AlfalfaGEDB | MS.gene27993

M. sativa cultivar XinJiangDaYe / MS.gene27993

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27993.t1	XP_013446712.1	97.9	143	3	0	1	143	1	143	2.60E-67	264.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27993.t1	A0A072TT93	97.9	143	3	0	1	143	1	143	1.9e-67	264.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055603	MS.gene27993	0.822913	1.84E-53	-1.69E-46
MS.gene056403	MS.gene27993	0.832846	7.49E-56	-1.69E-46
MS.gene056605	MS.gene27993	0.839378	1.64E-57	-1.69E-46
MS.gene058911	MS.gene27993	0.802853	4.61E-49	-1.69E-46
MS.gene060830	MS.gene27993	0.815347	9.68E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27993.t1	MTR_8g089655	97.902	143	3	0	1	143	4	146	4.61e-99	281
MS.gene27993.t1	MTR_8g089655	97.902	143	3	0	1	143	1	143	5.79e-99	280

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27993.t1	AT1G16790	54.464	112	48	2	25	134	24	134	6.18e-41	134

Find 34 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGCAATTGGGGTTGTTTC+TGG	0.158557	4.4:-11666757	MS.gene27993:CDS
GTTTCTGGTGCTGCTCTTAA+TGG	0.209111	4.4:-11666742	MS.gene27993:CDS
CATTCCTTGTGATTCATAAT+AGG	0.265305	4.4:+11666966	None:intergenic
TTCATCTGGTAAAGGTAAAA+AGG	0.279247	4.4:-11666881	MS.gene27993:CDS
TTTCACCTATTGCTCAGATT+TGG	0.309135	4.4:-11666693	MS.gene27993:CDS
GATTTACAGAAGGCGTTGTT+TGG	0.358146	4.4:-11666910	MS.gene27993:CDS
GGTGCTGCTCTTAATGGAAT+TGG	0.362930	4.4:-11666736	MS.gene27993:CDS
TTGTTGAGACGTTTGCAATT+GGG	0.377010	4.4:-11666768	MS.gene27993:CDS
TAATAGGATATCAATTCCTT+TGG	0.387539	4.4:+11666982	None:intergenic
TCAGGTATAGAAGGTACATT+TGG	0.388079	4.4:+11667045	None:intergenic
TTTGTTGAGACGTTTGCAAT+TGG	0.400517	4.4:-11666769	MS.gene27993:CDS
GAATTCCAAATCTGAGCAAT+AGG	0.408372	4.4:+11666688	None:intergenic
TTGTTTGGAGTAATTTCATC+TGG	0.412151	4.4:-11666895	MS.gene27993:CDS
AAATGAATCTTCTAGAAAGA+TGG	0.419369	4.4:-11666848	MS.gene27993:CDS
TCATTGAGGGGTAGATTTAG+TGG	0.436309	4.4:+11666654	None:intergenic
ATGAAGCTTGATACTAAGCC+TGG	0.457946	4.4:-11666793	MS.gene27993:CDS
GGAGTAATTTCATCTGGTAA+AGG	0.473380	4.4:-11666889	MS.gene27993:CDS
TATAGAAGGTACATTTGGCT+TGG	0.481909	4.4:+11667050	None:intergenic
AGGGTACAAAATGTCATTGA+GGG	0.507238	4.4:+11666641	None:intergenic
TGTTGAGACGTTTGCAATTG+GGG	0.507433	4.4:-11666767	MS.gene27993:CDS
ATCTTCTAGAAAGATGGATT+CGG	0.522036	4.4:-11666842	MS.gene27993:CDS
CTATTATGAATCACAAGGAA+TGG	0.524935	4.4:-11666965	MS.gene27993:CDS
AGGGGTAGATTTAGTGGCTG+AGG	0.534389	4.4:+11666660	None:intergenic
TTCACAAGAAGCCTCATTGA+AGG	0.549868	4.4:-11666941	MS.gene27993:CDS
AAGGGTACAAAATGTCATTG+AGG	0.555917	4.4:+11666640	None:intergenic
GCAAACGTCTCAACAAATCC+AGG	0.561055	4.4:+11666775	None:intergenic
GATATCAATTCCTTTGGCGA+AGG	0.568099	4.4:+11666988	None:intergenic
AAAGAAGATTCCTTCGCCAA+AGG	0.596578	4.4:-11666998	MS.gene27993:CDS
AGCCTCATTGAAGGTCATAG+AGG	0.621483	4.4:-11666932	MS.gene27993:CDS
GGTCATAGAGGATTTACAGA+AGG	0.636264	4.4:-11666920	MS.gene27993:CDS
GGGTACAAAATGTCATTGAG+GGG	0.657891	4.4:+11666642	None:intergenic
ATATCCTATTATGAATCACA+AGG	0.666482	4.4:-11666970	MS.gene27993:CDS
ATCCTCTATGACCTTCAATG+AGG	0.666837	4.4:+11666930	None:intergenic
GCAATAGGTGAAAGAATGTG+AGG	0.689368	4.4:+11666703	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTGTTTCATTTTGATTTC+AGG	+	chr4.4:11666693-11666712	None:intergenic	20.0%
!	ATATCCTATTATGAATCACA+AGG	-	chr4.4:11666747-11666766	MS.gene27993:CDS	25.0%
!	TAATAGGATATCAATTCCTT+TGG	+	chr4.4:11666738-11666757	None:intergenic	25.0%
!!	AAATGAATCTTCTAGAAAGA+TGG	-	chr4.4:11666869-11666888	MS.gene27993:CDS	25.0%
!!!	ATTTTGATTTCAGGTATAGA+AGG	+	chr4.4:11666684-11666703	None:intergenic	25.0%
	CATTCCTTGTGATTCATAAT+AGG	+	chr4.4:11666754-11666773	None:intergenic	30.0%
	CTATTATGAATCACAAGGAA+TGG	-	chr4.4:11666752-11666771	MS.gene27993:CDS	30.0%
	TTCATCTGGTAAAGGTAAAA+AGG	-	chr4.4:11666836-11666855	MS.gene27993:CDS	30.0%
!	ATCTTCTAGAAAGATGGATT+CGG	-	chr4.4:11666875-11666894	MS.gene27993:CDS	30.0%
!	TTGTTTGGAGTAATTTCATC+TGG	-	chr4.4:11666822-11666841	MS.gene27993:CDS	30.0%
	CTGGTAAAGGTAAAAAGGAT+AGG	-	chr4.4:11666841-11666860	MS.gene27993:CDS	35.0%
	GAATTCCAAATCTGAGCAAT+AGG	+	chr4.4:11667032-11667051	None:intergenic	35.0%
	GGAGTAATTTCATCTGGTAA+AGG	-	chr4.4:11666828-11666847	MS.gene27993:CDS	35.0%
	TTGTTGAGACGTTTGCAATT+GGG	-	chr4.4:11666949-11666968	MS.gene27993:CDS	35.0%
	TTTCACCTATTGCTCAGATT+TGG	-	chr4.4:11667024-11667043	MS.gene27993:CDS	35.0%
	TTTGTTGAGACGTTTGCAAT+TGG	-	chr4.4:11666948-11666967	MS.gene27993:CDS	35.0%
!	TATAGAAGGTACATTTGGCT+TGG	+	chr4.4:11666670-11666689	None:intergenic	35.0%
!	TCAGGTATAGAAGGTACATT+TGG	+	chr4.4:11666675-11666694	None:intergenic	35.0%
	AAAGAAGATTCCTTCGCCAA+AGG	-	chr4.4:11666719-11666738	MS.gene27993:CDS	40.0%
	ATCCTCTATGACCTTCAATG+AGG	+	chr4.4:11666790-11666809	None:intergenic	40.0%
	ATGAAGCTTGATACTAAGCC+TGG	-	chr4.4:11666924-11666943	MS.gene27993:CDS	40.0%
	GATATCAATTCCTTTGGCGA+AGG	+	chr4.4:11666732-11666751	None:intergenic	40.0%
	GCAATAGGTGAAAGAATGTG+AGG	+	chr4.4:11667017-11667036	None:intergenic	40.0%
	GGTCATAGAGGATTTACAGA+AGG	-	chr4.4:11666797-11666816	MS.gene27993:CDS	40.0%
	TCATTGAGGGGTAGATTTAG+TGG	+	chr4.4:11667066-11667085	None:intergenic	40.0%
	TGTTGAGACGTTTGCAATTG+GGG	-	chr4.4:11666950-11666969	MS.gene27993:CDS	40.0%
	TTCACAAGAAGCCTCATTGA+AGG	-	chr4.4:11666776-11666795	MS.gene27993:CDS	40.0%
!	TTTGCAATTGGGGTTGTTTC+TGG	-	chr4.4:11666960-11666979	MS.gene27993:CDS	40.0%
!!	GATTTACAGAAGGCGTTGTT+TGG	-	chr4.4:11666807-11666826	MS.gene27993:CDS	40.0%
	AGCCTCATTGAAGGTCATAG+AGG	-	chr4.4:11666785-11666804	MS.gene27993:CDS	45.0%
	GCAAACGTCTCAACAAATCC+AGG	+	chr4.4:11666945-11666964	None:intergenic	45.0%
!!	GGTGCTGCTCTTAATGGAAT+TGG	-	chr4.4:11666981-11667000	MS.gene27993:CDS	45.0%
!!	GTTTCTGGTGCTGCTCTTAA+TGG	-	chr4.4:11666975-11666994	MS.gene27993:CDS	45.0%
	AGGGGTAGATTTAGTGGCTG+AGG	+	chr4.4:11667060-11667079	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	11666654	11667085	11666654	ID=MS.gene27993
chr4.4	mRNA	11666654	11667085	11666654	ID=MS.gene27993.t1;Parent=MS.gene27993
chr4.4	exon	11666654	11667085	11666654	ID=MS.gene27993.t1.exon1;Parent=MS.gene27993.t1
chr4.4	CDS	11666654	11667085	11666654	ID=cds.MS.gene27993.t1;Parent=MS.gene27993.t1

