795	ID=MS.gene27679
chr7.2	mRNA	29080795	29081113	29080795	ID=MS.gene27679.t1;Parent=MS.gene27679
chr7.2	exon	29080795	29080980	29080795	ID=MS.gene27679.t1.exon1;Parent=MS.gene27679.t1
chr7.2	CDS	29080795	29080980	29080795	ID=cds.MS.gene27679.t1;Parent=MS.gene27679.t1
chr7.2	exon	29081078	29081113	29081078	ID=MS.gene27679.t1.exon2;Parent=MS.gene27679.t1
chr7.2	CDS	29081078	29081113	29081078	ID=cds.MS.gene27679.t1;Parent=MS.gene27679.t1

Gene Sequence	>MS.gene27679 ATGGGAGGTGGTGGAGCAGATCACGGTAATGGCGGAGCCAATGGAGGAGATTTCAGGTACAAGGTTTGGAGTATGACTGGTGGTCCTCACTGTCGCCCTAAACACTGGAAACGCAACACTGCCATCGCTATGTTCGGTATCGTCCTCGTTTGTATCCCCATCTTCAAACTCTCAGCTAAACTTGAGGTTCTTCATTCTCTCTTTCTCAATCTAATTCCTGTTTTCTTTACCAGATTTCCATGTTTTGATTTTATATCTCCAAATTATTATCAATTTTTTTTAGGGAAATTTTGTGTTTTTGTATTATGTTTTTGATTAA
Protein sequence	>MS.gene27679.t1 MGGGGADHGNGGANGGDFRYKVWSMTGGPHCRPKHWKRNTAIAMFGIVLVCIPIFKLSAKLEGNFVFLYYVFD

M. sativa cultivar XinJiangDaYe / MS.gene27679