Gene Sequence

>MS.gene27993

ATGGAACAATCTTCCAAGCCAAATGTACCTTCTATACCTGAAATCAAAATGAAACAAAATCAGAAAAAGAAGATTCCTTCGCCAAAGGAATTGATATCCTATTATGAATCACAAGGAATGGATTCACAAGAAGCCTCATTGAAGGTCATAGAGGATTTACAGAAGGCGTTGTTTGGAGTAATTTCATCTGGTAAAGGTAAAAAGGATAGGCTTATAAATGAATCTTCTAGAAAGATGGATTCGGTTAATCAAAGACTTGCTATACTTGATATGAAGCTTGATACTAAGCCTGGATTTGTTGAGACGTTTGCAATTGGGGTTGTTTCTGGTGCTGCTCTTAATGGAATTGGTGCTATAATGCCTCACATTCTTTCACCTATTGCTCAGATTTGGAATTCTGTTACCTCAGCCACTAAATCTACCCCTCAATGA

Protein sequence

>MS.gene27993.t1

MEQSSKPNVPSIPEIKMKQNQKKKIPSPKELISYYESQGMDSQEASLKVIEDLQKALFGVISSGKGKKDRLINESSRKMDSVNQRLAILDMKLDTKPGFVETFAIGVVSGAALNGIGAIMPHILSPIAQIWNSVTSATKSTPQ